1.
0 TITLE
Determination of Ammonia-Nitrogen and Nitrate-Nitrogen in Water and Wastewater
2.0 OBJECTIVE
To determine ammonia-nitrogen and nitrate-nitrogen in water and wastewater
3.0 INTRODUCTION
In ammonia-nitrogen test, ammonia compounds combine with chlorine to form
monochloride. Monochloride reacts with salicylate to form -aminosalicylate. The -
aminosalicylate is o!idi"ed in the presence of a sodium nitroprusside catalyst to form a
blue-colored compound. The blue color is mas#ed by the yellow color from the e!cess
reagent present to gi$e a final green-colored solution. Test results are measured at %
nm. Meanwhile, in nitrate-nitrogen test, cadmium metal reduces nitrates in the sample to
nitrite. The nitrite ion reacts in an acidic medium with sulfanilic acid to form an
intermediate dia"onium salt. The salt couples with gentisic acid to form an amber colored
solution. Test results are measured at &'( nm.
4.0 MATERIALS & METHODOLOGY
Ammonia )yanurate *eagent +owder +illows-,
Ammonia -alicylate *eagent +owder +illows-,
Nitra.er Nitrate *eagent +owder +illows-/
0A)0 -pectrophotometer D*1,&(( 2 D*1,3((
*ounded1-4uare sample cell, /( m5
Measuring cylinder, , m5
6ea#er, ( Ml
Ammoni Ni!"o#$n %M$!&o' (1))* 0.01 !o 0.)0 m#+L NH
3
,N-
/. Touch H.& /"o#"m0. -elect program 3() N* Ammoni* S1i.. Touch S!"!.
,. 7ill a round sample cell to the /( m5 mar# with sample.
'. 7ill another round sample cell to the /( m5 mar# with deioni"ed water 8the
blan#9.
&. Add the contents of one Ammonia -alicylate +owder +illow to each cell. -topper
and sha#e to dissol$e the powder.
. Touch the timer icon. Touch O2. A three-minute reaction period will begin.
%. When the timer beeps, add the contents of one Ammonia )yanurate *eagent
+owder +illow. -topper and sha#e to dissol$e the reagent.
:. Touch the timer icon. Touch O2. A /-minute reaction period will begin.
;A green color will de$elop if ammonia-nitrogen is present.
3. When the timer beeps, place the blan# into the cell holder.
<. Touch 3$"o. The display will show= 0.00 m#+L NH
3
,N.
/(. Wipe the sample and place it into the cell holder.
//. Touch R$'. *esults will appear in mg15 N0
'
-N.
Ni!"!$ %M$!&o' (141* MR 0.1 !o 10.0 m#+L NO
3,
,N-
/. Touch H.& /"o#"m0. -elect program 3)3 N* Ni!"!$ MR. Touch S!"!.
,. 7ill a round sample cell with /( m5 of sample.
'. Add the contents of one Nitra.er Nitrate *eagent +owder +illow 8the prepared
sample9 and cap the cell.
&. Touch the timer icon. Touch >?. A one-minute reaction period will begin. -ha#e
the cell $igorously until the timer beeps.
. When the timer beeps, touch the timer icon. Touch O2. A fi$e-minute reaction
period will begin.
;An amber color will de$elop if nitrate is present.
%. When the timer beeps, fill a second round sample cell with /( m5 of sample 8the
blan#9.
:. +lace the blan# into the cell holder.
3. Touch 3$"o. The display will show= 0.0 m#+L NO
3,
,N.
<. +lace the prepared sample into the cell holder. Touch *ead. *esults will appear in
mg15 N>
'
-
-N.
).0 RESULTS & DISCUSSIONS
-ample 8different
sources9
N0
'
-N, mg15 N>
'
-
-N, mg15
@our discussion may include the following 4uestions=
/. )ompare and e!plain the $alue of ammonia nitrogen and nitrate ion nitrogen in
the difference sources of sample.
,. Identify the sources of ammonia nitrogen in water and wastewater.
'. A!plain the biochemical reactions that can remo$e nitrate from water.
5.0 RE6ERENCES
0oward, -. +., Donald, *. *. B Ceorge, T. /<3. An$ironmental Angineering. McCraw-
0ill. -ingapore.
?asmawati, M. B 5ee, ?. ?. ,((:. Methods of Analysis for Water and Wastewater.
DiTM. -hah Alam.
D*1,&(( -pectrophotometer +rocedure Manual. ,((&. 0ach )ompany. D-A.
