Magnesium XL FS*

Diagnostic reagent for quantitative in vitro determination of magnesium in serum, plasma, cerebrospinal
fluid or urine on photometric systems

Order Information 2. In very rare cases, samples of patients with gammopathy

might give falsified results [8].
Cat. No. Kit size
3. Please refer to the safety data sheets and take the necessary
1 4610 99 10 021 R 6x 25 mL
precautions for the use of laboratory reagents. For diagnostic
1 4610 99 10 026 R 6x 100 mL
purposes, the results should always be assessed with the
1 4610 99 10 704 R 8x 50 mL
patient’s medical history, clinical examinations and other
1 4610 99 10 930 R 6x 20 mL
findings.
1 4610 99 90 314 R 12 x 25 mL
4. For professional use only!
Summary Waste Management
Deficiency of magnesium is a quite common disorder, which can be Please refer to local legal requirements.
caused by malnutrition, malabsorption, renal loss and
endocrinological disturbances. Complications associated with Reagent Preparation
decreased magnesium concentrations are neuromuscular irritability The reagent is ready to use.
(e.g. tremor, seizures) and cardiac symptoms (e.g. tachycardia,
arrhythmia). Decreased magnesium concentrations are often Materials required but not provided
related to decreased calcium and potassium levels, taking into NaCl solution 9 g/L
account that hypomagnesemia may be the primary cause of General laboratory equipment
hypocalcemia. Elevated magnesium values can be observed in
dehydration, renal disorders and after intake of excessive amounts Specimen
of antacids and can be associated with weakness of reflexes and Serum, plasma, cerebrospinal fluid (CSF) or urine
low blood pressure [1,2]. Do not use EDTA plasma.
Method Stability [3]:
in serum/plasma: 7 days at 20 – 25°C
Photometric test using xylidyl blue
7 days at 4 – 8°C
Principle 1 year at –20°C
in urine: 3 days at 20 – 25°C
Magnesium ions form a purple colored complex with xylidyl blue in 3 days at 4 – 8°C
alkaline solution. In presence of GEDTA, which complexes calcium 1 year at –20°C
ions, the reaction is specific. The intensity of the purple color is
proportional to the magnesium concentration. Acidify urine with some drops of conc. HCl to pH 3-4, then dilute
1+4 with dist. water; multiply the result by 5.
Reagents Freeze only once!
Discard contaminated specimens!
Components and Concentrations
Reagent: Assay Procedure
Ethanolamine pH 11.0 750 mmol/L
GEDTA (Glycoletherdiamine- 60 µmol/L Application sheets for automated systems are available on
tetraacetic acid) request.
Xylidyl blue 110 µmol/L Wavelength 520 nm, Hg 546 nm, 500 - 550 nm
(Increase of absorbance)
Storage Instructions and Reagent Stability
628 nm, Hg 623 nm, 570 - 650 nm
Reagent is stable up to the end of the indicated month of expiry, if (Decrease of absorbance)
stored at 2 – 8°C and contamination is avoided. Do not freeze the Optical path 1 cm
reagent! Temperature 20 – 25°C/37°C
Warnings and Precautions Measurement Against reagent blank
1. Reagent: Danger. Contains: Ethanolamine. H315 Causes
Blank Sample/Calibrator
skin irritation. H318 Causes serious eye damage. P264 Wash
Sample/Calibrator - 10 µL
hands and face thoroughly after handling. P280 Wear
Dist. water 10 µL -
protective gloves/protective clothing/eye protection.
Reagent 1000 µL 1000 µL
P305+P351+P338 If in eyes: Rinse cautiously with water for
Mix and read absorbance against blank after 5-60 min. at 20 –
several minutes. Remove contact lenses, if present and easy
25°C/37°C.
to do. Continue rinsing. P310 Immediately call a poison center
or doctor/physician.

Magnesium XL FS – Page 1 * fluid stable

Calculation Reference Range [1,6]
With calibrator Serum/Plasma:
Neonates 1.2 – 2.6 mg/dL (0.48 – 1.05 mmol/L)
Magnesium [mg/dL] = A Sample x Conc. Cal. [mg/dL] Children 1.5 – 2.3 mg/dL (0.60 – 0.95 mmol/L)
A Cal. Women 1.9 – 2.5 mg/dL (0.77 – 1.03 mmol/L)
Men 1.8 – 2.6 mg/dL (0.73 – 1.06 mmol/L)
Conversion factor Urine: 73 – 122 mg/24 h (3 – 5 mmol/24 h)
Magnesium [mg/dL] x 0.4114 = Magnesium [mmol/L] CSF: 2.1 – 3.3 mg/dL (0.85 – 1.35 mmol/L)

Calibrators and Controls Each laboratory should check if the reference ranges are
For the calibration of automated photometric systems, DiaSys transferable to its own patient population and determine own
TruCal U calibrator is recommended. The assigned values of the reference ranges if necessary.
calibrator have been made traceable to the reference method
Atomic Absorption Spectrometry (AAS). Magnesium Standard FS Literature
may be used alternatively for calibration. DiaSys TruLab N and P or
1. Thomas L. Clinical Laboratory Diagnostics. 1st ed. Frankfurt:
TruLab Urine controls should be assayed for internal quality control.
TH-Books Verlagsgesellschaft; 1998. p. 231-41.
Each laboratory should establish corrective actions in case of
2. Endres DB, Rude RK. Mineral and bone metabolism. In: Burtis
deviations in control recovery.
CA, Ashwood ER, editors. Tietz Textbook of Clinical
Cat. No. Kit size Chemistry. 3rd ed. Philadelphia: W.B Saunders Company;
TruCal U 5 9100 99 10 063 20 x 3 mL 1999. p. 1395–1457.
5 9100 99 10 064 6 x 3 mL 3. Guder WG, Zatwa B et al. The quality of Diagnostic Samples.
TruLab N 5 9000 99 10 062 20 x 5 mL 1st ed. Darmstadt: Git Verlag, 2001: 38-39, 50-51.
5 9000 99 10 061 6 x 5 mL 4. Mann CK, Yoe JH. Spectrophotometric determination of
TruLab P 5 9050 99 10 062 20 x 5 mL magnesium with 1-Azo-2-hydroxy-3-(2.4-dimethyl-
5 9050 99 10 061 6 x 5 mL carboxanilido)-naphthalene-1’-(2-hydroxybenzene). Anal Chim
TruLab Urine Level 1 5 9170 99 10 062 20 x 5 mL Acta 1957; 16: 155-60.
5 9170 99 10 061 6 x 5 mL 5. Bohoun C. Microdosage du magnesium dans divers milieux
TruLab Urine Level 2 5 9180 99 10 062 20 x 5 mL biologiques. Clin Chim Acta 1962;7:811-7.
5 9180 99 10 061 6 x 5 mL 6. Sitzmann FC. Normalwerte. München: Hans Marseille Verlag
Magnesium Standard FS 1 4600 99 10 030 6 x 3 mL GmbH: 1986. p. 166.
7. Young DS. Effects of Drugs on Clinical Laboratory Tests. 5th
ed. Volume 1 and 2. Washington, DC: The American
Performance Characteristics Association for Clinical Chemistry Press 2000.
8. Bakker AJ, Mücke M. Gammopathy interference in clinical
Measuring range chemistry assays: Mechanisms, detection and prevention. Clin
The test has been developed to determine magnesium Chem Lab Med 2007; 45(9): 1240–1243.
concentrations within a measuring range from 0.05 - 5 mg/dL
(0.02 – 2.05 mmol/L). When values exceed this range samples
should be diluted 1+4 with NaCl solution (9 g/L) and the result
Manufacturer
DiaSys Diagnostic Systems GmbH
multiplied by 5. IVD
Alte Strasse 9 65558 Holzheim Germany
Specificity/Interferences
No interference was observed by ascorbic acid up to 30 mg/dL,
bilirubin up to 40 mg/dL, lipemia up to 2,000 mg/dL triglycerides and
calcium up to 25 mg/dL. Hemoglobin interferes because
magnesium is released by erythrocytes. For further information on
interfering substances refer to Young DS [7].

Sensitivity/Limit of Detection
The lower limit of detection is 0.05 mg/dL (0.02 mmol/L).

Precision (at 37°C)

Intra-assay Mean SD CV
n = 20 [mg/dL] [mg/dL] [%]
Sample 1 1.88 0.02 0.92
Sample 2 2.34 0.02 0.87
Sample 3 4.02 0.03 0.83

Inter-assay Mean SD CV
n = 20 [mg/dL] [mg/dL] [%]
Sample 1 1.84 0.02 1.09
Sample 2 2.38 0.03 1.12
Sample 3 4.11 0.06 1.43

Method Comparison
A comparison of DiaSys Magnesium XL FS (y) with a commercially
available test (x) using 81 samples gave following results:
y = 1.01 x – 0.03 mg/dL; r= 0.999.

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