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Herbal Drug Technology Revision Notes

The document provides quick revision notes on herbal drug technology, focusing on phytochemical screening and thin layer chromatography (TLC). It outlines the purpose of phytochemical screening, differentiates between primary and secondary metabolites, and describes various tests for detecting specific compounds. Additionally, it explains the principles of TLC, including the use of Rf values, silica gel, UV chambers, and the mobile phase.

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Aditya Thakkar
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28 views2 pages

Herbal Drug Technology Revision Notes

The document provides quick revision notes on herbal drug technology, focusing on phytochemical screening and thin layer chromatography (TLC). It outlines the purpose of phytochemical screening, differentiates between primary and secondary metabolites, and describes various tests for detecting specific compounds. Additionally, it explains the principles of TLC, including the use of Rf values, silica gel, UV chambers, and the mobile phase.

Uploaded by

Aditya Thakkar
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
Available Formats
Download as PDF, TXT or read online on Scribd

Herbal Drug Technology - Quick Revision Notes

Phytochemical Screening - Viva Q&A

Q: What is the purpose of phytochemical screening?

A: To detect the presence of bioactive chemical groups responsible for the pharmacological activity of the

plant.

Q: What are primary vs secondary metabolites?

A: Primary metabolites: Essential for plant growth (e.g., sugars, amino acids). Secondary metabolites:

Bioactive compounds (e.g., alkaloids, flavonoids).

Q: Which test is used to detect flavonoids?

A: Shinoda test and Alkaline reagent test.

Q: What indicates the presence of tannins?

A: Blue-black or green coloration with ferric chloride.

Q: What is the principle of the foam test?

A: Saponins reduce surface tension, producing persistent foam when shaken with water.

Thin Layer Chromatography (TLC) - Viva Q&A

Q: What is TLC?

A: TLC is a chromatographic technique used to separate and identify components in a mixture based on

polarity and mobility.

Q: What is an Rf value?
Herbal Drug Technology - Quick Revision Notes

A: The Retention factor (Rf) is the ratio of distance travelled by the compound to that of the solvent front.

Q: Why is silica gel used?

A: Silica gel is polar and porous, making it ideal for separating a wide range of compounds based on polarity.

Q: Why is a UV chamber used?

A: Many phytochemicals are fluorescent or UV-active and become visible under UV light (254 or 366 nm).

Q: What is the purpose of the mobile phase?

A: The mobile phase (solvent system) carries the components up the TLC plate based on solubility and

polarity.

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