High Performance Thin Layer Chromatography (HPTLC)
• In HP-TLC, mixtures are resolved into their individual components,
using a thin layer of adsorbent, coated on a “plate”.
• Unique features of HPTLC are that it is the fastest, simplest, most
economical and flexible, “visible” technique which can analyse in
parallel more than 100 samples. It is risk free and multiple detections
can be made without repeating chromatogram.
• It is several times faster and economical than HPLC, another liquid
chromatography technique.
• HPTLC is used for the analysis of non-volatile organics such as
pharmaceuticals, APIs, botanicals, forensics, foods, speciality
chemicals, etc. for establishing purity, impurities fingerprint.
Principle- As like TLC
Applications- HPTLC
HPTLC - Forensic Analysis
• Forensic analysis is the multi-disciplinary application of scientific
knowledge and sophisticated instruments for investigating crime
related materials and biological samples. A frequent but challenging
aspect of forensic toxicology is the identification of unknown
poisonous substances in lethal intoxication cases. HPTLC offers
identification as well as qualitative and quantitative analysis for toxic
substances, CAMAG HPTLC offers rapid identification of such toxins
for antidote administration.
HPTLC - Food Analysis
• Foods usually originate as botanical products and therefore are
naturally variable as well as complex. HPTLC can confirm identities of
complex mixtures as well as detect adulteration. HPTLC is the first
method of analysis because it is simple, risk free, fast, economical and
analyses 100-120 samples a day, without producing much waste.
HPTLC Herbal Applications
• HPTLC Fingerprint is a technique adopted by the US & European
Pharmacopoeias recently for the purpose of identification of
“botanical materials”, all of which are very complex in nature. HPTLC
Fingerprint is the representation of the phytochemical composition of
a plant extract or formulation, in the form of a conventional image i.e.
a photograph. Fingerprint can also be used to monitor batch to batch
consistency and stability studies of herbal medicines, dietary
supplements etc
HPTLC – Speciality Chemicals Analysis
• High Performance Thin Layer Chromatography (HPTLC) is a valuable
tool to check purity, impurity of any non-volatile organic industrial
materials such as dyes, surfactants, pesticides, perfumery
compounds, intermediates etc. It is far simpler, cheaper and easier to
understand than other similar methods of analysis.
• Chemical reactions can be studied very quickly e.g. within 2 hours.
Complex mixtures like biological samples, reaction mixtures,
fermentation broth can be easily chromatographed without much
sample preparation.
HPTLC Dyes & Intermediates Analysis
• Dyes and intermediates are non-volatile organic substances and so
best suited for HPTLC analysis. HPTLC analysis is very low cost and
results are “visible”. Coloured substances are therefore particularly
ideal for HPTLC analysis.
• HPTLC enables comparison of different samples or with standard,
analysis of competition samples apart from purity and impurity
determination. All kinds of optical brightner, intermediates, dyes,
banned amines can be analysed by HPTLC.
HPTLC - Biotechnology Applications
• HPTLC is a most versatile analytical technique which offers a great
separation power using precise sample application,
software-controlled chromatographic steps, chromatogram
development and scanning and photo documentation.
• Separated samples can be visually checked on the plate is an unique
aspect of HPTLC. Biotechnology industry is considered as one of the
most research-intensive sectors in the world. Therefore, shorter
analysis time, low sample analysis cost per sample, minimal
contamination possibilities and reliable accurate results are essential
which are provided by HPTLC.
HPTLC-Cosmetics Analysis
• Sunscreen products are widely used across the globe due to their
benefits of preventing skin from tanning and sunburns. The UV filters
used in such formulations protect the skin from harmful UV rays by
forming a thin, protective layer on the skin thereby blocking or
absorbing UV rays from penetrating the skin.
• The safety of such products is of vital importance since they tend to
interact with the biological matrices. In order to shield the consumer
health from any harmful effects, HPTLC technique can be employed
for the detection of such filters, the identity of which can be further
confirmed by mass spectrometer.
Thank you. Any questions ???
