Immunization against a Saccharide Epitope Accelerates Clearance of Experimental Gonococcal Infection
Figure 5
Experimental design of active immunization with MAP1: Schematic representation.
Mice entering the diestrus phase of the estrous cycle were started on treatment (that day) with 0.5 mg. of water soluble 17β-estradiol (Sigma) given subcutaneously on each of three days; −2, 0 and +2 days (before, the day of and after inoculation) to prolong the estrus phase of the cycle and promote susceptibility to N. gonorrhoeae infection. Antibiotics ineffective against N. gonorrhoeae were also used to reduce competitive microflora.
doi: https://doi.org/10.1371/journal.ppat.1003559.g005