Chapter : Marks : 45

Time : 2hr 41
1)Molecular Basis of Inheritance
Negative Marks : 0

1) The process of copying genetic information from one strand of DNA to RNA is termed
as _______.
A) replication B) transcription C) translation D) reverse transcription

2) Which of the following statements is the most appropriate for sickle cell anaemia?
A) It cannot be treated with iron supplements. B) It is a molecular disease.
C) It confers resistance to acquiring malaria. D) All of the above

3) The promoter site and the terminator site for transcription are located at
A) 3' (downstream) end and 5' (upstream) end, respectively of the transcription unit
B) 5' (upstream) end and 3' (downstream) end, respectively of the transcription unit
C) the 5' (upstream) end D) the 3' (downstream) end

4) Find out the wrong sentence about heterochromatin.

A) It is densely packed. B) It stains dark. C) It is transcriptionally active.
D) It is late replicating.

5) To prove that DNA is the genetic material, which radioactive isotopes were used by
Hershey and Chase (1952) in their experiments?
35 15 32 35 35 15 14 15
A) S and N B) P and S C) P and N D) N and N

6) The given figure represents the process of transcription in bacteria. Select the option
which correctly labels A, B and C.

A) A - DNA, B - RNA, C - Promoter B) A - RNA, B - RNA polymerase, C - Rho factor

C) A - RNA, B - RNA polymerase, C - Sigma factor
 D) A - DNA, B - DNA polymerase, C - RNA

7) Given diagram represents the components of a transcription unit. Select the correct
answer regarding it.

A) A - Terminator; B - Promoter; C - Template strand; D - Coding strand

B) A - Promoter; B - Terminator; C - Coding strand; D - Template strand
C) A - Promoter; B - terminator; C - Template strand; D - Coding strand
D) A - Terminator; B - Promoter; C - Coding strand; D - Template strand

8) You have created a fusion between trp operon and lac operon which encodes the
enzymes for tryptophan biosynthesis, under the regulatory control of the lac operator.
Under which of the following conditions will tryptophan synthase be induced in the
strain that carries the chimeric operator fused operons?
A) Only when both lactose and glucose are absent.
B) Only when both lactose and glucose are present.
C) Only when lactose is absent and glucose is present.
D) Only when lactose is present and glucose is absent.

9) One of the following is true with respect to AUG

A) It codes for methionine only B) It is also an initiation codon
C) It codes for methionine in both prokaryotes and eukaryotes D) All of the above

10) In an animal cell, the processes of transcription and translation occur in

A) nucleus only B) cytoplasm only C) nucleus and cytoplasm respectively
D) endoplasmic reticulum

11) The year 2003 was celebrated as the 50th anniversary of discovery of
A) transposons by Barbara Mc Clintock B) structure of DNA by Watson and Crick
C) Mendels laws of inheritance D) biotechnology by Kary Mullis

12) Select the incorrectly matched pair.

A) Initiation codons - AUG, GUG B) Stop codons - UAA, UAG, UGA
C) Methionine - AUG D) Anticodons - mRNA

13) Amino acid acceptor end of tRNA lies at

A) 5' end B) 3' end C) TΨC loop D) DHU loop

14) The amino acid attaches to the tRNA at its

A) 5'-end B) 3'-end C) Anticodon site D) DHU loop
15) Match Column-I with Column-II and select the correct option from the codes given
below.
Column1 Column2
1. Sigma factor a) 5' - 3'
2. Capping b) Initiation
3. Tailing c) Termination
4. Coding strand d) 5' end
e) 3' end
A)1:e;2:b;3:a;4:d B)1:b;2:a;3:d;4:e C)1:b;2:e;3:a;4:d D)1:b;2:d;3:e;4:a

16) Methyl guanosine triphosphate is added to the 5 end of hnRNA in a process of

A) splicing B) capping C) tailing D) none of these

17) Which was the last human chromosome to be completely sequenced?

A) Chromosome 1 B) Chromosome 11 C) Chromosome 21 D) Chromosome X

18) First experimental proof for semi-conservative DNA replication was shown in
A) Streptococcus pneumoniae B) Escherichia coli C) Neurospora crassa
D) Rattus rattus

Comprehension Passage for Q.19 to Q. 20

Direction: Read the sequence of nucleotides in the given segment of mRNA and the
respective amino acid sequence in the polypeptide chain to answer.

19) Nucleotide sequence of the DNA strand from which this mRNA was transcribed is
A) TAC AAA TAC GGA CAA AGA ATT B) AUG UUU AUG CCU GUU UCU UAA
C) UAC AAA UAC GGA CAA AGA AUU D) ATG TTT ATG CCT GTT TCT TAA
20) Which codons respectively code for proline and valine amino acids in the given
polypeptide chain, respectively?
A) CCU and GUU B) GUU and UCU C) UCU and UAA D) GUU and CCU

21) Sickle cell anaemia results from a single base substitution in a gene, thus it is an
example of
A) point mutation B) frame-shift mutation C) silent mutation D) both (1) and (2)

22) Which of the following are the functions of RNA?

A) It is a carrier of genetic information from DNA to ribosomes synthesising
polypeptides.
B) It carries amino acids to ribosomes. C) It is a constituent component of ribosomes.
D) All of the above

23) Select the incorrect statement regarding DNA replication.

 A) Leading strand is formed in 5' → 3' direction.
B) Okazaki fragments are formed in 5' → 3' direction.
C) DNA polymerase catalyse polymersation in 5' → 3' direction.
D) DNA polymerase catalyses polymerisation in 3' → 5' direction.

24) To initiate translation, the mRNA first binds to

A) The smaller ribosomal sub-unit B) The larger ribosomal sub-unit
C) The whole ribosome D) No such specificity exists

25) Given below are the steps of protein synthesis. Arrange them in correct sequence and
select the correct option. (i) Codon-anticodon reaction between mRNA and aminoacyl
tRNA complex. (ii) Attachment of mRNA and smaller sub-unit of ribosome. (iii)
Charging or Aminoacylation of tRNA. (iv) Attachment of larger sub-unit of ribosome to
the mRN A − tRN A Met complex (v) Linking of adjacent amino acids. (vi) Formation of
polypeptide chain.
A) (ii) → (i) → (iii) → (v) → (iv) → (vi) B) (v) → (ii) → (i) → (iii) → (iv) → (vi)
C) (iii) → (ii) → (iv) → (i) → (v) → (vi) D) (iii) → (ii) → (i) → (iv) → (v) → (vi)

26)

Refer to the diagram which shows the synthesis of part of a protein molecule. Which of
the following is the first part of the protein molecule that would be translated from
mRN A 2 ?

A) AA 4 – AA 2 – AA 7 – AA 6 B) AA 6 – AA 7 – AA 2 – AA 4 C) AA 3 – AA 1 – AA 6 – AA 8

D) AA 8 – AA 5 – AA 1 – AA 3

27) Match Column-I with Column-II and select the correct option from the codes given
below.
Column1 Column2
1. F. Meischer a) DNA double helix
2. Griffith b) Nuclein
3. Hershey and Chase c) S. pneumoniae
 4. Watson and Crick d) Bacteriophages
5. Wilkins and Franklin e) X-ray diffraction studies
A)1:b;2:c;3:d;4:a;5:e B)1:c;2:d;3:e;4:a;5:b C)1:b;2:e;3:c;4:d;5:a D)1:a;2:b;3:d;4:e;5:c

28) Match Column-I with Column-II and select the correct option from the codes given
below.
Column1 Column2
1. Alec Jeffreys a) Lac operon
2. F. Sanger b) Automated DNA sequencers
3. Jacob and Monod c) DNA finger printing
4. Avery, Mac Leod and Mc Carty d) Transforming principle
A)1:c;2:b;3:a;4:d B)1:b;2:a;3:c;4:d C)1:c;2:b;3:d;4:a D)1:d;2:c;3:a;4:b

29) Match Column-I with Column-II and select the correct option from the codes given
below.
Column1 Column2
1. UUU a) Serine
2. GGG b) Methionine
3. UCU c) Phenylalanine
4. CCC d) Glycine
5. AUG e) Proline
A)1:a;2:d;3:c;4:e;5:b B)1:b;2:d;3:c;4:a;5:e C)1:c;2:a;3:d;4:b;5:e D)1:c;2:d;3:a;4:e;5:b

30) Estimated number of genes in human beings is

A) 3,000 B) 80,000 C) 20,500 D) 3 × 10
9

31) Which of the following pairs is incorrectly matched?

A) Purines - Adenine and Guanine B) Pyrimidines - Cytosine and Uracil
C) Nucleosides - Adenosine and Thymidine D) DNA - Basic biomolecule

32) A nucleoside differs from a nucleotide. It lacks the

A) base B) sugar C) phosphate group D) hydroxyl group

33) Refer the given figure of nucleosome and select the option that correctly identifies the
parts A, B and C.

A) A− DNA; B− Histone octamer; C − H 1 histone

B) A− Histone octamer; B − H 1 hisotne; C− DNA

 C) A− Histone octamer; B− DNA; C − H 1 histone
D) A− DNA; B − H 1 histone; C− Histone octamer

34) Match Column-I with column-II and select the correct option from the codes given
below.
Column1 Column2
1. Griffith a) Lac operon
2. Hershey and Chase b) Semi-conservative DNA replication
3. Messelson and Stahl c) Transduction
4. Jacob and Monod d) Transformation
A)1:b;2:c;3:a;4:d B)1:c;2:d;3:a;4:b C)1:d;2:c;3:b;4:a D)1:a;2:c;3:b;4:d

35) Match Column-I with Column-II and select the correct option from the codes given
below.
Column1 Column2
1. Operator site a) Binding site for RNA polymerase
2. Promoter site b) Binding site for repressor molecule
3. Regulator gene c) Codes for protein/enzyme
4. Structural gene d) Codes for repressor molecule
A)1:b;2:a;3:d;4:c B)1:c;2:d;3:b;4:a C)1:d;2:a;3:c;4:b D)1:d;2:b;3:a;4:c

36) The fully processed hnRNA is called as (i) and is transported out of the (ii) into the (iii)
for translation.
A) (i) mRNA, (ii) nucleus, (iii) cytoplasm B) (i) mRNA, (ii) cytoplasm, (iii) nucleus
C) (i) tRNA, (ii) cytoplasm, (iii) nucleus D) (i) tRNA, (ii) nucleus, (iii) cytoplasm

37) The fact that a purine base always paired through hydrogen bonds with a pyrimidine
base leads to, in the DNA double helix
A) the antiparallel nature B) the semiconservative nature
C) uniform width throughout DNA D) uniform length in all DNA

38) The first genetic material could be

A) protein B) carbohydrates C) DNA D) RNA

39) Discontinuous synthesis of DNA occurs in one strand, because

A) DNA molecule being synthesised is very long
B) DNA dependent DNA polymearse catalyses polymerisation only in one direction
(5' → 3')
C) it is a more efficient process D) DNA ligase has to have a role

40) Which out of the following statements is incorrect?

A) Genetic code is ambiguous. B) Genetic code is degenerate.
C) Genetic code is universal. D) Genetic code is non-overlapping.
41) The structures in chromatin seen as 'beads-on-string' when viewed under electron
microscope are called
A) nucleotides B) nucleosides C) histone octamers D) nucleosomes

42) The process of transformation is not affected by which of the following enzymes? A.
Dnase B. Rnase C. Peptidase D. Lipase
A) A, B B) A, B, C, D C) B, C, D D) A, B, C

43) If the sequence of bases in DNA is GCTTAGGCAA then the sequence of bases in its
transcript will be
A) GCTTAGGCAA B) CGAATCCGTT C) CGAAUCCGUU D) AACGGAUUCG

44) The structural genes, in eukaryotes, possess coding and non-coding sequences called as
(i) and (ii) respectively.
A) (i) promoter, (ii) operator B) (i) introns, (ii) exons C) (i) exons, (ii) introns
D) (i) enhancer, (ii) silencer

45) Match Column-I with Column-II and select the correctoption from the codes given
below.
Column1 Column2
1. Translation a) Aminoacyl tRNA synthetase
2. Transcription b) Okazaki fragments
3. DNA replication c) RNA polymerase
A)1:b;2:c;3:a B)1:b;2:a;3:c C)1:a;2:b;3:c D)1:a;2:c;3:b