PRODUCT INFORMATION
(±)-11(12)-DiHET
Item No. 51511
Formal Name: (±)11,12-dihydroxy-5Z,8Z,14Z-
eicosatrienoic acid COOH
Synonym: (±)11,12-DiHETrE
MF: C20H34O4
FW: 338.5
Purity: ≥98% HO OH
Supplied as: A solution in ethanol
NOTE: Relative stereochemistry shown in chemical structure
Storage: -20°C
Stability: ≥2 years
Information represents the product specifications. Batch specific analytical results are provided on each certificate of analysis.
Laboratory Procedures
(±)11(12)-DiHET is supplied as a solution in ethanol. To change the solvent, simply evaporate the ethanol
under a gentle stream of nitrogen and immediately add the solvent of choice. Solvents such as DMSO and
dimethyl formamide purged with an inert gas can be used. The solubility of (±)11(12)-DiHET in these solvents
is approximately 50 mg/ml.
Further dilutions of the stock solution into aqueous buffers or isotonic saline should be made prior to
performing biological experiments. Ensure that the residual amount of organic solvent is insignificant, since
organic solvents may have physiological effects at low concentrations. If an organic solvent-free solution of
(±)11(12)-DiHET is needed, it can be prepared by evaporating the ethanol and directly dissolving the neat oil
in aqueous buffers. The solubility of (±)11(12)-DiHET in PBS (pH 7.2) is approximately 1 mg/ml. We do not
recommend storing the aqueous solution for more than one day.
Description
Epoxide hydrolases convert the EETs into vicinal diols, with the concurrent loss of their biological activity.1
(±)11(12)-DiHET relaxes U-46619 (Item No. 16450)-contracted artery rings with approximately 70% of the
magnitude of (±)11(12)-EET (Item No. 50511).2
References
1. Oliw, E.H., Guengerich, F.P., and Oates, J.A. Oxygenation of arachidonic acid by hepatic monooxygenases.
Isolation and metabolism of four epoxide intermediates. J. Biol. Chem. 257(7), 3771-3781 (1982).
2. Fang, X., Kaduce, T.L., Weintraub, N.L., et al. Functional implications of a newly characterized pathway of
11,12-epoxyeicosatrienoic acid metabolism in arterial smooth muscle. Circ. Res. 79(4), 784-793 (1996).
WARNING CAYMAN CHEMICAL
THIS PRODUCT IS FOR RESEARCH ONLY - NOT FOR HUMAN OR VETERINARY DIAGNOSTIC OR THERAPEUTIC USE. 1180 EAST ELLSWORTH RD
SAFETY DATA ANN ARBOR, MI 48108 · USA
This material should be considered hazardous until further information becomes available. Do not ingest, inhale, get in eyes, on skin, or on clothing. Wash thoroughly after
handling. Before use, the user must review the complete Safety Data Sheet, which has been sent via email to your institution. PHONE: [800] 364-9897
WARRANTY AND LIMITATION OF REMEDY [734] 971-3335
Buyer agrees to purchase the material subject to Cayman’s Terms and Conditions. Complete Terms and Conditions including Warranty and Limitation of Liability information
can be found on our website.
FAX: [734] 971-3640
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