spi.bits_per_word = 16 OSError: [Errno 22] Invalid argument python-BaseException

时间: 2025-07-05 09:50:55 AIGC 浏览: 30
<think>好的,用户遇到了在Python中设置spi.bits_per_word时出现的OSError: [Errno 22] Invalid argument错误。我需要先理解这个错误的原因,然后找到解决方法。 首先,根据用户提供的引用信息,OSError 22通常与无效参数有关。比如文件名中的特殊字符会导致这个问题,但这里的情况是SPI设置中的bits_per_word参数。引用3提到,特殊字符在Windows文件名中不被允许,而SPI参数可能有类似的限制。 接下来,我应该考虑SPI配置中的参数限制。SPI的bits_per_word通常有特定的有效范围,比如常见的8位或16位。用户可能设置了不支持的值,比如9,这超出了硬件支持的范围,导致错误。 然后,检查硬件兼容性。不同的SPI设备支持的位数可能不同,需要确认设备文档。比如某些设备只支持8位,如果用户设置为其他值,就会出错。 另外,用户是否有足够的权限访问SPI设备?引用2提到文件路径的问题,但这里可能是设备节点权限问题。需要确保程序有权限访问/dev/spidevX.Y设备。 还有,SPI模式是否正确?比如mode参数是否设置为0-3之间的值,这也会影响其他参数的设置。 最后,交叉验证参数组合。某些参数组合可能不被硬件支持,比如高速度下设置某些位数可能无效。需要逐一检查参数是否兼容。 总结可能的解决步骤:检查bits_per_word的值是否有效,确认硬件支持,检查权限,验证SPI模式和其他参数,更新驱动或库版本,以及参考硬件文档。</think>要解决Python中设置`spi.bits_per_word`时出现的`OSError: [Errno 22] Invalid argument`错误,需按以下步骤排查: --- ### 1. **检查参数有效性** - **bits_per_word的取值范围**:SPI通信的`bits_per_word`通常支持$8$或$16$位等特定值。若设置非标准值(如$9$),会触发无效参数错误。 - **验证代码示例**: ```python import spidev spi = spidev.SpiDev() spi.open(0, 0) # 根据实际设备修改总线编号 spi.bits_per_word = 8 # 尝试标准值 ``` - 若尝试$8$位仍报错,需排查硬件兼容性[^3]。 --- ### 2. **确认硬件兼容性** - **设备文档查阅**:部分SPI硬件仅支持固定位数(如仅支持$8$位),需检查设备手册。 - **测试不同值**: ```python for bits in [8, 16]: try: spi.bits_per_word = bits print(f"成功设置bits_per_word={bits}") except OSError: print(f"不支持bits_per_word={bits}") ``` --- ### 3. **检查权限问题** - **设备节点权限**:确保用户对`/dev/spidev0.0`等设备节点有读写权限: ```bash sudo chmod 666 /dev/spidev0.0 # 临时授权 ``` - 若权限不足会导致参数设置失败[^2]。 --- ### 4. **验证SPI模式与参数组合** - **SPI模式冲突**:`mode`参数(如`spi.mode = 0`)需与其他参数(如时钟极性)兼容。 - **完整配置示例**: ```python spi.max_speed_hz = 1000000 # 降低时钟频率 spi.mode = 0 # 标准SPI模式 spi.bits_per_word = 8 ``` --- ### 5. **驱动或库版本问题** - **更新依赖库**:使用最新版`spidev`库: ```bash pip install --upgrade spidev ``` - **内核驱动支持**:某些SPI硬件需内核模块支持(如启用`spidev`驱动)。 --- ### 6. **参考硬件文档** - 部分嵌入式设备(如树莓派)对SPI参数有特殊限制,需查阅对应文档[^1]。 --- ### 总结流程图 ```mermaid graph TD A[报错OSError 22] --> B{检查bits_per_word值} B -->|无效| C[改为8/16等标准值] B -->|有效| D{硬件兼容性} D -->|不支持| E[更新硬件/驱动] D -->|支持| F{权限问题?} F -->|是| G[修改设备权限] F -->|否| H[检查SPI模式组合] ```
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D:\PyCharm 2024.3.4\plugins\python-ce\helpers\pycharm_display\datalore\display\supported_data_type.py:6: UserWarning: The NumPy module was reloaded (imported a second time). This can in some cases result in small but subtle issues and is discouraged. import numpy Traceback (most recent call last): File "E:\yolov5-master\test.py", line 5, in <module> model = YOLO('E:\yolov5-master\best.pt') # 替换为实际路径[^2] File "D:\python\lib\site-packages\ultralytics\models\yolo\model.py", line 23, in __init__ super().__init__(model=model, task=task, verbose=verbose) File "D:\python\lib\site-packages\ultralytics\engine\model.py", line 148, in __init__ self._load(model, task=task) File "D:\python\lib\site-packages\ultralytics\engine\model.py", line 290, in _load self.model, self.ckpt = attempt_load_one_weight(weights) File "D:\python\lib\site-packages\ultralytics\nn\tasks.py", line 1039, in attempt_load_one_weight ckpt, weight = torch_safe_load(weight) # load ckpt File "D:\python\lib\site-packages\ultralytics\nn\tasks.py", line 944, in torch_safe_load ckpt = torch.load(file, map_location="cpu") File "D:\python\lib\site-packages\ultralytics\utils\patches.py", line 86, in torch_load return _torch_load(*args, **kwargs) File "D:\python\lib\site-packages\torch\serialization.py", line 1065, in load with _open_file_like(f, 'rb') as opened_file: File "D:\python\lib\site-packages\torch\serialization.py", line 468, in _open_file_like return _open_file(name_or_buffer, mode) File "D:\python\lib\site-packages\torch\serialization.py", line 449, in __init__ super().__init__(open(name, mode)) OSError: [Errno 22] Invalid argument: 'E:\\yolov5-master\x08est.pt'

"""Append module search paths for third-party packages to sys.path. **************************************************************** * This module is automatically imported during initialization. * **************************************************************** This will append site-specific paths to the module search path. On Unix (including Mac OSX), it starts with sys.prefix and sys.exec_prefix (if different) and appends lib/python<version>/site-packages. On other platforms (such as Windows), it tries each of the prefixes directly, as well as with lib/site-packages appended. The resulting directories, if they exist, are appended to sys.path, and also inspected for path configuration files. If a file named "pyvenv.cfg" exists one directory above sys.executable, sys.prefix and sys.exec_prefix are set to that directory and it is also checked for site-packages (sys.base_prefix and sys.base_exec_prefix will always be the "real" prefixes of the Python installation). If "pyvenv.cfg" (a bootstrap configuration file) contains the key "include-system-site-packages" set to anything other than "false" (case-insensitive), the system-level prefixes will still also be searched for site-packages; otherwise they won't. All of the resulting site-specific directories, if they exist, are appended to sys.path, and also inspected for path configuration files. A path configuration file is a file whose name has the form .pth; its contents are additional directories (one per line) to be added to sys.path. Non-existing directories (or non-directories) are never added to sys.path; no directory is added to sys.path more than once. Blank lines and lines beginning with '#' are skipped. Lines starting with 'import' are executed. For example, suppose sys.prefix and sys.exec_prefix are set to /usr/local and there is a directory /usr/local/lib/python2.5/site-packages with three subdirectories, foo, bar and spam, and two path configuration files, foo.pth and bar.pth. Assume foo.pth contains the following: # foo package configuration foo bar bletch and bar.pth contains: # bar package configuration bar Then the following directories are added to sys.path, in this order: /usr/local/lib/python2.5/site-packages/bar /usr/local/lib/python2.5/site-packages/foo Note that bletch is omitted because it doesn't exist; bar precedes foo because bar.pth comes alphabetically before foo.pth; and spam is omitted because it is not mentioned in either path configuration file. The readline module is also automatically configured to enable completion for systems that support it. This can be overridden in sitecustomize, usercustomize or PYTHONSTARTUP. Starting Python in isolated mode (-I) disables automatic readline configuration. After these operations, an attempt is made to import a module named sitecustomize, which can perform arbitrary additional site-specific customizations. If this import fails with an ImportError exception, it is silently ignored. """ import sys import os import builtins import _sitebuiltins import io import stat import errno # Prefixes for site-packages; add additional prefixes like /usr/local here PREFIXES = [sys.prefix, sys.exec_prefix] # Enable per user site-packages directory # set it to False to disable the feature or True to force the feature ENABLE_USER_SITE = None # for distutils.commands.install # These values are initialized by the getuserbase() and getusersitepackages() # functions, through the main() function when Python starts. 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L = [] known_paths = set() for dir in sys.path: # Filter out duplicate paths (on case-insensitive file systems also # if they only differ in case); turn relative paths into absolute # paths. dir, dircase = makepath(dir) if dircase not in known_paths: L.append(dir) known_paths.add(dircase) sys.path[:] = L return known_paths def _init_pathinfo(): """Return a set containing all existing file system items from sys.path.""" d = set() for item in sys.path: try: if os.path.exists(item): _, itemcase = makepath(item) d.add(itemcase) except TypeError: continue return d def addpackage(sitedir, name, known_paths): """Process a .pth file within the site-packages directory: For each line in the file, either combine it with sitedir to a path and add that to known_paths, or execute it if it starts with 'import '. """ if known_paths is None: known_paths = _init_pathinfo() reset = True else: reset = False fullname = os.path.join(sitedir, name) try: st = os.lstat(fullname) except OSError: return if ((getattr(st, 'st_flags', 0) & stat.UF_HIDDEN) or (getattr(st, 'st_file_attributes', 0) & stat.FILE_ATTRIBUTE_HIDDEN)): _trace(f"Skipping hidden .pth file: {fullname!r}") return _trace(f"Processing .pth file: {fullname!r}") try: with io.open_code(fullname) as f: pth_content = f.read() except OSError: return try: # Accept BOM markers in .pth files as we do in source files # (Windows PowerShell 5.1 makes it hard to emit UTF-8 files without a BOM) pth_content = pth_content.decode("utf-8-sig") except UnicodeDecodeError: # Fallback to locale encoding for backward compatibility. # We will deprecate this fallback in the future. import locale pth_content = pth_content.decode(locale.getencoding()) _trace(f"Cannot read {fullname!r} as UTF-8. " f"Using fallback encoding {locale.getencoding()!r}") for n, line in enumerate(pth_content.splitlines(), 1): if line.startswith("#"): continue if line.strip() == "": continue try: if line.startswith(("import ", "import\t")): exec(line) continue line = line.rstrip() dir, dircase = makepath(sitedir, line) if dircase not in known_paths and os.path.exists(dir): sys.path.append(dir) known_paths.add(dircase) except Exception as exc: print(f"Error processing line {n:d} of {fullname}:\n", file=sys.stderr) import traceback for record in traceback.format_exception(exc): for line in record.splitlines(): print(' '+line, file=sys.stderr) print("\nRemainder of file ignored", file=sys.stderr) break if reset: known_paths = None return known_paths def addsitedir(sitedir, known_paths=None): """Add 'sitedir' argument to sys.path if missing and handle .pth files in 'sitedir'""" _trace(f"Adding directory: {sitedir!r}") if known_paths is None: known_paths = _init_pathinfo() reset = True else: reset = False sitedir, sitedircase = makepath(sitedir) if not sitedircase in known_paths: sys.path.append(sitedir) # Add path component known_paths.add(sitedircase) try: names = os.listdir(sitedir) except OSError: return names = [name for name in names if name.endswith(".pth") and not name.startswith(".")] for name in sorted(names): addpackage(sitedir, name, known_paths) if reset: known_paths = None return known_paths def check_enableusersite(): """Check if user site directory is safe for inclusion The function tests for the command line flag (including environment var), process uid/gid equal to effective uid/gid. None: Disabled for security reasons False: Disabled by user (command line option) True: Safe and enabled """ if sys.flags.no_user_site: return False if hasattr(os, "getuid") and hasattr(os, "geteuid"): # check process uid == effective uid if os.geteuid() != os.getuid(): return None if hasattr(os, "getgid") and hasattr(os, "getegid"): # check process gid == effective gid if os.getegid() != os.getgid(): return None return True # NOTE: sysconfig and it's dependencies are relatively large but site module # needs very limited part of them. # To speedup startup time, we have copy of them. # # See https://bugs.python.org/issue29585 # Copy of sysconfig._get_implementation() def _get_implementation(): return 'Python' # Copy of sysconfig._getuserbase() def _getuserbase(): env_base = os.environ.get("PYTHONUSERBASE", None) if env_base: return env_base # Emscripten, iOS, tvOS, VxWorks, WASI, and watchOS have no home directories if sys.platform in {"emscripten", "ios", "tvos", "vxworks", "wasi", "watchos"}: return None def joinuser(*args): return os.path.expanduser(os.path.join(*args)) if os.name == "nt": base = os.environ.get("APPDATA") or "~" return joinuser(base, _get_implementation()) if sys.platform == "darwin" and sys._framework: return joinuser("~", "Library", sys._framework, "%d.%d" % sys.version_info[:2]) return joinuser("~", ".local") # Same to sysconfig.get_path('purelib', os.name+'_user') def _get_path(userbase): version = sys.version_info if hasattr(sys, 'abiflags') and 't' in sys.abiflags: abi_thread = 't' else: abi_thread = '' implementation = _get_implementation() implementation_lower = implementation.lower() if os.name == 'nt': ver_nodot = sys.winver.replace('.', '') return f'{userbase}\\{implementation}{ver_nodot}\\site-packages' if sys.platform == 'darwin' and sys._framework: return f'{userbase}/lib/{implementation_lower}/site-packages' return f'{userbase}/lib/python{version[0]}.{version[1]}{abi_thread}/site-packages' def getuserbase(): """Returns the user base directory path. 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""" sitepackages = [] seen = set() if prefixes is None: prefixes = PREFIXES for prefix in prefixes: if not prefix or prefix in seen: continue seen.add(prefix) implementation = _get_implementation().lower() ver = sys.version_info if hasattr(sys, 'abiflags') and 't' in sys.abiflags: abi_thread = 't' else: abi_thread = '' if os.sep == '/': libdirs = [sys.platlibdir] if sys.platlibdir != "lib": libdirs.append("lib") for libdir in libdirs: path = os.path.join(prefix, libdir, f"{implementation}{ver[0]}.{ver[1]}{abi_thread}", "site-packages") sitepackages.append(path) else: sitepackages.append(prefix) sitepackages.append(os.path.join(prefix, "Lib", "site-packages")) return sitepackages def addsitepackages(known_paths, prefixes=None): """Add site-packages to sys.path""" _trace("Processing global site-packages") for sitedir in getsitepackages(prefixes): if os.path.isdir(sitedir): addsitedir(sitedir, known_paths) return known_paths def setquit(): """Define new builtins 'quit' and 'exit'. These are objects which make the interpreter exit when called. The repr of each object contains a hint at how it works. """ if os.sep == '\\': eof = 'Ctrl-Z plus Return' else: eof = 'Ctrl-D (i.e. EOF)' builtins.quit = _sitebuiltins.Quitter('quit', eof) builtins.exit = _sitebuiltins.Quitter('exit', eof) def setcopyright(): """Set 'copyright' and 'credits' in builtins""" builtins.copyright = _sitebuiltins._Printer("copyright", sys.copyright) builtins.credits = _sitebuiltins._Printer("credits", """\ Thanks to CWI, CNRI, BeOpen, Zope Corporation, the Python Software Foundation, and a cast of thousands for supporting Python development. See www.python.org for more information.""") files, dirs = [], [] # Not all modules are required to have a __file__ attribute. See # PEP 420 for more details. here = getattr(sys, '_stdlib_dir', None) if not here and hasattr(os, '__file__'): here = os.path.dirname(os.__file__) if here: files.extend(["LICENSE.txt", "LICENSE"]) dirs.extend([os.path.join(here, os.pardir), here, os.curdir]) builtins.license = _sitebuiltins._Printer( "license", "See https://www.python.org/psf/license/", files, dirs) def sethelper(): builtins.help = _sitebuiltins._Helper() def gethistoryfile(): """Check if the PYTHON_HISTORY environment variable is set and define it as the .python_history file. If PYTHON_HISTORY is not set, use the default .python_history file. """ if not sys.flags.ignore_environment: history = os.environ.get("PYTHON_HISTORY") if history: return history return os.path.join(os.path.expanduser('~'), '.python_history') def enablerlcompleter(): """Enable default readline configuration on interactive prompts, by registering a sys.__interactivehook__. 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This function is called automatically when this module is imported, unless the python interpreter was started with the -S flag. """ global ENABLE_USER_SITE orig_path = sys.path[:] known_paths = removeduppaths() if orig_path != sys.path: # removeduppaths() might make sys.path absolute. # fix __file__ and __cached__ of already imported modules too. abs_paths() known_paths = venv(known_paths) if ENABLE_USER_SITE is None: ENABLE_USER_SITE = check_enableusersite() known_paths = addusersitepackages(known_paths) known_paths = addsitepackages(known_paths) setquit() setcopyright() sethelper() if not sys.flags.isolated: enablerlcompleter() execsitecustomize() if ENABLE_USER_SITE: execusercustomize() # Prevent extending of sys.path when python was started with -S and # site is imported later. if not sys.flags.no_site: main() def _script(): help = """\ %s [--user-base] [--user-site] Without arguments print some useful information With arguments print the value of USER_BASE and/or USER_SITE separated by '%s'. Exit codes with --user-base or --user-site: 0 - user site directory is enabled 1 - user site directory is disabled by user 2 - user site directory is disabled by super user or for security reasons >2 - unknown error """ args = sys.argv[1:] if not args: user_base = getuserbase() user_site = getusersitepackages() print("sys.path = [") for dir in sys.path: print(" %r," % (dir,)) print("]") def exists(path): if path is not None and os.path.isdir(path): return "exists" else: return "doesn't exist" print(f"USER_BASE: {user_base!r} ({exists(user_base)})") print(f"USER_SITE: {user_site!r} ({exists(user_site)})") print(f"ENABLE_USER_SITE: {ENABLE_USER_SITE!r}") sys.exit(0) buffer = [] if '--user-base' in args: buffer.append(USER_BASE) if '--user-site' in args: buffer.append(USER_SITE) if buffer: print(os.pathsep.join(buffer)) if ENABLE_USER_SITE: sys.exit(0) elif ENABLE_USER_SITE is False: sys.exit(1) elif ENABLE_USER_SITE is None: sys.exit(2) else: sys.exit(3) else: import textwrap print(textwrap.dedent(help % (sys.argv[0], os.pathsep))) sys.exit(10) if __name__ == '__main__': _script()

以下代码中的,self.audio_queue.get()报错,导致线程中断,请问如何解决 from vosk import Model, KaldiRecognizer import sounddevice as sd import queue import threading import pystray from PIL import Image class VoiceWakeup: def __init__(self, wake_word="你好大力"): self.wake_word = wake_word self.model = Model(r"vosk-model-small-cn-0.22") # 需下载中文模型 # self.sample_rate = 16000 self.sample_rate = 44100 self.audio_queue = queue.Queue() self.is_listening = False def _audio_callback(self, indata, frames, time, status): self.audio_queue.put(bytes(indata)) def _process_audio(self): rec = KaldiRecognizer(self.model, self.sample_rate) while self.is_listening: data = self.audio_queue.get() if rec.AcceptWaveform(data): result = rec.Result() if self.wake_word in result: return True # 触发唤醒事件 return False def start_listen(self): self.is_listening = True with sd.RawInputStream(samplerate=self.sample_rate, blocksize=8000, dtype="int16", channels=1, callback=self._audio_callback): threading.Thread(target=self._process_audio).start() class TrayApp: def __init__(self): # 初始化语音唤醒模块 self.voice = VoiceWakeup(wake_word="启动助手") # 创建托盘图标 self.icon = pystray.Icon( name="VoiceTray", icon=Image.new("RGB", (64, 64), "blue"), menu=pystray.Menu( pystray.MenuItem("开启监听", self.start_listen), pystray.MenuItem("退出", self.on_exit) ) ) def on_wake(self): print(">>> 检测到唤醒词!") # 此处可扩展:显示通知/打开主界面等 self.icon.notify("语音指令已接收", "系统提示") def start_listen(self, icon, item): threading.Thread(target=self._listen_thread).start() def _listen_thread(self): if self.voice.start_listen(): self.on_wake() def on_exit(self, icon, item): self.voice.is_listening = False icon.stop() if __name__ == "__main__": TrayApp().icon.run()

PS D:\tdxbase> & D:/Python313/python.exe d:/tdxbase/DCF_Valuation_300595.py d:\tdxbase\DCF_Valuation_300595.py:17: SyntaxWarning: invalid escape sequence '\p' df = pd.read_csv(f'd:\tdxbase\pytdxdatabase\SZ_300595\SZ_{file_name}.day.csv', encoding='GBK', skiprows=1) d:\tdxbase\DCF_Valuation_300595.py:20: SyntaxWarning: invalid escape sequence '\Q' finance_df = pd.read_csv(f'd:\tdxbase\Quarterly Reports\SZ_300595\gpcw20260630.zip', parse_dates=['财报日期', '财报发布日期'], skiprows=1, encoding='gbk') Traceback (most recent call last): <unknown>:2: SyntaxWarning: invalid escape sequence '\p' <unknown>:1: SyntaxWarning: invalid escape sequence '\p' File "d:\tdxbase\DCF_Valuation_300595.py", line 102, in <module> df, finance_df = read_file(file_name) ~~~~~~~~~^^^^^^^^^^^ File "d:\tdxbase\DCF_Valuation_300595.py", line 17, in read_file df = pd.read_csv(f'd:\tdxbase\pytdxdatabase\SZ_300595\SZ_{file_name}.day.csv', encoding='GBK', skiprows=1) File "D:\Python313\Lib\site-packages\pandas\io\parsers\readers.py", line 1026, in read_csv return _read(filepath_or_buffer, kwds) File "D:\Python313\Lib\site-packages\pandas\io\parsers\readers.py", line 620, in _read parser = TextFileReader(filepath_or_buffer, **kwds) File "D:\Python313\Lib\site-packages\pandas\io\parsers\readers.py", line 1620, in __init__ self._engine = self._make_engine(f, self.engine) ~~~~~~~~~~~~~~~~~^^^^^^^^^^^^^^^^ File "D:\Python313\Lib\site-packages\pandas\io\parsers\readers.py", line 1880, in _make_engine self.handles = get_handle( ~~~~~~~~~~^ f, ^^ ...<6 lines>... storage_options=self.options.get("storage_options", None), ^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^ ) ^ File "D:\Python313\Lib\site-packages\pandas\io\common.py", line 873, in get_handle handle = open( handle, ...<3 lines>... newline="", ) OSError: [Errno 22] Invalid argument: 'd:\tdxbase\\pytdxdatabase\\SZ_300595\\SZ_300595.day.csv'

def send_file(self): """Send a file""" file_path = filedialog.askopenfilename() if not file_path: return if os.path.isdir(file_path): messagebox.showerror("Error", "Sending folders is not supported.") return target_ip = self.target_ip_entry.get() try: target_port = int(self.target_port_entry.get()) except ValueError: messagebox.showerror("Error", "Please enter a valid target port number.") return if not target_ip: messagebox.showwarning("Warning", "Please enter the target IP address.") return try: filename = os.path.basename(file_path) filesize = os.path.getsize(file_path) # Construct the file header header = f"FILE:{filename}:{filesize}".ljust(12).encode() # 创建新的连接 client_socket = socket.socket(socket.AF_INET, socket.SOCK_STREAM) client_socket.connect((target_ip, target_port)) client_socket.send(header) with open(file_path, 'rb') as f: while chunk := f.read(BUFFER_SIZE): client_socket.send(chunk) client_socket.close() self.update_history(f"File sent: {filename}") # 广播文件给所有连接的客户端,排除自身 with self.lock: connections_to_remove = [] for conn in self.client_connections: try: # 检查是否为自身连接 if conn.getpeername() != (target_ip, target_port): conn.send(header) f.seek(0) while chunk := f.read(BUFFER_SIZE): conn.send(chunk) except OSError as e: if e.errno == 32: # Broken pipe connections_to_remove.append(conn) self.update_history(f"Connection to client broken: {conn.getpeername()}", is_send=False) else: self.update_history(f"Error sending file to client: {str(e)}", is_send=False) # 移除断开的连接 for conn in connections_to_remove: if conn in self.client_connections: self.client_connections.remove(conn) except Exception as e: messagebox.showerror("Error", f"Failed to send the file: {str(e)}") def process_received_data(self, data, address): """Process the received data (general method)""" try: # Parse the protocol header header = data[:12].decode().strip() # Assume the header length is 12 bytes content = data[12:] if header.startswith("FILE"): parts = header.split(':') # File transfer protocol if len(parts) == 3: _, filename, filesize = header.split(':') self.save_file(content, filename, int(filesize), address) self.update_history(f"Received file: {filename} from {address}", is_send=False) else: self.update_history(f"Header format error: {header}", is_send=False) else: # Ordinary message message = content.decode('utf-8') self.update_history(f"{message}", is_send=False) except Exception as e: self.update_history(f"Data parsing error: {str(e)}", is_send=False) def save_file(self, data, filename, filesize, address): """Save the received file""" save_path = os.path.join(self.received_files_folder, filename) try: with open(save_path, 'wb') as f: f.write(data) remaining = filesize - len(data) while remaining > 0: data = self.client_socket.recv(BUFFER_SIZE) f.write(data) remaining -= len(data) self.update_history(f"File {filename} from {address} saved successfully.", is_send=False) except Exception as e: self.update_history(f"Error saving file {filename} from {address}: {str(e)}", is_send=False) def update_history(self, content, is_send=True): """Update the message history (thread-safe)""" def _update(): self.history_text.configure(state='normal') timestamp = datetime.now().strftime("[%H:%M:%S] ") tag = 'send' if is_send else 'receive' self.history_text.insert('end', timestamp + content + '\n', tag) self.history_text.configure(state='disabled') self.history_text.see(tk.END) # Update the interface through the main thread self.root.after(0, _update) def on_close(self): """关闭窗口时销毁资源""" self.server_running = False if self.server_socket: try: self.server_socket.close() except OSError: pass for conn in self.client_connections: try: conn.close() except OSError: pass self.root.destroy() def run(self): """Run the main program""" self.root.mainloop() if __name__ == "__main__": app = P2PApp() app.run() 修改代码:传输文件有问题

import cobra import pandas as pd import numpy as np import os, sys, argparse, logging from cobra.flux_analysis import flux_variability_analysis, single_gene_deletion # RIPTiDe import riptide # Statistics from sklearn.manifold import MDS from skbio.diversity import beta_diversity from skbio.stats.distance import permanova from sklearn.ensemble import RandomForestClassifier from sklearn.metrics import roc_auc_score # ========================= # Logging # ========================= def setup_logger(outdir): log_file = os.path.join(outdir, "pipeline.log") logging.basicConfig(level=logging.INFO, format="%(asctime)s [%(levelname)s %(message)s", handlers=[logging.FileHandler(log_file), logging.StreamHandler(sys.stdout)]) return log_file # ========================= # Core helpers # ========================= def read_sbml(path): model = cobra.io.read_sbml_model(path) logging.info(f"✅ Model loaded: {model.id}, Reactions={len(model.reactions)}, " f"Metabolites={len(model.metabolites)}, Genes={len(model.genes)}") return model def find_biomass_reaction(model): biomass_rxns = [r for r in model.reactions if "biomass" in r.id.lower() or "biomass" in r.name.lower()] if biomass_rxns: biomass = biomass_rxns[0] model.objective = biomass logging.info(f"✅ Biomass objective set: {biomass.id}") return biomass else: logging.warning("⚠️ No biomass reaction detected automatically") return None def find_atpm_reaction(model): candidates = [rid for rid in ["ATPM","R_ATPM","DM_atp_c_"] if rid in model.reactions] if candidates: rxn = model.reactions.get_by_id(candidates[0]) logging.info(f"ATPM reaction {rxn.id}: bounds {rxn.lower_bound},{rxn.upper_bound}") return rxn else: logging.warning("⚠️ No ATPM reaction detected") return None # ========================= # Media: S4 # ========================= def load_s4_gcb(path_s4): xls = pd.ExcelFile(path_s4) df = xls.parse("In Silico") out = df[["Reaction ID","LB (Equally Scaled)","LB (Molarity Scaled)"]].copy() out.columns = ["rxn_id","equally","molarity"] return out def build_media_from_s4(model, s4_df, scaling="equally", oxygen_override=None, log_prefix="S4"): lbcol = "equally" if scaling=="equally" else "molarity" applied = {} for _, row in s4_df.iterrows(): rid = row["rxn_id"] if rid not in model.reactions: continue if "_e" not in rid: continue rxn = model.reactions.get_by_id(rid) val = row[lbcol] if pd.isna(val): continue rxn.lower_bound = -float(val) rxn.upper_bound = 1000.0 applied[rid] = rxn.lower_bound # oxygen special if oxygen_override is not None and "EX_o2_e_" in model.reactions: model.reactions.EX_o2_e_.lower_bound = -float(oxygen_override) applied["EX_o2_e_"] = -float(oxygen_override) logging.info(f"{log_prefix} medium applied with {len(applied)} exchanges set") return applied # ========================= # Media: Custom GC broth + Vitox # ========================= def build_media_gc_vitox(model, scaling="molarity", oxygen_override=None): """ Custom GC broth + Vitox composition Values taken from proteose peptone analysis (mM) and Vitox supplement working concentrations. """ # Amino acids from proteose peptone (mM) base = { "EX_gly_e_":12.99, "EX_ala_L_e_":8.75, "EX_glu_L_e_":8.16, "EX_leu_L_e_":6.40, "EX_asp_L_e_":5.75, "EX_val_L_e_":4.48, "EX_pro_L_e_":4.95, "EX_lys_L_e_":4.31, "EX_arg_L_e_":3.70, "EX_ile_L_e_":3.66, "EX_phe_L_e_":3.18, "EX_thr_L_e_":1.89, "EX_ser_L_e_":2.28, "EX_his_L_e_":1.26, "EX_met_L_e_":1.31, "EX_tyr_L_e_":1.32, "EX_cys_L_e_":0.37, "EX_asn_L_e_":0.34, "EX_trp_L_e_":0.22 } # Ions (mM) base.update({ "EX_na1_e_":24.87,"EX_cl_e_":10.75,"EX_pi_e_":2.38,"EX_k_e_":5.05,"EX_so4_e_":0.58, "EX_mg2_e_":0.064,"EX_ca2_e_":0.049,"EX_fe3_e_":0.006 }) # Extra carbon source from starch (as glucose) base["EX_glc_D_e_"] = 2.8 # Vitox additions (approx mM) vitox = { "EX_cbl1_e_":0.000074, "EX_ade_e_":0.037, "EX_gln_L_e_":0.684, "EX_gua_e_":0.020, "EX_paba_e_":0.009, "EX_cyst_L_e_":0.092, "EX_nad_e_":0.003, "EX_thmpp_e_":0.003, "EX_fe3_e_":0.036, "EX_thm_e_":0.089, "EX_cys_L_e_":1.54, "EX_glc_D_e_":5.56 } # Merge media = {**base, **vitox} applied = {} for ex_id,val in media.items(): if ex_id not in model.reactions: continue rxn = model.reactions.get_by_id(ex_id) rxn.lower_bound = -float(val) rxn.upper_bound = 1000.0 applied[ex_id] = -float(val) if oxygen_override is not None and "EX_o2_e_" in model.reactions: model.reactions.EX_o2_e_.lower_bound = -float(oxygen_override) applied["EX_o2_e_"] = -float(oxygen_override) logging.info(f"GC+Vitox medium applied with {len(applied)} exchanges set") return applied # ========================= # Core analyses # ========================= def run_fba(model, biomass): sol = model.optimize() mu = sol.objective_value if mu>0: dt = np.log(2)*60.0/mu else: dt = np.inf logging.info(f"FBA: mu={mu:.4f} 1/h, DT={dt:.2f} min") return mu, dt, sol def run_fva(model): fva_res = flux_variability_analysis(model, fraction_of_optimum=1.0) return fva_res def run_sgd(model): sgd_res = single_gene_deletion(model) return sgd_res # ========================= # RIPTiDe contextualization # ========================= def contextualize_with_riptide(model, expr_path, outdir): """ Contextualize model using RIPTiDe with transcriptomic data - Exchange bounds: ±10 (O2 ±20) - maxfit_contextualize(min_frac=0.1, max_frac=0.8, n=1000) - Sampling n=500 """ # Load transcriptomics (assume gene ID → TPM/FPKM) expr = pd.read_csv(expr_path, index_col=0, squeeze=True) logging.info(f"Loaded expression file {expr_path} with {len(expr)} entries") # Set uniform exchange bounds ±10, except oxygen ±20,先统一设置 exchange bounds (±10, O₂=±20) for rxn in model.exchanges: if "o2" in rxn.id.lower(): rxn.lower_bound = -20 else: rxn.lower_bound = -10 rxn.upper_bound = 1000.0 # Contextualize,筛选与表达数据一致的代谢子网 ctx = riptide.maxfit_contextualize(model, expr, min_frac=0.1, max_frac=0.8, n=1000) logging.info("RIPTiDe contextualization complete") # Sampling,做 500 次 flux 抽样 samples = riptide.sample(ctx, n=500) df = pd.DataFrame(samples, columns=[r.id for r in ctx.reactions]) df.to_csv(os.path.join(outdir, "riptide_samples.tsv"), sep="\t") logging.info(f"RIPTiDe sampling complete, saved {df.shape} flux profiles") return df # ========================= # Downstream statistics # ========================= def analyze_flux_profiles(df, metadata, outdir): """ Perform: - Bray–Curtis NMDS - PERMANOVA - RandomForest classification with AUC """ # Compute Bray–Curtis dissimilarity,计算 Bray–Curtis 距离矩阵 dist = beta_diversity("braycurtis", df.values, ids=df.index) # 使用 MDS 实现 NMDS nmds = MDS(n_components=2, metric=False, max_iter=3000, eps=1e-12, dissimilarity='precomputed', random_state=42, n_jobs=1, n_init=1) # NMDS ordination (2D),用 NMDS 可视化群落代谢流差异 coords_array = nmds.fit_transform(dist.data) coords = pd.DataFrame(coords_array, index=df.index, columns=["NMDS1", "NMDS2"]) coords.to_csv(os.path.join(outdir, "nmds_coords.tsv"), sep="\t") # PERMANOVA,用 PERMANOVA 检验组间差异是否显著 meta = pd.Series(metadata, index=df.index) perma_res = permanova(dist, meta, permutations=999) with open(os.path.join(outdir,"permanova.txt"),"w") as f: f.write(str(perma_res)) # RandomForest classification,用 随机森林 分类 flux profiles,输出 AUC 评估判别力 clf = RandomForestClassifier(n_estimators=1500, max_features=20, random_state=42) y = meta.values clf.fit(df.values, y) # AUC (binary assumed) if len(set(y))==2: probs = clf.predict_proba(df.values)[:,1] auc = roc_auc_score(y, probs) else: auc = np.nan with open(os.path.join(outdir,"rf_auc.txt"),"w") as f: f.write(f"AUC={auc}\n") logging.info("Downstream statistics complete") # ========================= # Main # ========================= def main(): ap = argparse.ArgumentParser() ap.add_argument("--sbml", default="NGO_557.sbml") # 默认模型路径 ap.add_argument("--s4", default="msystems.01265-22-s0004.xlsx") ap.add_argument("--expr", help="Transcriptomics file for RIPTiDe") ap.add_argument("--mode", choices=["s4","custom_gc_vitox"], default="s4") ap.add_argument("--scaling", choices=["equally","molarity"], default="molarity") ap.add_argument("--oxygen_override", type=float, default=None) ap.add_argument("--outdir", default="results_out") # 默认输出文件夹 args = ap.parse_args() os.makedirs(args.outdir,exist_ok=True) setup_logger(args.outdir) model = read_sbml(args.sbml) biomass = find_biomass_reaction(model) find_atpm_reaction(model) # Media setup if args.mode=="s4": if not args.s4: logging.error("S4 path required for mode s4") return s4df = load_s4_gcb(args.s4) build_media_from_s4(model,s4df,args.scaling,oxygen_override=args.oxygen_override) elif args.mode=="custom_gc_vitox": build_media_gc_vitox(model,scaling=args.scaling,oxygen_override=args.oxygen_override) # Analyses mu,dt,sol = run_fba(model,biomass) fva = run_fva(model); fva.to_csv(os.path.join(args.outdir,"fva.tsv"),sep="\t") sgd = run_sgd(model); sgd.to_csv(os.path.join(args.outdir,"sgd.tsv"),sep="\t") # RIPTiDe if transcriptomics provided if args.expr: flux_df = contextualize_with_riptide(model, args.expr, args.outdir) # downstream stats skeleton (needs metadata, here dummy example) metadata = {idx: ("Group1" if i < len(flux_df)//2 else "Group2") for i,idx in enumerate(flux_df.index)} analyze_flux_profiles(flux_df, metadata, args.outdir) logging.info("Pipeline complete") if __name__=="__main__": main() 这段代码的报错为:D:\python+pycharm\Miniconda\envs\skbio-python39\python.exe D:/实验室/python/25.8.22.py Traceback (most recent call last): File "D:\实验室\python\25.8.22.py", line 283, in <module> main() File "D:\实验室\python\25.8.22.py", line 253, in main model = read_sbml(args.sbml) File "D:\实验室\python\25.8.22.py", line 33, in read_sbml model = cobra.io.read_sbml_model(path) File "D:\python+pycharm\Miniconda\envs\skbio-python39\lib\site-packages\cobra\io\sbml.py", line 460, in read_sbml_model raise e File "D:\python+pycharm\Miniconda\envs\skbio-python39\lib\site-packages\cobra\io\sbml.py", line 457, in read_sbml_model doc = _get_doc_from_filename(filename) File "D:\python+pycharm\Miniconda\envs\skbio-python39\lib\site-packages\cobra\io\sbml.py", line 504, in _get_doc_from_filename raise IOError( OSError: The file with 'NGO_557.sbml' does not exist, or is not an SBML string. Provide the path to an existing SBML file or a valid SBML string representation: 进程已结束,退出代码1

报错 (mask3d) yc@DESKTOP-BBCG9DJ:~/workspace/Mask3D-main$ pip install torch_scatter-2.1.0+pt112cu113-cp310-cp310-linux_x86_64.whl Processing ./torch_scatter-2.1.0+pt112cu113-cp310-cp310-linux_x86_64.whl Installing collected packages: torch-scatter Successfully installed torch-scatter-2.1.0+pt112cu113 (mask3d) yc@DESKTOP-BBCG9DJ:~/workspace/Mask3D-main$ python main_instance_segmentation.py general.experiment_name="validation" general.project_name="stpls3d" data/datasets=stpls3d general.num_targets=15 data.num_labels=15 data.voxel_size=0.333 data.num_workers=10 data.cache_data=true data.cropping_v1=false general.reps_per_epoch=100 model.num_queries=${CURR_QUERY} general.on_crops=true model.config.backbone._target_=models.Res16UNet18B data.crop_length=${CURR_SIZE} general.eval_inner_core=50.0 Traceback (most recent call last): File "/mnt/h/workspace/Mask3D-main/main_instance_segmentation.py", line 8, in <module> from trainer.trainer import InstanceSegmentation, RegularCheckpointing File "/mnt/h/workspace/Mask3D-main/trainer/trainer.py", line 9, in <module> from torch_scatter import scatter_mean File "/home/yc/anaconda3/envs/mask3d/lib/python3.10/site-packages/torch_scatter/__init__.py", line 16, in <module> torch.ops.load_library(spec.origin) File "/home/yc/anaconda3/envs/mask3d/lib/python3.10/site-packages/torch/_ops.py", line 1295, in load_library ctypes.CDLL(path) File "/home/yc/anaconda3/envs/mask3d/lib/python3.10/ctypes/__init__.py", line 374, in __init__ self._handle = _dlopen(self._name, mode) OSError: /home/yc/anaconda3/envs/mask3d/lib/python3.10/site-packages/torch_scatter/_version_cuda.so: undefined symbol: _ZN3c1017RegisterOperatorsD1Ev

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