Basic Operations of Automatic Potentiometric Titrator AT-710M/S AN 59-00419-07 Ver.

01

Please take the following procedure to perform routine titration

In this manual, the most frequent titrating operations are illustrated as briefly as possible. When
you have any questions on the operation, please contact a person in charge of titrator.
Some samples to be titrated may contain harmful substances to the human body. Thus, we
recommend that the user should take the following protective measures.
 Wear a disposable white lab coat to prevent liquids from going through the clothes.
 Wear chemical goggles to prevent a sample from splashing into the eyes.
 Wear rubber gloves to keep a sample from contacting you.

Step 1 Turn on the each unit water.

Since the titration nozzle does not need
1 Turn on the main control unit, the measuring calibrating in the next step, let it get back to the
unit and a printer. The order of power-on is nozzle position on the measuring unit.
not necessarily in this order.
Then you will see the boot screen display
Step 4 Calibrate an electrode
followed by the main screen display. (sensor)
The buttons used for performing a titration. Perform calibration of an electrode (sensor) to
[Operator] button (Step 2) be used in conformance with the properties.
Here, only basic aspects of the calibration are
explained.
1 Confirm that “Wait for Titr.” message is
displayed in the message field.
2 Press [Calibration] button.

Reset the instrument

Display “APB manual

operation” screen (Step 5)
Start titration
。 (Step 10)
Display “List of calibration condition”
3 “List of calibration condition” screen will be
screen (Step 4)
displayed.
Display “Function” screen (Step 6) 4 Select a calibration condition for “Ch1/pH” of
“Channel/Unit” with [], [], [] or []
Display “Method list” screen (Step 7)
button.
Step 2 Make measurements by 5 Press [Calib.] button to display “Calibration”
your authorized user name screen.
6 Follow the directions appearing on
1 Press [Operator] button on the top of the “Calibration” screen display.
main screen display.
When carrying out a calibration with standard
Then, “Operator” screen will appear. solutions, wipe off water adhered on the
2 Select your name in the operator list using surface of an electrode (sensor) with tissue
[], [], [] or [] button and press paper or the like.
[OK] button. Your name will be displayed on Following soaking an electrode (sensor) in the
[Operator] button. solution, allow the unit to beat the solution for

Step 3
about five seconds.
Return the titration nozzle 7 Press [Next] button.
to the measuring unit Calibration is initiated and calibration results
will be automatically printed as soon as it is
After the completion of the previous titration, completed with the display of calibration
electrodes (sensors) and titration nozzle values.
remains soaked in a beaker containing pure

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Basic Operations of Automatic Potentiometric Titrator AT-710M/S

Confirm that there appears the message, 5 Set [Purge Mode] to [To Nozzle].
“Calibration results are Success.” on the Setting is the same as in the step 3.
screen.
8 Pressing [Calib. End] button will turn the 6 Set Burette speed to “Slow”.
Setting is the same as in the step 2.
screen display to the main screen.
The reason that Burette speed is set to “Slow”
If the message, “Calibration results are
is to prevent the titrant from being swiftly
Failure.”, is displayed due to some cause
discharged from the nozzle.
such as trouble in an electrode, press [Calib.
End] button again for re-calibration. 7 Take out the titration nozzle immersed in
Step 5 Purge the residue titrant
pure water, followed by putting the tip of it in
another beaker.
Purge the titrant in order to not only uniform the 8 Press [] button.
titrant concentration in a reagent bottle and a
9 Squeeze out the trapped air in the cylinder to
cylinder but purge air bubbles in a cylinder and
a beaker. When the titrant begins to come
a titration nozzle.
out of the tip of the nozzle, press []
1 Press [Burette] button on the main screen button. The piston will stop the operation.
display. Then “APB manual Operation”
10 Take out the titration nozzle from the
screen will be displayed.
electrode holder, followed by inserting the tip
of the titration nozzle in the attached degas
vial.
11 Press [] button with the titration nozzle
held upward.
12 When air bubbles in the titration nozzle are
removed, press [] button. The piston will
stop the operation
13 Pressing [Reset] button allows the piston to
move back to the base position.
2 Enter the number of an operating burette in
the field of [Burette No.]. 14 Attach the titration nozzle to the electrode
Enter the number of piston reciprocation on holder.
the cylinder in the field of [Purge Times]. 15 Press [Exit] button to return to the main
Pressing each button will turn the screen menu screen.
display to “Numeric entry” screen. Then
enter the number and press [OK] button to Step 6 Input information on
confirm the entry, followed by the reagents
disappearance of “Numeric entry” window.
Enter the speed of lifting the piston in a 1 Press [Function] button on the main screen
cylinder in the field of [Burette Speed]. display. “Function 1/2” screen will appear.
Pressing button will turn the screen display
to “Selection” screen. Then select the speed
and press [OK] button to confirm the entry,
followed by the disappearance of “Selection”
window.
Normally, the burette speed is set to ‘Fast’.
In the case of viscous titrant, however, the
speed is lowered by increasing the value
because the faster speed could cause the
suction of air bubbles in dispensing titrant.
3 Set [Purge Mode] to “To Bottle”.
When the mode is set to “To Nozzle”, press
[Purge Mode] button to have the screen 2 Press [Reagent Information] button to
display of selecting ‘Bottle’ or ‘Nozzle’. Then display “Reagent List” screen.
pressing [To Bottle] button will allow the 3 Select the setup item on reagent information
option screen display to disappear and you’ll used for titration with [] or [] button.
find that the purge mode is changed to “To
4 Press [Edit] button to display “Reagent
Bottle”.
Information” screen. Input “Reagent Name”
4 Press [] button. and “Factor” on this screen display.
After repeat purging a set number of times,
the piston in a burette cylinder will stop
automatically.

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Basic Operations of Automatic Potentiometric Titrator AT-710M/S AN 59-00419-07 Ver.01

5 Press [Reagent Name] button to display

Step 7
“Character input” screen.
Confirm measurement
conditions
1 Confirm that “Wait for Titr.” is displayed on
the “Message” field.
If “Wait for Titr.” is not displayed, press
[Reset] button.
2 Press [Method] button to display “Method
List” screen.

6 Inputting a reagent name and then pressing

[OK] button makes the entry confirmed
followed by disappearance of “Character
input” screen.
About key functions:
The button located between [Cancel] button and
[SPACE] button is the cradle-rest button for
changing lowercase (uppercase) words to
uppercase (lowercase) words. When inputting
uppercase (lowercase) words, display “Caps Off.” 3 Select the method to be used with [],
(“Caps On.”) before pressing the corresponding [], [] or [] button.
keys. 4 ［Press [Home] button to display the main
Press [Cancel] button when getting out screen.
“Character input” screen to exit the word input
mode.
Press [Clear] button when deleting words input.
Step 8 Prepare a sample solution
Pressing this button again can recover the This section describes the general procedure to
deletion. take some volume of liquid sample with a
Press [BS] button when deleting a word or pipette. For some liquid samples, sampling is
number just previously input. done on weight basis. Further, for solid or
powder samples, weigh a sample by collecting
Pressing [<<] or [>>] button allows the inputting
on a powder paper or the like. For more
cursor to move to left or right.
information, please ask your manager in charge
7 Press [Factor] button to display “Numeric of operation of this instrument.
input” screen.
1 Attach a pipetter to a 5mL transfer pipette.
2 Take small amount of a sample in the
transfer pipette to rinse the inside of it.
3 Take 5mL of a sample with the pipette and
transfer it to a 200mL beaker.
4 Put a stirrer rotator in the beaker.
5 Pour pure water into the beaker by the
amount for an electrode to have a soak.
6 Rinse the electrodes (sensors) and the
8 When inputting Factor and then pressing titration nozzle, which have been soaked in
[OK] button, your entry is confirmed and the pure water, with pure water, followed by
“Numeric input” window will disappear. wiping off water adhered on them with tissue
About factor: paper or the like.
When using a fresh reagent, input the factor 7 Replace the beaker on the stirrer, where
value indicated on a reagent bottle. When
electrodes or the like have been soaked,
performing factor measurements, just input
the result. For the details of factor with the beaker containing a sample
measurement please see “Perform factor solution.
measurements” on the page 4 in this 8 Soak the electrodes (sensors) and the
manual. titration nozzle in a sample solution.
9 Press [Home] button to return to the main
screen display.

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Basic Operations of Automatic Potentiometric Titrator AT-710M/S

to be rinsed.
Step 9 Input the information on 3 Rinse the electrode (sensor) and the titration
nozzle with pure water.
reagents
4 Wipe off water adhered on the electrode(sensor)
Enter a sample name and a sample ID. and the titration nozzle with tissue paper or the
like.
1 Press [Sample] button on the main screen
to display [Sample Settings] screen. 5 Pour pure water, enough to soak the
electrode (sensor), into another beaker,
followed by immersing the electrode
(sensor) and the titration nozzle in it.
6 Discard the wastewater used for rinsing an
electrode in the designated place.
7 Clean the beaker used.
Practice of factor measurement
The concentration of a titrant or the factor will
change not only with time but when making it up or
2 Press [Sample Name] button to display replacing it with a fresh one. In addition, since a
[Character input] screen. factor will affect the measurement results by titration,
3 Input a sample name and press [OK] button it should be routinely measured.
to confirm the entry of a sample name. Then A factor can be determined with a standard
[Character input] screen will disappear. substance after purging a titrant in a titrator.
With regard to “Character input”, please see
the item 6 of Step 6, “About key functions”. Replacement of Zeolite
4 Press [Sample ID] button to display Periodically—about twice a month—replace the
“Character input” screen. Zeolite, in the Zeolite tube attached to a reagent
(titrant) bottle, with commercial 5A-Zeolite.
5 Input a sample ID and press [OK] button to
confirm the entry of a sample ID. Then Replacement of printer roll paper
[Character input] screen will disappear.
1 Turn off the printer.
2 Detach the printer cover from the printer.
Step 10 Start titration
3 Cut the end of printer roll paper on the square or
1 Allow the screen display to return to “Main” the cross.
by pressing [Home] or [Return] button. 4 Insert the edge of the paper into the loading slot
2 Press [Start] button on the main screen of the printer.
display to allow AT-710M/S to start titration 5 Overset the paper holder outside of the printer,
and plot a titration curve. followed by inserting the printer paper and
3 When a titration ends, the calculation on a securing the core of the roll paper with the paper
set sample size will be performed to show holder.
the measurement results on the screen 6 Turn on the printer.
display and output the results to a printer to
print them out. 7 Press LF key to pull out 5 to 6 cm of printer paper
from the printing unit.
4 Press [OK] button. Then the titration curve
and the titration results will disappear and 8 Attach the printer cover to the printer.
the screen display will return to the main Replacement of ribbon cassette unit
screen.
1 Turn off the printer.
2 Detach the printer cover from the printer and
Step 11 Complete clean-up after the remove a ribbon cassette unit.
end of titration 3 Pick up the slack of the ink ribbon by turning the
knob (on the right of the ribbon cassette) in the
After a titration ends, the following cleaning direction indicated with an arrow.
should be carried out to keep best performance:
4 Insert the ribbon of a ribbon cassette unit in
1 Take out a beaker with a sample from a between the print head and the platen.
stirrer unit.
5 Attach the printer cover to the printer.
2 Place an empty beaker under the electrode