Scribd
Upload
134 views3 pages

Microbiology Lab Exercises Overview

This document outlines exercises for a microbiology lab, including identifying parts of a microscope, describing prokaryotic and eukaryotic cells, explaining Gram staining and acid-fast staining techniques and reagents, demonstrating methods for capsule staining, identifying tools for sterilization and culture, describing bacterial structure, and performing antimicrobial susceptibility testing.

Uploaded by

Nezel Anne Doromal
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
Available Formats
Download as DOCX, PDF, TXT or read online on Scribd
134 views3 pages

Microbiology Lab Exercises Overview

This document outlines exercises for a microbiology lab, including identifying parts of a microscope, describing prokaryotic and eukaryotic cells, explaining Gram staining and acid-fast staining techniques and reagents, demonstrating methods for capsule staining, identifying tools for sterilization and culture, describing bacterial structure, and performing antimicrobial susceptibility testing.

Uploaded by

Nezel Anne Doromal
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
Available Formats
Download as DOCX, PDF, TXT or read online on Scribd

Nezel Anne B.

Doromal BSN 1-A


Microbiology and Parasitology Lab
EXERCISE 1. Parts of the Microscope

EXERCISE 2. The Cell

Prokaryotic Cell

Eukaryotic Cell
Nezel Anne B. Doromal BSN 1-A
Microbiology and Parasitology Lab
EXERCISE 3. Gram-straining

Reagent Function Gram-positive Gram-negative


Crystal Violet Primary stain Purple or Blue Purple or Blue
Gram’s Iodine Mordant Purple or Blue Purple or Blue
Acetone or 95% Decolorizer Purple or Blue Colorless
alcohol
Safranin Counterstain or Purple or Blue Red or Pink
secondary stain

EXERCISE 4. Acid-fast staining


ZIEHL-NEELSEN STAIN (Hot method)

Reagent Function Acid-fast Non-acid-fast


Carbol-fuchsin Primary stain Red or Pink Red or Pink
Acid alcohol Decolorizer Red Colorless
Methylene blue Counterstain or Red organism / Blue Blue organism / Blue
secondary stain background background

KINYOUN STAIN (Cold method)

Reagent Function Acid-fast Non-acid-fast


Carbol-fuchsin Primary stain Red or Pink Red or Pink
Acid alcohol Decolorizer Red Colorless
Malachite green Counterstain or Red organism / Green Green organism /
secondary stain Background Green background

*Capsule staining (halo is the capsule)


– (using the india ink method) purple cells surrounded by a clear halo on a dark background
– (anthony’s stain method) purple cells surrounded by a clear or faint blue halo on transparent background

EXERCISE 5. Sterilization and Disinfection

PETRI DISH
ALCOHOL
LAMP
AGAR or AGAR PLATE

COTTON SWAB
TEST TUBES INOCULATING LOOP
APPLICATOR
Nezel Anne B. Doromal BSN 1-A
Microbiology and Parasitology Lab
EXERCISE 6. Bacterial Structure

EXERCISE 7. Antimicrobial Susceptibility Testing

You might also like