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No. P/V Wavelength Nm. Absorbance Description 1 244 0.785

This document contains information from a UV-Vis spectroscopy experiment measuring the absorbance of vitamin C (ascorbic acid) samples and standards at different wavelengths. It includes a standard curve generated from absorbance measurements of 5 standards with concentrations ranging from 0-20 ppm. The standard curve has an R2 value of 0.99926, indicating a very strong linear correlation between concentration and absorbance. Absorbance values are also reported for 3 vitamin C samples labeled Vitacimin (12), Vitacimin (12b), and Vitacimin (20).

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Larasati Denaputri
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23 views

No. P/V Wavelength Nm. Absorbance Description 1 244 0.785

This document contains information from a UV-Vis spectroscopy experiment measuring the absorbance of vitamin C (ascorbic acid) samples and standards at different wavelengths. It includes a standard curve generated from absorbance measurements of 5 standards with concentrations ranging from 0-20 ppm. The standard curve has an R2 value of 0.99926, indicating a very strong linear correlation between concentration and absorbance. Absorbance values are also reported for 3 vitamin C samples labeled Vitacimin (12), Vitacimin (12b), and Vitacimin (20).

Uploaded by

Larasati Denaputri
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
Available Formats
Download as PDF, TXT or read online on Scribd
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Abs.

Abs.

Wavelength nm.
Absorbance
Description
244
0.785

Absorbance
0.423
0.424
1.015

265.00
nm.

285.00

20.000

Sample
Vitacimin (12)
Vitacimin (12b)
Vitacimin (20)

0.000
245.00

Standard Curve

P/V
1

1.450

No.

320.00

15.000

270.00
nm.

1.000

1.550

10.000
Conc. (mg/l)

0.000
220.00

0.500

5.000

0.500

-0.106
0.000

Correlation Coefficient r2 = 0.99928

3.100

1.000

Abs.

Sample
blanko
Standar 1
Standar 2
Standar 3
Standar 4

Standard
Standard
Standard
Standard
Standard

ppm [c]
0
8
12
16
20

Absorbance (A)
0.032
0.523
0.803
1.036
1.32

b
1
1
1
1
1

0
0.065375
0.066916667
0.06475
0.066
0.052608333

Kurva Absorbansi Larutan Standar Asam


Askorbat pada Berbagai Konsentrasi
1.4

Absorbance

1.2
1
0.8

y = 0.0642x + 0.0239
R = 0.99926

0.6
0.4
0.2
0
0

10

15

Concentra-on (ppm)

20

25

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