MICROBIOLOGY QUESTIONS
CHOOSE THE BEST CORRECT ANSWER
1. Small, gray, moist and alpha hemolytic colonies on blood agar is
suggestive of
A. Staphylococcus aureus
B. Streptococcus agalactiae
C. Streptococcus pyogenes
D. Staphylococcus saprophyticus
E. Streptococcus pneumoniae
2. Large yellow colonies on TCBS Media from a stool sample of a
32 years old female with watery diarrhoea is suggestive of
A. Staphylococcus aureus
B. Clostridium difficile
C. Vibrio cholerae
D. Shigella dysenteriae
E. Salmonella typhimurium
3. Urine sample from a 72 years old man with permanent
indwelling urinary catheter grew a fruit smelling, non lactose
fermenting colonies with metallic tinge. The most likely
pathogen is
A. Proteus mirabilis
B. Yersinia enterocolitica
C. Pseudomonas aeruginosa
D. Morganella morganii
E. Seratia marcescens
4. Gram negative, kidney shape intracellular diplococci is
suggestive of
A. Vibrio cholerae
B. Neisseria gonorrhoea
C. Yersinia pestis
D. Brucella abortus
E. Francisella tularensis
5. The fluorochrome stain used in the demonstration of
mycobacterium tuberculosis in fluorescent microscope is
A. Auramine O
B. Rhodamine B
C. Nigrosin
D. Eosin
E. Metachrome
TRUE AND FALSE
1. Regarding sample processing in microbiology laboratory
A. Inter-laboratory EQA is among the quality management
system FALSE
B. Competence assessment is done to the laboratory manager
FALSE
C. 20% sulphuric acid is the decolourizer for Ziehl -Neelsen
stain TRUE
D. Alcohol is the best decolourizer in gram staining technique
FALSE
E. SD Bioline and Determine is the serial National guideline
for HIV -1 testing in Tanzania FALSE
2. Regarding sexually transmitted diseases
A. Aspirate from the inguinal swelling (bubo) can be used in
diagnosis of treponema pallidum FALSE
B. Urethral discharge is the best sample for diagnosis of
chlamydia trachomatis TRUE
C. Blood is the only sample that can be used to diagnose
klebsiella granulomatous FALSE
D. Urethral discharge can be used in diagnosis of
mycoplasma genitalium TRUE
E. Ulcer discharge can be used in diagnosis of Neisseria
gonorrhoea FALSE
MATCHING ITERM
PART ONE
S/N ANSWER COLUMN A COLUMN B
1 D 10% KOH A. Cerebral spinal fluid
2 A Cryptococcus B. Locomotion
neoformans
3 F Simple stain C. Phenol
4 H Bacillus D. Fungal elements
stearothermophillus
5 C Ziehl Neelsen stain E. Hot air oven
F. Methylene blue
G. Gram positive cocci
H. Autoclave control
PART TWO
S/N ANSWER COLUMN A COLUMN B
1 C Enterobacteriaceae A. Methicillin resistant
staphylococcus aureus
(MRSA)
2 A Nosocomial B. Inhibits protein
infection synthesis
3 D Bacillus cereus C. Extended spectrum
beta-lactamases
(ESBL)
4 E Haemophilus D. Food poisoning
influenzae
5 B aminoglycoside E. Microaerophilic
F. Vibrio cholerae
G. Mycobacteria leprae
SHORT ANSWER
1. Mention five (5) pathogenic gram positive bacteria which can
cause food poison
I. Bacillus cereus
II. Clostridium perfringens
III. Clostridium botulinum
IV. Staphylococcus aureus
V. Streptococcus pneumoniae
VI. Listeria monocytogenes
2. Outline five (5) ingredients of Agar
I. Meat extract
II. Yeast extract
III. Carbohydrate
IV. Peptone water
V. Agar
VI. Distilled water
3. List 5 main types of culture media
I. Basic/General purpose media
II. Selective media
III. Differential/indicator media
IV. Enriched media
V. Enrichment media
VI. Indication media
VII. Sesitivity media
VIII. Transport media
4. List five uses of standard microorganisms
I. Used as a control materials
II. Used in research purposes
III. Used in study purpose
IV. Used in production of vaccines
5. Mention at least 5 materials used for preparation of culture
media
I. Autoclave
II. Weigh balance
III. Autoclave tape
IV. Hot plate
V. Measuring cylinders
VI. Petri dishes
VII. Beakers
VIII. Spatula
IX. Agar powder
6. Mention 5 reason why Muller Hilton recommended as
sensitivity media
I. Is isotonic
II. Has stable pH
III. It is well defined
IV. Grows the organism rapidly
V. Does not lead to antagonism
7. Mention factors that facilitate growth of candida albicans
I. Temperature
II. Moisture
III. Ph
IV. Nutrients
V. Air
VI. light
8. Mention 5 biochemical test for mycobacteria tuberculosis
I. Susceptibility to p-nitrobenzoic acid (PNB)
II. Niacin test
III. Catalase test
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