Section A: Basic Understanding (15 Questions)

1. Which of the following microorganisms is used in the production of alcohol?

a) Amoeba
b) Yeast
c) Paramecium
d) Algae
2. Name the microorganism that causes malaria.
a) Plasmodium
b) Salmonella
c) Rhizobium
d) Vibrio cholerae
3. Which of the following diseases is caused by a virus?
a) Tuberculosis
b) Polio
c) Typhoid
d) Cholera
4. Nitrogen-fixing bacteria are mostly found in the:
a) Leaves of plants
b) Roots of legumes
c) Stem of crops
d) Flowers of plants
5. Which microorganism is used in the preparation of curd?
a) Lactobacillus
b) Saccharomyces
c) Rhizobium
d) Penicillium
6. Penicillin is obtained from:
a) Yeast
b) Bacteria
c) Fungus
d) Algae
7. The process of converting sugar into alcohol is known as:
a) Fermentation
b) Pasteurization
c) Respiration
d) Photosynthesis
8. Which microorganism is responsible for causing AIDS?
a) HIV
b) HBV
c) H1N1
d) HPV
9. Which of the following is used as a biofertilizer?
a) Yeast
b) Rhizobium
c) Penicillium
d) Amoeba
10. Blue-green algae fix atmospheric nitrogen. They are also known as:
a) Fungi
b) Cyanobacteria
 c) Protozoa
d) Virus
11. Which microorganism is unicellular?
a) Fungi
b) Bacteria
c) Algae
d) Virus
12. Antibiotics are effective against:
a) Viruses
b) Bacteria
c) Fungi
d) All microorganisms
13. Vaccines work by stimulating the production of:
a) Antibodies
b) Pathogens
c) Antigens
d) Viruses
14. The microorganism responsible for the spoilage of bread is:
a) Bacteria
b) Virus
c) Protozoa
d) Fungus
15. Polio is caused by a:
a) Bacteria
b) Virus
c) Protozoa
d) Fungus

Section B: Application-Based Questions (20 Questions)

16. Why are algae considered both beneficial and harmful?
a) They provide oxygen but block waterways.
b) They are edible but cause diseases.
c) They are a biofertilizer but cause infections.
d) They are symbiotic but pathogenic.
17. Bread dough rises due to the action of:
a) Rhizobium
b) Yeast
c) Amoeba
d) Algae
18. Which microorganism is used in the production of antibiotics?
a) Fungi
b) Virus
c) Protozoa
d) Cyanobacteria
19. The nitrogen cycle involves:
a) Only plants and animals
b) Only microorganisms
c) Plants, animals, and microorganisms
d) None of these
20. Which method is most effective in preventing microbial infections?
a) Using antibiotics
b) Sterilizing equipment
c) Drinking boiled water
d) All of the above
21. Which microorganism helps in the production of acetic acid?
a) Yeast
b) Acetobacter
c) Penicillium
d) Rhizobium
22. Cholera spreads through:
a) Air
b) Contaminated water
c) Direct contact
d) Animal bites
23. Edward Jenner is associated with the discovery of the vaccine for:
a) Rabies
b) Smallpox
c) Polio
d) Tuberculosis
24. Which of the following is a preventive measure against malaria?
a) Boiling water
b) Using mosquito nets
c) Wearing masks
d) Sterilizing tools
25. Fungi grow best in:
a) Dry and hot conditions
b) Wet and warm conditions
c) Cold and dry conditions
d) None of these
26. Which microorganism causes tuberculosis?
a) Virus
b) Bacteria
c) Protozoa
d) Fungus
27. The disease anthrax is caused by:
a) Bacillus anthracis
b) Plasmodium vivax
c) Salmonella typhi
d) Vibrio cholerae
28. Viruses can reproduce:
a) Outside living cells
b) Only inside living cells
c) On non-living surfaces
d) Anywhere
29. Which of the following is an example of a parasitic microorganism?
a) Amoeba
b) Plasmodium
c) Yeast
d) Lactobacillus
 30. Aflatoxin is a toxin produced by:
a) Yeast
b) Algae
c) Bacteria
d) Fungi
31. BCG vaccine protects against:
a) Polio
b) Tuberculosis
c) Measles
d) Rabies
32. The microorganism Clostridium botulinum is associated with:
a) Food preservation
b) Food poisoning
c) Antibiotic production
d) Nitrogen fixation
33. Identify the odd one out based on its nature:
a) Penicillium
b) Lactobacillus
c) Rhizobium
d) Plasmodium
34. Deadly diseases like dengue are spread by:
a) Houseflies
b) Mosquitoes
c) Bats
d) Birds
35. Protozoan diseases are transmitted by:
a) Insects
b) Contaminated food and water
c) Direct contact
d) All of the above

Section C: High Order Thinking Skills (HOTS) (15 Questions)

36. Why are antibiotics ineffective against viruses?
a) Viruses do not have metabolic pathways.
b) Viruses reproduce rapidly.
c) Viruses do not have a cell wall.
d) Both a and c
37. In which stage does the malaria parasite multiply in humans?
a) Blood plasma
b) Liver cells
c) Red blood cells
d) Intestines
38. Identify the correct pair:
a) Typhoid – Virus
b) Ringworm – Fungus
c) Cholera – Protozoa
d) Malaria – Bacteria
39. Which microorganism is considered a bioweapon due to its harmful nature?
a) Plasmodium
b) Bacillus anthracis
 c) Salmonella
d) Lactobacillus
40. Which bacteria converts milk into curd?
a) Rhizobium
b) Bacillus
c) Lactobacillus
d) Streptococcus
41. What is the role of decomposers in nature?
a) Fix nitrogen
b) Recycle nutrients
c) Cause diseases
d) All of the above
42. During vaccination, what is introduced into the body?
a) Active pathogens
b) Inactive pathogens
c) Antibiotics
d) Antibodies
43. Which microorganism is used for producing vaccines?
a) Living viruses
b) Dead viruses
c) Fungi
d) None of these
44. Spirulina is an example of:
a) Algae
b) Fungus
c) Bacteria
d) Virus
45. The term "pathogen" refers to:
a) Disease-causing microbes
b) Beneficial microbes
c) Harmless microbes
d) Decomposing microbes
46. Probiotics in food help in:
a) Improving digestion
b) Spreading diseases
c) Preserving food
d) Enhancing flavor
47. Viruses differ from other microorganisms because:
a) They can live independently.
b) They require a host to reproduce.
c) They perform photosynthesis.
d) They are larger than bacteria.
48. Identify the odd one out based on its role:
a) Rhizobium
b) Lactobacillus
c) Bacillus anthracis
d) Yeast
49. The Dengue virus is spread by:
a) Aedes mosquito
b) Anopheles mosquito
 c) Culex mosquito
d) Housefly
50. Microorganisms that live inside the human gut are:
a) Always harmful
b) Only pathogenic
c) Both beneficial and harmful
d) Unimportant