DEPARTMENT OF MICROBIOLOGY

CHOICE-BASED CREDIT SYSTEM (CBCS)

Learning Outcome-based Curriculum Framework (LOCF)
SYLLABUS

B.Sc. MICROBIOLOGY
2023 - 2024

MADRAS CHRISTIAN COLLEGE

(AUTONOMOUS)
College with Potential for Excellence
Affiliated to University of Madras
Tambaram
Chennai – 600 059
 MADRAS CHRISTIAN COLLEGE

VISION
Madras Christian College aspires to be an Institution of excellence
transforming lives through education with a commitment to service

MISSION
Madras Christian College (MCC) with the inspiration of the love of God
offers to people of all communities’ education of the whole person, which
is congruous with God’s revelation in Christ of the true nature of humanity
and is appropriate to the needs of India and of the world.
 Graduate Attributes
The Madras Christian College defines the philosophy underpinning its academic
programmes and student life experience on campus through the Graduate
Attributes (GA), that describe the knowledge, competencies, values and skills
students imbibe for holistic development and contribution to society. These
attributes encompass characteristics that are transferable beyond the domain of
study into the national and international realm fostered through curricular,
cocurricular and extra-curricular engagements.
GA 1: Intellectual Competencies

● Graduates of MCC have a comprehensive and incisive understanding of

their domain of study as well as the capability for cross-disciplinary
learning.
● They have the ability to apply the knowledge acquired through the
curriculum as well as self-directed learning to a broad spectrum ranging
from analytical thinking to synthesise new knowledge through research.
● Forming independent individual opinions regarding academic cores and
socially relevant issues

GA 2: Professional Ethics

● Graduates of MCC develop ethical and professional behaviour, which will

be demonstrated in their chosen careers and constructive citizenship roles.

●They imbibe intellectual integrity and ethics in scholarly engagement and

develop a spirit of inclusiveness through interactions with people of special
needs and diversity.

GA3: Leadership Qualities

● Graduates of MCC inculcate leadership qualities & attitudes, and team

behaviour along democratic lines through curricular, co-curricular and
extra-curricular activities
● They develop managerial and entrepreneurial skills to ideate and create
new opportunities along with career readiness and capacity to take up
various competitive exams.

GA 4: Holistic Skill Development

● Graduates of MCC develop critical thinking, problem-solving, effective

communication, emotional and social skills
● They develop digital competency to live, learn and serve in society.
GA 5: Cross-Cultural Competencies

● Graduates of MCC imbibe cross-cultural competencies through engaging

with diverse linguistic, ethnic and religious communities providing scope
to understand, accept and appreciate individuals at local, national and
international levels.
● They develop a global perspective through contemporary curriculum,
culture, language and international exchange programmes

GA 6: Service-Oriented Focus

● Graduates of MCC have sensitivity to social concerns and a conviction

toward social justice through a commitment to active social engagement.
● They are endowed with a strong sense of environmental awareness through
the curriculum and campus eco-system.

GA 7: Value-Based Spiritual Development

● Graduates of MCC are rooted in the principles of ethical responsibility and

integrity permeated with Christian values leading to the building of
character.
● They develop virtues such as love, courage, unity, brotherhood, industry
and uprightness.
 Programme Outcomes
Programme Outcomes (POs) of Madras Christian College define the minimum level that
students are expected to do, achieve and/or accomplish in order to graduate from a particular
programme. These Outcomes are a framework to assess the nature of learning activity
experienced within the programme.
POs for Under Graduate Programmes
UG Programmes are designed to have the following outcomes:
On successful completion of the Undergraduate programme, the students will be able to
PO PO Description of PO Mapped with GA
PO 1 Language Skills • Demonstrate oral and GA1, GA4, GA5
written skills to effectively
communicate in English
and Languages of their
choice
• Apply reading and listening
skills to facilitate access to
knowledge resources and
understanding
PO 2 Domain Knowledge • Acquire knowledge of basic GA1, GA3, GA4, GA5
concepts, theories and
processes through study of
core courses in respective
programmes
• Apply and analyze domain
specific knowledge to
emerging areas of academia
and industry
• Assess, adapt and develop
domain specific
transferrable skills to
new/unfamiliar context

PO 3 Interdisciplinary knowledge ● Identify and determine GA1, GA4

relationships across
disciplines
● Acquire and apply
interdisciplinary knowledge
for holistic academic
development

PO 4 Digital Skills ● Acquire computer skills and GA1, GA2, GA3, GA4, GA6
their application relevant to
classroom and self-directed
web-based learning
● Familiarize with and use
domain-related software
resources, computational
skills and digital tools for
data analysis, visualization
and interpretation
 ● Ethically apply digital skills
to creatively communicate a
wide range of ideas and
issues related to academic
experiences

PO 5 Analytical skills ● Develop the ability to think GA1, GA2, GA4, GA6
critically and relate learning
to academic, professional
and real-life problem
solving
● Apply empirical knowledge
and skills to identify and
collect quantitative and
qualitative data to analyze
and formulate evidence-
based suggestions and
solutions

PO 6 Academic writing & ● Formulate and document GA1, GA4, GA5

Presentation skills results obtained in
laboratory, case studies,
project work, field work and
internships
● Effectively communicate
through engaging
presentations using
methodologies appropriate
to the discipline

PO 7 ● Demonstrate transferable GA1, GA2, GA3

Innovation and Creativity capabilities and
intrapreneurial skills that are
relevant to the industry and
other employment
opportunities
● Develop entrepreneurial
skills and generate
intellectual property

PO 8 Social Engagement and ● Demonstrate the ability to GA1, GA2, GA5, GA6, GA7
Responsibility link classroom learning with
social concerns through
service learning and
outreach programmes.
● Enhance positive
personality traits to adapt to
changing circumstances and
demonstrate leadership
qualities as an individual and
a member of cross-cultural
and multi-disciplinary
teams.
● Appreciate environmental
consciousness and
sustainability
● Draw valuable insights from
one’s own spiritual tradition
and that of others for
peaceful coexistence and
general wellbeing
 PROGRAM SPECIFIC OUTCOMES (PSO’s)*

At the time of graduation, they would be able to:

PSO # Statement Mapped with PO#

PSO 1 Relate curriculum with the requirements in the PO1, PO2

discipline of microbiology through imparting of basic
and deeper knowledge.

PSO 2 Apply the microbiological skills acquired in the PO2, PO4, PO5, PO6,
industrial, health, environment and higher education PO7
sectors.

PSO 3 Perceive the significance of microbiology in PO2, PO3

interdisciplinary approaches.

PSO 4 Assume socially responsible roles for the betterment of PO2, PO7, PO8
mankind through microbiology applications.

PSO 5 Choose and create a career in research, industry and PO2, PO7, PO8
bioentrepreneurship.
 Curriculum Template for (B.Sc. Microbiology) (Effective from –2023)
Semester Part Cours Course title Instru Duration Marks Credits
e code ction of exam
hours
per
Cycle ICA ESE Total

I Part I Language 4 3 50 50 100 3

Part II English 4 3 50 50 100 3

Part III

Major Introduction to 6 3 50 50 100 5

Core Microbiology
(Theory)

Major Introduction to 4 6 50 50 100 3

Core Microbiology
(Practical)

Allied Biochemistry I 4 3 50 50 100 4

(Theory)

Allied Biochemistry I 2 3 50 50 100 1

(Practical)

Part IV

a)General Invisible lifeforms 4 3 50 50 100 2

Course and their impact

d)Value Value Education 2 3 50 50 100 1

Education
Total 30 22

II Part I Language 4 3 50 50 100 3

Part II English 4 3 50 50 100 3

Part III

Major Microbial 6 3 50 50 100 5

Core Physiology and
Metabolism (Theory)

Major Microbial 4 6 50 50 100 3

Core Physiology and
Metabolism
(Practical)
 Allied Biochemistry II 4 3 50 50 100 4
(Theory)

Allied Biochemistry II 2 3 50 50 100 1

(Practical)

Part IV

a)General Invisible lifeforms 4 3 50 50 100 2

Course and their impact

d)Value Value Education 2 3 50 50 100 1

Education
Total 30 22

III Part I Language 4 3 50 50 100 3

Part II English 4 3 50 50 100 3

Part III

Major Core Immunology 6 3 50 50 100 5

(Theory)

Major Core Immunology 4 6 50 50 100 3

(Practical)

Allied Bioinstrumentation 4 3 50 50 100 4

(Theory)

Allied Bioinstrumentation 2 3 50 50 100 1

(Practical)

Part IV

b) Interdisciplin Health and Nutrition 4 3 50 50 100 3

ary

c)Environmental Environmental 50 50 100

studies studies - - -

Skill based Personality 2 - - - - -

Development

Total 30 22

IV Part I Language 4 3 50 50 100 3

Part II English 4 3 50 50 100 3

 Part III

Major Core Microbial Genetics 6 3 50 50 100 5

& Molecular Biology
(Theory)

Major Core Microbial Genetics 4 6 50 50 100 3

& Molecular Biology
(Practical)

Allied Biotechnology 4 3 50 50 100 4

(Theory)

Allied Biotechnology 2 3 50 50 100 1

(Practical)

Part IV

b) Interdisciplin Health and Nutrition 50 50 100 -

ary - -

c)Environmental Environmental 4 50 50 100 2

3
studies studies

b) Skill based Personality 2 3 50 50 100 3

Development

Total 30 24

V Part III

Major Core Bacteriology and 5 3 50 50 100 5

Virology (Theory)

Major Core Bacteriology and 3 6 50 50 100 2

Virology (Practical)

Major Core Industrial 5 3 50 50 100 5

Microbiology
(theory)
Major Core Environmental 5 3 50 50 100 5
Microbiology
(Theory)
Major Core Industrial and 6 6 50 50 100 4
Environmental
Microbiology
(Practical)
Part IV
 b) Skill based Computer Training 2 3 50 50 100 3

b) General Bioenergy from 4 3 50 50 100 3

Elective waste resources

Total 30 27

VI Part III

Major Core Parasitology & 6 3 50 50 100 5

Mycology (Theory)

Major Core Parasitology & 4 6 50 50 100 3

Mycology (Practical)

Major Core Food and Dairy 6 3 50 50 100 5

Microbiology
(Theory)
Major Core Pharmaceutical 6 3 50 50 100 5
Microbiology
(Theory)
Major Core Food, Dairy & 4 6 50 50 100 4
Pharmaceutical 4
Microbiology
(Practical)
Total 30 22

Part V Extension activities 1

(NCC/NSS/SFA)

Grand Total 180 140

(Semester I –
Semester VI)
 Semester I – Core Theory
Introduction to Microbiology
Course Code
Credits 5
Hours / Cycle 6
Category Part Core Theory
Semester I
Year of From the academic year 2023-2024
Implementation
1. To learn about fundamental structures of microorganisms and contribution of
pioneers in the field of Microbiology
2. To know about the importance of maintaining aseptic conditions
Course Objectives
3. To carry out media preparation for cultivation of microorganisms
4. To understand the principles of microscopy techniques and their applications
5. To learn about various staining methods for the identification of microorganisms
Bloom’s Taxonomy Levels
PSO
CO # Course Outcome(s) (K1 to K5)
Addressed

On completing the course successfully, the student will be able to

CO 1 Define the concepts of fundamental microbiology PSO1, PSO2 K1

& PSO3

CO 2 Summarize all the aspects of fundamental PSO1, PSO2 K2

microbiology & PSO5

CO 3 Apply the acquired knowledge in all domains of PSO1, PSO2 K3

microbiology. & PSO3

CO 4 Discover various aspects of microbiology based on PSO1, PSO2, K4

fundamental knowledge acquired PSO3 &
PSO5
CO 5 Interpret the significance of microorganisms in PSO1, PSO2, K5
various fields of microbiology PSO3, PSO4
& PSO5

SYLLABUS
UNIT CONTENT HOURS COs BLOOM’S
90 TAXONOMY
LEVEL
I Introduction & Scope of Microbiology. 16 1,2,3, K1,2,3,4,5
Microorganisms and their general characteristics. 4,5
Milestones in the history of Microbiology-
Evolution of microorganisms, Contributions of the
 pioneers – Leewenhoek, Louis Pasteur, Joseph
Lister, Robert Koch, Paul Ehrlich, Edward Jenner,
Alexander Fleming. Recent advancements in
Microbiology.
II Microbial taxonomy - Concepts of taxonomy 18 1,2,3, K1,2,3,4,5
(characterization, classification and nomenclature) 4,5
and systematics; classification of microorganisms -
Three kingdom, Five kingdom concept and Eight
kingdom concept. Outline of Bergey’s system of
classification. Brief outline on molecular based
methods of classification.

III Prokaryotic cell structure and functions - Cell wall, 20 1,2,3, K1,2,3,4,5
Cell membrane, Components external to cell wall- 4,5
Capsule, Slime layer, S layer, Flagella, Pili,
Fimbriae. Cytoplasmic matrix-Inclusion bodies,
magnetosomes, ribosomes, gas vesicles. Bacterial
endospores and their formation. Structure of
eukaryotic microorganisms and their cell organelles.
Difference between prokaryotes and eukaryotes.
IV Microscopy – working principle and applications of 18 1,2,3, K1,2,3,4,5
simple, compound, dark field, phase contrast, 4,5
Fluorescence & Electron microscopy – SEM and
TEM. Staining - Dyes and stains: Types. Simple and
differential staining. Special staining (Acid Fast,
Capsule, Spores & Metachromatic granules).
Motility test - Hanging drop technique.
V Culture media – types, cultivation of anaerobes and 18 1,2,3, K1,2,3,4,5
Pure culture techniques – serial dilution, spread, pour 4,5
and streak plate. Maintenance and preservation of
cultures. Sterilization – physical and chemical
methods. Sterility control of autoclave and hot air
oven (chemical and biological indicators). QC of
other/chemical methods. Newer sterilization –
Plasma sterilization

Prescribed Books/Textbooks
1.Pelczar M.J., Chan E.C.S. and Kreig. N.R. (2007). Microbiology 7th edn. McGraw-Hill New York
2.Lansing M. Prescott., John. P. Harley. Donald A. Klein (2013). Microbiology, 9 th Edition, Mc Graw
Hill Inc. New York.
3.Cappucino J and Sherman N. (2011). Microbiology: A Laboratory Manual. 9th edition. Pearson
Education Limited.
4.Tortora, G.J., Funke B.R. and Case, C.L. (2013). Microbiology-An Introduction, 11th edn. Benjamin
Cummings.
References
1.Nester E., Anderson D., Roberts C. E., and Nester M. (2006). Microbiology-A Human Perspective,
5th edn. McGraw Hill Publications.
2.Wheelis M. (2007). Principles of Modern Microbiology, 1st edn. Jones and Bartlett Publishers, Inc.
3.Gerard J. Tortora, Berdell R. Funke, Christine L. Case, Derek Weber (2014). Microbiology: An
Introduction, 12th Edition.
4.Stanier R.Y, Ingraham J.L., Wheelis M.L., and Painter R.R. (2010). General Microbiology, 5 th edn.
MacMillan Press Ltd.
5.Prescott L.M, Harley J.P and Klein D.A. (2013). Microbiology 9th edn, McGraw-Hill
Suggested Reading
1.R.C. Dubey and O. K. Maheswari S. (2003). A textbook of microbiology
2. Madigan Michael T. Martinko John M. Bender Kelly S. (2017) - Brock Biology of Microorganisms
Web Resources
1.http://www.mmc.gov.bd/downloadable%20file/Introduction&%20history%20of%20mihttp://www.
mmc.gov.bd/downloadable file/Introduction%26 history of microbiology for
fb.pdf1crobiology%20for%20fb.pdf1.
2.http://light.ece.illinois.edu/ECE460/PDF/Chap%20XVIII%20-%20Microscopy_a.pdf
3. https://microbeonline.com/types-of-bacteriological-culture-medium/
4.https://www.thepharmaeducation.com/2020/07/sterilization-techniques-different-methodsofsterilization.html
5. https://open.oregonstate.education/generalmicrobiology/chapter/microbial-growth

Course Articulation Matrix

Programme Outcomes
Programme Specific Outcomes
Cognitive Level
Course
Outcomes
PO1 PO2 PO3 PO4 PO5 PO6 PO7 PO8 PSO1 PSO2 PSO3 PSO4 PSO5
K1
CO 1 2 3 3 2 3 3 3 - 3 3 3 - -

K2
CO 2 2 3 3 2 3 3 3 3 3 3 - - 3

K3
CO 3 2 3 3 2 3 3 3 - 3 3 3 - -

K4
CO 4 2 3 3 2 3 3 3 3 3 3 3 - 3

K5
CO 5 2 3 3 2 3 3 3 3 3 3 3 3 3

Wt. Avg. 2 3 3 2 3 3 3 3 3 3 3 3 3

Wt. Avg. 2.8 3

Overall Mapping of the Course With PO 2.8

With PSO 3
 Semester I – Core Practical
Introduction to Microbiology
Course Code
Credits 3
Hours / Cycle 4
Category Part Core Practical
Semester I
From the academic year 2023 -2024
Year of
Implementation

1.To develop skills in handling laboratory instruments and microscopes.

2.To know about various staining techniques in identifying microorganisms
Course Objectives 3.To identify the structure of algae and fungi by appropriate methods
4.To learn about the preparation of media and standard culturing methods.
5.To demonstrate the microbial load in the given sample.
Bloom’s Taxonomy Levels
PSO
CO # Course Outcome(s) (K1 to K5)
Addressed

On completing the course successfully, the student will be able to

CO 1 List and recall the operation of microbiology labPSO1, PSO2, K1

equipment, microscopes and staining and culture PSO4 &
techniques. PSO5
CO 2 Explain the materials, equipment and relate their use
PSO1, PSO2, K2
in practical microbiology. PSO4 &
PSO5
CO 3 Apply the techniques needed for performing basic PSO1, PSO2, K3
microbiology practical. PSO3 &
PSO5
CO 4 Examine and analyse the results obtained through PSO1, PSO2, K4
basic microbiology practical PSO4 &
PSO5
CO 5 Evaluate and explain the methods of basic PSO1, PSO2, K5
microbiological techniques PSO4 &
PSO5

SYLLABUS
UNIT CONTENT HOURS COs BLOOM’S
60 TAXONOMY
LEVEL
 1 Biosafety and biohazard guidelines in microbiology 6 1,2,3, K1,2,3,4,5
laboratory. 4,5
Operation of laboratory equipment – LAF, incubator
and colony counter.
Principle & methods – Moist heat and dry heat
sterilization.
Validation of sterilization procedures.

2 Microscopy: Handling and operation of 15 1,2,3, K1,2,3,4,5

microscopes – Bright-field and Dark- 4,5
field Microscopes.

3 Pure culture techniques – streak, pour 12 1,2,3, K1,2,3,4,5

and spread plate. 4,5
Quantitative estimation of
microorganisms – total and viable
counts.

4 Staining technique - smear preparation, Simple 12 1,2,3, K1,2,3,4,5

staining, Gram staining, Negative staining, Acid fast 4,5
Staining, Capsule & Endospore staining.
Microscopic observation of fungal morphology -
Lactophenol Cotton Blue staining.
Demonstration of motility of
microorganisms by Hanging drop and
Semisolid agar
Direct examination of Algae by wet mount
5 Preparation of nutrient agar slants and culturing of 15 1,2,3, K1,2,3,4,5
bacteria; Stab culture. 4,5

Prescribed Books/Textbooks
1. R.C. Dubey, D.K. Maheshwari and S. Chand (2012). Practical Microbiology. Publisher: S. Chand
Publications
2. Aneja K.R. (2007). Experiments in Microbiology, Plant Pathology and Biotechnology (4 th edition).
New Age International.
3. Marimuthu R. (2008). Microscopy and Microtechniques. MJP Publishers.
References
1.Amita Jain, Vimala Venkatesh, Jyotsna Agarwal. (2019). Microbiology Practical Manual, First
edition, Elsevier -Saunders, Mosby, Churchill.
2.R. Saravanan, D. Dhachinamoorthi.A and CH.MM. Prasada Rao. (2019). Handbook of Practical
Microbiology, Publisher: Lambert Academic Publishing.
3.Emanuel Goldman and Lorrence H Green. (2021). Practical Handbook of Microbiology, 4 th edition,
Publisher: CRC Press.
Suggested Reading
1.Gunasekaran. (2018). Laboratory Manual in Microbiology, Second edition, New Age International
(P) Ltd Publishers.
2. S. Rajan (2020) Essential of Microbiology, CBS Publishers And Distributors Pvt Ltd
Web Resources
1.https://bio.libretexts.org/Learning_Objects/Laboratory_Experiments/Microbiology_Labs/Bo
ok%3A_General_Microbiology_Lab_Manual_(Pakpour_and_Horgan)
2. https://microbiologysociety.org/publication/education-outreach-
resources/basicpracticalmicrobiology-a-manual.html
3. https://www.researchgate.net/publication/257380059_Laboratory_Manual_in_General_Microbiolog
y_For_Undergraduate_Students_Short_Versio

Course Articulation Matrix

Programme Outcomes
Programme Specific Outcomes
Cognitive Level
Course
Outcomes
PO1 PO2 PO3 PO4 PO5 PO6 PO7 PO8 PSO1 PSO2 PSO3 PSO4 PSO5
K1
CO 1 2 3 - 2 2 3 2 3 3 3 - 3 3

K2
CO 2 2 3 - 3 2 3 3 3 3 3 - 3 3

K3
CO 3 2 3 3 2 2 3 2 3 3 3 2 - 3

K4
CO 4 2 3 - 2 2 3 2 3 3 3 - 3 3

K5
CO 5 2 3 - 2 2 3 2 3 3 3 - 3 3

Wt. Avg. 2 3 3 2.2 2 3 2 3 3 3 2 3 3

Wt. Avg. 2.5 2.8

Overall Mapping of the Course With PO: 2.5

With PSO: 2.8
 Semester I - Allied Theory
Biochemistry –I
Course Code
Credits 4
Hours / Cycle 4
Category Part Allied Theory
Semester I
From the academic year 2023 -2024
Year of
Implementation

1.To learn the structure and importance of carbohydrates

2. To know the protein classification and their structural organization
Course Objectives 3. To gain knowledge on different types of lipids and their functions
4.To understand the structure and types of DNA and RNA
5.To know the importance of vitamins and minerals in normal body function.
Bloom’s Taxonomy Levels
PSO
CO # Course Outcome(s) (K1 to K5)
Addressed

On completing the course successfully, the student will be able to

CO 1 List the biomolecules of a cell and their significance PSO1 K1

PSO3, PSO4
PSO5
CO 2 Classify the biomolecules in detail PSO1, K2
PSO3, PSO5

CO 3 Identify the biological importance of biomolecules PSO1 K3

PSO3, PSO4
PSO5
CO 4 Analyse the structure and properties of biomolecules PSO1 K4
PSO3, PSO4
PSO5
CO 5 Interpret the types, importance and deficiency PSO1 K5
disorders of biomolecules PSO3, PSO4
PSO5

SYLLABUS
UNIT CONTENT HOURS COs BLOOM’S
90 TAXONOM
Y
LEVEL
 I Introduction to biomolecules. 12 1,2,3,4, K1,2,3,4,5
Carbohydrates – definition, classification and 5
importance. monosaccharides, disaccharides
(maltose, lactose and sucrose), polysaccharides –
homo and heteropolysaccharides - structure,
occurrence and properties.

II Amino acids-classification based on functional 12 1,2,3,4, K1,2,3,4,5

group, essential and non- 5
essential amino acid, peptide bond. Proteins –
classification – shape, solubility and composition
and biological function. Properties of proteins –
ampholytes, isoelectric point, salting in and salting
out, denaturation. Organization of protein structure
– primary, secondary, tertiary and quaternary
structure

III Classification and functions of lipids. Fatty acids 10 1,2,3,4, K1,2,3,4,5

(saturated and unsaturated). Properties - 5
saponification, rancidity. Functions of
phospholipids. cholesterol – Structure and
biological importance.

IV Nucleic acids - purines and pyrimidine bases, 9 1,2,3,4, K1,2,3,4,5

nucleosides, nucleotides, polynucleotides. DNA- 5
structure and types.
RNA- structure and types.

V Vitamins definition, classification -Fat soluble 17 1,2,3,4, K1,2,3,4,5

vitamins (A, D, E, K) and Water-soluble vitamins 5
(B1, B2, B3, B6, B12 and C): sources, requirements
functions and deficiency disorder. Macro minerals-
calcium and phosphorus: Micro minerals- iron,
iodine, zinc. sources, functions, and effects of
deficiency.

Prescribed Books/Textbooks
1.Victor W. Rodwell. (2018). Harper’s Illustrated Biochemistry. 31st Edition. McGraw-Hill.
2.Nelson, DL and Cox MM., Lehninger. (2021). Principles of Biochemistry, 8th ed. WH Freeman.

References
1.David E Metzler: Biochemistry (2003). The Chemical reactions of Living Cells Vol. 1. 2 nd Edition,
Elsevier Academic Press.
2.Berg JM, Tymoczko JL and Stryer L. (2019). Biochemistry, 9th Edition, WH Freeman and Company.
3.Voet, D.J and J.G. Voet and C.W. Pratt. (2010). “Principles of Biochemistry” 3rd Edition, John Wiley &
Sons Inc.
4.Plummer DT (2017). An Introduction to Practical Biochemistry. Tata McGraw-Hill Publishing
Company Limited.
 Suggested Reading
1.Jain, J.L., Jain, S. and Jain, N. (2016). Fundamentals of Biochemistry, S. Chand and Company Ltd.
2.Satyanarayan, U.Chakrapani. (2021). Biochemistry, 6th Ed. Elsevier

Web Resources
1. https://microbenotes.com/carbohydrates-structure-properties-classification-andfunctions/
2. https://bio.libretexts.org/Bookshelves/Microbiology/Microbiology_(OpenStax)/07%
3A_Microbial_Biochemistry/7.03%3A_Lipids
3. https://med.libretexts.org/Courses/Allan_Hancock_College/Introduction_to_Nutritio
n_Science_(Bisson_et._al)/10%3A_Vitamins_and_Minerals_Overview/10.03%3A_
Sources_of_Vitamins_and_Minerals

Course Articulation Matrix

Programme Outcomes
Programme Specific Outcomes
Cognitive Level
Course
Outcomes
PO1 PO2 PO3 PO4 PO5 PO6 PO7 PO8 PSO1 PSO2 PSO3 PSO4 PSO5
K1
CO 1 2 3 3 - - - 3 3 3 - 3 2 2

K2
CO 2 2 3 3 - - - 3 3 3 - 3 - 2

K3
CO 3 2 3 3 - - - 3 3 3 - 3 2 2

K4
CO 4 2 3 3 - - - 3 3 3 - 3 3 3

K5
CO 5 2 3 3 - - - 3 3 3 - 3 3 3

Wt. Avg. 2 3 3 - - - 3 3 3 - 3 2.5 2.4

Wt. Avg. 2.8 2.7

Overall Mapping of the Course With PO 2.8

With PSO 2.7
 Semester I – Allied Practical
Biochemistry –I
Course Code
Credits 1
Hours / Cycle 2
Category Part Allied Practical
Semester I
From the academic year 2023 -2024
Year of
Implementation

1.To identify the carbohydrates by qualitative analysis

2.To differentiate monosaccharides, disaccharides and
polysaccharides by microscopy.
Course Objectives
3.To identify the different amino acids
4.To understand the properties of carbohydrates and amino acids
5.To develop practical skills in biochemical preparations of starch and casein.
Bloom’s Taxonomy
PSO Levels
CO # Course Outcome(s)
Addressed (K1 to K5)

On completing the course successfully, the student will be able to

CO 1 Recall and list the different types of carbohydrates PSO1, K1

and amino acids, and biochemical preparations PSO3

CO 2 Relate the carbohydrates, amino acids PSO1, K2

and casein based on the properties PSO3 PSO5

CO 3 Identify the carbohydrates, amino acids and casein PSO1, K3

by qualitative analysis PSO3 PSO4
PSO5
CO 4 Examine the different carbohydrates, amino acids PSO1, K4
and casein by suitable methods PSO3 PSO4
PSO5
CO 5 Discuss the results with confirmatory tests PSO1, K5
PSO3 PSO4
PSO5

SYLLABUS
UNIT CONTENT HOURS COs BLOOM’S
30 TAXONOMY
LEVEL
 I Qualitative analysis of 8 1,2,3,4,5 K1,2,3,4,5
Monosaccharides: xylose, glucose,
fructose, galactose,
II Qualitative analysis of 6 1,2,3,4,5 K1,2,3,4,5
Disaccharides: lactose, maltose and
sucrose
III Qualitative analysis of 2 1,2,3,4,5 K1,2,3,4,5
polysaccharides: starch

IV Qualitative analysis of 12 1,2,3,4,5 K1,2,3,4,5

amino acids: Arginine,
Cystine, Tryptophan,
Tyrosine and
Histidine
V Biochemical Preparation: 2 1,2,3,4,5 K1,2,3,4,5
Preparation of starch from
potatoes.
Preparation of casein and
lactalbumin from milk
Prescribed Books/Textbooks
1.Plummer DT. An Introduction to Practical Biochemistry. (2017). Tata McGraw-Hill
Publishing Company Limited
2.Randhir Singh and S. K. Sawhney. (2014). Introductory Practical Biochemistry,10th reprint
Narosa Publishers
References
1.DM Vasudevan Sudhir Kumar Das (2020) Practical Textbook of Biochemistry for
Medical Students Jaypee Brothers Medical Publishers (P) Ltd
2.Berg JM, Tymoczko JL and Stryer L. (2019). Biochemistry, 9th Edition, WH Freeman and
Company
3.Sawhney (2000) Introductory practical biochemistry, Alpha science International limited
4.Punit Puri (2021). Techniques in biochemistry. Walnut Publication
Suggested Reading
1.GG Kaushik Neha Sharma Sabira Dabeer, Ruchi Jindal (2020) Practical Manual of
Biochemistry, CBS Publishers.
2.Geetha Damodaran (2016). Practical Biochemistry Jaypee Brothers Medical Publishers (P) Ltd

Web Resources
1.https://biologyreader.com/qualitative-analysis-of-amino-acids.html
2.https://link.springer.com/chapter/10.1007/978-3-319-45776-5_19
3.https://biologyreader.com/qualitative-analysis-of-carbohydrate.html
 Course Articulation Matrix

Programme Outcomes
Programme Specific Outcomes
Cognitive Level
Course
Outcomes
PO1 PO2 PO3 PO4 PO5 PO6 PO7 PO8 PSO1 PSO2 PSO3 PSO4 PSO5
K1
CO 1 3 3 3 - - - - - 3 - 3 - -

K2
CO 2 3 3 3 - - - 2 2 3 - 3 - 2

K3
CO 3 3 3 3 - - - 2 2 3 - 3 3 2

K4
CO 4 3 3 3 - - - 2 2 3 - 3 3 2

K5
CO 5 2 3 3 - - - 2 2 3 - 3 3 2

Wt. Avg. 2.8 3 3 - - - 2 2 3 - 3 3 2

Wt. Avg. 2.6 2.8

Overall Mapping of the Course With PO 2.6

With PSO 2.8
 Semester I & II – General Course
Invisible Lifeforms and their impacts
(For Non-Microbiology Students)
Course Code

Credits 2
Hours / Cycle 4
Category Part Core Theory
Semester I & II
Year of From the academic year 2023-2024 onwards
Implementation
1. To study about the various groups of microorganisms and their significance.
2. To understand the applications of microorganisms in the food industry.
Course Objectives 3. To know about Communicable diseases.
4. To comprehend the use of microbes in waste disposal.
5. To understand the role of microbes in soil fertility.

Bloom’s Taxonomy Levels

PSO
CO # Course Outcome(s) (K1 to K5)
Addressed

On completing the course successfully, the student will be able to

CO 1 Relate various groups of microbes, their control PSO1, PSO3, K1

measures, and its significance in day-to-day life. PSO4

CO 2 Explain the basics of microscopy, and applications PSO1, PSO3, K2

of microorganisms in different fields of PSO4
microbiology as well as its disease-causing ability.

CO 3 Identify the concepts of disease control and PSO1, PSO3, K3

importance of microbes in medical, food, PSO4, PSO5
agriculture and environmental sectors.
CO 4 Discover knowledge about various groups of PSO1, PSO3, K4
microorganisms, their preventive measures, and PSO4
their role in different fields.
CO 5 Recommend healthy living and disease prevention PSO1, PSO3, K5
methods with the knowledge of microbes in various PSO4, PSO5.
disciplines.
 SYLLABUS
UNIT CONTENT HOURS COs BLOOM’S
60 TAXONOMY
LEVEL
I Introduction to Microbiology. Groups of 10 1,2,3,4, K1,2,3,4,5
microorganisms – Bacteria, Fungi, Virus, Algae and 5
Protozoa. Microorganisms and its habitat.
Microbiology and society. Significance of
Microscopes.

II Disinfection – Sanitizers and antiseptics. Safety 12 1,2,3,4 K1,2,3,4,5

practices - PPE, classification of biomedical waste ,5

and its disposal methods. Outline of Antibiotic

therapy and vaccines. Immunization schedule.

III Role of microbes in fermented foods - Pasteurization 10 1,2,3,4, K1,2,3,4,5

- butter and cheese, probiotics - curd and yoghurt, 5
fermented foods - idli, bread, pickle, alcoholic
beverages-wine.

IV Role of microbes in Agriculture and Environment - 14 1,2,3,4, K1,2,3,4,5

as biofertilizers, biopesticides and its advantages. 5
Methods of waste disposal - Composting and sewage
treatment.

V Communicable diseases: Water and food borne 14 1,2,3,4, K1,2,3,4,5

diseases- Typhoid, Cholera, Polio, and Amoebiasis. 5
Bioaerosols and air borne diseases - Tuberculosis,
COVID -19 and common cold. Insect borne -
Malaria. Sexually transmitted diseases – AIDS and
Hepatitis B.

Prescribed books/Text Books

1. Dubey R. C. & Maheshwari D. K. (2010). A Text Book of Microbiology. S. Chand and company
Pvt. Ltd.
2. Cappucino J and Sherman N. (2011). Microbiology: A Laboratory Manual. 9th edition. Pearson
Education Limited.
3. Gerard J. Tortora, Berdell R. Funke, Christine L. Case, Derek Weber (2014). Microbiology: An
Introduction, 12th Edition. Pearson publisher.
4. Powar C.H. Daginawala H.F (2012). General Microbiology.Vol-I & II, Himalaya Publishing House.
 References
1. Lansing M. Prescott., John. P. Harley. Donald A. Klein (2013). Microbiology, 9 th Edition, Mc Graw
Hill Inc. New York.
2. Stanier RY, Ingraham JL, Wheelis ML, and Painter PR. (2005). General Microbiology. 5th edition.
McMillan.
3. Pepper I.L., Gerba C.P. & Gentry T.J. (2014). Environmental Microbiology (3rd edition). Academic
Press, Elsevier.
4. Tinsley S., & Pillai I. (2012). Environmental Management Systems – Understanding Organizational
Drivers and Barriers. Taylor & Francis group.
Suggested Reading
1. Willey J., Sherwood L., and Woolverton C. J., (2017). Prescott’s Microbiology. 10 th Edition.,
McGraw-Hill International edition.
2. Jeffrey C and Pommerville. (2010). Alcamo’s Fundamentals of Microbiology.9th Edition. Jones
&Bartlett Publisher.
3. Subbarao N.S. (2017). Bio-fertilizers in Agriculture and Forestry. 4th Edition. Med tech publisher.
4. Dubey, R. C. (2008). A Textbook of Biotechnology. S. Chand & Co., New Delhi.
5. Ananthanarayanan, R. and JayaramPanicker C.K. (2020) Text book of Microbiology. Orient
Longman, Hyderabad.
Web Resources
1. https://nptel.ac.in/courses/126105016
2. http://www.biologydiscussion.com/micro-biology/sterilisation-and-disinfection-methods-andprinciples-
microbiology/24403.
3. https://nptel.ac.in/courses/126103017
4. https://www.thepharmaeducation.com/2020/07/sterilization-techniques-different-methods-
ofsterilization.html
5. https://www.classcentral.com/course/swayam-food-microbiology-and-food-safety-17609

Course Articulation Matrix

Programme Outcomes
Programme Specific Outcomes
Cognitive Level
Course
Outcomes
PO1 PO2 PO3 PO4 PO5 PO6 PO7 PO8 PSO1 PSO2 PSO3 PSO4 PSO5
K1
CO 1 2 3 3 - - - 3 3 3 - 2 3 -

K2
CO 2 2 3 3 - - - 2 3 3 - 3 3 -

K3
CO 3 2 3 3 - - - 3 3 3 - 2 3 3

K4
CO 4 2 3 3 - - - 3 3 3 - 3 3 -

K5
CO 5 2 3 3 - - - 3 3 3 - 2 3 -

Wt. Avg. 2 3 3 - - - 2.8 3 3 - 2.4 3 3

Wt. Avg. 2.8 2.9

Overall Mapping of the Course With PO 2.8

With PSO 2.9
 Semester II– Core Theory
Microbial Physiology & Metabolism
Course Code

Credits 5
Hours / Cycle 6
Category Part Core Theory
Semester II
Year of From the academic year 2023-2024 onwards
Implementation
1. Understand the nutritional requirements of microorganisms.
2. Recall the basics of microbial growth.
Course Objectives 3. Know about the aerobic and anaerobic metabolic pathways.
4. Determine the energy yielding mechanisms.
5. Learn about the methods of microbial reproduction.
Bloom’s Taxonomy
PSO Levels
CO # Course Outcome(s)
Addressed (K1 to K5)

On completing the course successfully, the student will be able to

CO 1 Relate the microbial growth, metabolism and of PSO1, PSO2 K1
reproduction with their nutritional PSO3& PSO5
requirements.
CO 2 Demonstrate the mechanisms of nutrient PSO1, PSO2, K2
uptake, growth kinetics and metabolic PSO3 &
pathways by microorganisms. PSO5
CO 3 Experiment with the physiology of PSO1, PSO2, K3
microorganisms. PSO3 &
PSO5
CO 4 Discover the principles of microbial physiology PSO1, PSO2, K4
and metabolism. PSO3 &
PSO5
CO 5 Determine the significance of the mechanisms PSO1, K5
involved in microbial physiology PSO2&
PSO3

SYLLABUS
UNIT CONTENT HOURS COs BLOOM’S
90 TAXONOMY
LEVEL
 I Microbial Nutrition- Nutritional requirements – 13 1,2,3,4,5 K1,2,3,4,5
Macro, micronutrients and trace elements. Nutritional
types. Nutrient uptake mechanisms - passive
diffusion, facilitated diffusion, active transport, group
translocation, Iron uptake.
II Physiology of microbial growth - generation time, 20 1,2,3,4,5 K1,2,3,4,5
Bacterial growth curve and measurement of growth,
growth kinetics. Batch, Fed-batch, continuous,
semicontinuous and synchronous cultures: steady
state, chemostat and Turbidostat. Factors affecting
microbial growth.

III Microbial metabolism - Concept of Metabolism: 20 1,2,3,4,5 K1,2,3,4,5

Anabolism, Catabolism. Central catabolic pathways
– glycolysis, glyoxylate pathway, hexose
monophosphate pathway, Entner
Doudoroff pathway, tricarboxylic acid cycle – electron
transport system, bioenergetics.
IV Physiology of Fermentation- Homolactic, 20 1,2,3,4,5 K1,2,3,4,5
heterolactic and mixed acid. Photosynthesis:
oxygenic and anoxygenic photosynthesis in
prokaryotes, photophosphorylation; Calvin cycle.
V Bacterial reproduction - Binary fission, Budding, 17 1,2,3,4,5 K1,2,3,4,5
Reproduction through conidia, cyst formation,
endospore formation. Asexual and sexual
reproduction – Fungi, Microalgae and protozoa.
Eukaryotic cell division
Prescribed books/Text Books:
1.Schlegal, H.G. (2003). General Microbiology.7th Edition, Press syndicate of the University of
Cambridge.
2.Rajapandian K. (2010). Microbial Physiology. PBS Book Enterprises India.
3.Meena Kumari. S. (2006) Microbial Physiology, 1st Edition MJP Publishers.
4.Dubey R.C. and Maheswari, S. (2003). A textbook of Microbiology, New Delhi: S. Chand & Co.
5.S. Ram Reddy, S.M. Reddy (2008). Microbial Physiology. Anmol Publications Pvt Ltd.
References
1.Robert K. Poole (2004). Advances in Microbial Physiology, Elsevier Academic Press, New York,
Volume 49.
2.Kim B.H., Gadd G.M. (2008). Bacterial Physiology and Metabolism. Cambridge University Press,
Cambridge.
3.Daniel R. Caldwell. (2000). Microbial Physiology & Metabolism, 2nd edition. Star publication.
4.Bhanu Shrivastava. (2011). Microbial Physiology and Metabolism: Study of Microbial Physiology and
Metabolism. Lambert academic Publication.

Suggested Reading
1. Moat, A.G and J.W Foaster (2002). Microbial Physiology, 4th edition. Wiley – LISS, A John Wiley
& Sons. Inc. Publications.
2. S. Ram Reddy, S.M. Reddy (2008). Microbial Physiology. Anmol Publications Pvt Ltd.
Web Resources:
1. https://onlinecourses.swayam2.ac.in/cec21_bt17/preview
2.https://www.studocu.com/in/document/amity-university/microbiology/microbial-growth-
andcontrol/4680389
3. https://www.studocu.com/in/document/amity-university/microbiology/microbial-nutrition/4680387

Course Articulation Matrix

Programme Outcomes
Programme Specific Outcomes
Cognitive Level
Course
Outcomes
PO1 PO2 PO3 PO4 PO5 PO6 PO7 PO8 PSO1 PSO2 PSO3 PSO4 PSO5
K1
CO 1 2 3 2 - 3 2 2 - 3 3 - - 3

K2
CO 2 2 3 2 2 3 2 3 3 3 3 3 - 2

K3
CO 3 2 3 3 2 3 2 3 3 3 3 3 2

K4
CO 4 2 3 2 2 3 2 3 - 3 3 3 - 3

K5
CO 5 2 3 2 2 3 2 3 - 3 3 3 - -

Wt. Avg. 2 3 2.2 2 3 2 2.8 3 3 3 3 - 2.5

Wt. Avg. 2.5 2.9

Overall Mapping of the Course With PO 2.5

With PSO 2.9
 Semester II – Core Practical
Microbial Physiology and Metabolism
Course Code

Credits 3
Hours / Cycle 4
Category Part Core Practical
Semester II
Year of From the academic year 2023-2024
Implementation

1. To demonstrate the measurements of bacterial growth.

2. Learn about the factors affecting microbial growth.
Course Objectives
3. Gain knowledge on cultivation of anaerobes.
4. Provide insights on microbial fermentation.
5. Identify different reproductive structures of microorganisms.

Bloom’s
PSO Taxonomy Levels
CO # Course Outcome(s)
Addressed (K1 to K5)

On completing the course successfully, the student will be able to

CO 1 Recall and list the principles and procedures of each PSO1, PSO2 K1
experiment pertaining to microbial physiology and PSO3
their metabolism
CO 2 Summarize and relate the apparatus and other PSO1, PSO2 K2
materials of use in microbial physiology practical PSO3
CO 3 Apply and make use of appropriate techniques in PSO1, PSO2 K3
microbial physiology practical PSO3
CO 4 Analyse the significance of each of the methods PSO1, PSO2 K4
performed PSO3
CO 5 Evaluate the techniques and their outcomes used in PSO1, PSO2 K5
microbial physiology practical PSO3

SYLLABUS
UNIT CONTENT HOURS COs BLOOM’S
60 TAXONOMY
LEVEL
 I Bacterial growth curve by turbidometry method. 17 1,2,3,4, K1,2,3,4,5
Bacterial growth measurement by direct and viable 5
count method.
Cultivation of anaerobic bacteria
II Growth of the bacteria at different pH 12 1,2,3,4, K1,2,3,4,5
Effect of different temperatures on microbial growth 5
III Carbohydrate fermentation test 6 1,2,3,4, K1,2,3,4,5
5
IV Isolation of Pigment producing bacteria 12 1,2,3,4, K1,2,3,4,5
Estimation of chlorophylls from Cyanobacteria 5
V Microscopic identification of asexual, sexual spores 13 1,2,3,4, K1,2,3,4,5
of algae and fungi 5
Prescribed Books/Textbooks
1. Sundararaj T (2005). Microbiology Lab Manual,1st edition, self-publication.
2. Gunasekaran. P (2007). Laboratory manual in Microbiology. New age international publisher.
3. Elsa Cooper (2018). Microbial Physiology: A Practical Approach. Callisto Reference publisher.
References
1.James G Cappucino and N. Sherman MB (2013). A lab manual. Pearson Benjamin Cummins
Publisher.
2.Michelle McNeil.M.A, Elizabeth Sharpe-Aparicio, M.S. Victoria Sharpe (2013). Microbiology
Laboratory Manual, Second Edition. Publisher: BLUEDOOR 9781599845302
3.Aneja. K. R, (2018). Laboratory Manual of Microbiology and Biotechnology 2nd Edition, Medtech
Publishers.
4.Abdul Jaffar Ali (2017). Microbiology Laboratory Manual, ISBN 9789394681712
5.Cappucino and Welsh (2018). Microbiology-A laboratory manual, 12th edition, Pearson publisher.
Suggested Reading
1. Aneja.K.R. (2005). Experiments in Microbiology, plant pathology and biotechnology, Fourth
edition.
New age international publishers.
2. Dubey.R.C., Maheswari.D.K (2002). Practical Microbiology. S.Chand publishing
Web Resources
1. https://archive.nptel.ac.in/courses/102/103/102103015/
2. https://microbenotes.com/carbohydrate-fermentation-test/
3. https://step1.medbullets.com/microbiology/104012/pigment-producing-bacteria
 Course Articulation Matrix

Programme Outcomes
Programme Specific Outcomes
Cognitive Level
Course
Outcomes
PO1 PO2 PO3 PO4 PO5 PO6 PO7 PO8 PSO1 PSO2 PSO3 PSO4 PSO5
K1
CO 1 2 3 3 1 3 2 2 - 3 3 3 - -

K2
CO 2 2 3 3 2 3 2 2 2 3 3 3 - -

K3
CO 3 2 3 3 2 3 3 3 - 3 3 3 - -

K4
CO 4 2 3 3 2 3 2 2 - 3 3 3 - -

K5
CO 5 2 3 3 2 3 3 1 - 3 3 2 - -

Wt. Avg. 2 3 3 1.8 3 2.4 2 2 3 3 2.8 - -

Wt. Avg. 2.7 2.9

Overall Mapping of the Course With PO 2.7

With PSO 2.9
 Semester II - Allied Theory
Biochemistry –II

Course Code
Credits 4
Hours / Cycle 4
Category Part Allied Theory
Semester II
Year of From the academic year 2023-2024
Implementation
1.To understand enzyme kinetics and the mechanisms of enzyme
action
2.To know the metabolic pathways of carbohydrates and lipids
Course Objectives 3.To differentiate oxidative and substrate level phosphorylation
4.To understand the biosynthesis of amino acids
5.To study about the biosynthesis and degradation of nucleic acids

Bloom’s
Taxonomy
PSO
CO # Course Outcome(s) Levels
Addressed
(K1 to K5)

On completing the course successfully, the student will be able to

CO 1 Relate various metabolic pathways PSO1 K1

with energetics and recall about PSO3 PSO5
enzymes and their functions
CO 2 Summarize the various metabolic PSO1 K2
pathways and mechanisms of enzyme PSO3 PSO4
action
CO 3 Apply the concepts of enzyme PSO1 K3
kinetics in metabolism PSO3 PSO4
CO 4 Discover the significance of PSO1 K4
metabolic pathways for normal body PSO3 PSO4
functions, and enzyme action
CO 5 Evaluate the biosynthesis and PSO1 K5
degradation of biomolecules with PSO3
enzyme kinetics.

SYLLABUS
UNIT CONTENT HOURS COs BLOOM’S
60 TAXONOMY
LEVEL
 I Enzymes – Classification and 13 1,2,3,4, K1,2,3,4,5
nomenclature - Mechanism of 5
enzyme action. Michaelis-Menten
Equation, Lineweaver-Burk plot,
factors influencing enzyme activity.
Enzyme inhibition – Competitive,
non-competitive and uncompetitive.

II Basic concepts of metabolism. 13 1,2,3,4, K1,2,3,4,5

Carbohydrates: Glycolysis, TCA 5
cycle, gluconeogenesis and glycogen
metabolism. Electron transport chain,
oxidative phosphorylation and
substrate-level phosphorylation.

III Lipid metabolism – Biosynthesis of 11 1,2,3,4, K1,2,3,4,5

fatty acids, cholesterol biosynthesis, 5
degradation of fatty acids -β oxidation
and omega oxidation

IV Protein metabolism - 11 1,2,3,4, K1,2,3,4,5

Transamination, oxidative and non - 5
oxidative deamination,
decarboxylation and urea cycle.
Biosynthesis of nonessential amino
acids - Asparagine, Glutamine,
Serine
V Nucleotide metabolism – 12 1,2,3,4, K1,2,3,4,5
Biosynthesis of purine and pyrimidine 5
bases – De novo and salvage pathway.
Degradation of purine and pyrimidine
bases.
Prescribed Books/Textbooks
1.Jain, J.L., Jain, S. and Jain, N. (2016). Fundamentals of Biochemistry, S. Chand and
Company Ltd.
2.Victor W. Rodwell. (2018). “Harper’s Illustrated Biochemistry”. 31st Edition. McGraw-
Hill
References
1.David E Metzler: Biochemistry. (2003). The Chemical reactions of Living Cells Vol.1,
2nd Edition,
Elsevier Academic Press.
2.Berg JM, Tymoczko JL and Stryer L. (2019). Biochemistry, 9th Edition, WH Freeman
and Company,
3.Voet, D.J and J.G. Voet and C.W. Pratt. (2010). Principles of Biochemistry. 3rd Edition,
John Wiley & Sons Inc.
4.Plummer DT. (2017). An Introduction to Practical Biochemistry. Tata McGraw-Hill
Publishing Company Limited
 Suggested Reading
1. Trevor Palmer. Philip Bonner. (2008). Enzymes, WordPress.
2. Mahalakshmi. V (2009). Enzyme Technology. Shantha Publications.
3. Satyanarayan, U. Chakrapani (2021). Biochemistry, 6th Ed. Elsevier
Web Resources
1.https://www.biologydiscussion.com/metabolism/metabolism-of-lipids-5-cycles-
withdiagram/11219
2. https://microbiologynotes.org/enzymes-introduction-enzyme-activity-and-
workmechanism/
3. https://archive.nptel.ac.in/courses/104/105/104105076/

Course Articulation Matrix

Programme Outcomes
Programme Specific Outcomes
Cognitive Level
Course
Outcomes
PO1 PO2 PO3 PO4 PO5 PO6 PO7 PO8 PSO1 PSO2 PSO3 PSO4 PSO5
K1
CO 1 3 3 3 - - - 2 2 3 - 3 - 3

K2
CO 2 3 3 3 - - - 2 2 3 - 3 2 -

K3
CO 3 3 3 3 - - - 2 2 3 - 3 2 -

K4
CO 4 3 3 3 - - - 2 2 3 - 3 2 -

K5
CO 5 3 3 3 - - - - - 3 - 3 - -

Wt. Avg. 3 3 3 - - - 2 2 3 - 3 2 3

Wt. Avg. 2.6 2..8

Overall Mapping of the Course With PO 2.6

With PSO 2.8
 Semester II - Allied Practical
Biochemistry –II
Course
Code
Credits 1
Hours / 2
Cycle
Category Part Allied Practical

Semester II
Year of From the academic year 2023-2024
Implement
ation
1.To provide hands-on training in titrations
2.To acquire the knowledge on colorimetric estimations
Course 3.To confirm the results of volumetric and colorimetric analysis
Objectives 4.To estimate the amount of biological sample
5.To enable students to take up jobs in diagnostic and research laboratories

Bloom’s
PSO Taxonomy
CO # Course Outcome(s)
Addressed Levels
(K1 to K5)

On completing the course successfully, the student will be able to

CO 1 Recall and list the procedures for quantitative PSO1 K1

estimation of biological substances PSO3

CO 2 Explain the materials and requirements for PSO1 K2

volumetric and colorimetric analyses PSO3 PSO5
CO 3 Experiment with volumetric and colorimetric PSO1 K3
methods to know the concentration of PSO5
biological samples
CO 4 Analyse the experimental data obtained PSO1 K4
through quantitative analyses PSO3PSO4
PSO5
CO 5 Discuss the results with reference to clinical PSO1 K5
significance PSO3PSO5

SYLLABUS
 UNIT CONTENT HOURS COs BLOOM’S
30 TAXONOMY
LEVEL
I Volumetric analysis 6 1,2,3,4,5 K1,2,3,4,5
1.Estimation of glucose by Benedict’s
method
2. Estimation of glycine by Sorenson’s
formal titration method
II 6 1,2,3,4,5 K1,2,3,4,5
3. Estimation of Iron using KMnO4 as link
solution and ferrous ammonium sulphate
as standard.

4. Estimation of Ascorbic acid by 2, 6

dichlorophenol – indophenols dye method
III Colorimetric estimation 6 1,2,3,4,5 K1,2,3,4,5
Estimation of glucose by Enzymatic
method
Estimation of protein by Biuret method
IV Estimation of cholesterol by Zak’s method 6 1,2,3,4,5 K1,2,3,4,5
Estimation of creatinine by Jaffe’s method

V Estimation of urea by Dam method 6 1,2,3,4,5 K1,2,3,4,5

Validate the results of volumetric and
colorimetric analysis with clinical
significance.
Prescribed Books/Textbooks
1. Plummer DT An Introduction to Practical Biochemistry. (2017). Tata McGraw-Hill
Publishing Company Limited
2. Randhir Singh and S. K. Sawhney (2014). Introductory Practical Biochemistry,10th
reprint. Narosa Publishers
References
1. Kaushik.GG, Neha Sharma, Sabira Dabeer, Ruchi Jindal (2020). Practical Manual of
Biochemistry, CBS Publishers.
2. Geetha Damodaran Practical Biochemistry (2016) Jaypee Brothers Medical
Publishers (P) Ltd
3. Jayaraman J (2011). Laboratory Manual in Biochemistry, 2nd Edition. Wiley Eastrn
Ltd., New Delhi
4. Berg JM, Tymoczko JL and Stryer L. (2019). Biochemistry, 9th Edition, WH
Freeman and Company
Suggested Reading
1. DM Vasudevan Sudhir Kumar Das (2020) Practical Textbook of Biochemistry for
Medical Students Jaypee Brothers Medical Publishers (P) Ltd
2. Punit Puri (2021). Techniques in biochemistry. Walnut Publication
 Web Resources
1.https://www.academia.edu/4836805/Volumetric_Analysis_Glycine_Acid_NO_Iodine_N
o_etc
2.www.cdc.gov/nchs/data/nhanes/nhanes_03_04/l13_c_met_lipids.pdf
3.https://allmedtests.com/determination-blood-urea-dam-method/

Course Articulation Matrix

Programme Outcomes
Programme Specific Outcomes
Cognitive Level
Course
Outcomes
PO1 PO2 PO3 PO4 PO5 PO6 PO7 PO8 PSO1 PSO2 PSO3 PSO4 PSO5
K1
CO 1 3 3 3 - - - - - 3 - 2 - -

K2
CO 2 3 3 3 - - - 2 3 3 - 3 - 2

K3
CO 3 3 3 - - - - 2 3 3 - - - 2

K4
CO 4 3 3 3 - - - 2 3 3 - 2 3 2

K5
CO 5 3 3 3 - - - 2 3 3 - 3 2

Wt. Avg. 3 3 3 - - - 2 3 3 - 2.5 3 2

Wt. Avg. 2.8 2.6

Overall Mapping of the Course With PO 2.8

With PSO 2.6
 Semester III - Core Theory
Immunology
Course Code

Credits 5
Hours / Cycle 6
Category Part Core Theory
Semester III
Year of From the academic year 2023-2024
Implementation
1.To know about the organs and cells of immune system.
2.To learn about types of antigen and antibodies
Course Objectives 3.To acquire knowledge on concepts of immune mechanisms.
4.To perform various methods of Immunodiagnosis.
5.To gain knowledge on Immunoprophylaxis.

Bloom’s
PSO Taxonomy Levels
CO # Course Outcome(s)
Addressed (K1 to K5)

On completing the course successfully, the student will be able to

CO 1 Define concepts of immunity and immunodiagnosis. PSO1, PSO2, K1

PSO4
CO 2 Demonstrate the mechanisms involved in immunity PSO1, PSO2, K2
and immunodiagnosis PSO3
CO 3 Apply practical skills to perform basic PSO1, PSO2, K3
immunological methods of diagnosis PSO3, PSO4
CO 4 Discover the effects of the immune system and to PSO1, PSO2, K4
study the importance of vaccines PSO3, PSO4,
PSO5
CO 5 Explain the various aspects of the immune system in PSO1, PSO2, K5
promoting human health. PSO3, PSO4,
PSO5

SYLLABUS
UNIT CONTENT HOURS COs BLOOM’S
90 TAXONOMY
LEVEL
 I Introduction to Immunology. Immunity and its 20 1,2,3,4 K1,2,3,4,5
types-innate and adaptive. Structure and functions ,5
of the organs and cells of the immune system.

II Antigen – properties and types of antigens. 20 1,2,3,4, K1,2,3,4,5

Immunogen, Adjuvant, and Hapten. 5
Immunoglobulins – structure, types, properties and
its functions. Monoclonal and polyclonal antibodies
production and its applications.
III Introduction to Major histocompatibility complex 20 1,2,3,4, K1,2,3,4,5
(MHC). Humoral and Cell mediated immune 5
response. Complement – the classical and alternate
pathways. Role of cytokines.

IV Antigen – Antibody reactions: principles and 13 1,2,3,4, K1,2,3,4,5

applications – Agglutination, and Precipitation. 5
Complement fixation, Immunofluorescence – direct
and indirect Immunoelectrophoresis, ELISA, RIA,
and Western blotting. Skin test (Mantoux test).

V Hypersensitivity – definition, types and 17 1,2,3,4, K1,2,3,4,5

characteristics. Autoimmunity – mechanism and 5
the related diseases. Immunoprophylaxis –
Vaccines and its types. Immunization schedule and
its importance in public health.

Prescribed Books/Textbooks:
1.Martin Flajnik, Nevil J.Singh, Steve M.Holland (2020). Pauls Fundamental Immunology, 8th Edition,
LWW publishing.
2.Peter Parham (2014), The immune system, 4th edition, Garland Science Publisher.
3.LawrenM.Sompayrac (2019). How the Immune system works. Wiley Blackwell Publisher.
4.Mark Peakman,Diego Vergani(2009). 2nd Edition, Basic &Clinical Immunology, Churchill
Livingstone Publisher.
5.Maurice R.G. O'Gorman, Albert D. Donnenberg, (2008), 2nd edition Hand book of Human
Immunology, C.R.C Press.
References:
1.Roitt’s (2017). Essential Immunology, 13th edition. Blackwell Scientific Publishers.
2.Abul Abbas Andrew, H. Lichtman and Shiv Pillai. (2021). Cellular and Molecular Immunology, 10 th
edition, Elsevier.
3.Noel R. Rose, Editors: Robert G. Hamilton and Barbara Detric K. (2002). Manual of Clinica
Laboratory and Immunology, 6th Edition.ASM Publications Company.
 Suggested Reading:
1.Ananthanarayanan. R and Jayaram Panikaer, C.K. (2020). Textbook of Microbiology, 11th edition.
Orient Longman.
2.R.A. Goldsby, T.J. Kindt and B.A. Osborne, Kuby Immunology (2020). W.H. Freeman and
Company.
Web Resources:
1.https://www.immunology.org/public-information/bitesized-immunology
2.https://www.aai.org/Education/Teaching-Resources
3.http://www.helmberg.at/

Course Articulation Matrix

Programme Outcomes
Programme Specific Outcomes
Cognitive Level
Course
Outcomes
PO1 PO2 PO3 PO4 PO5 PO6 PO7 PO8 PSO1 PSO2 PSO3 PSO4 PSO5
K1
CO 1 3 3 - 3 3 3 3 3 3 3 - 3 -
K2
CO 2 3 3 3 2 3 3 3 - 3 3 3 -
K3
CO 3 3 2 3 3 3 3 3 3 2 3 2 3 -
K4
CO 4 3 3 3 2 3 3 3 2 3 3 2 3 3
K5
CO 5 3 3 2 3 2 2 2 2 3 3 3 3 2

Wt. Avg. 3 2.8 2.7 2.6 2.8 2.8 2.8 2.5 2.8 3 2.5 3 2.5
2.8
Wt. Avg. 2.8

Overall Mapping of the Course With PO 2.8

With PSO 2.8
 Semester III – Core Practical
Immunology
Course Code

Credits 3
Hours / Cycle 2
Category Part Core Practical
Semester III
Year of From the academic year 2023-2024
Implementation
1.To enable the students to learn about the different types of blood cells.
2.To perform agglutination reactions.
3.To learn the concepts of precipitation reactions.
Course Objectives
4.To understand the principle of immunoelectrophoresis.
5.To learn about the detection of antigen and antibody through ELISA
technique.

Course Outcome BLOOM’S

(Upon completion of this course, students PSOs TAXONOMY LEVEL
CO No.
will be able to) Addressed

List and recall the procedures for PSO1, PSO2,

CO1 K1
immunology practical. PSO 3
Explain about the materials, equipment
PSO1, PSO2,
CO2 required for immunodiagnosis. K2
PSO3, PSO4

Apply the acquired skills in PSO1, PSO 2,

CO3 K3
immunodiagnosis. PSO 3
Analyse the various methods used in PSO1, PSO2,
CO4 immunodiagnosis and the experimental PSO3, PSO 4 K4
results.
Recommend a suitable technique based on PSO1, PSO2,
CO5 K5
the requirement. PSO3, PSO4

SYLLABUS
UNIT CONTENT HOURS COS BLOOM’S
30 TAXONOMY
LEVEL

1 Preparation of plasma and serum. 3 1,2,3,4,5 K1,2,3,4,5

Peripheral blood smear and differential
staining.
2 Agglutination reactions 10 1,2,3,4,5 K1,2,3,4,5
a. Febrile - Widal test
b. Latex - ASO, RA and CRP test.
 Haemagglutination - Blood grouping & Rh
typing.
3 Flocculation - RPR test 10 1,2,3,4,5 K1,2,3,4,5
Precipitation reactions in gel
a. Ouchterlony double immunodiffusion,
b. Single radial immunodiffusion.
4 Precipitation in agar with an electric field 2 1,2,3,4,5 K1,2,3,4,5
a. Immunoelectrophoresis
b. Counter immunoelectrophoresis.
5 ELISA (Demonstration only) 5 1,2,3,4,5 K1,2,3,4,5
Prescribed Books/Textbooks:
1.Idris Adewale Ahm (2018). Laboratory Manual - Basic Immunology. Ed. Lincoln University
Malaysia publishers.
2.Suhad Hadi Mohammed, Mohanad Mohsin Ahmed (2019). Practical Immunology. Lambert
Academic publishers.
References:
1.Frank C. Hay, Olwyn M.R. Westwood (2002). Practical Immunology, 4th edition, John Wiley
& Sons, Inc.
2.G.P. Talwar & S.K. Gupta (2017). A Handbook of Practical and clinical Immunology, volume
2-CBS Publishers.
Suggested Reading:
1.Tobili Y. Sam-Yellowe (2021). Immunology: Overview and Laboratory Manual, Springer
Publishers.
2.Karthik Kaliaperumal, Senbagam Duraisamy, Senthilkumar Balakrishnan (2017). Practical
Immunology A Laboratory Manual, LAP LAMBERT Academic Publishing.
Web Resources:
1.https://www.inside-immunity.org/en.php
2.https://www.niaid.nih.gov/research/immune-system-disorders
3.https://webstor.srmist.edu.in/web_assets/downloads/2021/18BTC106J-lab-manual.pdf
 Course Articulation Matrix

Programme Outcomes
Programme Specific Outcomes
Cognitive Level
Course
Outcomes
PO1 PO2 PO3 PO4 PO5 PO6 PO7 PO8 PSO1 PSO2 PSO3 PSO4 PSO5

CO 1 2 3 3 3 3 3 3 - 3 - 3 - - K1

CO 2 2 2 3 3 3 3 3 2 3 2 - 3 - K2

CO 3 3 2 2 3 3 3 3 - - 2 2 - - K3

CO 4 2 3 3 3 3 3 3 2 3 3 - 3 2 K4

CO 5 3 2 3 3 3 2 2 2 - 3 2 3 - K5

Wt. Avg. 2.4 2.4 2.8 3 3 2.8 2.8 2. 3 2.5 2.3 3 2

2.6
. 2.7

Overall Mapping of the Course With PO 2.7

With PSO 2.6
 Semester III – Allied Theory
Bioinstrumentation
Course Code

Credits 4
Hours / Cycle 4
Category Part Allied Theory
Semester III
Year of From the academic year 2023 -2024
Implementation
1.To understand the basic principles of analytical instruments used in the
field of biological sciences.
2.To know about the types of centrifuges.
Course Objectives 3.To gain knowledge about principles of spectroscopy.
4.To get insight on Chromatography and electrophoretic techniques.
5.To understand about radio isotopic techniques and their applications.

Bloom’s
PSO Taxonomy Levels
CO # Course Outcome(s)
Addressed (K1 to K5)

On completing the course successfully, the student will be able to

CO 1 List the use of basic instruments in the microbiology PSO1, PSO2, K1

laboratory PSO3& PSO4
CO 2 Demonstrate the acquired skills in operating PSO1, PSO2 K2
instruments PSO3&PSO5
CO 3 Select the appropriate technique for an experiment PSO1, PSO2, K3
PSO3, PSO4
CO 4 Compare between the different methods of PSO1, PSO2, K4
instrumentation PSO3, PSO5
CO 5 Decide and formulate standard operating PSO1, PSO2, K5, K6
procedures. PSO3&PSO5
 SYLLABUS
UNIT CONTENT HOURS COs BLOOM’S
60 TAXONOM
Y LEVEL
I Basic instruments in microbiology - pH meter, 12 1,2,3,4,5 K1,2,3,4,5
Principle and applications of autoclave, hot air oven,
Laminar air flow and Incubators. Buffers of
biological importance- Phosphate, Acetate, TE,
TAE- calculation of Normality, molality and
molarity.
II Centrifugation Technique – Principle (concept of g 12 1,2,3,4,5 K1,2,3,4,5
and rpm) and types of centrifugations - Preparative
and analytical, Svedberg unit. Types of rotors and
Centrifuges, Cooling centrifuges.

III Electrophoresis Techniques: Starch Gel, AGE, 12 1,2,3,4,5 K1,2,3,4,5

SDSPAGE. Radio isotopes - Geiger Muller counter,
Scintillation counter and Autoradiography.
.
IV Chromatography Techniques: Principle, 12 1,2,3,4,5 K1,2,3,4,5
instrumentation and applications of Chromatography
– Column, Paper, Thin layer, Gas
liquid chromatography, HPLC.
V Colorimetry - Principle, instrumentation and 12 1,2,3,4,5 K1,2,3,4,5
applications. Spectroscopy - visible and UV
spectrophotometry, Mass spectroscopy.
Spectrofluorimetry, Flame photometry
Prescribed Books/Textbooks
1.Ponmurugan. P and Gangathara PB (2012). Biotechniques.1st edn. MJP publishers.
2.Veerakumari, L (2009). Bioinstrumentation- 5th Edition -.MJP publishers.
3.Upadhyay, Upadhyay and Nath (2002). Biophysical chemistry – principles and techniques 3rd
Edition. Himalaya publishing home.
References
1. Rodney.F.Boyer (2000). Modern Experimental Biochemistry, 3rd Edition. Pearson Publication.
2.Skoog.A., West. M (2014). Principles of Instrumental Analysis – 14th Edition W.B.Saunders Co.,
Philadelphia.
3.N. Gurumani. (2006). Research Methodology for biological sciences- 1st Edition – MJP Publishers.
4.Wilson K, and Walker J (2018). Principles and Techniques of Biochemistry and Molecular Biology.
8th edition. Cambridge University Press.
5. McHale J.L. (2008). Molecular Spectroscopy. 1st edition. Pearson
Suggested Reading
1.Pingoud A., Urbanke C., et al. (2002). Biochemical Methods – A concise guide for Students and
researchers. Wiley publication.
2.Webster, J.G. (2004). Bioinstrumentation- 4th Edition - John Wiley & Sons (Asia) Pvt. Ltd,
Singapore.
Web Resources:
1. https://archive.nptel.ac.in/courses/104/105/104105102/
2. https://archive.nptel.ac.in/courses/102/101/102101050
3. https://www.classcentral.com/course/swayam-analytical-techniques-13896
4. https://archive.nptel.ac.in/courses/102/103/102103017/

Course Articulation Matrix

Programme Outcomes
Programme Specific Outcomes
Cognitive Level
Course
Outcomes
PO1 PO2 PO3 PO4 PO5 PO6 PO7 PO8 PSO1 PSO2 PSO3 PSO4 PSO5
K1
CO 1 2 3 3 2 3 2 3 - 3 3 3 3 -

K2
CO 2 2 3 3 2 3 2 3 2 3 3 3 - 3

K3
CO 3 2 3 3 2 3 2 3 2 3 3 3 - 3

K4
CO 4 2 3 3 2 3 3 3 2 3 3 3 - 3

K5, K6
CO 5 3 3 3 - - - - - 3 3 3 - -

Wt. Avg. 2.2 3 3 2 3 2.3 3 2 3 3 3 3 3

Wt. Avg. 2.6 3

Overall Mapping of the Course With PO 2.6

With PSO 3
 Semester III – Allied Practical
Bioinstrumentation
Course Code
Credits 1
Hours / Cycle 2
Category Part Allied Practical
Semester III
Year of From the academic year 2023-2024
Implementation
1. To impart hands-on training in handling basic instruments.
2. Prepare various buffers for the practical.
Course Objectives 3. Make use of Colorimeter and spectrometer for estimation purposes.
4. To separate biomolecules by chromatography and electrophoresis.
5. To separate cellular components by centrifugation.

Bloom’s
PSO Taxonomy Levels
CO # Course Outcome(s)
Addressed (K1 to K5)

On completing the course successfully, the student will be able to

CO 1 Recall and write the principles and procedures PSO1, PSO2 K1

pertaining to bioinstrumentation & PSO3
CO 2 Relate the various apparatus, equipment and PSO1, PSO2, K2
materials of use in biological sciences &PSO3

CO 3 Experiment with various bioinstrumentation PSO1, PSO2, K3

techniques PSO3 &
PSO5
CO 4 Test for biological samples using various PSO1, PSO2 K4
instruments. & PSO3
CO 5 Evaluate various bioinstrumentation techniques and PSO1, PSO2, K5, K6
their outputs PSO3 &
PSO5
 SYLLABUS
UNIT CONTENT HOURS COs BLOOM’S
30 TAXONOMY
LEVEL
I Usage and calibration of Micropipettes. 7 1,2,3,4,5 K1,2,3,4,5
pH metry: Preparation of buffers- Acidic and
alkaline range, Calibration of pH meter.
Preparation of molar, molal and Normal solutions

II Colorimetry / Spectrophotometry - Protein and 8 1,2,3,4,5 K1,2,3,4,5

Carbohydrate estimation

III Paper chromatography, Thin layer chromatography, 9 1,2,3,4,5 K1,2,3,4,5

Electrophoresis of Proteins – Serum electrophoresis
IV Separation and determination of dry weight of 6 1,2,3,4,5 K1,2,3,4,5
bacterial cells by differential
centrifugation – conversion of rpm to g
Counter current separation using immiscible
solvents
V Drafting a protocol for an experiment - 1,2,3,4,5 K1,2,3,4,5

Prescribed books/Text books

1.Jayaraman J (2011). Laboratory Manual in Biochemistry, 2nd Edition. Wiley Eastrn Ltd., New Delhi.
2. Douglas A. Skoog, F. James Holler, Stanley R. Crouch (2016). Principles of Instrumental Analysis.
Cengage Learning, 6th Ed. ISBN: 978-0495012016.

References:
1.Keith Wilson, John Walker (2010). Principles and techniques of Biochemistry and
Molecular Biology,7th ed, Cambridge University Press India Pvt.Ltd. ISBN 1-4051-3544-1.
2.Sharma.B., Kokyo and Keiichiro Fuwa (2016). Instrumental Methods of Chemical Analysis- Recent
Advances in Analytical Spectroscopy. Pergamon; 1st ed. ISBN: 978-0080262215.
3.Chung Chow Chan, Herman Lam, Xue-Ming Zhang. (2010) Practical Approaches to Method
Validation and Essential Instrument Qualification,1st ed. Wiley; ISBN: 978- 0470121948.
4.Himanshu Sharma, Pramod K. Singh (2018). Laboratory Manual for Bioinstrumentation,
Biochemistry, Microbiology, Cell Biology and Enzyme Technology for PG and UG students. Excellent
Publishing House ISBN: 978-93-86238-53-5
Suggested Reading
1. Punit Puri (2021). Techniques in biochemistry. Walnut Publication.
2.James M.Miller (2009). Chromatography: Concepts and Contrasts, 1 st edition, ISBN:9780471472070
Web Resources
1. https://onlinecourses.nptel.ac.in/noc23_cy09/preview
2. https://nptel.ac.in/courses/103105060
3. https://nptel.ac.in/courses/103108100
4.https://www.coursera.org/lecture/forensic-science/week-2b-1-introduction-tochromatography-MSip0
 Course Articulation Matrix

Programme Outcomes
Programme Specific Outcomes
Cognitive Level
Course
Outcomes
PO1 PO2 PO3 PO4 PO5 PO6 PO7 PO8 PSO1 PSO2 PSO3 PSO4 PSO5
K1
CO 1 3 3 3 3 3 3 3 - 3 3 3 - -

K2
CO 2 3 3 3 3 3 3 3 - 3 3 3 - 3

K3
CO 3 2 3 3 3 3 3 3 3 3 3 3 - 3

K4
CO 4 2 3 3 3 3 3 3 - 3 3 3 - -

K5, K6
CO 5 3 3 3 1 3 3 1 2 3 3 3 - 3

Wt. Avg. 2.6 3 3 2.6 3 3 2.6 2.5 3 3 3 - 3

Wt. Avg. 2.8 3

Overall Mapping of the Course With PSO 3

With PO 2.8
 Semester III & IV – Interdisciplinary Theory
Health and Nutrition
Course Code
Credits 3
Hours / Cycle 4
Category Part Interdisciplinary Theory
Semester III
Year of From the academic year 2023 -2024
Implementation
1.To gain knowledge on the holistic concept and different dimensions of
health
Course 2. To understand the objectives of public health
Objectives 3. To learn about nutrition its importance in human health.
4.To be familiar with the nutritional guidelines for staying fit
5. To get insights on community health care

Bloom’s Taxonomy
PSO Levels
CO # Course Outcome(s)
Addressed (K1 to K5)

On completing the course successfully, the student will be able to

CO 1 Relate the determinants and indicators of PSO1, K1

health, nutrition and fitness. PSO3,
PSO4
CO 2 Summarize the significance of health and PSO1, K2
nutrition in prevention of diseases. PSO3,
PSO4
CO 3 Identify the importance of health and nutrition PSO 1, K3
for staying fit. PSO 3
CO 4 Discover the role of physical activity and PSO1, K4
nutrient supplements for determining overall PSO 3,
health PSO 4
CO 5 Recommend practices to lead a healthy life. PSO 1, K5
PSO 3,
PSO 4,

SYLLABUS
UNIT CONTENT HOURS COs BLOOM’S
60 TAXONOMY
LEVEL
 I Holistic concept of health: Introduction, 10 1,2,3,4 K1,2,3,4,5
determinants of health and its indicators. ,5
Social determinants of health. Dimensions
of health - physical, mental, emotional,
social, moral and spiritual. Health care
services and policies.

II Public health- objectives, approach and 10 1,2,3,4, K1,2,3,4,5

components of public health. Health 5
education, disease diagnosis and
rehabilitation. Holistic Medicines for disease
prevention. Functions of government and
Public Health Organisation.
III Nutrition and its importance: Nutrients- 15 1,2,3,4, K1,2,3,4,5
carbohydrates, lipids, proteins, vitamins, 5
minerals, water, dietary fibre. Balanced diet.
Diseases related to excessive and inadequate
intake of nutrients. Calorie intake, methods
of cooking and preventing nutrient loss.
Nutrients for special conditions- sports,
malnourished and undernourished children,
pregnant and lactating women.
IV Fitness: Definition of fitness, approaches for 10 1,2,3,4, K1,2,3,4,5
keeping fit, nutritional guideline and 5
supplements. Physical activity- frequency,
intensity, time type, importance. Physical
activity pyramid. Health complication of
overweight and obesity, BMI, planning
weight reducing diets.
V Community Health care -Education and 15 1,2,3,4, K1,2,3,4,5
training in maternal and child health- School 5
health services. Geriatric care and welfare of
aged- mental health- health services through
general practitioners. Environmental
sanitation and sanitation in public places
Prescribed Books/Textbooks
1. Bappco. (2021). Swaminathan Hand book of Food and Nutrition (2021) Standard Edition
2. Kaveri Chakrabarty, A. S. Chakrabarty (2020). Textbook of Nutrition in Health and Disease Springer
Singapore.
References
1. Bamji, M.S., K. Krishnaswamy; G.N.V. Brahmam (2009). Textbook of Human
Nutrition (3rd edition) Oxford and IBH Publishing Co. Pvt. Ltd.
2. Vijaya Khader (2002). Food, nutrition and health, Kalyan Publishers.
3. Srilakshmi, B. (2010) Food Science, (5th Edition). New Age International Ltd.
 Suggested Reading
1. Douglas C. Heimburger and Jamy D Ard (2006). Handbook of Clinical Nutrition. 4 th Edition
Elsevier
2. Diana Guzys, Rhonda Brown. (2020). An Introduction to Community and Primary Health Care 3rd
edition. Cambridge University Press
Web Resources
1.https://nhm.gov.in/index1.php?lang=1&level=1&sublinkid=149&lid=2
25
2. https://www.who.int/hia/about/faq/en/
3. https://www.nhp.gov.in/healthylivingViewall

Course Articulation Matrix

Programme Outcomes
Programme Specific Outcomes
Cognitive Level
Course
Outcomes
PO1 PO2 PO3 PO4 PO5 PO6 PO7 PO8 PSO1 PSO2 PSO3 PSO4 PSO5
K1
CO 1 2 3 3 - - - 3 3 2 - 3 3 -

K2
CO 2 2 3 3 - - - 3 3 3 - 3 3 -

K3
CO 3 2 3 3 - - - - - 3 - 3 - -

K4
CO 4 2 3 3 - - - 3 3 3 - 3 3 -

K5
CO 5 2 3 3 - - - 2 3 3 - 3 3 -

Wt.Avg 2 3 3 - - - 2.5 3 3 - 3 3 -

Wt. Avg. 2.7 3

Overall Mapping of the Course With PO 2.7

With PSO 3
 Semester IV-Core Theory
Microbial Genetics and Molecular biology

Course Code
Credits 5
Hours / Cycle 6
Category Part Core Theory
Semester IV
Year of From the academic year 2023-2024
Implementation
1.To understand the basic concepts of Microbial genetics and molecular biology.
2.To learn gene transfer mechanisms in bacteria.
3.To study the concept of central dogma of molecular biology and genetic code.
4.To provide in depth knowledge in gene expression and regulation in
Course
prokaryotes.
Objectives
5.To learn the importance of Mutations and carcinogenicity testing of mutagens
by Ames test.

Bloom’s Taxonomy
PSO Levels
CO # Course Outcome(s)
Addressed (K1 to K5)

On completing the course successfully, the student will be able to

CO 1 Recall basic knowledge in microbial genetics, PSO1, PSO2 K1

molecular biology and rDNA technology. PSO3 PSO4,
PSO5
CO 2 Explain the basic concepts in the field of PSO1, PSO2, K2
microbial genetics, molecular biology and PSO3, PSO4,
rDNA technology. PSO5

CO 3 Apply the knowledge to study the structure, PSO1, PSO2 K3

functions and transfer of genes in microbes. PSO3, PSO4,
PSO5
CO 4 Examine and analyse the mechanisms of gene PSO1, K4
expression, regulation and mutation in in vivo PSO2 PSO3,
and in vitro conditions PSO4
CO 5 Assess the prospects and significance of PSO1, PSO2, K5
molecular genetics, molecular biology and PSO3, PSO4,
rDNA technology in microbiology. PSO5
 SYLLABUS

UNIT CONTENT HOURS COs BLOOM’S

90 TAXONOMY
LEVEL
I Historical perspectives, Discovery of DNA and RNA 17 1,2,3, K1,2,3,4,5
as the genetic material, Primary, secondary and 4,5
tertiary structure of DNA, Denaturation and
renaturation of DNA, Types of RNA, secondary and
tertiary structure of RNA.

II Organization of genome in prokaryotes and 17 1,2,3,4 K1,2,3,4,5

eukaryotes, Extrachromosomal genetic material – ,5
Plasmids –types and structure, Prokaryotic DNA
replication – models-rolling circle, looped rolling
circle, sigma and linear DNA replication,
semiconservative mode of replication, Theta
replication in E. coli - Enzymology of replication,
initiation, elongation and termination, fidelity of
replication and inhibitors of replication.

III Gene transfer mechanism in bacteria- transformation, 18 1,2,3,4 K1,2,3,4,5

conjugation and transduction. Mutations- ,5
spontaneous and induced, Mutagens – types and their
mode of action, Carcinogenicity testing of mutagens
by Ames test.

IV Gene Expression-Central dogma of molecular 19 1,2,3, K1,2,3,4,5

biology, transcription in prokaryotes-RNA 4,5
polymerases, promoter, initiation, elongation and
termination of transcription, post transcriptional
modifications in prokaryotes (tRNA and rRNA).
Genetic code Translation in prokaryotes. Regulation
of genes in prokaryotes-The lactose and
Tryptophan operon

V Introduction to rDNA technology, Tools of genetic 19 1,2,3,4 K1,2,3,4,5

engineering – Restriction enzymes, linkers and ,5
adaptors. Vectors - plasmid, phage cosmid, phagemid
and shuttle vectors, Basic steps in Gene cloning.

Prescribed Books/Textbooks
1.David Freifelder. (1995). Molecular Biology. Narosa Publ. House.
2.David Freifelder, John Cronan and Stanly R. Maloy.(2009). Microbial Genetics. Narosa publishing
house.
3.Sivarama Sastry, Padmanaban. G and Subarmanyam. C. (1994). Text Book of Molecular Biology.
Macmillan India Ltd.
4.T. A Brown. (2006). Gene Cloning and DNA Analysis – An Introduction, 5th edition.
 Blackwell Publication.
5.S.B. Primrose and R.M. Twyman. (2008). Principles of Gene Manipulation and Genomics. 7 th edition.
Blackwell publication.

References
1. Lodish. (2003). Molecular cell biology. Scientific American books.
2.James. D. Watson. (2013). Molecular biology of the Gene. Pearson Education.
3. Hofmann. A. (2018). Wilson and Walkers Principles and techniques of Biochemistry and Molecular
biology. Cambridge University Press.
Suggested Reading
1. William S. Klug & Michael R. (2007). Concepts of Genetics. Cummings.Darling Kindergluy.
2. Gardner, Simmons and Snustad. (2006). Principles of Genetics. Wiley Student Edition.
3. R.M. Twyman. (1998). Advanced Molecular Biology: A Concise Reference. Viva Books Pvt ltd.
Web Resources
1. https://www.scienceprofonline.com/vmc/microbial-genetics-main.html
2. https://study.com/academy/topic/microbial-genetics.html
3. https://onlinecourses.swayam2.ac.in/cec22_bt05/preview

Course Articulation Matrix

Programme Outcomes
Programme Specific Outcomes
Cognitive Level
Course
Outcomes
PO1 PO2 PO3 PO4 PO5 PO6 PO7 PO8 PSO1 PSO2 PSO3 PSO4 PSO5
K1
CO 1 2 3 3 3 3 2 2 2 3 3 2 2 3

K2
CO 2 2 3 3 3 3 2 2 2 3 3 3 2 3

K3
CO 3 2 3 3 3 3 2 2 2 3 3 3 2 3

K4
CO 4 2 3 3 3 3 2 2 2 3 3 3 2 -

K5
CO 5 2 3 3 3 3 2 2 2 3 3 2 2 3

Wt. Avg. 2 3 3 3 3 2 2 2 3 3 2.6 2 3

Wt. Avg. 2.5 2.7

Overall Mapping of the Course With PO 2.5

With PSO 2.7
 Semester IV – Core Practical
Microbial Genetics and Molecular Biology
Course Code

Credits 3
Hours / Cycle 4
Category Part Core Practical
Semester II
Year of From the academic year 2023 -2024
Implementation
1.To acquire hands-on training on molecular methods
2.To learn the isolation protocols of DNA and RNA
3.To understand and perform UV –mutagenesis and isolation of auxotrophic
Course Objectives mutants
4.To analyze the DNA and RNA by UV-Vis spectrometry and Agarose gel
electrophoresis.
5.To prepare competent cells for transformation experiment.

Bloom’s
PSO Taxonomy Levels
CO # Course Outcome(s)
Addressed (K1 to K5)

On completing the course successfully, the student will be able to

CO 1 Recall the procedures for the experiments in PSO1, PSO2, K1

microbial genetics and molecular biology PSO4 & PSO5
CO 2 Explain the resources used in experiments in the PSO1, PSO2, K2
practical courses. PSO4 &
PSO5
CO 3 Plan the experiments for isolation of DNA and RNA PSO1, PSO2, K3
from microorganisms, its identification, isolation of PSO4 &
mutants and preparation of competent cells. PSO5
CO 4 Analyse and interpret the results of the experiments PSO1, PSO2, K4
done. PSO4 &
PSO5
CO 5 Evaluate and explain the principle, method and the PSO1, PSO2, K5
outcome of the experiments in the practical course. PSO3, PSO4
& PSO5

SYLLABUS
UNIT CONTENT HOURS COs BLOOM’S
60 TAXONOMY
LEVEL
 I Isolation of plasmid-DNA by alkali-lysis method from 15 1,2,3,4,5 K1,2,3,4,5
E. coli
Isolation of Genomic DNA from E.coli
Isolation of RNA from yeast.
II Detection of plasmid DNA, genomic DNA and RNA 12 1,2,3,4,5 K1,2,3,4,5
by agarose gel electrophoresis.

III Preparation of competent cells by calcium chloride 9 1,2,3,4,5 K1,2,3,4,5

method.

IV Qualitative estimation of DNA and RNA by UV 12 1,2,3,4,5 K1,2,3,4,5

spectrophotometric method.
Quantitative estimation of RNA by Orcinol method.
Quantitative estimation of DNA by Diphenylamine
method
V Isolation of auxotrophic mutants by UV induced 12 1,2,3,4,5 K1,2,3,4,5
mutagenesis (Replica plating technique).
Isolation of antibiotic resistant mutants by gradient
plating method
Prescribed books/Text Books
1. Dubey R.C. & Maheshwari D.K. (2017). Practical Microbiology. S. Chand & Company Ltd.
2. Pranab Paul, Bhaskar Sarma. (2022). Molecular Biology with Practical, Mahaveer Publications
References
1.Suphiya khan, Swati Agarwal (2019) Advanced Lab Practices in Biochemistry & Molecular
Biology,Wiley Publications
2.Sue Carson, Heather Miller, Melissa Srougi, D. Scott Witherow (2019), Molecular Biology
Techniques - A Classroom Laboratory Manual, Academic press.
3.Gakhar S.K. et al.,(2021).Molecular Biology: A Laboratory Manual. Dreamtech Press
Suggested Reading
1. Masroor Ellahi Babar(2011).A Laboratory Manual of Molecular Biology. LAP Lambert
Academic Publishing
2.Chaitanya K.V. (2023). Cell and Molecular Biology. A Lab Manual.PHI Learning
Web Resources:
1.https://cals.arizona.edu/spls/sites/cals.arizona.edu.spls/files/428-
528%20Lab%20Manual%202019190326.pdf
2.https://uwosh.edu/biology/wp-content/uploads/sites/67/2018/04/Bio-377577-Kallas.pdf
3.https://www.cdc.gov/labtraining/training-courses/basic-molecular-biology/laboratorypractice.html
 Course Articulation Matrix

Programme Outcomes
Programme Specific Outcomes
Cognitive Level
Course
Outcomes
PO1 PO2 PO3 PO4 PO5 PO6 PO7 PO8 PSO1 PSO2 PSO3 PSO4 PSO5

CO 1 2 3 - 3 2 2 2 2 3 3 - 2 2 K1

CO 2 2 3 - 3 3 2 2 2 3 3 - 2 2 K2

CO 3 2 3 - 3 3 2 2 2 3 3 - 2 3 K3

CO 4 2 3 - 3 3 2 2 2 3 3 - 2 3 K4

CO 5 2 3 3 3 3 2 2 2 3 3 3 2 3 K5

Wt. Avg. 2 3 3 3 3 2 2 2 3 3 3 2 3

Wt. Avg. 2.5 2.8

Overall Mapping of the Course With PO 2.5

With PSO 2.8
 Semester IV– Allied Theory
Biotechnology
Course Code
Credits 4
Hours / Cycle 4
Category Part Allied Theory
Semester IV
Year of From the academic year 2023 -2024
Implementation
1.To understand the basic concepts of biotechnology
2.To learn the methods of Spirulina cultivation
3.To study the concept of genetically modified foods.
Course Objectives 4.To provide in depth knowledge in genetic engineering in plants and
animals
5.To learn the importance of Nanobiotechnology.

Bloom’s
Taxonomy
PSO
CO # Course Outcome(s) Levels
Addressed
(K1 to K5)

On completing the course successfully, the student will be able to

CO 1 Recall the fundamental knowledge in PSO1, PSO2, K1

Biotechnology PSO3, PSO5
CO 2 Explain the basic concepts in biotechnology PSO1, PSO2 K2
PSO3
PSO5
CO 3 Apply the biotechnological concepts in PSO1, PSO2, K3
pharmaceutical, agriculture and environment PSO3, PSO4
sectors. PSO5
CO 4 Analyze the prospects of plant, animal and PSO1, PSO2, K4
nanobiotechnology to society. PSO3, PSO4
PSO5
CO 5 Evaluate and formulate ideas to solve basic PSO1, PSO2, K5
problems and pursue a career in the field. PSO3, PSO4
& PSO5

SYLLABUS
UNIT CONTENT HOURS COs BLOOM’S
60 TAXONOMY
LEVEL
 I Introduction, History and Scope of Biotechnology. 12 1,2,3,4,5 K1,2,3,4,
Ethical issues in Biotechnology. Applications of 5
Biotechnology. Algal biotechnology - cultivation
methods of Spirulina - biotechnological potentials of
microalgae - food, feed, fuel - economically valuable
compounds of microalgae.

II Marine Biotechnology - Introduction: Significance of 12 1,2,3,4,5 K1,2,3,4,

marine natural products- Antibiotic, anti-tumour, 5
tumour-promotor, anti-inflammatory, analgesic,
cytotoxic, anti-viral, anti-fouling compounds of
marine origin. Biotechnological interventions for
combating marine pollution. Concept of blue
biotechnology and blue bioeconomy

III Food Biotechnology – Genetically modified foods 12 1,2,3,4,5 K1,2,3,4,

(GMF), Regulatory and social aspects of 5
biotechnology of foods. Pharmaceutical
biotechnology - concepts and industrial applications
- Biogeneric drugs – Vaccines, Hormones and
Therapeutic proteins.

IV Plant and Animal biotechnology: Plant 12 1,2,3,4,5 K1,2,3,4,

transformation methods, genetic engineering in 5
plants for virus resistance, pest resistance, delay of
fruit ripening, production of plantibodies.
Transformation of animal cells, invitro fertilization
and animal cloning.

V Introduction to Nanobiotechnology, Nanoparticle: 12 1,2,3,4,5 K1,2,3,4,

Applications in diagnostics and therapeutics, health 5
and disease, Environment, food and agriculture.
Nanotechnology in drug delivery.

Prescribed books/Text Books

1.K. Dass. (2005), Text book of Biotechnology, Second Edition, Wiley Dreamtech, India (P) Ltd.
2.P.K. Gupta. (2009) Elements of Biotechnology. Rastogi
3.H. D. Kumar. (2005) “Modern concepts of Biotechnology” Vikas publishing House. Jogdand S N.
(2008). Medical Biotechnology 2nd Edition Himalaya publishers
References
1.Heinrich Klefenz. (2002). Industrial pharmaceutical biotechnology
2.Kayser, O and Muller R.H. (2004). Pharmaceutical Biotechnology Drug Discovery and Clinical
Applications. Wiley-VCH
3.Smith, (2009). Biotechnology. Cambridge university Press.
Suggested Reading
1. Brown T. A. (2016). Gene Cloning and DNA Analysis: An Introduction. Wiley Blackwell. 7 th ed.
2. Primrose S.B. (2005). Principles of Gene Manipulation and Genomics. Wiley India. 7th ed.
3. Bhojwani, S.S and Razdan, M.K. (2010). Plant Tissue Culture: Theory and Practice Elsevier
Science
Web Resources
1.https://ihas.its.edu.in/Home/E-Resources-for-Biotechnology.aspx.
2. https://nabt.org/Resource-Links-Biotechnology-Genetics
3.https://crln.acrl.org/index.php/crlnews/article/view/19273/22457

Course Articulation Matrix

Programme Outcomes
Programme Specific Outcomes
Cognitive Level
Course
Outcomes
PO1 PO2 PO3 PO4 PO5 PO6 PO7 PO8 PSO1 PSO2 PSO3 PSO4 PSO5
K1
CO 1 2 3 3 3 3 2 2 2 3 3 3 - 3
K2
CO 2 2 3 3 3 3 2 2 2 3 3 3 - 2
K3
CO 3 2 3 3 3 3 2 2 2 3 3 3 2 2
K4
CO 4 2 3 3 3 3 2 2 2 3 3 3 2 3
K5, K6
CO 5 2 3 3 3 3 2 2 2 3 3 3 2 1

Wt. Avg. 2 3 3 3 3 2 2 2 3 3 3 2 2.2

Wt. Avg. 2.5 2.6

Overall Mapping of the Course With PO 2.5

With PSO 2.6
 Semester IV – Allied Practical
Biotechnology
Course Code
Credits 1
Hours / Cycle 2
Category Part Allied Practical
Semester IV
Year of From the academic year 2023 -2024
Implementation
1.To acquire hands-on training on biotechnological techniques
2.To learn the method of cultivation of spirulina and extraction of pigments.
3.To understand and perform plant explants preparation and culture
Course Objectives
4.To analyze the DNA isolated from plants. by gel-electrophoresis
5.To use immunodiagnostic, PCR and biochemical methods for disease
diagnosis.

Bloom’s Taxonomy
PSO Levels
CO # Course Outcome(s)
Addressed (K1 to K5)

On completing the course successfully, the student will be able to

CO 1 Recall the procedures for the experiments in PSO1, PSO2, K1

practical biotechnology. PSO3, PSO4
& PSO5
CO 2 Explain the resources used in the experiments of the PSO1, PSO2, K2
practical course. PSO3, PSO4
PSO5
CO 3 Plan the experiments for plant tissue culture, PSO1, PSO2, K3
cultivation of Chlorella, biosynthesis of PSO3, PSO4
nanoparticles and isolation of plant DNA. PSO5
CO 4 Analyse the results of the experiments performed. PSO1, PSO2, K4
PSO3, PSO4
PSO5
CO 5 Evaluate the principle, method and outcome of the PSO1, PSO2, K5
experiments, PSO3, PSO4
& PSO5

SYLLABUS
UNIT CONTENT HOURS COs BLOOM’S
30 TAXONOMY
LEVEL
I Cultivation of Chlorella 7 1,2,3,4,5 K1,2,3,4,5
Screening for antibiotics from marine algae.
 II Media preparation for plant tissue culture and 10 1,2,3,4,5 K1,2,3,4,5
explants culturing.
To demonstrate various steps of explant inoculation.
III Genomic DNA isolation from plants. 5 1,2,3,4,5 K1,2,3,4,5

IV Biosynthesis of nanoparticles and characterization 5 1,2,3,4,5 K1,2,3,4,5

using UV spectroscopy

V Demonstration of PCR 3 1,2,3,4,5 K1,2,3,4,5

Prescribed books/Text Books:

1.KR. Aneja. (2001). Experiments in Plant pathology, Tissue culture and mushroom production technology.
New Age International (p) Ltd. Publishers.
References
1.DBT Life Sciences Protocol Manual January (2018).
2.Ashish S.Verma (2014). Laboratory manual for biotechnology. S.Chand Publication
3.Pavan J.Kundur.(2017). Laboratory manual for biotechnology. PC Jabin Science College
Suggested Reading
1.Harisha S. (2007). Biotechnology procedures and experiments handbook. INFINITY SCIENCE PRESS
LLC.
2. Purohit S,S. (2018). Laboratory Manual of Plant Biotechnology. 2nd Ed.IBP.
Web Resources
1.https://www.austincc.edu/awheeler/Files/BIOL%201414%20Fall%202011/BIOL1414_Lab%20Man
ual_Fall%202011.pdf
2. https://www.rose-hulman.edu/~brandt/publications/472B_Lab_Manual_2ndEd.pdf
3 https://webstor.srmist.edu.in/web_assets/downloads/2021/18BTC108J-lab-manual.pdf
4. https://indiabioscience.org/media/articles/DBT-Life-Science-Protocol-Manual.pdf

Course Articulation Matrix

Programme Outcomes
Programme Specific Outcomes
Cognitive Level
Course
Outcomes
PO1 PO2 PO3 PO4 PO5 PO6 PO7 PO8 PSO1 PSO2 PSO3 PSO4 PSO5
K1
CO 1 2 3 3 3 3 2 2 2 3 3 3 2 3
K2
CO 2 2 3 3 3 3 2 2 2 3 3 3 2 2
K3
CO 3 2 3 3 3 3 2 2 2 3 3 3 2 2
K4
CO 4 2 3 3 3 3 2 2 2 3 3 3 2 3
K5
CO 5 2 3 3 3 3 2 2 2 3 3 3 2 2

Wt. Avg. 2 3 3 3 3 2 2 2 3 3 3 2 2.4

Wt. Avg. 2.5 2.7

Overall Mapping of the Course With PO 2.5

With PSO 2.7
 Semester V – Core Theory
Bacteriology and Virology
Course Code
Credits 5
Hours / Cycle 5
Category Part Core Theory
Semester V
Year of From the academic year 2023-2024
Implementation
1.To acquire knowledge about important Gram-positive bacterial pathogens.
2.To understand about Gram negative bacterial pathogens.
Course Objectives 3.To learn about Spirochaetes and Rickettsia diseases.
4.To learn about basics of viral structure, pathogenesis, diagnosis of viral
diseases.
5.To study about emerging and re-emerging viral diseases

Bloom’s Taxonomy
PSO Levels
CO # Course Outcome(s)
Addressed (K1 to K5)

On completing the course successfully, the student will be able to

CO 1 Recall the knowledge on clinically important PSO1, PSO2, K1

bacteria and viruses
CO 2 Demonstrate the mechanisms of bacterial and viral PSO1, PSO2, K2
pathogenesis for diagnosis and treatment PSO4

CO 3 Apply various laboratory methodologies for PSO1, PSO2, K3

diagnosis of bacterial and viral diseases. PSO4,
PSO5,
CO 4 Discover proper isolation procedures for pathogens PSO1, PSO2, K4
from clinical samples. PSO4,
PSO5
CO 5 Prioritize the knowledge on bacterial and viral PSO1, PSO2, K5
diseases. PSO3,
PSO4, PSO5

SYLLABUS
UNIT CONTENT HOURS COs BLOOM’S
75 TAXONOMY
LEVEL
 I Normal microflora of the human body and its 15 1,2,3,4, K1,2,3,4,5
importance. Classification, morphology, culture 5
identification, pathogenesis, laboratory diagnosis and
prophylaxis of Gram-positive bacterial pathogens -
Staphylococcus, Streptococcus, Corynebacterium,
Clostridium, Bacillus, Mycobacterium tuberculosis.
Antimicrobial chemotherapy-Antibiotics, types and
mode of action/resistance

II Nosocomial infection. Classification, morphology, 15 1,2,3,4, K1,2,3,4,5

culture identification, pathogenesis, laboratory 5
diagnosis and prophylaxis of Gram -negative
bacterial pathogens – Neisseria, Salmonella,
Shigella, Escherichia coli, Pseudomonas, Vibrio.
III Introduction to Rickettsia (typhus fever group and 15 1,2,3,4, K1,2,3,4,5
Rocky Mountain spotted fever) Spirochetes 5
Treponema and Leptospira. Antimicrobial
susceptibility tests.

IV General properties of viruses and its common 15 1,2,3,4, K1,2,3,4,5

diagnostic methods. Brief account on classification, 5
morphology, cultivation, antigenic structure,
pathogenesis, laboratory diagnosis and prophylaxis of
the following group of viruses – Variola (Pox virus),
Herpes simplex virus, Polio viruses, HIV, Hepatitis A
and B virus, Rabies virus, Mumps virus and Dengue
virus.

V Introduction to emerging and re-emerging viruses - 15 1,2,3,4, K1,2,3,4,5

H1N1 Flu (Swine Flu), Ebola and Chikungunya, 5
SARS-CoV-2 (Coronavirus)

Text books:
1.Satish Gupte. (2018). The short textbook of Medical Microbiology. 8 th Edition, Jaypee Brothers,
Medical Publishers (P) Ltd., New Delhi.
2.Allan.J. Cann (2015). Principles of molecular virology.6 th edition by academic press, an imprint of
Elsevier publishers.
3.Bailey & Scott (2021). Diagnostic microbiology. 15th edition Elsevier publishers.
References:
1.Gerhardt, P., Murray, R.G., Wood, W.A. and Kreig, N.R. (Eds) (1994) Methods for General and
Molecular Bacteriology. ASM Press, Washington, DC.
2.Tom Parker,M., Leslie H. Collier. (1990). Topley &Wilson’s Principles of Bacteriology,Virology and
Immunity (8th Edition).London : Edward Arnold.
3.Greenwood, D., Slack, R.B. and Peutherer, J.F. (2012) Medical Microbiology, 18 th Edn. Churchill
Livingstone, London
4.Flint SJ, Enquist LW, King RM, Racaniello VR and Shalka AM (2000). Principles of Virology and
Molecular Biology, pathogenesis and control, ASM Press, Washington DC.
5. P.Saravanan. (2006). Virology, MJP Publishers, Chennai.
Suggested Reading:
1.Ananthanarayanan R and Jayaram Panikaer, C.K. (2017). 10th edition. Textbook of Microbiology,
Orient Longman.
2.Salle. A.J. (2007). Fundamental principles of bacteriology, 4th edition, Tata McGraw-Hill
Publications.
3.Edward K. Wagner, Martinez J. Hewlett, (2004). Basic Virology, Blackwell Publishing
Web Resources:
1.https://www.cartercenter.org/
2.http://users.ox.ac.uk/-genemed/virology.htm
3.http://virology-online.com/

Course Articulation Matrix

Programme Outcomes
Programme Specific Outcomes
Cognitive Level
Course
Outcomes
PO1 PO2 PO3 PO4 PO5 PO6 PO7 PO8 PSO1 PSO2 PSO3 PSO4 PSO5
K1
CO 1 2 3 - 3 3 3 3 - 3 3 3 - -
K2
CO 2 2 2 - 3 3 3 3 2 - - 2 3 -
K3
CO 3 3 2 - 3 3 3 3 3 - 2 - 3 2
K4
CO 4 3 3 - 3 2 2 3 2 - - 3 3 2
K5
CO 5 3 2 2 2 2 2 2 2 - 3 2 3 2

Wt. Avg. 2.6 2.4 2 2.8 2.6 2.6 2.8 2.3 3 2.6 2.5 3 2

2.5 2.6

Overall Mapping of the Course With PO 2.5

With PSO 2.6
 Semester V – Core Practical
Bacteriology and Virology
Course Code
Credits 2
Hours / Cycle 3
Category Part Core Theory
Semester V
Year of Implementation From the academic year 2023-2024

1.To learn about different staining techniques in the identification of bacteria.

2.To acquire skill on isolation and identification of pathogenic bacteria by
cultural and biochemical tests.
Course Objectives
3.To study antibacterial sensitivity methods.
4.To understand the minimum inhibitory concentration test for bacteria.
5.To know about viral inclusion bodies.

Course Outcome BLOOM’S

CO No. (Upon completion of this course, students will PSOs Addressed TAXONOMY
be able to) LEVEL
List the procedures of practical experiments PSO1, PSO2,
CO1 related to bacteriology and virology. K1

Explain about the materials, equipment and

K2
CO2 other resources used in isolation of pathogenic PSO1, PSO2,
bacteria and viruses.
Apply the diagnostic methods in bacterial and PSO1, PSO2, PSO3,
CO3 viral diseases. K3
PSO4
CO4 Analyse the significance of each of the PSO1, PSO2, PSO5 K4
procedures used.
Recommend isolation procedures and tests for PSO1, PSO2, PSO3,
CO5 diagnosis of bacterial and viral diseases. K5
PSO4, PSO5

SYLLABUS
UNIT CONTENT HOURS COs BLOOM’S
45 TAXONOMY
LEVEL
I Direct microscopy of clinical specimens - Throat 10 1,2,3, K1,2,3,4,5
swab, Pus, Sputum and Stool. 4,5
II Isolation and identification of bacterial pathogens 13 1,2,3,4 K1,2,3,4,5
from the following samples by culturing. ,5
a. Sputum
b. Pus
c. Urine
d. Faeces
e. Blood
 III Identification of bacterial pathogens by biochemical 8 1,2,3,4 K1,2,3,4,5
tests ,5
IV Antimicrobial susceptibility test by Kirby-Bauer disc 7 1,2,3,4 K1,2,3,4,5
diffusion method. ,5
V Minimum inhibitory concentration (MIC) test- 7 1,2,3,4 K1,2,3,4,5
Demonstration. Spotters –Virology ,5

Text books:
1.D. Roy Cullimore (2010). Practical Atlas for Bacterial Identification, 2nd edition.
Publisher-Taylor &Francis.
2.Alexander Crever Abbott, (2010). The Principles of Bacteriology, Nabu Press.
Reference Books:
1.Henrik Chart, (2018). Practical Laboratory Bacteriology. CRC Press.
2.Veranus A Moore, (2017). Laboratory Directions for Beginners in Bacteriology. Triste
Publishing Ltd.
3.Mackie & McCartney, (2008). Practical Medical Microbiology 14 th edition. Elsevier
Publishers.
Suggested Reading:
1.Abbot A.C,(2018).The Principles of Bacteriology: A Practical Manual for Students and
Physicians. Forgotten Books Publishing.
2.Florence G. Burleson, Thomas M. Chambers, Danny L. Wied Brauk, (2014). Virology –
A Laboratory Manual, Academic Press.

Web Resources:
1.https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3768729/
2.https://www.researchgate.net/
3.https://bio.libretexts.org/

Course Articulation Matrix

Programme Outcomes
Programme Specific Outcomes
Cognitive Level
Course
Outcomes
PO1 PO2 PO3 PO4 PO5 PO6 PO7 PO8 PSO1 PSO2 PSO3 PSO4 PSO5
K1
CO 1 1 3 - 2 3 3 3 - 3 3 - - -
K2
CO 2 2 2 - 2 2 3 3 - 3 3 - - -
K3
CO 3 3 2 3 2 2 3 3 - 3 2 3 3 -
K4
CO 4 2 3 - 3 3 2 3 2 3 2 - - 3
K5
CO 5 2 2 3 3 2 2 3 2 3 3 3 3 3

Wt. Avg. 2 2.4 3 2.4 2.4 2.6 3 2 3 2.6 3 3 3

2.4 2.9

Overall Mapping of the Course With PO 2.4

With PO 2.9
 Semester V - Core Theory
Industrial Microbiology
Course Code
Credits 5
Hours / Cycle 5
Category Part Core Theory
Semester V
Year of From the academic year 2023-2024
Implementation
1. To study the role of commercially-potent microorganisms in industrial
processes.
2. To learn about the industrial fermentation processes.
Course Objectives 3. To know about the production of important vitamins and beverages and
their recovery.
4. To have basic knowledge on IPR for biotechnological products.
5. To understand the role of a microbiologist in the fermentation industry.
Bloom’s
Taxonomy
PSO
CO # Course Outcome(s) Levels
Addressed
(K1 to K5)

On completing the course successfully, the student will be able to

CO 1 Relate the fundamental knowledge on various aspects PSO1, PSO2, K1

of the fermentation processes PSO3
CO 2 Explain in detail the nuances of industrial PSO1, PSO2 K2
fermentation PSO5
CO 3 Apply the knowledge of fermentation for PSO1, PSO2 K3
commercial purposes. PSO3, PSO5
CO 4 Examine in detail the fermenters and their operation, PSO1, PSO2 K4
and fermentation processes for microbial products PSO3, PSO5
CO 5 Determine the prospects in industrial fermentation. PSO1, PSO2, K5
PSO3, PSO4
& PSO5

SYLLABUS
UNIT CONTENT HOURS COs BLOOM’S
75 TAXONOMY
LEVEL
I Introduction, history and development – Prospecting 15 1,2,3,4 K1,2,3,4,5
and screening of commercially important ,5
 microorganisms – Concept of strain improvement
and its techniques – Culturing and maintenance of
industrially important microbes - Development of
inoculum for various fermentation processes.
Concepts of Immobilization.
II Fermenter: principles, components, design and types 15 1,2,3,4 K1,2,3,4,5
– Types of fermentation: Submerged and solid state; ,5
Batch, fed-batch, Continuous and semi-continuous
culture systems – Operation, optimization and
automation of fermentation processes. Computer
applications in fermentation technology.
III Upstream processing: Scaling up of the culture - 15 1,2,3,4 K1,2,3,4,5
Commercial media used in bacterial and fungal ,5
fermentation – Industrial sterilization: fermenters,
fermenter components and media. Downstream
processing: Cell separation, cell lysis and product
recovery. Purification by different methods. Drying
and crystallization.
IV Microbial production of amylase – Production of L- 15 1,2,3,4 K1,2,3,4,5
Glutamic acid – Acetic acid and citric acid ,5
production – Vitamin B12 production from
microorganisms - Manufacture of alcoholic
beverages by fermentation: beer and wine.
Recombinant Products - insulin, interferon and
growth hormone. Single cell proteins: bacterial,
fungal and algal SCPs.
V Microbial production of biosurfactants, bioplastics 15 1,2,3,4 K1,2,3,4,5
and other biopolymers (xanthan and cellulose) - ,5
Brief account of patenting processes in India.
Government initiatives: Technology business
incubators, SBIRI.
Prescribed Books/Textbooks
1. Patel A.H. (2016). Industrial Microbiology. 2nd edition. Laxmi Publications.
2. Reed G. (2004). Prescott and Dunn’s Industrial Microbiology, Macmillan.
3. Casida L.E. (1968). Industrial Microbiology. Wiley.
References
1.Waites M.J., Morgan N.L., Rockey J.S. & Higton G. (2013). Industrial Microbiology – An
Introduction. Blackwell Science.
2.Stanbury P.F., Whitaker A., Hall S.J. (2016). Principles of Fermentation Technology
3rd edition. Butterworth-Heinemann, Elsevier.
3.Fogarty W.M. & Kelly C.T. (2012). Microbial Enzymes and Biotechnology 2 nd edition.
Elsevier Applied Science.
4.Goldberg E. (2012). Handbook of Downstream Processing. Blackie Academic &
professional, Chapman and Hall.
5.Singh K.K. (2014). Biotechnology and Intellectual Property Rights: Legal and Social
Implications. Springer.
Suggested Reading
1.Sivakumar P.K., Joe M.M and Sukesh K. (2010). An introduction to Industrial Microbiology. 1 st edn. S.
Chand and Company Ltd, New Delhi.
2.Chandrakant K. (2016). Pharmaceutical Microbiology. 1st edition, Nirali Publication.
3.Peppler H.J, and Periman D. (1979) Fermentation Technology, Vol 1 & 2, 2nd edn. Academic Press, London
Web Resources
1.https://nptel.ac.in/courses/102105058
2.Food and Industrial Microbiology: Tutoring Solution - Videos & Lessons | Study.com
3.https://nptel.ac.in/courses/102105058

Course Articulation Matrix

Programme Outcomes
Programme Specific Outcomes
Cognitive Level
Course
Outcomes
PO1 PO2 PO3 PO4 PO5 PO6 PO7 PO8 PSO1 PSO2 PSO3 PSO4 PSO5
K1
CO 1 2 3 3 2 2 3 2 - 3 3 2 - -

K2
CO 2 2 3 - 2 3 3 3 3 3 3 - - 3

K3
CO 3 2 3 3 2 3 3 3 3 3 3 3 - 3

K4
CO 4 2 3 3 2 3 3 2 3 3 3 3 - 3
K5
CO 5 2 3 3 2 2 3 3 3 3 3 3 3 3

Wt. Avg. 2 3 3 2 2.6 3 2.6 3 3 3 2.8 3 3

Wt. Avg. 2.7 3

Overall Mapping of the Course With PO 2.7

With PSO 3
 Semester V - Core Theory
Environmental Microbiology
Course Code
Credits 5
Hours / Cycle 5
Category Part Core Theory
Semester V
Year of
From the academic year 2023-24
Implementation

1.To know the basic and advanced concepts of environmental microbiology

2. To relate and appreciate the significance of microorganisms in the field of
Course Objectives applied environmental microbiology
3.To identify the recent trends with respect to the fields of air, water and soil
microbiology.
4.To know about infectious crop diseases and their management.
5.To perceive the importance of use of biofertilizers and biopesticides.
Bloom’s
PSO Taxonomy Levels
CO # Course Outcome(s)
Addressed (K1 to K5)

On completing the course successfully, the student will be able to

PSO1, PSO2,
Recall about the fundamentals of environmental
CO 1 PSO3, PSO4, K1
microbiology and its application aspects
PSO5
Explain the concepts in detail related to different PSO1, PSO2,
CO 2 domains of environmental microbiology PSO3, PSO4, K2
PSO5
Choose and apply the sampling protocols with respect PSO1, PSO2,
CO 3 to different environments PSO4, PSO5 K3

Analyse the pertinent problems and issues through PSO1, PSO2,

CO 4 understanding the pros and cons of developments in PSO4, PSO5 K4
environmental microbiology
PSO1, PSO2,
Appraise and prioritise the significant issues in the
CO 5 PSO3, PSO4, K5
field of environmental microbiology
PSO5

SYLLABUS

HOURS BLOOM’S
UNIT CONTENT COs
75 TAXONOMY
LEVEL
 I Soil characteristics, soil profile, soil as a natural 13 1,2,3, K1,2,3,4,5
habitat of microbes, soil microflora –Aquatic 4,5
environment: stratification, nutrient availability and
microflora of fresh water, marine habitats –
Extremophiles: microbes thriving at high and low
temperatures, pH, high hydrostatic and osmotic
pressures, hypersaline environments – Concept of
quorum sensing.

II Microbiology of water and water pollution – water- 18 1,2,3, K1,2,3,4,5

borne diseases, their prevention and control – 4,5
Microbiological examination of water and indicator
organisms – Bioremediation, treatment of domestic
and industrial wastewaters: physical, chemical and
biological treatments, sludge processing – TNPCB
norms and regulations for discharge water.
III Microbiology of air: Bioaerosols and aeroallergens – 13 1,2,3, K1,2,3,4,5
Factors influencing the microbial load in air – Air 4,5
sampling devices and enumeration of air microflora
– Air-borne diseases - Air sanitation and
microbiological air standards – Control of
bioaerosols

IV Biogeochemical cycles – carbon, iron, phosphorus 13 1,2,3, K1,2,3,4,5

and sulphur cycles – Nitrogen fixation-symbiotic and 4,5
nonsymbiotic – Microbial degradation of
polysaccharides [cellulose, hemicellulose, lignin]
and Humus formation – Production of biofuels:
biodiesel, bioethanol and biomethane.

V Brief account of bacterial, fungal and viral plant 18 1,2,3, K1,2,3,4,5

diseases – Microbial biopesticides: Advantages and 4,5
drawbacks – Bacterial and cyanobacterial
biofertilizers and their mass production (Rhizobium,
Azotobacter): standards and quality control and field
application methods– Integrated pest management
system.

Prescribed Books/Textbooks
1.Sharma P.D. (2019). Microbiology and Plant pathology (2nd edition). Rastogi Publications.
2.Mishra A. (2011). Plant Pathology: Diseases and Management, Agrobios, India.
3.Okafor, N (2011). Environmental Microbiology of Aquatic & Waste systems. 1st edition, Springer,
New York.
4.Barton L.L. & Northup D.E. (2011). Microbial Ecology, Wiley-Blackwell.
 References
1.Pepper I.L., Gerba C.P. & Gentry T.J. (2014). Environmental Microbiology.3rd edition. Academic
Press, Elsevier.
2.Bitton G. (2011). Wastewater Microbiology. 4th edition. Wiley-Blackwell.
3.Bridgewater L. (2012). Standard Methods for the Examination of Water and Wastewater. American
Public Health Association.
4.Woolverton C.J., Sherwood L., Willey J. (2016). Prescott’s Microbiology. 10th edition. McGraw-Hill
Education.
5.Tinsley S., & Pillai I. (2012). Environmental Management Systems – Understanding Organizational
Drivers and Barriers. Earthscan.

Suggested Reading
1. Ji-Dong Gu, Ralph Mitchell (2010). Environmental Microbiology (Edited Book). Wiley.
2. Bibhuti B. M., Suraja K. N., Swati M. (2021). Environmental and Agricultural Microbiology:
Applications for Sustainability (Edited Book). Wiley.

Web Resources
1. https://onlinecourses.nptel.ac.in/noc21_ce07/preview
2. https://onlinecourses.nptel.ac.in/noc19_ce32/preview
3. https://onlinecourses.nptel.ac.in/noc19_bt16/preview

Course Articulation Matrix

Programme Outcomes
Programme Specific Outcomes
Cognitive Level
Course
Outcomes
PO1 PO2 PO3 PO4 PO5 PO6 PO7 PO8 PSO1 PSO2 PSO3 PSO4 PSO5
K1
CO 1 3 3 3 2 3 2 2 3 3 3 3 3 2
K2
CO 2 3 3 3 1 3 2 2 3 3 2 3 3 2
K3
CO 3 1 3 - 1 3 2 2 3 3 3 - 3 2
K4
CO 4 2 3 - 1 2 2 2 3 3 2 - 3 3
K5
CO 5 3 3 3 2 3 2 2 3 3 3 3 3 2

Wt. Avg. 2.4 3 3 1.4 2.8 2 2 3 3 2.6 3 3 2.2

Wt. Avg. 2.5 2.8

Overall Mapping of the Course With PO 2.5

With PSO 2.8
 Semester V - Core Practical
Industrial and Environmental Microbiology
Course Code
Credits 4
Hours / Cycle 6
Category Part Core Practical
Semester V
Year of
From the academic year 2023-24
Implementation

1.To know about isolation and maintenance of commercially important

microorganisms
Course Objectives 2.To understand the fermentation process at the laboratory level
3.To learn about environmental sampling and transport techniques
4.To develop skills in performing microbiological analysis of environmental
samples
5.To identify plant pathogens based on culture and microscopic methods
Bloom’s
PSO Taxonomy Levels
CO # Course Outcome(s)
Addressed (K1 to K5)

On completing the course successfully, the student will be able to

List, recall and tell the procedures of practical

CO 1 experiments relating to industrial and environmental PSO1, PSO2, K1
microbiology PSO4, PSO5

Explain about the materials, equipment and other PSO1, PSO2,

CO 2 resources used in industrial and environmental PSO4, PSO5 K2
microbiology practical experiments
Plan for experiments pertaining to isolation of PSO1, PSO2,
commercially important microorganisms, PSO4, PSO5
CO 3 K3
fermentation processes, and microbiological analysis
of environmental samples
Analyse the outcome of the industrial and PSO1, PSO2,
CO 4 environmental microbiology experiments and relate it PSO4, PSO5 K4
to statutory standards
Evaluate and explain methods, principles and PSO1, PSO2,
CO 5 outcomes of industrial and environmental PSO3, PSO4, K5
microbiology experiments PSO5

SYLLABUS
 HOURS BLOOM’S
UNIT CONTENT COs
90 TAXONOMY
LEVEL
I Industrial Microbiology 20 1,2,3,4,5 K1,2,3,4,5
Preparation of wine from grape juice and
estimation of alcohol.
Preparation of immobilized yeast cells.
Production of citric acid using Aspergillus niger.
Screening of antibiotic-producing organisms
from soil.
II Isolation of organisms producing amylases, 20 1,2,3,4,5 K1,2,3,4,5
cellulases and proteases from soil.
Production of cellulase by solid-state
fermentation.
Cultivation of Spirulina
III Environmental Microbiology 18 1,2,3,4,5 K1,2,3,4,5
Sample collection protocols – soil, air and water.
Enumeration of microorganisms from soil.
Enumeration of microorganisms from water.
Assessment of microbiological quality of air by
settle plate method, active air sampling method
and surface impingement method
IV Isolation of Rhizobium from root nodules of 14 1,2,3,4,5 K1,2,3,4,5
leguminous plants.
Isolation of phosphate-solubilizing
microorganisms from soil.
Study of plant pathogens – Erwinia,
Pseudomonas, Fusarium, Alternaria.
V Qualitative checking of coliforms by Presence 18 1,2,3,4,5 K1,2,3,4,5
Absence test.
Determination of coliforms in water by MPN
method.
Detection of faecal coliforms in water by
Eijkman’s test.
Kinetics of disinfection of water using
hypochlorite and checking the disinfection
efficiency.

Prescribed Books/Textbooks
1.Baltz, Richard H.; Davies, Julian E.; Demain, Arnold L. (2010). Manual of Industrial Microbiology
and Biotechnology (3rd Edition). American Society for Microbiology (ASM).
2.K.R. Aneja. (2018). Laboratory Manual of Microbiology and Biotechnology.
3.Gunasekaran P. (2007). Laboratory Manual in Microbiology. New Age International.
4.Hurst, C.J., Crawford R.L., Garland J.L., Lipson D.A., Mills A.L., Stetzenbach L.D. (2007). Manual
of Environmental Microbiology. 3rd Edition. American Society for Microbiology
 References
1.S. Kulandaivel, S. Janarthanan (2012). Practical Manual on Fermentation Technology. I K
International Publishing House Pvt. Ltd.
2.Pepper I., Gerba C., Brendecke J. (2004). Environmental Microbiology - A Laboratory Manual 2nd
Edition. Academic Press, Elsevier.
3.Yates M.V., Nakatsu C.H., Miller R.V., Pillai, S.D. (2016). Manual of Environmental Microbiology
4th Edition. Wiley.
Suggested Reading
1.Brian McNeil & Linda M. Harvey (2008). Practical Fermentation Technology. John Wiley & Sons,
Ltd.
2.Cindy H. Nakatsu, Robert V. Miller, Suresh D. Pillai (2020). Manual of Environmental
Microbiology, Fourth edition. Wiley.
3.John F. T. Spencer, Alicia L. Ragout de Spencer (2004). Environmental Microbiology: Methods
and Protocols. Humana Press.
Web Resources
1. https://onlinelibrary.wiley.com/doi/book/10.1128/9781555816827
2.https://vlab.amrita.edu/index.php?sub=3&brch=272
3.https://nptel.ac.in/courses/102105087

Course Articulation Matrix

Programme Outcomes
Programme Specific Outcomes
Cognitive Level
Course
Outcomes
PO1 PO2 PO3 PO4 PO5 PO6 PO7 PO8 PSO1 PSO2 PSO3 PSO4 PSO5
K1
CO 1 3 3 - 1 3 3 1 3 3 3 - 3 3
K2
CO 2 3 3 - 1 3 3 1 3 3 3 - 3 3
K3
CO 3 2 3 - 2 3 2 1 3 3 3 - 3 2
K4
CO 4 2 3 - 2 3 2 1 3 3 3 - 3 2
K5
CO 5 3 3 3 3 3 3 2 3 3 3 3 3 3

Wt. Avg. 2.6 3 3 1.8 3 2.6 1.2 3 3 3 3 3 2.6

Wt. Avg. 2.5 2.9

Overall Mapping of the Course With PO 2.5

With PSO 2.9
 Semester V – Core Theory
Computer Training
Course Code
Credits 3
Hours / Cycle 2
Category Part Core Theory
Semester V
Year of From the academic year 2023-24 onwards
Implementation
1.To know about the basics of computers.
2.To have an insight in various computing platforms.
Course Objectives 3.To learn about HTML function and Web Page designing.
4.To acquire knowledge about networking basics.
5.To retrieve the data from biological databases.
Bloom’s
Taxonomy
PSO
CO # Course Outcome(s) Levels
Addressed
(K1 to K5)

On completing the course successfully, the student will be able to

CO 1 Define the fundamental concepts of computers and PSO1, PSO2, K1

bioinformatics. PSO3, PSO4
PSO5
CO 2 PSO1, PSO3, K2
Demonstrate the basic and common tools PSO4, PSO5
used for working with computers and bioinformatics.

CO 3 Develop skills to use a computer, internet, web page PSO1, PSO3, K3

and bioinformatics software. PSO5
CO 4 Discover knowledge of computer applications and K4
bioinformatics software tools in all the fields of PSO1, PSO3,
higher learning and research. PSO4, PSO5

CO 5 Evaluate the importance of computers and PSO1, PSO2, K5

bioinformatics database in life sciences by providing PSO3, PSO5
support in computational biology

SYLLABUS
UNIT CONTENT HOURS COs BLOOM’S
30 TAXONOMY
LEVEL
I Introduction and history of Computers, Anatomy 05 1,2,3,4, K1,2,3,4,5
of a Computers, Types of computers, Input and 5
output devices, hardware and software.
 II Computing Platform - Windows, Macintosh, Unix, 06 1,2,3,4, K1,2,3,4,5
Linux. Computers for documentation, Processing 5
and Presentation - MS Word, Database and graph
generation: MS –Excel, project presentation: MS-
Power point. Use of graph pad prism
III Basics of WEB Designing: Basic HTML, Text 06 1,2,3,4, K1,2,3,4,5
styles, Adding pictures, backgrounds and music, 5
tables and frames.
IV Computer communication: Introduction to internet 06 1,2,3,4, K1,2,3,4,5
web browsing, E-mail, Search engines, 5
Networking, finding scientific articles – PubMed,
Medline. Application of Computers in Medicine
and Research
V Introduction to bioinformatics, Applications of 07 1,2,3,4, K1,2,3,4,5
bioinformatics, database and its types, Nucleic 5
acid databases (NCBI, DDBJ, and EMBL).
Protein databases (Primary, Composite and
Secondary). Specialized
Genome database (SGD, TIGR). Structure
databases (CATH, SCOP), finding and retrieving
sequences.
Prescribed Books/Textbooks
1.Norton P. (2008). Introduction to Computer, 6th edn. Tata McGraw Hill Pulications.
2.Xiong J, (2011). Essential Bioinformatics, 1st Edition, Cambridge University Press.
3.Rastogi S.C., Mendiratla N. and Rastogi P. (2013). Bioinformatics Methods and Applications-
Genomics, Proteomics and Drug Discovery. Prentice Hall India.
References
1.Rajaraman V. (2003). Fundamentals of Computers, 4th edn. PHI.
2.Mount D.W., (2013). Bioinformatics sequence and genome analysis, 2nd edn. CBS Publishers, New
Delhi.
3.Baxevanis A.D. Francis Ouellette B. F. (2012) Bioinformatics: A Practical Guide to the Analysis of
Genes and Proteins. 3rd edn. Wiley and Sons.
Suggested Reading
1.Niederst Robbins. (2018). Learning Web design. A Beginner’s guide for HTML.
2.Woody Leonhard. (2015). Windows 10 All in one for Dummies. Wiley.
3.Attwood T.K. and Parry-Smith D.J. (1999). Introduction to Bioinformatics. Addision Wesley
Longman Limited, England.
Web Resources
1. www.intel.in
2. http://www.buzzle.com/articles/roleof-computers-in-research.html
3. http://richmccue.com.
4.http://computersight.com/computers/therole-of-computer-in-research/
5 http://www.blurtit.com/
 Course Articulation Matrix

Programme Outcomes
Programme Specific Outcomes
Cognitive Level
Course
Outcomes
PO1 PO2 PO3 PO4 PO5 PO6 PO7 PO8 PSO1 PSO2 PSO3 PSO4 PSO5
K1
CO 1 2 3 3 3 3 3 3 3 3 3 3 3 3

K2
CO 2 2 3 3 3 3 3 3 3 3 - 3 3 3

K3
CO 3 2 3 3 - - - 3 3 3 - - - 3

K4
CO 4 2 3 3 3 3 - 3 3 3 - - 3 3

K5
CO 5 2 3 - 3 3 3 3 - 3 3 3 3 3

Wt. Avg. 2 3 3 3 3 3 3 3 3 3 3 3 3

Wt. Avg. 2.8 3

Overall Mapping of the Course With PO 2.8

With PSO 3
 Semester V – General Elective
Bioenergy from waste resources
Course Code
Credits 3
Hours / Cycle 4
Category Part General Elective Theory
Semester V
Year of
From the academic year 2023-24
Implementation
1.To know about the importance of waste management
2.To relate the use of various biomaterials for bioenergy applications
3.To know various types of biofuels that can be generated from waste
Course Objectives resources
4.To analyse the environmental benefits of CO2 biosequestration
5.To evaluate the advantages of biofuels in comparison with non-
renewable energy
Bloom’s
Taxonomy
PSO
CO # Course Outcome(s) Levels
Addressed
(K1 to K5)

On completing the course successfully, the student will be able to

Relate various biological waste management PSO1, PSO3,

CO 1 strategies and bioenergy production for improved PSO4 K1
sustainability
PSO1, PSO3,
CO 2 Summarize various bioenergy production processes PSO4 K2

Choose various wastes as resources for conversion PSO1, PSO3,

CO 3 into bioenergy PSO4 K3

Compare the production processes of various PSO1, PSO3,

CO 4 biofuels and their sustainability levels PSO4 K4

Prioritize the significance of producing bioenergy PSO1, PSO3,

CO 5 from wastes PSO4, PSO5 K5

SYLLABUS
BLOOM’S
HOURS TAXONOMY
UNIT CONTENT COs
60 LEVEL
 I Renewable energy for the 21st century – Bioenergy 12 1,2,3, K1,2,3,4,5
and its significance, Environmental benefits of 4,5
bioenergy utilization – sustainability and carbon
emissions. Zero-waste/waste-to-wealth approach -
circular bioeconomy – Bioenergy value chain –
National policy on biofuels.

II Biosolids generation from domestic wastewater 12 1,2,3, K1,2,3,4,5

treatment – Biomethane production from biosolids – 4,5
role of methanogens and the process of biological
methane production. Organic solid wastes from
industries and vegetable waste as substrates for
biomethane production.

III Introduction to microbial fuel cells (MFCs) and 12 1,2,3, K1,2,3,4,5

microbial electrolysis cells. MFCs for wastewater 4,5
treatment. Hydrogen production from waste
materials using microorganisms – dark and photo
fermentation.

IV Bioremediation of sewage and industrial effluents 12 1,2,3, K1,2,3,4,5

using microalgae, biocapture of atmospheric and 4,5
industrial CO2 by the above process – microalgae
biomass as feedstock for biofuels. Production of
biodiesel, biocrude and syngas from microalgae
biomass.

V Bioethanol and biobutanol as alternative fuels. 12 1,2,3, K1,2,3,4,5

Degradable wastes – agricultural and agroforestry 4,5
wastes, and molasses for bioethanol production.
Pretreatment processes, and process of yeast-
mediated bioethanol production and distillation.
Prescribed Books/Textbooks
1. Anju Dahiya (2020). Bioenergy: Biomass to biofuels and waste to energy. Academic Press.
2. Yebo Li, Samir Kumar Khanal (2017). Bioenergy – principles and applications. Wiley.
3. Marc Rogoff, Francois Screve (2019). Waste-to-Energy, 3rd edition. Elsevier.

References
1.Chaudhery M. H., Sunpreet Singh, Lalit Goswami (2022). Waste-to-energy approaches towards zero
waste. Elsevier.
2.Anju Dahiya (2014). Bioenergy: Biomass to biofuels. Academic Press.
3.Maulin Shah, Susana R. C., Celia B. V. C., Jayanta B (2021). An Integration of phycoremediation
processes in wastewater treatment. 1st Edition. Elsevier.
4.Dieter D., Angelika S (2010). Biogas from Waste and Renewable Resources: An Introduction, 2 nd
edition. Wiley.
5.Neeraj K. Aggarwal, Naveen Kumar, Mahak Mittal (2022). Bioethanol Production: Past and Present
(Green Chemistry and Sustainable Technology). Springer.
 Suggested Reading
1.Manish Srivastava, Neha Srivastava, Rajeev Singh (2021). Bioenergy Research: Biomass Waste to
Energy. Springer.
2.Malgorzata K., Eleonore A., Kari-Anne L (2022). Biodegradable Waste Management in the Circular
Economy. Wiley.

Web Resources
1. https://onlinecourses.nptel.ac.in/noc19_bt16/preview
2. https://onlinecourses.nptel.ac.in/noc22_ch28/preview
3. https://onlinecourses.nptel.ac.in/noc22_ch05/preview

Course Articulation Matrix

Programme Outcomes
Programme Specific Outcomes
Cognitive Level
Course
Outcomes
PO1 PO2 PO3 PO4 PO5 PO6 PO7 PO8 PSO1 PSO2 PSO3 PSO4 PSO5
K1
CO 1 3 3 3 - - - 3 2 3 - 3 2 -
K2
CO 2 3 3 2 - - - 3 2 3 - 3 2 -
K3
CO 3 2 3 2 - - - 3 2 3 - 3 2 -
K4
CO 4 3 3 2 - - - 3 2 3 - 3 2 -
K5
CO 5 3 3 3 - - - 3 2 3 - 3 2 3

Wt. Avg. 3 3 2.4 - - - 3 2 3 - 3 2 3

Wt. Avg. 2.7 2.8

Overall Mapping of the Course With PO 2.7

With PSO 2.8
 Semester VI – Core Theory
Pharmaceutical Microbiology
Course Code
Credits 5
Hours / Cycle 6
Category Part Core Theory
Semester VI
Year of From the academic year 2023-24
Implementation
1.To understand and appreciate the importance of pharmaceutical
microbiology
2. To learn the commercial production of pharmaceutical products using
microorganisms
3.To acquire knowledge about Quality Assurance and Validation protocols
Course Objectives
in pharmaceutical industries
4.To perform microbiological analysis of pharmaceutical products
5.To know about microbial contamination, spoilage and standards of various
pharmaceutical products.

Bloom’s
Taxonomy
PSO
CO # Course Outcome(s) Levels
Addressed
(K1 to K5)

On completing the course successfully, the student will be able to

CO 1 Define the role of microorganisms in the PSO1, PSO2 K1

pharmaceutical sector. & PSO3
CO 2 Explain the various protocols followed in PSO1, PSO2, K2
pharmaceutical microbiology. PSO5

CO 3 Identify the standard microbiology protocols used in PSO1, PSO2, K3

pharmaceutical industries. PSO5

CO 4 Analyse the microbiological protocols of PSO1, PSO2 K4

pharmaceutical products according to statutory PSO3 &
standards. PSO5

CO 5 Assess the prospects of pharmaceutical PSO1, PSO2, K5

microbiology. PSO3, PSO4
PSO5

SYLLABUS
UNIT CONTENT HOURS COs BLOOM’S
90 TAXONOMY
LEVEL

I Pharmaceutical microbiology: Introduction and 18 1,2,3,4 K1,2,3,4,5

scope – Ecology of microorganisms in ,5
pharmaceutical industry – Formulation units and
active pharmaceutical ingredient manufacture units
(API).
II Pharmaceutical products from microorganisms – 18 1,2,3,4 K1,2,3,4,5
Commercial production of penicillin ,5
Bacterial and viral vaccines: types and commercial
manufacture - Microbial production and applications
of therapeutic/diagnostic enzymes: streptokinase and
streptodornase, β-lactamases, mammalian drug
metabolism
III Microbial contamination and standards of various 18 1,2,3,4 K1,2,3,4,5
pharmaceutical products - sterile preparations: ,5
injectables, ophthalmic suspensions, implants and
other parenteral preparations; their sterilization
processes – non-sterile pharmaceutical products -
Solid dosage forms; liquid orals; creams, lotions and
ointments; other parenteral formulations.
Preservation of pharmaceutical products.
IV Microbial limit test of pharmaceutical products - 18 1,2,3,4 K1,2,3,4,5
Sterility testing and pyrogen testing (LAL) of ,5
pharmaceutical products. Antibiotic bioassays:
diffusion assays, transferase assays - luciferase
assay. Applications of biosensors in pharmaceutical
industries – Concept of clean rooms, their
certification and monitoring of viable and nonviable
particles.
V Quality Assurance and Validation – validation 18 1,2,3,4 K1,2,3,4,5
protocols – Aspects of QC and QA in ,5
pharmaceutical manufacturing - GMP and GLP in
pharmaceutical industries –Regulatory agencies and
ICH: their role in drug standards, quality and safety
– certification systems – Drug discovery and clinical
trials - Drug licensing process in India. Central
Drugs Standard Control Organization. (CDSCO).
Prescribed Books/Textbooks
1.Denyer S.P., Hodges N., Gorman S.P. and Gilmore B. (2011). Hugo and Russell’s Pharmaceutical
Microbiology. 8th edition, Wiley-Blackwell.
2.Tim Sandle. (2015). Pharmaceutical Microbiology - Essentials for Quality Assurance and Quality
Control. Wood head publications
 References
1.Geoffrey Hanlon and Norman Hodges. (2013). Essential Microbiology for pharmacy and
pharmaceutical science. Wiley Blackwell.
2.S. P. Vyas & V. K. Dixit. (2003). Pharmaceutical Biotechnology. (2003) CBS Publishers &
Distributors, New Delhi.
3.Bhatia R and Ichpujani RL. (1995). Quality Assurance in Microbiology. CBS Publishers, New
Delhi.

Suggested Reading
1. Mehra P.S. (2011). A textbook of Pharmaceutical Microbiology. I.K. International Publishing
House Pvt. Ltd.
2.Richard Prince (2008) Microbiology in Pharmaceutical manufacturing. 2 nd Edition. Vol
I & II edited Published by PDA.
Web Resources
1. https://onlinelibrary.wiley.com/doi/abs/10.1002/9780470988329.ch15
2. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1927659/
3.https://www.sciencedirect.com/topics/pharmacology-toxicology-and-
pharmaceuticalscience/pharmaceutical-manufacturing

Course Articulation Matrix

Programme Outcomes
Programme Specific Outcomes
Cognitive Level
Course
Outcomes
PO1 PO2 PO3 PO4 PO5 PO6 PO7 PO8 PSO1 PSO2 PSO3 PSO4 PSO5
K1
CO 1 2 3 3 2 2 2 3 3 3 3 2 - -

K2
CO 2 2 3 - 2 3 2 3 3 3 3 - - 3

K3
CO 3 2 3 3 2 2 3 3 3 3 3 3 - 3

K4
CO 4 2 3 3 2 3 3 3 3 3 3 3 - 3

K5
CO 5 2 3 3 2 3 3 3 3 3 3 2 3 3

Wt. Avg. 2 3 3 2 2.6 2.6 3 3 3 3 2.5 3 3

Wt. Avg. 2.7 2.9

Overall Mapping of the Course With PO 2.7

With PSO 2.9
 Semester VI – Core Paper
Food and Dairy Microbiology

Course Code
Credits 5
Hours / Cycle 6
Category Part Core Theory
Semester VI
Year of From the academic year 2023-2024
Implementation
1.To learn about the importance of Microorganisms in food production and
preservation.
2.To study about microbial spoilage and contamination of different food
Course items.
Objectives 3.To gain knowledge on the usage of microorganisms in the manufacture of
dairy products.
4.To know about the microbiological quality of food products.
5.To differentiate between foodborne infections and intoxications.

Bloom’s Taxonomy
PSO Levels
CO # Course Outcome(s)
Addressed (K1 to K5)

On completing the course successfully, the student will be able to

CO 1 Recall the basic concepts of food PSO1, PSO2, K1

microbiology. PSO3, PSO4
CO 2 Explain in detail the role of microorganisms in PSO1, PSO2, K2
food and food processing industries. PSO3,
PSO 5
CO 3 Make use of the domain knowledge in food PSO1, PSO2, K3
and catering sectors PSO3, PSO4,
PSO5
CO 4 Analyse the microbiological protocols of food PSO 1, K4
products according to statutory standards. PSO 2, PSO5
CO 5 Assess the prospects of food microbiology. PSO1, K5
PSO2, PSO3,
PSO4, PSO 5

SYLLABUS
UNIT CONTENT HOURS COs BLOOM’S
90 TAXONOMY
LEVEL
 I Microorganisms important in food microbiology – 20 1,2,3,4, K1,2,3,4,5
General characteristics, classification and importance. 5
Food as a substrate for microorganisms. Principles of
food preservation–Asepsis/Removal of
microorganisms: Irradiation, anaerobic conditions,
temperature, drying, food preservatives

II Microbial growth and food spoilage –factors affecting 10 1,2,3,4, K1,2,3,4,5

microbial growth and survival in food – contamination 5
and spoilage of meat, fish, poultry, fruits and
vegetables - canned foods, intermediate moisture
foods.

III Sources of microorganisms in milk –contamination 20 1,2,3,4, K1,2,3,4,5

and spoilage of milk and milk products - 5
Microbiological analysis of milk – Lactic acid
bacteria: Probiotics, prebiotics and synbiotics -
Manufacture of yoghurt, kefir, koumiss, butter and
cheese. Microbes as food: mushroom, Spirulina, yeast.

IV Methods for microbiological examination of foods 20 1,2,3,4, K1,2,3,4,5

Good manufacturing practices (GMP), quality control 5
and HACCP - food control agencies, types of food
standards, certification systems and regulation.

V Biotechnology of dairy foods - GM foods – 20 1,2,3,4, K1,2,3,4,5

Production of sauerkraut, oriental fermented foods – 5
idli, tempeh, soy sauce, natto. Nutrition value of
fermented foods. Food-borne diseases – food-borne
infection and intoxication, risk factors associated with
food-borne illness, detection of food-borne pathogens.

Prescribed Books/Textbooks
1.Adams M.R., Moss M.O. and McClure P. (2016). Food microbiology. 4th edition. The Royal Society
of Chemistry, Cambridge, UK.
2.William C Frazier and Dennis C Westhoff (2017). Food Microbiology. 5th edition, McGraw Hill,
New York.
3.Robinson R.K. (2005). Dairy Microbiology Handbook 3rd edition. Wiley Interscience.
4.Bibek Ray, Arun Bhunia. (2013). Fundamental Food Microbiology. Fifth edition. CRC Press.
References
1.Motarjami Y and Lelieveld H (2014). Food Safety Management. Academic Press, Elsevier.
2.Peter J. Taormina, Margaret D.Hardin.(2021). Food safety and quality-based shelf life
of perishable foods. Springer.
3.Hutkins R.W (2018). Microbiology and Technology of Fermented Foods. 2nd edn. Wiley
Blackwell Publishers.
 Suggested Reading
1. Dairy Microbiology and Biochemistry. (2014). Edited by Ozer B. and Evrendilek
G.A., CRC Press, Boca Raton
2. James M. Jay, Martin J. Loessner, David A. Golden (2005). Modern Food Microbiology 7 th Edition.
Published by Food Science Text Series.
Web Resources
1.http://www.microbes.info
2.http://www.fsis.usda.gov/.
3.http://www.cdc.gov.
4.http://www.microbes.info/ resources/food microbiology
5.http://www.binewsonline.com/1/what is food microbiology.html.

Course Articulation Matrix

Programme Outcomes
Programme Specific Outcomes
Cognitive Level
Course
Outcomes
PO1 PO2 PO3 PO4 PO5 PO6 PO7 PO8 PSO1 PSO2 PSO3 PSO4 PSO5
K1
CO 1 2 3 3 2 3 3 3 2 3 3 3 3 -
K2
CO 2 2 3 3 2 3 3 3 - 3 3 3 - 3

K3
CO 3 2 3 3 2 3 3 3 3 3 3 3 3 3

K4
CO 4 2 3 - 2 3 3 3 - 3 3 - - 3

K5
CO 5 2 3 3 2 3 3 3 3 3 3 3 3 3

Wt. Avg. 2 2.6 3 2 3 3 3 3 3 3 3 3 3

Wt. Avg. 2.8 3

Overall Mapping of the Course With PO 2.8

With PSO 3
 Semester VI – Core Practical
Food, Dairy and Pharmaceutical Microbiology
Course Code
Credits 4
Hours / Cycle 6
Category Part Core Practical
Semester VI
Year of From the academic year 2023 -2024
Implementation
1.To develop the skill of performing the microbiological analytical protocols
related to food, dairy and pharmaceutical industries.
2.To observe and execute various microbiological analyses related to food and
dairy products.
3.To perform microbiological analysis of sterile and non-sterile
Course Objectives pharmaceutical formulations.
4.To administer isolation and enumeration protocols on microorganisms from
bakery products, fruits and vegetables.
5.To identify and relate the quality control / in-process results to the prescribed
standards by the governing bodies
Bloom’s
PSO Taxonomy Levels
CO # Course Outcome(s)
Addressed (K1 to K5)

On completing the course successfully, the student will be able to

CO 1 Recall and write the principles and procedures PSO 1, PSO2 K1

related to food, dairy and pharmaceutical PSO 3
microbiology.
CO 2 Explain the various equipment and materials used in PSO 1, PSO2 K2
food, dairy and pharmaceutical microbiology PSO3,
practical.
CO 3 Apply the microbiological techniques in food, dairy PSO 1, PSO2, K3
and pharmaceutical sectors. PSO3,
PSO 5
CO 4 Analyse the significance of each of the methods PSO1, PSO 2, K4
used. PSO 5

CO 5 Evaluate the methods and outcomes of all the PSO1, PSO 2, K5

experiments. PSO3, PSO 4,
PSO 5
 SYLLABUS
UNIT CONTENT HOURS COs BLOOM’S
90 TAXONOMY
LEVEL
1 Detection and estimation of bacteria in milk by 20 1,2,3, K1,2,3,4,5
standard plate count method. 4,5
Enumeration of bacteria in milk by Breeds
count method enumeration of microorganisms
in curd/yoghurt.
Preparation of yoghurt
2 Determination of milk quality 1,2,3,4 K1,2,3,4,5
Methylene blue reduction test ,5
Resazurin test 20
Litmus milk test

3 Isolation and enumeration of microorganisms: 20 1,2,3,4 K1,2,3,4,5

Bread and bakery products. ,5
Mayonnaise, spreads and sauces.
Fruits and vegetables
4 Sterility testing of pharmaceutical products 20 1,2,3,4 K1,2,3,4,5
Microbial limit test of raw materials/excipients. ,5
Sterility testing by Direct inoculation method
injectables, saline, ophthalmic preparations, surgical
cotton, gauze, surgical gloves, and soluble powders.

5 Determination of microbial load - non-sterile 10 1,2,3, K1,2,3,4,5

pharmaceuticals (syrups/tablets/creams/ophthalmic 4,5
preparations)
Determination of microbiological quality -
contact plate method (autoclaves/vessels/incubators
& packing materials.) Swab testing of irregular
surfaces/operators’ hands.
Evaluation of disinfection - Phenol coefficient
method - Rideal -Walker test, Chick martin test.

Prescribed Books/Textbooks
1. Mukerji K.G. (2013). Laboratory Manual of Food Microbiology. I. K. International Pvt. Ltd.
2. Mandal S. (2011). Laboratory Manual on Introductory Dairy Microbiology. ICAR-NDRI, Karnal.

References
1.Yousef A.E., Waite-Cusic J.G., Perry J.J. (2022). Analytical Food Microbiology: A Laboratory
Manual. (2nd Edition). Wiley.
2.Roberts D., Greenwood M. (2003). Practical Food Microbiology. 3rd Edition. Blackwell Publishing.
3.Pharmaceutical Microbiology Manual. (2020). Doc. No. ORA.007 / Food and Drug Administration
(FDA)
 Suggested Reading
1.Prasad G.S., Srisailam K. (2019). Pharmaceutical Microbiology: A Laboratory manual. Pharmamed
Press.
2.Shalini Sehgal (2020). A Laboratory manual of food analysis. Dreamtech press
Web Resources
1. https://nptel.ac.in/courses/126103017
2. https://www.classcentral.com/course/swayam-food-microbiology-14063

Course Articulation Matrix

Programme Outcomes
Programme Specific Outcomes
Cognitive Level
Course
Outcomes
PO1 PO2 PO3 PO4 PO5 PO6 PO7 PO8 PSO1 PSO2 PSO3 PSO4 PSO5
K1
CO 1 2 3 3 2 3 3 3 - 3 3 3 - -

K2
CO 2 2 3 3 2 3 2 3 2 3 3 3 - -

K3
CO 3 2 3 3 2 3 3 3 3 3 3 3 - 3

K4
CO 4 2 3 - 2 3 2 3 3 3 3 - - 3

K5
CO 5 2 3 3 2 3 2 3 3 3 3 3 3 3

Wt. Avg. 2 3 3 2 3 2.4 3 2.8 3 3 3 3 3

Wt. Avg. 2.7 3

Overall Mapping of the Course With PO 2.7

With PSO 3
 Semester VI – Core Theory
Parasitology and Mycology

Course Code

Credits 5
Hours / Cycle 6
Category Part Core Theory
Semester VI
Year of From the academic year 2023 -2024
Implementation
1.To study about the general characteristics of the medically important
fungi and parasites.
2.To acquire knowledge on the diagnosis, prevention and control measures of
fungal diseases.
Course Objectives 3.To list the medically important parasites and the diseases caused by them.
4.To learn about the outcome of host-parasite interactions.
5.To know the methods used for the examination of blood and faeces to
identify parasites.

Bloom’s
Taxonomy
PSO
CO # Course Outcome(s) Levels
Addressed
(K1 to K5)

On completing the course successfully, the student will be able to

CO 1 Define the various fungal and parasitic diseases of PSO1, PSO2, K1

humans and their diagnosis. PSO4 PSO5.

CO 2 Explain the clinical characteristics, specimen PSO1, PSO2, K2

collection methods, and diagnosis of medically PSO4, PSO5.
important parasites and fungi.
CO 3 Identify the characteristics, disease process, and PSO1, PSO2, K3
appropriate laboratory investigation methods of PSO3, PSO4
pathogenic parasites and fungi. PSO5.
CO 4 Compare the morphology, life cycle, pathogenesis, PSO1, PSO2, K4
laboratory diagnosis and treatment of various PSO3, PSO4
parasites and fungi. PSO5.
CO 5 Determine the medical importance of parasites and PSO1, PSO2, K5
fungi as a threat to human health. PSO3, PSO4
PSO5.
 SYLLABUS
UNIT CONTENT HOURS COs BLOOM’S
90 TAXONOMY
LEVEL
I Introduction to protozoa and Helminths. - Laboratory 20 1,2,3,4, K1,2,3,4,5
techniques in parasitology - Examination of faeces - 5
Direct and concentration methods. Morphology, life
cycle, Pathogenicity and laboratory diagnosis of
common intestinal protozoans – Entamoeba
histolytica and Entamoeba coli, Giardia lamblia,
Urogenital Protozoa – Trichomonas vaginalis, Blood
and tissue protozoans – Trypanosoma cruzi,
Plasmodium sp., Leishmania donovani, Toxoplasma
gondii.
II Morphology, life cycle, Pathogenicity and laboratory 19 1,2,3,4, K1,2,3,4,5
diagnosis of common Nematodes – Ascaris 5
lumbricoides, Wuchereria bancrofti, Trematodes –
Fasciola hepatica, Cestodes – Taenia saginata and
Taenia solium.
III Introduction and classification of medically important 14 1,2,3,4, K1,2,3,4,5
fungi. General characteristics of fungi. Collection of 5
clinical specimens for the diagnosis of fungal infection
- skin scrapings, nail clippings, skin biopsy and blood.
Cultivation of fungi from clinical samples.
IV Common methods employed in fungal diagnosis. 19 1,2,3,4, K1,2,3,4,5
Dermatophytes and agents of superficial mycoses. 5
Opportunistic mycotic infections - Aspergillus,
Mucor, Rhizopus and Candida.
V Yeasts and yeast like fungi of medical importance 18 1,2,3,4, K1,2,3,4,5
causing systemic mycoses - Coccidiomycosis, 5
Blastomycosis, Histoplasmosis and Cryptococcosis.
Antifungal therapy. Mycotoxins.
Prescribed books/Text Books
1. Jagadish Chander (2016). A Textbook of Medical Mycology, 4th edition, Jaypee (Publisher).
2. Chakraborty, P (2013). A TextBook of Microbiology. 3rd edition. Published by New Central Agency
(P) Ltd., Kolkatta.
3. Burton Bogitsh and Clint Carter (2018). Human Parasitology, 5th edition, Elsevier.
4. Chatterjee (2019). Medical Parasitology, 13th edition, CBS publisher.
5. Mehdi Razzaghi-Abyaneh (2020). Medical Mycology: Current Trends and Future Prospects, CRC
press.
References
1. Subhash Chandra Parija (2016). Textbook of Medical Parasitology – Protozoology and
Helminthology, 4th edition, published by All India Publishers and Distributors, Medical book
publisher, New Delhi.
2. Jeyaram Paniker. (2013). Textbook of Medical Parasitology. 7th edition, JAYPEE brothers, Medical
 Publishers (P) Ltd, New Delhi.
3. Christopher C. Kibbler, Richard Barton,Neil A. R. Gow, Susan Howell,Donna M. MacCallum, Rohini
J. Manuel (2017). Oxford Textbook of Medical Mycology. Oxford University Press.
Suggested Reading
1. Ananthanarayanan. R and Jayaram Panikaer, C.K. (2020). Textbook of Microbiology, 11th edition.
Orient Longman.
2. Kevin Kavanagh, (2018). Fungi Biology and Applications 3rd Edition. Wiley Blackwell publishers.
3. Alexopoulos C.J, Mims C.W, Blackwell, M. (2007). Introductory Mycology, 4 th edition. Wiley
publishers.
Web Resources
1. https://www.swayamprabha.gov.in/index.php/program/archive/9
2. https://mycology.imedpub.com/
3. https://academic.oup.com/cid/article/40/3/437/303735 (Oxford Academic - resources for Medical
Mycology)
4. https://www.cdc.gov/parasites/
5. http://mycology.cornell.edu/fteach.html (mycology resources)

Course Articulation Matrix

Programme Outcomes
Programme Specific Outcomes
Cognitive Level
Course
Outcomes
PO1 PO2 PO3 PO4 PO5 PO6 PO7 PO8 PSO1 PSO2 PSO3 PSO4 PSO5
K1
CO 1 2 3 - 2 2 2 3 3 3 3 - 3 3

K2
CO 2 2 3 - 2 2 2 3 3 3 3 - 2 2

K3
CO 3 2 3 3 2 3 2 3 3 3 3 3 3 2

K4
CO 4 2 3 3 2 3 2 3 3 3 3 3 3 3

K5
CO 5 2 3 3 2 3 2 3 3 3 3 3 2 3

Wt. Avg. 2 3 3 2 2.6 2 3 3 3 3 3 2.6 2.6

Wt. Avg. 2.6 2.8

Overall Mapping of the Course With PO 2.6

With PSO 2.8
 Semester VI - Core Practical
Parasitology and Mycology

Course Code

Credits 3
Hours / Cycle 4
Category Part Core Practical
Semester VI
Year of From the academic year 2023 -2024
Implementation
1.To Acquire practical skills in Parasitology and Mycology.
2.To understand the identification methods of parasites from stool samples.
3.To acquire knowledge on various staining techniques to identify the fungi.
Course Objectives 4.To learn the fungal cultivation methods.
5.To perform routine clinical laboratory procedures for parasitic and fungal
diseases.

Bloom’s
PSO Taxonomy Levels
CO # Course Outcome(s)
Addressed (K1 to K5)

On completing the course successfully, the student will be able to

CO 1 Define the identification methods of parasites and PSO1, PSO2, K1

fungi from clinical samples. PSO4, PSO5.

CO 2 Demonstrate the morphological features of parasites PSO1, PSO2, K2

and fungi, as well as the biochemical characteristics PSO4, PSO5.
of yeast.
CO 3 PSO1, PSO2, K3
Apply appropriate laboratory investigation methods PSO4, PSO5.
for the diagnosis of fungal and parasitic infections.

CO 4 Examine the specimens for the identification of PSO1, PSO2, K4

parasites and fungi. PSO4, PSO5.
CO 5 Evaluate the significance of various diagnostic PSO1, PSO2, K5
procedures to identify medically important parasites PSO3, PSO4
and fungi. PSO5.

SYLLABUS
 UNIT CONTENT HOURS COs BLOOM’S
60 TAXONOMY
LEVEL
I Examination of parasites in stool - 12 1,2,3,4, K1,2,3,4,5
Normal saline and Lugol's iodine mount. 5

II Examination of faeces by concentration methods - 12 1,2,3,4, K1,2,3,4,5

Floatation and Sedimentation methods. 5
Demonstration of common parasites.
III Demonstration of common fungal species - 12 1,2,3,4, K1,2,3,4,5
(Aspergillus, Mucor, Penicillium and Rhizopus) 5
Lactophenol cotton blue staining and Slide culture
technique.
IV Demonstration of Dermatophytes - 12 1,2,3,4, K1,2,3,4,5
Wet mount examination and Potassium hydroxide 5
preparation (KOH) of fungi.
V Identification of Yeasts - Germ tube method, 12 1,2,3,4, K1,2,3,4,5
Assimilation and fermentation tests for yeasts. 5

Prescribed books/Text Books

1.Monica Cheesbrough, (2005). District Laboratory Practice in Tropical Countries. Part 1, 2 nd edition,
Cambridge University Press.
2.Karyakarte, R.P. and Damle, A.S., (2005). Medical Parasitology. Revised edition. Published by Books
and Allied (P) Ltd., Kolkatta.
3.Chatterjee (2019). Medical Parasitology, 13th edition, CBS publisher.
4.Mackie & McCartney Practical Medical Microbiology (2008),14 th edition. Elsevier, New Delhi.
References
1. Chiodini P. L., Moody A. H., Manser D. W. (2001). Atlas of Medical Helminthology and Protozoology,
4th edition, Churchill Livingstone (publisher).
2.Subhash Chandra Parija (2016). Textbook of Medical Parasitology – Protozoology and Helminthology,
4th edition, published by All India Publishers and Distributors, Medical book publisher, New Delhi.
3. Cappuccimo, J. and Sherman, N. (2002). Microbiology: A Laboratory Manual, 6 th Edn. Pearson
Education Publication, New Delhi.
Suggested Reading
1. Baveja V. and Baveja C.P. (2021), Medical Parasitology, Arya Publishing Company.
2. Arora D. R. 2018. Medical Parasitology, 5th edition, CBS Publishers & Distributors Pvt. Ltd.
3. Alexopoulos C. J, Mims C.W, Blackwell M. (2007). Introductory Mycology, 4 th edition. Wiley
publishers.
Web Resources
1.https://www.digimat.in/nptel/courses/medical/microbiology/MB76.html
2.https://www.cdc.gov/parasites/
3. https://www.isham.org/mycology-resources/mycological-links (international society for Human and
Animal Mycology.
4. https://www.researchgate.net/publication/259670265_Introduction_To_Medical_Parasitology
 Course Articulation Matrix

Programme Outcomes
Programme Specific Outcomes
Cognitive Level
Course
Outcomes
PO1 PO2 PO3 PO4 PO5 PO6 PO7 PO8 PSO1 PSO2 PSO3 PSO4 PSO5
K1
CO 1 2 3 - 2 2 2 3 3 3 3 - 3 3

K2
CO 2 2 3 - 2 2 2 3 3 3 3 - 3 3

K3
CO 3 2 3 - 2 3 2 3 3 3 2 - 3 3

K4
CO 4 2 3 - 2 3 2 3 3 3 3 - 3 3

K5
CO 5 2 3 3 2 3 2 3 3 3 2 3 3 3

Wt. Avg. 2 3 3 2 2.6 2 3 3 3 2.6 3 3 3

Wt. Avg. 2.6 2.9

Overall Mapping of the Course With P0 2.6

With PSO 2.9

Weightage for Correlation

0 C 5% No correlation -
5% <C 40% Low / Slight 1
40% <C < 60% Moderate 2
60% C < 100% Substantial / High 3
 Syllabus Revision Details
Course Title Revised/Renamed/Replaced % of change
/Retained

Semester I

Introduction to Microbiology Revised 20

(Theory)

Introduction to Microbiology Revised 5

(Practical)

Biochemistry I(Theory) Revised 35

Biochemistry I (Practical) Revised 10

Invisible Lifeforms and its 25

impacts (Nomenclature
Revised & Renamed
changed Previous:
Microbiology in day today
life)

Semester II

Microbial Physiology and Revised & renamed 40

Metabolism (Theory)

Microbial Physiology and Revised & renamed 33

Metabolism (Practical)

Biochemistry II (Theory) Revised 65

Biochemistry II (Practical) Revised 50

Invisible Lifeforms and its

Impacts (Nomenclature
Revised & Renamed 25
changed Previous:
Microbiology in day today
life)

Semester III

Immunology (Theory) Revised 40

Immunology (Practical) Revised 10

Bioinstrumentation (Theory) Revised 40

Bioinstrumentation Revised 10
(Practical)

Health and Nutrition Replaced 100

Semester IV

Microbial Genetics & Revised 45

Molecular Biology (Theory)

Microbial Genetics & Revised 10

Molecular Biology
(Practical)

Biotechnology (Theory) Revised 100

Biotechnology (Practical) Revised 100

Health and Nutrition Replaced 100

Semester V

Bacteriology and Virology Revised 40

(Theory)

Bacteriology and Virology Revised 20

(Practical)

Industrial Revised 16
Microbiology(theory)

Environmental Microbiology Revised 50

(Theory)

Industrial and Environmental Revised 20

Microbiology (Practical)

Computer Training Revised 43

Bioenergy from waste Revised 100

resources

Semester VI

Parasitology & Mycology Revised 35

(Theory)
 Parasitology & Mycology Retained -
(Practical)

Food and Dairy Revised 10

Microbiology (Theory)

Pharmaceutical Microbiology Revised 8

(Theory)

Food, Dairy & Retained -

Pharmaceutical Microbiology
(Practical)

Curriculum Overview Table

Part Credits Hours / Cycle
I – Language 12 16
II - English 12 16
III – Core theory (mandatory) 50 57
III – Core Practical* 25 37
III – Allied theory 12 16
III – Allied Practical* 8 8
IV - GC 4 8
IV - GE 3 4
IV – ID 3 4
IV – EVS 2 4
IV – Computer Training 3 2
IV – Personality Development / Soft Skill 3 4
IV- Value Education 2 4
V – Extension Activity 1
Total 140 180