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Chromatography Techniques Guide

Gas chromatography (GC) and high-pressure liquid chromatography (HPLC) are common types of chromatography used to separate mixtures. GC uses a gas carrier to vaporize samples that are then separated based on how compounds partition between the gas and a liquid stationary phase. HPLC uses liquid solvents at high pressure to separate compounds based on how they partition between the liquid mobile and stationary phases. Both techniques were developed in the early 20th century and are now widely used analytical methods in fields like chemistry and forensics.

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255 views16 pages

Chromatography Techniques Guide

Gas chromatography (GC) and high-pressure liquid chromatography (HPLC) are common types of chromatography used to separate mixtures. GC uses a gas carrier to vaporize samples that are then separated based on how compounds partition between the gas and a liquid stationary phase. HPLC uses liquid solvents at high pressure to separate compounds based on how they partition between the liquid mobile and stationary phases. Both techniques were developed in the early 20th century and are now widely used analytical methods in fields like chemistry and forensics.

Uploaded by

Criminegrology Tv
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© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
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Forensic

Instrumentation
Group 6
Sign In
GAS AND HIGH PRESSURE
LIQUID CHROMATOGRAPHY

INSERT THE TITLE


OF YOUR PRESENTATION HERE

GROUP 6 PRESENTATION [email protected]


Introduction
Definition X

Gas chromatography is a common type of


Gas Chromatography
chromatography used in analytical chemistry
for separating and analyzing compounds that
can be vaporized without decomposition.
Typical uses of GC include testing the purity
of a particular substance, or separating the
different components of a mixture.
High Pressure Liquid Chromatography X

High-performance liquid chromatography, formerly referred


Definition to as high-pressure liquid chromatography, is a technique in
analytical chemistry used to separate, identify, and quantify
each component in a mixture.
DEVELOPER X

The invention of GC is generally credited to British scien-


tists A. T. James and A. J. P. Martin for their 1952 paper on
gas-liquid partition chromatography (James and Martin
Who develop Gas 1952).
Chromatography
Their work gained the attention of the petroleum industry
and led to the improvement and widespread adoption of
modern GC.26 Aug 2019.
Developer X

Mikhail S. Tswett, 1903


Who developed Russian botanist
High Pressure
Liquid Chromatog-
raphy
1. The separation is hence accomplished by partitioning the
sample between the gas and a thin layer of a nonvolatile
liquid held on a solid support.

2. A sample containing the solutes is injected into a heated


block where it is immediately vaporized and swept as a plug
of vapor by the carrier gas stream into the column inlet.
How Gas C.
works? 3.The solutes are adsorbed by the stationary phase and then
desorbed by a fresh carrier gas.
The process is repeated in each plate as the sample is moved
toward the outlet.
4. Each solute will travel at its own rate through the col-
umn.
5. Their bands will separate into distinct zones depending
on the partition coefficients, and band spreading.

6. The solutes are eluted one after another in the increas-


ing order of their kd, and enter into a detector attached to
the exit end of the column.
7. The solutes are eluted one after another in the increasing or-
der of their kd, and enter into a detector attached to the exit
How Gas C. end of the column.
works?
8. Here they register a series of signals resulting from concen-
tration changes and rates of elution on the recorder as a plot of
time versus the composition of carrier gas stream.

9. The appearance time, height, width, and area of these peaks


can be measured to yield quantitative data.
How High Pres-
sure Liquid C.
works?
Introduction
Introduction
Diether Kane M. Santiago [email protected]

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