Name: Elleeze Gwyneth Esthella D.

Empiales ​ ​ ​ ​ ​ ​ ​ ​ ​ ​ Date: May 18, 2025

Subject & Section: MT 20 (LEC) - BB

CLASSIFICATION PURPOSE/USE
SAMPLE MEDIUM IMPORTANT COMPONENTS
ACCORDING TO USE

●​ Peptone
●​ When isolating and
●​ Lactose
differentiating
●​ Agar
1.​ MacConkey (MAC) Agar lactose-fermenting and
●​ Neutral red
non-fermenting Gram
●​ Crystal violet
Negative rods
●​ Taurocholate

●​ Casein digests ●​ When isolating and

●​ Lactose differentiating
2.​ Eosin-Methylene Blue
●​ Sucrose lactose-fermenting and
(EMB) Agar
●​ Eosin Y non-fermenting
●​ Methylene blue Gram-negative rods.

●​ Basal medium contains

glycerol, sodium pyruvate,
and thiamine
3.​ Ellinghausen-McCullough ●​ Supplements: bovine ●​ Selective for the growth of
Selective
-Johnson-Harris Medium albumin, Tween 80, vitamin Leptospira spp
Bi2, and salts of iron,
calcium, magnesium, zinc,
and copper.

●​ Peptone
●​ Tryptone
●​ Yeast extract​
●​ Lactose ●​ used for the isolation of
4.​ Brilliant Green Agar
●​ Sucrose Salmonella serovars except
(BGA)
●​ Sodium chloride​ S. enterica server Typhi.
●​ Phenol red​
●​ Brilliant green​
●​ Agar

●​ Peptone base agar ●​ when isolating Salmonella

5.​ Hektoen Enteric (HE) Agar
●​ Bile salts and Shigella spp. and
 ●​ Lactose differentiating these
●​ sucrose organisms from other
●​ salicin Gram-negative rods.
●​ ferric ammonium citrate
●​ pH indicators:
bromothymol blue and acid
fuchsin

●​ casein and soybean meal

●​ When recovering anaerobic
agar supplemented with
6.​ Kanamycin-Vancomycin Gram-negative rods
kanamycin vancomycin
Laked Blood (LKV) Agar especially Bacteroides and
vitamin K and lysed
Prevotella spp.
(laked) sheep blood.

●​ digests of casein and

animal tissue
●​ yeast extract
●​ sodium citrate
7.​ Thiosulfate Citrate Bile ●​ sodium cholate ●​ When recovering Vibrio
Salts Sucrose (TCBS) Agar ●​ oxgall (bile) spp.
●​ sucrose
●​ ferric citrate
●​ thymol blue and
bromothymol blue

●​ crystal violet
●​ Gentamicin ●​ Isolation of B. pseudomallei
8.​ Ashdown's agar (ASA) or
●​ Glycerol and is standard for testing
Ashdown's medium (ASH)
●​ neutral suspected melioidosis
●​ red. B. pseudomallei

●​ Bile salts ●​ Selective for the recovery of

9.​ Bile-Esculin (Enterococcus
●​ Sodium azide vancomycin-resistant
Selective) Agar (BEA)
●​ Esculin enterococci

●​ Casein
10.​ Phenylethyl Alcohol (PEA) ●​ Soybean agar ●​ Used to isolate
Blood Agar ●​ Phenylethyl alcohol and Gram-positive cocci
blood
 ●​ Basal medium like brain
heart infusion brucella
●​ When isolating and
Columbia Shaedler’s or
1.​ Blood Agar detecting hemolytic
tryptic soy
organisms.
●​ Blood of heep horse rabbit
and more

●​ digests of animal tissue and

gelatin
●​ beef and yeast extracts
●​ sodium pyruvate ●​ Isolation of Yersinia
2.​ Cefsulodin-lrgasan-Novob ●​ sodium deoxycholate enterocolitica, most
iocin (CIN) Agar ●​ neutral red commonly from stool
●​ crystal violet specimens.
●​ Cefsulodin
●​ Irgasan
●​ Novobiocin

●​ Isolation and identification

of ESBL producing
Differential Enterobacteriaceae,
●​ Enzyme Substrates (e.g. carbapenemase resistant
ONPG, CPRG, and MUG) Enterobacteriaceae, MRSA,
3.​ Chromogenic Media (CM) ●​ Chromogen VRE, P. aeruginosa,
●​ Chromophore Salmonella, Streptococcus
●​ May include antibiotics agalactiae, Vibrio, C. difficile,
Enterobacteriaceae, Yersinia
enterocolitica, Acinetobacter,
and others.

●​ Animal Tissue Digest

●​ Fructose
4.​ Cycloserine-Cefoxitin-Egg ●​ Used for the recovery of
●​ Cycloserine
Yolk-Fructose Agar (CCFA) Clostridium difficile.
●​ Cefoxitin
●​ Neutral Red

●​ heart infusion agar

5.​ Cystine Tellurite Blood ●​ potassium tellurite ●​ Used for the recovery of
Agar (CTBA) ●​ L-cystine Corynebacterium diphtheriae.
●​ rabbit blood
 ●​ Casein enzymic
hydrolysate
●​ Papaic digest of soybean
6.​ Egg Yolk Agar (EYA) / meal​ ●​ Used for the isolation and
Modified McClung-Toabe ●​ Yeast extract​ differentiation of
Agar ●​ Sodium chloride​ Clostridium spp.
●​ L-Cystine​
●​ Hemin
●​ Vitamin K1

●​ Oxgall (bile)
●​ Esculin
●​ Ferric ammonium citrate ●​ When recovering the
7.​ Bacteroides Bile-Esculin
●​ Hemin Bacteroides fragilis group
(BBE) Agar
●​ Vitamin and Bilophila wadsworthia.
●​ Gentamicin in a casein and
soybean agar base

●​ Lactose
8.​ Oxidative-Fermentative
●​ Casein digest
Polymyxin ●​ used for the isolation of
●​ Polymyxin B
B-Bacitracin-Lactose Burkholderia cepacia.
●​ Bacitracin
(OFPBL) Agar
●​ Bromothymol blue

●​ digests of casein and

animal tissue ●​ Isolation and identification
●​ beef extract of Salmonella enterica server
9.​ Bismuth Sulfite (BS) Agar
●​ Glucose Typhi and other enteric
●​ ferric sulfate rods.
●​ bismuth sulfite

●​ Peptone
●​ Sorbitol
10.​ Sorbitol-MacConkey Agar ●​ used for the isolation of E.
●​ Agar
(SMAC) coli 0157
●​ Neutral red
●​ Taurocholate
 ●​ Casein/Animal Tissue
Digest
●​ Growth of most bacteria
●​ Cornstarch
1.​ Chocolate Agar, Chocolate and is required for the
●​ Sodium chloride​
Blood Agar, or Chocolate recovery of many
●​ Dipotassium Phosphate
Agar Plate Haemophilus spp. and
●​ Monopotassium Phosphate
(CHOC/CBA/CAP) some pathogenic Neisseria
●​ Hemoglobin solution​
strains.
●​ Coenzyme enrichment
●​ Agar

●​ Animal digests
●​ Heart muscle infusion
●​ When recovering
2.​ Loeffler Medium ●​ Beef serum
Corynebacterium diphtheriae
●​ Egg
●​ Glucose

●​ Trypticase peptone
●​ Yeast extract
●​ Sodium chloride
●​ Sucrose
3.​ Burkholderia cepacia ●​ used for the isolation of B.
Enriched ●​ Lactose
Selective Agar (BCSA) cepacia
●​ Polymyxin B
●​ Gentamicin
●​ Vancomycin
●​ Crystal violet

●​ Beef or horse meat

●​ Peptones ●​ used for the recovery of a
4.​ Chopped-Meat Broth (CM) ●​ Yeast extract variety of bacteria,
​ ​ ●​ Sugars particularly anaerobes,
●​ Starch from clinical specimens.
●​ L-cysteine

●​ Beef and casein extracts

5.​ Haemophilus Test ●​ Starch
●​ used for susceptibility
Medium (HTM) Agar and ●​ Sodium Chloride
testing of Haemophilus spp
Broth ●​ Enrichments: Yeast extract,
hemin, and NAD

●​ Brucella agar ●​ used for the isolation of

6.​ Skirrow Brucella Medium
●​ Sheep blood Campylobacter spp.
 ●​ Trimethoprim
●​ Vancomycin
●​ Polymyxin B

●​ Chocolate agar
●​ Casein Peptone
●​ Meat Peptone​
●​ Corn Starch​
●​ Potassium Phosphate,
●​ Isolation of Neisseria
7.​ Martin-Lewis Agar dibasic and monobasic
gonorrhoeae.
●​ Sodium Chloride​
●​ Hemoglobin
●​ Dextrose
●​ Enrichment Bio-X
●​ Antibiotics V.C.A.T

●​ Potato Flour​
●​ L- Asparagine​
●​ used for the cultivation and
●​ Monopotassium Phosphate
isolation of Mycobacterium,
8.​ Lowenstein Jensen (LJ) ●​ Magnesium Citrate​
specifically Mycobacterium
Media ●​ Malachite Green​
tuberculosis from clinical
●​ Magnesium Sulfate​
specimens.
●​ Glycerol
●​ Egg Base​

●​ Beef heart
●​ Casein enzymic
hydrolysate
9.​ Sodium Azide Crystal ●​ used for selective
●​ Sodium chloride
Violet Blood Agar cultivation of Erysipelothrix
●​ Glucose
(SA-CVBA)​ rhusiopathiae.
●​ Sodium azide
●​ Crystal violet
●​ Agar

●​ pancreatic digest of casein ●​ A general-purpose,

●​ papain digest of soybean non-selective media
10.​ Tryptic Soy Agar (TSA) meal providing enough nutrients
●​ sodium chloride to allow for a wide variety
●​ Agar of microorganisms to grow.
 ●​ Todd-Hewitt broth
supplemented with yeast ●​ used for the recovery
1.​ LIM Broth
extract, colistin, and ●​ of group B streptococci.
nalidixic acid.

●​ Peptones
●​ Sodium phosphate ●​ Used for the isolation of
2.​ Selenite Broth
●​ Lactose Salmonella spp.
●​ Sodium selenite.

●​ peptone base
supplemented with:
●​ used for the recovery of
○​ yeast extract
Salmonella spp. (except
○​ Mannitol
Salmonella typhi and S.
3.​ Tetrathionate Broth, Hajna ○​ Glucose
enterica subsp. arizonae)
○​ sodium deoxycholate
from stool and urine
○​ sodium thiosulfate
specimens.
○​ calcium carbonate
○​ brilliant green

●​ casein digest, glucose, yeast ●​ Used for the recovery of

Enrichment
4.​ Thioglycolate Broth extract, cysteine, and aerobic and anaerobic
●​ sodium thioglycolate bacteria.

●​ Enrichment broth for Vibrio

●​ Sodium Chloride
5.​ Alkaline peptone water species from food, water,
●​ Peptone
and clinical samples.

●​ Yeast Enriched Peptone

●​ Peptic Digest of Animal
Tissue
●​ Pancreatic Digest of Casein ●​ Recommended for the
6.​ Peptic Digest Agar (PDA)
●​ Sodium Chloride selective isolation of
with Fildes and Nafcillin
●​ Corn Starch Haemophilus species
●​ Nafcillin
●​ Fildes Supplement
●​ Agar

●​ Proteose peptone ●​ Used for enumeration of

7.​ M-Enrichment Broth ●​ Yeast extract bacteria by membrane filter
●​ Dipotassium hydrogen technique and for
 phosphate preliminary enrichment of
●​ Sodium Chloride organisms on membrane
filter prior to using
selective media

●​ Basal medium: Beef extract,

Yeast extract, NaCl,
Peptone
●​ used to selectively grow
●​ Horse blood, lysed
Campylobacter species,
8.​ Campylobacter Enrichment ●​ Cefoperazone solution
particularly in food
Broth ●​ FBP solution
samples or environmental
●​ Trimethoprim lactate
samples
solution
●​ Vancomycin solution
●​ Cycloheximide solution

●​ Sodium Chloride
●​ Maltose
●​ Yeast extract
●​ For the cultivation and
9.​ Enrichment Broth for ●​ Bile salts no. 3.
enrichment of Aeromonas
Aeromonas hydrophila ●​ L-cysteine hydrochloride
hydrophila.
monohydrate
●​ Bromothymol Blue
●​ Novobiocin

●​ Sodium Chloride ●​ recommended for use with

●​ Pepsin a variety of culture media
●​ Sheep Blood for the cultivation of
10.​ Fildes Enrichment Agar ●​ Sodium Hydroxide. fastidious microorganisms
●​ Hydrochloric Acid that require blood
Concentration derivatives for optimal
●​ Demineralized Water growth.

●​ Agar ●​ For the cultivation and

●​ Ammonium Sulfate differentiation of
●​ Pancreatic digest of casein microorganisms based on
1.​ Aerobic Low Peptone ●​ Yeast extract their ability to utilize a
Simple/Basal/Primary medium
(ALP) Basal Medium ●​ magnesium sulfate variety of carbon sources
heptahydrate such as carbohydrates,
●​ Potassium chloride alcohols, and other
●​ Phenol Red acidogenic substrates.
 ●​ Substrate solution

●​ used for the culture of

●​ Peptone non-fastidious
●​ Yeast extract microorganisms, and for
2.​ Nutrient Agar ●​ Beef extract quality control and
●​ Sodium chloride checking purity prior to
●​ Agar biochemical or serological
testing.

●​ Beef extract ●​ used for the cultivation of a

●​ Yeast extract wide variety of organisms
3.​ Nutrient broth
●​ Peptone from clinical specimens and
●​ Sodium Chloride other materials

●​ Casein enzymic ●​ used for study of glucose

hydrolysate (dextrose) fermentation
4.​ Glucose broth
●​ Glucose where a pH indicator is not
●​ Sodium chloride desired

●​ L-Glutamic acid
●​ ammonium sulfate
●​ dipotassium hydrogen
●​ For the cultivation and
5.​ Basal Synthetic Medium phosphate
maintenance of
(BSM) ●​ monopotassium phosphate
Acinetobacter lwoffii.
●​ magnesium sulfate
heptahydrate
●​ Salt solution

●​ dipotassium hydrogen
phosphate
●​ For the cultivation and
●​ monopotassium phosphate
differentiation of
6.​ Pseudomonas Basal ●​ ammonium sulfate
Pseudomonas species based
Mineral Medium ●​ magnesium sulfate
on their ability to grow
heptahydrate
●​ on different carbon sources.
●​ Carbon source
●​ Trace elements solution

●​ Yeast extract
7.​ Peptone Yeast (PY) Basal ●​ For the identification of
●​ L-cysteine hydrochloride
Medium treponemes
monohydrate
 ●​ Peptone
●​ Pancreatic digest of casein
●​ Resazurin
●​ Salts solution

●​ Pancreatic digest of
peptone
●​ Yeast extract
●​ Maltose
●​ L-cysteine hydrochloride
8.​ TYM Basal Medium, ●​ For the cultivation of
●​ dipotassium hydrogen
Modified 1 Trichomonas species.
phosphate
●​ monopotassium phosphate
●​ ammonium sulfate
●​ Agar, noble
●​ L-Ascorbic acid

●​ INORGANIC SALTS:
○​ Calcium
Chloride•2H2O
○​ Magnesium
Chloride•6H2O
○​ Magnesium Sulfate
(anhydrous)
○​ Potassium Chloride
○​ Potassium Phosphate
Monobasic
●​ For the cultivation of
(anhydrous)
animal tissue culture cells
9.​ Eagle’s basal medium ○​ Sodium Chloride
used for the growth of
○​ And more
arenaviruses.
●​ AMINO ACIDS
○​ L-Arginine•HCl
○​ L-Cystine•2HCl
○​ L-Glutamine
○​ L-Histidine (free base)
○​ L-isoleucine
○​ L-leucine
○​ And more
●​ VITAMINS
○​ D-Biotin
 ○​ Choline Bitartrate
○​ Choline Chloride
○​ Folic Acid
○​ myo-Inositol
○​ And more
●​ OTHER
○​ Phenol Red (sodium)
○​ D-Glucose
●​ ADDITIONAL
○​ L-Glutamine
○​ Sodium Bicarbonate

●​ Pancreatic digest of casein

●​ Yeast extract
●​ Maltose
●​ L-Cysteine·HCl
●​ For the cultivation of
●​ dipotassium hydrogen
10.​ TYM Basal Medium, Trichomonas vaginalis
phosphate
Modified 2 and Hypotrichomonas
●​ monopotassium phosphate
species.
●​ Agar, noble
●​ L-Ascorbic acid
●​ Lamb serum, heat
inactivated

●​ Proteose peptone no. 3

●​ Soluble starch
●​ Agar ●​ For the cultivation and
1.​ Dunkelberg Maintenance
●​ Glucose maintenance of Gardnerella
Medium
●​ Disodium hydrogen vaginalis
phosphate
●​ Monosodium phosphate

●​ Proteose peptone no. 3

Maintenance
●​ Agar
●​ For the cultivation and
2.​ Maintenance of L Antigen ●​ Disodium hydrogen
maintenance of Neisseria
in Neisseria ●​ Sodium chloride
gonorrhoeae.
●​ Glucose
●​ Rabbit blood, defibrinated

3.​ Motility Test and ●​ Peptone ●​ For the cultivation,

Maintenance Medium ●​ Sodium chloride maintenance, and
 ●​ Agar observation of the motility
●​ Beef extract of Listeria monocytogenes.
●​ 2,3,5-Triphenyltetrazolium
chloride

●​ For the cultivation,

●​ Pancreatic digest of casein
4.​ Motility Test and maintenance, and
●​ Sodium chloride
Maintenance Medium, observation of motility in
●​ Agar
Gilardi nonfermenting
●​ Yeast extract
Gram–negative bacteria

5.​ Alcaligenes NAYE ●​ Nutrient Broth

●​ For the cultivation and
Medium (Alcaligenes ●​ Yeast extract
maintenance of Alcaligenes
Nutrient Agar Yeast Extract ●​ Agar
species.
Medium) ●​ Distilled water

●​ Agar, noble
●​ Glucose
●​ Acetamide solution ●​ For the cultivation and
6.​ Acetamide Medium ●​ Monopotassium phosphate maintenance of Trichoderma
●​ Calcium chloride dihydrate longibrachiatum.
solution
●​ CsC12 solution

●​ Disodium hydrogen
phosphateHPO4
●​ KH2PO4
●​ NH4Cl
●​ MgCl2·6H2O
●​ Resazurin ●​ For the cultivation and
7.​ Acetobacteroides
●​ CaCl2·2H2O maintenance of
glycinophilus Medium
●​ Trace elements solution Acetobacteroides
Composition
●​ Vitamin solution glycinophilus.
●​ Yeast extract solution
●​ Glycine solution
●​ NaHCO3 solution
●​ Disodium hydrogen
phosphateS·9H2O solution.

8.​ Achromobacter Choline ●​ NaCl ●​ For the cultivation and

Medium ●​ Agar maintenance of
 ●​ Choline chloride Achromobacter
●​ K2HPO4 cholinophagum and other
●​ MgSO4·7H2O bacteria that can utilize
●​ FeSO4·7H2O choline as a carbon source.

●​ Potato starch
●​ Monopotassium phosphate
●​ For the cultivation and
●​ Malachite Green
maintenance of
9.​ Acid Egg Medium ●​ MgSO4·7H2O
Mycobacterium
●​ Penicillin G
tuberculosis.
●​ Fresh egg mixture
●​ Glycerol

●​ Agar
●​ Dipotassium hydrogen
phosphate
●​ Heart muscle, solids from
infusion
●​ Peptic digest of animal
●​ For the maintenance or
tissue
cultivation of a variety
●​ Glucose
of anaerobic bacteria,
●​ Yeast extract
including Actinomyces
10.​ Actinomyces Agar ●​ NaCl
species, Eubacterium
●​ Pancreatic digest of casein
species, Fusobacterium
●​ Monopotassium phosphate
species, Propionibacterium
●​ Ammonium sulfate
species, and others.
●​ L-cysteine hydrochloride
monohydrate
●​ Soluble starch
●​ Magnesium sulfate
heptahydrate
●​ Calcium chloride dihydrate

●​ Charcoal ●​ For the transport of swab

●​ Agar specimens to prolong the
●​ Sodium Chloride survival of microorganisms,
1.​ Amies Modified Transport ●​ Disodium hydrogen especially Neisseria
Transport
Medium with Charcoal phosphate gonorrhoeae, between
●​ Sodium thioglycolate collection and culturing.
●​ KCl Addition of charcoal to this
●​ KH2PO4 medium neutralizes
 ●​ CaCl2·2H2O metabolic products that
●​ MgCl2·6H2O may be toxic to Neisseria
gonorrhoeae.: For the
transport of swab
specimens to prolong the
survival of microorganisms,
especially Neisseria
gonorrhoeae, between
collection and culturing.

●​ Agar
●​ NaCl
●​ Sodium thioglycolate
●​ For the maintenance—as a
●​ L-cysteine hydrochloride
2.​ Cary and Blair Transport holding medium—of
monohydrate
Medium clinical specimens during
●​ CaCl2·2H2O
collection or shipment
●​ Na2HPO4
●​ NaHSO3
●​ Resazurin solution

●​ Beef heart, solids from

infusion
●​ Agar ●​ For the cultivation and
●​ Soluble starch transport of Bordetella
3.​ Jones-Kendrick Pertussis
●​ Tryptose pertussis between clinical
Transport Medium
●​ NaCl isolation and laboratory
●​ Charcoal powder, activated cultivation
●​ Yeast extract
●​ Penicillin solution

●​ Sodium glycerophosphate
●​ Agar ●​ For the maintenance—as a
4.​ Listeria Transport ●​ Sodium thioglycolate transport medium—and
Enrichment Medium ●​ CaCl2 enrichment of Listeria
●​ Nalidixic acid species.
●​ Acridine solution

●​ (NH4)2SO4 ●​ For the transport and

●​ NaCl isolation of bacteria from
5.​ Reduced Transport Fluid
●​ K2HPO4 dental plaque, especially
●​ KH2PO4 Streptococcus mutans and
 ●​ Na2CO3 Streptococcus sanguis.
●​ EDTA (ethylenediamine
tetraacetic acid)
●​ Dithiothreitol

●​ Agar
●​ For the transport of
●​ Beef extract
Bordetella pertussis and
6.​ Regan-Lowe Semisolid ●​ Pancreatic digest of gelatin
Bordetella parapertussis
Transport Medium ●​ Soluble starch
isolated from clinical
●​ NaCl
specimens.
●​ Charcoal

●​ For the preservation of

●​ Sodium glycerophosphate
Neisseria species and other
●​ Sodium thioglycolate
7.​ Stuart Transport Medium fastidious organisms
●​ CaCl2·2H2O
during their transport from
●​ Methylene blue
clinic to laboratory.

●​ Pancreatic digest of casein

●​ Agar
●​ For the maintenance—as a
●​ NaCl
holding or transport
●​ Sodium thioglycolate
8.​ Campy THIO Medium medium—of Campylobacter
●​ K2HPO4
species isolated from
●​ Na2SO3
clinical specimens on swabs
●​ L-Cystine
●​ Antibiotic supplement

●​ Bovine serum albumin

●​ Veal infusion broth ●​ For the maintenance and
●​ Amphotericin B solution transport of specimens
9.​ Viral Transport Medium
●​ Gentamicin suspected of being virally
●​ Phenol Red infected.
●​ Vancomycin

●​ For the cultivation and

●​ NaCl
10.​ Alkaline Peptone Water transport of Vibrio cholerae
●​ Peptone​
and other Vibrio species.