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Algorithm HIV

The document outlines a new HIV testing algorithm effective from March 1, 2021, which includes a three-test strategy instead of a tie-breaker test. It details the procedures for performing the One Step, First Response, and Uni-GOLD tests, including specific instructions for sample handling and result interpretation. The algorithm emphasizes the need for follow-up testing in cases of inconclusive results.

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yohannes woldemichael
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19 views3 pages

Algorithm HIV

The document outlines a new HIV testing algorithm effective from March 1, 2021, which includes a three-test strategy instead of a tie-breaker test. It details the procedures for performing the One Step, First Response, and Uni-GOLD tests, including specific instructions for sample handling and result interpretation. The algorithm emphasizes the need for follow-up testing in cases of inconclusive results.

Uploaded by

yohannes woldemichael
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
Available Formats
Download as DOCX, PDF, TXT or read online on Scribd

HIV Testing Algorithm-New

Doc Nr: SGHL/JA-5.5 Revision Nr: 0.0


Effective Date: 01-Mar-2021 Copy Nr: 1

HIV testing Algorithms

National HIV testing strategy (Three test Algorithm instead of the tie-breaker test
Perform One step (A1)

One step – NR
One step – R Report as
HIVNegative

Perform First
response- A2

A1 +Ve ,A2 +ve


A1+,A2-

Procedure to perform HIV testing:-


Perform UNI-GOLD (A3) A1+,A2-, HIV Inconclusive. Plan
1. One step – Kit Repeat A1 testing after 14 days
only

A1-, A2- Report as HIV Negative


A1+,A2+,A3-
A1+,A2+, A3+
HIV Inconclusive, plan
Report as HIV
testing after 14 days
Positive
HIV Testing Algorithm-New

Doc Nr: SGHL/JA-5.5 Revision Nr: 0.0


Effective Date: 01-Mar-2021 Copy Nr: 1

1. One Step

• Unseal the foil pouch and put the cassette on a clean, dry
and level surface
• Mark the specimen ID number on test cassette
• Add 1 drop of the specimen using the provided dropper
(or 30μl by transfer pipette) into port "S" of the cassette;

• Then add 1 drop of sample diluent into port "S"


immediately

• Wait and interpret the result between 15-20 minutes.

2. First Response-Kit

 Open the device pouch.


 Do not use the test device if the desiccant color has
changed from orange to green.
 Label the test device with the patient identification
number. Place the test device on a flat, clean and dry
surface.
 Using the specimen transfer device draw up the
serum/plasma up to 10 μlorwhole blood up to 20 μl marking
line on the specimen transfer device.
 Hold the assay buffer bottle vertically and add one drop of assay buffer to
the specimen well.
 Observe for development of purple colored lines in the results window.
HIV Testing Algorithm-New

Doc Nr: SGHL/JA-5.5 Revision Nr: 0.0


Effective Date: 01-Mar-2021 Copy Nr: 1

 Interpret test results at 15 minutes after adding assay buffer to the


specimen well.
 Do not interpret the test result after 25 minutes.

3. Uni Gold-Kit:-

 Collect the Venous or capillary blood


 Two drops of serum, plasma or whole blood is applied to the
sample port, followed by two drops of Wash Solution and
allowed to react.
 Allow the sample to fully absorb and add two (2) drops to the sample port.
 Read test results after 10 minutes but no later than 12
minutes incubation time.

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