19th Annual WORLD CONGRESS INSULIN RESISTANCE
DIABETES & CARDIOVASCULAR DISEASE
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TAS2R38 taste receptor gene and metabolically unhealthy obesity REPLACE THIS BOX WITH
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Aleksandr Abaturov MD, DSc , Professor ; Anna Nikulina MD, PhD; Dmitriy Nikulin
Dnipro State Medical University, Ukraine
Background: Objective:
The spread of obesity has become pandemic, and it is expected that during to study the role of SNV gene TAS2R38 in the formation of MUO in children
2021 the number of obese children and adolescents (5 to 19 years) will be 6-18 years old.
158 million [World Obesity Federation, 2020]. There is an alarming trend
towards an increase in the incidence of metabolically unhealthy obesity MUO
(MUO), especially among children [Williamson K. et al., 2020].
Genetically determined violation of taste preferences leads to an inversion
of the perception of tastes and overeating, distorting the homeostatic
feedback of the peripheral energy status with hedonic centers, causing
obesity.
In our work, the importance of genetic variants of the taste 2 receptor
member 38 (TAS2R38) is presented as the most significant predictor of
metabolically unhealthy obesity among genes responsible for taste formation,
according to GWAS data. Fig. 1. Genetic variants of SNV TAS2R38 in the MUO phenotype.
Materials and methods Results Conclusion
90 obese children aged 6-18 years were examined. The main group (n = 52) The level of the average value of preference for bitter food in the main Decreased taste preferences for bitter foods increase the risk of developing
was represented by children according to the criteria of the consensus of the group was 2.75 ± 0.15 points, while in the control group 3.24 ± 0.14 points MUO in children.
International Diabetes Federation (IDF) with MUO. The control group (n = 38) and the Student's test in the compared populations was 2.39, with p <0.02 . The C/G genotype rs713598 has the greatest association among the SNV of
was formed by patients with MHO. To identify the prevailing modalities of There were no statistically significant differences in preferences for sour, salty the TAS2R38 gene detected by us with the formation of metabolically
taste preferences in the 5 most important categories (sweet, sour, fatty / and fatty / umami flavors in the comparison groups, p> 0.05. Analysis of food unhealthy obesity.
umami, salty and bitter), a survey was conducted using an adapted version of diaries in children showed a positive correlation between daily non-
the approved and used in the study IDEFICS (Identification and prevention of consumption of fresh vegetables and the formation of MUO (r = -0.32) with a
Dietary and lifestyle-induced health EFfects In Children and infantS Study) of prognostic factor of 2.7; p <0.05.
the Food and Beverage Preference Questionnaire (FBPQ) and an analysis of SNVs of the TAS2R38 gene (missense mutation) were diagnosed by
food diaries. To determine the SNV of the TAS2R38 gene in the formation of complete genomic sequencing (Table 1). The probability of detecting a
metabolically unhealthy obesity, we used the method of complete genomic gerozygotic variant of the C/G genotype rs713598 of the TAS2R38 gene in the
sequencing on the Illumina platform in a certified laboratory CeGat (Tübingen, main group was 1.75 times higher than in the control group of obese children,
Germany), followed by bioinformation analysis. p <0.05 (Table 2, Fig. 1).
For statistical processing of the obtained results the variational analysis
with definition of the criterion of reliability of Student, the relation of
chances, the correlation analysis of Spearman, calculation of the prognostic Table 2. SNV of the TAS2R38 gene in the MUO phenotype
factor is used. Funding
Genetic variant Probability of detection
The work is a fragment of the research work "Prediction of the
SNV TAS2R38 in MUO-phenotype
development of childhood diseases associated with civilization" (No.
Table 1. SNVs of the TAS2R38 gene (missense mutation) and CADD 10246939 T/C, HOM OR 1.167; 95%DI 0.098-14.06 0120U101324) of the Dnipro State Medical University. The study was carried
out in accordance with the program 2301020 "Scientific and scientific and
10246939 T/C, HET OR 1.193; 95%DI 0.33-4.28 technical activities in the field of health care", financed by the Ministry of
1726866 G/A, HOM OR 0.79; 95%DI 0.21-2.94 Health of Ukraine from the state budget. Informed Consent was obtained
from all participants included in the study.
1726866 G/A, HET OR 1.33; 95%DI 0.37-4.76 Authors’ contributions
AA was responsible for the idea and study design, looked over the articles, extracted the data, and
713598 C/G, HOM OR 1.167; 95%DI 0.098-14.06 interpreted bioinformatics analysis data. DN provided the collection of biological material using dried
blood spot shipping kit, AN analyzed the data and interpreted it. Both authors reviewed the paper and
713598 C/G, HET OR 1.75; 95%DI 1.1-6.35 approved the final manuscript.
Conflict of Interest: The authors declare that they have no conflict of interest.
Contact References
Aleksandr Abaturov, Dnipro State Medical University, Street 9, V. Vernadskogo, 49044, Dnipro, Ukraine, Honored Worker of Science and Technology
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*Corresponding author: Dmitriy Nikulin, Dnipro State Medical University, Street 9, V. Vernadskogo, 49044, Dnipro, Ukraine, 3rd year student of the estimation based on population genetics. Eur J Endocrinol. 2020 Jan;182(1):47-56. doi: 10.1530/EJE-19-0678.
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