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Ulva Lactuca Ethanol Production Methodology

The document outlines the materials, methodology, and procedures for an experiment involving the pretreatment of Ulva Lactuca seaweed biomass and its conversion to ethanol. Key steps include pretreatment of the biomass using acid hydrolysis and subcritical water extraction, saccharification using acid hydrolysis, fermentation using Zymomonas mobilis bacteria to convert sugars to ethanol, and analysis using HPLC and DLS. Safety, ethical, and environmental concerns regarding the use of chemicals, waste disposal, and impacts on wildlife are also addressed.

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84 views3 pages

Ulva Lactuca Ethanol Production Methodology

The document outlines the materials, methodology, and procedures for an experiment involving the pretreatment of Ulva Lactuca seaweed biomass and its conversion to ethanol. Key steps include pretreatment of the biomass using acid hydrolysis and subcritical water extraction, saccharification using acid hydrolysis, fermentation using Zymomonas mobilis bacteria to convert sugars to ethanol, and analysis using HPLC and DLS. Safety, ethical, and environmental concerns regarding the use of chemicals, waste disposal, and impacts on wildlife are also addressed.

Uploaded by

sg.com
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
Available Formats
Download as DOCX, PDF, TXT or read online on Scribd

Materials and Methodology Outline

4.1 Materials

4.1.1 Ulva Lactuca

.1.2 Reagents and Chemicals ( gagamiting chemicals para sa pretreament at analysis)

4.2 Experimental Set-Up

4.2.1 Experimental Flowchart

4.2.2Equipments used

4.3 Preparation of Material

4.3.1 Pretreatment of Ulva

4.3.1.1 Acid Hydrolysis

4.3.1 2 SWE

4.4 Experimental Procedure

4.4.1 Saccharification

4.4.2 Fermentation

4.5 Analysis

4.5.1 HPLC

4.5.2 DLS

4.6 Ethical, Environmental, Safety and Health Concern

4.6.1 Safety

4.6.1.1 Materials Safety

4.6.1.2 Hazard

4.6.2 Waste disposal (acid, alage and isip pa)

4.6.3 Other ethical concern

4.6.1 (sabhin lang na wala tayong ginamit na people, animals and wildlife)
Flowchart

Biomass Ethanol

Simultaneous Saccharification and Fermentation (SSF)

Pretreatment Acid Hydrolysis Fermentation Distillation and


(Subcritical (Saccharification) (Conversion of sugar Evaporation
Water) to ethanol)

Waste
Filter/Washing
Management Lignin

Residue
List of Materials

1. Ulva lactuca (Freeze dried mass)


2. Sulfuric Acid (Different concentrations)
3. Zymomonas mobilis culture

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