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A. Enzyme Concentration

absorbance and rate of reaction

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inshirahizham
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79 views3 pages

A. Enzyme Concentration

absorbance and rate of reaction

Uploaded by

inshirahizham
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
Available Formats
Download as DOCX, PDF, TXT or read online on Scribd

RESULT

a. Enzyme concentration

Cuvette OD OD OD Mean s.d


(replicate 1) (replicate 2) (replicate 3)

1 0.252 0.253 0.251 0.251

2 0.225 0.220 0.221 0.221

3 0.217 0.215 0.216 0.216

4 0.201 0.202 0.202 0.2017

Graph 1:

Enzyme concentration
0.3

0.25

0.2
Absorbance

0.15

0.1

0.05

0
0 0.5 1 1.5 2 2.5 3 3.5 4 4.5
cuvette
b. Substrate concentration

Cuvette OD OD OD Mean s.d


(replicate 1) (replicate 2) (replicate 3)

1 0.089 0.089 0.090 0.0893

2 0.139 0.139 0.139 0.1387

3 0.1724 0.174 0.174 0.174

4 0.392 0.392 0.390 0.391

5 0.332 0.330 0.327 0.330

6 0.370 0.371 0.371 0.371

7 0.411 0.409 0.411 0.410

Graph 2:

Substrate concentration
0.45
0.4
0.35
0.3
Absorbance

0.25
0.2
0.15
0.1
0.05
0
0 1 2 3 4 5 6 7 8
Cuvette
c. pH

Cuvette OD OD OD Mean s.d


(replicate 1) (replicate 2) (replicate 3)

1 0.236 0.237 0.237 0.2365

2 0.094 0.094 0.100 0.096

3 0.200 0.198 0.202 0.200

4 0.206 0.215 0.216 0.212

5 0.199 0.196 0.196 0.197

6 0.226 0.226 0.226 0.226

7 0.411 0.409 0.411 0.410

Graph 3:

pH
0.45
0.4
0.35
0.3
Absorbance

0.25
0.2
0.15
0.1
0.05
0
0 1 2 3 4 5 6 7 8
Cuvette

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