228-92355F

Oct. 2022

UV-VIS Detector
For Shimadzu Ultra High Performance Liquid Chromatograph

SPD-40
SPD-40V

Instruction Manual
Read this manual thoroughly before you use the product.
Keep this manual for future reference.
This page is intentionally left blank.
Introduction
Read this Instruction Manual thoroughly before using
the product.
Thank you for purchasing this product.
This instruction manual describes the basic operation, and accessories and options for this
product. Read this manual thoroughly before using the product and operate the product
in accordance with the instructions in this manual.
The following instruction manuals are included with the product in booklet form or in
PDF format. The PDF documentation is on the instruction manual CD-ROM (Part No.
228-92350-41).

Document Name Document No. Description

Instruction Manual (PDF) 228-92355 This instruction manual.
This manual provides details on how to use
the system:
System Guide (PDF) 228-92352 system performance optimization, analysis
procedure, troubleshooting, validation,
installation, etc.
Safety Guideline Describes the precaution instructions to
228-92326
(Booklet/PDF) ensure safe operation.

Read "Safety Guideline" thoroughly before using the product.

"Safety Guideline" describes the information about the warranty, after-sales service,
safety instructions and precautions to ensure safe operation of the instrument. Keep this
manual for future reference.

Important
• If the user or usage location changes, ensure that this Instruction Manual is always
kept together with the product.
• If this manual or a product warning label is lost or damaged, immediately contact
your Shimadzu representative to request a replacement.
• To ensure safe operation, read the accompanying booklet "Safety Guideline"
before using the product.
• To ensure safe operation, contact your Shimadzu representative if product
installation, adjustment, re-installation (after the product is moved), or repair is
required.

ã 2019-2022 Shimadzu Corporation. All rights reserved.

Original version is approved in English.

SPD-40 SPD-40V i
 Introduction

Notice
• Information in this manual is subject to change without notice and does not represent
a commitment on the part of the vendor.
• Any errors or omissions which may have occurred in this manual despite the utmost
care taken in its production will be corrected as soon as possible, although not
necessarily immediately after detection.
• All rights are reserved, including those to reproduce this manual or parts thereof in
any form without permission in writing from Shimadzu Corporation.
• Nexera is a trademark of Shimadzu Corporation.
Windows is a registered trademark of Microsoft Corporation in the United States and/or
other countries.
Third-party trademarks and trade names may be used in this publication to refer to
either the entities or their products/services, whether or not they are used with
trademark symbol "TM" or "®".

Indications Used in This Manual

Precaution symbols are indicated using the following conventions:

Indication Meaning

Indicates a potentially hazardous situation which, if not

avoided, could result in serious injury or possibly death.

Indicates a potentially hazardous situation which, if not

avoided, may result in minor to moderate injury or equipment
damage.
Emphasizes additional information that is provided to ensure
the proper use of this product.

The following symbols are used in this manual:

Indication Meaning

Indicates an action that must not be performed.

Indicates an action that must be performed.

Indicates information provided to improve product performance.

Indicates the location of related reference information.

ii SPD-40 SPD-40V
 Electromagnetic Compatibility

Electromagnetic Compatibility
Descriptions in this section apply only to the following models:
• 228-65312-58 SPD-40
• 228-65313-58 SPD-40V
This product complies with European standard EN61326, class B for electromagnetic
interference (Emissions) and industrial electromagnetic environment (Immunity).

n EN55011 Emissions (Electromagnetic Interference)

This is a class B product. When this product causes an electromagnetic disturbance to

devices being used near this product, create an appropriate distance between those
devices and this product in order to eliminate the disturbance.

SPD-40 SPD-40V iii

Contents
Introduction Indications Used in This Manual ......................... ii
Electromagnetic Compatibility ............................ iii

1 Overview 1.1 Features ............................................................. 1

1.2 Component Parts ................................................. 2
1.3 Optional Parts ..................................................... 3
1.3.1 Optional Flow Cell .............................................. 3

Parts Identification 8
2 and Function
2.1 Front ..................................................................

2.2 Behind Front Cover, Top, and Left Side ................ 9

2.3 Back ................................................................. 11
2.4 Right Side and Bottom ...................................... 12
2.5 Names and Functions of Display and Operation
Area ................................................................. 13
2.5.1 Display Area ................................................... 15

2.5.2 Operation Area ................................................ 16

3 Operation 3.1 Precautions ....................................................... 18

3.2 Turning Power ON/OFF ...................................... 18
3.3 Setting Parameters ............................................ 21
3.4 Types of Screens ................................................ 26
3.5 Monitoring Screens ............................................ 27
3.6 Auxiliary Functions Screen (FUNCTION) ................ 31
3.6.1 List of Auxiliary Functions (FUNCTION) ....................... 31

3.6.2 Showing the Auxiliary Function Screen ....................... 33

3.6.3 [PARAMETER] Setting Group .................................. 34

3.6.4 [CONTROL] Setting Group .................................... 43

3.6.5 [SYSTEM] Setting Group ...................................... 45
3.6.6 [UTILITY] Setting Group ....................................... 47

3.6.7 [EDIT] Setting Group .......................................... 49

3.7 VP Functions ..................................................... 50
3.7.1 List of VP Functions .......................................... 50
iv SPD-40 SPD-40V
 Contents

3.7.2 Displaying the VP Functions .................................. 52

3.7.3 Product Information Group ................................... 52
3.7.4 Maintenance Information Group .............................. 54

3.7.5 Validation Support Group ..................................... 57

3.7.6 Calibration Support Group .................................... 60

3.7.7 Showing/setting CBM parameter (Calibration Support
Group) ......................................................... 67

3.8 Measuring in Single Wavelength Mode ............... 72

3.8.1 Setting the Measurement Range ............................. 72
3.8.2 Zeroing an Output to a Strip-Chart Recorder ............... 76
3.8.3 Setting [RESPONSE] ............................................ 77

3.9 Operation in Dual Wavelength Mode ................. 79

3.9.1 Selecting a Measurement Mode .............................. 79

3.9.2 Setting the Measurement Wavelength ....................... 79

3.9.3 Setting the Output Mode .................................... 79
3.9.4 Setting the Ratio Chromatogram Signal Output Value ...... 80
3.10 Operation in Spectrum Scanning Mode ............... 85
3.10.1 Setting Parameters Necessary to the Spectrum
Measurement .................................................. 85
3.10.2 Measurement Procedure ...................................... 88
3.10.3 Output of Spectrum Data .................................... 89
3.11 Creating Time Program ...................................... 92
3.11.1 Program Commands .......................................... 92
3.11.2 Setting Time Programming Parameters ....................... 93
3.11.3 Creating a Typical Time Program ............................. 94

3.11.4 Repeating the Time Program Steps [LOOP] .................. 95

3.11.5 Deleting Steps ................................................. 96

3.11.6 Starting a Time Program ...................................... 96

3.11.7 Stopping a Time Program .................................... 97

3.12 Connection with the System Controller ............... 98
3.12.1 Preparation .................................................... 98
3.12.2 Basic Parameters .............................................. 98
3.13 External Input/Output Terminals ......................... 99

SPD-40 SPD-40V v
 Contents

4 Maintenance 4.1 Periodic Inspection and Maintenance ................ 101

4.1.1 Prior to Inspection and Maintenance ....................... 101

4.1.2 List of Periodic Inspection and Maintenance ............... 102

4.1.3 Post-Inspection/Maintenance Leakage Check ................ 102

4.2 Removing/Installing the Flow Cell ..................... 103

4.2.1 Removing the Flow Cell .................................... 103
4.2.2 Installing the Flow Cell ..................................... 104
4.3 Flow Cell Inspection/Cleaning ............................ 105
4.4 Disassembling and Cleaning the Flow Cell ......... 108
4.5 Replacing a Flow Cell Part ............................... 113
4.6 Storing the Flow Cell ....................................... 116
4.7 Replacing the Lamp ......................................... 117
4.7.1 Removal of Top Cover and Radiating Fin Assembly ....... 119

4.7.2 Deuterium Lamp Replacement .............................. 120

4.7.3 Tungsten Lamp Replacement (for SPD-40V Only) .......... 124

4.8 Wavelength Accuracy Calibration ...................... 130

4.9 Absorbance Accuracy Calibration ....................... 132
4.10 Leak Sensor Calibration .................................... 136
4.11 Exterior Cleaning ............................................. 137

Technical 138
5 Information
5.1 Specifications ...................................................

5.1.1 Performance Specifications .................................. 138

5.1.2 Standard Flow Cell .......................................... 140

5.1.3 Detector Unit ................................................ 140

5.2 Consumable Parts/Maintenance
Parts/Replacement Parts .................................... 142
5.2.1 Consumable Parts ........................................... 142
5.2.2 Maintenance Parts ........................................... 142

Index ......................................................... 143

vi SPD-40 SPD-40V
1 Overview
The Shimadzu SPD-40 and SPD-40V are high-performance, multi-function ultra-violet
visible spectrophotometric detectors, for use in high performance liquid chromatograph. 1
The SPD-40V, with a D2 lamp and W lamp as light source, provides highly sensitive
analysis throughout the entire wavelength range.
Each instrument has 3 measurement modes - single wavelength, dual wavelength and
wavelength scanning.
The dual wavelength mode performs simultaneous measurement of two wavelengths, and
can provide chromatograms of both wavelengths, or a chromatogram of one wavelength
and one ratio chromatogram. In wavelength scanning mode, the absorbance spectrum
can be measured while the flow is stopped.

1.1 Features

n Excellent S/N ratio performance

An improved optical system and a high-order digital filter have enhanced the instrument's
signal to noise ratio.

n Advanced functions

The dual wavelength mode simultaneously provides chromatograms of two wavelengths

or ratio chromatograms, and the wavelength scanning mode provides absorbance spectra.
There is also a full set of Time Program functions.

n Improved stability in analysis through flow cell temperature control

Mobile phases have higher absorbances in shorter wavelengths than in longer ones, which
allows variation in the ambient temperature to affect the absorbance of the mobile phase
in the cell and leads to change in the baseline or peak areas. The instrument stabilizes
analysis by controlling the temperature of the flow cell.

n Easy replacement and inspection (maintenance) of consumables

Replacement of the lamps does not require troublesome optical axis adjustment. The
instrument enables users to monitor the total lamp usage time and to see when to
replace the lamps with a glance.
By using the absorbance spectrum of the built-in holmium oxide filter and the emission
line spectrum of the D2 (deuterium) lamp, the instrument checks the wavelength accuracy
across a range from ultraviolet light to visible light, which provides easy performance
verification.

SPD-40 SPD-40V 1
 1 Overview

n Cell ID and lamp ID functions

The built-in cell ID memory in the flow cell stores information on the type of the cell.
This enables the instrument to record the type of the cell used in an analysis in the
analysis data and provides better analysis traceability.
The lamps also have built-in ID memories and allow correct management of their
illumination time. The illumination time and ID numbers of the lamps used in an analysis
are automatically recorded in the analysis data, which enhances analysis traceability.

1.2 Component Parts

This instrument consists of the standard parts listed below. Check the parts against this
list after unpacking.

Part Name Part No. Q'ty Remark

Main Unit
－ 1
SPD-40/40V
It contains cautions for use
Safety Guideline (Booklet) 228-92326 1
regarding the instrument.
The paper based Instruction Manual
Instruction manual (CD-ROM) 228-92350-41 1 (228-92355) /System Guide
(228-92352) are available at a fee.
Syringe,1 ML 228-57028 1
Syringe needle 228-57027 1
Male nut PEEK 228-18565 2
ETFE tubing 228-18495-06 2
DRAIN TUBE 228-25162-03 1
Cap, hinge bracket 228-70057 1
REMOTE CABLE 228-28253-91 1
Drain OUT 228-42205 1
Drain CTO 228-42206 1
Straight tubing connector 228-28163 1
Drain adapter 228-42204 1
Coupling 1.6-0.8C 228-40998-10 1
Male nut 0.8MN-PEEK 228-46363 1

2 SPD-40 SPD-40V
 1.3 Optional Parts

1.3 Optional Parts

Optional parts available for this instrument are listed below.
For information about other optional parts listed below, contact your Shimadzu
1
representative. For features of each optional flow cell part, refer to "1.3.1 Optional Flow
Cell" P.3.

Part Name Part No. Remark

UHPLC cell 228-64724-42 A cell for high speed analysis.
Semi-micro cell 228-64725-42 A cell for semi-micro analysis.
A flow cell with the wetted part of inert (non-metal)
Inert cell 228-64728-42
materials.
A flow cell with the wetted part of inert (non-metal)
UHPLC Inert cell 228-74221-42
materials used for high speed analysis.
A flow cell with the wetted part of inert (non-metal)
Low Dispersion
228-74222-42 materials, which reduces sample dispersion, used for
Inert cell
semi-micro analysis.
Preparative cell A flow cell for fractionation. Users can change the
(variable path 228-64727-42 path length by replacing the provided spacer with
length) another.
Micro cell 228-64737-42 A flow cell for micro analysis.
High-pressure A flow cell with a high pressure resistance (40 MPa).
228-64726-42
resistant cell The cell can also be used for SFC analysis.
Conventional cell 228-68250-42 It is a flow cell used for conventional analysis.
Solvent recycling For recycling the mobile phase solvent. Controlled by
228-56808-42
valve kit the detector.
Signal cable 228-39306-91 It is necessary to output an analog signal.

1.3.1 Optional Flow Cell

For details on the specifications, refer to the instruction manual of each optional flow cell.

n Flow cell (Standard flow cell for SPD-40/SPD-40V) 228-62156-42

Item Features
Optical path length 10 mm
Capacity 12 µL
14 µL：I.D. 0.13mm, length 1040 mm (The length out of the
Inlet tube
cell is 315～340 mm.)
Outlet tube 8 µL：I.D. 0.25 mm, length 160 mm
Wetting part materials SUS316L, quartz, PFA, PEEK
Cell pressure tolerance 12 MPa
Cell temperature
Able
adjustment

SPD-40 SPD-40V 3
 1 Overview

n UHPLC cell 228-64724-42

Item Features
Optical path length 10 mm
Capacity 8 µL
4.3 µL：I.D. 0.1mm, length 550 mm (The length out of the cell
Inlet tube
is 315～340 mm.)
Outlet tube 2.1 µL：I.D. 0.13 mm, length 160 mm
Wetting part materials SUS316L, quartz, PFA, PEEK
Cell pressure tolerance 12 MPa
Cell temperature
Able
adjustment

n Semi-micro cell 228-64725-42

Item Features
Optical path length 5 mm
Capacity 2.5 µL
4.3 µL：I.D. 0.1 mm, length 550 mm (The length out of the cell
Inlet tube
is 315～340 mm.)
Outlet tube 2.1 µL：I.D. 0.13 mm, length 160 mm
Wetting part materials SUS316L, quartz, PFA, PEEK
Cell pressure tolerance 12 MPa
Cell temperature
Able
adjustment

n Inert cell 228-64728-42

Item Features
Optical path length 10 mm
Capacity 12 µL
32 µL：I.D. 0.25 mm, length 650 mm (The length out of the
Inlet tube
cell is 280～320 mm.)
Outlet tube 8 µL：I.D. 0.25 mm, length 160 mm
Wetting part materials PEEK, quartz, PFA
Cell pressure tolerance 8 MPa
Cell temperature
Able
adjustment

4 SPD-40 SPD-40V
 1.3 Optional Parts

n UHPLC Inert cell 228-74221-42

Item Features
Optical path length 10 mm 1
Capacity 8.0 µL
3.9 µL：I.D. 0.1 mm, length 500 mm (The length out of the cell
Inlet tube
is 315～340 mm.)
Outlet tube 2.0 µL：I.D. 0.125 mm, length 160 mm
Wetting part materials PEEK, quartz, PFA
Cell pressure tolerance 8 MPa
Cell temperature
Able
adjustment

n Low Dispersion Inert cell 228-74222-42

Item Features
Optical path length 5 mm
Capacity 2.5 µL
3.9 µL：I.D. 0.1 mm, length 500 mm (The length out of the cell
Inlet tube
is 315～340 mm.)
Outlet tube 2.0 µL：I.D. 0.125 mm, length 160 mm
Wetting part materials PEEK, quartz, PFA
Cell pressure tolerance 8 MPa
Cell temperature
Able
adjustment

n Preparative cell (variable path length) 228-64727-42

Item Features
Optical path length
0.1 mm 0.2 mm 0.5 mm (standard)
(variable)
Capacity
(variable depending on 0.8 µL 1.6 µL 4.0 µL
the path length)
201 µL：I.D. 0.8 mm, length 400 mm (The length out of the
Inlet tube
cell is 315～340 mm.)
Outlet tube 80 µL：I.D. 0.8 mm, length 160 mm
Wetting part materials SUS316L, quartz, PTFE, PCTFE, PEEK
Cell pressure tolerance 3 MPa
Cell temperature
Disable
adjustment

SPD-40 SPD-40V 5
 1 Overview

n Micro cell 228-64737-42

Item Features
Optical path length 3 mm
Capacity 0.21 µL
1.3 µL：I.D. 0.064 mm, length 400 mm (The length out of the
Inlet tube
cell is 315～340 mm.)
Outlet tube 2.1 µL：I.D. 0.13 mm, length 160 mm
Wetting part materials SUS316L, quartz, PTFE, PEEK
Cell pressure tolerance 8 MPa
Cell temperature
Disable
adjustment

n High-pressure resistant cell 228-64726-42

Item Features
Optical path length 10 mm
Capacity 12 µL
6.8 µL：I.D. 0.1 mm, length 870 mm (The length out of the cell
Inlet tube
is 315～340 mm.)
Outlet tube 8 µL：I.D. 0.25 mm, length 160 mm
Wetting part materials SUS 316L, quartz, PCTFE, PEEK
Cell pressure tolerance 40 MPa
Cell temperature
Disable
adjustment

6 SPD-40 SPD-40V
 1.3 Optional Parts

n Conventional cell 228-68250-42

Item Features
Optical path length 10 mm 1
Capacity 12 µL
43 µL：I.D. 0.25 mm, length 870 mm (The length out of the
Inlet tube
cell is 315～340 mm.)
Outlet tube 8 µL：I.D. 0.25 mm, length 160 mm
Wetting part materials SUS 316L, quartz, PFA, PEEK
Cell pressure tolerance 12 MPa
Cell temperature
Able
adjustment

n Selection of option cells

"Cell Selection" in the System Guide (228-92352)

SPD-40 SPD-40V 7
 2 Parts Identification and Function
2.1 Front

No. Name Description

Configure or operate the instrument with the
operation keys.
1 Operation panel
Touching the center part on the operation panel
displays the operation keys.
Open the cover to install/remove the flow cell, or to
2 Front cover attach tubing.
This cover must be closed during analysis.
Switches ON/OFF the power.
Pressing the switch turns on the power and pressing
it again turns off the power.
The switch is enabled only when the main power
3 (Power button) switch on the back is on.
Note that, with a system controller SCL-40 or CBM-40
connected, the switch does not illuminate and the
operation to the switch is ignored by default setting.
"[POWER BUTTON]" P.45

8 SPD-40 SPD-40V
 2.2 Behind Front Cover, Top, and Left Side

2.2 Behind Front Cover, Top, and Left Side

No. Name Description

Remove the cover to replace lamps. This cover must be
attached after lamp replacement.
1 Top cover For how to replace the part, see "4.7 Replacing the Lamp"
P.117. The instrument turns off the lamps automatically
when the cover is removed to ensure safety.
Flow cell body. The long tubing with a colored tube is the
inlet of the cell. (The tube color differs depending on the
type of cell. The standard cell has a red tube.)
2 Flow cell
Be sure to turn off the power of the
equipment when plugging or unplugging the
flow cell cable connector.

3 Cell mounting screws Loosen these screws to remove the cell.

Detects liquid leak.

If the leak sensor detects leak, an alarm goes off and a
message is displayed.

4 Leak sensor

The delivery pump connected via a system controller stops

operation.

Connect this outlet tubing to the drain tubing to flow

5 Cell outlet tubing
mobile phase used for analysis.
Cell inlet tubing
Connect to the column.
6 (marked with red
The length out of the cell is 315～340 mm.
cover)

SPD-40 SPD-40V 9
 2 Parts Identification and Function

No. Name Description

7 Coupling holder Fixes the coupling of the cell outlet tubing.

10 SPD-40 SPD-40V
 2.3 Back

2.3 Back

No. Name Description

1 [REMOTE] connector A connector for connecting the system controller.
A connector for connecting the external
equipment.
The rated value of the relay contact is 30 VDC,
2 External input/output terminals 0.2 A.
"3.13 External Input/Output Terminals"
P.99

A connector for connecting Chromatopac

3 [INTEGRATOR] connector
(integrator).
4 [RECORDER] connector A connector for connecting the recorder.
A connector for service personnel. Normally, this
5 [PC] connector
is not used.
6 Cooling fan Used for cooling the instrument interior.
It turns on/off the power to the instrument.
7 Main power switch
Normally keep it on.*1
8 Power cord connector A connector for connecting the power cord.

*1 Normally use (power button) of the operation panel or system controller to turn on/off the
power. If the system controller is connected and the main power switch is on, the user can use
(power button) of the system controller to turn on/off the power from the front of the instrument.
If the instrument is not used for a long time, turn off the main power switch.

SPD-40 SPD-40V 11
 2 Parts Identification and Function

2.4 Right Side and Bottom

No. Name Description

The instrument leaks drain from this port to the
1 Leakage drain outlet system component below.
System Guide "7.1 Installation"

Fixes the inside of the instrument to prevent the shock

2 Shipping screw during transportation.
Remove before installation.

12 SPD-40 SPD-40V
 2.5 Names and Functions of Display and Operation Area

2.5 Names and Functions of Display and Operation Area

This instrument is controlled through the keypad of the operation area. The display area
allows verification of the instrument status.
The operation area has two input modes: [Arrow key input mode] to enable screen
transfer, and [Numerical key input mode] to enable value input.
Pressing [Enter] switches between these input modes.

When turning off the main power switch on the back of the instrument after 2
changing the parameters via panel operation, be sure to turn OFF the power
with the power button at the front of the instrument, and turn off the main
power switch. Otherwise, some of the changed parameters may return to their
original values.

No. Name Description

1 Display Area Displays various screens and settings.
2 Link LED Illuminates when controlled by the system controller.
• Analysis ready: Illuminates in green
• Error: Illuminates in red
• Analysis in progress: Illuminates in blue
3 Status LED
• Preparation for analysis in progress: Illuminates in yellow
• Sleep mode: Illuminates in orange
• During update: Blinks in blue

SPD-40 SPD-40V 13
 2 Parts Identification and Function

No. Name Description

Switches ON/OFF the power.

• To turn the power ON:
Press and hold the power button for at least 3 seconds.
• To turn the power OFF:
Holding down the power button 3 seconds or more displays the
confirmation screen as shown in the figure. Holding it down
again 1 second turns off the power.
This button cannot be used by default setting when a system
(Power controller is connected.
4
Button)*1

If the system controller SCL-40 is connected, pressing the

power button of SCL-40 turns off the power of the entire
system.
"[POWER BUTTON]" P.45

Allows performing the baseline zero correction, starting a time

5 Direct Key
program, starting wavelength scan, and selecting a lamp mode.

*1 The button cannot be used if the instrument is linked with the system controller SCL-40. If SCL-40
is connected, pressing the power button of SCL-40 turns off the power of the entire system.
If CBM-40 or CBM-40lite is used, pressing the power button of the pump turns off the power of
the entire system.
Note, however, that the following will apply in the case of a system with multiple pumps.
- When using CBM-40lite: The system power can be turned off using the power button of the
pump where CBM-40lite is built in.
- When using CBM-40: The system power can be turned off using the power button of the pump
with the smallest optical link number.

14 SPD-40 SPD-40V
 2.5 Names and Functions of Display and Operation Area

2.5.1 Display Area

In this section the display on the initial screen is described.

No. Name Function

1 Absorbance Displays the absorbance (in the unit of mAU).
Displays the cell type.
2 Cell types "Screen to display the target temperature of cell
temperature control and a cell ID [CELL ID]" P.29

3 Set wavelength Displays the measurement wavelength (in the unit of nm).
Displays [D2] or [W], indicating D2/W source lamp is on. When
the deuterium (D2) lamp is on,
Lamp
4 illumination • D2: The deuterium (D2) lamp is on.
status • W: The tungsten (W) lamp is on.
• D2W: Both the deuterium and tungsten lamps are on.
Illuminates in the reverse polarity output mode (when the
5 Polarity polarity is set to "-").
"Polarity" P.23

Recycle valve
6 Illuminates when the recycle valve is draining liquid.
status
Recycle valve
7 Illuminates when the recycle valve is in operation.
status
8 LAMP -
Displays [SCAN] or [MARK]. For information to change the
function, see below.
9 SCAN/MARK
"Changing the function of the direct key 3 [DIRECT KEY3
SET]" P.47

10 RUN Highlighted during execution of a time program.

11 ZERO -

SPD-40 SPD-40V 15
 2 Parts Identification and Function

2.5.2 Operation Area

Use the keys on the front to operate or configure the instrument. When you touch the
key, the status LED lights up and accepts input.

No. Key Name Description

Performs the zero position adjustment of baseline.
1 [ZERO]*1 Zero Key "3.8.2 Zeroing an Output to a Strip-Chart
Recorder" P.76

Starts or stops a time program. (Key operation is

2 [RUN]*1 Run Key ignored if a time program is not configured.
"3.11.1 Program Commands" P.92

Use in the following cases. For information to change

the function, see below.
"Changing the function of the direct key 3
[DIRECT KEY3 SET]" P.47
[SCAN]*1 Scan Key
3 • SCAN: Activates the spectrum scanning function.
/[MARK] /Mark Key
"Scanning a spectrum [WAVE SCAN]" P.44

• MARK: Marks data being recorded on the recorder.

"3.11.1 Program Commands" P.92

Changes the lamp mode.

4 [LAMP] Lamp Key
"[LAMP]" P.36

This key is used for the following.

• To return from the display screen to the initial
screen.
5 [CE] Clear Key
• To clear the values input up to that time while
entering the values.
• To clear error message and cancel alarms.

16 SPD-40 SPD-40V
 2.5 Names and Functions of Display and Operation Area

No. Key Name Description

This key is used for the following.
• To delete input values.
6 [del] Delete Key
• To delete a line of the displayed time program while
creating it
7 [Enter] Enter Key Confirms input values of the setting items
[↑][→]
8 Arrow Keys Moves the cursor or switches the screen
[↓][←]
9 [·] to [9] Numeric Keys Inputs values for the setting items.
2
10 [-] Minus Key Used for inputting the parameters.

*1: These keys cannot be operated while initializing the instrument at startup.

SPD-40 SPD-40V 17
 3 Operation
3.1 Precautions

n Precautions before Operation

• The flow cell must be installed in the detector Fbefore turning the power switch ON.
If the power switch is turned on with no cell installed, home position will not be
detected and the wavelength will be set incorrectly.
If this occurs, turn the power switch OFF, install the cell and turn the power ON. The
cell may be removed after the initial screen is displayed.
• When highly sensitive analysis is necessary, take into account the time needed for the
baseline to stabilize and turn the lamp on ahead of time.
After it is turned on, the lamp requires roughly 1 hour to stabilize for optimal
performance.

n Precautions during Operation

Keep the front cover closed during analysis. In high-sensitivity analysis, opening or closing
the front cover will cause the baseline to fluctuate. The noise level may increase if the
front cover is open.

n Precautions after Operation

To prevent clogging of the flow cell:

Dusty or clogged flow cells are the most frequent cause of detector problems. After
analyzing a highly concentrated sample, flush the flow cell thoroughly, using plenty of
mobile phase. When a buffer solution is used as the mobile phase, wash the flow cell
with water after completing analysis. Buffer solutions crystallize upon evaporation, and
can clog the flow cell and tubing.

3.2 Turning Power ON/OFF

n Turning the Power ON

1 Press and hold (power button) for at least 3 seconds.

Power is supplied.

18 SPD-40 SPD-40V
 3.2 Turning Power ON/OFF

2 When the power is turned ON, the following sequence of events occurs:

1 Turns ON the power.

2 All LEDs on the operation area illuminate.

3 The instrument's memory is

automatically checked, and the
instrument name and the version
number are displayed for about 1
second.

4 The instrument checks the lamp switching function.

5 The lamps are preheated for about 30 seconds.

3
6 The instrument is initialized for
about 1 minute after turning on
the lamps.

7 The wavelength accuracy is

checked for about 20 seconds, by
using the absorbance spectrum of
the holmium oxide filter and the
656nm emission line of the D2
(deuterium) lamp.

8 If no error is detected, the

message on the right appears.
This screen remains on for several
seconds, followed by the initial
screen shown in step 9.

9 The initial screen shown on the

right appears. The instrument is
now in the initial state and
operation can be done. This is the
initial state.

n Error Display Examples

If an alarm sounds and a [WAVE CHECK NO GOOD] message is displayed on the screen:

1 No peak has been detected within

1nm of 656nm or 254nm. To stop
the alarm, press [CE].

2 Verify that the cell is installed

properly.
"4.2 Removing/Installing the Flow Cell" P.103

SPD-40 SPD-40V 19
 3 Operation

3 Check to be certain that no air bubbles have been introduced into the flow cell,
and that no mobile phase or sample which absorbs light in the vicinity of 254nm
or 656nm remains in the cell.
The wavelength calibration function [WAVE CALIB] and wavelength accuracy check
function [WAVE CHECK] measure the intensity of light transmitted through the
cell in the vicinity of the 656nm and 254nm
emission lines of the D2 (deuterium) lamp and mercury lamp. The instrument
operates based on these intensity values. Consequently, if a sample that absorbs
ultraviolet or visible light remains in the cell, or if
large air bubbles are introduced, the amount of transmitted light becomes
exceedingly small and the instrument will not operate correctly.
Operate using a flow cell that has been flushed or filled with a mobile phase
which does not absorb visible and UV light, or that has been purged of air or
nitrogen and dried thoroughly.

4 Execute wavelength calibration, referring to [WAVE CALIB] in Section 5.6, VP

Functions.
"[WAVE CALIB]" P.63

5 When the calibration is complete, the [WAVE CHECK] will be run automatically to
recheck the wavelength accuracy. If [WAVE CHECK NO GOOD] appears once again,
turn off the power of the instrument and contact the nearest Shimadzu
branch/sales office or agent.

If an alarm sounds and a [FATAL USER DATA] message is displayed on the screen:
Press [CE] to clear the alarm. When this message is displayed, the time program, along
with the [LAMBDA] (wavelength) and certain other parameters, will be initialized
(replaced with default values).

If any other error message is displayed:

System Guide "5.4 Error Message List"

n Turning the Power OFF

1 Press and hold (power button) for at least 3 seconds.

The status LED 2 blinks.

2 Press and hold the power button for another 1 second.

Power to the instrument is turned OFF.

20 SPD-40 SPD-40V
 3.3 Setting Parameters

3.3 Setting Parameters

The parameters that must be set prior to performing analysis are shown in the table
below, each along with its setting range, step (minimum interval), and default value.

Parameter Range Step Default Value

190 to 700
Wavelength 1 nm 254 nm
190 to 1000 (V model)
D2 and W ON
D2 ON
Lamp mode Switch D2 ON
W ON
OFF
Response
Response Switch 1.0 s
0 (No filtration), 50 to 20000 ms
3
Polarity + or - Switch +
Aux range 0.5, 1.0, 1.25, 2.0, 2.5 4.0 Switch 2.0
0: Outputs absorbance of ch1
1: Outputs absorbance of ch2 in
dual-wavelength measurement
2: Outputs a ratio chromatogram
signal to the recorder terminal in
dual-wavelength measurement 0: Outputs
Recorder mode Switch
3: Outputs a ratio chromatogram absorbance of ch1
signal to the integrator terminal
in dual-wavelength measurement
4: Outputs the temperature of
the temperature controlled cell.
(10 mV/100 °C)

Recorder output range 0 to 2.56 ( AU/10 mV ) 0.01 0.01

Ratio chromatogram
2 to 10000 1 10
setting
Ratio chromatogram
0.0001 to 1 0.0001 0.0001
threshold
Recycle valve switching
0 to 1.0000 AU 1 AU 0 AU
threshold
Recycle valve wait time 0 to 99.9 sec 0.1 s 2.0 s
Cell temperature 0 (OFF), 9 to 50 ℃ 1 ℃ 40 ℃
Cell temperature control
OFF or ON Switch ON
ready check
Cell temperature control
0 to 1.0 ℃ 0.1 ℃ 0.2 ℃
ready check range

Parameters are set in the [PARAMETER] setting group of the auxiliary functions screen
(FUNCTION).
"3.6 Auxiliary Functions Screen (FUNCTION)" P.31
"3.6.3 [PARAMETER] Setting Group" P.34

SPD-40 SPD-40V 21
 3 Operation

n Wavelength

Specify the wavelength to be measured. The wavelength2 can set only in the the
dual-wavelength measurement mode. The SPD-40V uses a D2 lamp and a W lamp as the
light source that provides a 190 to 1000 nm wavelength range absorption spectrum. To
measure only the ultraviolet region, turn on the D2 lamp only.

Model (Lamp) Wavelength Setting Rrange

SPD-40 190 nm to 700 nm
D2 lamp 190 nm to 370 nm
SPD-40V W lamp 371 nm to 1000 nm
D2 and W lamps 190 nm to 1000 nm

Valid setting ranges

The wavelength setting range shown on the bottom line of the screen varies,
depending on the model and the lamp used. Values outside the setting range
will not be accepted.

See below for information about the the single wavelength mode.
• "Setting Wavelength1 [LAMBDA 1]" P.34

• "3.8 Measuring in Single Wavelength Mode" P.72

See below for information about the dual-wavelength measurement mode and the
wavelength scanning mode.
• "Setting the mode to dual-wavelength measurement mode [WAVE MODE]" P.35

• "Setting Wavelength2 [LAMBDA 2]" P.35

• "3.9 Operation in Dual Wavelength Mode" P.79

• "3.10 Operation in Spectrum Scanning Mode" P.85

• The detector has two of signal output connectors: [RECORDER] connector and
[INTEGRATOR] (integrating data processor) connector.
A Chromatopac or variable range recorder can be connected to either of the
two connectors, but a Chromatopac should normally be connected to the
[INTEGRATOR] connector. A fixed range recorder must be connected to the
[RECORDER] connector so that its recording range can be adjusted using the
instrument's recorder range setting function. If a Chromatopac is connect to
the [RECORDER] connector, the Chromatopac's input full scale will be
equivalent to 100 times the absorbance value set for the recorder range.
Accordingly, the absorbance value set for the range should be about 1/80 of
the expected maximum peak absorbance.
• When the wavelength is changed:
The [RECORDER] connector signal output level changes to 0V for 4 seconds,
then returns to the previous signal level. This results in a mark at the point
of wavelength change on the chromatogram. However, since this could
interfere with area calculations, the [INTEGRATOR] connector output is
designed to maintain its signal level unchanged during the 4 second time
period.

22 SPD-40 SPD-40V
 3.3 Setting Parameters

• "When a Chromatopac (Integrator) Is Used As a Recorder:" P.72

• "When a Strip-Chart Recorder Is Used:" P.74

• "When a Chromatopac (Integrator) is connected to the [RECORDER] Connector" P.75

n Response

How responsive the digital filter is influences the S/N and responsiveness of the
instrument.
Noise increases as the filter's response becomes faster, and decreases as it becomes slowly.
A quicker response of the filter results in a larger noise. A slower response value leads
to a smaller noise.
"[RESPONSE]" P.35

n Polarity 3
Setting "-" to polarity inverts the polarity of absorbance of recorder output terminal and
integrator output terminal from positive (negative) to negative (positive). Normally use
"+" for polarity. If the absorbance of the mobile phase is higher than the absorbance
of the component targeted for analysis (using the indirect absorbance detection method),
set the polarity to "-".
"Setting the [POLARITY]" P.37

is displayed in the lower right portion of the MONITOR initial screen

when the polarity is "-".

n AUX range

Set this parameter when using the Chromatopac as a recorder.

• "When a Chromatopac (Integrator) Is Used As a Recorder:" P.72

• "Setting integrator output range[AUX RANGE]" P.37

SPD-40 SPD-40V 23
 3 Operation

n Recorder setting

Recorder mode
Set the mode of output to the recorder.
The modes available are as follows.

No. Mode Description

0 Ch1 absorbance output Select this to output absorbance of Ch1.
1 Ch2 absorbance output Select this to output absorbance of Ch2.
Select this to record a ratio
Ratio chromatogram signal output to
2 chromatogram signal by using a
the recorder
recorder.
Select this to record a ratio
Ratio chromatogram signal output to
3 chromatogram signal by using
the integrator terminal
Chromatopac.
Outputs the temperature of the
4 Cell temperature output temperature controlled cell. (10mV/100
°C)

• "When a Strip-Chart Recorder Is Used:" P.74

• "[REC MODE]" P.38

Recorder output range

Set the recorder output range.
"[RANGE]" P.37

Ratio chromatogram setting

Set the full output scale for outputting ratio chromatograms.
"[RATIO RANGE]" P.39

Ratio chromatogram threshold

Set the threshold for outputting ratio chromatograms. The data are outputted when the
absorbances of both wavelengths exceed this value.
"[RATIO THR]" P.40

24 SPD-40 SPD-40V
 3.3 Setting Parameters

n Recycle valve

Recycle valve mode

Setting "[SV LEVEL]" P.40 automatically changes the following modes according to the
threshold values.
• Recycle
• Drain
According to the recycle valve mode, the following symbols are displayed on the lower
right of the initial screen on the monitor.

Display Description Reference

Displayed when the recycle valve is in the recycle position. P.40

3
Displayed when the recycle valve is in the drain position. P.40

Delay Time and Threshold

Set the delay time for the solvent recycle valve to switch to the mode when absorbance
decreases below the threshold.
"[SV DELAY TIME]" P.40

n Controlled Cell Temperature

The instrument controls the temperature of the flow cell and thus mitigates the impact
of ambient temperature variation on the baseline. The temperature control is effective
when the mobile phase has a high absorbance or when the sample has a strong
temperature dependence.

If the temperature of the column is higher than the set temperature of the flow
cell, the heat of the mobile phase is transferred and the temperature of the
flow cell may not drop to the set temperature. In this case, increase the set
temperature of the flow cell.
If the flow cell temperature exceeds the upper limit of 50 ° C, turn off the cell
temperature control.

• To deactivate the temperature control: Enter "0" in the temperature setting.

Ready Check
The ready check ensures that analysis starts after heat balance is established at
temperature-controlled locations.
The ready check is available for the flow cell and polychromator.
"Setting the range where the cell temperature control is considered ready [CELL READY]"
P.47

SPD-40 SPD-40V 25
 3 Operation

3.4 Types of Screens

Turning ON the power displays the initial screen.
By pressing the [↓] and [→] keys on the initial screen, the screen can be switched from
the initial screen to one of three screens below:

Displays Screen Description

View the status of the instrument.
Monitor screen
"3.5 Monitoring Screens" P.27

Configure various parameter settings and perform operations.

Auxiliary functions screen
"3.6 Auxiliary Functions Screen (FUNCTION)" P.31

Support the validation of the instrument with functions for

VP function screen viewing and checking instrument information.
"3.7 VP Functions" P.50

Pressing [→] on the initial screen displays the screen to select the auxiliary functions
screen [FUNCTION] or VP functions screen [VP]. Move the cursor to the desired option
with [↓] or [↑] and press [→] to display the screen. Press [CE] to return to the initial
screen.

26 SPD-40 SPD-40V
 3.5 Monitoring Screens

3.5 Monitoring Screens

This section describes the monitoring
screens.
Press [↓] key on the initial screen to show
the various types of information.
Initial Screen

Name Description Reference

In the single-wavelength measurement mode:
The screen displays an absorbance, wavelength setting,
lamp illumination status, recycle valve status, and cell type.

The recycle valve status is displayed only

P.28
3
Initial Screen when a recycle valve is connected.
P.28

In the dual-wavelength measurement mode:

The screen displays an absorbance, wavelength setting,
and lamp illumination status for each wavelength to be
measured.
Displays the output levels of the reference light beam and
CH1: SMP/REF P.28
sample light beam*1 of the wavelength to be measured 1.
Displays the amounts of the reference light and sample
light*1 of the wavelength to be measured 2.
CH2: SMP/REF P.29
Displayed only in the dual-wavelength
measurement mode.

Displays the target temperature of cell temperature

control and a cell ID.
CELL ID P.29
The screen displays [CELL Disconnected] when
no cell is connected.

D2 LAMP USED Displays the usage time and the estimated replacement
P.29
TIME time of the D2 lamp.
W LAMP USED Displays the usage time and the estimated replacement
P.29
TIME*2 time of the W lamp.
TOTAL OP TIME Displays the total operation time. P.30
Displays the time that elapsed after a time program was
PLOG TIME P.30
started.

*1 Sample side: Refers to the light passing through the mobile phase and specimen.*1 Sample side:
Refers to the light passing through the mobile phase and specimen.
Reference side: Refers to the light used as the reference light without passing through the mobile
phase.
*2 It is only displayed in the SPD-40AV.

SPD-40 SPD-40V 27
 3 Operation

n Initial Screen (in the Single-wavelength Mode)

Starting from the left, the first row shows the current absorbance, wavelength to be
measured, and lamp illumination status.
Starting from the left, the second row
shows the cell type and recycle valve
status.

Displays the type of the connected cell. The following lists the types of available cells.

Type Cell Name

STD Standard cell
UHPLC UHPLC cell
SEMI-M Semi-micro cell
INERT Inert cell
PREP Fractionation cell
MICRO Micro cell
[Link] High-pressure resistant cell
CONV. Conventional cell

Furthermore, according to the conditions of the polarity/recycle valve, the following

symbols are displayed on the lower right of the screen.

Display Description Reference

Displayed when the polarity is "-". P.37

Displayed when the recycle valve is in the recycle position. P.40

Displayed when the recycle valve is in the drain position. P.40

n Initial Screen (in the Dual-wavelength Mode)

Starting from the left, the first row shows the absorbance, wavelength to be measured,
and lamp illumination status of the wavelength to be measured 1.
Starting from the left, the second row
shows the absorbance and wavelength to
be measured of the wavelength to be
measured 2.

n Screen to display the output level of the light beam of the wavelength to be measured
1 [CH1: SMP/REF]

The first row displays the output level of the sample light beam of the wavelength to
be measured 1.

28 SPD-40 SPD-40V
 3.5 Monitoring Screens

The second row displays the output level

of the reference light beam of the
wavelength to be measured 1.

n Screen to display the output level of the light beam of the wavelength to be measured
2 [CH2: SMP/REF]

The first row displays the lamp illumination status and the output level of the sample
light beam of the wavelength to be measured 2.
The second row displays the output level
of the reference light beam of the
wavelength to be measured 2.

[CH2: SMP/REF] is only displayed 3

in the dual-wavelength
measurement mode,.

n Screen to display the target temperature of cell temperature control and a cell ID [CELL
ID]

The first line indicates cell type and controlled temperature of the cell starting from the
left.
The second row displays a cell ID.

The screen displays [CELL

Disconnected] when no cell is
connected.

n Screen to display the D2 lamp illumination time [D2 LAMP USED TIME]

Displays the usage time and the estimated

replacement time of the D2 lamp.
To change the D2 lamp usage time,
see "[D2 LAMP USED TIME]" P.54.

n Screen to display the W lamp illumination time [W LAMP USED TIME]

Displays the usage time and the estimated

replacement time of the W lamp.
To change the W lamp usage time,
see "[W LAMP USED TIME]" P.54.

The screen is displayed on SPD-40V only.

SPD-40 SPD-40V 29
 3 Operation

n Screen to display the total operation time [TOTAL OP TIME]

Displays the total operation time of the

instrument.

n Screen to display the time that elapsed after a time program was started [PROG TIME]

Displays the time that elapsed after a time

program was started.

The screen displays a maximum

of 999.99 h.

"3.11 Creating Time Program" P.92

30 SPD-40 SPD-40V
 3.6 Auxiliary Functions Screen (FUNCTION)

3.6 Auxiliary Functions Screen (FUNCTION)

The auxiliary functions screen has five setting groups. Switch the groups with [↑] or [↓]
and press [→] to enter the screen of the desired setting group. Press [CE] to return to
the initial screen.
"3.6.1 List of Auxiliary Functions (FUNCTION)" P.31

Setting Group Name Description

PARAMETER The group allows the user to set analysis parameters.
CONTROL The group enables specific operation.
The group allows the user to set parameters related to the
SYSTEM system such as parameters of connection with external
equipment.
The group allows the user to set parameters not related to the
3
UTILITY
analysis result such as parameters of display and buzzer.
EDIT Time programs can be edited.

3.6.1 List of Auxiliary Functions (FUNCTION)

Moves the cursor to this group and press [→] to enter the screen of the desired setting
command.
Operation in the table head shows the types of operation described below.
• Display: Check the monitor.
• [Enter]: Press [Enter] to activate the function.
• Numeric keypad: After pressing [Enter] to display Numeric keypad, press [0] - [9] to
enter a value and press [Enter] to accept the value.

n [PARAMETER] settings group

Command Operation Function Ref.

LAMBDA 1 Numeric keypad Sets wavelength. P.34
Sets ch2 wavelength in the
LAMBDA 2 Numeric keypad dual-wavelength measurement P.35
mode.
Switches between
WAVE MODE Numeric keypad single-wavelength mode and P.34
dual-wavelength mode.
RESPONSE Numeric keypad Sets [RESPONSE]. P.35
LAMP Numeric keypad Sets lamp ON/OFF. P.36
POLARITY Numeric keypad Sets the output polarity. P.37
AUX RANGE Numeric keypad Sets integrator output range. P.37
RANGE Numeric keypad Sets recorder output range. P.37
REC MODE Numeric keypad Sets recorder output mode. P.38

SPD-40 SPD-40V 31
 3 Operation

Command Operation Function Ref.

Sets baseline offset value for
BL OFFSET ITG Numeric keypad integrator zero position P.38
adjustment.
Sets baseline offset value for
BL OFFSET REC Numeric keypad P.38
recorder zero position adjustment.
Sets temperature for flow cell
CELL TEMP Numeric keypad P.39
temperature adjustment.
Sets external event 1 terminal
EVENT1 Numeric keypad P.39
status.
Sets external event 2 terminal
EVENT2 Numeric keypad P.39
status.
Sets range in ratio chromatogram
RATIO RANGE Numeric keypad P.39
output.
Sets threshold value in ratio
RATIO THR Numeric keypad P.40
chromatogram output.
Sets threshold level for switching of
SV LEVEL Numeric keypad P.40
solvent recycling valve.
Sets solvent recycling valve delay
SV DELAY TIME Numeric keypad P.40
time.
W LAMP POWER*1 Numeric keypad Sets intensity of tungsten lamp. P.41
Sets the file number where scanned
SCAN FILE Numeric keypad P.41
data is to be stored.
SCAN BEGIN Numeric keypad Sets the scan start wavelength. P.41
SCAN END Numeric keypad Sets the scan end wavelength. P.41
SCAN STEP Numeric keypad Sets the scanning wavelength step. P.42
Sets the output speed of spectrum
PLOT SPEED Numeric keypad P.42
data to recorder.

*1 The W (tungsten) lamp is present in the SPD-40V only.

n [CONTROL] settings group

Command Operation Function Page

WAVE CHECK [Enter] Checks wavelength accuracy. P.43
WAVE SCAN [Enter] Scans a spectrum. P.44
SPC PLOT [Enter] Outputs spectrum data to recorder. P.44

n [SYSTEM] setting group

Command Operation Function Page

Selects independent operation or
LOCAL MODE Numeric keypad P.45
control via system controller.
Sets address of the instrument for
LINK ADDRESS Numeric keypad P.45
ontrol via system controller.

32 SPD-40 SPD-40V
 3.6 Auxiliary Functions Screen (FUNCTION)

Command Operation Function Page

Sets whether to enable individual
operation from the power button
POWER BUTTON Numeric keypad on the front of the instrument P.45
even when connected to the system
controller SCL-40/CBM-40.
Sets the function of the event
SELECT EVENT1 Numeric keypad P.46
terminal 1.
Sets the function of the event
SELECT EVENT2 Numeric keypad P.46
terminal 2.
Sets the range where the cell
CELL READY RNG Numeric keypad temperature control is considered P.47
ready.
Sets the cell temperature control
CELL READY Numeric keypad P.47
ready check function. 3
Changes the function of the direct
DIRECT KEY3 SET Numeric keypad P.47
key 3.

n [UTILITY] setting group

Command Operation Function Page

KEY CLOSE [Enter] Locks key input. P.48
BRIGHTNESS Numeric keypad Sets display brightness. P.48
BEEP MODE Numeric keypad Sets the operation of buzzer. P.48
VOLUME Numeric keypad Sets the buzzer volume. P.48
The operation panel turns off after
DISP OFF TIME Numeric keypad P.48
the time set here elapses.
Specifies whether to display the
DIRECT KEY Numeric keypad confirmation screen when the P.49
direct key is operated.

n [EDIT] setting group

Use this group to create a time program. For details, see "3.11 Creating Time Program"
P.92.

3.6.2 Showing the Auxiliary Function Screen

1 Press [CE].
The initial screen is displayed.

SPD-40 SPD-40V 33
 3 Operation

2 Press [→] twice.

The auxiliary function groups appear.

3 Select a desired group and press [→].

The first item in the selected group
appears. (Example: In the [PARAMETER]
setting group)

4 Press [↑] or [↓] to select a setting screen.

Press [CE] to return to the initial screen.

3.6.3 [PARAMETER] Setting Group

This group is for setting detector parameters.

n Setting Wavelength1 [LAMBDA 1]

Set the detector to the measurement wavelength in the single wavelength mode, or to
the measurement wavelength1 in the dual-wavelength measurement mode.
Press [Enter] to display the numeric keypad
and then input a wavelength to measure
before pressing [Enter].

Model (Lamp) Wavelength Setting Rrange

SPD-40 190 nm to 700 nm
D2 lamp 190 nm to 370 nm
SPD-40V W lamp 371 nm to 1000 nm
D2 and W lamps 190 nm to 1000 nm

in the dual-wavelength measurement mode, the available setting range for [LAMBDA 1]
and that for [LAMBDA 2] are the same.
"Setting Wavelength2 [LAMBDA 2]" P.35

34 SPD-40 SPD-40V
 3.6 Auxiliary Functions Screen (FUNCTION)

n Setting Wavelength2 [LAMBDA 2]

Set the detector to the in the

dual-wavelength measurement mode.
See below for switching the
dual-wavelength measurement mode.
"Setting the mode to dual-wavelength measurement mode [WAVE MODE]" P.35

Press [Enter] to display the numeric keypad and then input a wavelength to measure
before pressing [Enter].

[LAMBDA 2] is displayed only in the dual-wavelength measurement mode.

Model (Lamp) Wavelength Setting Rrange

3
190 nm to 370 nm or
SPD-40
371 nm to 700 nm
D2 lamp 190 nm to 370 nm
371 nm to 700 nm or
W lamp
701 nm to 1000 nm
SPD-40V
190 nm to 370 nm or
D2 and W lamps 371 nm to 700 nm or
701 nm to 1000 nm

If a wavelength outside the wavelength setting range is set, [SET ANOTHER λ]

is displayed and the same wavelength is applied for measurement wavelength 1
and measurement wavelength 2.

n Setting the mode to dual-wavelength measurement mode [WAVE MODE]

This function switches the mode between

single-wavelength mode and
dual-wavelength mode.
Press [Enter] to display the numeric
keypad, then press [1] to switch the mode to single-wavelength chromatogram mode or
press [2] to switch the mode to dual-wavelength mode before pressing [Enter].
The value displayed in the upper right is "1" in single-wavelength mode while the value
is "2" in dual-wavelength mode.

n [RESPONSE]

Set the response.

Press [Enter] to display the numeric
keypad, then input the values and press
[Enter].
• When connected with CBM: Can be set to any of the following three levels: high speed,
standard, or low speed. The correspondence of the set value and response is shown
below.

SPD-40 SPD-40V 35
 3 Operation

Set
Response
Value
1 Fast (Sampling Ratio ´ 1)

2 Standard (Sampling Ratio ´ 2)

3 Slow (Sampling Ratio ´ 4)

• When not connected with CBM or in the SPD-20A compatible mode:

Can be set to any of the following 11 levels: 0 to 10. The following table shows the
time constant of the conventional analog filter corresponding to the change in
responsiveness made by the set value of [RESPONSE].

Set Value Corresponding time constant of analog filter

0 No filtering
1 0.05 sec (FAST)
2 0.1 sec
3 0.5 sec (STD)
4 1.0 sec
5 1.5 sec (SLOW)
6 3.0 sec
7 6.0 sec
8 8.0 sec
9 10.0 sec
10 2.0 sec

"3.8.3 Setting [RESPONSE]" P.77

n [LAMP]

Turns D lamp and W lamp*1 ON/OFF.

Select a set value by numeric keypad, then
press [Enter].

Set Value Lamp Status

0 OFF
1 D2 (deuterium) lamp ON
2*1 W (tungsten) lamp ON
D2 (deuterium) lamp ON
3*1
W (tungsten) lamp ON

*1 SPD-40V only

36 SPD-40 SPD-40V
 3.6 Auxiliary Functions Screen (FUNCTION)

n Setting integrator output range[AUX RANGE]

Set this parameter when using the

Chromatopac as a recorder.
"When a Chromatopac (Integrator) Is
Used As a Recorder:" P.72

Press [Enter] to display the numeric keypad, then select the set value and press [Enter].
Set
Range (AU/V)
Value
1 0.5
2 1
3 2
4 4
3
5 1.25
6 2.5

n Setting the [POLARITY]

Setting “-” to polarity inverts the polarity

of absorbance of the recorder output
terminal and integrator output terminal
from positive (negative) to negative
(positive). Usually set as “+”.
Press [Enter] to display the numeric keypad, then then set the value to "0" or "1" and
press [Enter].

is displayed in the lower right portion of the MONITOR initial screen when
the polarity is "-".

n [RANGE]

Sets the recorder output range.

"When a Strip-Chart Recorder Is
Used:" P.74

Select a range by numeric keypad, then

press [Enter].

Set Range (AU/10 mV)

0 to 2.5600

When [0] is selected, the recorder output is set to 0mV.

"3.8.2 Zeroing an Output to a Strip-Chart Recorder" P.76

SPD-40 SPD-40V 37
 3 Operation

n [REC MODE]

Sets the [RECORDER] connector output

mode.
Select a set value by numeric keypad, then
press [Enter].

Set Value Output Mode

0 Ch1 absorbance output
1 in the dual-wavelength measurement mode, Ch2 absorbance output
in the dual-wavelength measurement mode, ratio chromatogram signal
2
output to [RECORDER] connector
in the dual-wavelength measurement mode, ratio chromatogram signal
3
output to [INTEGRATOR] connector
4 Flow cell's temperature reading output (10 mV/100 °C)

When [1], [2] or [3] is selected, the ch1 absorbance is output in single wavelength mode.

n [BL OFFFSET ITG]

Sets the baseline offset value for the

integrator output.
Press [Enter] to display the numeric
keypad, then input the set value and press
[Enter].
This is the offset value for the integrator output connector.
Set Range (mV)
-2 to 250

n [BL OFFSET REC]

Sets the baseline offset value for the

recorder output.
Press [zero] to fix the recorder output to
the value specified here. This is the offset
value for the recorder output connector.
Select a set value by numeric keypad, then press [Enter].

Set Range (mV)

-2 to 250

38 SPD-40 SPD-40V
 3.6 Auxiliary Functions Screen (FUNCTION)

n [CELL TEMP]

Sets the temperature of the temperature

controlled flow cell.
Press [Enter] to display the numeric
keypad, then input the values and press
[Enter].

This parameter is displayed only when a temperature-controllable cell is

recognized.

Set Range (°C)

0: temperature adjustment OFF, or 9 to 50
3
It is possible to set the temperature setting as low as 9 (°C), but it should be
set at a value at least 5 (°C) or more above room temperature. Setting it at
some other value can result in a [LOW SET TEMP] error.

n [EVENT1], [EVENT2]

Sets the relays ON (close) / OFF (open)

accessed by the EVENT output terminals on
the back of the instrument.
Press [Enter] to display the numeric
keypad, then input the set value and press
[Enter].
"3.13 External Input/Output
Terminals" P.99

n [RATIO RANGE]

Sets the full-scale output for a ratio

chromatogram.
Press [Enter] to display the numeric
keypad, then input the set value and press
[Enter].

Set Range
2 to 10000

"3.9.4 Setting the Ratio Chromatogram Signal Output Value" P.80

SPD-40 SPD-40V 39
 3 Operation

n [RATIO THR]

Sets the threshold level for a ratio

chromatogram.
A ratio chromatogram is output only when
the absorbance signals for both
wavelengths exceed this value.
Press [Enter] to display the numeric keypad, then input the set value and press [Enter].

Set Range (AU)

0.0001 to 1.0

"Setting [RT THR] (Ratio Threshold)" P.82

n [SV LEVEL]

Sets the threshold level for switching the

solvent recycling valve.
When the absorbance signal exceeds this
value, the solvent recycling valve is
switched to the liquid draining (waste)
position.

This item is displayed only when the recycle valve is connected.

Set Range (AU) Step

0 to 1 0.0001

• If the solvent recycling valve is not used, or to set the valve permanently to the
recycling position, set [0] for this function.
• To set the valve permanently to the waste position, set [1].
Press [Enter] to display the numeric keypad, then input the set value and press [Enter].
For the piping of recycle valve, refer to the Instruction Manual of the recycle valve
(228-73490).

n [SV DELAY TIME]

This sets the delay time for switching the

solvent recycle valve back to the recycling
position, after the absorbance signal has
fallen below the [SV LEVEL].

Set Range (sec)

0 to 99.9

This item is displayed only when the recycle valve is connected.

40 SPD-40 SPD-40V
 3.6 Auxiliary Functions Screen (FUNCTION)

n [W LAMP POWER]

This function adjusts the intensity of the

W (tungsten) lamp.
Range 0-9 (9 is maximum intensity).

[W LAMP POWER] is displayed

only in SPD-40V.

n [SCAN FILE]

Specifies the file containing data to be

scanned.
Press [Enter] to display the numeric 3
keypad, then input the file number and
press [Enter].
Enter a file number from 0 to 2. The number 0 is used for background data while 1 and
2 are used for scanned data.
"3.10 Operation in Spectrum Scanning Mode" P.85

n [SCAN BEGIN]

Sets scan starting wavelength.

Press [Enter] to display the numeric
keypad, then input the scan starting
wavelength and press [Enter].

The wavelength settings range displayed on the bottom line differs by model
according to the table of "Start/End Wavelength Setting Range".

"3.10 Operation in Spectrum Scanning Mode" P.85

n [SCAN END]

Sets scan ending wavelength.

Press [Enter] to display the numeric
keypad, then input the scan ending
wavelength and press [Enter].

The wavelength settings range displayed on the bottom line differs by model or
lamp according to the table of "Start/End Wavelength Setting Range".

"3.10 Operation in Spectrum Scanning Mode" P.85

SPD-40 SPD-40V 41
 3 Operation

Start/End Wavelength Setting Range

Wavelength Setting Range
Model (Lamp) End wavelength
Start wavelength
Operation panel Labsolutions
(Start wavelength + 1)～ （Start wavelength +
190～369 nm
700 nm 1）～370 nm
SPD-40
(Start wavelength + 1)～ （Start wavelength +
371～699 nm
700 nm 1）～700 nm
（Start wavelength + （Start wavelength +
D2 lamp 190～369 nm
1）～370 nm 1）～370 nm
（Start wavelength + （Start wavelength +
371～699 nm
1）～1000 nm 1）～700 nm
W lamp
（Start wavelength + （Start wavelength +
701～999 nm
1）～1000 nm 1）～1000 nm
SPD-40V
（Start wavelength + （Start wavelength +
190～369 nm
1）～1000 nm 1）～370 nm
D2 and W （Start wavelength + （Start wavelength +
371～699 nm
lamps 1）～1000 nm 1）～700 nm
（Start wavelength + （Start wavelength +
701～999 nm
1）～1000 nm 1）～1000 nm

n [SCAN STEP]

Scan step controls the speed of wavelength

scanning.
Press [Enter] to display the numeric
keypad, then input the scan step and press
[Enter].

Set Range (nm)

1 to 5

"3.10 Operation in Spectrum Scanning Mode" P.85

n [PLOT SPEED]

Sets the speed at which spectrum data is

sent to the recorder.
Press [Enter] to display the numeric
keypad, then selecting the set value and
press [Enter].
"3.10 Operation in Spectrum Scanning Mode" P.85

Set Value Output speed

1 1 nm/sec
2 3 nm/sec
3 10 nm/sec

42 SPD-40 SPD-40V
 3.6 Auxiliary Functions Screen (FUNCTION)

3.6.4 [CONTROL] Setting Group

This is the group for system control.

n Checking the wavelength accuracy [WAVE CHECK]

This function checks the wavelength accuracy of the instrument.

Press [Enter].

[CHECKING 241nm] or [CHECKING 656nm]

is displayed while the check is in progress.
3
After the result is displayed, the wavelength accuracy is checked.
When the wavelength is confirmed to be accurate, [WAVE CHECK GOOD] is displayed.
The figure shows that the wavelength accuracy is 0.3 for the absorbance peak of the
holmium filter*1 at near 241 nm and that the wavelength accuracy is 0.1 nm for the
emission line of the D2 lamp at 656.1 nm.
*1: The peak absorption wavelength
ranges from 240.4 to 241.7 nm depending
on the instrument (holmium filter).

When the wavelength is confirmed to be inaccurate, [WAVE CHECK NOGOOD]

is displayed. If this happens, calibrate the wavelength accuracy by using [WAVE
CALIB].

"4.8 Wavelength Accuracy

Calibration" P.130

If the error is not resolved, refer

to the following.

System Guide "5.4 Error Message List"

How to measure the wavelength accuracy

SPD-40/SPD-40V use zero-order light (0 nm) and the emission line (656.1 nm) of a
deuterium lamp (D2 lamp) for calibrating the wavelength. The wavelength accuracy can
be secured by using those wavelengths as the reference for adjustments.
The technique to calibrate wavelengths using the emission line of a deuterium lamp (D2
lamp) is stipulated in the JIS standard as follows.

JIS K0115 General rules for molecular absorptiometric analysis

• 5.2.1 Calibration of wavelength scales (2) Technique to use an emission line of a lamp
Measure the light intensity of a low-pressure mercury lamp or a deuterium lamp to
determine the wavelength at the maximum intensity. Compare this wavelength with
that of the emission line for calibration.

SPD-40 SPD-40V 43
 3 Operation

(Supplementary explanation)
The evaluation of a test is performed through a direct comparison with the emission
line specific to the lamp (universal physical constant). The traceability of universal
physical constants is described in the following literature.
（Recommend Practice）
Medical Products And Pharmaceutical Calibration Control System July 10, 1986, National
Conference of Standards Laboratories
"5.2.2 Additional Traceability documentation （as required）
The use of calibration standards such as natural physical constants, derived standards,
standard reference materials, or internal standards which are not traceable to national
standards, will require adequate documentation to describe the validity of the
calibration standards used."

n Scanning a spectrum [WAVE SCAN]

This function scans a spectrum in a specific

wavelength range.

Pressing [Enter] starts scanning a spectrum.

During scan, the screen displays the
wavelength being scanned in the lower
right.

When the scan is completed, the screen

displays [Scan End].

Press the CE key to stop

scanning a spectrum.

n [SPC PLOT]

Outputs spectrum data to the recorder.

Press [Enter].
While data output is in progress, the
screen displays [Plotting...].

When output is completed, the screen

displays [Plot End].

44 SPD-40 SPD-40V
 3.6 Auxiliary Functions Screen (FUNCTION)

If there are no data to be outputted, the

screen displays [DATA NOT EXIST].

To stop data output to the recorder, press [CE] again while [Plotting...] is displayed.

3.6.5 [SYSTEM] Setting Group

This is the group for system settings.

n [LOCAL]
3
Sets whether this instrument is operated by system controller or the instrument operates
independently when system controller is connected.
The screen displays the current setting in
the upper right.
Press [Enter] to display the numeric
keypad, input the set value and press
[Enter].
Set Value Mode Function
0 Remote Operate via system controller (initial setting)
1 Local Operate independently (in local mode)

n [LINK ADDRESS]

Sets the address (channel number) when

this instrument is connected to system
controller.
Press [Enter] to display the numeric
keypad, input the address No. press [Enter].
"3.12 Connection with the System Controller" P.98.

n [POWER BUTTON]

Sets the power button on the front of the

instrument.
Input the value with the numeric keypad
and press [Enter].

Set Value Function

Operation of the power button from the instrument is disabled according to
0
the control of the system controller only. (Initial value)

SPD-40 SPD-40V 45
 3 Operation

Set Value Function

In addition to being controlled by the system controller, it is also possible to
1
turn off the power individually from the instrument.

When the instrument is connected to the system controller SCL-40/CBM-40, the power
control of the instrument follows the system controller, and the shutdown and startup
functions operate from the system controller throughout the system.

If you turn off the power to the instrument individually with the setting [1], the
connection to the system controller will also be lost. Therefore, the system
startup function does not turn on the instrument. You need to turn the power
on again by pressing and holding the power button on the instrument. The
system shutdown function is still enabled with setting [1]. However, if the power
is turned off by the shutdown function, turning on the power by pressing the
power button of the instrument is disabled, and the system must be started up
from the system controller.

If the [OPERATION MODE] is set to [1] and connected as SPD-20A/SPD-20AV, the

power control function by the system controller is not available. Therefore, you
can always operate the power button of the instrument regardless of the setting
of this function.

"[OPERATION MODE]" P.61

n [SELECT EVENT1]

Set the mode of controlling external equipment via the [EVENT1] output (relay 1).
The screen displays the current setting in the upper right.
Press [Enter] to display the numeric
keypad, then input the values and press
[Enter].

Set Value Function

0 Output terminal1 is controlled with the [EVENT1] set value.
1 Input terminal [EVENT1] is used as a start signal of a time program.
2 Turns ON output terminal 1 when scan is started.

n [SELECT EVENT2]

Set the mode of controlling external equipment via the [EVENT2] output (relay 2).
The screen displays the current setting in the upper right.

46 SPD-40 SPD-40V
 3.6 Auxiliary Functions Screen (FUNCTION)

Press [Enter] to display the numeric

keypad, then input the values and press
[Enter].

Set Value Function

0 Output terminal2 is controlled with the [EVENT2] set value.
1 Output terminal2 [EVENT2] is used as an error output signal.

n Setting the cell temperature control ready check function [CELL READY RNG]

This function allows setting the cell

temperature control ready check function.
The screen displays thecurrent setting (°C)
in the upper right. 3
Press [Enter] to display the numeric
keypad, then input the values and press [Enter].

n Setting the range where the cell temperature control is considered ready [CELL READY]

This function allows setting the range where the cell temperature control is considered
ready.
The screen displays the current setting in
the upper right.
Press [Enter] to display the numeric
keypad, then input the values and press
[Enter].

n Changing the function of the direct key 3 [DIRECT KEY3 SET]

The user can change the function assigned

to the direct key 3 between [SCAN] and
[MARK].
The screen displays the current setting in
the upper right.
Press [Enter] to display the numeric keypad, then input the values and press [Enter].
If the set value is 0, the function is [SCAN]. If it is 1, the function is [MARK]. See the
following section for details of [SCAN] and [MARK].
"2.5.2 Operation Area" P.16

3.6.6 [UTILITY] Setting Group

This is the group for setting parameters such as screen display and buzzer that do not
affect analysis results.

SPD-40 SPD-40V 47
 3 Operation

n [KEY CLOSE]

Press [Enter] to prohibit the keypad entry.

From this point, keypad input is disabled.

To release this function, press [Enter]

while pressing [CE].

n Adjusting the brightness of the display panel [BRIGHTNESS]

This function allows adjusting the contrast

of the display panel in four levels.
Press [Enter] to display the numeric
keypad, then input the values and press
[Enter].
Set Value Brightness Level
1 25%
2 50%
3 75%
4 100%

n Setting the buzzer sound [BEEP MODE]

Sets the buzzer sound.

Press [Enter] to display the numeric
keypad, then input the values and press
[Enter].
Set Value Description
The key input sound, as well as the alarm sound to go off when an error
0
occurs, are activated. (Default setting)
1 Only the alarm sound that goes off when an error occurs is activated.
2 All the buzzer sounds are deactivated.

n [DISP OFF TIME]

Sets the time elapsed before the operation

panel automatically turns to the sleep
mode.
Press [Enter] to display the numeric
keypad, then input the values and press [Enter].

48 SPD-40 SPD-40V
 3.6 Auxiliary Functions Screen (FUNCTION)

The setting range is from 0 to 10 minutes, in which the step can be set by minute. If 0
minutes is input, the operation panel remains ON.

Setting "0" minutes causes the display screen to be always on. If the display
screen is always on, some dots frequently lighting will deteriorate in brightness
in a shorter time.

n [DIRECT KEY]

Sets whether to display the confirmation

screen when one of the direct keys
([ZERO], [RUN], and [SCAN]) is pressed.
Press [Enter] to display the numeric
keypad, then input the values and press [Enter]. 3
When the set value is 0, the following
confirmation screen is displayed for about
three seconds when a direct key is pressed.
Press [Enter] to execute. If the set value is
1, the confirmation screen is not displayed.

3.6.7 [EDIT] Setting Group

Use this group to create a time program.

"3.11 Creating Time Program" P.92

SPD-40 SPD-40V 49
 3 Operation

3.7 VP Functions
VP functions support the validation of the instrument by check functions or displaying
the instrument information.
There are four groups for VP functions: Product Information, Maintenance Information,
Validation Support, and Calibration Support.

3.7.1 List of VP Functions

The VP functions are listed in the tables below.

Operation in the table head shows the types of operation described below.
• Display: Check the monitor.

• [Enter]: Press [Enter] to activate the function.

• Numeric keypad: After pressing [Enter] to display Numeric keypad, press [0] - [9] to enter
a value and press [Enter] to accept the value.

n Product Information [PRODUCT INFO] Group

Command Operation Function Page

SERIAL NUMBER Display Displays the instrument serial number P.52
Displays the instrument name and
Version Display P.53
version number

n Maintenance [MAINTENANCE] Information Group

Command Operation Function Page

Displays the instrument's total
TOTAL OP TIME Display P.54
cumulative operating time
Displays D2 (deuterium) lamp operating
D2 LAMP USED TIME Display P.54
time and replacement alert time
W LAMP USED Displays W (tungsten) lamp operating
Display P.54
TIME*1* time and replacement alert time
PART REPLACEMENT Display To show part replacement information. P.55
MAINTENANCE LOG Display Displays maintenance log P.55
OPERATION LOG Display Displays operation log P.55
ERROR LOG Display Displays error log P.55

*1 SPD-40V only

n Validation [VALIDAION] Support Group

Command Operation Function Page

Display・Numeric
DATE Displays/sets the date P.57
keypad

50 SPD-40 SPD-40V
 3.7 VP Functions

Command Operation Function Page

Display・Numeric
TIME Displays/sets the time P.57
keypad
LEAK SENSOR CHECK Display Runs check on leak sensor P.57
Runs auto checks on memory,
AUTO CHECK [Enter] P.58
wavelength accuracy and light intensity
Checks wavelength accuracy using
CAFFEINE CHECK [Enter] P.59
caffeine.

n Calibration [CALIBRATION] Support Group

To display the VP function of the calibration support group, enter a password

and then press [Enter].
The default password is "00000". 3
Command Operation Function Page
OPERATION MODE Numeric keypad To change the mode of the instrument. P.61
DATE FORMAT Numeric keypad To change the date format. P.61
To initialize the parameters of the
INITIALIZE PARAM [Enter] key P.61
instrument.
For setting the parameter lock with
PARAMETER LOCK Numeric keypad P.61
password
For setting whether to use the part
PARTS MGMT TOOL Numeric keypad P.62
management function or not.
PASSWORD SETTING Numeric keypad For changing password P.62
To display the parameters of CBM.
CBM PARAMETER - This item is displayed only when the P.63
instrument is linked to CBM.
For setting the operation level of leak
LEAK THOLD Numeric keypad P.63
sensor
WAVE CALIB [Enter] key To calibrate the wavelength accuracy. P.63
For inputting D2 lamp replacement alert
D2 TIME Numeric keypad P.63
time
For inputting alert energy value for D2
D2 ENERGY Numeric keypad P.64
lamp replacement

*1 For inputting W lamp replacement alert

W TIME Numeric keypad P.64
time

*１ For inputting alert energy value for W

W ENERGY Numeric keypad P.64
lamp replacement
ABS CALIB [Enter] key For calibration of absorbance accuracy P.65
To specify whether to check the
INITIAL 241nmCHK Numeric keypad wavelength accuracy at 241 nm during P.65
start-up of the instrument.

SPD-40 SPD-40V 51
 3 Operation

Command Operation Function Page

Normally, the absorbance baseline is set
to connect smoothly even if the
wavelength is changed, but it is
Z WAVE Numeric keypad P.65
necessary to change the setting when
performing the wavelength accuracy test
with manual wavelength change.

*1 SPD-40V only

3.7.2 Displaying the VP Functions

1 Press [CE].
The initial screen is displayed.

2 Press [→] and move the cursor to VP

before pressing [→].
The screen displays the VP function groups.

3 Select a group of the VP function by pressing [↑] or [↓] and then press [→].
The screen displays the functions of the group.
Press [↑] and [↓] to select a function.

Press [CE] to return to the initial screen.

To display the VP function of the calibration support group, enter a password

and then press [Enter].
The default password is "00000".

3.7.3 Product Information Group

This group provides information about the instrument.

n [SERIAL NUMBER]

Shows the serial number of this

instrument.

52 SPD-40 SPD-40V
 3.7 VP Functions

n [Version]

Shows the name of software (same as the

model name) and version.

SPD-40 SPD-40V 53
 3 Operation

3.7.4 Maintenance Information Group

This group provides the maintenance-related information.

n [TOTAL OP TIME]

Shows the total operating time of this

instrument.
(The example on the right shows an
operating time of 123 hours.)

n [D2 LAMP USED TIME]

Displays the deuterium lamp cumulative

operating time and replacement alert time.
(The example on the right shows an
operating time of 12 hours)

As for D2 lamp, it is impossible to reset the used time because the lamp itself
memorizes the used time.
The replacement alert time of the D2 lamp is 2,000 hours.

"4.7 Replacing the Lamp" P.117

n [W LAMP USED TIME]

This item is displayed only in the SPD-40V.

Displays the tungsten lamp cumulative

operating time and replacement alert time.
(The example on the right shows an
operating time of 3 hours)
When the tungsten lamp is replaced, Press [Enter] to display the numeric keypad, then
press [0] and [Enter] to reset the operating time to zero (the replacement will be entered
in the maintenance log).

The replacement alert time of the W lamp is 2,000 hours.

"4.7 Replacing the Lamp" P.117

54 SPD-40 SPD-40V
 3.7 VP Functions

n [PART REPLACEMENT]

When not using the parts management

tool, if you replace a general part, enter
the part number.
The part No. is recorded in the
maintenance log.

When PARTS MGMT TOOL is used, the number of uses of consumables cannot
be reset on the operation panel. For the operating procedure, refer to the
instruction manual of PARTS MGMT TOOL.

3
n [MAINTENANCE LOG]

Pressing [→] in the maintenance log screen displays ten records of previously replaced
parts with the dates of their replacement.
Pressing [↑] or [↓] repeatedly displays replaced parts in sequence.
Press [↑] or [↓] repeatedly to show Log1 to Log10 in sequence.
If less than 10 logs are recorded, the
screen displays [No more Logs].
Press [CE] to return to the title screen.

n [OPERATION LOG]

Pressing [→] in the operation log screen

shows the log, which contains the most
recent password changes, parameter
initializations, etc. (up to 10).
Press [Enter] repeatedly to show Log1 to Log10 in sequence.
The figure shows an example that the
parameter lock was canceled on May 12,
2003.
Press [CE] to return to the title screen.

n [ERROR LOG]

Pressing [→] in the error log screen shows

the log, which contains the most recent
errors (up to 10) with their dates.
Press [Enter] repeatedly to show Log1 to
Log10 in sequence, and return to the title screen.
The figure shows an example that liquid leakage was detected on May 12, 2003.

SPD-40 SPD-40V 55
 3 Operation

If less than 10 logs are recorded, the

screen displays [No more Logs].
Press [CE] to return to the title screen.

56 SPD-40 SPD-40V
 3.7 VP Functions

3.7.5 Validation Support Group

This group checks whether the instrument is running correctly.

n [DATE]

Shows/enters the date.

The value returns to the initial value

[00-00-00] after turning the power
OFF.
When the instrument is controlled by
a system controller, the date is
transmitted when connecting.

3
Example: Inputting "March 1, 2019"

1 Press [Enter] to display the numeric keypad.

2 Enter year, month and day in 2 digits and press [Enter]. Enter the last two digits
for the year.

n [TIME]

Shows/enters the time.

The value returns to the initial value

[Link] after turning the power
OFF.
When the instrument is controlled by
a system controller, the time is
transmitted when connecting.

Example: Inputting [Link] a.m.

1 Press [Enter] to display the numeric keypad.

2 Enter hour, minute and second in 2 digits and press [Enter].
The display uses the 24-hour clock.

n [LEAK SENSOR CHECK]

Carries on the operation test for leak

sensor.

SPD-40 SPD-40V 57
 3 Operation

1 Use a syringe filled with water to wet

the thermosensor at the bottom of the
leak sensor.

The leak sensor is installed on

the lower right side inside the
front panel.

2 Wait about 10 seconds. Then press

[Enter].

• If the sensor detects a leakage, [Sensor

Good] will be shown on the screen.

• If not, [Sensor No Good] will be shown.

When [Sensor No Good] is

displayed, refer to "4.10 Leak
Sensor Calibration" P.136

n [AUTO CHECK]

1 Press [Enter].
The following checks will be executed consecutively:
• Memory check1

• Wavelength accuracy1

• Result of wavelength accuracy at last wavelength calibration1

• Result of last leak sensor test1

• Light intensity [D2 ENERGY] [W ENERGY]2

• Lamp operating time [D2 TIME] [W TIME]2

When all of checks pass, [CHECK GOOD] is displayed.
1: When the check fail, [CHECK NO GOOD] is displayed.
2: When the check fail, [CHECK WARNING] is displayed.

2 Press [CE] to erase the resulting display.

58 SPD-40 SPD-40V
 3.7 VP Functions

n Check wavelength accuracy using caffeine [CAFFEINE CHECK]

Check wavelength accuracy at 205 nm,

245 nm, and 273 nm by using caffeine
solutions.

1 The displays on the right appear

alternately.
After enclosing caffeine, press the
[Enter] key.

3
2 The display on the right appears
for about 30 seconds.

3 Check wavelength is measured.

4 The displays on the right appear

alternately.
Then, measure the mobile phase.
After enclosing the mobile phase,
press the [Enter] key.

5 The display on the right appears

for about 30 seconds.

6 The wavelength of the mobile

phase is measured.

SPD-40 SPD-40V 59
 3 Operation

7 If the wavelength accuracy is

confirmed as a result of checking
the wavelength, the display on
the right appears.

If the result is poor,

the display on the
right appears.
If [CHECK NO GOOD]
is displayed, see "If
an alarm sounds and
a [WAVE CHECK NO
GOOD] message is
displayed on the
screen: " P.19

8 The deviation of wavelength from

205 nm, 245 nm, and 273 nm are
displayed.

3.7.6 Calibration Support Group

This group calibrates the instrument.

To display the calibration support group, enter a password.

The instrument is adjusted before leaving the factory. Do not change values
unnecessarily.

n [Input PASSWORD]

Point the cursor to [CALIBRATION]

(calibration support information group)
The PARAMETER setting group, and press
[Enter].
[Input PASSWORD] is displayed.
Input five numbers and press [Enter].

Password should be registered by a system manager.

Be sure to input five numbers. The default password is [00000].

When the password matches, the

[OPERATION MODE] screen below appears
on the screen.

If the password is not input correctly, succeeding steps cannot be accessed.

60 SPD-40 SPD-40V
 3.7 VP Functions

n [OPERATION MODE]

Change the instrument mode to 20A

compatible mode.

[OPERATION MODE] is used

When the instrument is
connected to
CBM-20A/CBM20-Alite.

"3.12 Connection with the System Controller" P.98

Press [Enter] to display the numeric keypad, then input the values and press [Enter].
• The set value "0" is identified and operated as SPD-40/SPD-40V.
• The set value "1" is identified and operated as SPD-20A/SPD-20AV. 3
If 1 is set to connect with CBM-20A or CBM-20Alite, the instrument is recognized
as SPD-20A/SPD-20AV for operation. Therefore, the following restrictions are
applied.
• The set waveform range and the range of spectrum measurement waveform
will be between 190 nm and 900 nm.

n Changing the displayed order of year, month and day [DATE FORMAT]

This function changes the date format.

The current setting is displayed in the
upper left.
The setting 0 corresponds to the format
"year/month/day", the setting 1 "day/month/year", and the setting 2 "month/day/year".
Press [Enter] to display the numeric keypad.
Input the desired values and press [Enter].

n [INITIALIZE PARAM]

Initialize the parameters and the time

programs.
Press [Enter].
Initializes [LAMBDA] (wavelength) and
other parameters, and deletes the time program. (This operation is recorded in the
operation log.)

n [PARAMETER LOCK]

In order to prevent from changing

parameters except the system
administrator, they should be changed to
[On] from [Off]. When [On] is set, the
password screen is displayed.

SPD-40 SPD-40V 61
 3 Operation

When this setting is changed or inputting the password releases the lock, the date is
recorded in operation log.
"[OPERATION LOG]" P.55

n Setting the usage of the parts management tool[PARTS MGMT TOOL]

Sets the usage of the parts management

tool.
For details on the parts management tool
(PARTS MGMT TOOL), refer to the PARTS
MGMT TOOL instruction manual.

Set Value Description

0 Do not use PARTS MGMT TOOL.
1 Use PARTS MGMT TOOL.

When the setting is changed, the change is recorded in the operation log.
"[OPERATION LOG]" P.55

n [PASSWORD SETTING]

Enables to change the password.

1 Press [Enter].
The password must consist of five digits.

2 Input a new password and press [Enter]. Input a new password and press.
The password must consist of five digits.

3 To confirm, input the same password

again.

When the new password is registered,

[PASSWORD CHANGED] is displayed.

If not, [PASSWORD WRONG] is

displayed. In this case, the
password newly entered is not
registered.

62 SPD-40 SPD-40V
 3.7 VP Functions

4 Press [Enter] to return to the title screen.

Record the new password in a secure place. Do not share the password.

n [CBM PARAMETER]

Shows/sets parameters of
CBM-40/SCL-40/CBM-20A which controls the
instrument.
Press [Enter] to display and set each items
in "3.7.7 Showing/setting CBM parameter (Calibration Support Group)" P.67
"3.7.7 Showing/setting CBM parameter (Calibration Support Group)" P.67
3
n [LEAK THOLD]

Input the level (threshold value) at which

the leak sensor is actuated. Use numeric
keys to input the level, and press [Enter].
The setting range is [0-255].
The [ActLv] in the the second row shows the leak sensor's current (actual) value. If this
value exceeds the value set for [LEAK THR], the sensor detects a leak. Factory default
values are input before shipping.
"4.10 Leak Sensor Calibration" P.136

n [WAVE CALIB]

Press [Enter]. The instrument's wavelengths

will be calibrated automatically.
When calibration is complete, the [WAVE
CHECK] (wavelength accuracy check) will
be run automatically, and the result ([GOOD] or [NO GOOD]) displayed.

In about 90 seconds, calibration will complete, and within the succeeding 60 seconds,
wavelength accuracy check will complete.

"4.8 Wavelength Accuracy Calibration" P.130

n [D2 TIME]

Enter the replacement alert time for the

deuterium lamp.
Use numeric keys to input the time, and
press [Enter].

SPD-40 SPD-40V 63
 3 Operation

When the "[AUTO CHECK]" P.58 is run, [WARNING] is displayed if the operating time of
the deuterium lamp has exceeded the value set here.
"[AUTO CHECK]" P.58

The default setting is 2000 hours.

n [D2 ENERGY]

Input the energy alert value for

replacement of the deuterium .
Specifically, set the minimum acceptable
reference energy level of the deuterium
lamp at a wavelength of 220 nm.
Use numeric keys to input the value, and press [Enter].
When the "[AUTO CHECK]" P.58 is run, [WARNING] is displayed if the energy level of
the deuterium lamp at 220 nm is lower than the value set here.
"[AUTO CHECK]"

The default setting is 200 [mV].

n [W TIME]

This function is only available in the SPD-40AV.

Input the replacement alert time for the

tungsten lamp.
Use numeric keys to input the time, and
press [Enter].
When the "[AUTO CHECK]" P.58 is run, [WARNING] is displayed if the operating time of
the tungsten lamp has exceeded the value set here.
"[AUTO CHECK]" P.58

The default setting is 2000 hours.

n [W ENERGY]

This function is only available in the SPD-40V.

Input the energy alert value for

replacement of the tungsten lamp.
Specifically, set the minimum acceptable
reference energy level of the tungsten
lamp at a wavelength of 540 nm.
Use numeric keys to input the value, and press [Enter].

64 SPD-40 SPD-40V
 3.7 VP Functions

When the "[AUTO CHECK]" P.58 is run, [WARNING] is displayed if the energy level of
the tungsten lamp at 540 nm is lower than the value set here.
"[AUTO CHECK]" P.58

The default setting is 250 [mV].

n Calibrate the absorbance accuracy [ABS CALIB]

Using an appropriate reference filter,

calibrate the absorbance accuracy.
"4.9 Absorbance Accuracy
Calibration" P.132

3
n Specifying whether to check the wavelength accuracy at 241 nm during start-up [INITIAL
241nmCHK]

This function allows specifying whether to

check the wavelength accuracy at 241 nm
during start-up. If a mobile phase with
strong absorption near 241 nm is used, the
wavelength accuracy check can not be
performed correctly. In that case, the wavelength check of 241 nm can be skipped by
setting it to "OFF".
The screen displays the current setting in the upper right. The setting "0" does not check
the wavelength accuracy at 241 nm while the setting "1" checks the wavelength accuracy
at 241 nm.
Pressing [Enter] displays the numeric keypad. Input the desired value and press [Enter].

n Change the setting when performing the wavelength accuracy test with manual
wavelength change 《Z WAVE》

Normally, the absorbance baseline is set to connect smoothly even if the wavelength is
changed, but it is necessary to change the setting when performing the wavelength
accuracy test with manual wavelength change.

The current setting is displayed in the upper right. Normally, use "0", and even if the
wavelength is changed, the absorbance baseline will be connected smoothly. When
changed to "1", the baseline fluctuates according to the change in light intensity when
the wavelength is changed.

SPD-40 SPD-40V 65
 3 Operation

Press the [Enter] key to display the numeric keypad. Enter the number you want to set,
and press the [Enter] key again.

After the wavelength accuracy test, be sure to return to the original setting
"0".

66 SPD-40 SPD-40V
 3.7 VP Functions

3.7.7 Showing/setting CBM parameter (Calibration Support Group)

This allows the user to view or set the

parameters of CBM-40/SCL-40/CBM-20A
that controls the instrument.
Press [→] to enter the CBM parameter
setting screen.
Press [↓] or [↑] several times to select the desired item.
Pressing [CE] at an item in the screen returns the display to the screen as shown in the
figure.

When the instrument is not connected to CBM-40/SCL-40/CBM-20A, or is set to

local mode, the CBM parameter screen will not appear, even if [Enter] is pressed.
3
n List of CBM Parameters

SERIAL NUMBER Displays the serial No. of CBM.

Version Displays the program version No. of CBM.
INTERFACE Sets the transmitting protocol to data processing unit.
ETHERNET SPEED Sets the transmitting speed of Ethernet.*1
USE GATEWAY Sets usage of default gateway or DHCP server.*1
IP ADDRESS Sets IP address of CB.*1
SUBNET MASK Sets subnet mask.*1
DEFAULT GATEWAY Sets default gateway.*1, *2

Select the communication destribution when connecting to an

TRS MODE
LC workstation or a Chromatopac.

*1 Available only to show, when not allowed to change on CBM-40/SCL-40/CBM-20A.

*2 Not available when [USE GATEWAY] is set to "Do not use Default Gateway".

Each parameter is activated after the system controller is restarted.

Refer to CBM-40/SCL-40/CBM-20A instruction manual for details of each parameter.

n Showing the serial number [SERIAL NUMBER]

Shows the serial No. of CBM which

controls the instrument.
On the [CBM PARAMETER] screen, press
[↑] or [↓] repeatedly until [SERIAL
NUMBER] is displayed.
The serial number of the system controller is shown on the bottom line.

SPD-40 SPD-40V 67
 3 Operation

n Showing the version number [Version]

Shows the name of software and version

[Link] CBM which controls the instrument.
On the [CBM PARAMETER] screen, press
[↑] or [↓] repeatedly until [Version] is
displayed.
The system controller name is shown on the top line, and the program version number
is shown on the bottom line.

n Setting the transmitting protocol to data processing unit [INTERFACE]

Sets the transmitting protocol from CBM which controls the instrument to data processing
instrument.

1 On the [CBM PARAMETER] screen,

press [↑] or [↓] repeatedly until
[INTERFACE] is displayed.
The currently set value is shown on the top
line.

2 Press [Enter] to display the numeric keypad, then input the values and press
[Enter].
Set Value Transmitting Protocol
0 To connect with optical cable
1*1 To connect with serial transmission (RS-232C)

2*1 To connect with ethernet

*1 CBM-20A only

n Setting the transmitting speed of Ethernet [ETHERNET SPEED]

Sets transmitting speed of CBM ethernet which controls the instrument.

1 On the [CBM PARAMETER] screen,

press [↑] or [↓] repeatedly until
[ETHERNET SPEED] is displayed.
The program version number is shown on
the top line, and the system controller name is shown on the bottom line.

2 Press [Enter] to display the numeric keypad, then input the values and press
[Enter].

Set Value Transmitting speed

0 Auto Detect
1 10 Mbps, Half Duplex
2 10 Mbps, Full Duplex

68 SPD-40 SPD-40V
 3.7 VP Functions

Set Value Transmitting speed

3 100 Mbps, Half Duplex
4 100 Mbps, Full Duplex

n Setting the usage of default gateway or DHCP server [USE GATEWAY]

Set whether or not the default gateway of the CBM that controls this instrument is used.

1 On the [CBM PARAMETER] screen,

press [↑] or [↓] repeatedly until [USE
GATEWAY] is displayed.
The first line shows the current set value.

3
2 Press [Enter] to display the numeric keypad, then input the values and press
[Enter].

Set Value Description

0 The default gateway is not used.
1 The default gateway is used.
2 Automatic acquisition of IP address from DHCP server.

n Setting IP address [IP ADDRESS]

Sets the IP address of the CBM which controls the instrument.

1 On the [CBM PARAMETER] screen,

press [↑] or [↓] repeatedly until [IP
ADDRESS] is displayed.
The current set value is shown on the
bottom line.

2 Press [Enter] to display the numeric keypad, then input the values and press
[Enter].

Consult the network administrator for the value to be set.

n Setting the subnet mask [SUBNET MASK]

Sets subnet mask of CBM which controls the instrument.

1 On the [CBM PARAMETER] screen,

press [↑] or [↓] repeatedly until
[SUBNET MASK] is displayed.
The current set value is shown on the
bottom line.

SPD-40 SPD-40V 69
 3 Operation

2 Press [Enter] to display the numeric keypad, then input the values and press
[Enter].

Consult the network administrator for the value to be set.

n Setting the deault gateway [DEFAULT GATEWAY]

Sets default gateway of CBM which controls the instrument.

1 On the [CBM PARAMETER] screen,

press [↑] or [↓] repeatedly until
[DEFAULT GATEWAY] is displayed.
The current set value is shown on the
bottom line.

2 Press [Enter] to display the numeric keypad, then input the values and press
[Enter].

Consult the network administrator for the value to be set.

n [TRS MODE]

Sets the use of serial communication of CBM that controls this instrument.

1 On the [CBM PARAMETER] screen,

press [↑] or [↓] repeatedly until [TRS
MODE] is displayed.
The currently set value is shown on the top
line.

2 Press [Enter] to display the numeric keypad, then input the values and press
[Enter].

Set Value Description

0 No change in communication setting (default)
1 Not available
2 To connect to CLASS-VP5/6/7
3 To connect to LCsolution
4 to 10 Not used
11 To connect to C-R8A
12 To connect to C-R7A/C-R5A
13 To connect to C-R4A
14 To connect to C-R6A (without extended ROM board)

70 SPD-40 SPD-40V
 3.7 VP Functions

Set Value Description

15 To connect to C-R6A (with extended ROM board)
16 to 19 Not used

SPD-40 SPD-40V 71
 3 Operation

3.8 Measuring in Single Wavelength Mode

• The function is not available when controlled by the system controller.

Configure from the workstation. See " system guide 2.2.1 Editing Methods"
• Refer to the following procedure when using this function in local mode.

3.8.1 Setting the Measurement Range

n When a Chromatopac (Integrator) Is Used As a Recorder:

• The Shimadzu Chromatopac is typically connected to the [INTEGRATOR] connector.

• It is necessary to make approximate range setting on the detector, since the detector's
dynamic range is extremely wide. This is done by setting a value for the detector's
"Setting integrator output range[AUX RANGE]" P.37 parameter.
"Setting integrator output range[AUX RANGE]" P.37

The relation between the [AUX RANGE] settings and the [INTEGRATOR] connector output
AUX RANGE Setting [INTEGRATOR] connector output
1 0.5 AU/V
2 1.0 AU/V
3 2.0 AU/V
4 4.0 AU/V
5 1.25 AU/V
6 2.5 AU/V

Relationship between [AUX RANGE] and [ATTEN] settings and a full scale plot (unit: AU)
The Chromatopac plot full scales produced by the various [AUX RANGE] and [ATTEN]
settings are given in the table below.

AUX RANGE
ATTEN
1 2 3 4 5 6
0 0.0005 0.001 0.002 0.004 0.00125 0.0025
1 0.001 0.002 0.004 0.008 0.0025 0.005
2 0.002 0.004 0.008 0.016 0.005 0.01
3 0.004 0.008 0.016 0.032 0.01 0.02
4 0.008 0.016 0.032 0.064 0.02 0.04
5 0.016 0.032 0.064 0.128 0.04 0.08
6 0.032 0.064 0.128 0.256 0.08 0.16
7 0.064 0.128 0.256 0.512 0.16 0.32
8 0.128 0.256 0.512 1.024 0.32 0.64
9 0.256 0.512 1.024 2.048 0.64 1.28

72 SPD-40 SPD-40V
 3.8 Measuring in Single Wavelength Mode

AUX RANGE
ATTEN
1 2 3 4 5 6
10 0.512 1.024 2.048 4.096 1.28 2.56

SPD-40 SPD-40V 73
 3 Operation

n When a Strip-Chart Recorder Is Used:

• Connect the recorder to the [RECORDER] connector.

• The recorder range should be set at about 120% of the expected maximum peak. This
will produce a chromatogram with maximum peaks of about 80% of the recorder's full
scale.

Proceed as follows to set the recorder range.

Example: To change the range from 0.001AUFS to 0.01 AUFS.

1 Press [CE].
The initial screen is displayed.

2 Press [→] twice.

[PARAMETER] is displayed.

3 Press [→], and then press [↑] or [↓]

several times.
[RANGE] and the current setting appears.

4 Press [Enter] to display the numeric

keypad, then press [0], [・], [0], [1] and
[Enter].

Press [CE] twice, and the initial screen

is displayed.

74 SPD-40 SPD-40V
 3.8 Measuring in Single Wavelength Mode

n When a Chromatopac (Integrator) is connected to the [RECORDER] Connector

• Connect the Chromatopac (integrator) to the [RECORDER] connector.

• Peaks approximately 100 times the range can be recorded. Set the range at about 1/80
of the expected maximum peak absorbance during measurement. Normally, the
recorder range should be set to about 0.005 to 0.04 AUFS, and the full scale plot
adjusted using the [ATTEN] attenuation control of the Chromatopac.

• The relationship in terms of plot full scale between the range and the Chromatopac's
[ATTEN] setting (when the Chromatopac is connected to the [RECORDER] connector) is
as follows:

Plot full scale absorbance = Range ´ 2ATTEN/10

Example: If range = 0.01 AUFS and ATTEN = 2

Plot full scale absorbance = 0.01 AUFS ´ 22/10 = 0.004 AUFS

For simultaneous processing of two channels by a Chromatopac:

In the dual-wavelength measurement mode, chromatograms for each wavelength can be
recorded simultaneously. The wavelength linked to the [INTEGRATOR] connector (the
shorter wavelength end) is regarded as channel 1 and that linked to the [RECORDER]
connector (the longer wavelength end) as channel 2. By using the settings displayed in
the table at right for [RANGE] setting value and [AUX RANGE], the signal to output
voltage ratio is equalized for both channels.
"3.9.3 Setting the Output Mode" P.79

AUX RANGE RANGE

1 (0.5 AU/V) 0.005 AUFS
2 (1 AU/V) 0.01 AUFS
3 (2 AU/V) 0.02 AUFS
4 (4 AU/V) 0.04 AUFS
5 (1.25 AU/V) 0.0125 AUFS
6 (2.5 AU/V) 0.025 AUFS

SPD-40 SPD-40V 75
 3 Operation

3.8.2 Zeroing an Output to a Strip-Chart Recorder

Before beginning analysis, adjust the zero position of the recorder as follows:

1 Press [CE].
The initial screen is displayed.

2 Press [→] twice.

[PARAMETER] is displayed.

3 Press [→] and then press [↑] or [↓]

several times.
[RANGE (AUFS)] (range setting) appears.

4 Press [0], then [Enter].

This sets the recorder output to 0 mV.

5 Using the recorder's pen position

adjusting knob, move the pen to the
desired 0 or baseline level.

6 Reset the detector's measuring range to a range appropriate for the analysis.
"3.8.1 Setting the Measurement Range" P.72

7 Press [0].
The pen will return to a position close to the selected baseline.
To shift the baseline, perform the steps 8 to 10 below.

8 On the initial screen, press [→] twice.

[PARAMETER] is displayed.

9 Press [→], then press [↑] or [↓]

several times.
[BL OFFSET REC] is displayed.

76 SPD-40 SPD-40V
 3.8 Measuring in Single Wavelength Mode

10 Enter the baseline offset value using the numeric keypad, then press [Enter].
Acceptable values are from 2 to 250 in 1mV steps.
"[BL OFFSET REC]" P.38

Pressing [zero] will restore the baseline that was set in this procedure.

3.8.3 Setting [RESPONSE]

This detector uses a digital noise filter to improve the signal-to-noise (S/N) ratio. Noise
decreases as the filter's time constant is raised, and increases as it is lowered.
The response of digital filter on this instrument can be configured as follows using the
parameter [RESPONSE].
• When connected with CBM: 3
Can be set to any of the following three levels: high speed, standard, or low speed.
The correspondence of the set value and response is shown below.

Set Value Responce

1 Fast (Sampling Ratio ´ 1)

2 Standard (Sampling Ratio ´ 2)

3 Slow (Sampling Ratio ´ 4)

• When not connected with CBM or in the SPD-20A compatible mode:

Can be set to any of the following 11 levels: 0 to 10. The following table shows the
time constant of the conventional analog filter corresponding to the change in
responsiveness made by the set value of [RESPONSE].

Set Corresponding time constant of Minimum peak width

Value analog filter at half-height (See NOTE.)
0 No filtering 0.08 sec min.
1 0.05 sec (FAST) 0.2 sec min.
2 0.1 sec 0.4 sec min.
3 0.5 sec (STD) 2.2 sec min.
4 1.0 sec 4.8 sec min.
5 1.5 sec (SLOW) 7.2 sec min.
6 3.0 sec 13 sec min.
7 6.0 sec 26 sec min.
8 8.0 sec 36 sec min.
9 10.0 sec 45 sec min.
10 2.0 sec 9 sec min.

When operating in the dual-wavelength measurement mode, [RESPONSE] values

of 4 or less will not increase time constant; use a value of 5 or greater.

SPD-40 SPD-40V 77
 3 Operation

1 Press [CE].
The initial screen is displayed.

2 Press [→] twice.

[PARAMETER] is displayed.

3 Press [→] and then press [↑] or [↓]

several times.
[RESPONSE] is displayed.

4 Input the [RESPONSE] value using numeric keys.

5 Press [Enter].

6 Press [CE] twice.

The initial screen is displayed.

As [RESPONSE] (time constant) increases, data processor responsiveness and peak

height decrease. The smaller a peak's width at half-height, the greater the
decrease in the peak's height.
It is recommended that [RESPONSE] be set such that, for a given half-height
width, the peak height drops no more than 10 %. The graph below shows the
relationship among response time, peak half-height width and peak height
reduction. Use it to determine appropriate [RESPONSE] values.
Note that [RESPONSE] has no effect on peak area. Peak area does not change
even when a low [RESPONSE] value broadens the peak.

78 SPD-40 SPD-40V
 3.9 Operation in Dual Wavelength Mode

3.9 Operation in Dual Wavelength Mode

• The function is not available when controlled by the system controller.

Configure from the workstation. See " system guide 2.2.1 Editing Methods"
• Refer to the following procedure when using this function in local mode.

3.9.1 Selecting a Measurement Mode

Change the measurement mode from single-wavelength mode to dual-wavelength

measurement mode.
"Setting the mode to dual-wavelength measurement mode [WAVE MODE]" P.35
3
3.9.2 Setting the Measurement Wavelength

Refer to "Setting Wavelength1 [LAMBDA 1]" P.34 and "Setting Wavelength2 [LAMBDA
2]" P.35, set the measurement wavelength.

3.9.3 Setting the Output Mode

The dual-wavelength measurement mode permits either (a) simultaneous recording of

chromatograms for two wavelengths or (b) simultaneous recording of a chromatogram
from Ch1 wavelength and ratio chromatogram of two wavelengths on Ch2. In this mode,
signals are output simultaneously to the [INTEGRATOR] and [RECORDER] connectors. Ch1
absorbance signals are always output to the [INTEGRATOR] connector. The signals output
to the [RECORDER] connector, however, depend on the [REC MODE] function setting:

n Setting the output mode to [RECORDER] connector

1 Press [CE].
The initial screen is displayed.

2 Press [→] twice.

[PARAMETER] is displayed.

3 Press [→].
The wavelength setting screen is displayed.

SPD-40 SPD-40V 79
 3 Operation

4 Press [↑] or [↓] repeatedly until [REC

MODE] (recorder mode setting) is
displayed.

5 Press [Enter] to display the numeric keypad, then input the desired values.

Set Value Output Mode

0 Ch1 absorbance output
1 In the dual-wavelength measurement mode, Ch2 absorbance output
In the dual-wavelength measurement mode, ratio chromatogram signal
2
output to [RECORDER] connector
In the dual-wavelength measurement mode, ratio chromatogram signal
3
output to [INTEGRATOR] connector
4 Temperature adjustment cell's temperature output (100 °C/10 mV)

3.9.4 Setting the Ratio Chromatogram Signal Output Value

The output value of the ratio signal is calculated as follows:

(When Aλ1(t) > Aλ2(t))

(When Aλ1(t) £ Aλ2(t))

* Aλ1(t) and Aλ2(t) represent Ch1 absorbance and Ch2 absorance respectively.

n Sending a Ratio Chromatogram to the Chromatopac:

1 Press [CE].
The initial screen is displayed.

2 Press [→] twice.

[PARAMETER] is displayed.

3 Press [→].
The wavelength setting screen is displayed.

80 SPD-40 SPD-40V
 3.9 Operation in Dual Wavelength Mode

4 Press [↑] or [↓] repeatedly until [REC

MODE] (recorder mode setting) is
displayed.
"[REC MODE]" P.38

5 Press [3].

6 Set the Chromatopac [ATTEN]

parameter to zero.
When a ratio chromatogram signal is being
output, the baseline is automatically 3
shifted to the midpoint of the detector's
recorder output range (i.e. the baseline
will move +5mV at the 10mV recorder
terminal). From this midpoint, the plot
goes up when Ch1 absorbance exceeds Ch2
and down when the reverse is true.

n Setting [RATIO RANGE]

Set the magnification of ratio chromatogram signal R(t) at full scale to the parameter
[RATIO RANGE].

Example: To set [RATIO RANGE] to "10"

1 Press [CE].
The initial screen is displayed.

2 Press [→] twice.

[PARAMETER] is displayed.

3 Press [→].
The wavelength setting screen is displayed.

4 Press [↑] or [↓] repeatedly until

[RATIO RANGE] is displayed.

SPD-40 SPD-40V 81
 3 Operation

5 Press [Enter] to display the numeric

keypad, then press [1], [0] and [Enter].
(The setting range is 2 to 10000.)

If the peak in which R(t) is 5 elutes, half

of full scale, or 25-scale ratio
chromatogram, is output.

n Setting [RT THR] (Ratio Threshold)

The threshold value, which controls whether or not a ratio is output, is set using [RT THR].

1 Press [CE].
The initial screen is displayed.

2 Press [→] twice.

[PARAMETER] is displayed.

3 Press [→].
The wavelength setting screen is displayed.

4 Press [↑] or [↓] repeatedly until

[RATIO THR] is displayed.

82 SPD-40 SPD-40V
 3.9 Operation in Dual Wavelength Mode

5 Press [Enter] to display the numeric keypad, then input the values.
(The setting range is 0.0001 to 1.0 AU.)
A ratio chromatogram is output only when both the Ch1 and Ch2 signals exceed the
value set for [RT THR].
Assuming no baseline drift, [RT THR] should be set at a value equal to 1-5% of the full
scale measuring range (the Range Value). If the baseline drifts, for example in gradient
analysis, execute an auto zero operation in the time program shortly before the peak of
interest elutes, and set [RT THR] to a value larger than the expected drift.

Be sure to actuate the auto zero function at the beginning of each analysis.
Absorbance values used in the calculation of ratios are relative to the auto zero
point.

SPD-40 SPD-40V 83
 3 Operation

n Drift-Related Distortion in a Ratio Chromatogram

Ratio chromatograms are derived from the following equation, where Al1(t) and Al2(t)
represent the change in absorbance, relative to the auto zero point, in wavelengths l1
and l2 respectively.

(When Aλ1(t) > Aλ2(t))

* Aλ1(t) and Aλ2(t) represent Ch1 absorbance and Ch2 absorance respectively.
However, since in actuality Al1(t) and Al2(t) also include baseline drift Dl1 and Dl2,
the equation from which the ratio chromatograms are derived is, more accurately:

When the drift is zero, R for a pure peak

maintains a constant value, and the ratio
chromatogram shows a flat-topped peak.

No drifting

When drift is present in R, the

chromatogram will be distorted as shown
on the right.
For minimum distortion in the ratio
chromatogram:
• set [RT THR] about 10 times larger than
the drift.
• maximum absorbance of peak AMAXl1 Drift of l1 is large.

and AMAXl2 should be at least twice the

[RT THR] value.
• select two wavelengths which are
strongly absorbed by the sample but
weakly absorbed by the mobile phase

Drift of l2 is large

84 SPD-40 SPD-40V
 3.10 Operation in Spectrum Scanning Mode

3.10 Operation in Spectrum Scanning Mode

This instrument has the spectrum scanning function for collecting two sample spectra and
one background spectrum.
It is necessary to make sure liquid in the cell does not move during scanning. Do the
following before scanning spectrum.
• When using SIL or manual injector:
Check the absorbance on the initial screen and stop feeding the liquid when the
specimen reaches the cell.
• When using a syringe:
After stopping feeding of the liquid, perform injection.

3.10.1 Setting Parameters Necessary to the Spectrum Measurement 3

1 Press [CE].
The initial screen is displayed.

2 Press [→] twice.

[PARAMETER] is displayed.

3 Press [→].
The wavelength setting screen is displayed.

4 Press [↑] or [↓] repeatedly until [SCAN FILE] (spectrum scan file) is displayed.
Then press [Enter].

5 [Press [Enter] to display the numeric

keypad, then press [0] and [Enter].

[SCAN FILE] 0 must be used for storing the background spectrum and Files 1 and
2 for storing samples. During processing, File 0 is automatically subtracted from
the scan data in Files 1 and 2. Set the [SCAN FILE] to [0] before filling the cell
with mobile phase.

"[SCAN FILE]" P.41

SPD-40 SPD-40V 85
 3 Operation

6 Set the scan start wavelength.

The wavelength settings range

displayed on the second row
differs by the model according
to the table of "Start/End
Wavelength Setting Range"
P.86.

1 Press [↑] or [↓] repeatedly until [SCAN BEGIN] is displayed.

2 Press [Enter] to display the numeric keypad, then input the values and press [Enter].
"[SCAN BEGIN]" P.41

7 Set the scan end wavelength.

The wavelength settings range

displayed on the second row
differs by the model according
to the table of "Start/End
Wavelength Setting Range"
P.86

1 Press [↑] or [↓] repeatedly until [SCAN END] is displayed.

2 Press [Enter] to display the numeric keypad, then input the values and press [Enter].
"[SCAN END]" P.41

Start/End Wavelength Setting Range

Wavelength Setting Range

Model (Lamp) End wavelength
Start wavelength
Operation panel Labsolutions
(Start wavelength + 1)～ （Start wavelength +
190～369 nm
700 nm 1）～370 nm
SPD-40
(Start wavelength + 1)～ （Start wavelength +
371～699 nm
700 nm 1）～700 nm

86 SPD-40 SPD-40V
 3.10 Operation in Spectrum Scanning Mode

Wavelength Setting Range

Model (Lamp) End wavelength
Start wavelength
Operation panel Labsolutions
（Start wavelength + （Start wavelength +
D2 lamp 190～369 nm
1）～370 nm 1）～370 nm
（Start wavelength + （Start wavelength +
371～699 nm
1）～1000 nm 1）～700 nm
W lamp
（Start wavelength + （Start wavelength +
701～999 nm
1）～1000 nm 1）～1000 nm
SPD-40V
（Start wavelength + （Start wavelength +
190～369 nm
1）～1000 nm 1）～370 nm
D2 and W （Start wavelength + （Start wavelength +
371～699 nm
lamps 1）～1000 nm 1）～700 nm
（Start wavelength + （Start wavelength + 3
701～999 nm
1）～1000 nm 1）～1000 nm

8 Set the scan wavelength step.

1 Press [↑] or [↓] repeatedly until

[SCAN STEP] is displayed.

2 Press [Enter] to display the numeric keypad, then input the values and press [Enter].
"[SCAN STEP]" P.42

9 Press [CE].
The initial screen returns.

SPD-40 SPD-40V 87
 3 Operation

3.10.2 Measurement Procedure

1 Enclose the mobile phase in the flow cell.

Connect the syringe (B) to the inlet piping (D) of the cell as shown below. After
connection, enclose the mobile phase.

Signal Part Name

A Plunger
B Syringe
C Coupling1.6-0.8C
D Cell inlet tubing
E Male nutPEEK
F Male nut 0.8MN PEEK

2 Press the [SCAN] key while the flow

cell is in the mobile phase, and
measure background data.
Scanning will begin.
During scanning, [Scanning...] appears on the initial screen.

Check that 0 is set for the scan file.

3 Enclose the sample in the flow cell.

4 When the baseline is stabilized, display

[SCAN FILE] in the [PARAMETER]
group, and press [1] or [2].
"[SCAN FILE]" P.41

88 SPD-40 SPD-40V
 3.10 Operation in Spectrum Scanning Mode

5 Press [SCAN].
Scanning will begin.

It is not possible to program different scanning conditions ([SCAN BEGIN], [SCAN

END], [SCAN STEP]) for the background ([SACN FILE] = 0) and sample ([SCAN
FILE] = 1/2). This will prevent spectrum data from being acquired accurately.

3.10.3 Output of Spectrum Data

The spectrum data contained in the current SCAN Files may be sent to the recorder
terminals and plotted on a recorder. First, set the plot speed:

3
1 Press [CE].
The initial screen is displayed.

2 Press [→] twice.

[PARAMETER] is displayed.

3 Press [→].
The wavelength setting screen is displayed.

4 Press [↑] or [↓] repeatedly until [PLOT

SPEED] (to set the speed at which
spectrum data is sent) is displayed.
Then press [Enter].

[PLOT SPD] Setting Plot Speed

5 Choose the plot speed. Enter the speed
using numeric keys. 1 1 nm/sec
2 3 nm/sec
3 10 nm/sec

6 Press [↑] or [↓] repeatedly.

[SPC PLOT] is displayed.

7 Press [Enter].
Plotting begins.

SPD-40 SPD-40V 89
 3 Operation

n To Stop Plotting

While [l PLOTTIING] is displayed on the

[SPC PLOT] screen, press [Enter].
The spectrum data is sent only to the
[RECORDER] terminals, and is normalized
so the plotted absorbance in the specified
wavelength range peaks at about 70% of
full scale.
For a Chromatopac, set [ATTEN] at [3] or
[4] for plotting.

Example of output
Output duration T is as follows:

T = (SCAN END – SCAN BGN)/ PLOT SPEED

* 1, 3, or 10 nm/sec

For example, when a 200 nm to 350 nm range is to be plotted at a plot speed of

3 nm/sec (PLOT SPD = 2):

T = (350 - 200) nm / 3 nm / sec = 50 sec

• Files which do not contain scanned data cannot be plotted. If an empty file
is specified, the message [DATA NOT EXIST] is displayed. Similarly, plotting
cannot be performed if there is no data in the background file ([SCAN FILE]
= 0).
• If the scanning range includes 370 nm in the SPD-40/SPD-40V or 700 nm in the
SPD-40V, noise caused by insertion of the second-order diffraction filter will
appear at these wavelengths.
• Spectrum data cannot be output to the [INTEGRATOR] connector.
• Spectrum data is lost when the detector is turned off.
• Changing the scanning conditions ([SCAN BGN], [SCAN END], [SCAN STEP]) from
those that were used when scanning was started can prevent data from being
plotted correctly.

n Peak Intensity Required for Spectrum Scanning

When the absorbance of the mobile phase varies substantially over the scanning
wavelength range, the effect of the instrument's wavelength reproducibility error (0.2 nm

90 SPD-40 SPD-40V
 3.10 Operation in Spectrum Scanning Mode

Max) on the spectrum data can be significant. To minimize this effect, the absorbance
value of the peak of interest should be as high as possible. More specifically, absorbance
at the maximum absorbance wavelength of the peak of interest should be about 100
times the maximum absorbance change of the mobile phase per 0.2 nm increment of
the scanning wavelength range.

Example
In the mobile phase absorbance spectrum shown below, the absorbance change per
wavelength is greatest in the section between 210 nm and 220 nm.

The rate of change per nanometer in this section is (1.0 - 0.5) /(220 - 210) = 0.05 AU/nm.
Therefore, the maximum spectrum error which could be introduced by wavelength
reproducibility error is 0.2 nm ´ 0.05 AU/nm = 0.01AU. To obtain a spectrum in which
this error may be ignored, the absorbance maximum of the peak of interest should be
about 100 times the possible error, or about 1 AU.

SPD-40 SPD-40V 91
 3 Operation

3.11 Creating Time Program

A time program is used to program certain functions of the instrument automatically
during an analysis. time programs are retained in memory when the power is turned off.

• The function is not available when controlled by the system controller.

Configure from the workstation. See " system guide 2.2.1 Editing Methods"
• Refer to the following procedure when using this function in local mode.

3.11.1 Program Commands

Command Description Remark

STOP Stops time program None
Repeats preceding time program steps 0 to 255
LOOP
the specified number of times * 0: Repeats program 256 times

Sets the relays ON (close) / OFF (open)

EVNT 0 to 1
"[EVENT1], [EVENT2]" P.39

Refer to the setting ranges in

l1, l2 Wavelength 1, Wavelength 2 the table on "Setting ranges
for l1 and l2" P.93.

ZERO Executes detector autozero None

MARK Marks recorder output None
0 to 2.56
Sets full scale output range of recorder * A range of 0 short-circuits the
RANG
terminals recorder terminals, preventing
signal output.

Sets detector time response 1 to 3 (Operation mode: 40)

RESP
"3.8.3 Setting [RESPONSE]" P.77 0 to 10 (Operation mode: 20A)

Sets the file number where scanned

SCAN 0 to 2
data is to be stored.
0: Positive polarity
POL Sets polarity of detector output
1: Reversed polarity
0: OFF
1: D2 lamp ON

LAMP Turns D2/W lamp ON/OFF 2: W lamp ON

(SPD-40V only)
3: D2 and W lamps ON
(SPD-40V only)
0: OFF
CELT Sets temperature of the flow cell Room temperature
9 °C - 50 °C
0: Auto
S.V. Controls recycle valve
1: Drain

92 SPD-40 SPD-40V
 3.11 Creating Time Program

Setting ranges for l1 and l2

Wavelength Mode Model (Lamp) Setting Range

SPD-40 190 to 700 nm

Single-wavelength D lamp 190 to 370 nm

measurement mode SPD-40V W lamp 371 to 1000 nm
D2 and W lamps 190 to 1000 nm
190 to 370 nm or
SPD-40
371 to 700 nm
D lamp 190 to 370 nm
Dual-wavelength 371 to 700 nm or
measurement mode W lamp
701 to 1000 nm
SPD-40V
190 to 370 nm,
D2 and W lamps 371 to 700 nm or 3
701 to1000 nm

3.11.2 Setting Time Programming Parameters

Sets parameters in the [EDIT] screen to create a time programe.

1 Press [CE].
The initial screen is displayed.

2 Press [→] twice and then press [↑] or

[↓] repeatedly until [EDIT] is displayed.

3 Press [→] once.

The time program edit mode screen is
displayed.

USED The number of steps already set

LEFT The number of steps left available

The figure shows an example of a time program that has ten steps already created and
22 steps left available.

4 Press [Enter].

SPD-40 SPD-40V 93
 3 Operation

5 Input the time that elapses before the

desired function starts running and
then press [Enter].
Users can input the time between 0.01 and
999.99.

Press [↑] or [↓] repeatedly and then press [Enter] in the desired function screen to set
the function.

"3.11.1 Program Commands" P.92

• Pressing [↑] or [↓] repeatedly displays one function after another.

• Pressing [Enter] when a desired function is displayed sets the function.

• When you selected a function other than [ZERO], [MARK], or [STOP], input the setting
value with the numeric keypad and then press [Enter].

6 Press [CE].
The initial screen is displayed.

3.11.3 Creating a Typical Time Program

The following example shows the steps involved in creating a time program which will
execute a spectral scan at five minutes into the analysis. The scanned spectrum data will
be stored in file number two.

1 Press [CE].
The initial screen is displayed.

2 Press [→] twice. Then, press [↑] or [↓]

repeatedly until [EDIT] is displayed.

3 Press [→] once.

The time program edit mode screen is
displayed.

4 Press [Enter].
The time (minute) setting screen is
displayed.

5 Press [5] and [Enter].

94 SPD-40 SPD-40V
 3.11 Creating Time Program

6 Press [↑] or [↓] repeatedly until

[CELT] is displayed.

7 Press [Enter].

8 Input the temperature for the flow cell

temperature control with the numeric
keypad and then press [Enter].
The figure is an example in which the flow
cell temperature is set to 40 °C.

9 Press [Enter]. 3
The time (minute) setting screen is
displayed.

10 Press [10] and [Enter].

11 Press [↑] or [↓] repeatedly until

[STOP] is displayed.

12 Press [Enter].

13 Press [CE].
Finish creating a program.

• When setting several steps, they are ordered automatically in sequence.

• Always end the program with a [STOP] command, unless the program is to
run indefinitely and be stopped manually.

3.11.4 Repeating the Time Program Steps [LOOP]

[LOOP] function can register the loop

count of time program.

SPD-40 SPD-40V 95
 3 Operation

Set "3" for example. Step 1 and 2 will Setting example of [LOOP]

be repeated three times in 30 minutes TIME FUNC VALUE

interval. 1 15.00 l1 210
Set [LOOP] at the end of the program.
2 20.00 l1 220
3 30.00 LOOP 3

• Value of [LOOP] command can be set to 255. When [0] is set, time program
will be repeated 256 times.
• Steps which follow a [LOOP] command are ignored.

3.11.5 Deleting Steps

Call up the steps and press [del].

Example: Delete step 1 of the program set in "3.11.3 Creating a Typical Time Program".

1 Show the step to be deleted.

To delete a subsequent step, press

[↑] or [↓] repeatedly until the
desired step appears.

2 Press [del].
The displayed step will be deleted. If there is a subsequent step, this step will be
displayed.

3.11.6 Starting a Time Program

There are 2 ways to begin the time program after completing the time program settings:
• Press [run].
• A contact closure signal is received through the external input/output terminal.
"3.13 External Input/Output Terminals" P.99

In either case, the time program starts and the time program lamp on the display is
turned on.

If any parameters are changed while the time program is running, the new
parameter take effect during the time program run. After the time program
ends, the parameters changed while the time program was running are set to
the previous values entered before the time program start.

96 SPD-40 SPD-40V
 3.11 Creating Time Program

3.11.7 Stopping a Time Program

There are 3 ways to stop the time program:

• Press [run] to force a program in progress to stop.
• A contact closure signal is received through the external input/output terminal.
"3.13 External Input/Output Terminals" P.99

• Use the [STOP] command to force a program in progress to stop.

"3.11.3 Creating a Typical Time Program" P.94

SPD-40 SPD-40V 97
 3 Operation

3.12 Connection with the System Controller

3.12.1 Preparation

To control the instrument from the system controller, set the parameters as follows:

Setting
Set Value Remark
Parameter
LOCAL MODE 0: Remote "[LOCAL]" P.45

LINK ADDRESS Link address "[LINK ADDRESS]" P.45

0: 40*1
OPERATION MODE "[OPERATION MODE]" P.61
1: 20A*2

*1 Set "0" to connect with SCL-40/CBM-40/CBM-40lite.

*2 Set "1" to connect with CBM-20/CBM-20Alite.

If 1 is set to connect with CBM-20A or CBM-20Alite, the instrument is recognized

as SPD-20A/SPD-20AV for operation.
Therefore, the following restrictions are applied.
The set waveform range and the range of spectrum measurement waveform will
be between 190 nm and 900 nm.

• "Setting Wavelength1 [LAMBDA 1]" P.34

• "Setting Wavelength2 [LAMBDA 2]" P.35

3.12.2 Basic Parameters

The following settings and operations can be performed from the system controller. For
more details, see the system controller instruction manual.
• Setting wavelength
• Switching between single and dual-wavelength measurement modes
• Turning lamp on/off
• Switching between different lamps (D2, W, D2 and W simultaneous lighting) (SPD-40AV
only)
• Setting output range for integrator
• Setting response
• Setting parameters for solvent recycle valve
• Activating / deactivating cell temperature adjustment and setting temperature
• Creating a time program
System Guide "6.3.2 Optical Cable Connection"

98 SPD-40 SPD-40V
 3.13 External Input/Output Terminals

3.13 External Input/Output Terminals

The external input/output terminals are connected to a event output device or another
external device with a
provided event cable.

Signals Description Remark

EVENT1 (Output) [Event1/2] output terminals. To connect to Contact rating: 30VDC/1A
relay and be turned ON/OFF according to a
EVENT2 (Output) time program or the [EVENT] value of
auxiliary function. These signals are
implemented by shorting
Initiates a time program on this instrument
the appropriate wire pair
by external contact signal.
between the input
START (Input) If a start signal is received while a time
command terminal and
program is running, the program is initiated
the common terminal.
again starting at 0 minutes.
Duration of shorting (tc)
Stops a time program on this instrument by should be as follows.
STOP (Input)
external contact signal. 0.5 sec < tc < 10 sec.
COMMON The common input terminal.

SPD-40 SPD-40V 99
 3 Operation

n Connection of External Input/Output Terminals

Necessary parts

Part Name Part Type Part No.

Event cable Accessory 228-28253-91

1 While holding down a button of an external input/output terminal with a thin

flathead screwdriver, insert the cable.
To pull out the cable, pull it out while holding down the button of the terminal.

2 When connecting two or more circuits to the terminals, use the wires as shown
below.
The instrument provides one event cable. When more than 2 cables are required, use
the following cables.
Before using the cable, strip the jacket (1) at the end approximately 10 mm.

• Cable with single wire: f 0.4 tof 1.2 (AWG26 to 16)

• Cable with stranded wire: 0.3 mm2 to 1.25 mm2 (AWG22 to 16), diameter of
single wire thicker than f 0.18.
• For a stranded cable, twist the ends well.
The cable with stranded wire is suitable to prevent disconnection.

100 SPD-40 SPD-40V

4 Maintenance
4.1 Periodic Inspection and Maintenance
It is necessary to perform periodic inspections of this instrument to ensure its safe use.
It is possible to have these periodic inspections performed by Shimadzu service
representatives on a contractual basis.
For information regarding the maintenance inspection contract, contact your Shimadzu
representative.

Turn off the main power switch on the back of the instrument and unplug
the power cable prior to performing inspections and maintenance when
instructed.
Otherwise, fire, electric shock or malfunction may occur.

Never remove the main cover.

Otherwise, injury or malfunction may occur. Contact your Shimadzu
representative to remove the main cover.

When replacing parts, use only the parts listed in "1.2 Component Parts" and
"5.2 Consumable Parts/Maintenance Parts/Replacement Parts".
If any other parts are used, injury or malfunction may occur.

4.1.1 Prior to Inspection and Maintenance

• Replace the mobile phase in the flow lines with water.

• Wipe away any dirt from the front panel and the main cover.
• Wipe away any dirt from the keypad with tissue paper or a soft cloth moistened with
water.
System Guide "5.3 Troubleshooting and Corrective Action"

SPD-40 SPD-40V 101

 4 Maintenance

4.1.2 List of Periodic Inspection and Maintenance

The replacement and maintenance periods listed in this table are presented only as
guidelines. These are not guarantee periods. These will vary depending on usage
conditions.

Inspection/Maintenance 1 2 3
No. Remark Ref.
Item year year year
Replace at about 2000 hours of total P.
1 D2 lamp replacement P P P
illumination time. 119
W lamp replacement Replace at about 2000 hours of total P.
2 - - P
(SPD-40V only) illumination time. 124
Dismantling, cleaning Replace the flow cell if it is unclean
P.
3 & inspection of flow - P - and light intensity is low even after
108
cell cleaning it.
Replace the gaskets after
Flow cell gasket
4 - P - disassembling and cleaning the flow
replacement
cell. P.
Replace the lenses when some dirt 113
Flow cell lens
5 - P - remains after disassembling and
replacement
cleaning the flow cell.

4.1.3 Post-Inspection/Maintenance Leakage Check

After inspection and maintenance, check any leakage during pumping.

System Guide "5.3 Troubleshooting and Corrective Action"
System Guide "[Link] Does Not Stabilize"
System Guide "5.25 Noise Appears in Baseline"

102 SPD-40 SPD-40V

 4.2 Removing/Installing the Flow Cell

4.2 Removing/Installing the Flow Cell

Be sure to turn off the power of the equipment when plugging or unplugging
the flow cell cable connector.

4.2.1 Removing the Flow Cell

1 Open the front cover.

2 Remove the coupling from the coupling holder (B).

3 Disconnect the connector (A).

Turn off the equipment before unplugging the connector. 4

4 Loosen the 2 cell mounting screws (C) and remove the cell.

SPD-40 SPD-40V 103

 4 Maintenance

4.2.2 Installing the Flow Cell

1 Turn up the connector side and then install the flow cell to the instrument.

2 While holding the flow cell fitted to the installation surface, tighten the two cell
mounting screws (C) alternately.

3 Insert the connector (B) into the instrument. With the coupling holder (A), fix
the coupling to the instrument.

Make sure that the equipment is turned off before plugging the connector.

4 Connect the inlet tubing of the flow cell directly to the column.
Via the coupling, connect the outlet tubing of the flow cell to the drain tubing.

When performing the above operation, do not let any air enter the flow lines.

5 Close the front cover.

104 SPD-40 SPD-40V

 4.3 Flow Cell Inspection/Cleaning

4.3 Flow Cell Inspection/Cleaning

If the baseline shows a large noise or large drift, check the flow cell. If the check finds
some bubbles or dirt, try the simple cleaning.
After cleaning the flow cell with the syringe according to the following procedure, if the
light intensity is not improved, replace the column with the coupling and pump a rinsing
solution (2-propanol) into the flow cell assembly for 2 hours.

Pump a solution from the inlet tubing into the flow cell.
If pumped from the outlet tubing to the inlet tubing, the flow cell may get
damaged and become unusable.

Necessary Parts

Part Name Type Part No.

Syringe, 1ML Standard accessory 228-57028 4
Syringe needle Standard accessory 228-57027
Coupling 1.6-0.8C *1 Standard accessory 228-40998-10
Male nut 0.8MN-PEEK Standard accessory 228-46363
2-propanol ― ―

*1 When using Inert cell, UHPLC Inert cell or Low Dispersion Inert cell, use the supplied
coupling 1.6-0.8C PEEK.

SPD-40 SPD-40V 105

 4 Maintenance

Sign Name Sign Name

A Waste container F Male nut 0.8MN-PEEK
B Cell inlet tubing G Syringe needle
C Cell outlet tubing H Syringe
D Male nut ETFE I Plunger
E Coupling 1.6-0.8C

1 Remove the flow cell.

"4.2.1 Removing the Flow Cell" P.103

2 Set the flow rate to 1 mL/min and then start pumping.

3 While the mobile phase is flowing, look at the inside of the flow cell in the
direction of the arrow to check that no bubble or dirt is in the cell.

If some bubbles or dirt is in the cell, proceed to the simple cleaning.

No simple cleaning is needed if no bubble or dirt is in the cell.

106 SPD-40 SPD-40V

 4.3 Flow Cell Inspection/Cleaning

4 Insert the syringe needle (G) on the tip of the syringe (H) and turn it clockwise
to fix it in place.

5 Remove the tubing on the cell outlet tubing (with a coupling) end.

6 Connect the syringe (H) to the cell outlet tubing (C). (Attach the male nut
0.8MN-PEEK (F) to the syringe needle and connect the coupling 1.6-0.8C (E).)

When using Inert cell, UHPLC Inert cell or Low Dispersion Inert cell, connect the
coupling to the cell inlet tubing.

7 Gently push in the syringe's plunger (I).

The liquid will be injected into the cell interior and clean the cell.

If an aqueous solvent (especially a solvent containing buffer salts) is used as

the mobile phase, purge the solvent in the cell with water before flushing
with 2-propanol.
If an organic solvent is mixed with salts, crystals will form and clog the tubing.

8 Flush the flow cell with distilled water or deionized water using a syringe to
purge the rinsing solution (2-propanol).

9 Perform step 3 to check for bubbles and dirt.

SPD-40 SPD-40V 107

 4 Maintenance

4.4 Disassembling and Cleaning the Flow Cell

If dirt and air bubbles cannot be removed by performing a simple cleaning, disassemble
and clean the flow cell. Also, disassembling and cleaning the flow cell is recommended
every 2 years.

Necessary tools
• Flathead screwdriver (Part No.: 086-10504-18)
• Plastic tweezers (with no dirt on the end)
• Air blower (for the lens) (Part No.: 086-78801-02)

If plastic tweezers are not available, use some kind of alternative item such as
a toothpick for removing. In this case, use something soft so that it does not
scratch the lens or cell window.

Necessary parts

Part Name Part Type Part No. Remarks

Be sure to replace when
Maintenanc 228-35097-95 (2
Gasket disassembling and cleaning the
e part pcs)
flow cell.
2-Propanol - - -
(Mobile Phase Solution
- - -
Used for Analysis)

If use of the gaskets is continued without replacing, fluid may begin to leak.

108 SPD-40 SPD-40V

 4.4 Disassembling and Cleaning the Flow Cell

1 Remove the flow cell.

"4.2.1 Removing the Flow Cell" P.103

2 Remove the lenses and cell gaskets.

1 Loosen the male nut (A) on the column side, and remove the flow cell inlet tubing
(B).
Remove the flow cell outlet tubing (C) in the same way.

2 Remove the left and right setscrews (D) using a flathead screwdriver.

SPD-40 SPD-40V 109

 4 Maintenance

3 Remove the lens (E) and gasket (F) using plastic tweezers.

3 Put the removed lenses in 2-propanol and clean them with an ultrasonic bath
for 5 minutes.

4 Attach the cleaned lenses and two new cell gaskets to the cell.

1 Use a piece of wiping paper soaked in 2-propanol to remove dirt inside the cell
housing (G).

110 SPD-40 SPD-40V

 4.4 Disassembling and Cleaning the Flow Cell

2 If dust is attached to the lenses or gaskets, remove it using an airblower (for the
lens) while securing the lenses or gaskets with plastic tweezers so as not to be
blown away by the air.

If an air blower (for the lens) is not available, use a piece of wiping paper
soaked in 2-propanol to remove the dust.

3 Attach the new gaskets and lenses in that order using plastic tweezers, and attach
the setscrews using a flathead screwdriver.

4 Connect the syringe adapter to the flow cell outlet tubing. (Attach the male nut
0.8MN-PEEK (I) to the syringe needle(J) and connect the coupling 1.6-0.8C 4
(H).)Connect the flow cell inlet tubing to the tubing to the drain.

When using Inert cell, UHPLC Inert cell or Low Dispersion Inert cell,
use the supplied coupling 1.6-0.8C PEEK. In this case, connect the
coupling to the cell inlet tubing.

5 Attach the syringe (K) with mobile phase solution for analysis to the syringe
needle, and slowly push the plunger to check for a fluid leak.

If there is a fluid leak, rinse the lenses again, and then tighten the
setscrews again so that they are not loose.

6 Loosen the male nut (A), and remove the syringe needle.

SPD-40 SPD-40V 111

 4 Maintenance

5 Attach the flow cell.

"4.2.2 Installing the Flow Cell" P.104

112 SPD-40 SPD-40V

 4.5 Replacing a Flow Cell Part

4.5 Replacing a Flow Cell Part

It is necessary to replace the lens or cell window if dirt on the lens or cell window cannot
be removed after disassembling and cleaning the flow cell. The gaskets should be
replaced at the same time. Also, it is recommended to replace the lenses or cell window
every 2 years.

Necessary tools
• Flathead screwdriver (Part No.: 086-10504-18)
• Plastic tweezers (with no dirt on the end)
• Air blower (for the lens) (Part No.: 086-78801-02)

If plastic tweezers are not available, use some kind of alternative item such as a toothpick
for removing. In this case, use something soft so that it does not scratch the lens or cell
window.

Necessary parts

Part Name Part Type Part No. Remarks 4

Be sure to replace when
Maintenance
Gasket 228-35097-95 (2 pcs) disassembling and cleaning the
part
flow cell.
Maintenance 228-73281-41 (2 pcs)
Lens -
part 228-14572 (1 pc)
2-propanol - - -

If use of the gaskets is continued without replacing, fluid may begin to leak.

1 Remove the flow cell.

"4.2.1 Removing the Flow Cell" P.103

SPD-40 SPD-40V 113

 4 Maintenance

2 Remove the lenses and cell gaskets.

1 Loosen the male nut (A) on the column side, and remove the flow cell inlet tubing
(B). Remove the flow cell outlet tubing (C) in the same way.

2 Remove the left and right setscrews (D) using a flathead screwdriver.

3 Remove the lens (E) and gasket (F) using plastic tweezers.

114 SPD-40 SPD-40V

 4.5 Replacing a Flow Cell Part

3 Attach the new lenses and 2 new cell gaskets.

1 Use a piece of wiping paper soaked in 2-propanol to remove dirt inside the cell
housing (G).

2 If dust is attached to the lenses, cell window or new gaskets, remove it using an
air blower (for the lens) while securing the lenses or new gaskets with plastic
tweezers so as not to be blown away by the air.

If an air blower (for the lens) is not available, use a piece of wiping paper
soaked in 2-propanol to remove the dust.

3 Attach the new gaskets and lenses in that order using plastic tweezers, and attach
the setscrews using a flathead screwdriver.

SPD-40 SPD-40V 115

 4 Maintenance

4 Connect the syringe adapter to the flow cell inlet tubing (B). (Attach the male nut
0.8MN-PEEK (I) to the syringe needle(J) and connect the coupling 1.6-0.8C
(H).)Connect the flow cell outlet tubing (C) to the tubing to the drain.

When using Inert cell, UHPLC Inert cell or Low Dispersion Inert cell,
use the supplied coupling 1.6-0.8C PEEK. In this case, connect the
coupling to the cell inlet tubing.

5 Attach the syringe (K) with mobile phase solution for analysis to the syringe
needle, and slowly push the plunger to check for a fluid leak.

If there is a fluid leak, rinse the lenses again, and then tighten the
setscrews again so that they are not loose.

6 Loosen the male nut (A), and remove the syringe needle.

4 Attach the flow cell.

"4.2.2 Installing the Flow Cell" P.104

4.6 Storing the Flow Cell

Before storing the flow cell, pump methanol into it. Then, drain the methanol with the
syringe and dry the inside of the flow cell or fill the flow cell with methanol and seal
the cell before storing it.

116 SPD-40 SPD-40V

 4.7 Replacing the Lamp

4.7 Replacing the Lamp

The SPD-40 has a D2 lamp, and the SPD-40V has a D2 lamp and a tungsten lamp. The
lamps need to be replaced periodically.
As the D2 lamp and W lamp in this detector approach the end of their life, their intensity
decreases and baseline noise increases. Replace the lamps with new ones using the
guaranteed service life as a guide.

n Guaranteed Service Life of the Lamp

Guaranteed service life of the D2 lamp : 2000 hours

The guaranteed service life of the D2 lamp is 2000 hours of use or 1 year from
the date of purchase, whichever is the shorter.

Guaranteed service life of the W lamp : 1200 hours

Average lifetime : 2000 hours

• The guaranteed service life of the W lamp is 1200 hours of use or 1 year from 4
the date of purchase, whichever is the shorter.
• Note that the average lifetime of W lamps is 2000 hours and therefore there
are some lamps broken before 2000 hours of operation.

Before replacing a lamp, turn the detector power switch OFF.

Failing to do so may result in fire, electric shock, or malfunction.

Allow the instrument to cool down adequately before performing the lamp
replacement procedure.
The lamp compartment is extremely hot just after turning off the lamp.

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 4 Maintenance

When replacing the lamp, be careful not to touch the glass portion of the
lamp directly with your fingers.
Wrap the lamp in cotton gauze when carrying it. If the lamp becomes dirty,
wipe it with an ethanol-moistened lens cleaner.

Be careful not to break the lamp.

Do not impart shocks or vibrations to the lamp.

Be sure to install the lamp cover before switching on the lamp.

The lamp cannot be switched on while the lamp cover is removed.
If the lamp cover is removed without switching off the lamp, the lamp is
extinguished automatically.

During replacement work, be sure to keep the surfaces of the mirror and filter
(applicable to only SPC-40V) clean.
A dirty surface of the mirror or filter prevents accurate detection.

Necessary Tools
Phillips-head screwdriver (#2)

Necessary Parts

Part Name Part Type Part No. Remarks

D2 lamp Consumable part 228-73407 －
W lamp Consumable part 228-73443 For SPD-40V only

118 SPD-40 SPD-40V

 4.7 Replacing the Lamp

4.7.1 Removal of Top Cover and Radiating Fin Assembly

1 Turn OFF the main power switch on the back.

2 Unscrew the two top cover installation screws (B), and remove the top cover (A).

The fcover assembly's two screws cannot be removed.

3 Loosen the four screws (D) holding the radiating fin assembly in the lamp
compartment, and remove the fin assembly (C).
The D2 lamp (F), mirror (G), filter (H) (for SPD-40V only) and W lamp (I) (for SPD-40V
only) will now be visible inside the lamp housing (E).

The fin assembly's four screws cannot be removed.

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 4 Maintenance

Lamp installation layout

4.7.2 Deuterium Lamp Replacement

1 Remove the connector (A) of the D2 lamp and then the lead wire (C) from the
clamp (B).

120 SPD-40 SPD-40V

 4.7 Replacing the Lamp

2 Remove the short screw (E) and long screw (F) fastening the D2 lamp and then
remove the D2 lamp.

• The nut on the long screw (G) has been set to a particular position. Do not
loosen or move it.
• Avoid dropping the screws into the detector chassis; it is difficult to retrieve
them. However, be sure to retrieve them if they are dropped.
• If the D2 lamp is tight and it is difficult to remove it, screw in screws in two
screw holes (D) gradually and evenly to remove the D2 lamp from the lamp
housing.

Before replacing a lamp, turn the detector power switch OFF.

Failing to do so may result in fire, electric shock, or malfunction.

Allow the instrument to cool down adequately before performing the lamp
replacement procedure.
The lamp compartment is extremely hot just after turning off the lamp.

Be careful not to break the lamp.

Do not impart shocks or vibrations to the lamp.

During replacement work, be sure to keep the surfaces of the mirror clean.
A dirty surface of the mirror prevents accurate detection.

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 4 Maintenance

3 Fit the new D2 lamp (H) in place, and secure it with the screws.

When positioning the lamp, hold it with gauze to keep the surfaces clean. If
the lamp gets dirty, accurate analysis will not be possible.
Accurate readings cannot be taken when the glass surface is dirty.
Wipe off fingerprints on the glass using a cloth moistened with alcohol.

Be careful not to touch the glass parts directly with your hands when replacing
the lamp.
Wrap the lamp in cotton gauze when carrying it. When the lamp is dirty, wipe
it off with lens paper soaked in ethanol.

122 SPD-40 SPD-40V

 4.7 Replacing the Lamp

4 Put the lead wire (C) in the notch (I) and then fix it with the clamp (B) before
connecting the connector (A) back.

Ensure that the cable (C) is fitted into the notch (I) in the lamp housing, with
the 3-pin connector (A) located outside the housing.
If the cable is not in the notch, it could be broken, resulting in shorting or
lamp failure. (The same applies for the W lamp.)

Be sure to fix the lead wire (C) with the clamp (B).
Failure to do so may cause the lead wire to be broken and result in
malfunction.

5 Replace the fin assembly and top cover of the lamp housing and detector
respectively, and tighten the screws.

Securely tighten the screw of the radiation fin.

A loose screw may worsen the performance.

Attach the top cover in the correct orientation by aligning it with the hollow
of the instrument. (Tighten it with screws.)
Incorrect attachment may cause ultraviolet light to escape.

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 4 Maintenance

n Checking the total illumination time

1 Insert the power plug to the outlet and turn ON the main power switch on the
back.
The initial screen will be displayed in a while.

2 Press [↓] several times to display [D2

LAMP USED TIME].
• "3.7.2 Displaying the VP
Functions" P.52

• "[D2 LAMP USED TIME]" P.54

3 Confirm that the value is 0.

Confirm that "0/2000h" is displayed.

4 Press [CE].
The initial screen is displayed.

4.7.3 Tungsten Lamp Replacement (for SPD-40V Only)

1 Disconnect the 2-pin connector (A) at the end of the W lamp cable.

124 SPD-40 SPD-40V

 4.7 Replacing the Lamp

2 Loosen the three lamp screws (C), and remove the W lamp (B).

When removing the screws, do not drop the screws into the detector chassis. If
you drop it, be sure to remove it.

Before replacing a lamp, turn the detector power switch OFF.

Failing to do so may result in fire, electric shock, or malfunction.

Allow the instrument to cool down adequately before performing the lamp
replacement procedure.
The lamp compartment is extremely hot just after turning off the lamp.

Be careful not to break the lamp.

Do not impart shocks or vibrations to the lamp.

During replacement work, be sure to keep the surfaces of the mirror clean.
A dirty surface of the mirror prevents accurate detection.

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 4 Maintenance

3 Place the new lamp in position in the lamp socket, matching the projection (E)
on the socket to the notch (D) in the lamp flange.

Be careful not to touch the glass parts directly with your hands when replacing
the lamp.
Wrap the lamp in cotton gauze when carrying it. When the lamp is dirty, wipe
it off with lens paper soaked in ethanol.

4 Re-tighten the three lamp screws alternately.

The W lamps are fixed.

126 SPD-40 SPD-40V

 4.7 Replacing the Lamp

5 Put the lead wire (F) in the notch (G) and connect the 2P connector (A) back.

Ensure that the cable is fitted into the notch (G) in the lamp housing, with
the 2-pin connector (A) located outside the housing.
If the cable is not in the notch, it could be broken, resulting in shorting or
lamp failure. (The same applies for the D2 lamp.)

6 Replace the fin assembly and top cover of the lamp housing and detector
respectively, and tighten the screws.

Securely tighten the screw of the radiation fin.

A loose screw may worsen the performance.

Attach the top cover in the correct orientation by aligning it with the hollow
of the instrument. (Tighten it with screws.)
Incorrect attachment may cause ultraviolet light to escape.

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 4 Maintenance

n Resetting the Lamp Operating Time

1 Plug in the detector, and turn the power switch ON.

The initial screen is displayed.

2 Display the [MAINTENANCE] function group of the VP function.

To display the [MAINTENANCE] information group, press [→] in the initial screen and
then move the cursor to [VP] followed by pressing [→] before moving the cursor to
[MAINTENANCE] and pressing [→].

3 Press [↓] twice to display [W LAMP

USED TIME].
"3.7.2 Displaying the VP Functions"
P.52
"[W LAMP USED TIME]" P.54

4 Press [Enter].
The numeric keypad is displayed.

5 Press [0], then [Enter].

The timer will be reset, and the [W LAMP
USED TIME] value will change to [0].

6 Press [CE].
The initial screen is displayed.

n Setting Brightness

1 Display the [PARAMETER] setting

group of the auxiliary function
(FUNCTION) group.
To display the [PARAMETER] setting group,
press [→] three times in the initial screen.

2 Press [↓] repeatedly until [W LAMP

POWER] is displayed.

3 If the [W LAMP POWER] value is above 0, press [Enter], [0], and then [Enter] to
reset the value to 0.

128 SPD-40 SPD-40V

 4.7 Replacing the Lamp

4 Press [CE] to go back to the initial screen.

5 Display the [PARAMETER] setting group of the auxiliary function (FUNCTION)

group.
To display the [PARAMETER] setting group, press [→] three times in the initial screen.
[LAMBDA 1] is displayed.

6 Press [↓] repeatedly until [LAMP] is displayed and then check the lamp setting.

7 When the setting displayed in the

upper left is not "2" (W lamp ON),
press [Enter], [2], and then [Enter] to
set the setting to "2".

8 Press [CE] to return to the initial screen.

4
9 Display the [PARAMETER] setting
group of the auxiliary function
(FUNCTION) group.
To display the [PARAMETER] setting group,
press [→] three times in the initial screen. [LAMBDA 1] is displayed.

10 Press [Enter].
The numeric keypad is displayed.

11 Press [5], [4], [0], and then [Enter].

This sets the wavelength to be measured to 540 nm.
"SETTING WAVELENGTH1 [LAMBDA 1]" P.34

12 Press [CE] to return to the initial screen.

13 Press [↓].
The screen displays the voltages of the
reference light and sample light.
［REF： If the [REF: ***mV] value is at
least "500mV", your are finished.
If the value is lower than "500mV", go on to step 14.

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 4 Maintenance

14 Increase the [W LAMP POWER] value in the order of [1], [2], and [3].
To display [W LAMP POWER], press [→] three times in the initial screen and then press
[↓] several times.
Increase the [W LAMP POWER] value until the [REF: ***mV] value exceeds "500mV".
You are finished when the value exceeds "500mV".

• The [W LAMP POWER] value can be set to a maximum of [9].

"[W LAMP POWER]" P.41

• Dispose of the used W lamp as industrial waste.

4.8 Wavelength Accuracy Calibration

Measure the peak wavelengths in the emission line spectra produced by the deuterium
lamp, and calibrate them to minimize wavelength variance.

Necessary parts

Part Name Part Type Part No. Remarks

Distilled water, methanol,
- - -
or acetonitrile
Syringe, 1 ML Standard accessory 228-57028 -
Syringe needle Standard accessory 228-57027 -

1 Enclose either of water, methanol, or acetonitrile in the cell.

Use the pump to pump water, methanol, or acetonitrile through the flow cell, or fill the
cell with any of these three using a syringe and syringe adapter.
Refer to "4.3 Flow Cell Inspection/Cleaning" P.105 for the proper use of the syringe and
syringe adapter.

2 Make sure there are no air bubbles in the flow cell, and then install the cell into
the detector.

3 Press [CE] repeatedly until the initial

screen is displayed.

130 SPD-40 SPD-40V

 4.8 Wavelength Accuracy Calibration

4 Display the [CALIBRATION] function

group of the VP function.
To display the [CALIBRATION] function
group, press [→] in the initial screen and
then move the cursor to [VP] followed by pressing [→] before moving the cursor to
[CALIBRATION] and pressing [→]. The password entry screen is displayed.

5 Input the password.

The default password is [00000].

6 Press [Enter] and then press [↓] until

[WAVE CALIB] is displayed.

7 Press [Enter].
Wavelength accuracy calibration is run automatically.
4

8 When the automatic calibration of wavelength accuracy is completed, the

measurement of wavelength accuracy is started.
When the measurement of wavelength
accuracy is completed, the screen displays
"CHECK GOOD" and then differences from
241.5 nm and 656.1 nm.
For the measurement of wavelength accuracy, see "[WAVE CALIB]" P.63.

When [CHECK NO GOOD] is

displayed, refer to System Guide
"5.4 Error Message List".

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 4 Maintenance

4.9 Absorbance Accuracy Calibration

Calibrate absorbance accuracy so as to minimize the difference between the absorbance
value displayed on the instrument when the tested absorbance calibration filter is in
place and the absorbance value of the filter.

Necessary parts

Part Name Part Type Part No. Remarks

Filter holder Tool 228-35011-91 －
Absorbance calibration filter
Tool 228-40251-04 －
(certification sheet included)

1 Press [CE] repeatedly until the initial screen is displayed.

2 Display the [PARAMETER] setting

group of the auxiliary function
(FUNCTION) group.
To display the [PARAMETER] setting group,
press [→] three times in the initial screen. [LAMBDA 1] is displayed.

3 Press [↓] repeatedly until [LAMP] is

displayed.

4 If the setting displayed in the upper right is not "1", press [Enter], [1], and then
[Enter].
The setting displayed in the upper right changes to "1" (D2 lamp ON).

5 Press [CE] to return to the initial screen.

6 Display the [CALIBRATION] function

group of the VP function.
To display the [CALIBRATION] function
group, press [→] in the initial screen and
then move the cursor to [VP] followed by pressing [→] before moving the cursor to
[CALIBRATION] and pressing [→]. The password entry screen is displayed.

7 Input the password.

The default password is [00000].

132 SPD-40 SPD-40V

 4.9 Absorbance Accuracy Calibration

8 Press [Enter] and then press [↓] until

[ABS CALIB] is displayed.
[ABS CALIB], [Set FIL HOLDER], and [ABS
CALIB Enter to Calib] will display
alternately.

9 Remove the standard flow cell, attach the filter holder (A) to the detector and
place the cover (B) over the filter holder. Then, press [Enter].

10 The display shows [Input Filter WL].

Enter the wavelength used for
absorbance calibration, and then press
[Enter].

11 After about 5 seconds, when [Insert

Filter] and [Input Filter Abs] are
displayed, remove the cover and then
attach the absorbance calibration filter
before attaching the cover.

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 4 Maintenance

12 Press [Enter].
[Input Filter Abs] is displayed.

13 Input the absorbance given on the certificate for the inputted wavelength and
then press [Enter].

14 The [Calibrating] indication changes to

[Calibrated], and the calibration ends.
Follow the steps below to make sure that
the calibration has ended successfully.

15 Remove the absorbance calibration filter from the filter holder, and attach the
filter holder cover.

16 Press [CE] to return to the initial screen.

17 Display the [PARAMETER] setting group of the auxiliary function (FUNCTION)

group.
To display the [PARAMETER] setting group, press [→] three times in the initial screen.
[LAMBDA 1] is displayed.

18 Press the [Enter] key to display the

numeric keypad. After entering the
wavelength used for calibration, press
the [Enter] key.
"Setting Wavelength1 [LAMBDA 1]" P.34

134 SPD-40 SPD-40V

 4.9 Absorbance Accuracy Calibration

19 Press [CE] to return to the initial screen and then press [ZERO] followed by [Enter]
to check that the displayed absorbance changes to "0".

20 Insert the absorbance calibration filter (C), then attach the cover.

4
21 Record the absorbance value shown on the initial screen.

22 Confirm that the error between the displayed absorbance and the absorbance
value given on the certificate of the absorbance calibration filter is within ±3 %.
This finishes the calibration of the absorbance accuracy.

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 4 Maintenance

4.10 Leak Sensor Calibration

Re-calibration is required if the result of [LEAK SENSOR CHECK] fails the component
validation.
"[LEAK SENSOR CHECK]" P.57

The leak sensor calibration must be performed by the system administrator.

The leak sensor detects leaked mobile phase when the tubing causes the leakage.
When the output value from the sensor exceeds the level set at "threshold
value", an error occurs and temperature control stops.

1 Press [→] on the initial screen.

If the initial screen is not displayed, press [CE] to display the initial screen.

2 Press [↓] once, move the cursor to [VP] and press [→].

3 Press [↓] several times, move the cursor to [CALIBRATION] and press [→].

4 Input the password.

5 Press [↓] several times until [LEAK

THOLD] is displayed.

6 Soak the sensor unit (A) at the bottom

of the leak sensor in water.
The leak sensor value (Actual Level)
increases by detecting water.

7 Read the maximum value of the leak

sensor value (Actual Level).
Example: Here, the maximum value is
[160].

8 Wipe off the moisture around the leak

sensor.
The leak sensor value (Actual Level)
decreases.

When wiping off moisture, do not press the leak sensor. When a sensor unit
touches a wall, the sensor sensitivity is lowered.

136 SPD-40 SPD-40V

 4.11 Exterior Cleaning

9 From the value read in step 7, calculate the threshold value of the leak sensor.
How to obtain the leak sensor threshold:
(Maximum value-100) ´ 0.7 + 100
In the example screen here, [142] is calculated.

10 Press [Enter], input the value

calculated in the step 9, and press
[Enter].
The new "threshold value" is saved.

4.11 Exterior Cleaning

If the instrument cover or front panel becomes dirty, wipe it clean with a soft dry cloth
or tissue paper.
For persistent stains, clean the exterior using the following procedure.

1 Dip a piece of cloth in a dilute neutral detergent and twist firmly to remove
excess liquid. Use this cloth to scrub the soiled area of the exterior surface of
4
the instrument.

2 Dip a piece of cloth into water and twist firmly to remove excess liquid. Use
this cloth to wipe away all the remaining detergent. Use a dry cloth to remove
all moisture from the exterior surface of the instrument.

Do NOT allow spilled water to remain on the instrument surface, and do not
use alcohol or thinner-type solvents to clean the surfaces.
These can cause rusting and discoloration.

SPD-40 SPD-40V 137

 5 Technical Information
5.1 Specifications

5.1.1 Performance Specifications

Item SPD-40 SPD-40V

Light source D2 lamp D2 lamp, W lamp
190 to 1000 nm
190 to 370 nm (deuterium
190 to 700 nm lamp)
Cut-off filter to eliminate 371 to 1000 nm (tungsten
Wavelength range second-order diffraction is lamp)
automatically activated for (Cut-off filter to eliminate
the 371 to 700 nm range. second-order diffraction is
automatically activated for
the 701 to 1000 nm range.)
Spectral bandwidth 8 nm
Wavelength accuracy ±1 nm
Wavelength reproducibility ±0.1 nm*1
Single wavelength mode:
4.0 × 10-6 AU Max.
Single wavelength mode:
(230 nm pumping water
4.0 × 10-6 AU Max.
1 mL/min, response 2 sec)
(230 nm pumping water 1
5.0 × 10-6 AU Max.
mL/min, response 2 sec)
Noise Level (600 nm pumping methanol
Dual wavelength mode*2:
1 mL/min, response 2 sec)
10.0 × 10-6 AU Max.
Dual wavelength mode*2:
(250 nm pumping methanol
10.0 × 10-6 AU Max.
1 mL/min, response 2 sec)
(250 nm pumping methanol
1 mL/min, response 2 sec)
0.1 × 10-3 AU/hour Max.
0.1 × 10-3 AU/hour Max.
(250 nm, 600 nm room
(250 nm, room temperature
Drift temperature constant,
constant, pumping methanol
pumping methanol
1 mL/min)
1 mL/min)
Linearity 2.5 AU (Caffeine, 273 nm)
0.1 × 10-3 AU/℃ Max. (250 nm, pumping methanol 1
Temperature coefficient
mL/min)
Single wavelength mode: 10、20、50、100、200、500、
Sampling time
1000、2000 ms
SCL(CBM)-40に接続した場合
Dual wavelength mode*3: 100、200、500、1000、2000 ms
The value is automatically set according to the sampling
time. For manual setting, the user can choose from the
Response following.
No filtering, 0.05, 0.1, 0.5, 1.0, 1.5, 2.0, 3.0, 6.0, 8.0, 10.0
seconds

138 SPD-40 SPD-40V

 5.1 Specifications

*1 Reproducibility when the wavelength is changed in single wavelength mode.

*2 The setting values of wavelength for dual-wavelength mode are “250 nm” and “280 nm”.
*3 The 100 and 200 ms of dual-wavelength mode are generated by interpolation.

SPD-40 SPD-40V 139

 5 Technical Information

5.1.2 Standard Flow Cell

Item SPD-40 SPD-40V

Cell length 10 mm
Cell volume 12 µL
Cell pressure resistance 12 MPa
Cell connection materials SUS316L, PFA (fluorine resin), Quartz, PEEK
1 to 13
(Using some mobile phases with pH 10 or above for long
pH range periods may damage the flow cell quartz and change its
transmission property.)
"Precautions after Operation" P.18

14 µL：I.D. 0.13mm, length 1040 mm (The length out of the

Inlet tube
cell is 315～340 mm.)
Outlet tube 8 µL：I.D. 0.25 mm, length 160 mm
0 (OFF), 9 to 50 °C, 1 °C step
Cell temperature input range
"[CELL TEMP]" P.39

5.1.3 Detector Unit

Item SPD-40 SPD-40V

Selectable, two wavelengths Selectable, two wavelengths
Measurement
Dual in 190 to 370 nm or 371 to in 190 to 370 nm, 371 to 700
wavelength
wavelength 700 nm range nm or 701 to 1000 nm range
monitor*1 Sampling
0.5 sec
frequency
Can be set between 0.0001 and 2.56 AUFS in 0.0001 AUFS
Range
steps
Zero adjustment Auto zero function, baseline shift function
Polarity switching Possible

140 SPD-40 SPD-40V

 5.1 Specifications

Item SPD-40 SPD-40V

Performs wavelength scanning with flow stopped. Data
Wavelength
stored in three files, one of which stores background.
scanning
Background is subtracted from sample scans. File data is not
function
saved up (lost when power is turned OFF).
Wavelength
1 to 5 nm, selectable in 5 steps
steps
Scanning 10 to 50 nm/sec, settable in 5 steps (according to the
speed wavelength step setting)
Output speed
1, 3, 10 nm/sec
to a recorder
Ratio chromatogram Outputs the absorption ratio of two wavelengths
Time Program Available in detector or by system controller or software
Wavelength (including dual wavelengths), auto zero, range,
Set items marker, response, event, polarity, lamp ON/OFF, loop, cell
temperature, (Recycle valve) AUTO/ON, stop
Number of
steps
Maximum 32 steps
(detector
program)
Output for
10 mV recorder connectors
recorder
6 steps: 0.5, 1, 2, 4, 1.25 and 2.5 AU/V
Output Integrator output connectors output chromatogram in single
Output for wavelength mode or the Ch1 chromatogram in dual 5
integrator wavelength mode. Ratio chromatogram, spectrum, and Ch2
chromatogram are output from the recorder output
connectors.
Lamp hour-meter Records up to 9999 hours
Dimensions 260 (W) × 140 (H) × 500 (D) mm
Mass 11 kg (main unit only)
Operating temperature range 4 °C to 35 °C
Installation Category II
Pollution Degree 2
Installation Environment (IEC)
Altitude 2000 m or lower
Install inside the room.

*1 Noise level in dual wavelength mode varies depending on the wavelength. For example, use
sensitivity 0.005 AUFS max. at 250 nm. Generally, for wavelengths with small optical outputs, noise
level increases accordingly.

SPD-40 SPD-40V 141

 5 Technical Information

n Power Supply

Item Specification

Power Supply Rated

Power
Voltage*1 Breaking
Part No. Consump- Frequency
(indicated on Capacity*5
SPD-40 tion \2
the instrument)
S228-65312-46 100-240 V AC
150 VA 50/60 HZ 50 A
S228-65312-58 (100-240 V)

Power Supply
Power Rated
Voltage*1
Part No. Consump- Frequency Breaking
(indicated on
SPD-40V tion Capacity*2
the instrument)
S228-65313-46 100-240 V AC
150 VA 50/60 HZ 50 A
S228-65313-58 (100-240 V)

*1 Mains supply voltage fluctuations are not to exceed 10 % of the nominal supply voltage.
*2 Connect the instrument to a power outlet that is equipped with a circuit breaker that shuts off
the current at the described value or less.

5.2 Consumable Parts/Maintenance Parts/Replacement Parts

Parts with no description in the model name are common to this series.

• When replacing a part, be sure to use one listed here.

• For how to replace a part, see "4 Maintenance" P.101.

5.2.1 Consumable Parts

No. Part Name Part No. Remarks

1 D2 lamp, L15050 228-73407 Light source
2 W lamp, PCL917YT 228-73443 Light source (SPD-40V only)

5.2.2 Maintenance Parts

No. Part Name Part No. Remarks

1 Gasket, ID2.0, 2PC 228-35097-95 Cell gasket (2 pcs)
2 Lens 228-73281-41 Cell lens

142 SPD-40 SPD-40V

 Index
A LOCAL 45
ABS CALIB 65, 133
AUTO CHECK 58 M
AUX range 23, 37 MAINTENANCE LOG 55

B O
BEEP MODE 48 OPERATION LOG 55
BL OFFFSET ITG 38 OPERATION MODE 61, 92
BL OFFSET REC 38
BRIGHTNESS 48 P
PARAMETER LOCK 61
C PARAMETER 31, 76
CAFFEINE CHECK 59 Parameters 21, 61
CBM PARAMETER 63, 67 PART REPLACEMENT 55
CELL ID 29 PARTS MGMT TOOL 62
CELL READY 47 PASSWORD SETTING 62
CELL TEMP 39 PLOT SPEED 42
CH1: SMP/REF 28 Polarity 23, 37
CH2: SMP/REF 29 POWER BUTTON 45
Controlled Cell Temperature 25 PROG TIME 30

D R
D2 ENERGY 64 RANGE 37
D2 LAMP USED TIME 29, 54, 124 RATIO RANGE 39, 81
D2 TIME 63 RATIO THR 40
DATE 57 REC MODE 38
DATE FORMAT 61 Recorder setting 24
Direct Key 14, 47, 49 Recycle valve 25
DISP OFF TIME 48 Response 23, 35, 77

E S
EDIT 49 SCAN BEGIN 41
ERROR LOG 55 SCAN END 41
EVENT1 39, 99 SCAN FILE 41, 85
EVENT2 39, 99 SCAN STEP 42
SELECT EVENT1 46
I SELECT EVENT2 46
INITIAL 241nmCHK 65 SERIAL NUMBER 52
Initial Screen 28 SPC PLOT 44
INITIALIZE PARAM 61 SV DELAY TIME 40
SV LEVEL 40
K SYSTEM 45
KEY CLOSE 48
T
L TIME 57
LAMBDA 1 34 TOTAL OP TIME 30, 54
LAMBDA 2 35
LAMP 36
U
LEAK SENSOR CHECK 57 UTILITY 47
LEAK THOLD 63
LINK ADDRESS 45

SPD-40 SPD-40V 143

V
version i, 53

W
W ENERGY 64
W LAMP POWER 41, 128
W LAMP USED TIME 29, 54
W TIME 64
WAVE CALIB 63, 131
WAVE CHECK 43
WAVE MODE 35
WAVE SCAN 44
Wavelength 22

き
Change the setting when performing the
wavelength accuracy test with manual wavelength
change 65

144 SPD-40 SPD-40V