Lyse Any Tough or Frozen Sample in 40 Seconds or Less!

FastPrep System

Only One with Interchangeable

Adapters
Optimized Lysing Matrix Tubes
and Complete Purification Kits
for Any Sample Available

The Most Advanced, Rapid and

Thorough Sample Lysis and
Extraction of DNA,
RNA and Proteins

www.mpbio.com
MP Biomedicals North America, Tel: 1.800.854.0530 MP Biomedicals Europe, Tel: 00800 7777 9999
MP Biomedicals Japan, Tel: 03.3808.2102 MP Biomedicals Singapore, Tel: 65.6775.0008 MP Biomedicals Australia, Tel: 02.9838.7422

MP Biomedicals

FastPrep-24 Instrument
Ultra-Rapid, Thorough Homogenization
Lyse Any Tough or Frozen Sample In 40 Seconds

sample material. FastPrep will

homogenize up to 24 samples in
2ml tubes, or with optional adapters,
lyse 48 samples in 2ml tubes, 12
samples in 15ml tubes or 2 samples
in 50ml tubes making FastPrep the
most versatile homogenizer available!

he new FastPrep-24 from MP

Biomedicals now features a
programmable memory of up to
five of your most-used settings.
The most recent evolution of the
famous high-throughput sample
preparation system from Bio101
Systems, with a total of more than
6,000 installations worldwide,
FastPrep provides a unique means
of homogenizing by which any
type of sample, no matter how
tough, can be quickly and consistently lysed within 40 seconds.
When compared to traditional
homogenization methods such
as vortexing, syringe shearing,
grinding with a mortar and pestle
or hammering samples that have
been frozen in liquid nitrogen, the
FastPrep-24 homogenizer will
save hours of work during the
sample preparation stage and
will provide higher yields of intact
DNA, RNA and proteins.
FastPrep-24 uses a unique,
optimized motion to disrupt cells
through the multidirectional,
simultaneous beating of specialized Lysing Matrix beads on the


Developed for difficult and resistant

samples, FastPrep-24 thoroughly
and quickly lyses all tissues and
cells providing easy and reproducible isolation of stable RNA, active
proteins and full-length genomic
DNA.
A completely self-contained system,
FastPrep-24 eliminates the risk of
cross-contamination and timeconsuming clean-up associated with
manual lysis methods. Simply add
sample and buffers to the Lysing
Matrix tube containing specialized
beads specific to your application.
The ergonomic design of FastPrep
ensures ease in loading sample
tubes, which remain securely sealed
during processing. Homogenization speed and duration times are
digitally controlled for consistent
results. Upon setting the speed and
time with the touch of the a button,
simply select this as a memory setting or push run, and in less than a
minute your samples are completely
lysed! The new FastPrep-24
features up to 5 memory presets for
your most-used settings.
The unique, vertical angular motion
of FastPrep-24 causes the
lysing matrix particles to impact the
sample from all directions simultaneously, releasing nucleic acids and
proteins into the protective buffer
faster than with any other system.

www.mpbio.com

Genomic DNA from human ovarian

tissue lysed with the FastPrep-24
for 20 sec. Courtesy of Dr. David
Smith, Oncotech Inc.
After centrifugation, the supernatant is collected for further purification processing.
A wide variety of specialized
Lysing Matrix tubes containing
beads of different materials, sizes
and shapes have been tailored
to guarantee thorough homogenization of samples from such
diverse sources as bacteria, yeast,
fungi, botanical samples (including seeds), insects, mammalian
tissues, (bone, skin, brain, tumor)
and cultured cells.
High performance FastPrep
Purification Kits, when used in
conjunction with the FastPrep-24
Instrument, provide simple, readyto-use methods for the release
and subsequent purification of
intact DNA, RNA, and proteins
from virtually any source.
Visit www.mpbio.com for additional
information and a demonstration.

MP Biomedicals

FastPrep-24
One Source for All Your Sample Preparation
Most Advanced, Rapid and Thorough Extraction of DNA, RNA and Proteins
Overview of
the FastPrep-24 Procedure

Homogenize resistant samples with ease

Only system with interchangeable adapters allows you to process
24x2ml; 48x2ml; 12x15ml; or 2x50ml samples
High reproducibility with precise setting of lysis time and speed.
Now available with 5 pre-set memory

Cells and lysing buffer

Eliminate cross contamination with single-use Matrix tubes

Complete sample preparation for extraction and purification of DNA,
RNA and Proteins with FastPrep Kits

1. Prepare lysis tube

EFFICIENTLY LYSE:
4Bone, brain, tumors
4Bacteria gram + or
4Yeast, fungi, spores
4Seeds,roots
4Feces, soil

40 seconds

2. Process with FastPrep-24 Instrument

1 minute

3. Centrifuge to pellet debris

Gel
analysis

DNA
Protein
RNA

N E W EASY-TO-USE PROGRAMMABLE INSTRUMENT!

Save up to 5 pre-sets that you use most,
with specific combinations of speed and time.

Purify

Cat. #
4. Transfer cleared lysate

6003-500

Description
FastPrep -24 Instrument

www.mpbio.com

Price
$ 9,990.00


MP Biomedicals

FastPrep-24 is the ONLY Homogenizer with

Interchangeable Adapters!
QuickPrepTM Adapter

New

24 x 2 ml samples
(included with FastPrep-24 Instrument)

CryoPrep TM Adapter

TeenPrepTM Adapter

24 x 2 ml samples
under temperature-controlled conditions

12 x 15ml samples

BigPrepTM Adapter

HiPrepTM Adapter

2 x 50 ml samples

48 x 2ml samples

The ergonomic design of FastPrep-24 optional adapters ensures easy

loading of Lysing Matrix tubes that remain securely sealed during
homogenization. All the adapters stand stable on the benchtop and
are commonly used as tube racks for sample storage at -20C or
-80C. Frozen Lysing Matrix tubes loaded in the adapters are ready
to be immediately processed in the FastPrep-24 with minimal
hands-on manipulation, and the new CryoPrep adapter keeps frozen
samples chilled to -800C.

Cat. #

Description

6002-512

QuickPrep Adapter

6002-525

BigPrep Adapter

6002-526

TeenPrep Adapter

6002-527

HiPrep Adapter

6002-528

CryoPrep Adapter

www.mpbio.com

MP Biomedicals

Coolest Sample Preparation Adapter in the World

CryoPrep

24 x 2ml Tubes Adapter equipped with a Temperature-Controlling System

New COOL tool from
,
The Worlds Leader in Sample Preparation and Lysis

CryoPrep is a novel FastPrep-24 adapter, which allows simultaneous

cryogenic lysis of 24 x 2ml samples. Based on passive temperature control
technology, the CryoPrep adapter ensures an efficient cooling of the samples
as dry-ice, alcohol, water-ice or water placed into the moving tray are in
direct contact with the sample tubes. Due to a high heat transfer capacity and
Fastprep precise settings of lysis parameters, the samples can be repeatably
homogenized in their unthawed state. This new adapter is ideally suited for
extractions of any temperature unstable or sensitive biological compounds
such as RNA, siRNA, metabolites, intermediates, and enzymes from even the
hardest samples to lyse. Most samples can be processed in 40 seconds or
less.

ology with m
The FastPrep System: a proven techn

ore than 6,000 users worldwide

www.mpbio.com

Cat. #

Description

6002-528

CryoPrep Adapter

MP Biomedicals
Double the Value of Your Investment and Cut Your Time in Half with

Our New High Throughput (48 x 2ml), Double-Decker FastPrep Adapter

HiPrep

48 x 2ml Tubes Adapter

The New, Revolutionary Concept from the Worlds Leader in

Sample Preparation Technologies

New 48 x 2ml tubes adapter for FastPrep-24 instrument,

effectively doubles its capacity, and your throughput.

Ideally suited for high throughput and pharmacological

research on extracted DNA, RNA, proteins, enzymes, cell
components and small molecules/metabolites. Lyse a variety
of samples including cells, microorganisms, spores, soils,
sediments, feces, forensic samples, food samples, plant and
animal tissues, bones, polymers, inorganic materials,
pharmaceutical products and many more.

www.mpbio.com

Cat. #

Description

6002-527

HiPrep Adapter

MP Biomedicals
A Revolutionary Concept from the Leader of Sample Preparation Technologies:
Largest, Fastest and Cleanest Sample Preparation System

BigPrep
2 x 50ml Tubes Adapter

The New Large-scale Lysis Adapter for the FastPrep-24 Instrument

Simultaneously homogenize two large samples, up to 2 x 50 ml

Efficiently lyse in 40 seconds or less:
Cell cultures, bacteria, yeast, spores, animal and plant tissues,

bones, swabs, soil, sediments, feces, forensic samples, food

samples, polymers, etc

Ideally suited for:

DNA and RNA Isolation

Enzyme Isolation and Protein Production

Natural Products Isolation
Food Preparation for Safety and Quality Analysis
Biopharma Manufacturing
And many more

No cross-contamination
Easy to clean and decontaminate
Supersized proven FastPrep technology
Compatible with SafTest Food Testing System
Optimized ready-to-use 50 ml Lysing Matrix Tubes
for any sample available
Ergonomic loading system

ology with mo
The FastPrep System: a proven techn

www.mpbio.com

re than 6,000 users worldwide

Cat. #

Description

6002-525

BigPrep Adapter

MP Biomedicals
No Sample Can Resist Lysis and Homogenization in our 15 ml Fastprep Adapter

TeenPrep
12 x 15ml Tubes Adapter

Introducing Another Revolutionary Adapter from

The Worlds Leader In Sample Preparation Technologies

Efficiently lyse in 40 seconds or less:

Cell cultures, bacteria, yeast, spores, animal and plant tissues, bones, swabs, soil,
sediments, feces, forensic samples, food samples, polymers, etc
Ideally suited for:
RNA, Proteins, Enzymes Isolation; Natural Products Isolation; Environmental Science;
Forensic Science; Food Safety and Quality Analysis; Biopharma manufacturing and
many more
Lyse up to 12 samples in convenient 15ml centrifuge tubes
Reproducible and accurate lysis due to precise speed and time settings
Never worry about cross-contamination
Easy to clean and decontaminate
Compatible with SafTest Food Inspection System

Ergonomic loading system

www.mpbio.com

Cat. #

Description

6002-526

TeenPrep Adapter

MP Biomedicals

Convenient Lysing Matrix Tubes for Every Need

MP Biomedicals guarantees the BEST performance from your FastPrep-24 Instrument
when used in combination with FastPrep Lysing Matrix Tubes
Matrices are critical components of the FastPrep Sample
Preparation system.
Matrices are available separately for use with your own
unique buffers, and are also available as components of
the complete purification kits on the following pages.

Lysing Matrix A

Each impact-resistant 2 ml tube contains garnet

matrix and one 1/4 inch ceramic sphere. Extra
1/4 inch ceramic spheres are packaged separately. Lysing Matrix A tubes have orange caps and
are found in the FastDNA and FastDNA SPIN
Kits. Lysing Matrix A is used for all sample types
except soil for the subsequent isolation of
genomic DNA.

Lysing Matrix B
New Easy Dispenser Box for all Matrix Kits of 100 and 500 Tubes

Cat. #

Description

Pack size

6910-050

Lysing Matrix A

50 x 2 ml Tubes

6910-100

Lysing Matrix A

100 x 2 ml Tubes

6910-500

Lysing Matrix A

500 x 2 ml Tubes

6911-050

Lysing Matrix B

50 x 2 ml Tubes

6911-100

Lysing Matrix B

100 x 2 ml Tubes

6911-500

Lysing Matrix B

500 x 2 ml Tubes

6912-050

Lysing Matrix C

50 x 2 ml Tubes

6912-100

Lysing Matrix C

100 x 2 ml Tubes

6912-500

Lysing Matrix C

500 x 2 ml Tubes

6913-050

Lysing Matrix D

50 x 2 ml Tubes

6913-100

Lysing Matrix D

100 x 2 ml Tubes

6913-500

Lysing Matrix D

500 x 2 ml Tubes

6914-050

Lysing Matrix E

50 x 2 ml Tubes

6914-100

Lysing Matrix E

100 x 2 ml Tubes

6914-500

Lysing Matrix E

500 x 2 ml Tubes

6750-200

BioPulverizer System I
(10 tubes each of 6910-6914)

50 x 2 ml Tubes

www.mpbio.com

Each impact-resistant 2 ml tube contains 0.1 mm

silica spheres. Lysing Matrix B tubes have blue
caps and are found in the FastRNA Pro Blue Kit
and FastProtein Blue Matrix. Lysing Matrix B is
used for lysis of gram positive and gram negative
bacteria.

Lysing Matrix C

Each impact-resistant 2 ml tube contains 1 mm

silica spheres.Lysing Matrix C tubes have red
caps and are found in the FastRNA Pro Red Kit
and FastProtein Red Matrix. Lysing Matrix C is
used for lysis of yeast and fungi.

Lysing Matrix D

Each impact-resistant 2 ml tube contains 1.4 mm

ceramic spheres. Lysing Matrix D tubes have
green caps and are found in the FastRNA Pro
Green Kit for isolation of total RNA from plants
and animals.

Lysing Matrix E

Each impact-resistant 2 ml tube contains 1.4 ceramic spheres, 0.1 mm silica spheres, and one
4 mm glass bead. Lysing Matrix E tubes have
purple caps and found in the FastDNA SPIN Kit
for Soil and the FastRNAPro Soil Kits.


MP Biomedicals

FastPrep FP120 Instrument

50ml Lysing Matrix Tubes

The Proven Instrument for the Lysis

of up To 12 Samples in 2 ml Tubes

Cat. #

Description

Pack size

6950-010

BigA - Lysing Matrix Tubes

10 x 50ml Tubes

6950-050

BigA - Lysing Matrix Tubes

50 x 50ml Tubes

6951-010

BigB - Lysing Matrix Tubes

10 x 50ml Tubes

6951-050

BigB - Lysing Matrix Tubes

50 x 50ml Tubes

6953-010

BigD - Lysing Matrix Tubes

10 x 50ml Tubes

6953-050

BigD - Lysing Matrix Tubes

50 x 50ml Tubes

6960-010

BigClean - Lysing Matrix Tubes

with stainless steel beads

10 x 50ml Tubes

6960-050

BigClean - Lysing Matrix Tubes

with stainless steel beads

50 x 50ml Tubes

6954-010

Big E-Lysing Matrix Tubes

10 x 50ml Tubes

6954-050

Big E-Lysing Matrix Tubes

50 x 50ml Tubes

15ml Lysing Matrix Tubes

Cat. #

10

Description

The FastPrep120 Instrument

provides extremely rapid,
oscillating reciprocal motion
for efficient disruption of
cells and tissues.
It is the little brother to
our workhorse instrument,
FastPrep-24, and is limited
to 12 samples at time.

SPECIFICATIONS:
12 x 2ml samples homogenized simultaneously
Time:

1-40 sec. range, programmable in 1 sec. increments

Speed:

4.06.0 m/sec. range,

programmable in 0.5 m/sec. increments

Acceleration:

Less than 2 sec. to max. speed

Deceleration:

Less than 2 sec. from max. speed

Construction:

Anodized aluminum sample holder, polypropylene

sample retentionspoke plate, chemical-resistant
painted surfaces

Dimensions
(WxDxH):

28cm x 40cm x 30.5cm (11in. x 15.75in. x 12in.)

Weight:

16.3kg (36lb.)

Registration:

ISO 9001-1987. Registered by QMI

(Quality Management Institute).Certificate #001080.

Pack size

6930-050

TeenA - Lysing Matrix Tubes

50 x 15ml Tubes

6931-050

TeenB - Lysing Matrix Tubes

50 x 15ml Tubes

6932-050

TeenC - Lysing Matrix Tubes

50 x 15ml Tubes

6933-050

TeenD - Lysing Matrix Tubes

6934-050

TeenE - Lysing Matrix Tubes

Cat. #

Description

6001-120

FastPrep FP120 Instrument, US & Canada - 120V

50 x 15ml Tubes

6001-100

FastPrep FP120 Instrument, Japan - 100V

50 x 15ml Tubes

6001-220

FastPrep FP120 Instrument, Europe & Asia - 220V

www.mpbio.com

MP Biomedicals

Ready-to-use Protocols for DNA, RNA and Protein

Isolation from Any Sample!
A Wide Range of FastPrep Kits
Rapid and reproducible sample lysis and purification
process

Integrity and size of DNA, RNA and Proteins are

retained

No cross contamination with closed lysing matrix tubes

Increased yields of high quality DNA, RNA and Proteins

Nucleic acids and Proteins are ready-to-use in down

stream applications

FastProtein Blue Matrix

FastDNA Spin Kit for Soil

Cat N 6560-200 (50 preps)

Cat N 6550-400 (50 preps) - Cat N 6550-500 (100 preps)

Variety of soil and environmental sample types

No hazardous organic reagents required
SPIN filters streamline silica handling

Release of proteins from gram positive and gram

negative bacteria in 40 seconds
Protein extracts are ready for immediate electrophoresis or purification
Ideal for optimizing induction conditions

DNA
PROTEIN

FastPrep Kits

FastDNA Kit
FastDNA Spin Kit
Cat N 6540-400 - Cat N 6540-600 respectively

FastRNA Pro Green Kit

Cat N 6045-050 (50 preps)

For use with all plant and animal samples

Lyse 50-100 mg tissue per 2ml tube

(100 preps)

RNA
FastRNA Pro Red Kit
Cat N 6035-050 (50 preps)

Plant, animal, yeast, fungal and

microbial samples
No hazardous organic reagents
required
SPIN filters streamline silica handling
(FastDNA Spin Kit)

For use with yeast cells and fungal tissue

Lyse up to 1010 cells per 2ml tube

FastRNA Pro Soil-Direct Kit

FastRNA Pro Soil-Indirect Kit
Cat N 6070-050 - Cat N 6075-050 respectively (50 preps)

Variety of soil and environmental sample types

RNA protected during and after processing
Humic acids reduced to allow uninhibited RT-PCR
Includes additional reagents for even further purification
if necessary
SPIN filters streamline silica handling

FastRNA Pro Blue Kit

Cat N 6025-050 (50 preps)

For use with gram positive and gram

negative bacteria
Lyse up to 1010 cells per 2ml tube

www.mpbio.com

FastProtein Red Matrix

Cat N 6550-600 (50 preps) - Cat N 6550-700 (100 preps)

Release of proteins from yeast cells and fungi in

40 seconds
Protein extracts are ready for immediate electrophoresis or purification
Ideal for optimizing induction conditions

11

MP Biomedicals

FastDNA SPIN Kit

A Rapid Method of Isolating Pure Genomic DNA from a Wide Variety of Sources!
Rapid and reproducible sample lysis with the FastPrep-24 or FastPrep FP120
Isolate PCR-ready DNA from a variety of sample types
No hazardous organic reagents are required

The FastDNA SPIN Kit quickly and efficiently isolates genomic DNA from almost any sample (plant and animal tissues, cultured
cells, bacteria, yeast, fungi, insects, etc). Up to 200 mg of tissue or cells are processed by the FastPrep-24 or FastPrep FP120
with the Lysing Matrix A tubes. The kit includes 3 different chaotropic buffers for the homogenization of a wide variety of sample types
and the released DNA is purified by a silica-based spin filter method. Purified DNA is ready for enzyme digestion, electrophoresis,
PCR and any other desired application.
References
1. Hill J.E. et al (2005). Appl.Environ.Microbiol. Vol 71 : 867-875
2. Moon H. et al (2004). J.Exp.Bot. Vol 55 : 1519-1528
3. Dionisi H.M. et al (2004). Appl.Envir.Microbiol. Vol 70 : 3988-3995

Cat #

Designation

Pack Size

6540-600

FastDNA SPIN Kit

100 Preps

FastDNA SPIN Kit For Soil

Isolate Pure DNA from Cells present in Soil or other Environmental Samples!
Rapid and reproducible sample lysis with the FastPrep24 or FastPrep FP120 Instrument
Easily isolate DNA from a variety of organisms in many different types of soil
No hazardous organic reagents are required
The FastDNA SPIN Kit for Soil is designed to efficiently isolate bacterial, fungi, plant and animal genomic DNA from soil and environmental samples. Up to 500 mg soil are processed by the FastPrep-24 or FastPrep FP120 with the Lysing Matrix E tubes
designed to efficiently lyse all microorganisms including difficult sources such as eubacterial spores and endospores, gram positive
bacteria and yeast. The released DNA is purified by a silica-based spin filter method and is suitable for PCR analysis and other
downstream applications.
DNA from various soil samples extracted with the FastDNA SPIN Kit for Soil.
20% of the DNA isolated from 500mg soil was loaded on a 1.2% agarose gel (0.5X TAE).
Soil was taken from: Lane 1: tomato pot; Lane 2 : sludge;
Lane 3 : sandy soil; Lane 4 : under pine tree;
Lane 5 : under palm tree; Lane 6 : green garden;

Lane 7 : Nile Lilly pot; Lane 8 : lawn grass;

Lane 9 : citrus tree; Lane 10 : avocado tree. DNA ranges from 4-20 kb.

References
1. Selesi D. et al (2005). Appl. Envir. Microbiol. Vol 71 : 175-184
2. Alexandrino M. et al (2004). Water Research. Vol 38 : 1340 - 1346
3. Mumy K.L. et al (2004). J. of Microbiological Methods. Vol 57 : 259 268

12

Cat #

Designation

Pack Size

6560-200

FastDNA SPIN Kit for Soil

50 Preps

www.mpbio.com

MP Biomedicals

FastRNA Pro Kits

Isolate High Quality Total RNA with a Single-Reagent Extraction Method!
Rapid and reproducible sample lysis in under 40 seconds with the FastPrep24 or FastPrep FP120
Safe and consistent RNA isolation with the single-reagent RNAProTM solution
Lysis and purification of total RNA
The FastRNA Pro Kits are designed to quickly and efficiently isolate total RNA from virtually
any sample. During the homogenization step, intact total RNA is released in the proprietary
RNAProTM solution where it is immediately stabilized. The RNAProTM solution inactivates
cellular RNases during cell lysis to prevent RNA degradation. RNA is then extracted with
chloroform and precipitated with ethanol. DEPC-treated water is provided for resuspension
of total RNA. High quality RNA prepared with FastRNA Pro Kits is ready for all downstream
applications including RT-PCR, gene expression and microarray analysis.
Cat #

Designation

Pack Size

6025-050

FastRNA Pro Blue Kit (bacteria)

50 Preps

6035-050

FastRNA Pro Red Kit (yeast and fungi)

50 Preps

6045-050

FastRNA Pro Green Kit (plants andanimals)

50 Preps

Tail Kidney Liver Ear

0.249.5kb
NA
Brain R
Ladder

Rat total RNA extracted with the

FastRNA Pro Green Kit.
Approximatively 2% of the total RNA
isolated from 100 mg frozen tissue
was loaded on to a 1.2% denaturing
agarose gel (1X MOPS).

References
1. Vido K. et al (2005). J. Bacteriol. Vol 187 : 601-610
2. Tsai H.F. et al ( 2004). Antimicrob. Agents Chemoth. Vol 48 : 2483 2489
3. Tupin E. et al (2004). J. Exp. Med. Vol 199 : 417 -422

FastRNA Pro Soil Kits

Isolate Total RNA From Soil that is Immediately Ready for RT-PCR and other Downstream Applications!
Rapid and reproducible sample lysis in under 40 seconds with the FastPrep-24 or FastPrep FP120
Easily lyse difficult gram positive cells, plant material, and organic debris directly from soil
Lysis and purification solutions protect RNA during processing
Humic acids levels reduced to allow uninhibited RT-PCR
Lysis and purification of total RNA
The FastRNA Pro Soil-Direct and Indirect kits are designed to efficiently isolate total RNA from organic material found in soil
samples or soil supernatants.
The direct method consists of extracting nucleic acid from microorganisms and other biological specimens directly from soil.
The indirect method utilizes an initial separation of microorganisms and other biological specimens from the soil followed by lysis
of the organisms and RNA purification. This method also permits soil incubation with growth media in order to amplify underrepresented living organisms prior to RNA isolation if specific comparisons of microbial diversity are not desired.
FastRNA Pro Soil kits purify RNA in a process that removes humic substances and other inhibitors,and efficiently inactivates
cellular RNases during homogenization to prevent RNA degradation. Purified RNA is thus suitable for RT-PCR analysis and other
downstream applications.

Cat #

Designation

Pack Size

6070-050

FastRNA Pro Soil-Direct Kit

50 Preps

6075-050

FastRNA Pro Soil-Indirect Kit

50 Preps

www.mpbio.com

13

MP Biomedicals

Easy Lysis of Microorganisms to

Release Recombinant Proteins
FastProtein Matrix
Save time by reducing sample lysis time to seconds
Quickly and consistently lyse samples from different time points or induction conditions
Protein extract is ready for immediate electrophoresis or purification
Prepare dozens of protein samples in minutes!
The FastProtein products employ a powerful, patented technology for the rapid lysis of yeast and bacteria. Used
in conjunction with the FastPrep-24 or FastPrep FP120 Instrument, these products offer the fastest way to release
expressed proteins from the host organism.
FastProtein Kits are perfect for analyzing protein expression conditions using gel analysis. Samples are enclosed
during the quick lysis step, thus preventing cross-contamination or sample loss.
Bacteria

Yeast

FastProtein Blue

For Lysis Of Gram Positive

And Gram Negative Bacteria
The FastProtein Blue matrix is optimal for lysing
gram positive and gram negative bacteria. These fine
glass beads are designed for use with gram positive
bacteria or any difficult microorganism. Cells, resuspended in either 1X PBS or your own expression buffer, are added to the Lysing Matrix and processed in the
FastPrep-24 or FastPrep FP120 for 2040 seconds.
Figure 1
12% SDS PAGE of lysate of
BL21 cells expressing the GST
protein resulting from homogenization with the FastProtein
Blue Matrix

Figure 2
12% SDS PAGE of lysate
of yeast cells resulting from
homogenization with
FastProtein Red .

FastProtein Red
Cat. #

Description

Size

6550-400

FastProtein Blue Matrix

50 x 2ml

6550-500

FastProtein Blue Matrix

100 x 2ml

6550-600

FastProteinRed Matrix

50 x 2ml

6550-700

FastProteinRed Matrix

100 x 2ml

14

For Lysis Of Yeast Cells

The FastProtein Red matrix is used to lyse yeast cells. Cells,
resuspended in either Yeast Breaking Buffer (YBB-supplied
with the kit) or your own expression buffer, are added to the
small glass beads of this Lysing Matrix and processed in
the FastPrep-24 or FastPrep FP120 Instrument for 2040
seconds.

www.mpbio.com

MP Biomedicals

Table of Typical FastPrep Systems Settings for Optimal Sample Lysis

Sample Name

Sample Type

Quantity

Lysing Matrix

FastPrep speed

FastPrep time

HUMAN AND ANIMAL

Human

Lung

50 mg

Lysing Matrix D

6.0

4x 30 sec.

Human

Breast

80 mg

Lysing Matrix D

6.0

2x 30 sec.

Human

Kidney

50 mg

Lysing Matrix D

6.0

40 sec.

Human

Thyroid Tumors

100 mg

Lysing Matrix A

6.0

3x 30 sec.

Mouse

Eye

10 mg

Lysing Matrix D

6.0

4x 30 sec.

Mouse

Heart

70 mg

Lysing Matrix D

6.0

4x 30 sec.

Mouse

Kidney

50 mg

Lysing Matrix D

6.0

40 sec.

Mouse

Femur

40 mg

Lysing Matrix A

6.0

4x 30 sec.

Mouse

Leg Muscle

50 mg

Lysing Matrix D

6.0

40 sec.

Mouse

Intestine

50 mg

Lysing Matrix D

6.0

40 sec.

Mouse

Ear

45 mg

Lysing Matrix D

6.0

4x 30 sec.

Mouse

Tail

100 mg

Lysing Matrix A

6.0

4x 30 sec.

Mouse

Spleen

70 mg

Lysing Matrix D

6.0

40 sec.

Mouse

Lung

50 mg

Lysing Matrix D

6.0

40 sec.

Mouse

Liver

50 mg

Lysing Matrix D

6.0

40 sec.

Mouse

Brain

50 mg

Lysing Matrix D

6.0

40 sec.

Mouse

Pancreatic cells (bHC9)

107 cells

Lysing Matrix D

6.0

40 sec.

PLANT
Alpowa Wheat

Leaf Tissue

75 mg

Lysing Matrix D

6.0

40 sec.

Alpowa Wheat

Seed

100 mg

Lysing Matrix A

6.0

40 sec.

Arabidopsis thaliana

Fresh Leaves

50 mg

Lysing Matrix D

6.0

40 sec.

Arabidopsis thaliana

Fresh Leaves

200 mg

Lysing Matrix D

6.0

2x 40 sec.

Bartlett Pear

Leaf Tissue

50 mg

Lysing Matrix D

6.0

40 sec.

Classic Oat

Leaf Tissue

75 mg

Lysing Matrix D

6.0

40 sec.

Classic Oat

Seed

100 mg

Lysing Matrix A

6.0

40 sec.

Corn

Leaf Tissue

100 mg

Lysing Matrix D

6.0

40 sec.

Crest Barley

Leaf Tissue

100 mg

Lysing Matrix D

6.0

40 sec.

Crest Barley

Root

300 mg

Lysing Matrix A

6.0

40 sec.

Kaybonnet Rice

Leaf Tissue

100 mg

Lysing Matrix D

6.0

40 sec.

Kaybonnet Rice

Seed

100 mg

Lysing Matrix A

6.0

40 sec.

Klages Barley

Root

300 mg

Lysing Matrix A

6.0

40 sec.

Klages Barley 70 mg Leaf

Tissue 6.0 40 seconds

Leaf Tissue

70 mg

Lysing Matrix D

6.0

40 sec.

Tobacco

Leaf Tissue

75 mg

Lysing Matrix D

6.0

40 sec.

www.mpbio.com

15

MP Biomedicals
Table of Typical FastPrep Systems Settings (continued)
Sample Name

Sample Type

Quantity

Lysing Matrix

FastPrep speed

FastPrep time

Lafitte Rice

Leaf Tissue

75 mg

Lysing Matrix D

6.0

40 sec.

Lafitte Rice
Soybean

Sprout Leaf

100 mg

Lysing Matrix D

6.0

2x 30 sec.

Seed

100 mg

Lysing Matrix A

6.0

40 sec.

Corn

Seed

100 mg

Lysing Matrix A

6.0

40 sec.

Oat FL 502

Leaf Tissue

75 mg

Lysing Matrix D

6.0

40 sec.

Oat FL 502

Seed

100 mg

Lysing Matrix A

6.0

40 sec.

PLANT

Riser Oat

Leaf Tissue

70 mg

Lysing Matrix D

6.0

40 sec.

Richland Soybean

Leaf Tissue

100 mg

Lysing Matrix D

6.0

40 sec.

Tam Wheat

Leaf Tissue

75 mg

Lysing Matrix D

6.0

40 sec.

Tam Wheat

Root

80 mg

Lysing Matrix A

6.0

40 sec.

Tomato, Early Girl

Leaf Tissue

75 mg

Lysing Matrix D

6.0

4 x 30 sec.

Williams 82 Soybean

Leaf Tissue

70 mg

Lysing Matrix D

6.0

40 sec.

Wrens Rye

Leaf Tissue

100 mg

Lysing Matrix D

6.0

40 sec.

Pine

Needle

100 mg

Lysing Matrix A

6.0

30 sec.

BACTERIA
Listeria monocytogenes

Cells

10 cells

Lysing Matrix B

6.0

3x 30 sec.

Streptococcus pyogenes

Cells

109 cells

Lysing Matrix B

6.0

20 sec.

Streptococcus mutans

Cells

10 cells

Lysing Matrix B

6.0

30 sec.

Staphylococcus aureus

Cells

10 cells

Lysing Matrix B

6.0

2x 40 sec.

Photorhabdus luminescens

Cells

109 cells

Lysing Matrix B

6.0

2x 30 sec.

Escherischia coli

Cells

10 cells

Lysing Matrix B

6.0

30 sec.

Mycobacterium tuberculosis

Cells

108 cells

Lysing Matrix B

6.0

2x 45 sec.

Lactococcus lactis

Cells

108 cells

Lysing Matrix B

6.0

3x 30 sec.

40 sec.

YEAST AND FUNGI

Saccharomyces cerevisiae

Cells

2x 108 cells

Lysing Matrix C

6.0

Schizosaccharomyces pombe

Cells

10 cells

Lysing Matrix C

5.0

4x 15 sec.

Candida albicans

Cells

108 cells

Lysing Matrix C

6.0

2x 30 sec.

Cryptococcus neoformans

Cells

108 cells

Lysing Matrix C

6.0

4x 30 sec.

Aspergillus fumigatus

Cells

108 cells

Lysing Matrix C

6.0

2x 30 sec.

Fusarium solani

Cells

10 cells

Lysing Matrix C

6.0

2x 30 sec.

ENVIRONMENTAL SAMPLES

16

Sediments

Soil/rocks

50 mg

Lysing Matrix E

5.5

2x 30 sec.

Soil

Sandy sample

50 mg

Lysing Matrix E

4.0

4x 30 sec.

Soil

Litter

50 mg

Lysing Matrix E

5.5

30 sec.

Feces

Turd

300 mg

Lysing Matrix E

6.0

40 sec.

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MP Biomedicals

Example of Onco-Pathology Related Applications of FastPrep-24

Sample Preparation of Biopsy Tissues for Genomic Analysis and
Drug Resistance Screening
Human-derived biopsy specimens of primary and secondary tumors are
usually complex matrices which are very hard to properly homogenize using
the classical methods. Their mechanical consistencies vary widely. The FastPrep-24 System, with its unique disruption mechanism and accurate settings,
allows for rapid, repeatable and reliable sample lysis and homogenization,
and produces highest quality of functional genomic DNA, RNA and proteins
for a variety of research, diagnostics and pharmacology applications.

A 1 2

7 8

10 11 12 13 14 15 16

17 18 19 20 21 22

23 24

B 1 2

7 8

10 11 12 13 14 15 16

17 18 19 20 21 22

23 24

B 1 2

7 8

10 11 12 13 14 15 16

17 18 19 20 21 22

23 24

B 1 2

7 8

10 11 12 13 14 15 16

Row 4

Row 3

Row 2

Row 1

As an example of high-throughput screening, here are 85 genomic DNAs isolated from human ovary cancer tumor
biopsies via the FastPrep-24, and automatically purified via the BioMEK3K robot. FastPrep prepared lysate is directly
compatible with third party high-throughput automation and automation kits

FastPrep-24 settings: 6.0 m/s for 40 seconds (one pass), using lysing matrix A

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17

MP Biomedicals

Sequencing of FastPrep Isolated DNA

The FastPrep-Isolated DNA is Ready for High-Throughput Sequencing

An example of the sequencing analysis of genomic DNA extracted using the FastPrep-24 system from a single biopsy melanoma tissue. The target wild type sequence is GTGACA with a documented mutation at T (usually a tranversion to A).
Note that the first panel is wild type, second panel is a mixture of wild type and mutant and the third panel is a pure mutant.
Clear evidence for heterogeneity within a single biopsy specimen. The sequencing gels further demonstrate high quality of DNA
extracted from FastPrep systems generated lysates

9 10 11 12 13 14 15 16

Gel of gDNA isolated from melanoma specimens

18

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MP Biomedicals

RNA Extraction From Basil Leaves

1

9
Line 1. Marker
Line 2. 1x40sec. with FastPrep FP120 Instrument
Line 3. 2x40sec. with FastPrep FP120 Instrument
Line 4. 3x40sec.with FastPrep FP120 Instrument
Line 5. 1x40sec. with FastPrep 24 Instrument
Line 6. 2x40sec. with FastPrep 24 Instrument
Line 7. 3x40sec. with FastPrep 24 Instrument
Line 8. 1x40sec. with FastPrep FP120 Instrument
Line 9. 1x40sec. with FastPrep 24 Instrument

50mg of basil leaves were homogenized with the FastPrep-24 or FastPrep FP120 Instrument. One, two or three runs of 40 seconds were
performed at a speed setting of 6.0 m/s and RNA was extracted with the FastRNA Pro Green Kit.

An Outstanding Rapid Method for the Isolation of

Pure Genomic DNA!
DNA isolation with the FastDNA SPIN kit
1. Add protective extraction buffer to Lysing Matrix Tube
2. Add sample and place in the FastPrep-24 Instrument

+B

+B

3. Process for 30-40 seconds, remove

4. Centrifuge to pellet debris
5. Mix clear supernatant with GLASSMILK (patented silica matrix)
6. Process through spin filter, wash and elute DNA
Eluted DNA is ready for enzyme digestion, electrophoresis, PCR and any other
downstream application. The FastDNA SPIN kit provides all the reagents required
for the purification process including the lysing matrix tubes.
200 mg of plant leaves and mouse tails were homogenized in the FastPrep-24
instrument for 40 seconds at a speed setting of 6.0 m/s. The FastPrep-24 instrument
uses a unique, optimized motion to homogenize samples by multidirectional, simultaneous impaction with lysing matrix particles.
After genomic DNA isolation according to the FastDNA SPIN Kit protocol, DNA
samples were analyzed by agarose gel electrophoresis (0.8% agarose gel stained
with ethidium bromide).

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Line 1: Marker (1Kb DNA ladder)

Line 2 & 3: Soft plant leaf
Line 4 & 5: Thick, fibrous plant leaf
Line 6 & 7: Mouse tail
Line 8: Marker
(DNA/HindIII fragments ladder)

19

MP Biomedicals

RNA and SiRNA Isolation from Cassava Roots

Using the FastPrep-24 System
Cassava root (Manihot esculenta), a plant native of South America, is a major annual crop in tropical and subtropical
regions. Its starchy tuberous root is a one of major sources of carbohydrates for human consumption, especially in Africa.
Due to root complex matrix structure which results in strong and starchy mechanical properties sample homogenization
and lysis of Cassava with classical methods is a cumbersome and unreliable process with small yield of RNA. Herein we
demonstate successful lysis and RNA extraction from Cassava roots and leaves in only 60 seconds using the
FastPrep-24 system and associated matrices.
RNA was extracted from Cassava roots after homogenization with the
FastPrep-24 Instrument and Lysing Matrix A tubes containing 2 ceramic
1/4 beads

Cassava roots

RNA extraction from Cassava storage roots;

samples contain 0.32g/l-110g/l RNA
FastPrep settings: Speed 6.0 for 60s
Lysing Matrix A with additional ceramic bead

Cassava Leaves
siRNA gel
RNA was extracted from Cassava leaves after homogenization with the
FastPrep-24 and Lysing Matrix A tubes. Total RNA was further processed for
siRNA isolation using the gradient separation procedure

siRNA extraction from Cassava leaves;

Samples contain 1.5g/l-2.5g/l RNA
FastPrep settings: Speed 6.0 for 30s
Lysing Matrix A

20

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???

MP Biomedicals

FastPrep-24 Applications
Questions and Answers

QA
&

Is it possible to isolate virus particles from animal

tissues with the FastPrep-24 System?

Is it possible to isolate RNA from paraffin-embedded

tissues with the FastPrep-24 System?

The FastPrep-24 System is designed for the isolation of viruses from animal and plant tissues. Researchers using the FastPrep-24 instrument for
this application reported that sample homogenization with Lysing Matrix A
tubes (garnet sand and one ceramic bead) induce lysis of viruses together
with the animal cells. When the garnet sand is removed and the homogenization process is performed with one ceramic bead, the animal cells
are lysed after one run of 20 sec. at speed 6.5 m/s but not the viruses,
meaning that the protein capsid is intact.
Reference: Klempa B. et al. (2005) J.Clin.Microbiol. Vol 43(6);
2756-2763.

Tissue samples to be used in microscopic and histological analy-

Are there specific recommendations to prevent RNA

degradation by RNases when isolating RNA with the
FastRNA Pro Kits?

ses are often preserved by embedding in paraffin. However, the

presence of paraffin may interfere with isolation of RNA. For isolation
of RNA from paraffin-embedded tissues, we recommend removing the
paraffin by xylene extraction before proceeding with the
FastRNA Pro procedure.
Materials:
- Xylene
- Ethanol (100%, 95%, 70% solutions)
- Distilled water
- Glass jars or other solvent containers (such as Coplin staining dish)
Procedure:
1. Section paraffin blocks at 5-10 microns

The origin of RNA degradation is often action of RNases: both

endogenous and exogenous RNases.

2. Place sections in a water bath at 42 C to and eliminate any folds

and wrinkles

1. Inactivation of endogenous RNases

3. Mount sections onto glass slides and let air dry overnight at

room temperature. If sections do not adhere to the slides, they

can be incubated at 42 C for up to 8 hours.

Endogenous RNases are released from cellular compartments immediately

after harvesting tissue and cells. It is essential to inactivate these RNases
as soon as possible to prevent RNA degradation.
To effectively inactivate endogenous RNases, add RNApro Solution (chaotropic-based cell lysis solution containing guanidium isothiocyanate) to
each sample as soon as possible following sample harvest and homogenize immediately with the FastPrep-24 instrument or flash-freeze samples
in liquid nitrogen. To prevent RNA degradation, it is important that the tissue be cut in small enough (1cm) pieces to allow rapid, thorough freezing
of the entire tissue.

4. Immerse the slides containing the tissue sections in solvents and

solutions as follows:
Xylene 5 minutes
Xylene 5 minutes
Ethanol, 100% 30 seconds
Ethanol, 95% 30 seconds
Ethanol, 70% 30 seconds,
dH2O 30 seconds

2. Reduce exposure to exogenous RNases

To isolate intact, high quality RNA, it is essential that exogenous
RNases are not introduced into purified RNA preparations. It is essential that any item that could contact the purified RNA is RNase-free. All
surfaces, including pipettors, benchtops, glassware and gel equipment, should be decontaminated with a surface decontamination
solution such as RNase Erase (Cat # 2440204). RNase-free tips, tubes,
and solutions should always be used and gloves should be changed frequently.

Which settings are recommended for a successful

homogenization of skin samples ?
20mg of full thickness skin samples are placed into Lysing Matrix A tubes containing 800l extraction buffer. Samples are homogenized with the FastPrep-24 instrument, 4 runs of 20 seconds are
performed at speed 6.0 m/s. Samples are incubated on ice for 2 minutes
between each run.

What are the settings for yeast lysis with the BigPrep
Adapter ?
The FastPrep-24 instrument in combination with the BigPrep Adapter has
been successfully used for the lysis of Pichia Pastoris, a yeast strain that
has a big cell wall. After centrifugation of 1 liter culture (5x 10e8 cells/ml),
the cell pellet is resuspended in 50 ml Lysis Buffer (containing protease
inhibitors and PMSF). 25ml of this solution is added to 2 Big Lysing Matrix
B tubes (containing silica beads) and samples are homogenized 4 times for
30 seconds at speed 6.0 m/s. Tubes were incubated on ice for 2 minutes
between each run. 80% of yeast cells were lysed.

Are Lysing Matrix tubes resistant to solvents and is it

possible to store them at -20C or -80C ?
Both tubes and lysing matrix beads are resistant to chemicals (acids,
bases, solvents). All Lysing Matrix tubes can be stored in freezers at
-20C and -80C

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21

MP Biomedicals

???

Is the FastPrep-24 System designed for the isolation of

enzymes? Is the enzymatic activity preserved?

Is the FastPrep-24 System designed for DNA

extraction from Cryptosporidium oocysts?

The FastPrep system is designed for the isolation of enzymes from any
sample. To preserve enzymatic activity, it is recommended to use Lysing
Matrix tubes with large beads like ceramic beads included in Lysing
Matrix A tubes and to homogenize samples for short times with incubation
on ice for at least 2 minutes between successive FastPrep homogenizations in order to prevent overheating of the sample.

There are three main problems associated with the isolation of DNA from
Cryptosporidium organisms: (i) the extreme robustness of the oocysts
(ii) the different physical and chemical nature of the matrices (faeces, water, food, soil) and their richness in PCR inhibitors
(iii) the low number of oocysts usually present in environmental samples.

GUS activity
(pmol MU-min-1 mg root-1)

Bao J.R. et al. (Can.J.Plant Pathol. (2002), Vol 24: 340-348) used the FastPrep System for the homogenization of tomato roots and isolation of GUS
enzyme and demonstrated that this method is yielding the highest enzymatic activity compared to other grinding methods:
Abstract: Insertion of beta-D-glucuronidase (GUS) reporter gene has been
found to be useful for detection, quantitation, and monitoring of plant-associated fungi in their environment. GUS was extracted from tomato roots
inoculated with a nonpathogenic strain of Fusarium oxysporum, 70T01,
that had been genetically modified to express both GUS activity and hygromycin B resistance. To facilitate studies of fungusplant interactions
using the GUS enzyme, we tested several methods for their efficiency of
preparation of fungal-encoded GUS from infected plant tissues, namely
FastPrep homogenization, grinding of tissues frozen in liquid nitrogen,
and extraction from lyophilized material. Of the three procedures, the FastPrep method yielded the highest GUS activity per unit of inoculated root
and provided twice the sensitivity of the other methods. This procedure
was also the easiest, quickest, and the most reliable. Up to 12 samples
could be analyzed in less than 2 h, and as little as 50 mg of fresh tissue was
sufficient. Of the factors examined that could affect extraction efficiency,
only the length of homogenization and the presence of protein stabilizers
(sucrose, bovine serum albumin, and protease inhibitors) in the GUS buffer
improved enzyme activity in the extracts. The FastPrep method was also
highly effective in enumerating fungal colony forming unit (CFU) populations in the root tissues, provided that the timing and speed of homogenization was controlled. Plating of infected root samples homogenized
using the FastPrep equipment and a mortar and pestle yielded about 50
times more CFUs per unit root than the colony counts obtained from whole
roots, dried and powdered roots, or lyophilized roots.

2
b

LN

In a recently published study from Jiang J. et al. (Appl. Environ. Microbiol.

(2005), Vol 71: 1135-1141), six DNA extraction methods for the detection of Cryptosporidium in water samples were evaluated. The authors
concluded that direct DNA extraction with the FastDNA SPIN kit for soil
in combination with the use of a high concentration of BSA represents
the most effective tool for PCR detection of Cryptosporidium oocysts in
water samples. This reduces the cost of current PCR detection of Cryptosporidium oocysts in water samples significantly as there is no need for
the expensive IMS of oocysts prior to DNA extraction. This method also
enables the use of extracted DNA for the analysis of other pathogens.

Is it possible to extract DNA from feces with the

FastPrep-24 System?
Several studies report the isolation of inhibitor-free DNA from feces with
the FastPrep System for analysis of their bacterial content.
Layton A. et al. (Appl.Environ.Microbiol. (2006), Vol 72: 4214-4224) and Ott
S.J. et al. (J.Clin.Microbiol. (2004), Vol 42: 2566-2572) extracted DNA from
human and animal feces with the FastDNA SPIN Kit for Soil for real-time
PCR assays and detection of bacterial species.
On the other side, Tannock G.W. et al. (Appl.Environ.Microbiol. (2000),
Vol 66: 2578-2588) and Requena T. et al. (Appl.Environ.Microbiol. (2002),
Vol 68:2420-2427) extracted DNA from 1ml human fecal sample with the
FastDNA Kit to monitor the composition of the fecal microflora by PCRDGGE

1
0

FP

Protocol for RNA extraction from murine corneas:

c
Control

Extraction method
Comparison of different extraction methods on fungal GUS enzyme activity recovered from tomato roots inoculated with a nonpathogenic strain of Fusarium oxysporum, 70T01, transformed with the GUS gene. Extraction methods
included FastPrep (FP), liquid nitrogen, (LN), and lyophilization and FastPrep
extraction (L). Extracts from noninoculated tomato using the FastPrep method
served as controls. Bars with
different letters are significantly different (P < 0.05, Student NewmanKeuls
method, n = 1415).

22

The reduction or removal of PCR inhibitors is an essential component in

the molecular detection of Cryptosporidium in faecal and environmental
samples. Currently, pathogen isolation by Immuno Magnetic Separation
(IMS) and culture enrichment prior to DNA extraction are standard procedures to eliminate or reduce PCR inhibitors. These methods, however,
become impractical for organisms that have no IMS procedures or that
cannot be cultured. The use of IMS is also expensive, and this limits the
use of samples mostly to single organism detection. Thus, the development of methods for direct extraction of PCR quality DNA is important for
the detection of pathogens in environmental samples.

Corneas are excised from frozen eyes of mice, and RNA is prepared using
the FastRNA Pro Green kit. Each cornea is placed in 0.8 ml RNApro
solution and homogenized with Lysing Matrix D in a FastPrep instrument
at setting 6.0 for 40 s. After cooling on ice, supernatants are transferred
and the lysing matrices rinsed with 0.2 ml RNApro solution. Combined
supernatants are chloroform-extracted, and RNA is precipitated from the
upper phase with an equal volume of isopropanol overnight at -20 C.
Pellets are rinsed with 70% ethanol, air-dried, and resuspended in 10 l
DEPC-treated H2O at 55-60 C for 10 min.
Reference: Berglund S.R. et al. (2007) J. Investigative Dermatology Vol 127; 349-353.

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MP Biomedicals

QA
&

Is the FastPrep-24 System suitable for lysis of spores?

Coloured scanning electron micrograph (SEM) of Bacillus anthracis spores

Has the FastPrep-24 System been tested for DNA

isolation from blue-green algae?

Blue-green algae also called cyanobacteria are successfully lysed by the

FastPrep-24 Instrument. 2 published studies from Crosbie N.D. et al.
(Appl. Environ. Microbiol. (2003), Vol 69: 5716-5721) and Steward G.F. et
al. (App. Environ. Microbiol. (2004), Vol 70: 1455-1465) report the lysis of
cyanobacteria cells with the FastPrep System for DNA extraction experiments.
Crosbie N.D. et al. extracted DNA from 10ml of Synechococcus and Cyanobium cultures using the FastDNA Kit and the FastPrep Instrument.
DNA was used for PCR amplification. Steward G.F. et al. added cyanobacteria resuspended in a phenol-chloroform-isoamyl alcohol solution to
Lysing Matrix B tubes (0.1mm silica beads). Samples were homogenized
2 times for 10 seconds at speed 6.0 m/s with the FastPrep Instrument.
Following phase separation and DNA precipitation with ethanol, DNA resuspended in TE buffer was used as template of nested-PCR assays.

Bacterial and fungal spores either in culture or in environmental samples

are successfully lysed with the FastPrep-24 system.
Bacillus Subtilis spores in suspension in Lysing Matrix B tubes are processed 3 times for 40 seconds at speed 6.0 m/s with 1 minute cooling on
ice between each run. 98% spore lysis was confirmed by microscopy.
Hudson K.D. et al. (J. Bacteriol. (2005), Vol 183: 4317-4322) isolated proteins from Bacillus Subtilis spores for Western Blotting and Keijser B.J.F. et
al. (J. Bacteriol (2007), Vol 189: 3624-3634) purified RNA from cultures of
Bacillus Subtilis spores with the FastRNA Pro Blue Kit and used total RNA
for reverse transcription, labelling and hybridization on micro-array slides.
Anthrax is one of the most dangerous zoonotic infectious disease and has
been the first candidate for biological weaponry for over 80 years.
It is very difficult to detect anthrax DNA from soil because of the presence
of humic acid and many other nonsporulated and sporulated bacteria.
DNA was extracted from 1 g of soil artificially contaminated with spores of
Bacillus anthracis using a FastDNA SPIN Kit for Soil. Results of nested
and real-time PCR experiments indicates that one cell of B. anthracis in 1
g of soil is detected by this rapid and highly sensitive method. (Cheun H.I.
et al. J.Appl. Microbiol. (2005) Vol 95: 728-733).
2 published studies from Roesti D.et al. (Appl. Environ. Microbiol (2005),
Vol 71: 6673-6679) and Mincer T.J. et al. (Appl. Environ. Microbiol (2005),
Vol 71: 7019-7028) describe the extraction of DNA from bacterial and fungal spores included in soil cores and marine sediments with the FastDNA
SPIN Kit for Soil. Purified DNA was used for seminested PCR, environmental library construction and DGGE analysis.

Is the FastPrep-24 System designed for the homogenization of hair samples for forensic toxicology analysis?

Synechococcus cyanobacteria

How to use the FastPrep-24 System for allergan

extractions from air-filter ?
A rapid and thorough extraction of endotoxin from PM2.5 air filters has
been developed at UCI by employing a high speed shaker (FastPrep-24,
MP Biomedicals, Solon, OH). Briefly, quartz filters were placed into endotoxin-free extraction vials containing pyrogen-free water and processed
by the FastPrep instrument for 30 seconds at 6.5 m/s. Following shaking, the samples were put onto a tube rotator (Dynal Biotech) for 1 hour.
An aliquot was assayed using a Limulus Amoebocyte Lysate (LAL) kinetic
chromogenic assay (Pyrochrome Associates of Cape Cod, Falmouth, MA).
In addition, a negative control filter (blank) was extracted and analyzed. A
set of sixteen archived personal PM2.5 air filters were evaluated for the
presence of endotoxin. We found detectable endotoxin concentrations in
14 filter extracts with a range of 0.03 - 5.5 EU/mL and a mean concentration of 0.73 EU/mL. The blank filter showed no detectable concentrations
of endotoxin.

The FastPrep-24 instrument combined with the TeenPrep adapter has

been successfully used for the lysis of hair samples prior to drug analysis. Uncut hair samples are added to 15ml Lysing Matrix tubes containing 8
large 1/4 inch ceramic spheres and 4 steel beads, samples are homogenized
2 times for 50 seconds at speed 6.0 m/s. After the homogenization step,
methanol is added to the powder-like homogenate for drug extraction.

Recommendations for the homogenization of small

amounts of animal and plant tissues (less than 10mg)
The homogenization of sample amounts lower than 10mg is performed with
Lysing Matrix D tubes after removal of half of the ceramic beads from the
tubes. Samples are disrupted at a speed setting of 6.0 m/s for 30 second

Quartz filter folded into the 15 ml tube

in a TeenPrep adapter

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Quartz filter lysate after FastPrep lysing with

no additional lysing matrix, for 30 seconds at
6.5 m/s speed settings

23

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