Experiment 9
ACIDS and BASES
(MIDTERM)
OBJECTIVE:
Identify acids and bases using a plant indicator.
THEORY:
Acids, bases, and salts constitute the largest groups of chemical compounds. Acids in
solution break up into hydrogen ions (protons) and negative ions called anions. The protons
combine with water forming the hydronium ions and these positive ions are called cations.
Acids and bases except for salts are differentiated by their color change with indicators. The
indicators commonly used are blue and red litmus paper or pH paper. Other indicators may
be prepared from natural sources.
An acid reacts with base such that the anion of the acid combines with cation of the
base forming salt. This reaction between acid and base forming water and salt is called
neutralization.
MATERIALS:
Dropper, cooking stove, chopping board, knife, medicine droppers, flower petals,
cheesecloth, small bottles with stoppers, vinegar, calamansi, liquid soza, alum (tawas),
baking soda, carbonated drink, saliva, urine, alum (tawas), soap, and liquid detergent.
PROCEDURE:
Acids and Bases
Test the color reactions of the indicator with a solution of the following substances:
liquid soza, vinegar, calamansi, liquid detergent, carbonated drink, alum (tawas), saliva, and
urine. Use the data sheet to record your observations.
PREPARATION OF INDICATOR
1. Chop the flower petals and put about 2 tablespoons each in separate bottles. Add 1
teaspoon of rubbing alcohol into the first bottle and label it as “Indicator 1”. To the
second bottle, add 1 teaspoon of water and label it as “Indicator 2”. Mix and let the
mixtures stand overnight. Filter the mixture 1 and 2 through cheesecloth and put the
filtrates in clean bottles. These filtrates will serve as your plant indicator.
2. Add 2 drops of plant indicator soaked in rubbing alcohol to about 10 drops or 1
tablespoon of each solution: liquid soza, vinegar, calamansi, liquid detergent,
carbonated drink, alum (tawas), saliva and urine. Note the color produced.
3. Add 2 drops of plant indicator soaked in plain water to about 10 drops or 1
tablespoon of each solution: liquid soza, vinegar, calamansi, liquid detergent,
carbonated drink, alum (tawas), saliva and urine. Note the color produced.
DATA SHEET
Acids and Bases
Substance tested Indicator Indicator 2
1 Color
Color
Vinegar
Calamansi
Liquid soza
Liquid detergent
Carbonated
drink
Alum (tawas)
Saliva
Urine
GUIDE QUESTIONS for the explanation of results:
1. What color is shown by acidic substances when tested with the indicator? Basic
substance
2. Can the plant extract serve as an indicator to distinguish acids and bases? Why?
CONCLUSION:
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