% Generated by roxygen2: do not edit by hand
% Please edit documentation in R/plotFeatureClasses.R
\name{plotFeatureClasses}
\alias{plotFeatureClasses}
\alias{plotFeatureClasses,matrix-method}
\alias{plotFeatureClasses,DataFrame-method}
\alias{plotFeatureClasses,MultiAssayExperiment-method}
\title{Plot Density, Scatterplot, Parallel Plot or Bar Chart for Features By Class}
\usage{
\S4method{plotFeatureClasses}{matrix}(measurements, ...)
\S4method{plotFeatureClasses}{DataFrame}(
measurements,
classes,
useFeatures,
groupBy = NULL,
groupingName = NULL,
whichNumericFeaturePlots = c("both", "density", "stripchart"),
measurementLimits = NULL,
lineWidth = 1,
dotBinWidth = 1,
xAxisLabel = NULL,
yAxisLabels = c("Density", "Classes"),
showXtickLabels = TRUE,
showYtickLabels = TRUE,
xLabelPositions = "auto",
yLabelPositions = "auto",
fontSizes = c(24, 16, 12, 12, 12),
colours = c("#3F48CC", "#880015"),
showAssayName = TRUE
)
\S4method{plotFeatureClasses}{MultiAssayExperiment}(
measurements,
useFeatures,
classesColumn,
groupBy = NULL,
groupingName = NULL,
showAssayName = TRUE,
...
)
}
\arguments{
\item{measurements}{A \code{\link{matrix}}, \code{\link{DataFrame}} or a
\code{\link{MultiAssayExperiment}} object containing the data. For a
matrix, the rows are for features and the columns are for samples. A column
with name \code{"class"} must be present in the \code{DataFrame} stored in
the \code{colData} slot.}
\item{...}{Unused variables by the three top-level methods passed to the
internal method which generates the plot(s).}
\item{classes}{Either a vector of class labels of class \code{\link{factor}}
or if the measurements are of class \code{DataFrame} a character vector of
length 1 containing the column name in \code{measurement} is also permitted.
Not used if \code{measurements} is a \code{MultiAssayExperiment} object.}
\item{useFeatures}{If \code{measurements} is a \code{matrix} or
\code{DataFrame}, then a vector of numeric or character indices or the
feature identifiers corresponding to the feature(s) to be plotted. If
\code{measurements} is a \code{MultiAssayExperiment}, then a
\code{DataFrame} of 2 columns must be specified. The first column contains
the names of the assays and the second contains the names of the variables,
thus each row unambiguously specifies a variable to be plotted.}
\item{groupBy}{If \code{measurements} is a \code{DataFrame}, then a
character vector of length 1, which contains the name of a categorical
feature, may be specified. If \code{measurements} is a
\code{MultiAssayExperiment}, then a character vector of length 2, which
contains the name of a data table as the first element and the name of a
categorical feature as the second element, may be specified. Additionally,
the value \code{"clinical"} may be used to refer to the column annotation
stored in the \code{colData} slot of the of the \code{MultiAssayExperiment}
object. A density plot will have additional lines of different line types
for each category. A strip chart plot will have a separate strip chart
created for each category and the charts will be drawn in a single column on
the graphics device. A parallel plot and bar chart plot will similarly be
laid out.}
\item{groupingName}{A label for the grouping variable to be used in plots.}
\item{whichNumericFeaturePlots}{If the feature is a single feature and has
numeric measurements, this option specifies which types of plot(s) to draw.
The default value is \code{"both"}, which draws a density plot and also a
stip chart below the density plot. Other options are \code{"density"} for
drawing only a density plot and \code{"stripchart"} for drawing only a strip
chart.}
\item{measurementLimits}{The minimum and maximum expression values to plot.
Default: \code{NULL}. By default, the limits are automatically computed
from the data values.}
\item{lineWidth}{Numeric value that alters the line thickness for density
plots. Default: 1.}
\item{dotBinWidth}{Numeric value that alters the diameter of dots in the
strip chart. Default: 1.}
\item{xAxisLabel}{The axis label for the plot's horizontal axis. Default:
\code{NULL}.}
\item{yAxisLabels}{A character vector of length 1 or 2. If the feature's
measurements are numeric an \code{whichNumericFeaturePlots} has the value
\code{"both"}, the first value is the y-axis label for the density plot and
the second value is the y-axis label for the strip chart. Otherwise, if the
feature's measurements are numeric and only one plot is drawn, then a
character vector of length 1 specifies the y-axis label for that particular
plot. Ignored if the feature's measurements are categorical.}
\item{showXtickLabels}{Logical. Default: \code{TRUE}. If set to
\code{FALSE}, the x-axis labels are hidden.}
\item{showYtickLabels}{Logical. Default: \code{TRUE}. If set to
\code{FALSE}, the y-axis labels are hidden.}
\item{xLabelPositions}{Either \code{"auto"} or a vector of values. The positions of
labels on the x-axis. If \code{"auto"}, the placement of labels is automatically
calculated.}
\item{yLabelPositions}{Either \code{"auto"} or a vector of values. The positions of
labels on the y-axis. If \code{"auto"}, the placement of labels is automatically
calculated.}
\item{fontSizes}{A vector of length 5. The first number is the size of the
title. The second number is the size of the axes titles. The third number
is the size of the axes values. The fourth number is the size of the
legends' titles. The fifth number is the font size of the legend labels.}
\item{colours}{The colours to plot data of each class in. The length of this
vector must be as long as the distinct number of classes in the data set.}
\item{showAssayName}{Logical. Default: \code{TRUE}. If \code{TRUE} and the
data is in a \code{MultiAssayExperiment} object, the the name of the table
in which the feature is stored in is added to the plot title.}
\item{classesColumn}{If \code{measurementsTrain} is a \code{MultiAssayExperiment}, the
names of the class column in the table extracted by \code{colData(multiAssayExperiment)}
that contains each sample's outcome to use for prediction.}
}
\value{
Plots are created on the current graphics device and a list of plot
objects is invisibly returned. The classes of the plot object are determined
based on the type of data plotted and the number of plots per feature
generated. If the plotted variable is discrete or if the variable is numeric
and one plot type was specified, the list element is an object of class
\code{ggplot}. Otherwise, if the variable is numeric and both the density
and stripchart plot types were made, the list element is an object of class
\code{TableGrob}.
Settling \code{lineWidth} and \code{dotBinWidth} to the same value doesn't
result in the density plot and the strip chart having elements of the same
size. Some manual experimentation is required to get similarly sized plot
elements.
}
\description{
Allows the visualisation of measurements in the data set. If \code{useFeatures}
is of type \code{\link{Pairs}}, then a parallel plot is automatically drawn.
If it's a single categorical variable, then a bar chart is automatically
drawn.
}
\examples{
# First 25 samples and first 5 genes are mixtures of two normals. Last 25 samples are
# one normal.
genesMatrix <- sapply(1:15, function(geneColumn) c(rnorm(5, 5, 1)))
genesMatrix <- cbind(genesMatrix, sapply(1:10, function(geneColumn) c(rnorm(5, 15, 1))))
genesMatrix <- cbind(genesMatrix, sapply(1:25, function(geneColumn) c(rnorm(5, 9, 2))))
genesMatrix <- rbind(genesMatrix, sapply(1:50, function(geneColumn) rnorm(95, 9, 3)))
genesMatrix <- t(genesMatrix)
rownames(genesMatrix) <- paste("Sample", 1:50)
colnames(genesMatrix) <- paste("Gene", 1:100)
classes <- factor(rep(c("Poor", "Good"), each = 25), levels = c("Good", "Poor"))
plotFeatureClasses(genesMatrix, classes, useFeatures = "Gene 4",
xAxisLabel = bquote(log[2]*'(expression)'), dotBinWidth = 0.5)
infectionResults <- c(rep(c("No", "Yes"), c(20, 5)), rep(c("No", "Yes"), c(5, 20)))
genders <- factor(rep(c("Male", "Female"), each = 10, length.out = 50))
clinicalData <- DataFrame(Gender = genders, Sugar = runif(50, 4, 10),
Infection = factor(infectionResults, levels = c("No", "Yes")),
row.names = rownames(genesMatrix))
plotFeatureClasses(clinicalData, classes, useFeatures = "Infection")
plotFeatureClasses(clinicalData, classes, useFeatures = "Infection", groupBy = "Gender")
genesMatrix <- t(genesMatrix) # MultiAssayExperiment needs features in rows.
dataContainer <- MultiAssayExperiment(list(RNA = genesMatrix),
colData = cbind(clinicalData, class = classes))
targetFeatures <- DataFrame(assay = "RNA", feature = "Gene 50")
plotFeatureClasses(dataContainer, useFeatures = targetFeatures, classesColumn = "class",
groupBy = c("clinical", "Gender"), # Table name, feature name.
xAxisLabel = bquote(log[2]*'(expression)'), dotBinWidth = 0.5)
}
\author{
Dario Strbenac
}
