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MCB 201 M, Q, A.by Joel

The document provides an overview of microbiology, detailing various branches such as bacteriology, mycology, and virology, along with the structure and functions of bacteria. It discusses the nutritional categories of microorganisms, their growth requirements, and the influence of environmental factors on microbial growth. Additionally, it covers the classification of bacteria based on shape, arrangement, and modes of nutrition, including autotrophic and heterotrophic types.
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0% found this document useful (0 votes)
17 views54 pages

MCB 201 M, Q, A.by Joel

The document provides an overview of microbiology, detailing various branches such as bacteriology, mycology, and virology, along with the structure and functions of bacteria. It discusses the nutritional categories of microorganisms, their growth requirements, and the influence of environmental factors on microbial growth. Additionally, it covers the classification of bacteria based on shape, arrangement, and modes of nutrition, including autotrophic and heterotrophic types.
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
Available Formats
Download as PDF or read online on Scribd
————— > $07 ee ee Branches Microbiology L Bacteria wy, | Different form of bacteria, / Microbial nutrition v ie ee Cultivation and growth of microorganisms Growth requirements of microorganisms Reproduction of bacteria _ ¥ Types of media for microbial growth Nutritional Categories of microorganisms / 4 Modes of culturing microbes: Pure culture techniques; 4 batch culture; continuous culture Phases of growth in | batch culture j Nvironmental factors influencing microbial growth w Antimicrobial agents: definitions of terms (i) Antibiotics’ (ii) d] Static and cidal agent (iii) sterilization (iv) disinfection (v) Sanitization (vi) antiseptic | Structure and functions of cell wall, outer member, } Periplasm, cell member, pilli, flagella etc. v | Shapes and arrangements of bacteria. .~ * Structure of enzyme be Microscopy | ; : | Some Exam key point Sp.sq Past Question : ; ——a | Se oe os Branches Microbiology ified into pure an The branches of microbiology can be class sified based o applied sciences. Microbiology can be mycology, protozoolog: taxonomy, in the cases of bacteriology, ay atween the specifi and phycology. There is considerable ove! “with other discipline: branches of microbiology with each other an end beyond th and certain aspects of these branches can ext traditional scope of microbiology. i . Bacteriology: The study of bacteria. Mycology: The study of fungi. Protozoology: The study of protozoa. Phycology/algology: The study of algae. Parasitology: The study of parasites. Immunology: The study of the immune system. Virology: The study of viruses. Nematology: The study of nematodes. ; Microbial cytology: The study of microscopic and submicroscopic details of microorganisms. aan ! Microbial physiology: The study of how the microbial cell functions biochemically. Includes the study of microbial growth, microbial metabolism and microbial cell structure. {- Microbial ecology: The relationship between microorganisms and their environment. yw Microbial genetics: The study of how genes are* organized —_and regulated in microbes in relation to their cellular functions. Closely related to the field of molecular biology. Cellular microbiology: A disci and cell biology. a: Evolutionary microbiology: The study of the evolution of microbes. This field can be subdivided into: Microbial taxonomy: The naming and classifi microorganisms. Microbial systematic: The study of the diversity and 7 genetic relationship of microorganisms. 6 Generation microbiology: The study of those microorganisms that have the same characters ac iéir parents. By ju Systems microbiology: bio'ogy and microbiology. “e Mc lecular microbiology: The study of the molecular 2 pline bridging microbiology ication of A discipline bridging systems Principles of th i ] microorga a Physiological processes ty Biolog t f which are re nt: The study of those microorganisms q5 {Predictive mare Used in weapon industries. | between co; 'Crobiology: The quantification of relations - Pathogenic aroling aoe in foods and responses of Spoila i i i mathematical modellicg microorganisms using lel me of the Major isdn Microb | Medical microbiology Microbiology | Environmental Microbiology | i1 {De Immunology Industrial Microbiology E f Agricultural Microbiology BACTERIA | cteria are omnipresent, occurring in all environmenis. They } cur in water, in air, in soil, and in or on the bodies of living nisms, There are bacteria which occur in hot springs as well as reme cold temperature. Bacteria, despite their simplicity, din a well-developed cell structure which is responsible for 1 nite of their unique biological structures. Many structural Sd); itt re unique to bacteria and are not found among archaea or > Because of the simplicity of bacteria relative to larger st “5 and the ease with which they can, be manipulated ¥ ‘ e@rimentally, the cell structure of bacteria has been well studied, paling many biochemical principles: that sequently applied to other organisms. of \ Ss j DIFFERENT FORMS OF BACTERIA \ veos ave the common forms of bacteria. i (sin SOCCUS) to | Ao arieal has, They can be further distinguished : have been. «© | eens * } | | j coccus (oan sina icoccus (occur in pails, ur in groups of four) Bee ees (occur in the form of a flament) and hylococcus (occur in the form of sheets), 3 tl A steal “A ] | Examples: Sarcina ventriculi, Sarcna ureae, ete | eae cs 0 |! f boy & e 5. Staphylococci - The cocci are arranged in grape-like clusters f formed by irregular cell divisions in three plains. | f Examples: Staphylococcus aureus ‘ se a] Sant ‘ pc LhuUSs Soe a Bias They are straight, cylindrical, rog shaped bacteria, They may be further distinguished into Monobacillus (single) Diplobacillus (in pairs) Streptobacillus (in filamentous form) and i Palisade Bacillus (in the form of a Stack) | Arrangement of Bacilli he cylindrical or rod-shaped bacteria are called bacillus’ (plural: bacilli). 1. Diplobacilli Most bacilli aPPeal as single rods, Diplobacill appear in pairs after division Example of Single Rod: Bacillus Cereus! Rees. 5 a burnetii, Moraxella_bovis, Examples of Diplobacilli: Coxiella Klebsiella thinoscleromatis, e Puss] ayn ged in chains, as the cells 2. Streptobacilli - The bacilli are arran bacillus monoailiformis divide in-one plane. Examples: Strepto! ae gf ‘Sinemretroaitt ~ ro. \ 3. Coccobacilli - These are so sh ; a ort and stumpy that $ appear ovoid they look like coccus and a bacillus. Examples: Haemophilus i inaii CHa aaeeea ite influenzae, Gardnerella vaginaiis, and cuenaaete : ere L A Spirillum Hees ae are ae Goiled like a corkscrew the cell divisions, resulting ne ue Forte of aston Oe picket fence and angul Example: Corynebacterium dipht that look like Chinese letters Vibrio these are elongated, C shaped or comma shaped bacteria. Flagellation.Most of the bacteria contain one to many locomotor structures called flagella. Cocci do not have flagella. Based ‘on the number and mode of attachment of the flagella, bacteria can be distinguished into the following types: a.) Atrichous : Bacteria without flagella. b.) Trichous: They bear one or more flagella. c.) Cephalotrichous : Bacteria with many flagella attached at oneend d.) | Amphitrichous : Bacteria with a single flagellum at each end e.) Monotrichous : Bacteria with a single flagellum, atone end Example: Vibrio cholera and Pseudornonas sp. f.) Lophotrichous : Bacteria with a group of flagella attached at each end Example: Spirillum g.) Peritrichous : Bacteria with flagella all over the body Example: Salmonella Typhi and E coli. (These three flagellations are called polar arrangements) = Pili and(singular fimbriae- These are short thin, straight, Nair like projections found on . the surface of some bacteria. Both are proteins, they are smaller than the flagella Fimbriae (sometimes called "attachment pili") are fine filaments of protein, usually 2-10 nanometers in diameter and up to several micrometers in length. They are distributed over the surface of the cell, and resemble fine hairs when seen under the electron microscope. | Fimbriae ate believed to be involved in attachment to solid surfaces OF to other cells, and are essential for the virulence of some bacterial pathogens. Pili (sing. pilus) are cellular appendages, slightly larger than fimbriae, that can transfer genetic materia! between bacterial cells in a process called conjugation where they are called conjugation pili or "sex pili” (see bacterial genetics, below) They can also generate movement where they are called type IV pili (see movement, below). Their differences Y Pilli are found only in gram ni are found in both, gram nega! Fimbriae are shorter in length compared to pill. Pilli are more numerous than fimbriae. Fimbriae are less rigid than pill. Pilli are responsible fort bacterial conjugation but fimbriae are responsible for cell to surface attachment, Formation of pilli is governed by plasmid genes but fimbriae is not. egative bacteria but fimbriae tive and positive bacteria. and smaller in diameter as Mode of Nutrition and Nutritional Categories of Microorganisms They exhibits different modes of nutrition level such as- Autotrophic bacteria:. These bacteria are able to synthesize their own food. For e.g.: Phototropic bacteria and chemosynthetic bacteria ~ Heterotrophic bacteria: These bacteria are unable to size their own food, hence they depends on other organic synthe: materials. For e.9.: saprophytic bacteria-these bacteria feeds on dead and decaying matte symbiotic bacteria: These bacteria have a mutual benefit from other organisms. For e.g.: nitrogen fixing bacteria 8 \ -S-, | | | (or) rhizobium. . I Parasitic bacteria: These bacteria are present in plants, animals and human beings. These bacteria feeds on host cells and causes harm to the host. = Nutritional types in bacterial metabolism Nutritional | Source of Source of carbon | Source of carbon type energy Phototrophs | Sunlight | Organic compounds | cyanobacteria, Greensu | (photoheterotrophs) ffur | or carbon fixation | hacteria, Chloroflexi, or (photoautotrophs) | purple bacteria ee ees ee Lithotrophs | Inorganic | Organic compounds Thermodesulfobacteria compounds | (lithoheterotrophs) | Hydrogenophilaceae, or or carbon fixation —_| Nitrospirae (lithoautotrophs) Organotrophs | Organic | Organic compou nds | Bacillus, Clostridium or compounds | (chemoheterotrophs) | Enterobacteriaceae or carbon fixation (chemoautotrophs) ZHOTOAUTOTROPHIC BACTRIA jacteria form the largest number of livin i n ke ig Organisms on the planet and are habitable in all types of environments. Being as diverse as they are, theory mode of nutrition is Alco varied The photoautotrophic bacteria, belong to that type of bacteria that are Some ° eynshesizing their own food with the help of sunlight, that @ process of photosynthesis hs or photoautotrophic bacteria, u Shatere hence aulcrophe o Types of bacteria based on nutritional modes ‘asing on the modes of Nutrition . bacteria can be classified in to 9 | 4 ai lttues le the photoautotrophs, chemcautotraphs, Lithotrops ete, the folowing classification ean be made: Photeautotrophie bacteria synthesize theit own organic corspounds using sunlight as the enefgy, tource and carbon ‘onde as the carbon source Chemaautotrophic bacteria utilize carbon dioxide and produce ganic compounds using theenergy nimple inorganic stances, Photoheterotrophie bacteria use sunightas.an energy source but ‘heir carbon mustcome from arganic compounds, no! Ce, Chemoheterotrophie bacteria include parasitic types that draw nutrition trom living hosts, and saprophytic types thal obtain futition fom products, westes, or remains ofother organisms ‘Types of Bacteriochtorophyl ‘The photoautotrophie bacteria has bacteriochlotophyll wich it Uses for photosynthesis end can fx carbon acteriochlorophyll are {elated 1 chlorophyll andre the fundamental components neaded for photosynthesis in algae, cyanobacteria and plants. Those that contain bacteriochlorophyll do nol produce oxygen, and different wavelengths are usedby the pigment light energy HS 5, chormons 10 | | Examples of photoautotrophic bacteria Pleurocapsales spp. Nostocalies spp Rhodobacter "spp. ele, Predactous fungi ZThere are many animal tapping fungl which have develope ingenious mechanisms for capturing small animals. such’ ac~ gelvorms, rotfers oF protozoa which they use for food. Sever) PPecies of fungi in the genera Arthrobotrys. Dactyelis ary Gactylaria ullizes a rapidly constricting ring around a nematoas apa hold aptve while the hyphae sink haustoria into the bagy flihe victim, Some predacious fungi seerete a sticky substance the ‘surface of their hyphae to which a passing small anol adheres. Haustorum like hyphae then grows into the body of te animal and absorbs food, The animals ultimately die, Call Structure of Domain Bacteria Intracellular Structure A bacterium contains a wel-developed call structure wich ie * Gebenslble for many other unique biological properties. It can be assied into intaceluarsructureandextra-callarstructins, Intracellular structure Te Bacterial eli surrounded by cell membrane, which hols the cyleone, Ptolcins, and otter esseniial compounds ‘of tne gemlasm As bacteria are prokaryotes they do nat land ts hae stepgane- bond erganeles, so they contain few largeintraseiase structures, The organelles such 2s Golgi apparatus ana endooiasmic reliculum are present in bacteria whereas the organelles. such as ndria, and chloroplast are absent. nusieus, mit Cell Structure of Domain Bacteria : Extra Cellular Structure . ‘Bacterial cell is made up of peptidoglycan. Bacterial cell is completely different from the cell walls of plants and fungi, made up of chitin and cellulase. This cell wall is very essential to survival of ‘many bacteria, There are two types of cel wallin bacteria they ar Gram-positive and Gram-negative. Gram-positive bacteria — have a thick wall containing ‘many layers of peptidoglycan and teichoicacids. ‘Gram-negative bacteria - contains a thin wall consisting ofa few layers of peptidoglycan which is sufrounded by a second ~-— lipid membrane. i f Extra-cellular structure =, epee N Be <= BS ae ee GS : ki SSS Extra-celular is dependent on bacterial secretion system. The Ebove cagram shows the growth ofbacilus athress in cerebrospinal Mu blue green algae, E.co, eto. Parts of Prokaryol¢ Cell and their Functions that isnot found in : bacteria Example: Bacteri | capsule: itis composed of a thik polysaccharide. tis akind of {slime layer, which covers the outside ofthe cll wall. Iti used to stick cls fogether and works asa food reserve and it also protects the cel from dryness and from chemical. 12 SS |, mewurarie. wesosome plays @ Vist role in celular respirations, | eplication of DNA, cell division, separation of chromosomes + 7 during cell division and also performs the role of Golgi bouts: | and mitochondria, gi bodies ‘STRUCTURE, SHAPES AND ARRANGEMENTS OF | 4 BACTERIA | Bacteria are prokaryotic and unicellular microorganisms. Their cell structure is simpler than thase of other organisms called | eucaryotes. Basioally they lack true nucleus and membrane bound organelles. Structures often observed in bacterial call ae + summarized and ilustrated in Fig 1. Note that no single bacterium possesses af ofthe suture aa ings, Same are found only in certain cells in certain condi eri ae aah tas in conditions or in certain Like all ces, 2 typical bacterial cell has three sections ‘The Cell envelope; -The Cyloplasm; The extemal structures ~~ STRUCTURE OF A EACTERIRE CELE (A IHS CELL ENVELOPE —Al he layers surounding the _ tate membranes ner most ayer ofthe cell envelope utich ra, cll a= chemically complex, covers the plasma 1 cp CU2UEN Capsule or slime layer outside the cal val in "Sputum Suns ade oe a atone, “A THE EXTERNAL STRUCTURES — Extend from the cell - 13 surfece into the envionment, “Help the bacferium to respond to and maniter the external envkonmente - “Consists ofthe flagella and the pili or fimbrie, membrane- Also called the'coltmembrane, tt has the (biayes) of vane structure composed of 2 layer }—phasphoipd witin wich protein fst A pars, phespne Eromorene only allows water, gases and a few small molecules 10 ‘Tove feely tough fe while aging back the other molecules. iS frevelore 2 semipermeable membrane, Most membrane Essocated phospholinids are amphipathic. have polar heads {eystophyic) and nonpolar (hydrophobic) tls. The hydrophobic unbranched faty acdchains. -polaror hydrophilic heads" Ike to stay in an aquends environment ‘sing the outside worldand the insid= ofthe cal Hton-polar or bydrophobie tels" want to be away from water, M renvironment The tals are in the canter ofthe membrane. In ther words, ter hycrophobie region a fhe ryarephile portion project from the membrane surta _ The lipid composition of bacteria PM varies with erviranmental Jemeretyre n such a way thatthe membrane remain Mut uring Sronth, For erample bacteria growing at owe higher temperate Reve more unsaturated their membrane FESpnotpids. At higher temperature, their phospholpics 0% eresried tally acids. Bacteal membrane usualy cfr tom [eiSiyeti membranes in lacking sterol (sere containing fds) Faeeet many bacterial membrane contain sterol Hike molecules re ule inthe lipid whe dhopaniogs oe of bacterial membrane proteins have identi Three ‘yp! Indthey nave different functio integral prot substancy, ac ‘rechanisms known a Sethe cytoplasmic membrane. in 3 Miniporteymportand antiport. dutersurface proteins veual shh prilasmi proteinsin the transo esl. Fees urtace proteine: cooperate wth other proteins in ener inne eationsandeleo ober mpotantealar functions ort oflarge molecules into A aoarly af bacteriat coll walls contain peptidoglycan (murein) which has pores. peptidoglycan ipa signature molecus fr bacteria, No other organisms have been Ganstosynmeszet is rosea ofrany dete! subunts Each subnitcon _Szatgucosamine (NAG) and N/ acelymuramic perrnn sel deren arino acs, Tree of hesa amino acs 21 not rd in icine Dogktame acid, ‘D-slanine - —ianinopinaicecid. © Sr Ortanine, and meso: ee ann ~ rae il enn cise rane (7- to 8-nm-thick). aemee einsfirmly embedded In the membrane tianspoq— lyin Gram-negative bacteriainteract a: yeriplasmic space. The substance that occupies me espe cuca na aie ea eC hess i ds Be cal wats mansrne rotoplest are csin when Gram postive batera ar exposed ayscame rp Feeplte es afthe clive, tolysozyme or penicilin resulting Itistheretore surrounded by only jigsmamembrane. GramPositive envelope “The table below shows the differences of cel wall structure in gram posiveand gram-negative bacteria, re [2 meena erates oat = eae by a Gram stain - The different stating characleistic is besed on diferences In the structure of their cell walls. Gram postive cell wall consist ofmany layers ofpeptdoglycan (cross-lnkedby leichoie acid and lipoteichoic acd). Gram negative cell walls have ‘one or very few layers of peptidoglycan, surrounded by lpid-based outer membran Glycocaly, Capsule and Stime layer. Glycocalyx ‘refers. to 9 layer consisting of @ network of polysaccharides extending from the surface ofthe gell. The term | ‘ean encompass both capsules add slime layers because they usually are composed of polysaccharides. Itcanbe thick and stabla lke capsule or losely attached to cell walike sim layer. 3 Functions:- 1. Aids in attachment fo solid surfaces, including tissue surfaces. 2, Protects pathogenic bacteria fom the attack of the host's immune system. Slime layer is a zone of disse, unorganized material that is removedeaslly. wolved in attachment of bacteria to other cells or "Functions inanimate surfaces to form bofins, -Canalso be usedasa food reserve forthe cell 8 THE CYTOPLASM Bacterial cytoskeletons- similar in stucture and function with those of eukaryotes: Funetions: Participate incell division 7 pe Localize proteins to cartan sites inthe cal, Determine cell shape Inclusions Inclusions are considered to be nanlving components ofthe cell that do not pocsees matabole avy ang are nt bounded by ‘membranes. The most common inclusions are glycogen, Ipid Crystals, and pigments, Volutin granules are eyloplasiie inclusions ef complexed inorganic polyphosphate. ‘These granulze are called metachromatic granules due to their displaying the metachromatic effect they appear red ot blue ‘when stained wih the blue dyes methylene blue or toluidine bie. Functions: 1. Many inclusions are used for storage (eg. of carbon ‘compounds, inorganic substances, and energy. Used fo reduce osmotic pressure by tying UP in particulate for. 3. Many serve 2s micro compartments sultable for functions oth than storsgs. Eg. the carboxysome, a micto-compartment for carbondionde fixation, Gas vacuole provides buoyancy to many of which are photosyntnetc. Bacterial endospores —An endospore, a heat-resistant and rnon-growing svusture (dormant), formed within some bacterial Calls. An endospore can retain is viablty over long periods of time under adveres environmental conditions. When the environment bacome more favourable, the endospore then germinates to a vegetative cell They are produced by certain embers of bacteria belonging to the genera Bacilus, - Ciestridium and Sporosarcina. Features — endospores are extigordinariy resistant to ‘environmental stresses such as heat, Uittraviolont radiation gamma radiation, chemical disinfectants ‘and desiccation. Several species of endospore-forming ba re dangerous pathogens. E.g. Clostridium botulinum causes, 18 botulism, a food-borne disease, Clostridium totani causes tetanus and Bacillus anthracis fs causes anthrax. Coll Wall: Cellwals ofbacteriaare made up ofglycoprotein mute, “Themain arco fal walls Wels Inproving suppat; > ‘michoicalovonginand dy toca, {tproteets cell from bursting in a hypotonic medium, Plasma Membrane: It is also known 2s cytoplasmic.membrane (of) col ‘membrane, It is composed of phospholipids, proteins and ~~ carbohydrates, forming aMuid-mnosaie, |thelps in transportation of substances including removal of ‘wastes from the body. oo Ithelpsin providing a mechanical bari tothe cell. Plasma membrane acts 2s a semi permeable membrane whieh allows only selected material to move inside sed oulsideot the cell cytoplasm: weno Helps in celuar growth metabotsm andepication Gytopiasm is the store houses of al tye chetcals and components hatare used sustain the fe ofabactorumn Ribosome: oe Any granule made up of RNA and proteins, They ae hese of roi syinons rs ea rely Neatingsbustures that help in ancering. the genetic code. " ad Plasmi Plasmids are small citcle of DNA, red Bacterial cells have many plasmids. 19 Plasmids are used to exchange DNA between the 4 bacterial cel. t This is @ rigid rotating tal, i ~ ~—=-Ithelas the cell to move in clockwise and anticlockwise, forward and also helps the cell to spin | Chemotaxis: movement of bacteria toward or away trom chemical stimuli : . Magnetotaxis: movement along the Earth’s magnetic 3 i 5 5B 8 é 5 Phototexis: response to diferences in ight density. Bacteria ewim to areas of paricular light inst, Short protein apy ‘Smaller than fagela Fixes bacteria to surfaces, . +___Itelso helas in reproduction during conjugation. Cepsul Capsule is kind ofsime layer, which cavers the outside q ofthe cell wall. ‘They are composed of a thick polysaccharide. 4 itis Used to stick osls together and works as a food resene. It protects the cell from dryness and from chemicals, i : Reproduction in Bacteria: : sion ans Binary 2 reproduction n bacteria s many by cet cio "escrcin some cases tw baci i epratice y busing. Resprsion in bacteria i 5 respiration, Anaerobic bacteria: does not require oxygen for resp ‘Aerobie bactoria: require oxygen for respiration. famist ifferentiating badtefal — ‘Gram stelning bacteria-are-a method ofc bat ‘species into two large groups, which are basad on their chemical and physical properties oftheir cell wal. ‘Gram positive bacteria: Those bacteria when they are stained in gram stain results in purple colour. 20 gua sute resuns in pink colour Locomotion of bacteria: They move around by using thei locomotion and fagela nS Such as cla Phylogeny: All bacteria stem from a common ancestor and dwersied since, consequently possess diferent levels of evolutionary relatedness (see Bacterial phvla ard Timsline of valution) Metabolism: Different bacteria may have different metabolic ablities (see Mic-dbialmetabolism) Environment: Different bacteria thive in different environ such ashighow temperaturs and eat (a Extremoshiles) Morphology: There are many stuctural cifzrences between Soca uch ar colahepe Gran sah unto be lays) o* bilayer composition (see Bacterial cellar momholouies, Bactealcellstuctuce) Pathogenicity: Some bacteria are pathogenic to plants or ‘snimals (see Pathogenie Bacteria Subdivisions based on Gram stzfning Despite there being litte agreement on tha major subgroups ofthe Bacletia, Gram staining resulls were most commonly used a8 @

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