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Merode Numerik

The document describes an experiment to determine the relationship between the concentration of substrate [S] and the rate of enzymatic reaction [E]. Data was collected for varying concentrations of [S] and the corresponding values of [E] were recorded. The data was fitted to a linear regression model to determine the kinetic parameters k2 (turnover number) and Km (Michaelis constant). The regression analysis found k2 = 22.54249 and Km = 1.69806.

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42 views6 pages

Merode Numerik

The document describes an experiment to determine the relationship between the concentration of substrate [S] and the rate of enzymatic reaction [E]. Data was collected for varying concentrations of [S] and the corresponding values of [E] were recorded. The data was fitted to a linear regression model to determine the kinetic parameters k2 (turnover number) and Km (Michaelis constant). The regression analysis found k2 = 22.54249 and Km = 1.69806.

Uploaded by

wahyu adi pramono
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
Available Formats
Download as XLSX, PDF, TXT or read online on Scribd

[S] g s/l [E] = 0,005 g/l X=1/[S] Y= [E]/r

1 0.055 1 0.055
2 0.099 0.5 0.0495
5 0.193 0.2 0.0386
0.06
f(x) = 0.038754244425728 x + 0.022
7.5 0.244 0.133333333 0.032533333 R² = 0.77414787822836
10 0.28 0.1 0.028
0.05
15 0.333 0.066666667 0.0222
20 0.365 0.05 0.01825
0.04
30 0.407 0.033333333 0.013566667

1 1 0.055 0.03
1 0.5 0.0495
1 0.2 0.0386 0.02
1 0.133333 b0 0.032533333 = y
Xb = 1 0.1 0.028 0.01
1 0.066667 0.0222
1 0.05 0.01825
0
1 0.033333 b1 0.013566667 0 0.2 0.4

[E0] = 0.

[Link]= Xtranspose.y

Xtranspose = 1 1 1 1 1
1 0.5 0.2 0.133333333 0.1

8 2.083333
2.083333333 1.325833
[E0] = 0.005 vs 1/[S]

f(x) = 0.038754244425728 x + 0.022113998847467


R² = 0.77414787822836

0 0.2 0.4 0.6 0.8 1 1.2

[E0] = 0.005 vs 1/[S] Linear ([E0] = 0.005 vs 1/[S])

1 1 1 1 1 1 1
0.066667 0.05 0.033333 1 0.5 1 0.5
1 0.2
1 0.133333 b0 =
1 0.1
1 0.066667
1 0.05
1 0.033333 b1

b0 = 0.25765
b1 0.097453

b0 = 0.159582 1.325833 -2.083333 0.25765


-2.083333 8 0.097453

b0 = 0.159582 0.138575
b1 = 0.242849

b0 = 0.022114
b1 = 0.038754
k2 = 45.22022
km = 1.752476
1 1 1 1 1 1 0.055
0.2 0.133333 0.1 0.066667 0.05 0.033333 0.0495
0.0386
0.032533
0.028
0.0222
0.01825
0.013567
[S] g s/l [E] = 0.01 g/l X=1/[S] Y= [E]/r
1 0.108 1 0.108
2 0.196 0.5 0.098
5 0.383 0.2 0.0766 [E
7.5 0.488 0.133333 0.065067 0.12
10 0.56 0.1 0.056 f(x) = 0.075327053504145 x + 0.044
R² = 0.765329495299062
15 0.665 0.066667 0.044333 0.1
20 0.733 0.05 0.03665
30 0.815 0.033333 0.027167 0.08

1 1 0.108 0.06
1 0.5 0.098
1 0.2 0.0766 0.04
1 0.133333 b0 0.065067 = y
Xb = 1 0.1 0.056 0.02
1 0.066667 0.044333
1 0.05 0.03665 0
0 0.2 0.4
1 0.033333 b1 0.027167
[E0] = 0.005 vs 1
Linear ([E0] = 0.0

[Link]= Xtranspose.y

Xtranspose = 1 1 1 1 1 1 1 1
1 0.5 0.2 0.133333 0.1 0.066667 0.05 0.033333

8 2.083333 b0 = 0.511817
2.083333 1.325833 b1 0.192289

b0 = 0.159582 1.325833
-2.083333

b0 = 0.159582 0.277981
b1 = 0.472029

b0 = 0.044361
b1 = 0.075327
k2 = 22.54249
km = 1.69806
[E0] = 0.01 vs 1/[S]

f(x) = 0.075327053504145 x + 0.044360663149962


R² = 0.765329495299062

0.2 0.4 0.6 0.8 1 1.2

[E0] = 0.005 vs 1/[S] Linear ([E0] = 0.005 vs 1/[S])


Linear ([E0] = 0.005 vs 1/[S])

1 1 1 1 1 1 1
1 0.5 1 0.5 0.2 0.133333 0.1
1 0.2
1 0.133333 b0 =
1 0.1
1 0.066667
1 0.05
1 0.033333 b1

-2.083333 0.511817
8 0.192289
1 1 1 0.108
0.066667 0.05 0.033333 0.098
0.0766
0.065067
0.056
0.044333
0.03665
0.027167

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