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HPTLC Method for Paracetamol & Dexibuprofen

The document describes the development of a stability-indicating HPTLC method for the simultaneous estimation of paracetamol and dexibuprofen in bulk and pharmaceutical dosage forms. The method utilizes a silica gel plate and a mobile phase of n-hexane, ethyl acetate and glacial acetic acid. The method was validated according to ICH guidelines and showed good linearity and separation of degradation products from the pure drugs when subjected to forced degradation studies involving acid, alkali, oxidation and dry heat.

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Hemraj Soni
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67 views1 page

HPTLC Method for Paracetamol & Dexibuprofen

The document describes the development of a stability-indicating HPTLC method for the simultaneous estimation of paracetamol and dexibuprofen in bulk and pharmaceutical dosage forms. The method utilizes a silica gel plate and a mobile phase of n-hexane, ethyl acetate and glacial acetic acid. The method was validated according to ICH guidelines and showed good linearity and separation of degradation products from the pure drugs when subjected to forced degradation studies involving acid, alkali, oxidation and dry heat.

Uploaded by

Hemraj Soni
Copyright
© Attribution Non-Commercial (BY-NC)
We take content rights seriously. If you suspect this is your content, claim it here.
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Download as DOC, PDF, TXT or read online on Scribd

Journal of Chemical and pharmaceutical Sciences-Accepted

DEVELOPMENT OF STABILITY INDICATING HPTLC METHOD FOR


SIMULTANEOUS ESTIMATION OF PARACETAMOL AND DEXIBUPROFEN

ABSTRACT

The present work describes a stability-indicating HPTLC method for analysis of

paracetamol and dexibuprofen in bulk and pharmaceutical dosage form. Precoated silica

gel 60 F254 plate was used as stationary phase. The separation was carried out using n-

hexane: ethyl acetate: glacial acetic acid (5: 5: 0.2 v/v/v) as mobile phase. The

densitometric scanning was carried out at 223 nm. The linearity was obtained in the range

10–50 µg/band for paracetamol and 6-30 µg/band for dexibuprofen with correlation

coefficients (r2) 0.9915 and 0.9969 for paracetamol and dexibuprofen respectively. The

method was validated as per ICH guidelines. The combination was subjected to forced

degradation by acid, alkali, oxidation and dry heat. The degradation products were well

resolved from the pure drug with significantly different Rf values.

Keywords: Paracetamol; Dexibuprofen; HPTLC; Validation; Stability Studies.

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