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Gram Staining and Microbial Identification

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Swapnil Das
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29 views15 pages

Gram Staining and Microbial Identification

Uploaded by

Swapnil Das
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
Available Formats
Download as PDF, TXT or read online on Scribd

GRAM Staining :

1. Staphylococcus Aurues
 Blood Agar
 Circular, convex, opaque, smooth surface, white in colour
 Non motile
 Gram +ve coccus
 Catalase +ve, Coagulase +ve
2. Bacillus Subtilis
 Nutrient Agar
 Dry, flat, irregular, with lobate margin, white in colour
 Gram +ve bacillus
 Motile
 Catalase +ve
3. E. Coli
 MacConkey Agar
 Circular, moist, smooth with entire margin, pink, non mucoid, pink
 Gram –ve bacillus
 Motile
 Catalase +ve
 Ferment all sugar except sucrose
 Indole +ve , Citrate –ve, Urease –ve
4. Klebsiella sp.
 MacConkey Agar
 Circular, moist, smooth with entire margin, pink to red, mucoid
 Gram –ve bacillus
 Non motile
 Ferment all sugars
 Catalase +ve
 Indole -ve , Citrate +ve, Urease +ve
5. Pseudomas sp.
 MacConkey Agar
 Pale or colourless
 Gram –ve bacillus
 Earthy smell
 Motile
 Ferments glucose
 Motile
 Catalase +ve
 Oxidase +ve
6. Proteus
 MacConkey Agar
 Smooth, pale or colourless
 Fishy smell
 Gram –ve bacillus
 Motile
 Ferment only glucose
 Catalase +ve
 Indole +ve
Spotting(Microscopy)
1. Aspergillus
2. Candida

3. Microbacterium leprae
4. Gram –ve cocci (diplococcus)

5. Gram +ve cocci


6. Malarial parasite(ring form)
7. Microfilarae

Spotting (specimen) :
1. Ascaris
2. Fasciolopsis buski
3. Toxocara canis
 Host : Dog
 Location : Small intestine
4. Fasciola hepatica
 Liver fluke
 Infective form : Metacercariae
 Definitive Host : Human
 Secondary Host : Snail
Spotting (Instruments) :
1. Candle filter
 Used for purification of vaccines
 Purification of water for drinking and industrial purpose
2. Sintered glass filter
 Used for purification of drinking water and vaccines
3. Seitz filter
 Used for purification of hydatid fluid
4. Plastic disposable sterile syringe
5. Sterile cotton swab with a stick in a test tube
 Vagina swab, nasal swab etc.
6. Sterile empty test tube
 Used for collection of Urine
7. Blood culture bottle containing Glucose Broth
Different Medium :
1. Nutrient agar
 White colour
 Peptone water, meat extract(1%)
2. Blood agar
 Red colour
 Blood, agar(2-3%), peptone water, meat extract
3. MacConkey’s agar
 Pink colour
 Peptone, lactose, agar, sodium taurocholate and neutral red
4. Cystone Lactose Electrolyte Deficient Agar/ CLED Agar
 Light green
 Isolation and differentiation of urinary organisms like E.Coli, Klebsiella sp.
5. Thiosulphate Citrate Bile Salt Agar/ TCBS Agar
 Dark green
 Used for selective isolation of vibrio cholerae
6. Sabouraud Dextroxe Agar/ SDA
 Slight yellow
 Used for isolation of dermatophytes

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