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Nutrient Broth

The document describes the preparation and use of Nutrient Broth for cultivating non-fastidious microorganisms in various samples. It outlines the formula, preparation method, storage conditions, and the expected growth results for specific microorganisms. Nutrient Broth is utilized in bacteriological analyses and supports a wide range of microbial growth due to its nutrient content.

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george jh
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167 views1 page

Nutrient Broth

The document describes the preparation and use of Nutrient Broth for cultivating non-fastidious microorganisms in various samples. It outlines the formula, preparation method, storage conditions, and the expected growth results for specific microorganisms. Nutrient Broth is utilized in bacteriological analyses and supports a wide range of microbial growth due to its nutrient content.

Uploaded by

george jh
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
Available Formats
Download as PDF, TXT or read online on Scribd

MICROBIOLOGICAL TEST

NUTRIENT BROTH
For the cultivation of non-fastidious microorganisms The following results were obtained in the performance
in water, feces and other materials of the medium from type cultures after incubation at a
temperature of 35 ± 2°C and observed after 18 - 48
CAT Nº: 21216 hours.

Microorganisms Growth
FORMULA IN g/l
Enterobacter aerogenes ATCC 13048 Good
Gelatin Peptone 5.00 Beef Extract 3.00
Escherichia coli ATCC 25922 Good
Final pH 6.8 ± 0.2 at 25ºC Salmonella typhi ATCC 6539 Good
Staphylococcus epidermidis ATCC 14990 Good
Streptococcus pyogenes ATCC 12344 Moderate
PREPARATION
Suspend 8 grams of the medium in one liter of distilled BIBLIOGRAPHY
water. Mix well and dissolve by heating with frequent Walsbren, Carr, and Dunnette A. J. Clin. Path. 21:884. 1951.
agitation. Boil for one minute until complete dissolution.
American Public Health Association. 1923. Standard methods
Dispense into appropriate containers and sterilize in
of water analysis, 5th ed. American Public Health Association,
autoclave at 121°C for 15 minutes. The prepared
medium should be stored at 2-8°C. The color is amber, Washington, D.C.
slightly opalescent.
STORAGE
The dehydrated medium should be homogeneous, free-
flowing and beige in color. If there are any physical Once opened keep powdered medium closed to avoid
changes, discard the medium. hydration.

USES 25ºC

NUTRIENT BROTH is used for the general cultivation of


2ºC
a wide variety of microorganisms.

A liquid medium, it is produced according to the


formula from APHA and AOAC, and supports the growth
of a great variety of microorganisms that are not very
nutritionally demanding.

This medium is used in accordance with the official


recommended procedures for the bacteriological
analyses of water, milk, dairy products and faeces of
clinical samples, and as a base to prepare media
supplemented with other nutrients. Nutrient Broth is
used in many laboratory procedures as it is or with
added indicators, carbohydrates, organic liquids, salts,
etc.

Gelatin peptone and Beef extract provide nitrogen,


vitamins, minerals and amino acids essential for
growth.

Inoculate medium with the test sample and incubate at


35 ± 2°C for 18 - 48 hours.
Bacteriological agar is the solidifying agent. The
chromogenic mixture contains chromogenic substrates
as Salmon-GAL and X-glucuronide. Coliform enzymes
produced, such as galactosidase and glucuronidase,
cleave these substrates, resulting in the different
coloration of certain bacteria colonies.

DEBEN DIAGNOSTICS LTD www.debendiagnostics.co.uk

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