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Wavelength vs. Absorbance Data Analysis

The document contains graphs and tables showing the relationship between wavelength, absorbance, and concentration for the compounds Bromothymol Blue and Thymol Blue. Specifically, it shows absorbance measurements across wavelengths from 350-700 nm for the individual compounds and their sum, and it shows increasing absorbance at 618 nm with higher concentrations of Bromothymol Blue. The data and graphs indicate the absorbance properties and behaviors of these compounds at different wavelengths and concentrations.

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meeechers
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107 views23,309 pages

Wavelength vs. Absorbance Data Analysis

The document contains graphs and tables showing the relationship between wavelength, absorbance, and concentration for the compounds Bromothymol Blue and Thymol Blue. Specifically, it shows absorbance measurements across wavelengths from 350-700 nm for the individual compounds and their sum, and it shows increasing absorbance at 618 nm with higher concentrations of Bromothymol Blue. The data and graphs indicate the absorbance properties and behaviors of these compounds at different wavelengths and concentrations.

Uploaded by

meeechers
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
Available Formats
Download as XLSX, PDF, TXT or read online on Scribd

Wavelength vs.

Absorbance
1
0.9
0.8
0.7
0.6
Absorbance (Bromothyl Blue)
Absorbace

Absorbance (Thymol Blue)

0.5
0.4
0.3
0.2
0.1
0
350

400

450

500

550

Wavelength (nm)

600

650

700

Wavelength vs. Absorbance (Sum of Bromothymol Blue and Thymol Blue)


1.2

0.8

Absorbance

Absorbance (Sum of Bromothymol Blue


and Thymol Blue)

0.6

0.4

0.2

0
350

400

450

500

550

Wavelength (nm)

600

650

700

Wavelength vs. Absorbance (Bromothyl Blue)


1
0.9
0.8
0.7
0.6

Absorbance

Absorbance (Bromothyl Blue)

0.5
0.4
0.3
0.2
0.1
0
350

400

450

500

550

Wavelengths (nm)

600

650

700

Wavelegth vs. Absorbance (Thymol Blue)


1
0.9
0.8
0.7
0.6

Absorbance

Absorbance (Thymol Blue)

0.5
0.4
0.3
0.2
0.1
0
350

400

450

500

550

Wavelength (nm)

600

650

700

Absorbance of Bromothymol Blue at 618 nm vs. Concentration


0.7

f(x) = 0.0274249268x

0.6

0.5

0.4

Absorbance
Linear (Absorbance)

Absorbance
0.3

0.2

0.1

0
0

10

15

Concentration in M

20

25

Absorbance vs. Wavelength


1.1
1
0.9
0.8
0.7
Absorbance (Bromothyl Blue)
Absorbance (Thymol Blue)

0.6
Absorbance

Absorbance (Sum of Bromothymol Blue


and Thymol Blue)

0.5
0.4
0.3
0.2
0.1
0
350

400

450

500

550

Wavelength (nm)

600

650

700

Wavelengt Absorbance AbsorbanceAbsorbance (Sum of BConcentration


Absorbance
in mol/L of the Brothymol Blue
370
0.15
0.551
0.701
23.2
0.651
380
0.174
0.606
0.78
18.6
0.459
390
0.191
0.665
0.856
13.9
0.396
400
0.195
0.731
0.926
9.3
0.298
410
0.183
0.799
0.982
4.64
0.128
420
0.16
0.845
1.005
430
0.139
0.872
1.011
434
0.133
0.874
1.007
440
0.12
0.87
0.99
450
0.108
0.821
0.929
460
0.103
0.75
0.853
470
0.104
0.657
0.761
480
0.105
0.571
0.676
490
0.111
0.471
0.582
500
0.123
0.374
0.497
510
0.14
0.299
0.439
520
0.16
0.245
0.405
530
0.188
0.2
0.388
540
0.224
0.172
0.396
550
0.27
0.159
0.429
560
0.318
0.159
0.477
570
0.37
0.167
0.537
580
0.422
0.179
0.601
590
0.487
0.196
0.683
600
0.55
0.195
0.745
610
0.597
0.171
0.768
618
0.603
0.143
0.746
620
0.6
0.131
0.731
630
0.545
0.092
0.637
640
0.437
0.056
0.493
650
0.302
0.031
0.333
660
0.189
0.016
0.205
670
0.103
0.008
0.111

8.604

13.076

Wavelengt Absorbance (Bromothyl Blue)


370
0.15
380
0.174
390
0.191
400
0.195
410
0.183
420
0.16
430
0.139
434
0.133
440
0.12
450
0.108
460
0.103
470
0.104
480
0.105
490
0.111
500
0.123
510
0.14
520
0.16
530
0.188
540
0.224
550
0.27
560
0.318
570
0.37
580
0.422
590
0.487
600
0.55
610
0.597
618
0.603
620
0.6
630
0.545
640
0.437
650
0.302
660
0.189
670
0.103

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