EPREUVE de BACTERIOLOGY 3nd semestre : TMS 1 $ HNG
SECTION A
Answer true or false
1- A whole organism is used for lab investigation false
2- A lab request form is not needed from the patient before any investigation is carry out false
3- Specimen for investigation should be collected with minimum contamination from adjacent tissue
or organs true
4- Specimen meant for culture should best be collected before the administration of antibiotic true
5- A speculum is needed for the collection of nigh virginal smears of virgin false
6- Seminal fluid should be collected before abstaining from sex true
7- Specimen should be microscopically examined before lab analysis is done true
8- CSF can be collected by any lab technician false
9- Amies transport medium is effective for specimen collected on swabs true
10- A record of all specimen dispatched from the lab should be kept in the lab true
11- Specimen container having cracks are best used for specimen collect true Specimen containing
highly infections organism should be occasionally be labeled ”high risk” false
12- Culture media should be disinfected before being used false
13- An inoculating wire can be sterilized by flaming true
14- Culture media are usually disinfected by autoclaving true
15- Media in petridishes should be used without examining for contamination false
16- Solid culture media are usually known as broth false
17- Liquid culture media are also known as agar false
18- The surface of the medium in petridishe should always being smooth and free from air bubble
true
19- The zielh nersen staining technic is used to differentiate gram(+) from gram(-) organism false
20- A mordant is a chemical that is used to bring about a staining reaction true
21- Smears on a slide should higher may be dry or dried using gentel heat true
22- Heat fixation will kill microbacterium tuberculosis in sputum false
SECTION B
Choose the correct answer
1- Which of the following specimen should not be considered a “high risk” specimen in the lab
a) Sputum likely to contain M c) Blood specimen that contain HIV
tuberculosis infection
b) Feces specimen that contain V d) Blood specimen that contain
cholerea malaria parasite
2- Specimen for microbiological investigation should be delivered to the lab as soon as possible after
collection in other to
a) Avoid over growth of commensal c) A and b are correct
b) Ensure pathogen are leaving when d) None of the above
the reach the lab
3- A chemical preservative or transport culture media is used to
a) Transport contaminated specimen d) None of the above
to the lab
b) Transport specimen to the lab when
delay in delivery is unavoidable
c) A and b are correct
4- Which of the following is not an exemplar of a transport medium
a) Ami transport medium c) Nutrient agar transport medium
b) Cary Blair transport medium d) A and b are correct
5- In a hospital setting the time limit between collection and delivery of specimen to the lab is
a) 4 – 6 hour c) 5 hours
b) 0 – 2 hours d) None of the above
6- A single swab submitted for multiple request should be
a) Accepted in the lab c) Rejected in the lab
b) Used for analysis d) All of the above are correct
7- Which of these sites is best used for capillary puncture
a) The heel of an adult c) The thumb
b) The heel of an infant d) all of the above are correct
8- In which patient is it not advisable to use a speculum to collect a high vagina specimen
a) A 15 years old of pregnancy human c) A 13 years old sexually active girl
b) A 27 years old female virgin d) all of above is correct
9- In which part of the body is CSF collected
a) The space between the cervical c) The lumbar vertebrate
vertebrate d) none of above is correct
b) The space between the thoracic
vertebrate
10- Sterilization is considered as
a) A helpful degree of removal of c) The complete killing or remove of
pathogenic microorganism all kind of microorganism
b) The partial killing of all find of d) A and b are correct
microorganism
11- Sterilization by filtration can be used
a) To sterilize culture media c) To sterilize serum
b) To sterilize inoculating wire d) none of above is correct
12- Contamination of fluid media is shown by
a) A growth on a media c) a and b are correct
b) Turbidity in a media d) none of above is correct
13- To isolate, identify and study microorganism, they are grown
a) Naturally on the soil
b) On individual
c) On artificial culture media
d) none of above is correct
14- a medium used to grow organism with special growth requirement is
a) basic medium
b) transport medium
c) enrich medium
d) all of above is correct
15- air bubbles can be removed from a medium in a petridishe
a) Centrifuging the medium c) Lightly flaming the surface of the
b) Centrifuging the medium medium
d) none of above is correct
16- An aseptic technic is used when inoculating a culture medium
a) To prevent contamination of culture c) A and b are correct
and specimen d) none of the above is correct
b) To prevent contamination of lab
workers and environment
17- The hanging arop technic can used to
a) Detect bacteria size c) Detect bacteria multicity
b) Detect stability d) A and b are correct
18- Bacteria multiply is
a) The ability of an organism to be c) A and b are correct
moved by another organism d) none of the above is correct
b) The ability of an organism to move
itself in different direction or on a
single direction
19- Stain can be
a) Basic and acidic c) Basic or acid or neutral
b) Neutral and basic d) All of the above is correct
20- Crystal violet is an example of
a) Acid stain c) Basic stain
b) Neutral stain d) All of the above is correct
21- An example of an acid stain is
a) Fuschine c) Methyline blue
b) Eosin d) All of the above is correct
22- Rolling a swab gently on a slide will
a) Avoid damage pus cells c) Kill the organism on the swab
b) Help to make red cell more visible d) None of the above is correct
23- Smear can be fixed by
a) Using normal saline c) Using alcohol
b) Using heat d) b and c are correct
24- Fixation of smears will
a) Preserve microorganism on the slide
b) Prevent smear from being wash off the slide during staining
c) A and b are correct
d) None of the above is correct
25- A gram(-) cell wall is ………… than a gram(+) cell wall
a) Ticker c) Stronger
b) Thinner d) None of the above is correct
26- Cloudiness is a sign that bacteria have grown in a ……. After inoculation and incubation
a) Streak plate c) Gram stain
b) Tube of nutrient broth d) Simple stain
SECTION C
Answer the following questions
1- a- Define sterilization and disinfection
b- List tree method of sterilization you know
2-
a) What is a laboratory culture media
b) List the basic requirement needed by an organism growing in a culture media sulfure
phosphore humidite azote
c) List tree types of culture media you know basi,enrich,transport,differentiate,selective
3- You are expected to collect a urethral smear from a patient suspected of having N gonorrhea
infection. Explain
a) The collection procedure
b) The staining technic you will used
c) What is the mordant used in this technic:lugol iodin
GOOD LUCK
