4/5/22, 1:20 PM Bright Field Microscope: Definition, Parts, Working Principle, Application.
LightPath of Brightfield Microscope
A stained specimen or sample is placed over the specimen stage.
A condenser lens containing an aperture diaphragm is located under the
stage, will focus the light ray on the sample or specimen.
The light rays will pass through the specimen sample and then it will be
collected by an objective lens located over the stage.
The objective lens will form a magnified image of the specimen and then
transmit it to the eyepiece, where viewers will observe a dark image against
the brightfield.
During the transmission through the specimen, some of the light rays are
absorbed by the stains, pigmentation, or dense areas of the specimen.
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Parts of Brightfield Microscope
Figure: Bright Field Microscope
A brightfield microscope is consists of these following parts (follow my previous
notes, there I have already discussed all the parts with their functions);
1. The top portion of brightfield microscope is known as the head.
2. The holding portion of the microscope is known as Arm. Which we used to
carry the microscope from one place to another. This portion provides
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4/5/22, 1:20 PM Bright Field Microscope: Definition, Parts, Working Principle, Application.
support to the both optical and mechanical components.
3. The bottom portion of the microscope, which holds the arm and other
components is known as Base.
4. Two lens were located at the top portion of a brightfield microscope, which
are known as the eyepiece or ocular lens. Viewers observe the image
through it.
5. A long tube connected the both ocular lens and objective lens, called as body
tube.
6. An objective lens is located under the eyepiece. This lens helps to magnify
the image. There are present different types of objective lenses, such as 40x,
10x, 100x, etc.
7. A Nosepiece is located under the head. It contains different types of
Objective lens.
8. The Stage is located under the objective lens. The specimen is placed over
the stage.
9. The stage clips are located over the specimen stage. It holds the specimen
slide.
10. There are present different types of objective lenses, they vary based on their
magnification power.
11. A condenser lens is located under the stage. It focuses the light rays on the
specimen.
12. A hole is located over the stage, called the aperture. It pass the light rays. It
also controls the density of light rays.
13. Bright-fields microscope also contains two focusing knobs, called fine
adjustment knob and the coarse adjustment knob. These knobs help to
Share adjust the focus of objective lens.
14. This microscope also contains a stage knob, which controls the stage.
Magnification of Bright-Field Microscope
The magnification power of a brightfield microscope can be calculated by ;
Total Magnification power = Magnification of the objective lens x
Instruments
Get notes about Magnification of the eyepiece
different laboratory
isntruments. Ex. The magnification power of an objective lens is 40x and 10x for the eyepiece,
Microscope then the total magnification power of this bright-field microscope will be 40 x
Microscope, instrument 10= 400x.
that produces enlarged
images of small objects,
allowing the observer …
Application of Bright-Field Microscope
1. Used in blood counting.
0
2. Used to examine the bacterial cells.
3. Used to examine the fungal cells.
4. It also used in forensic laboratories.
5. Used in agricultural laboratories.
6. Used to study plant cells.
Advantages of Bright-Field Microscope
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