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DNASeq Process

The document describes the process for DNA sequencing. It involves mapping clones that span the genome region to be sequenced, creating a library of smaller clones from the mapped clones, purifying and preparing the template DNA from the smaller clones, performing sequencing chemistries to determine the sequences, using techniques to produce high quality sequences, and performing quality assurance, verification, biological annotation before submission to a public database.

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Sk. Salahuddin Ahammad
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63 views1 page

DNASeq Process

The document describes the process for DNA sequencing. It involves mapping clones that span the genome region to be sequenced, creating a library of smaller clones from the mapped clones, purifying and preparing the template DNA from the smaller clones, performing sequencing chemistries to determine the sequences, using techniques to produce high quality sequences, and performing quality assurance, verification, biological annotation before submission to a public database.

Uploaded by

Sk. Salahuddin Ahammad
Copyright
© Attribution Non-Commercial (BY-NC)
We take content rights seriously. If you suspect this is your content, claim it here.
Available Formats
Download as PDF, TXT or read online on Scribd

DNA Sequencing

Process

— Identity set of clones


that span region of genome Mapping
to be sequenced

— Make sets of smaller clones


from mapped clones Library
Creation

— Purify DNA from


smaller clones Template
— Set up and perform Preparation
sequencing
chemistries

— Determine
sequences from Gel
smaller clones Electrophoresis

— Specialty techniques
to produce high Pre-finishing
quality sequences
and Finishing

— Quality assurance
— Verification Data Editing/
— Biological annotation
— Submission to public database Annotation

JGI
JOINT GENOME INSTITUTE

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