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The document is a worksheet designed to test knowledge on recombinant DNA (rDNA) technology. It includes questions about the steps and components involved in rDNA processes, such as the use of vectors, enzymes, and the introduction of recombinant DNA into host organisms. Answers can be checked on the provided website link.

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misezehtesham
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690 views1 page

Worksheet

The document is a worksheet designed to test knowledge on recombinant DNA (rDNA) technology. It includes questions about the steps and components involved in rDNA processes, such as the use of vectors, enzymes, and the introduction of recombinant DNA into host organisms. Answers can be checked on the provided website link.

Uploaded by

misezehtesham
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
Available Formats
Download as PDF, TXT or read online on Scribd

Recombinant DNA (rDNA) Technology Worksheet

CHECK, HOW MUCH YOU KNOW ABOUT R-DNA TECHNOLOGY?


[Link]

[Link]
1. The first step in rDNA technology, labelled 1 is ......
2. It is double stranded, self replicating, circular DNA molecule present in bacteria which is widely used
as a gene cloning vector. The structure labelled 2 in the figure is .......
3. These enzymes are called as molecular scissors which is essential for making internal cuts in a DNA
molecule or vector at specific sites. The enzyme used in making cut in the vector (labelled 3) is ......
4. The cut by the enzyme in the vector (labelled 4) creates single stranded unpaired regions of DNA. This
type of cut pattern is called as........
5. 5'GAATTC3' is a restriction site of a widely used restriction enzyme which produces sticky ends. The
enzyme is........
6. In the figure labelled 6, the DNA strand is the ..........
7. This enzyme is called as molecular glue which is used to join two DNA strands by forming
phosphodiester bond. The joining enzyme labelled 7 is ........
8. The vector (plasmid) with foreign gene inserted is called (labelled 8) .....
9. The figure labelled 9 is the process of introducing recombinant vector into a suitable host like
[Link] process is called ......
10. In the figure, 10 a and 10 b are processes that lead to the formation of protein product encoded by
the gene of interest. 10 a and 10 b are ........
Check your answers @ [Link]

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