Daily Maintenance Training
Bayu Setiyo Raharjo
PT. Genecraft Labs
Proprietary & Confidential The world leader in serving science
Daily Maintenance Training in
ICPMS – iCAP RQ
Contamination Sources in ICPMS
The ICP ion source for ICP-MS
• ICPs have the following advantages for both ICP-OES and ICP-MS:
• High efficiency ion source:
• High sensitivity
• Wide elemental coverage
• High matrix tolerance
• Atmospheric ion source – high flexibility for coupling of sampling devices
• This can be (roughly) summarized as:
What goes IN, comes OUT !
• And this is both an advantage and disadvantage of ICP-MS !
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What Goes IN...
Ar, Air
(O, N, C)
H2O,
Ca, Na,
K, Mg,
Cl, etc
Reactants
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…Comes OUT !
Ar, Air
(O, N, C)
ArAr, ArO, ArN,
ArC, ArH, ArCa,
ArNa, ArK, ArMg,
ArCl, ClO, NO, H2O,
CO, CaO, NaO, Ca, Na,
etc K, Mg,
Cl, etc
Products Reaction
Reactants
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Typical spectra interference of ICP-MS
Element m/z Interfences
K 39 ArH
Ca 40 Ar
V 51 ClO, ArC
Cr 52 ArC, ClOH
Mn 55 ClO
Fe 56 ArO
Co 59 ArNa
Cu 63 ArNa
Zn 66 ArMg
As 75 ArCl
Se 80 ArAr
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How to Deal with Polyatomic ions interference?
• Collision Reaction Cell Technology (CRCT)
• A multipole enclosed in a cylinder
• Controlled flow of gas into the cell
• Interaction of ions with the gas
• If reactive gas used, reactions occur
• If non-reactive gas is introduced, a loss of kinetic energy occurs
M+ only out
M+ and
XnYn’+
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CCT: Energy Discrimination
* ArAr+ + He ArAr+ + * He
*
*
In Thermo this Called KED
Kinetic Energy Discrimination
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CCT: Reactive Chemistry
ArAr+ + H2 ArH+ + ArH
Other gases for reactive chemistry: NH3, O2, Xe etc
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Interference Removal Collision Cell
Example : 56Fe Flatapole
He KED Mode
w/o Cut-Off Flatapole
Ar
40Ar16O
He He
He
O
56Fe He
O He
He
Ar
40Ar16O KED
Flatapole
Flatapole
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Interference Removal Collision Cell
Example : 56Fe Flatapole
He KED Mode
With Cut-Off Flatapole
Ar m/z = <39
He He
He
O
56Fe He
O He
He
Ar
40Ar16O KED
Flatapole
Flatapole
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Daily Maintenance Training in
ICPMS – iCAP RQ
Handling daily maintenances
Sample Introduction
Sample
Gas lines
Torch
holder
Peltier
cooled
Spray
chamber
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Sample Introduction
Peristaltic
Pump
Torch and
Injector
Nebulizer
Spray
Chamber
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Sample Introduction - Nebulizer
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Nebulizer Cleaning
• Cleaning using ELUO Nebulizer Cleaner
• Cleaning using Detergen Solution 25% in 4-6 hours
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Nebulizer Cleaning
• Cleaning using HNO3 10% overnight
• Cleaning using Aqua Regia 10% ≥ 4 hours
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Spray Chamber Cleaning
• Cleaning using Detergen Solution 25% in 4-6 hours
• Cleaning using HNO3 10% or Aqua Regia 10% overnight
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Torch
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Torch and Injector Cleaning
• Using HNO3 10% or Aqua Regia 10% overnight
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Sample and Skimmer Cones
• Sample Cones
• Skimmer Cones
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Sample and Skimmer Cones Cleaning
• Ultrasonic using Formic Acid 10% in Sonicator Bath
1. Take Sample cone, Skimmer and Insert cones in different beaker glass and fill with Formic Acid 10%
and ultrasonic for ±5 mins
2. Rinse Sample cone, skimmer and insert cones with DI water
3. Put sample cone, skimmer and insert cones in different beaker glass again and fill with DI water,
ultrasonic for ±5 mins
4. Repeat step 2 and step 3, and Dry Sample, Skimmer and insert cones in Fiberles tissue
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Extract Lens or Ion Lens
• Ultrasonic using HNO3 5% in sonicator bath
1. Reassamble Lens in ICPMS, Take each part in each beaker glass
2. Add HNO3 5% in each beaker glass, and ultrasonic for ±5 mins
3. Rinse with DI water, and add DI water in each beaker glass then ultrasonic for ±5 mins
4. Repeat step 3, 1 more time.
5. Rinse with DI water and dry each extract lens use Fiberles tissue
6. Assamble Ion lens when each part already dry and clean
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FAQs
FAQS
• Tuning always fail booth in STD mode or Gas mode (KED mode in
Thermo)
• Check Sample peristalic pump, apakah ada bubble ?
Jika Ya, maka coba cek sambungan antara sample peristaltic pump, apakah ada kotoran atau
debu yang menyumbat ?
Jika Ya bersihkan debu tersebut.
Apabila aliran sudah lancar dan tuning masih dibawah spesifikasi, matikan plasma atau cek
melalui camera plasma.
Cek apakah sample cones dalam keadaan kotor ? Jika Ya, maka bersihkan
• Intensity didn’t stable when tuning, stability higher than spesification but
insensity already meet requirement
• Check aliran pada sample peristaltic pump, jika aliran ada bubble lakukan seperti arahan
sebelumnya.
Jika lancar maka buka bagian pendingin spray chamber apakah aerosol terbentuk ?
Jika tidak, maka kemungkinan nebulizer tersumbat.
Lakukan Back Flush pada nebulizer menggunakan gas argon pada nebulizer
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