AGR3701 – EXPERIMENTATION AND AGRICULTURE BIOMETRY EXERCISE
AGR3701 EXPERIMENTATION AND AGRICULTURE
BIOMETRY
Exercise 6
Experimental Design RCBD
Name: INTAN AFIQAH BINTI AHMAD FADZIL
Matrics no: 202152
Lecturer’s name: Dr. Nazatul Shima Naharudin
1
AGR3701 – EXPERIMENTATION AND AGRICULTURE BIOMETRY EXERCISE
EXERCISE 6 – EXPERIMENTAL DESIGN – RCBD
Due: 31 December 2020
ANOVA of RCBD
An experiment to evaluate the effect of four types of fertilizer on yield of eggplant was conducted in a
randomized complete block design (RCBD) with 3 blocks. The blocking was due to slope in the field. The table
below shows the yield of fruits (kg/plot) from the experiment.
Fertilizer Rep 1 Rep 2 Rep 3
CM 31 31 30
CM+OF 39 34 39
NPK 45 37 39
NPK + BC 44 36 40
N source: OF = Organic fertilizer, CM = Chicken manure, PE=Plant enhancer,
NPK = NPK fertilizer, BC = Biochar
1. Draw the experimental layout of this experiment. Label the direction of variation, block and treatment.
T1 T3 T2 T4 Rep (Block 1)
T2 T3 T4 T1 Rep (Block 2)
T3 T4 T1 T2 Rep (Block 3)
2. State the null and alternative hypotheses for fertilizer treatment and block.
HO: There is no significant difference between types of fertilizer on yield of eggplant.
HA: There is significant difference between types of fertilizer on yield of eggplant
2
AGR3701 – EXPERIMENTATION AND AGRICULTURE BIOMETRY EXERCISE
3. Calculate:
i. the means for all blocks and fertilizer treatments
ii. grand mean
Grand mean treatment = 37.083
Grand mean block = 37.073
iii. the sum of square (SS) for total, treatment, block and error
3
AGR3701 – EXPERIMENTATION AND AGRICULTURE BIOMETRY EXERCISE
SSerror = 28.8333
4. Construct and complete the ANOVA table. Calculate mean squares and F values. Use F table or F.DIST.RT
(x, df1, df2) function in Excel to determine the critical values of F.
ANOVA
Source of
Variation SS df MS F P-value F crit
Treatment 180.9167 3 60.30556 12.54913 0.005381 4.757063
block 55.16667 2 27.58333 5.739884 0.040443 5.143253
Error 28.83333 6 4.805556
Total 264.9167 11
5. Interpret the results of the experiment based on ANOVA. Are there significant differences among the
treatments? And block?
For a treatments and block, there is a significant difference between types of fertilizer on yield of eggplant as
Fcalc > than Fcritical, so we reject Ho.
4
AGR3701 – EXPERIMENTATION AND AGRICULTURE BIOMETRY EXERCISE
6. Conduct a mean comparison using LSD to compare between treatment means at alpha 0.05.
7. Present your result in a table. Label the mean with letter grouping.
8. Run ANOVA and mean comparison using R software. Show your ANOVA table below.
Analysis of Variance Table
Response: yield
Df Sum Sq Mean Sq F value Pr(>F)
fertilizer 3 180.917 60.306 12.5491 0.005381
block 2 55.167 27.583 5.7399 0.040443
Residuals 6 28.833 4.806
fertilizer **
block *
Residuals
---
Signif. codes:
0 ‘***’ 0.001 ‘**’ 0.01 ‘*’ 0.05 ‘.’ 0.1 ‘ ’ 1
Mean Comparison
$statistics
MSerror Df Mean CV t.value LSD
4.805556 6 37.08333 5.911437 2.446912 4.379701
9. Summarize your result in a figure using R (or Excel).
$groups
yield groups
3 40.33333 a - There are a significant difference among type of fertilizer.
4 40.00000 a - NPK produced the highest mean yield and CM produced the lowest mean yield.
2 37.33333 a - When NPK+BC is added; the amount of yield was almost the same as NPK fertilizer
1 30.66667 b
