Spotters
Microscopy of Bacteria
1
Staphylococcus aureus ( GPC in clusters )
• It is a Gram stained smear of a clinical
specimen.
• Smear showing Gram positive spherical
shaped bacteria arranged in clusters, are seen.
• Clusters are due to cell division along 3 planes.
2
GPC in chains(Streptococci)
• It is a Gram stained smear of a sputum.
• Smear showing spherical shaped arranged in
chains, violet colored cocci with pus cells are
seen.
• Chains are due to cell division along 2 planes
only.
3
GPC+GNB
• It is a Gram stained smear of a clinical
specimen.
• Smear showing Gram positive cocci arranged
in clusters along with Gram negative bacilli are
seen
4
Clostridium tetani (GPB with spore)
• Smear showing Gram positive bacilli with
terminal spore.
• Drumstick appearance.
5
Streptococcus pneumoniae
• Smear showing flame shaped diplococci
arranged in pairs,
• violet colored with a halo around the
organisms are seen in the microscopic field.
6
Neisseria gonorrhoeae
• It is a Gram stained smear of a urethral
discharge.
• Smear showing intracellular kidney shaped
diplococci, pink colored organisms are seen in
the microscopic field.
7
Bacilli
Pus cells
Mycobacterium Tuberculosis
• It is a AFB stain of a sputum.
• Smear showing slender, rod shaped pink
colored organisms, beaded in appearance
with pus cells are seen in the microscopic
field.
8
Mycobacterium Leprae
• It is a AFB stain of a slit skin smear of a
hypopigmented patch.
• Smear showing rod shaped pink colored
organisms which are arranged in cigar bundle
pattern are seen in the microscopic field.
Spotters
Microscopy of Viruses
1
Rabies-Negri bodies
• It is a H & E stain of a clinical biopsy
specimen(brain).
• Smear showing intracytoplasmic eosinophilic
inclusions bodies are seen in the microscopic
field.
Spotters
Microscopic slides of Mycology
1
Candida species
• It is a Gram stain of a clinical specimen(nail,
sputum, urine & pus).
• Smear showing budding yeast oval cells (2-4μ),
violet colored organisms are seen in the
microscopic field.
2
Cryptococcus neoformans
• It is a negative staining(India ink preparation)
of a clinical specimen CSF.
• Smear showing capsulated yeast cells with or
without budding which are bright against dark
background are seen in the microscopic field.
3
Septate hyphae & conidia
Aspergillus fumigatus
• It is a LPCB stain of a clinical
specimen(sputum).
• Smear showing septate hypae, vesicle is
conical shaped and conidia arise from upper
third of vesicle are seen in the microscopic
field.
4
Septate hyphae & conidia
Aspergillus flavus
• It is a LPCB stain of a clinical
specimen(sputum).
• Smear showing septate hypae, vesicle is
globular shaped and conidia arise from entire
vesicle are seen in the microscopic field.
5
Septate hyphae & black conidia
Aspergillus niger
• It is a LPCB stain of a clinical
specimen(sputum).
• Smear showing septate hypae, vesicle is
globular shaped and conidia arise from entire
vesicle but conidia are black in colour are seen
in the microscopic field.
6
Asceptate hyphae Rhizoids Conidia
Rhizopus
• Smear showing broad aseptate hyaline hyphae
• Sporangiosphore with sporangium containing
sporangiospores
• Root like growth arising from hyphae called
rhizoid are seen in the microscopic field.
7
Asceptate hyphae Conidia
Mucor
• Smear showing broad aseptate hyaline
hyphae.
• Sporangiosphore with sporangium containing
sporangiospores are seen in the microscopic
field.
8
Conidia
Hyline septate hyphae
Penicillium Species
• Smear showing hyaline thin septate hyphae.
• Vesicle are absent, chains of conidia arise on
conidiophore which are in brush border
appearance are seen in the microscopic field.
9
Microconidia
Macroconidia
Trichophyton Mentagrophytes
• It is a LPCB stain of a clinical specimen(hair,
skin, nail) .
• Smear showing numerous microconida which
are round to pyrifom shaped along with few
macroconidia which are cigar shaped are seen
in the microscopic field.
10
Macroconidia
Epidermophyton Floccosum
• It is a LPCB stain of a clinical specimen(skin,
nail).
• Smear showing club or clavate shaped
macroconidia in clusters, 4-6 septa are seen in
the microscopic field and microconidia are
absent.
11
Macroconidia
Microconidia
Microsporum Gyspeum
• It is a LPCB stain of a clinical specimen(skin,
hair)
• Smear showing abundant macroconidia, thick
walled, spindle shaped, 4-6 septa, rounded
ends with rare microconidia are seen in the
microscpoic field.
Spotters
Microscopic slides of Parasitology
1
Gametocyte
Malaria falciparum
• It is a Leishman’s stain of peripheral thin
smear of blood.
• Smear showing Gematocytes which are
crescent shaped and or usually about 1.5
times the diameter of an red blood cell in
length are seen in the microscopic field.
2
Ring forms
Malaria falciparum
• It is a Leishman’s stain of peripheral thin
smear of blood.
• Smear showing ring forms(trophozoites) or
thin and delicate, measuring an average 1/5
diameter of the red blood cell are seen in the
microscopic field.
