EVERWIN GROUP OF CBSE SCHOOLS
(Kolathur-Maduravoyal-Mathur-Perambur-Surapet-Madurantakam-Rajamangalam-Paruthipattu, Sembium)
DATE: 14.08.2025 BIOLOGY WORKSHEET CLASS: XII
1. Which of the following enzymes is not required for DNA replication in prokaryotes?
a) DNA ligase b) DNA polymerase III c) Helicase d) RNA polymerase
2. If a DNA strand has the sequence 5'-ATG CCG TAA-3', the complementary strand will be:
a) 3'-TAC GGC ATT-5' b) 5'-TAC GGC ATT-3'
c) 3'-UAC GGC AUU-5' d) 3'-ATG CCG TAA-5'
For the questions 3 and 4 given below choose any option
a) Both A and R are true, and R is the correct explanation of A.
b) Both A and R are true, but R is not the correct explanation of A.
c) A is true, but R is false.
d) Both assertion and reason are false
e) A is false, but R is true.
3. Assertion (A): DNA replication is semi-conservative.
Reason (R): In each newly formed DNA molecule, one strand is new while the other is parental.
4. Assertion (A): tRNA is called an adaptor molecule.
Reason (R): tRNA carries codons to the ribosome.
5. What is a nucleosome? Mention its components.
6. State the role of DNA polymerase in DNA replication. Why is it said to work in only one
direction?
7. Differentiate between exons and introns. How are they significant in eukaryotic gene
expression?
8. List any three differences between DNA and RNA.
9. A group of scientists were analyzing gene expression in E. coli. They found that when lactose
was present, a specific set of genes were expressed to produce enzymes required for its
metabolism. However, when lactose was absent, the same genes were turned off.
a) Name the type of gene regulation observed in E. coli.
b) How does the presence of an inducer like lactose affect the functioning of the operon?
10. Describe the structure of a transcription unit in DNA. Explain the process of transcription in
eukaryotes, highlighting the role of RNA polymerase and any post-transcriptional modifications.
