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Glucose GOD - PAP: O H 2 Gluconate O H 2 O Glucose + + +

This document summarizes an enzymatic colorimetric method for determining glucose concentration in serum, plasma, or CSF samples. Glucose is oxidized by glucose oxidase to form hydrogen peroxide, which then reacts with phenol and 4-aminoantipyrine in the presence of peroxidase to form a colored product. The intensity of the color is proportional to the glucose concentration. Expected fasting serum or plasma glucose concentrations are 3.05-6.11 mmol/L. The method is linear up to 27.75 mmol/L. Quality controls and safety precautions are also provided.

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256 views1 page

Glucose GOD - PAP: O H 2 Gluconate O H 2 O Glucose + + +

This document summarizes an enzymatic colorimetric method for determining glucose concentration in serum, plasma, or CSF samples. Glucose is oxidized by glucose oxidase to form hydrogen peroxide, which then reacts with phenol and 4-aminoantipyrine in the presence of peroxidase to form a colored product. The intensity of the color is proportional to the glucose concentration. Expected fasting serum or plasma glucose concentrations are 3.05-6.11 mmol/L. The method is linear up to 27.75 mmol/L. Quality controls and safety precautions are also provided.

Uploaded by

Anonymous Aaubnb
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© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
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Glucose GOD - PAP

Enzymatic - colorimetric method /liquid


Cat.No. 101-0442

Size: 4x250 ml

PRINCIPLE:

NOTE:

Glucose is determined after enzymatic oxidation in the presence of


glucose oxidase (GOD). The formed H2O2 reacts under catalysis
of peroxidase (POD) with phenol and 4-amino-antipyrine to form
quinoneimine. The intensity of the colour is proportional to the
glucose concetration in the sample.

Volumes can be proportionally changed.

CALCULATION:
Asample

gluconate + 2H 2 O 2
glucose + O 2 + 2H 2 O GOD

Astandard

guinoneimine + 4H 2 O
2H 2 O 2 + phenol + 4 amino antipyrine GOD

EXPECTED VALUES:

SAMPLE:

Serum, plasma or CSF.


Glucose is stable for 3 days at +2 oC to +8 oC.

Serum, plasma ( fasting )

REAGENTS:
1.

2.

92 mmol/L
0.3 mmol/L
15000 U/L
1000 U/L
2.6 mmol/L

up to 27.75 mmol/L (500 mg/dL)

QUALITY CONTROL:
CONTRO-N

20 x 5 ml

Cat. No. 101-0083

CONTRO-P

20 x 5 ml

Cat. No. 101-0084

NOTE:
Standard concentration see on the vial label

1.
2.

PREPARATION OF REAGENTS
Liquid reagent, ready to use.
This reagent is stable up to the date of exparation at +2 oC to +8
o
C. Avoid direct sunlight.

3.
4.

PROCEDURE:
Wavelength:

505 nm, Hg 546 nm (490 - 550 nm)

Cuvette:

1 cm light path
25 C, 30 oC, 37 oC

Zero:

reagent blank

Plasma or serum

Pipette into test


tubes

Sample with glucoese concentration > 500 mg/dL has to be


diluted 1:2 with physiological solution ( result x 2 ).
Do not interfere: Hemoglobin (4g/L); Bilirubin (20 mg/L);
Creatinine (100 mg/L), Galactose (1g/L) and EDTA (2 g/L)
Reagent contains sodium azide as stabilizer. Do not swalow.
Avoid contact with the skin and mucous membranes.
Reagent 1 contains phenol, which is poisonous and caustic.
Do not swallow, and avoid contact with skin. If solution
comes into contact with skin, flush immediately with
polyethylene glycol 400 or, lacking this, with large
quantities of water.

Temperature:

1.

3.05 - 6.11 mmol/L (55 - 110mg/dL)

LINEARITY:

Reagent
Tris buffer, pH 7.4
Phenol
GOD
POD
4-amino-ahenazone
Standard
Glucose

stand.conc. = Glucose conc.

Reagent
blank

Standard

Sample

Standard

10 l

Sample

10 l

REFERENCES:
1.
2.
3.

Trinder P.A.; An. Clin. Biochem. 6, 24 (1969).


Dingeon, B. Ann. Biol. Clin. 33,3 (1975).
Lott, J.A. Clin. Chem. 21, 1754 (1975)

Working
1000 l
1000 l
1000 l
reagent
Mix and incubate for 30 min. at room temperature or 10 min. at
37oC. Avoid exposure to direct sunlight. Measure the absorbance
of the standard and the sample against the reagent blank within
30 minutes.

EN0782MI/02

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