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3 Acid Dissociation Constant of Methyl Red

This document discusses determining the acid dissociation constant (pKa) of methyl red using spectrophotometry. A spectrophotometer measures the absorption of different wavelengths of light by a substance. Methyl red exists in both acidic and basic forms depending on the solution's pH. The experiment measures the absorbances of methyl red solutions at different pH levels. Using the Beer-Lambert law and Henderson-Hasselbach equation, the relative amounts of the acidic and basic forms can be calculated to determine methyl red's pKa.

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Ercille Mae Oraiz Pacamo
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149 views2 pages

3 Acid Dissociation Constant of Methyl Red

This document discusses determining the acid dissociation constant (pKa) of methyl red using spectrophotometry. A spectrophotometer measures the absorption of different wavelengths of light by a substance. Methyl red exists in both acidic and basic forms depending on the solution's pH. The experiment measures the absorbances of methyl red solutions at different pH levels. Using the Beer-Lambert law and Henderson-Hasselbach equation, the relative amounts of the acidic and basic forms can be calculated to determine methyl red's pKa.

Uploaded by

Ercille Mae Oraiz Pacamo
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
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Download as DOCX, PDF, TXT or read online on Scribd
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3 Acid Dissociation Constant of Methyl Red

Christian Paolo Asequia, Ercille Mae Pacamo, Rene Susette Ann Pontillas, Mariza
Silagan
[email protected], email ad nimo Ert, [email protected], [email protected]
KEYWORDS :

ABSTRACT:

INTRODUCTION
A spectrophotometer, an instrument used
to determine the intensity of various wavelengths
in a spectrum of light, separates light into its
separate colors because each color of light has a
distinct wavelength. The spectrophotometer can
shine a narrow band of wavelengths of light on a
sample of substance and then measure how
much light is absorbed by the sample. Different
colored substances absorb varying amounts of
specific wavelength of light therefore a
spectrophotometer can be used to measure how
much of a substance is present in a sample. [1]

Figure2AcidandBasicformsofMethylRed

The H+ (or H3O+) in solution determines the ratio


of MR- and HMR in the following equation

M R

At pH 4.2 the solution is red and pH 6.2 the


solution is yellow. For any material that absorbs
light, according to Beer-Lambert Law, absorption
Aabs is given as
||=Lc (2)

Figure1Spectrophotometer

An acid dissociation constant, K a, is a


quantitative measure of the strength of an acid
solution. The experiment involves the direct
spectrophotometer determination of the acid
dissociation of methyl red (MR).
Methyl red is a weak acid in which its
color in aqueous solution depends on the pH.
Figure 2 shows the acidic (HMR) and basic (MR -)
forms of methyl red. The acid form is a zwitterion
in solution. It is considered to resonate with an
electronic
structure
with
an
electronic
configuration somewhere between the two
extreme forms

where c is the concentration, L is the path length,


and is the coefficient of absorption which
depends in the type of substance that absorbs
and to the wavelength of absorption. [2]
Equations 3 and 4 show that the observed
absorbances at the absorption maximas are the
simple additive sums of the absorbances due to
HMR and MR-

M R

A , M R
A A =a A , HMR [ HMR ] +a

M R

B, M R
A B =a B , HMR [ HMR ] +a
Thus the relative amounts of HMR and MR - in
solution can be calculated. pKa can then be
obtained
using
the
Henderson-Hasselbach
equation


MR

p K a= pH log

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