signature=abcea710e8925522bb7987d7b1618edd,Energy transfer in purple bacterial photosynthetic units ...

最新推荐文章于 2022-08-30 16:08:05 发布

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最新推荐文章于 2022-08-30 16:08:05 发布

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文章探讨了野生型和pucBAabcem突变紫光菌Rps.palustris的内质膜（ICMs）光合作用特性，着重于不同光照条件下LH2复合物光谱改变对LH1到LH2的能量传递效率的影响。在室温和低温下，研究通过稳态吸收、荧光和飞秒时间分辨吸收，揭示了光谱特征变化对能量转移的重要作用。实验表明，在高光生长的ICMs中，经典B800-B850光谱的LH2积累会导致LH1到LH2的能量转移率显著增加，而在蓝移且B800与B850带几乎重叠的突变菌株中，观察不到这一过程。

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摘要：

Three photosynthetic membranes, called intra-cytoplasmic membranes (ICMs), from wild-type and the puc BA abce mutant of the purple phototrophic bacterium Rps. palustris were investigated using optical spectroscopy. The ICMs contain identical light-harvesting complex 1–reaction centers (LH1–RC) but have various spectral forms of light-harvesting complex 2 (LH2). Spectroscopic studies involving steady-state absorption, fluorescence, and femtosecond time-resolved absorption at room temperature and at 77K focused on inter-protein excitation energy transfer. The studies investigated how energy transfer is affected by altered spectral features of the LH2 complexes as those develop under growth at different light conditions. The study shows that LH1→LH2 excitation energy transfer is strongly affected if the LH2 complex alters its spectroscopic signature. The LH1→LH2 excitation energy transfer rate modeled with the Frster mechanism and kinetic simulations of transient absorption of the ICMs demonstrated that the transfer rate will be 2–3 times larger for ICMs accumulating LH2 complexes with the classical B800–850 spectral signature (grown in high light) compared to the ICMs from the same strain grown in low light. For the ICMs from the puc BA abce mutant, in which the B850 band of the LH2 complex is blue-shifted and almost degenerate with the B800 band, the LH1→LH2 excitation energy transfer was not observed nor predicted by calculations.

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