HY-RiSE® Colour Hygiene Test Strip

An analyser-free rapid test for qualitative testing of cleanliness of surfaces

What is the purpose of test? What is the test good for?

HY-RiSE® indicates surface cleanliness by measuring product • Monitoring of surface cleanliness of
residues, e.g. from food and beverages, left after inadequate – food and beverage production and storage areas as well
cleaning. as transport equipments
Typical applications are assessments of food and hand contact – cooking areas, food and beverage preparation and
surfaces, e.g. worktops, slicers, cutting boards, fridge handles, storage areas, especially after deep cleaning
microwaves, ovens, etc.
– toilets, bath rooms, rest rooms
Measurements made with HY-RiSE® can provide early warning
• Supervising and training/instructing of food and beverage
of possible contamination on specific surface areas allowing
producers resp.
immediate corrective action, e.g. removing food soils.

What does the test measure? Which are the benefits?

• Analyser-free hygiene test:
• Nicotinamide Adenine Dinucleotides (NAD, NADH)
– no investment cost
• Nicotinamide Adenine Dinucleotide phosphates (NADP,
NADPH) – portable in your pocket
These are a group of compounds found in all living cells like – applicable at any time and place
animal and vegetable matter, including food and food debris, in – offers low-end alternative to Merck HY-LiTE®
addition to bacteria, fungi and other microorganisms. • Test takes 3 drops of reagents on strip:
How does the test work? – easy to perform
– easy to dispose
• Clear test result available within 5 min:
– dirty surfaces can be re-cleaned immediately
– simple Yes/No answer
• Test is much more sensitive than visual inspection of
cleanliness:
– can be included into HACCP procedures
– provides confidence that HACCP regulations are met
– improves level of hygiene risk management
– can be used as a tool to identify risk areas for
microbiological testing
• Test is more sensitive than protein test
• Test is an essential part of the service package of contract
cleaners:
If NAD(P) and/or NAD(P)H is present in the sample, Gluc-DH®
converts ß-D-Glucose into D-Gluconolactone, then Diaphorase – enhances cleaning service package
converts a Tetrazolium Salt into a coloured Formazan Salt. – assures validated cleaning of HCPs
Any colour development on the test strip within minutes indi-
cates a positive result:
surface not clean.

Who can use the kit?

• small to medium size food and beverage producers
• cleaning companies, contract cleaners
• caterers, restaurants, canteens, hotels
• super markets, retailers, butchers, bakehouses
• hygiene inspectors, HACCP trainers

Merck Microbiology Manual 12th Edition 581

 HY-RiSE® Colour Hygiene Test Strip
An analyser-free rapid test for qualitative testing of cleanliness of surfaces.
HY-RiSE® Colour Hygiene Test Strip

Kit components 3. Sampling for testing surfaces:

Package for 50 tests consists of In case of smooth surfaces, place the entire test strip pad onto
• 50 foil wrapped test strips the test surface and draw back approximately 30 cm in
sample area.
• bottle A with wetting solution
On rough surfaces, collect sample by pressing the test strip
• bottle B with substrate solution pad onto a minimum of ten different spots on the surface. For
• bottle C with enzyme solution hand testing, sample each fingertip and five spots on the
• colour chart and insert sheets palm of the hand.

Preparation and storage

Test strips and solutions are ready-for-use.
Strips and solutions stable up to the expiry date stated on the
package when stored at +2 to +8 °C. Protect from light.
After first opening of each bottle and while in use, stable for 12
weeks when stored at +20 to +25 °C, if immediately re-sealed
with the same screw cap after each use.
Alternative: After first opening of each bottle and while in use,
Smooth surface Rough surfaces
stable for 6 months when stored at +2 to +8 °C, if immediately
re-sealed with the same screw cap and refrigerated after each
use.

Procedure
1. Tear open the foil package at the coloured line, remove the
test strip from the foil.

Hands

Sampling for testing Clean in Place (C.I.P) rinse waters:

Do not use Reagent A. Dip the test strip pad into the rinse
water so that half of the pad is wet. Alternatively, use a
pipette and transfer 30 µl sample onto the pad.

2. Add one drop of Reagent A to the pad on the strip.

Note: After sampling, the test strips can be left for up to 2 hours
at room temperature (up to 25 °C) before further processing, if
they are returned to the packaging.
4. Add one drop of Reagent B (substrate solution, yellow cap)
Note: If the test surface is wet, do not use Reagent A. to the test strip pad.

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 HY-RiSE® Colour Hygiene Test Strip

5. Add one drop of Reagent C (enzyme solution, blue cap) to the Literature
test strip pad. Schalch B, Trautsch M, Watkins I, Kau P, Stolle A, 2003: Einsatz eines
Schnelltests zur Untersuchung der Oberflächenreinheit (use of a new rapid
test for checking surface cleanliness - Archiv für Lebensmittelhygiene 54,
58-59.
Goll M, Kratzheller B, Bülte M, 2003: Kontrolle von Rückständen auf Ober-
flächen. Evaluierung des HY-RiSE® Colour Hygiene Test Strips zur Überprü-
fung der Sauberkeit (Evaluation of HY-RiSE® Colour Hygiene Test Strip for
checking the cleanliness of surfaces) - Fleischwirtschaft 9, 152 - 154.
Gierse S, Babel W: Zum Einsatz von Hy-RiSE® im Rahmen der
betriebseigenen Kontrolle nach §§3 und 4 LMHV - Bb Bundesverband der
beamteten Tierärzte, Kongress am 22./23. April 2002 , Bad Staffelstein.

6. Return test strip to foil packaging so that the pad is covered. Ordering Information
Leave for 4-5 minutes in the dark at +15 to +30 °C after
addition of Reagent C. Product Merck Cat. No. Description
HY-RiSE® 1.31200.0001 Colour
Hygiene Test
Strip
for assessing
the general
cleanliness of
surfaces.
Kit for 50
tests.

7. Yellow colour of the pad indicates a Clean/PASS condition.

There are no or undetectable levels of residue on the surface.
Pink/purple to bluish violet colouring of the pad indicates a
Dirty/FAIL condition. There are detectable levels of residue
on the surface. The surface should be re-cleaned. PASS/FAIL
conditions are marked clean √ and dirty ΧΧ .

clean/pass dirty/fail, re-cleaning is recommended

8. Document test results on the table to be found in the test kit.

Merck Microbiology Manual 12th Edition 583