PROTEINS
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PROTEINS
• The Molecules which yields amino acids upon
hydrolysis are called proteins.
• Proteins are natural polymer of amino acids.
• The number of amino acids in a protein
molecule may range from two to several
thousands.
• Protein molecules contain Nitrogen, Carbon,
Hydrogen and Oxygen.
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PROTEINS
• Proteins are the basis for the major structural
components of animal and human tissue.
• They act as biological catalysts (Enzymes),
form structural parts of organisms, participate
in different cell reactions, act as molecules of
immunity and also provide fuel.
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CLASSIFICATION OF PROTEINS
(a) Simple Proteins
• Those which give one amino acid only upon
hydrolysis.
(b) Conjugated Proteins
• Those which give an amino acid and a non-protein
group upon hydrolysis.
(c) Derived Proteins
• Those which are derived from simple and conjugated
proteins.
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(a) SIMPLE PROTEINS - Albumins
Blood Milk Egg White Lentils
(Serumbumin) (Lactalbumin) (Ovolbumin) (Legumelin)
Kidney Beans Wheat
(Phaseolin) (Leucosin)
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(a) Simple Proteins - Albumins
• Globular protein is insoluble in water and
dilute salt solution.
• Precipitated by saturation with (NH4)2SO4
solution.
• Coagulated by heat.
• Found in plant and animal tissue.
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(a) Simple Proteins - Globulins
Blood (Serum Muscles (Myosin) Potato (Tuberin) Brazil Nuts
Globulins) (Excelsin)
Hemp (Edestin) Lentils (Legumin)
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(a) Simple Proteins - Globulins
• Globular Protein is sparingly soluble in
water and neutral solutions.
• Precipitated by dilute Ammonium
Sulphate.
• Coagulated by Heat.
• Distributed in both plant and animal
tissues.
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(a) Simple Proteins - Glutelins
Wheat (Glutenin) Rice (Oryzenin)
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(a) Simple Proteins - Glutelins
• Insoluble in water and dilute salt solutions.
• Soluble in dilute acids.
• Found in grains & cereals.
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(a) Simple Proteins - Histones
• Thymus Gland, Pancrease and
Nucleoproteins (Nucleohistones).
• Soluble in water, salt solutions & dilute
acids.
• Insoluble in Ammonium Hydroxide.
• Yeilds large amount of Lysine & arginine.
• Combined with nucleic acids within cells.
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(a) Simple Proteins - Histones
• Thymus Gland, Pancrease and
Nucleoproteins (Nucleohistones).
• Soluble in water, salt solutions & dilute
acids.
• Insoluble in Ammonium Hydroxide.
• Yeilds large amount of Lysine & arginine.
• Combined with nucleic acids within cells.
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(a) Simple Proteins - Scleroproteins
• Connective tissues and hard tissues.
• Fibrous protein is insoluble in all solvents.
• Resistant to digestion.
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(a) Conjugated Proteins - Nucleoproteins
Cytoplasm of Cells Nucleus of Chromosomes & Viruses &
(Ribonucleoprotein) Ribosomes Bacteriophage
(Deoxyribonucleoprotein)
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(a) Conjugated Proteins - Nucleoproteins
• It contains nucleic acids, nitrogen and
phosphorus.
• It is present in chromosomes and in all
living forms as a combination of protein
with either DNA or RNA.
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(b) Conjugated Proteins - Mucoprotein
• Saliva (Mucin) and Egg white
(Ovomucoid).
• Proteins combined with amino sugars,
sugar acids and sulfates.
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(b) Conjugated Proteins - Glycoproteins
• Bones (Osseomucoid), Tendons
(Tendomucoid) and Cartilage
(Chondromucoid).
• Containing more than 4% Hexosamine,
mucoproteins; if less than 4%, then
Glycoproteins.
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(b) Conjugated Proteins – Phosphoproteins
• Milk (Casein) and Egg yolk (Ovovitellin).
• Phosphoric acid joined in ester linkage to
protein.
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(c) Derived Proteins - Proteans
• Edestan ( from Elastin) and Myosin
(Myosin).
• It results from short action of acids or
enzymes
• Insoluble in water.
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(c) Derived Proteins - Proteases
• Intermediate product of protein digestion
• Soluble in water.
• Not coagulated by heat.
• Precipitated by saturated ammonium
sulphate
• Result from a partial digestion of protein
by pepsin or trypsin.
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(c) Derived Proteins -Peptones
• Intermediate product of protein digestion
• Same properties as proteases except that
they cannot be salted out.
• Smaller molecular weight than proteases.
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(c) Derived Proteins - Peptides
• Intermediate product of protein digestion
• Two or more amino acids joined by a
peptide linkage.
• Hydrolyzed to individual amino acids.
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STRUCTURE OF PROTEINS
• Depends upon the spatial arrangement of
polypeptide chains.
• Three arrangements are possible.
• Four structures:
i. Primary structure
ii. Secondary structure
iii. Tertiary Structure
iv. Quaternary Structure
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The Primary Structure Of Proteins
• The sequence of amino acids in a
polypeptide chain is called a primary
structure.
• Amino Acids are linked with one another
through peptide bonds.
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The Primary Structure Of Proteins
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The Secondary Structure Of Proteins
• Peptide chains may acquire spiral shape
or may be present in a zig zig manner.
• This coiling of peptide chains is called the
secondary structure of proteins.
• It is due to Hydrogen bonding.
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The Secondary Structure Of Proteins
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The Tertiary Structure Of Proteins
• Twisting or folding
of polypeptide
chains represents
tertiary structure of
proteins.
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The Quaternary Structure Of Proteins
• Quaternary means four.
• It is the arrangement of multiple folded
protein or coiling protein molecules in a
multi-subunit complex.
• A variety of bonding interactions including
Hydrogen bonding, salt bridges and
disulfide bonds holds the various chains
into a particular geometry.
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The Quaternary Structure Of Proteins
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Properties Of Proteins
• Found in all living organisms.
• Involved in processes such as digestion of
food, cell structure, catalysis, movement,
energy manipulation etc.
• Complex molecules.
• Polymers of amino acids.
• Long chains of amino acids are called
Polypeptides.
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Importance of Proteins
• Proteins play an important role in
formation of protoplasm.
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Importance of Proteins
• Nucleoproteins are
complex proteins and
act as the carrier of
heredity materials from
one generation to
another.
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Importance of Proteins
• Enzymes are the biological catalyst and
they are also proteins
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Importance of Proteins
• Hemoglobin is a protein. It act as oxygen
carrier.
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Importance of Proteins
• Tanning of hides is actually precipitation of
proteins by tannic acid.
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Importance of Proteins
• Gelatin is obtained by heating bones,
skins and tendons in water. It is used in
bakery goods.
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Importance of Proteins
• Casein is another protein used in
manufacture of buttons and buckles.
• Proteins obtained from soya bean are
used for manufacture of plastics.
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