Unit 3 Biology 2017
Outcome 1: Cellular Processes
Questions
Reading time: 5 minutes Writing time: 55 minutes
Name : ………………………………………………………………………………………………………………..
Teacher (circle): Mr Trajanovski Ms Nguyen
Question Booklet
Instructions
Section Number of Questions Numberlogbook.
of Marks
You should have pens, pencils, a ruler and an eraser, completed
Log book
Ensure that you write your name and circle your teacher’s name clearly on13 this booklet.
Part 1: Enzymes prac 9 (1 -9) 25
Failure to do so will result in a 2 marks deduction.
Part 2: Respiration prac in in the space 10provided
( 10 – 19) 15 booklet. Answer
Answer all Short Answer questions in this question and answer
Part 3: General
all Multiple Choice Questions by11 (20 -31) 24 (total 77)
circling your response on the question.
No marks will be deducted for incorrect answers.
Submit this question book AND log book when time is finished.
Counts as 50/100 marks for Unit 3 SACs.
This outcome requires the prior conduct and collection of data from 2 experiments:
1. Enzyme action 2. Cellular respiration
You must have your log book with you and refer to it.
PART 1: Investigating Enzymes
1. Write 3 suitable hypotheses for this experiment. (3 marks)
Hypothesis 1: …………………………………………………………………………………………………………………..
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Hypothesis 2: ………………………………………………………………………………………………………………….
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Hypothesis 3:
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2. Identify two controlled variables in this experiment (2 Marks)
Controlled variable 1
Controlled variable 2
3. On the grid below draw a suitable graph showing your results. Present the data in the form of a bar graph.
Include all correct scientific graphing convention. (4 marks) - 1 mark will be deducted for each incorrect or
missing item.
4. What is the scientific name and purpose of tube A? (2 marks)
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5. In comparison to tube A, the other tubes showed different results. Explain what has caused this difference in
each of the tubes. (6 marks)
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6. State the dependent variable in this experiment. (1 mark)
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7. Catalase is an enzyme.
a. What type of organic compound is it?
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b. What basic building blocks does it consist of?
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c. Where in the cell is it synthesised?
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8. State one other factor that can affect the rate of enzyme action. (1 mark)
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9. Within all cells, there are many chemical reactions occurring which are controlled by enzymes.
a) What general biological term is used when referring to all the chemical reactions in a cell? (1 mark)
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b) To which level of structure do we refer to if an enzyme is composed of more than 1 polypeptide chain? (1 mark)
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c) Briefly explain why there are so many different enzymes present within cells. (1 mark)
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PART 2: Yeast respiration
10. Write a suitable aim for this experiment. (1 mark)
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11. On the grid below draw a suitable graph showing your individual results. This will be a column graph for the 3
glucose concentrations. ( 4 marks – 1 mark deducted for each incorrect or missing item.)
12. Name the gas that the yeast is producing. (1 mark)
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13. Assuming that there is little or no oxygen available in the tubes, give the scientific and common names of the
process that the yeast is performing to produce this gas. ( 2 marks)
Scientific name: …………………………………………………………………………………………………………
Common name: ………………………………………………………………………………………………………..
14. As the concentration of glucose was increased what happened to the rate of gas production? (1 mark)
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15. Explain the difference in gas production observed in the 3 tubes tested in part B. (1 mark)
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16. Name the independent variable in this experiment. (1 Mark)
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17. Explain why this experiment was conducted at temperature warmer than room temperature. (1 mark)
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18. Give one other factor which would affect the rate gas production which we could test in a similar way. ( 1 mark)
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19. Suggest two sources of error in this experiment and suggest improvements you could make to minimise them.
(2 marks)
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Part 3: General Questions
20. Plant materials containing cellulose and other polyscaccharides are reacted with acid to break them down to
produce glucose. This glucose is then used by yeast cells for fermentation.
a. Why is fermentation important for yeast cells? (1 mark)
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b. What are the products of fermentation in yeast cells? (1mark)
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A by-product of the acid treatment of plant materials is a group of chemical compounds called furans. It has been
observed that as the concentration of furans increases, the rate of fermentation decreases. The enzyme alcohol
dehydrogenase is required for the process of fermentation.
c. Design an experiment to test the hypothesis that one of the furans, called furfural, is an inhibitor of the enzyme
alcohol dehydrogenase. Assume that the experiment will be repeated many times and environmental factors
will be kept constant. (4 marks)
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d. Scientists have proposed that furfural is a competitive inhibitor of the enzyme
alcohol dehydrogenase. Explain how furfural could act as a competitive inhibitor of the enzyme alcohol
dehydrogenase. (2 marks)
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21. The apparatus shown below was used in a series of experiments to study aerobic cellular respiration.
In 3 different experiments, the reaction tube initially contained the following:
1. Suspension of mitochondria
2. Cystosol of cells from which the mitochondria had been removed
3. Suspension of mitochondria and cytosol (ie mitochondria floating around in cysosol)
The temperatures and pH of the mixtures within the reaction tubes were carefully controlled so as not to damage the
mitochondrial structure or any of the enzymes.
In each experiment, a solution containing glucose was first added to the mixture in the reaction tube and the oxygen
concentration was measured for 3 minutes. Then, a pyruvate solution was added and the oxygen concentration was
measured again for 3 minutes.
Using your knowledge and understanding of aerobic respiration and mitochondria, complete the tables below with your
prediction of the change in oxygen concentration of the mixture in the reaction tube after the addition of each
substance and give a reason for your prediction. ( 6 marks)
Experiment 1 – Suspension of mitochondria
Substance added Change in oxygen concentration Reason
(increase/decrease/no change)
glucose
pyruvate
Experiment 2 – Cytosol of cells from which the mitochondria had been removed
Substance added Change in oxygen concentration Reason
(increase/decrease/no change)
glucose
pyruvate
Experiment 3 – Suspension of mitochondria and cytosol of cells
Substance added Change in oxygen concentration Reason
(increase/decrease/no change)
glucose
pyruvate
Multi Choice: Circle you answer.
22. ATP is important in living cells as it
A. Is required for osmosis
B. Provides a supply of usable energy for the cell
C. Provides one of the building blocks for lipid synthesis
D. Is an important structural component of the plasma membrane
23. Plants grown in light were supplied with water containing radioactive oxygen atoms. After 4 hours, an analysis of
the chemicals in and around the plants was undertaken. Which of the following would contain the radioactive
oxygen atoms after 4 hours?
A. Protein
B. Glucose
C. Oxygen gas
D. Carbon dioxide gas
24. Which of the following statements about photosynthesis on chloroplasts is correct?
A. The grana are the site of the light independent stage
B. Chlorophyll found in the stroma traps light for use during the light-dependent stage
C. The light-dependent stage produces ATP for use during the light independent stage
D. The light-independent stage captures carbon dioxide for use during the light dependent stage to produce
glucose
25. An experiment was conducted to investigate enzyme activity. A small quantity of amylase solution was added to
a solution of starch dissolved in water at 35 °C. It was observed that maltose was produced. Which one of the
following is the substrate in this reaction?
A. water
B. starch
C. maltose
D. amylase
Use the following information to answer Questions 26 and 27. Rotenone is a chemical compound that is used as an
insecticide and a piscicide (a substance that kills fish). The rotenone molecule disrupts the electron transport chain in
animal cells by interfering with one of the essential reactions within the electron transport chain.
26. Which one of the following statements best explains the effect of rotenone in causing death in insects and fish?
A. The rate of glycolysis would increase.
B. ATP would accumulate in the mitochondria.
C. Aerobic respiration in the mitochondria would be disrupted.
D. The cell membrane would no longer be permeable to oxygen.
27. In the past, people sometimes put extracts containing rotenone into a river to poison the fish, allowing the fish
to be more easily caught. When rotenone-poisoned fish are eaten by people, no poisonous effect is observed.
Which one of the following statements best explains this observation?
A. Rotenone is not absorbed through the cell membranes of people who have eaten poisoned fish.
B. Rotenone is not absorbed by fish tissue and remains dissolved in water.
C. Human cell metabolism does not involve the electron transport chain.
D. Rotenone only affects organisms that respire anaerobically.
28. During photosynthesis in chloroplasts, energy is used to split water, forming oxygen and hydrogen ions. The
splitting of water occurs
A. in the stroma during the light-independent reaction.
B. in the grana during the light-dependent reaction.
C. on the membrane of the thylakoids during the light-independent reaction.
D. on the surface of the outer chloroplast membrane during the light-dependent reaction.
29. An increase in the atmospheric CO2 level increases the rate of photosynthesis. The rate of photosynthesis
increases because
A. the rate of the light-independent reactions on the thylakoid membranes of the chloroplasts increases.
B. water loss from the leaf decreases, resulting in the availability of water for photosynthesis increasing.
C. the increased CO2 level lowers the pH inside the chloroplasts and increases the rate of enzyme-catalysed
reactions.
D. the rate of the light-independent reactions in the stroma increases with the increase in CO2 level.
30. If there is insufficient glucose for cellular respiration, fatty acids can be changed to acetyl CoA. Each fatty acid X
molecule produces eight molecules of acetyl CoA. The diagram below summarises the pathways for the
breakdown of fatty acid X and glucose. The number of molecules produced in each step is not shown.
Referring to the information above and your knowledge of cellular respiration, which one of the following
conclusions can be made?
A. Most of the ATP is made in the Krebs Cycle.
B. Pyruvic acid is converted to acetyl CoA under anaerobic conditions.
C. No ATP can be formed from the breakdown of glucose under anaerobic conditions.
D. One fatty acid X molecule produces more ATP in aerobic conditions than one glucose molecule does.
31. The following graphs show the way four enzymes, W, X, Y and Z, change their activity in different pH and
temperature situations.
Which one of the following statements about the activity of the four enzymes is true?
A. At pH 7, enzyme Y is denatured at temperatures below 20 °C.
B. Enzyme Z could be an intracellular human enzyme.
C. At pH 3 and a temperature of 37 °C, the active site of enzyme W binds well with its substrate.
D. At pH 3 and a temperature of 37 °C, enzyme X functions at its optimum.
