ROUTINE LABORATORY TESTS: CLINICAL HEMATOLOGY

AVAILABLE
VOLUME/ TIME OF HANDLING/ CUT OFF DAY
LABORATORY SPECIMEN COLLECTION/ STORAGE RUNNING DAY TAT FEE
QUANTITY COLLECTION TRANSPORT AND TIME
PROCEDURE

Complete Blood Count Collect in EDTA tube. Mix by

Blood 2 ml EDTA tube anytime -- Daily -- 2 hours 352.00
w/ Platelet Count inversion 10x

Collect in EDTA tube. Mix by

RBC / WBC Blood 2 ml EDTA tube anytime -- Daily -- 1 hour 180.00
inversion 10x

Hemoglobin/Hematocrit Collect in EDTA tube. Mix by

Blood 2 ml EDTA tube anytime -- Daily -- 2 hours 252.00
& Platelet inversion 10x

Collect in EDTA tube. Mix by

MCV / MCH / MCHC Blood 2 ml EDTA tube anytime -- Daily -- 1 hour 180.00
inversion 10x

Collect in EDTA tube, & finger prick

Peripheral Blood Smear Blood 2 slides smear anytime -- Daily -- 3 days 180.00
for direct smear

Collect in EDTA tube. Mix by

Reticulocyte Count Blood 2 ml EDTA tube anytime -- Daily -- 2 hours 140.00
inversion 10x

Erythrocyte Collect in EDTA tube. Mix by

Blood 2 ml EDTA tube anytime -- Daily -- 2 hours 200.00
Sedimentation Rate inversion 10x

Collect in heparinized syringe for

Transport
Heparinized transport in ice
Arterial Blood Gas 1-2 cc blood anytime immediately Daily -- 1 hour 1,500.00
blood
with ice
Note: Avoid bubbles

Blood / RH Typing Blood 2 ml EDTA tube anytime Collect in EDTA tube (violet top) -- Daily -- 2 hours 380.00

Du Typing Blood 2 ml EDTA tube anytime Collect in EDTA tube (violet top) -- Daily -- 2 hours 280.00

Coomb’s Test (Direct Collect in red plain tube. No

Blood 3-5 ml plain tube anytime -- Daily -- 2 hours 660.00
& Indirect) inversion.

Page 1 of 64
 AVAILABLE
VOLUME/ TIME OF HANDLING/ CUT OFF DAY
LABORATORY SPECIMEN COLLECTION/ STORAGE RUNNING DAY TAT FEE
QUANTITY COLLECTION TRANSPORT AND TIME
PROCEDURE

Collect in red plain tube. No

Coomb’s Test (Direct) Blood 3-5 ml plain tube anytime -- Daily -- 2 hours 330.00
inversion.

Coomb’s Test Collect in red plain tube. No

Blood 3-5 ml plain tube anytime -- Daily -- 2 hours 330.00
(Indirect) inversion.

Collect in red plain tube. No

Cross-Matching Blood 3-5 ml plain tube anytime -- Daily -- 2 hours 1,100.00
inversion.

Collect in blue top (with 3.2%

sodium citrate) plastic tube. Mix
by inversion 3-4x only
Transport
Prothrombin Blood 18 ml blue top anytime Daily -- 2 hours 550.00
immediately
Centrifuge and separate plasma.
Processed within 4 hours after
collection.

Collect in blue top (with 3.2%

sodium citrate) plastic tube. Mix
by inversion 3-4x only
Partial Transport
Blood 18 ml blue top anytime Daily -- 2 hours 550.00
Thromboplastin Time immediately
Centrifuge and separate plasma.
Processed within 4 hours after
collection.
Drops of blood Collect in EDTA tube. Mix by
Bleeding Time Blood anytime -- Daily -- 1 hour 140.00
through finger prick inversion 10x

Drops of blood Collect in EDTA tube. Mix by

Clotting Time Blood anytime -- Daily -- 1 hour 170.00
through finger prick inversion 10x

Page 2 of 64
ROUTINE LABORATORY TESTS: CLINICAL CHEMISTRY
AVAILABLE
VOLUME/ TIME OF HANDLING/ CUT OFF DAY
LABORATORY SPECIMEN COLLECTION/ STORAGE RUNNING DAY TAT FEE
QUANTITY COLLECTION TRANSPORT AND TIME
PROCEDURE
*at least 8 hours Fasting
Monday to Friday (8am-
5pm); No OPD on
Collect in red top (plain), or yellow
Saturdays, Sundays and No OPD on
top tube (with serum gel separator) Transport
Glucose (FBS) Serum 2 cc blood Holidays Mon-Fri weekends and 6 hours 126.00
immediately
holidays
Stand for 30 minutes Centrifuge with ice
*For STAT patients
and separate serum immediately
anytime of the day
into cryovial

*at least 8 hours Fasting

Monday to Friday (8am-
5pm); No OPD on
Collect in red top (plain), or yellow
Saturdays, Sundays and No OPD on
top tube (with serum gel separator) Transport
Glucose (OGTT) Serum 2 cc blood Holidays Mon-Fri weekends and 6 hours 504.00
immediately
holidays
Stand for 30 minutes Centrifuge with ice
*For STAT patients
and separate serum immediately
anytime of the day
into cryovial

Monday to Friday (8am-

Collect in red top (plain), or yellow
5pm); No OPD on
top tube (with serum gel separator)
Saturdays, Sundays and No OPD on
Transport
Glucose (1 hr. PPBS) Serum 2 cc blood Holidays Mon-Fri weekends and 6 hours 252.00
Stand for 30 minutes Centrifuge immediately
holidays
and separate serum immediately with ice
*For STAT patients
into cryovial
anytime of the day

Monday to Friday (8am-

Collect in red top (plain), or yellow
5pm); No OPD on
top tube (with serum gel separator)
Saturdays, Sundays and No OPD on
Glucose (2 hrs. Transport
Serum 2 cc blood Holidays Mon-Fri weekends and 6 hours 252.00
PPBS) Stand for 30 minutes Centrifuge immediately
holidays
and separate serum immediately with ice
*For STAT patients
into cryovial
anytime of the day

Monday to Friday (8am-

Collect in red top (plain), or yellow No OPD on
Glucose (3 hrs. 5pm); No OPD on Transport
Serum 2 cc blood top tube (with serum gel separator) Mon-Fri weekends and 6 hours 252.00
PPBS) Saturdays, Sundays and immediately
Holidays holidays
Stand for 30 minutes Centrifuge with ice

Page 3 of 64
 AVAILABLE
VOLUME/ TIME OF HANDLING/ CUT OFF DAY
LABORATORY SPECIMEN COLLECTION/ STORAGE RUNNING DAY TAT FEE
QUANTITY COLLECTION TRANSPORT AND TIME
PROCEDURE
*For STAT patients and separate serum immediately
anytime of the day into cryovial

Monday to Friday (8am-

Collect in red top (plain), or yellow
5pm); No OPD on
top tube (with serum gel separator)
Saturdays, Sundays and No OPD on
Glucose (RBS) Serum 2 cc blood Holidays -- Mon-Fri weekends and 6 hours 126.00
Stand for 30 minutes Centrifuge
holidays
and separate serum immediately
*For STAT patients
into cryovial
anytime of the day

Monday to Friday (8am-

Collect in red top (plain), or yellow
5pm); No OPD on
top tube (with serum gel separator)
Saturdays, Sundays and No OPD on
Glucose (1 hr.
Serum 2 cc blood Holidays -- Mon-Fri weekends and 6 hours 126.00
Challenge Test) Stand for 30 minutes Centrifuge
holidays
and separate serum immediately
*For STAT patients
into cryovial
anytime of the day

10-12 hours Fasting

Monday to Friday (8am-
5pm); No OPD on
Collect in red top (plain), or yellow
Saturdays, Sundays and No OPD on
top tube (with serum gel separator) Transport
Cholesterol Serum 2 cc blood Holidays Mon-Fri weekends and 6 hours 210.00
immediately
holidays
Stand for 30 minutes Centrifuge with ice
*For STAT patients
and separate serum immediately
anytime of the day
into cryovial

10-12 hours Fasting

Monday to Friday (8am-
5pm); No OPD on
Collect in red top (plain), or yellow
Saturdays, Sundays and No OPD on
top tube (with serum gel separator) Transport
HDL – Cholesterol Serum 2 cc blood Holidays Mon-Fri weekends and 6 hours 420.00
immediately
holidays
Stand for 30 minutes Centrifuge with ice
*For STAT patients
and separate serum immediately
anytime of the day
into cryovial

Monday to Friday (8am- 10-12 hours Fasting No OPD on

Transport
LDL – Cholesterol Serum 2 cc blood 5pm); No OPD on Mon-Fri weekends and 6 hours 511.00
Saturdays, Sundays and Collect in red top (plain), or yellow immediately
holidays
Holidays top tube (with serum gel separator) with ice

Page 4 of 64
 AVAILABLE
VOLUME/ TIME OF HANDLING/ CUT OFF DAY
LABORATORY SPECIMEN COLLECTION/ STORAGE RUNNING DAY TAT FEE
QUANTITY COLLECTION TRANSPORT AND TIME
PROCEDURE

*For STAT patients Stand for 30 minutes Centrifuge

anytime of the day and separate serum immediately
into cryovial

10-12 hours Fasting

Monday to Friday (8am-
5pm); No OPD on
Collect in red top (plain), or yellow
Saturdays, Sundays and No OPD on
top tube (with serum gel separator) Transport
Triglycerides Serum 2 cc blood Holidays Mon-Fri weekends and 6 hours 252.00
immediately
holidays
Stand for 30 minutes Centrifuge with ice
*For STAT patients
and separate serum immediately
anytime of the day
into cryovial

Monday to Friday (8am-

Collect in red top (plain), or yellow
5pm); No OPD on
top tube (with serum gel separator)
Saturdays, Sundays and No OPD on
Transport
Albumin Serum 2 cc blood Holidays Mon-Fri weekends and 6 hours 196.00
Stand for 30 minutes Centrifuge immediately
holidays
and separate serum immediately with ice
*For STAT patients
into cryovial
anytime of the day

Monday to Friday (8am-

Collect in red top (plain), or yellow
5pm); No OPD on
top tube (with serum gel separator)
Saturdays, Sundays and No OPD on
Transport
Alkaline Phosphate Serum 2 cc blood Holidays Mon-Fri weekends and 6 hours 196.00
Stand for 30 minutes Centrifuge immediately
holidays
and separate serum immediately with ice
*For STAT patients
into cryovial
anytime of the day

Collect in red top (plain), or yellow

Monday to Friday (8am- top tube (with serum gel separator)
5pm); No OPD on
Saturdays, Sundays and Stand for 30 minutes Centrifuge No OPD on
Transport
Bilirubin, Direct Serum 2 cc blood Holidays and separate serum immediately Mon-Fri weekends and 6 hours 196.00
immediately
into cryovial holidays
with ice
*For STAT patients
anytime of the day Note: Avoid direct sunlight; cover
tube with carbon paper

Page 5 of 64
 AVAILABLE
VOLUME/ TIME OF HANDLING/ CUT OFF DAY
LABORATORY SPECIMEN COLLECTION/ STORAGE RUNNING DAY TAT FEE
QUANTITY COLLECTION TRANSPORT AND TIME
PROCEDURE
Collect in red top (plain), or yellow
Monday to Friday (8am- top tube (with serum gel separator)
5pm); No OPD on
Saturdays, Sundays and Stand for 30 minutes Centrifuge No OPD on
Transport
Bilirubin, Total Serum 2 cc blood Holidays and separate serum immediately Mon-Fri weekends and 6 hours 392.00
immediately
into cryovial holidays
with ice
*For STAT patients
anytime of the day Note: Avoid direct sunlight; cover
tube with carbon paper

Collect in red top (plain), or yellow

Monday to Friday (8am- top tube (with serum gel separator)
5pm); No OPD on
Saturdays, Sundays and Stand for 30 minutes Centrifuge No OPD on
Bilirubin, (Direct, Transport
Serum 2 cc blood Holidays and separate serum immediately Mon-Fri weekends and 6 hours 588.00
Indirect & Total) immediately
into cryovial holidays
with ice
*For STAT patients
anytime of the day Note: Avoid direct sunlight; cover
tube with carbon paper

Monday to Friday (8am-

Collect in red top (plain), or yellow
5pm); No OPD on
top tube (with serum gel separator)
Saturdays, Sundays and No OPD on
Transport
SGOT Serum 2 cc blood Holidays Mon-Fri weekends and 6 hours 168.00
Stand for 30 minutes Centrifuge immediately
holidays
and separate serum immediately with ice
*For STAT patients
into cryovial
anytime of the day

Monday to Friday (8am-

Collect in red top (plain), or yellow
5pm); No OPD on
top tube (with serum gel separator)
Saturdays, Sundays and No OPD on
Transport
SGPT Serum 2 cc blood Holidays Mon-Fri weekends and 6 hours 168.00
Stand for 30 minutes Centrifuge immediately
holidays
and separate serum immediately with ice
*For STAT patients
into cryovial
anytime of the day

Collect in red top (plain), or yellow

Monday to Friday (8am- top tube (with serum gel separator)
No OPD on
5pm); No OPD on Transport
Total Protein Serum 2 cc blood Mon-Fri weekends and 6 hours 196.00
Saturdays, Sundays and Stand for 30 minutes Centrifuge immediately
Holidays holidays
and separate serum immediately with ice
into cryovial

Page 6 of 64
 AVAILABLE
VOLUME/ TIME OF HANDLING/ CUT OFF DAY
LABORATORY SPECIMEN COLLECTION/ STORAGE RUNNING DAY TAT FEE
QUANTITY COLLECTION TRANSPORT AND TIME
PROCEDURE
*For STAT patients
anytime of the day

Monday to Friday (8am-

Collect in red top (plain), or yellow
5pm); No OPD on
top tube (with serum gel separator)
Saturdays, Sundays and No OPD on
TPA/G (Total Protein Transport
Serum 2 cc blood Holidays Mon-Fri weekends and 6 hours 588.00
A/G) Stand for 30 minutes Centrifuge immediately
holidays
and separate serum immediately with ice
*For STAT patients
into cryovial
anytime of the day

Monday to Friday (8am-

Collect in red top (plain), or yellow
5pm); No OPD on
top tube (with serum gel separator)
Saturdays, Sundays and No OPD on
Transport
BUN Serum 2 cc blood Holidays Mon-Fri weekends and 6 hours 168.00
Stand for 30 minutes Centrifuge immediately
holidays
and separate serum immediately with ice
*For STAT patients
into cryovial
anytime of the day

Monday to Friday (8am-

Collect in red top (plain), or yellow
5pm); No OPD on
top tube (with serum gel separator)
Saturdays, Sundays and No OPD on
Transport
Creatinine Serum 2 cc blood Holidays Mon-Fri weekends and 6 hours 168.00
Stand for 30 minutes Centrifuge immediately
holidays
and separate serum immediately with ice
*For STAT patients
into cryovial
anytime of the day

Monday to Friday (8am-

Collect in red top (plain), or yellow
5pm); No OPD on
top tube (with serum gel separator)
Saturdays, Sundays and
Transport
Amylase Serum 2 cc blood Holidays Daily -- 6 hours 350.00
Stand for 30 minutes Centrifuge immediately
and separate serum immediately with ice
*For STAT patients
into cryovial
anytime of the day

Collect in red top (plain), or yellow

Monday to Friday (8am- top tube (with serum gel separator)
5pm); No OPD on Transport
CPK / CK Serum 2 cc blood Saturdays, Sundays and Daily -- 6 hours 430.00
Stand for 30 minutes Centrifuge immediately
Holidays and separate serum immediately with ice
into cryovial

Page 7 of 64
 AVAILABLE
VOLUME/ TIME OF HANDLING/ CUT OFF DAY
LABORATORY SPECIMEN COLLECTION/ STORAGE RUNNING DAY TAT FEE
QUANTITY COLLECTION TRANSPORT AND TIME
PROCEDURE
*For STAT patients
anytime of the day

Monday to Friday (8am-

Collect in red top (plain), or yellow
5pm); No OPD on
top tube (with serum gel separator)
Saturdays, Sundays and
Transport
LDH Serum 2 cc blood Holidays Daily -- 6 hours 340.00
Stand for 30 minutes Centrifuge immediately
and separate serum immediately with ice
*For STAT patients
into cryovial
anytime of the day

6-8 hours Fasting

Monday to Friday (8am-
5pm); No OPD on
Collect in red top (plain), or yellow
Saturdays, Sundays and
top tube (with serum gel separator) Transport
Uric Acid Serum 2 cc blood Holidays Daily -- 6 hours 210.00
immediately
Stand for 30 minutes Centrifuge with ice
*For STAT patients
and separate serum immediately
anytime of the day
into cryovial

Collect in sterile bottle

Monday to Friday (8am-

5pm); No OPD on
Saturdays, Sundays and
Transport
CSF Glucose CSF 1 cc Holidays Daily -- 6 hours 252.00
immediately
with ice
*For STAT patients
anytime of the day

Monday to Friday (8am-

5pm); No OPD on
Saturdays, Sundays and
Transport
CSF Protein CSF 1 cc Holidays Collect in sterile bottle Daily -- 6 hours 252.00
immediately
with ice
*For STAT patients
anytime of the day

Page 8 of 64
 AVAILABLE
VOLUME/ TIME OF HANDLING/ CUT OFF DAY
LABORATORY SPECIMEN COLLECTION/ STORAGE RUNNING DAY TAT FEE
QUANTITY COLLECTION TRANSPORT AND TIME
PROCEDURE
Monday to Friday (8am-
Collect in red top (plain), or yellow
5pm); No OPD on
top tube (with serum gel separator)
Saturdays, Sundays and
Transport
Sodium (Na) Serum 2 cc blood Holidays Daily -- 2 hours 322.00
Stand for 30 minutes Centrifuge immediately
and separate serum immediately with ice
*For STAT patients
into cryovial
anytime of the day

Monday to Friday (8am-

Collect in red top (plain), or yellow
5pm); No OPD on
top tube (with serum gel separator)
Saturdays, Sundays and
Transport
Potassium (K) Serum 2 cc blood Holidays Daily -- 2 hours 322.00
Stand for 30 minutes Centrifuge immediately
and separate serum immediately with ice
*For STAT patients
into cryovial
anytime of the day

Monday to Friday (8am-

Collect in red top (plain), or yellow
5pm); No OPD on
top tube (with serum gel separator)
Saturdays, Sundays and
Transport
Chloride (Cl) Serum 2 cc blood Holidays Daily -- 2 hours 218.00
Stand for 30 minutes Centrifuge immediately
and separate serum immediately with ice
*For STAT patients
into cryovial
anytime of the day

Monday to Friday (8am-

Collect in red top (plain), or yellow
5pm); No OPD on
top tube (with serum gel separator)
Saturdays, Sundays and
Transport
Magnesium (Mg) Serum 2 cc blood Holidays Daily -- 2 hours 337.00
Stand for 30 minutes Centrifuge immediately
and separate serum immediately with ice
*For STAT patients
into cryovial
anytime of the day

Monday to Friday (8am-

Collect in red top (plain), or yellow
5pm); No OPD on
top tube (with serum gel separator)
Saturdays, Sundays and
Transport
Calcium (Ca) Serum 2 cc blood Holidays Daily -- 2 hours 300.00
Stand for 30 minutes Centrifuge immediately
and separate serum immediately with ice
*For STAT patients
into cryovial
anytime of the day

Page 9 of 64
 AVAILABLE
VOLUME/ TIME OF HANDLING/ CUT OFF DAY
LABORATORY SPECIMEN COLLECTION/ STORAGE RUNNING DAY TAT FEE
QUANTITY COLLECTION TRANSPORT AND TIME
PROCEDURE
Monday to Friday (8am-
Collect in red top (plain), or yellow
5pm); No OPD on
top tube (with serum gel separator)
Saturdays, Sundays and
Inorganic Transport
Serum 2 cc blood Holidays Daily -- 2 hours 168.00
Phosphorous Stand for 30 minutes Centrifuge immediately
and separate serum immediately with ice
*For STAT patients
into cryovial
anytime of the day

Page 10 of 64
ROUTINE LABORATORY TESTS: CLINICAL MICROSCOPY
AVAILABLE
VOLUME/ TIME OF HANDLING/ RUNNING CUT OFF DAY
LABORATORY SPECIMEN COLLECTION/ STORAGE TAT FEE
QUANTITY COLLECTION TRANSPORT DAY AND TIME
PROCEDURE
300.00
Cell Count /
Transport
Differential Count CSF at least 8 ml anytime Collect in sterile bottle Daily -- 2 hours
immediately
of Body Fluids
with ice

140.00
early morning/anytime Transport
Urinalysis Urine 15-30 ml Collect in sterile bottle Daily -- 2 hours
of the day immediately
with ice

RBC Morphology Urine 15-30 ml early morning/anytime Collect in sterile bottle Daily -- 2 hours 140.00
of the day Transport
immediately
with ice

Direct fecal Smear Stool Approximately anytime Collect in a clean stool container Room Mon-Fri Monday-Friday 1 hour 210.00
Microscopy adult thumb temperature 25 (8AM-5PM)
size to 37 degree *After 5PM,
Celsius

Watery and soft

specimen must
reach the
laboratory w/in
1hr from the
time of passage

Fecal Occult Blood Stool Approximately anytime Collect in a clean stool container Room Mon-Fri Monday-Friday 30 minutes 140.00
Test adult thumb temperature 25 (8AM-5PM)
size to 37 degree *After 5PM,
Celsius

Page 11 of 64
 AVAILABLE
VOLUME/ TIME OF HANDLING/ RUNNING CUT OFF DAY
LABORATORY SPECIMEN COLLECTION/ STORAGE TAT FEE
QUANTITY COLLECTION TRANSPORT DAY AND TIME
PROCEDURE

Microscopy for Peri-anal Duplicate (2) Collection before -- -- Mon-Fri Monday-Friday 1 hour 112.00
Enterobius vermicularis swab taking a bath (8AM-5PM)
*After 5PM, please
submit specimen
and request through
Clinical Laboratory

Malaria blood film Blood 5-10 drops (duplicate) anytime Finger prick preferred Room temperature 25 Mon-Fri Monday-Friday 1 hour 210.00
microscopy (thick & Avoid exposure of smear to heat to 37 degree Celsius (8AM-5PM)
thin film) Protect smear from dust and insects *After 5PM, please
submit specimen
and request through
Clinical Laboratory

Page 12 of 64
ROUTINE LABORATORY TESTS: CLINICAL MICROBIOLOGY (BACTERIOLOGIC TESTS)
AVAILABLE CUT OFF
VOLUME/ TIME OF HANDLING/ RUNNING
LABORATORY SPECIMEN COLLECTION/ STORAGE DAY AND TAT FEE
QUANTITY COLLECTION TRANSPORT DAY
PROCEDURE TIME
Transport as soon as
possible at room
Gram stain, Preliminary
1-2 ml (If temperature (RT);
Culture and result:
commercial Acute stage of illness Aseptically collect blood and ≤ 2 hours RT;
Susceptibility after 48
Blood bottle: follow prior to antimicrobial inoculate in Blood Culture Broth (BCB) < 24 hours, RT Daily - 1,320.00
Test hours
manufacturer’s therapy (BCB) and mix by swirling. (If not transported
Final result
instructions.) after 2 hours, incubate
(Aerobic Culture) : 8 days
36 + 1oC prior to
transport)

Transport as soon as -
possible.
≤15 min., RT;
< 24 hours, RT;
(if BAP and CA are
submitted, if possible, Preliminary
Gram stain, include the remaining result after
Culture and
Acute stage of illness CSF, RT) 24 hours.
Susceptibility Collect aseptically in a sterile
CSF ≥ 1 ml prior to antimicrobial (If transport may be Daily Final result 1,320.00
Test screw-cap container.
therapy delayed, incubate the after 3
specimen at 36+ 1oC days if no
(Aerobic Culture)
and/or may directly growth.
inoculate onto Blood
Agar (BAP) and
Chocolate Agar (CA)
and incubate at 36+
1oC prior to transport

Collection is performed by a -
clinician. Transport as soon as Preliminary
Gram stain, Sterile body Fluids:
1. Disinfect underlying skin with possible. result after
Culture and pleural, synovial,
Acute stage of illness iodine. ≤15 min., RT; 24 hours.
Susceptibility peritoneal, minimum of 1- 2. Collect specimen via > 1 hour, on ice packs
prior to antimicrobial Daily Final result 1,320.00
Test pericardial, bile, 2 ml percutaneous needle aspiration
therapy after 3
joints, ascites,
or surgery. Storage: days if no
(Aerobic Culture) abdominal 3. Inoculate approx. 1-2 mL of refrigerate prior to growth.
specimen into the Blood Culture transport and include
Broth and mix by swirling.

Page 13 of 64
 AVAILABLE CUT OFF
VOLUME/ TIME OF HANDLING/ RUNNING
LABORATORY SPECIMEN COLLECTION/ STORAGE DAY AND TAT FEE
QUANTITY COLLECTION TRANSPORT DAY
PROCEDURE TIME
**If further testing is the remaining
anticipated, the rest of the specimen)
sample may be saved

Gram stain, -
Transport as soon as
Culture and Sputum Acute stage of illness Rinse the mouth with water and possible. Final result
Susceptibility
(Expectorated or 3-5 ml prior to antimicrobial bring up a deep cough. Collect ≤2 hours, RT; Daily after 1,320.00
Test ≤ 24 hours, place on
induced) therapy in a sterile screw-cap container. 2-5 days
ice pack
(Aerobic Culture)

Gram stain, Endotracheal/ -

Transport as soon as
Culture and Nasotracheal Collect washing or aspirate in
Acute stage of illness possible. Final result
Susceptibility Aspirate; mucus trap;
≥ 1 ml prior to antimicrobial ≤2 hours, RT; Daily after 1,320.00
Test Bronchoalveolar Place brush in 1 ml sterile NSS
therapy ≤ 24 hours, place on 2-5 days
lavage, brush or in a sterile container
(Aerobic Culture) ice packs
wash

Swab the posterior pharynx, -

Unpreserved:
tonsils and other inflamed
Transport as soon as
Culture and areas.
possible; ≤ 2 hours,
Susceptibility Acute stage of illness Final result
Throat Swab RT;
Test 2 swabs prior to antimicrobial Unpreserved, place the swabs Daily after 1,320.00
(not diphtheria)
therapy in sterile tube 2-5 days
Preserved:
(Aerobic Culture)
≤ 24 hours, Amies TM,
Preserved, place in
RT
Amies Transport Medium (TM)

Transport as soon as -
First morning voided urine is possible; < 2 hours,
preferred. RT; ≤ 24 hours, on ice
Culture and packs
Susceptibility Acute stage of illness Final result
Urine -Cleanse the genital area with
Test 2-5 ml prior to antimicrobial Daily after 1,320.00
a. fresh voided soap and water (Note: the container
therapy 2-5 days
must be wrapped with
(Aerobic Culture)
-then collect midstream portion a tape or sealing film
in a sterile leak-proof container. when transported with
ice packs)

Page 14 of 64
 AVAILABLE CUT OFF
VOLUME/ TIME OF HANDLING/ RUNNING
LABORATORY SPECIMEN COLLECTION/ STORAGE DAY AND TAT FEE
QUANTITY COLLECTION TRANSPORT DAY
PROCEDURE TIME
1. Straight catheter: -

-Cleansing the urethral opening

with soap and water.

-Allow to pass �15 mL of urine.

Collect in a sterile leak-proof Transport as soon as
container. possible;
2-5 ml
Culture and b. Urine 2. Indwelling catheter: Collect Transport as soon as
(straight
Susceptibility catheterized: Acute stage of illness only from patient w/c are possible; Final result
catheter)
Test 1. Straight catheter prior to antimicrobial symptomatic. -Disinfect the < 2 hours, RT; Daily after 1,320.00
5-10 ml
2. Indwelling therapy catheter collection port with 2-5 days
(indwelling
(Aerobic Culture) catheter 70% alcohol. ≥ 2 hours, on ice packs
catheter)
(see note above when
-Clamp catheter below port transported with ice
allow to collect in tubing for 20- packs)
30 min.

-Use needle and syringe to

aseptically collect 5-10 ml of
urine aseptically and transfer in
a sterile leak-proof container.

Aspirate specimen with sterile -

needle into a sterile leak-proof
Transport as soon as
Culture and container.
possible.
Susceptibility Acute stage of illness Final result
> 2 hours, on ice packs
Test Exudate 0.5-1 ml prior to antimicrobial s Unpreserved: place the swabs Daily after 1,320.00
(see note above when
therapy in sterile tube 2-5 days
transported with ice
(Aerobic Culture)
packs)
s Preserved: place in
Amies Transport Medium (TM)

Collect sufficient materials from -

Unpreserved:
affected area. Place swabs in
Culture and Transport as soon as
sterile tubes. Collect another
Susceptibility possible; > 2 hours, Final result
2 swabs & Preferably prior to swab for smear on a clean glass
Test Surface wound place on ice packs. Daily after 1,320.00
smear antimicrobial therapy slides. Label clearly
(see note above when 2-5 days
(Aerobic Culture) transported with ice
Unpreserved: place the swabs
packs)
in sterile tube

Page 15 of 64
 AVAILABLE CUT OFF
VOLUME/ TIME OF HANDLING/ RUNNING
LABORATORY SPECIMEN COLLECTION/ STORAGE DAY AND TAT FEE
QUANTITY COLLECTION TRANSPORT DAY
PROCEDURE TIME
Preserved: place in Amies Preserved: ≤ 24 hours,
Transport Medium ™ Amies TM, RT

Open: remove the surface -

exudate by wiping with alcohol
pad or with sterile saline.
Unpreserved:
Aspirate if possible or pass the
Transport as soon as
swab deep into the lesion to
possible; > 2 hours,
Culture and firmly sample the fresh border.
place on ice packs.
Susceptibility Final result
0.5-1 ml or 2 Preferably prior to (see note above when
Test Abscess Closed: disinfect the site and Daily after 1,320.00
swabs antimicrobial therapy transported with ice
aspirate with needle and syringe 2-5 days
packs)
(Aerobic Culture) into a sterile container.
Preserved:≤ 24 hours,
Swabs: sterile container
Amies TM, RT
Aspirates: transfer in a sterile
container

Unpreserved:Transport -
as soon as possible;
Collected during surgery or
Preserved:
cutaneous biopsy procedure:-
≤ 24 hours., RT
place in sterile leak-proof
Culture and >24 hours, on ice
container.
Susceptibility Tissue/Biopsy Minimum packs (see note Final result
Preferably prior to
Test material (see diameter of 1 above) Daily after 1,320.00
antimicrobial therapy Unpreserved: Dry sterile
closed abscess) cm 2-5 days
container
(Aerobic Culture) Storage:
≤ 24 hours., RT;
Preserved:Immerse specimen in
>24 hours, refrigerate
NSS
(4-8oC) prior to
transport suspend in
0.5-1 ml in sterile NSS

Culture and Endocervical, Female: collect specimen from Unpreserved: Swabs: -

Susceptibility vaginal /male Acute stage of illness endocervical canal with 2 Transport immediately
2 swabs and 1- swabs.
Test urethral/penile prior to antimicrobial Daily 3-7 days 1,320.00
2 smears Preserved:
discharge/anorectal therapy
(Aerobic Culture) swab Male: collect discharge or insert CA and
the small swab into the urethra. MTM/BAP(optional):

Page 16 of 64
 AVAILABLE CUT OFF
VOLUME/ TIME OF HANDLING/ RUNNING
LABORATORY SPECIMEN COLLECTION/ STORAGE DAY AND TAT FEE
QUANTITY COLLECTION TRANSPORT DAY
PROCEDURE TIME
≤ 24 hours in candle
Anorectal swab-Insert a sterile jar, RT;
swab approx. 2.5 cm into anal Amies TM: < 4 hours,
canal and allow swab to remain RT
10-30 sec for absorption.

Unpreserved: sterile container

Preserved: Place one swab in

Amies Transport Medium; and
the other swab to make a smear
for gram stain. (air dry the
smear then cover with another
clean slide and wrap with a
clean paper)

Unpreserved: ≤ 2 -
Unpreserved: Collect into a hours, RT; < 6 hours,
Culture and Formed stool: clean wide-mouth leak-proof Transport on ice packs
Susceptibility Pea or Thumb Acute stage of illness container. (must be processed
Test Stool size prior to antimicrobial within 6 hours after Daily 3-5 days 1,320.00
therapy Preserved: Swab the fresh stool collection)
(Aerobic Culture) Liquid: 2-5 ml and place in Cary Blair Transport
Medium Preserved: ≤ 7 days in
Cary Blair TM, RT

Moisten the swab with sterile -

NSS before collection and insert
the swab into the rectum 1-1.5
1-2 swabs Unpreserved:
Culture and inches and rotate the swab
Note: Transport as soon as
Susceptibility Rectal swab Acute stage of illness gently.
(commercial possible
Test (alternative to prior to antimicrobial Daily 3-5 days 1,320.00
Transport
stool) therapy Unpreserved: Sterile tube
Medium has Preserved:≤ 7 days in
(Aerobic Culture)
only 1 swab) Cary Blair TM, RT
Preserved: Place in a Cary-Blair
Transport Medium (TM)
available at Clinical Laboratory

Pulmonary 3-5 ml Refrigerate if cannot -

Spot or early morning Collect 2 specimens in a sterile 3 working
AFB Smear (Sputum, BAL, /container x 2 be transported Mon-Fri 420.00
specimen preferred transparent, 50 ml-capacity days
NPA) specimen immediately.
screw capped container. To
Transport in ice

Page 17 of 64
 AVAILABLE CUT OFF
VOLUME/ TIME OF HANDLING/ RUNNING
LABORATORY SPECIMEN COLLECTION/ STORAGE DAY AND TAT FEE
QUANTITY COLLECTION TRANSPORT DAY
PROCEDURE TIME
forward to the laboratory as following IATA Two (2)
FRESH as possible procedure. specimens
per test

Refrigerate if cannot - 210.00

be transported
CSF keep at room temperature
immediately. One (1)
or in an incubator(37 degrees
Transport in ice specimen
Extrapulmonary 3-5 ml Celsius)until ready for transport
following IATA 3 working per test
AFB Smear (CSF, tissue /container x 2 -- to the laboratory; Gastric Mon-Fri
procedure. CSF -to days
exudates, skin etc.) specimen aspirate should be delivered No charge
send the specimen
promptly to the laboratory for DOTS
IMMEDIATELY in
within 4 hours. and PMDT
transport box
WITHOUT ICE. patients

Transport -
immediately, RT;
≤ 1 hour, RT;
Rapid Plasma
Transfer separated serum into > 1 hour, transport on
Reagin (RPR) 3 working
(CSF, urine, serum) 1 – 2 ml upon request sterile 2 ml screw-capped vial or ice packs Mon-Wed-Fri 500.00
day
properly sealed red top tube.
QUALITATIVE
Storage:
≥ 1 hour, freeze prior
to transport

Transport -
immediately, RT;
≤ 1 hour, RT;
Rapid Plasma
Transfer separated serum into > 1 hour, transport on
Reagin (RPR) 3 working
(CSF, urine, serum) 1 – 2 ml upon request sterile 2 ml screw-capped vial or ice packs Mon-Wed-Fri 2,500.00
day
properly sealed red top tube.
QUANTITATIVE
Storage:
≥ 1 hour, freeze prior
to transport

Treponema Transport -
Pallidum Particle immediately, RT;
Transfer separated serum into
Agglutination ≤ 1 hour, RT; 3 working
Serum 1 – 2 ml upon request sterile 2 ml screw-capped vial or > 1 hour, transport on Mon-Wed-Fri 1,155.00
(TPPA) day
properly sealed red top tube. ice packs
QUALITATIVE
Storage:

Page 18 of 64
 AVAILABLE CUT OFF
VOLUME/ TIME OF HANDLING/ RUNNING
LABORATORY SPECIMEN COLLECTION/ STORAGE DAY AND TAT FEE
QUANTITY COLLECTION TRANSPORT DAY
PROCEDURE TIME
≥ 1 hour, freeze prior
to transport

Transport -
Treponema immediately, RT;
Pallidum Particle ≤ 1 hour, RT;
Agglutination Transfer separated serum into > 1 hour, transport on
(TPPA) 3 working
Serum 1 – 2 ml upon request sterile 2 ml screw-capped vial or ice packs Mon-Wed-Fri 2,500.00
day
properly sealed red top tube.
QUANTITATIVE
Storage:
≥ 1 hour, freeze prior
to transport

ROUTINE LABORATORY TESTS: CLINICAL MICROBIOLOGY (VIROLOGIC TESTS)

AVAILABLE CUT OFF
VOLUME/ TIME OF HANDLING/ RUNNING
LABORATORY SPECIMEN COLLECTION/ STORAGE DAY AND TAT FEE
QUANTITY COLLECTION TRANSPORT DAY
PROCEDURE TIME
Collect in red /yellow top tube
Hepatitis A Virus
and aliquot sample into (see collection / Monday (4:00 6 working
Serology (Anti- Serum 1 ml anytime Tuesday 500.00
cryotube. Transport at cold storage procedures) PM) days
HAV IgM)
chain / in ice within 72 hours.

Collect in red /yellow top tube

Hepatitis B Virus and aliquot sample into (see collection / Wednesday 6 working
Serum 1 ml anytime Thursday 252.00
Serology (HBsAg) cryotube. Transport at cold storage procedures) (4:00PM) days
chain / in ice within 72 hours.

Collect in red /yellow top tube

Hepatitis B Virus
and aliquot sample into (see collection / Wednesday 6 working
Serology (Anti- Serum 1 ml anytime Thursday 420.00
cryotube. Transport at cold storage procedures) (4:00 PM) days
HBsAg)
chain / in ice within 72 hours.

Page 19 of 64
 AVAILABLE CUT OFF
VOLUME/ TIME OF HANDLING/ RUNNING
LABORATORY SPECIMEN COLLECTION/ STORAGE DAY AND TAT FEE
QUANTITY COLLECTION TRANSPORT DAY
PROCEDURE TIME
Collect in red /yellow top tube
Hepatitis B Virus and aliquot sample into (see collection / Tuesday (4:00 6 working
Serum 1 ml anytime Wednesday 300.00
Serology (HBeAg) cryotube. Transport at cold storage procedures) PM) days
chain / in ice within 72 hours.

Collect in red /yellow top tube

Hepatitis B Virus
and aliquot sample into (see collection / Tuesday (4:00 6 working
Serology (Anti- Serum 1 ml anytime Wednesday 300.00
cryotube. Transport at cold storage procedures) PM) days
HBeAg)
chain / in ice within 72 hours.

Collect in red /yellow top tube

Hepatitis B Virus
and aliquot sample into (see collection / Friday (4:00 6 working
Serology (Anti- Serum 1 ml anytime Monday 300.00
cryotube. Transport at cold storage procedures) PM) days
HBc)
chain / in ice within 72 hours.

Collect in red /yellow top tube

Hepatitis C Virus
and aliquot sample into (see collection / Monday (4:00 6 working
Serology (Anti- Serum 1 ml anytime Tuesday 600.00
cryotube. Transport at cold storage procedures) PM) days
HCV)
chain / in ice within 72 hours.

Dengue Virus
Collect in red plain tube. No
NS1-Ag (Rapid Serum 2 cc blood Mon-Fri -- Daily -- 2 hours 700.00
inversion.
Test)

Dengue Virus
Collect in red plain tube. No
IgM & IgG (Rapid Serum 2 cc blood Mon-Fri -- Daily -- 2 hours 700.00
inversion.
Test)

To be collected by RITM Clinical

HIV 1 & 3 5 cc x 2 No Charge
Laboratory Staff, after
-- yellow top Mon-Fri (8-3 PM) -- Mon-Fri 3PM 3 hours for OHAT
undergoing pre-counseling
HAT Screening SST tube Patients
from RITM Counsellor.

Page 20 of 64
ROUTINE LABORATORY TESTS: ANATOMIC PATHOLOGY
AVAILABLE CUT OFF
VOLUME/ TIME OF HANDLING/ RUNNING
LABORATORY SPECIMEN COLLECTION/ STORAGE DAY AND TAT FEE
QUANTITY COLLECTION TRANSPORT DAY
PROCEDURE TIME
210.00
(small)

360.00
(medium)

560.00
(large)
Small, less 7 -10
than 3 cm Tue-Thu working 640.00
Upon biopsy or autopsy soak Tissue specimen could (radical)
Medium 3-5 days
Hematoxylin- sample in Buffered Neutral be left in BNF
Tissue cm -- (STAT: a day 4PM
Eosin Stain Formalin (BNF) at least 1:15 indefinitely at room STAT
Large 5-7 cm after specimen STAT: 3-5
ratio temperature 350.00
Radical, more receipt) working (small)
than 7 cm days
510.00
(medium)

780.00
(large)

1,190.00
(radical)
2-3 days after
Modified Acid Upon biopsy or autopsy soak Tissue specimen could receipt 350.00
3-7
Fast (MAF)/ sample in Buffered Neutral be left in BNF
Tissue n/a -- 4PM working
Modified Gram's Formalin (BNF) at least 1:15 indefinitely at room (STAT: a day STAT
days
Stain (MGS) ratio temperature after specimen 530.00
receipt)
2-3 days after
Upon biopsy or autopsy soak Tissue specimen could receipt 350.00
3-7
Periodic Acid sample in Buffered Neutral be left in BNF
Tissue n/a -- 4PM working
Schiff (PAS) Formalin (BNF) at least 1:15 indefinitely at room (STAT: a day STAT
days
ratio temperature after specimen 530.00
receipt)

Page 21 of 64
 AVAILABLE CUT OFF
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LABORATORY SPECIMEN COLLECTION/ STORAGE DAY AND TAT FEE
QUANTITY COLLECTION TRANSPORT DAY
PROCEDURE TIME

Next day after 7-10

Recut of Tissue
Tissue sections -- -- -- -- receipt of -- working 100.00
for H & E
request days

Recut for Next day after 7-10

Unstained slide Tissue sections -- -- -- -- receipt of -- working 100.00
for send out request days

Pull-out of Tissue Requires approved letter of

Tissue Block -- -- -- -- -- -- 280.00
Block request after validation

Upon collection, send

immediately to the
7-10
Cell block with laboratory or store in Same day
Body fluids 3 - 5 ml -- -- -- working 600.0
Cytology the ref. if not processing
days
processed
immediately
Upon collection, send
immediately to the
7-10
Cell block with laboratory or store in Same day
Pleural fluids 100 - 300 ml -- -- 12NN working 600.00
Cytology the ref. if not processing
days
processed
immediately

Final Autopsy Full/partial tissue

-- -- -- -- -- -- 1 month 5,000.00
report organs

Referrals from 42.00

3-5
RITM One (1)
Tissue biopsy -- -- -- -- -- -- working
Pathologists: H & tissue
days
E cassette
Referrals from
RITM 3-5 16.00
Body fluids/
Pathologists: -- -- -- -- -- -- working One (1)
vaginal smear
PAPs /Tzank days Slide
smear/ Cytology

Page 22 of 64
 AVAILABLE CUT OFF
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LABORATORY SPECIMEN COLLECTION/ STORAGE DAY AND TAT FEE
QUANTITY COLLECTION TRANSPORT DAY
PROCEDURE TIME
2-3 days after
3-7
Upon biopsy or autopsy soak Tissue specimen could receipt 350.00
working
Mucicarmine sample in Buffered Neutral be left in BNF
Tissue n/a -- 4PM days
Stain Formalin (BNF) at least 1:15 indefinitely at room (STAT: a day STAT
ratio temperature after specimen 490.00
STAT: ??
receipt)
3-7
2-3 days after
working
Upon biopsy or autopsy soak Tissue specimen could receipt 420.00
Gomorri's days
sample in Buffered Neutral be left in BNF
Ammoniacal Tissue n/a -- 4PM
Formalin (BNF) at least 1:15 indefinitely at room (STAT: a day STAT
Silver Stain (GAS) STAT: 3
ratio temperature after specimen 600.00
working
receipt)
days
Soak the smear for at
Fresh smears are made on pre-
Smears on glass least 15 minutes and 200.00
labeled glass slides in a circular
slide in 95% then air dry and send 7 working
Tzanck Smear n/a -- motion, allow to dry at the edges Mon-Fri 12NN
ethanol for at least to the lab. In slide days STAT
and immerse immediately in
15 minutes mailers with proper 300.00
95% ethanol
label

Page 23 of 64
SPECIAL LABORATORY TESTS: ELECTRON MICROSCOPY
AVAILABLE CUT OFF
VOLUME/ TIME OF HANDLING/ RUNNING
LABORATORY SPECIMEN COLLECTION/ STORAGE DAY AND TAT FEE
QUANTITY COLLECTION TRANSPORT DAY
PROCEDURE TIME

Transport in ice ASAP.

5,000.00
Package A/TEM If not possible wash
3mm x 3mm in Upon biopsy or autopsy soak (Non-
tissue with 2 changes
1-2 ml 2.5% sample in cold (4oC) 2.5% clinical
Complete tissue Tissue -- of phosphate buffer & Monday -- 2 weeks
glutaraldehyde glutaraldehyde at 1:10 ratio for sample)
processing with store in refrigerator in
solution 2.5 hours
micrograph phosphate buffer at 10,000.00
1:10 ratio (Clinical
sample)

Transport in ice ASAP.

5,000.00
Package A/TEM If not possible wash
Upon biopsy or autopsy soak (Non-
tissue with 2 changes
Fixative of 1:10 sample in cold (4oC) 2.5% clinical
Complete tissue Body fluids -- of phosphate buffer & Monday -- 2 weeks
ratio glutaraldehyde at 1:10 ratio for sample)
processing with store in refrigerator in
2.5 hours
micrograph phosphate buffer at 10,000.00
1:10 ratio (Clinical
sample)

Transport in ice ASAP. 4,200.00

Package B/TEM If not possible wash
3mm x 3mm in Upon biopsy or autopsy soak Non-
tissue with 2 changes
1-2 ml 2.5% sample in cold (4oC) 2.5% clinical
Specimen Tissue -- of phosphate buffer & Monday -- 1 week
glutaraldehyde glutaraldehyde at 1:10 ratio for sample
processing with store in refrigerator in
solution 2.5 hours
viewing only phosphate buffer at
1:10 ratio

Transport in ice ASAP. 4,200.00

Package B/TEM If not possible wash
Upon biopsy or autopsy soak Non-
tissue with 2 changes
Fixative of 1:10 sample in cold (4oC) 2.5% clinical
Specimen Body fluids -- of phosphate buffer & Monday -- 1 week
ratio glutaraldehyde at 1:10 ratio for sample
processing with store in refrigerator in
2.5 hours
viewing only phosphate buffer at
1:10 ratio

Page 24 of 64
 AVAILABLE CUT OFF
VOLUME/ TIME OF HANDLING/ RUNNING
LABORATORY SPECIMEN COLLECTION/ STORAGE DAY AND TAT FEE
QUANTITY COLLECTION TRANSPORT DAY
PROCEDURE TIME
700.00
sample on the
Package C/TEM Transport in Proper
Tissue grid ready for -- -- Monday -- 1 day Non-
Viewing only container clinical
viewing
sample

700.00
sample on the
Package C/TEM Body fluids; Transport in Proper Non-
grid ready for -- -- Monday -- 1 day
Viewing only Biological samples container clinical
viewing
sample

Package D/TEM sample already 1,820.00

blocked Transport in Proper Non-
Tissue -- -- Monday -- 1 week
Viewing with (polymerized container clinical
Micrograph resin)
sample

Package D/TEM sample already 1,820.00

Body fluids; blocked Transport in Proper
-- -- Monday -- 1 week Non-
Viewing with Biological samples (polymerized container clinical
Micrograph resin) sample

3,500.00
(Non-
clinical
Negative staining
Body fluids; Upon biopsy or autopsy transport sample)
with No fixative -- -- Monday -- 1 week
Biological samples immediately to the lab in ice pack
photomicrograph 8,500.00
(Clinical
sample)

Page 25 of 64
SPECIAL LABORATORY TESTS: BACTERIOLOGIC TESTS
AVAILABLE CUT OFF
VOLUME/ TIME OF HANDLING/ RUNNING
LABORATORY SPECIMEN COLLECTION/ STORAGE DAY AND TAT FEE
QUANTITY COLLECTION TRANSPORT DAY
PROCEDURE TIME

Confirmation/
Transport as soon as
Re-identification
Pure fresh Isolate possible
of the isolate Not Applicable
(plated or tubed -- -- -- < 24 hours, RT; Daily 5-7 days 1,200.00
medium) > 24 hours, on ice
(Aerobic
packs
organisms)

Confirmation/Re-
identification of Transport as soon as
the isolate and Pure fresh Isolate possible
Not Applicable
Susceptibility Test (plated or tubed -- -- -- < 24 hours, RT; Daily 5-7 days 1,320.00
medium) > 24 hours, on ice
(Aerobic packs
organisms)

Pseudomembrane: can be
removed or swab beneath the
Unpreserved :
membrane or the inner portion.
Transport as soon as
Place in sterile tube or any sterile
possible; ≤ 2 hours, RT
container or inoculate/rub the
inner portion of the membrane
Preserved:
onto Blood Agar plate
Amies Transport
Culture and Medium / Blood Agar
piece/s of or As soon As the Throat swab (TS): swab the
Susceptibility Test Pseudomembrane/ Plate:
swabs from suspicion arises, tonsils, posterior pharynx and Not Applicable
Throat swab/ ≤ 24 hours, RT
pseudo- preferably prior to other inflamed area. Daily 5-7 days 1,680.00
for Nasopharyngeal (if BAP only is
membrane or 2 antimicrobial Place swabs in sterile test tube or
Corynebacterium swab (NPS) submitted, include the
Throat swabs therapy Amies TM.
diphtheriae remaining
pseudomembrane)
NPS: insert small swab
(dacron/rayon) into the posterior
Note: plate or tube
nasopharynx via the nose. Rotate
must be wrapped with
the swab for 5 sec. to absorb
sealing film or any
secretions.
adhesive tape.
Place swabs in sterile test tube or
Amies TM.

Page 26 of 64
 AVAILABLE CUT OFF
VOLUME/ TIME OF HANDLING/ RUNNING
LABORATORY SPECIMEN COLLECTION/ STORAGE DAY AND TAT FEE
QUANTITY COLLECTION TRANSPORT DAY
PROCEDURE TIME

Unpreserved:
A dacron or rayon swab is
Transport as soon as
inserted into the nostrils w/
possible, RT
gently downward pressure in the
Culture for B.
As soon as the lower nasal floor to the posterior
pertussis/ B. Preserved: Regan
Nasopharyngeal suspicion arises, wall of the nasopharynx. The
parapertussis Lowe < 24 hours, RT; Not Applicable
Swab 1-2 swabs preferably prior to swab is allowed to stay for a few Mon-Wed-Fri 7-10 days 2,500.00
> 24 hours, on ice
(NPS) antimicrobial seconds to saturate then remove
packs
therapy gently;
Swab (2) the collected sample;
Storage: Refrigerate
Place the 2 swabs (NPS/NPA) in
(2-8°C) prior to
Regan Lowe Transport Medium
transport

Unpreserved:
Transport as soon as
possible, RT
As soon as the
Culture for B. Preserved: Regan
Nasopharyngeal suspicion arises, Swab (2) the collected sample;
pertussis/ B. Lowe < 24 hours, RT; Not Applicable
Aspirate 1 ml preferably prior to Place the 2 swabs (NPS/NPA) in Mon-Wed-Fri 7-10 days 2,500.00
parapertussis > 24 hours, on ice
(NPA) antimicrobial Regan Lowe Transport Medium
packs
therapy
Storage: Refrigerate
(2-8°C) prior to
transport

Collection: see collection of NPS

above
As soon as the Transport on ice packs
Molecular test for Nasopharyngeal suspicion arises, TRANSPORT Medium: Place the 2 3-5
Not Applicable
B. pertussis/ B. Swab 1-2 swabs preferably prior to swabs in Regan Lowe Transport Storage: Freeze (- Mon-Wed-Fri working 4,000.00
parapertussis (NPS) antimicrobial Medium,if unavailable, use 20°C) prior to days
therapy Universal Transport Medium transport
(UTM) or Atypical Pneumonia
Transport Medium (APTM)

As soon as the Transport on ice packs

Molecular test for Nasopharyngeal suspicion arises, 3-5
Collect in a sterile screw-capped Not Applicable
B. pertussis/ B. Aspirate 1 ml preferably prior to Storage: Freeze (- Mon-Wed-Fri working 4,000.00
tube.
parapertussis (NPA) antimicrobial 20°C) prior to days
therapy transport

Page 27 of 64
 AVAILABLE CUT OFF
VOLUME/ TIME OF HANDLING/ RUNNING
LABORATORY SPECIMEN COLLECTION/ STORAGE DAY AND TAT FEE
QUANTITY COLLECTION TRANSPORT DAY
PROCEDURE TIME
Transport tubes at an
upright and steady
position, Room
Bedside collection only.
Temperature (RT)
1. Acquire 4 tubes of EMJH or
Korthof’s medium (to be
Storage: Store at RT,
requested from referral
in closed
Culture for laboratory)
within 10 days compartment (e.g.
Pathogenic 2. Label tubes 1, 2, 3 and 4. Not Applicable
Whole blood 3 - 5 ml after onset of cabinet) until ready for Mon to Fri 12 weeks 2,900.00
Leptospira spp. 3. Aseptically inoculate one (1)
symptoms transport.
drop of blood into tube 1, two (2)
(for specimen with
drops into tube 2, three (3) drops
Philippine Integrated
into tubes 3 and 4.
Disease Survey
4. Wrap with opaque material
(PIDSR) or RITM
(preferably an aluminum foil).
Outbreak referral
Form: no longer than 2
weeks)
Transport tubes at an
upright and steady
position, Room
Bedside collection only. Temperature (RT)
1. Acquire 2 tubes of EMJH or
Korthof’s medium (to be Storage: Store at RT,
requested from referral in closed
Culture for
5-10 days after laboratory). compartment (e.g.
Pathogenic Not Applicable
CSF 0.5 - 1 ml onset of 2. Aseptically inoculate 0.5 ml of cabinet) until ready for Mon to Fri 12 weeks 2,900.00
Leptospira spp.
symptoms sample inti each tube. If quantity transport.
is not sufficient, inoculate into (for specimen with
one tube only. Philippine Integrated
3. Wrap with opaque material Disease Survey
(preferably an aluminum foil). (PIDSR) or RITM
Outbreak referral
Form: no longer than 2
weeks)
Transport tubes at an
upright and steady
position, Room
1. Collect 10-20 ml of midstream
Temperature (RT)
Culture for urine into sterile leak-proof
2nd week up to 30
Pathogenic container. Not Applicable
Urine 10 - 20 ml days after onset of Storage: Store at RT, Mon to Fri 12 weeks 2,900.00
Leptospira spp. 2. Follow the inoculation
symptoms in closed
procedure of whole blood stated
compartment (e.g.
above for urine.
cabinet) until ready for
transport.
(for specimen with

Page 28 of 64
 AVAILABLE CUT OFF
VOLUME/ TIME OF HANDLING/ RUNNING
LABORATORY SPECIMEN COLLECTION/ STORAGE DAY AND TAT FEE
QUANTITY COLLECTION TRANSPORT DAY
PROCEDURE TIME
Philippine Integrated
Disease Survey
(PIDSR) or RITM
Outbreak referral
Form: no longer than 2
weeks)
Transport on cold
Serologic test for pack as soon as
Pathogenic 5-10 days after Transfer separated serum into possible
Serum (Acute Not Applicable 7 working
Leptospira spp. > 0.5 ml onset of sterile 2 ml screw-capped tube or Mon to Fri 2,900.00
Phase) days
symptoms properly sealed red top tube Storage: Freezer
(MAT). temperature (-20°C)
prior to transport

Transport on cold
Serologic test for pack as soon as
Pathogenic Serum 5-20 days after Transfer separated serum into possible
Not Applicable 7 working
Leptospira spp. (Convalescent > 0.5 ml onset of sterile 2 ml screw-capped tube or Mon to Fri 2,900.00
days
Phase) symptoms properly sealed red top tube Storage: Freezer
(MAT) temperature (-20°C)
prior to transport

Molecular test for within 10 days Transport at Room 3-5

Aseptically collect blood into Not Applicable
Pathogenic Whole blood 3 - 5 ml after onset of Temperature as soon Mon to Fri working 3,500.00
EDTA Tube (purple top)
Leptospira spp. symptoms as possible days

Transport on ice pack

as soon as possible
Molecular test for within 10 days Transfer separated serum into 3-5
Not Applicable
Pathogenic Serum >0.5 ml after onset of sterile 2 ml screw-capped tube or Mon to Fri working 3,500.00
Storage: Freezer
Leptospira spp. symptoms properly sealed red top tube days
temperature (-20°C)
prior to transport

Transport on ice pack

as soon as possible
Molecular test for within 10 days 3-5
Store into sterile 2 mL screw-
Pathogenic CSF 0.5 ml - 2 ml after onset of Mon to Fri Not Applicable working 3,500.00
capped tube Storage: Freezer
Leptospira spp. symptoms days
temperature (-20°C)
prior to transport

Page 29 of 64
 AVAILABLE CUT OFF
VOLUME/ TIME OF HANDLING/ RUNNING
LABORATORY SPECIMEN COLLECTION/ STORAGE DAY AND TAT FEE
QUANTITY COLLECTION TRANSPORT DAY
PROCEDURE TIME

Transport on ice pack

as soon as possible
Molecular test for 2nd week up to 30 3-5
Collect in sterile screw capped
Pathogenic Urine >10 - 20 ml days after onset of Mon to Fri Not Applicable working 3,500.00
tubes Storage: Freezer
Leptospira spp. symptoms days
temperature (-20°C)
prior to transport

Culture:
63 4,600.00
calendar per case
Refrigerate if cannot days; DST: inclusive
Collect 2 specimens in a sterile
Solid TB be transported 49 of AFB
Pulmonary 3-5 ml Spot or early transparent, 50 ml-capacity Monday to
Culture/DST for immediately. calendar smear;
(Sputum, BAL, /container x 2 morning specimen screw capped container. To Mon-Fri Friday (2:00:00
Mycobacterium Transport in ice days (7
NPA) specimen preferred forward to the laboratory as PM)
Tuberculosis following IATA weeks No Charge
FRESH as possible
procedure. after for DOTS
release of and PMDT
positive Program
culture)

Culture:
Refrigerate if cannot 63 4,600.00
be transported calendar per case
CSF keep at room temperature or immediately. days; DST: inclusive
Solid TB Extrapulmonary in an incubator(37 degrees Transport in ice 49 of AFB
3-5 ml Monday to
Culture/DST for (CSF, tissue Celsius)until ready for transport following IATA calendar smear
/container x 2 -- Mon-Fri Friday (2:00:00
Mycobacterium exudates, skin to the laboratory; Gastric aspirate procedure. CSF -to days (7
specimen PM)
Tuberculosis etc.) should be delivered promptly to send the specimen weeks No Charge
the laboratory within 4 hours. IMMEDIATELY in after for DOTS
transport box release of and PMDT
WITHOUT ICE. positive Program
culture)

Page 30 of 64
 AVAILABLE CUT OFF
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LABORATORY SPECIMEN COLLECTION/ STORAGE DAY AND TAT FEE
QUANTITY COLLECTION TRANSPORT DAY
PROCEDURE TIME

Molecular test for

NDM1
Molecular test for
Pure isolate of
ESBL Monday to 3 working
E.coli/K. n/a Nutrient Agar slant/butt -- Mon-Thu -- 4,000.00
Thursday days
pneumoniae
(E. coli / K.
pneumoniae)

Molecular test for

mecA Pure isolate of
Monday to 3 working
E.coli/K. n/a Nutrient Agar slant/butt -- Mon-Thu -- 4,000.00
Thursday days
(Staphylococcus pneumoniae
aureus)

Not Applicable
Molecular test for Collection: see collection of NPS
Atypical above Transport in cold
Pneumonia: As soon as the chain, on ice packs
Legionella Nasopharyngeal suspicion arises, TRANSPORT Medium: Regan within 72 hours 3-5
pneumophila, Swab 1 swab preferably prior to Lowe Transport Medium, if Mon-Wed-Fri working 4,000.00
Mycoplasma (NPS) antimicrobial unavailable, use Universal Storage: Freeze (- days
pneumoniae and therapy Transport Medium (UTM) or 20°C) prior to
Chlamydophila Atypical Pneumonia Transport transport
pneumoniae Medium (APTM)

Not Applicable
Molecular test for
Atypical Transport in cold
Pneumonia: As soon as the chain, on ice packs
Legionella Nasopharyngeal suspicion arises, within 72 hours 3-5
Collect in a sterile screw-capped
pneumophila, Aspirate 1 ml preferably prior to Mon-Wed-Fri working 4,000.00
tube.
Mycoplasma (NPA) antimicrobial Storage: Freeze (- days
pneumoniae and therapy 20°C) prior to
Chlamydophila transport
pneumoniae

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PROCEDURE TIME

Transport in cold
Not Applicable
chain,< 72 hours, on
ice packs
Molecular test for Note: during
Haemophillus As soon as the weekends,
Storage: Freeze (-
influenzae, suspicion arises, holidays and 3-5
Collect in a sterile screw-capped 20°C) prior to
Neisseria CSF 0.5 ml - 2 ml preferably prior to Mon to Fri after office working 5,000.00
tube/vial transport (If culture is
meningitidis, antimicrobial hours days
requested, take a
Streptococcus therapy specimens are
separate sterile
pneumoniae received in the
container that will be
clinical
transported at room
laboratory
temperature)

Not Applicable

Molecular test for Transport in cold Note: during

Haemophillus As soon as the chain,< 72 hours, on weekends,
influenzae, suspicion arises, ice packs holidays and 3-5
Collect in red top tube only; place
Neisseria Serum 0.5 ml - 2 ml preferably prior to Mon to Fri after office working 5,000.00
serum in a sterile vial/tube.
meningitidis, antimicrobial Storage: Freeze (- hours days
Streptococcus therapy 20°C) prior to specimens are
pneumoniae transport received in the
clinical
laboratory

Transport in cold
Not Applicable
chain,<72 hours, on
ice pack
Note: during
As soon as the weekends,
Storage: Refrigerate
Molecular test for suspicion arises, holidays and 3-5
Collect in a sterile screw-capped (2-8°C) prior to
Neisseria CSF 0.5 ml - 2 ml preferably prior to Mon to Fri after office working 5,000.00
tube/vial transport (If culture is
meningitidis antimicrobial hours days
requested, take a
therapy specimens are
separate sterile
received in the
container that will be
clinical
transported at room
laboratory
temperature)

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PROCEDURE TIME

Not Applicable

Transport in cold Note: during

As soon as the chain,< 72 hours, on weekends,
Molecular test for suspicion arises, ice packs holidays and 3-5
Collect in red top tube only; place
Neisseria Serum 0.5 ml - 2 ml preferably prior to Mon to Fri after office working 5,000.00
serum in a sterile vial/tube.
meningitidis antimicrobial Storage: Freeze (- hours days
therapy 20°C) prior to specimens are
transport received in the
clinical
laboratory

Pulsed Field Gel

Electrophoresis Pure isolate of
(PFGE) Salmonellae/Shige
Salmonellae/Shige llae/Methicillin Monday to 3 working
n/a Nutrient Agar slant/butt -- Mon-Thu -- 4,500.00
llae/Methicillin resistant S. Thursday days
resistant S. aureus& V. cholera
aureus/V. cholera 01
01

Not Applicable
Transport
immediately, RT; Note: during
≤ 1 hour, RT; weekends,
Upon request, 1,200.00
Cryptococcal > 1 hour, transport on holidays and
preferably prior to Collect aseptically into a sterile
Antigen detection CSF 1 ml ice packs Mon-Thu after office 3 days
antifungal vial STAT
test (Qualitative) hours
treatment 1,700.00
Storage: specimens are
≥ 1 hour, freeze prior received in the
to transport clinical
laboratory

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PROCEDURE TIME

Not Applicable
Transport
immediately, RT; Note: during
≤ 1 hour, RT; weekends,
Upon request, 1,200.00
Cryptococcal > 1 hour, transport on holidays and
preferably prior to Collect aseptically into a sterile
Antigen detection serum 0.5 ml ice packs Mon-Thu after office 3 days
antifungal vial STAT
test (Qualitative) hours
treatment 1,700.00
Storage: specimens are
≥ 1 hour, freeze prior received in the
to transport clinical
laboratory

5 dilutions
Not Applicable
4,000.00
Transport
immediately, RT; Note: during
STAT
≤ 1 hour, RT; weekends,
Upon request, 4,500.00
Cryptococcal > 1 hour, transport on holidays and
preferably prior to Collect aseptically into a sterile
Antigen detection CSF 1 ml ice packs Mon-Thu after office 3 days
antifungal vial 10
test (Quantitative) hours
treatment dilutions
Storage: specimens are
6,000.00
≥ 1 hour, freeze prior received in the
to transport clinical
STAT
laboratory
6,500.00

5 dilutions
Not Applicable
4,000.00
Transport
immediately, RT; Note: during
STAT
≤ 1 hour, RT; weekends,
Upon request, 4,500.00
Cryptococcal > 1 hour, transport on holidays and
preferably prior to Collect aseptically into a sterile
Antigen detection serum 0.5 ml ice packs Mon-Thu after office 3 days
antifungal vial 10
test (Quantitative) hours
treatment dilutions
Storage: specimens are
6,000.00
≥ 1 hour, freeze prior received in the
to transport clinical
STAT
laboratory
6,500.00

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PROCEDURE TIME

Not Applicable
Transport as soon as
possible
Note: during
< 24 hours, RT;
Serotyping Other Isolate of weekends,
> 24 hours, on ice
Diarrheagenic E. Diarrheagenic E. holidays and
Pure, fresh subculture on Nutrient packs
coli: coli: -- n/a Mon-Fri after office 3-5 days 3,000.00
agar
(ETEC/EPEC/EHEC (ETEC/EPEC/EHEC hours
Storage:
/EIEC) /EIEC) specimens are
>24 hours, refrigerate
received in the
(4-8oC) prior to
clinical
transport
laboratory

Not Applicable
Transport as soon as
possible
Note: during
< 24 hours, RT;
weekends,
> 24 hours, on ice
holidays and
Serotyping Isolate of Pure, fresh subculture on Nutrient packs
-- n/a Mon-Fri after office 2-5 days 3,500.00
Salmonella spp. Salmonella spp. agar
hours
Storage:
specimens are
>24 hours, refrigerate
received in the
(4-8oC) prior to
clinical
transport
laboratory

Not Applicable
Transport as soon as
possible
Note: during
< 24 hours, RT;
weekends,
> 24 hours, on ice
holidays and
Serotyping Isolate of Shigella Pure, fresh subculture on Nutrient packs
-- n/a Mon-Fri after office 2-3 days 1,500.00
Shigella spp. spp. agar
hours
Storage:
specimens are
>24 hours, refrigerate
received in the
(4-8oC) prior to
clinical
transport
laboratory

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PROCEDURE TIME

Bacterial Antigen
Detection (BAD)

Streptococcus B;
As soon as the
Haemophilus
suspicion arises,
influenzae b; Collect aseptically into a sterile Transport
Body fluids 1-3 ml preferably prior to Mon-Wed-Fri Not Applicable 1 day 4,500.00
Streptococcus vial. immediately, RT;
antimicrobial
pneumoniae;
therapy
Neisseria
meningitidis;
Escherichia coli
K1

Not Applicable
Transport as soon as
possible
Note: during
< 24 hours, RT;
weekends,
> 24 hours, on ice
holidays and
Serotyping Vibrio Isolate of Vibrio Pure, fresh subculture on Nutrient packs
-- n/a Mon-Fri after office 2-3 days 1,320.00
cholerae 1 spp. agar
hours
Storage:
specimens are
>24 hours, refrigerate
received in the
(4-8oC) prior to
clinical
transport
laboratory

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SPECIAL LABORATORY TESTS: MYCOLOGY TESTS
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QUANTITY COLLECTION TRANSPORT DAY
PROCEDURE TIME
Preliminar
y report:
after 1
week

Final
report:Aft
er 4
Collection: Cleanse the skin with weeks if
If the suspected alcohol then disinfect with iodine there is no
1-2 ml (If
Fungal Culture causative agent or chlorhexidine prior to ≤ 2 hours, Room fungus
commercial
(request must be comes from the collecting blood sample. Temperature (RT); isolated
Blood bottle: follow Daily Not Applicable 2,500.00
accompanied by active infective (some
manufacturer’s
clinical abstract) site prior to BHI broth or Blood Culture Broth (BCB) ≤ 24 hours, RT may
instructions.)
antifungal therapy (BCB) is available at the Micro lab require up
/ Clinical lab.) to six (6)
weeks of
incubatio
n
depending
on the
clinical
impressio
n)

Preliminar
y report:
after 1
If the suspected
Collect aseptically in heparinized week
Fungal Culture causative agent ≤ 2 hours, Room
as much as tube or heparinize syringe and
(request must be Bone Marrow comes from the Temperature (RT);
possible or as in place in a sterile container; or in Daily Not Applicable Final 2,500.00
accompanied by Aspirate active infective
Blood specimen BHI broth or Blood Culture Broth report:Aft
clinical abstract) site prior to (BCB) ≤ 24 hours, RT er 4
(BCB) and a smear for gram stain
antifungal therapy weeks if
there is no
fungus
isolated

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QUANTITY COLLECTION TRANSPORT DAY
PROCEDURE TIME
(some
may
require up
to six (6)
weeks of
incubatio
n
depending
on the
clinical
impressio
n)

Preliminar
y report:
after 1
week

Final
report:Aft
er 4
Collection: Clean the surface or weeks if
Abscess drainage, If the suspected top of the infected area by there is no
Fungal Culture exudates, wound causative agent blotting with alcohol pad or with ≤ 2 hours, Room fungus
(request must be discharge or pus, Aspirates: 0.5-1 comes from the NSS prior to collection and place Temperature (RT); isolated
Daily Not Applicable 2,500.00
accompanied by drainage (note: mL; or 2 swabs active infective in a sterile container; if the (some
clinical abstract) aspirate is site prior to specimen is scanty, moisten the ≤ 24 hours, RT may
preferable) antifungal therapy swab with sterile NSS before require up
collection. to six (6)
weeks of
incubatio
n
depending
on the
clinical
impressio
n)

Fungal Culture Collection is done by physicians. ≤ 15 min, Room Preliminar

If the suspected
(request must be Eye (note: aspirate as much as Corneal scrapings: Specimen Temperature (RT); y report:
causative agent Daily Not Applicable 2,500.00
accompanied by is preferable) possible should be inoculated directly to after 1
comes from the
clinical abstract) the culture media. ≤ 24 hours, RT week
active infective

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PROCEDURE TIME
site prior to Vitreous humor: place in a sterile Final
antifungal therapy container report:Aft
er 4
weeks if
there is no
fungus
isolated
(some
may
require up
to six (6)
weeks of
incubatio
n
depending
on the
clinical
impressio
n)

Preliminar
y report:
after 1
week

See collection of specimen for Final

aerobic culture report:Aft
er 4
If the suspected Transport as soon as
Endocervical, Unpreserved: sterile container; weeks if
Fungal Culture causative agent possible;
vaginal /male there is no
(request must be 2 swabs and 1-2 comes from the (Unpreserved) ≤ 2 fungus
urethral/penile Preserved: Place one swab in Daily Not Applicable 2,500.00
accompanied by smears active infective hours, RT;
discharge/anorect Amies Transport Medium; and the isolated
clinical abstract) site prior to (Preserved) ≤ 24
al swab other swab to make a smear (air (some
antifungal therapy hours, RT may
dry the smear then cover with
another clean slide and wrap with require up
a clean paper) to six (6)
weeks of
incubatio
n
depending
on the
clinical

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PROCEDURE TIME
impressio
n)

Preliminar
y report:
after 1
week

Final
report:Aft
er 4
weeks if
If the suspected there is no
≤ 15 min., RT
Fungal Culture causative agent fungus
< 24 hours, RT,
(request must be Catheter tip comes from the Remove distal 3 to5 cm. of line isolated
-- refrigerate Daily Not Applicable 2,500.00
accompanied by (intravascular) active infective tip and place in a sterile container (some
(4-8oC) prior to
clinical abstract) site prior to may
transport
antifungal therapy require up
to six (6)
weeks of
incubatio
n
depending
on the
clinical
impressio
n)

Preliminar
y report:
Transport after 1
If the suspected immediately; week
Sterile body
Fungal Culture causative agent Collect into a sterile container or
fluids:CSF,
(request must be comes from the CSF in plain BHI broth; other body Unpreserved CSF: ≤ Final
pericardial, 1-3 mL Daily Not Applicable report: 2,500.00
accompanied by active infective fluids in heparinized tubes. 15 min., RT;
synovial or
clinical abstract) site prior to Specify fungal species suspected After 4
peritoneal fluids weeks if
antifungal therapy Heparinized specimen:
≤ 24 hours, RT there is no
fungus
isolated

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QUANTITY COLLECTION TRANSPORT DAY
PROCEDURE TIME
(some
may
require up
to six (6)
weeks of
incubatio
n
depending
on the
clinical
impressio
n)

Preliminar
y report:
Cleanse the affected area with after 1
70% alcohol and allow to dry prior week
to collection.
Final
Skin: Scrape the edge of the skin report:
lesion with a dull edge of the After 4
scalpel or alcohol sterilized glass weeks if
slide and place in a sterile there is no
If the suspected
container. Transport as soon as fungus
Fungal Culture Skin scrapings; causative agent
possible; ≤ 3 days, RT isolated
(request must be Nail, or shavings as much as comes from the
Nail: small clippings or scrape the Daily Not Applicable 2,500.00
accompanied by or debris under possible active infective
top or under the nail with a (Note: Never (some
clinical abstract) nail; Hair site prior to
scalpel. refrigerate) may
antifungal therapy
require up
Hair: pluck 10-12 infected hairs. to six (6)
weeks of
Note: All specimens must be incubatio
placed in a dry, sterile container n
or in a clean dry paper envelope depending
or place between 2 glass slides on the
wrapped with clean paper. clinical
impressio
n)

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PROCEDURE TIME
Preliminar
y report:
after 1
week

Final
report:
(Sputum) < 2 hours, After 4
Sputum: Collect first morning RT; weeks if
specimen after brushing of teeth (BAL fluid) < 24 hours, there is no
If the suspected
RT fungus
Fungal Culture Respiratory tract, causative agent
Bronchial aspirates: surgically isolated
(request must be lower: Sputum, comes from the
1-5 mL collect brushings. If longer, wrapped the Daily Not Applicable 2,500.00
accompanied by bronchial aspirates active infective
specimen container in (some
clinical abstract) and BAL fluid site prior to
BAL fluid: surgically collected plastic /cellophane may
antifungal therapy
Note: place all samples in sterile and place on cold pack require up
leak-proof container or if not transported to six (6)
on same day. weeks of
incubatio
n
depending
on the
clinical
impressio
n)

Preliminar
y report:
Transport
after 1
immediately;
week

If the suspected Swab oral lesions; Use flexible If longer:

Respiratory, upper: Final
Fungal Culture causative agent swab for oropharynx; sinus
Oral swab, Swabs: 2 report:
(request must be comes from the content is surgically collected Oral/oropharyngeal: After 4
oropharyngeal Sinus content: Daily Not Applicable 2,500.00
accompanied by active infective and place in a sterile container or ≤ 2 hours, RT; ≤ 24
swab, and sinus 1-5 mL weeks if
clinical abstract) site prior to in Amies TM. Specify suspected hours, RT
samples there is no
antifungal therapy fungal species fungus
Sinus sample: ≤ 15 isolated
min., RT; < 24 hours,
RT (some
may
require up

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PROCEDURE TIME
to six (6)
weeks of
incubatio
n
depending
on the
clinical
impressio
n)

Final
report:
After 4
weeks if
there is no
fungus
isolated
Fungal Culture
Scrape throat/mouth with a Some may
(request must be Throat/Mouth as much as preferably prior to ≤ 2 hours, RT; ≤ 24
sterile tongue depressor and Daily Not Applicable require up 2,500.00
accompanied by scrapings possible antifungal therapy hours, RT
place in a sterile container to six (6)
clinical abstract)
weeks of
incubatio
n
depending
on the
clinical
impressio
n

Final
report:
Stool (stool
After 4
sample should be weeks if
discouraged,
Fungal Culture there is no
tissue biopsy of GI Transport as soon as
(request must be preferably prior to Collect into a sterile container. fungus
tract is the Thumb size possible; ≤ 2 hours, Daily Not Applicable 2,500.00
accompanied by antifungal therapy Specify fungal species suspected isolated
preferred RT
clinical abstract)
specimen if
Some may
available, see
require up
tissue) to six (6)
weeks of
incubatio

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PROCEDURE TIME
n
depending
on the
clinical
impressio
n

Final
report:
After 4
weeks if
there is no
fungus
≤ 2 hours, RT; ≤ 24
isolated
First morning clean catch or hours, on ice packs
Fungal Culture subrapubic or catheterized (see
Urine (24-hour Some may
(request must be preferably prior to collection of urine for aerobic (wrap the container in
specimens are 5-10 ml Daily Not Applicable require up 2,500.00
accompanied by antifungal therapy culture) urine in a sterile leak- a plastic
unacceptable) to six (6)
clinical abstract) proof container. Specify wrapper/cellophane
weeks of
suspected fungal species and place with ice
incubatio
packs)
n
depending
on the
clinical
impressio
n

Final
Unpreserved: report:
Collected during surgery or Transport as soon as After 4
cutaneous biopsy procedure: possible; weeks if
there is no
-place in sterile leak-proof Preserved: fungus
Fungal Culture ≤ 24 hours., RT isolated
container.
(request must be as much as preferably prior to
Tissue >24 hours, on ice Daily Not Applicable 2,500.00
accompanied by possible antifungal therapy
Unpreserved: Dry sterile container packs Some may
clinical abstract) (see note above) require up
Preserved: If small specimen add to six (6)
few drops of sterile NSS; If Storage: weeks of
bigger, 0.5-1 ml in sterile NSS ≤ 24 hours., RT; >24 incubatio
hours, refrigerate (4- n
8oC) prior to transport depending
on the

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PROCEDURE TIME
suspend in 0.5-1 ml clinical
in sterile NSS impressio
n

Isolates of
Candida spp.,
Antifungal
Cryptococcus spp.,
Susceptibility Test
Aspergillus spp. See all specimen for
(including re- -- -- -- Tue-Thu Not Applicable 1 week 1,950.00
and other fungal culture
identification of
miscellaneous
isolates)
rapid growing
yeast spp.

CSF and all Fluids, Exudates:

India ink possible 0.5-3 ml
Prior to antifungal See all specimen for fungal See all specimen for
specimens for the Tissue: as much Daily Not Applicable 1 day 170.00
therapy culture fungal culture
(Microscopy) detection of as possible
Cryptocccus sp. Sputum: 1-5 ml

See all specimen for

Fluids, Exudates:
fungal culture
0.5-3 ml;
Potassium Tissue: as much
(If the specimen was
Hydroxide All clinical as possible; Prior to antifungal See all specimen for fungal
already placed on a Daily Not Applicable 1 day 170.00
specimens Sputum: therapy culture
slide, cover with
(Microscopy) 1-5 ml; Skin: as
another clean slide
much as
and wrap in a clean
possible
paper)

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SPECIAL LABORATORY TESTS: VIROLOGIC TESTS
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PROCEDURE TIME

VIRUS PANEL TESTING

Immediately
Molecular test for refrigerate at 2-8
· Collect in plain or red top tube degree Celsius while
Panel 1 (CNS): Upon first contact · Allow to clot awaiting transport
Serum 1 ml Tue/Thu Mon/Wed 7 days 4,000.00
Japanese with the patient · Centrifuge
Encephalitis + · Separate serum from blood Transport in ice to be
West Nile Virus received at RITM
within 72 hours
Immediately
refrigerate at 2-8
degree Celsius while
Molecular test for awaiting transport

Panel 1 (CNS): Upon first contact Collect aseptically in a sterile Transport in ice to be
CSF 1 ml Tue/Thu Mon/Wed 7 days 4,000.00
Japanese with the patient glass/plastic container received at RITM
Encephalitis + within 72 hours
West Nile Virus
Place container in a
ziplock/leakproof
plastic bags
Immediately
refrigerate at 2-8
degree Celsius while
Molecular test for
awaiting transport
Panel 2 (CNS):
Upon first contact Collect aseptically in a sterile Transport in ice to be
Japanese CSF 1 ml Tue/Thu Mon/Wed 7 days 5,000.00
with the patient glass/plastic container received at RITM
Encephalitis +
within 72 hours
West Nile Virus +
Enterovirus
Place container in a
ziplock/leakproof
plastic bags

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PROCEDURE TIME
Immediately
Molecular test for
refrigerate at 2-8
· Collect in plain or red top tube degree Celsius while
Panel 2 (CNS):
Upon first contact · Allow to clot awaiting transport
Japanese Serum 1 ml Tue/Thu Mon/Wed 7 days 5,000.00
with the patient · Centrifuge
Encephalitis +
· Separate serum from blood Transport in ice to be
West Nile Virus +
received at RITM
Enterovirus
within 72 hours
Immediately
refrigerate at 2-8
degree Celsius while
awaiting transport
Molecular test for
Upon first contact Collect aseptically in a sterile Transport in ice to be
CSF 1 ml Tue/Thu Mon/Wed 7 days 5,000.00
Panel 3 (CNS): with the patient glass/plastic container received at RITM
HSV-1 & 2, CMV within 72 hours

Place container in a
ziplock/leak-proof
plastic bags
Immediately
refrigerate at 2-8
Molecular test for degree Celsius while
awaiting transport
Panel 4 (CNS):
West Nile Virus, Upon first contact Collect aseptically in a sterile Transport in ice to be
CSF 1 ml Tue/Thu Mon/Wed 7 days 8,000.00
Japanese with the patient glass/plastic container received at RITM
Encephalitis, within 72 hours
Enteroviruse, HSV
1&2, CMV Place container in a
ziplock/leak-proof
plastic bags
Rapid Test: IF
Immediately
Panel 5 (RESPI):
refrigerate at 2-8
Flu/Respiratory 1,500.00
degree Celsius while
viruses panel: Within 5 days from · Collect in a mucus per target
awaiting transport
Influenza A&B NPA 1 ml onset of trap/extractor Mon-Fri Tue-Thu 7 days
RSV symptoms · Suspend in VTM/UTM 5,000.00
Transport in ice to be
Parainfluenza Al targets
received at RITM
1,2,3
within 72 hours
Adenovirus
HSV 1 & 2

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PROCEDURE TIME
Virus isolation

Panel 5 (RESPI):
Flu/Respiratory Immediately
viruses panel: refrigerate at 2-8
Influenza A&B degree Celsius while
Within 5 days from · Use Dacron or rayon swabs with
RSV awaiting transport
NPS/OPS 2 swabs onset of plastic shafts MWF TTh 3 weeks 5,000.00
Parainfluenza
symptoms · Suspend in VTM/UTM
1,2,3 Transport in ice to be
Rhinovirus received at RITM
Adenovirus within 72 hours
Enterovirus
HSV 1 & 2

Virus isolation

Panel 5 (RESPI):
Flu/Respiratory Immediately
viruses panel: refrigerate at 2-8
Influenza A&B degree Celsius while
Within 5 days from · Collect in a mucus
RSV awaiting transport
NPA 2 ml onset of trap/extractor MWF TTh 3 weeks 5,000.00
Parainfluenza
symptoms · Suspend in VTM/UTM
1,2,3 Transport in ice to be
Rhinovirus received at RITM
Adenovirus within 72 hours
Enterovirus
HSV 1 & 2

Virus isolation

Panel 5 (RESPI):
Flu/Respiratory Immediately
viruses panel: refrigerate at 2-8
Influenza A&B degree Celsius while
Within 5 days from · Collect in a mucus
RSV awaiting transport
Lung aspirate 1 ml onset of trap/extractor MWF TTh 3 weeks 5,000.00
Parainfluenza
symptoms · Suspend in VTM/UTM
1,2,3 Transport in ice to be
Rhinovirus received at RITM
Adenovirus within 72 hours
Enterovirus
HSV 1 & 2

Page 48 of 64
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LABORATORY SPECIMEN COLLECTION/ STORAGE DAY AND TAT FEE
QUANTITY COLLECTION TRANSPORT DAY
PROCEDURE TIME
Immediately
Molecular test for
refrigerate at 2-8
degree Celsius while
Panel 6 (RESPI): Within 5 days from · Use Dacron or rayon swabs with
awaiting transport
Metapneumovirus NPS/OPS 2 swabs onset of plastic shafts Tue/Thu Mon/Wed 7 days 4,000.00
+ Respiratory symptoms · Suspend in VTM/UTM
Transport in ice to be
syncytial virus
received at RITM
(RSV)
within 72 hours
Immediately
Molecular test for
refrigerate at 2-8
degree Celsius while
Panel 6 (RESPI): Within 5 days from · Collect in a mucus
awaiting transport
Metapneumovirus NPA 2 ml onset of trap/extractor Tue/Thu Mon/Wed 7 days 4,000.00
+ Respiratory symptoms · Suspend in VTM/UTM
Transport in ice to be
syncytial virus
received at RITM
(RSV)
within 72 hours
Immediately
Molecular test for
refrigerate at 2-8
degree Celsius while
Panel 6 (RESPI): Within 5 days from · Collect in a mucus
awaiting transport
Metapneumovirus Lung aspirate 1 ml onset of trap/extractor Tue/Thu Mon/Wed 7 days 4,000.00
+ Respiratory symptoms · Suspend in VTM/UTM
Transport in ice to be
syncytial virus
received at RITM
(RSV)
within 72 hours
Immediately
refrigerate at 2-8
Molecular test for degree Celsius while
Within 5 days from · Use Dacron or rayon swabs with
awaiting transport
NPS/OPS 2 swabs onset of plastic shafts Tue/Thu Mon/Wed 7 days 5,000.00
Panel 7 (RESPI):
symptoms · Suspend in VTM/UTM
RSV, MPV, ADV Transport in ice to be
received at RITM
within 72 hours
Immediately
refrigerate at 2-8
Molecular test for
degree Celsius while
Within 5 days from · Use Dacron or rayon swabs with
awaiting transport
Panel 8 (RESPI): NPS/OPS 2 swabs onset of plastic shafts Tue/Thu Mon/Wed 7 days 5,000.00
Parainfluenza 1-3, symptoms · Suspend in VTM/UTM
Transport in ice to be
EV
received at RITM
within 72 hours

Page 49 of 64
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LABORATORY SPECIMEN COLLECTION/ STORAGE DAY AND TAT FEE
QUANTITY COLLECTION TRANSPORT DAY
PROCEDURE TIME
Immediately
refrigerate at 2-8
Molecular test for
degree Celsius while
Panel 9 (RESPI): Within 5 days from · Use Dacron or rayon swabs with
awaiting transport
RSV, MPV, ADV, NPS/OPS 2 swabs onset of plastic shafts Tue/Thu Mon/Wed 7 days 8,500.00
Parainfluenza 1-3, symptoms · Suspend in VTM/UTM
Transport in ice to be
EV
received at RITM
within 72 hours
Immediately
refrigerate at 2-8
degree Celsius while
Molecular test for awaiting transport
Adult thumb Within 14 days
Panel 10 (GI): Collect in sterile / clean screw- Transport in ice to be
Stool size/5-10 ml from onset of Tuesday Monday 10 days 5,500.00
Rotavirus, capped container received at RITM
liquid stool diarrhea
Norovirus within 72 hours
Genogroup 1 & 2
Place container in a
ziplock/leakproof
plastic bags
Immediately
refrigerate at 2-8
degree Celsius while
awaiting transport
Molecular test for
Adult thumb Within 14 days
Collect in sterile / clean screw- Transport in ice to be
Panel 11 (GI): Stool size/5-10 ml from onset of Tue/Thu Mon/Wed 10 days 5,000.00
capped container received at RITM
Adeno virus, liquid stool diarrhea
within 72 hours
Enterovirus
Place container in a
ziplock/leakproof
plastic bags
Immediately
refrigerate at 2-8
degree Celsius while
Molecular test for
awaiting transport
Panel 12 (GI): Adult thumb Within 14 days
Collect in sterile / clean screw- Transport in ice to be
Rotavirus, Stool size/5-10 ml from onset of Tue/Thu Mon/Wed 10 days 8,500.00
capped container received at RITM
Norovirus, liquid stool diarrhea
within 72 hours
Adenovirus,
Enterovirus
Place container in a
ziplock/leakproof
plastic bags

Page 50 of 64
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LABORATORY SPECIMEN COLLECTION/ STORAGE DAY AND TAT FEE
QUANTITY COLLECTION TRANSPORT DAY
PROCEDURE TIME

RABIES VIRUS

Serologic

5 days
Use protective
after a
clothing (gloves,
batch test
Tissue samples must be fresh or gown, apron, face
(Batch
frozen and should not be mask and or face Mon-Thu
Molecular test for Approximately testing
Brain Tissue Post mortem chemically preserved. Cooked, shield) (except -- 5,000.00
Rabies virus 30 mm x 3 mm done
burned and autolyzed samples -20°C if testing is holidays)
every 10th
will be rejected. delayed. 2°C - 8°C if to
and 25th
be tested immediately.
day of the
Transport in ice.
month)
5 days
Use protective
after a
clothing (gloves,
batch test
0.5 ml saliva collected serially by gown, apron, face
Upon appearance (Batch
aspiration, every 4-6 hours. 3-5 mask and or face Mon-Thu
Molecular test for 5 ml per of rabies specific testing
Saliva sample collections pooled & shield) (except -- 5,000.00
Rabies virus collection signs and done
aliquoted for antemortem/tests & -20°C if testing is holidays)
symptoms every 10th
specimen banking. delayed. 2°C - 8°C if to
and 25th
be tested immediately.
day of the
Transport in ice.
month)
Put tissue in a tube or
specimen vial then
place the vial into a
leak-proof secondary
72
container, then again
working
Wear proper personal protective in a tertiary leak-proof
hours
Rabies Antigen clothing (gown, double gloves, packaging (e.g. Mon-Fri No charge
upon
Detection by As soon as the mask, face shield or eye Ziploc). Lastly, place (except until
receipt by
direct patient died, with protection). Can be collected in a sturdy outer holidays), further
Brain Tissue 1 cm x 1 cm -- the
FLUORESCENT consent from the without opening the skull, via the packaging (e.g. acceptance of notice
Special
ANTIBODY TEST relative atlanto-occipital joint. Must be cooler) for transport. specimen is 24
Pathogens
(dFAT) stored frozen or buffered glycerol Frozen brain tissue hours
Laborator
(1:1 glycerol and normal saline). must be transported to
y (VRD-
the laboratory
SPL)
immediately. Dry ice
may be used for longer
transport. Tissue
stored in buffered

Page 51 of 64
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QUANTITY COLLECTION TRANSPORT DAY
PROCEDURE TIME
glycerol must also be
transported with ice.

MEASLES and other EXANTHEMS

Immediately
refrigerate at 2-8
· Collect in plain or red top tube or
degree Celsius while
Serology: IgM yellow SST
Within 4-28 days awaiting transport
Serum 2 ml · Allow to clot Mon-Fri -- 7 days 1,500.00
from onset of rash
Measles Virus · Centrifuge
Transport in ice to be
· Separate serum from blood
received at RITM
within 72 hours
Immediately
refrigerate at 2-8
degree Celsius while
· Use Dacron or rayon swabs with
Molecular test for Within 7 days from awaiting transport
NPS/OPS 2 swabs plastic shafts Tuesday Monday 10 days 4,000.00
Measles Virus onset of rash
· Suspend in VTM/UTM
Transport in ice to be
received at RITM
within 72 hours
Immediately
refrigerate at 2-8
degree Celsius while
Virus isolation · Use Dacron or rayon swabs with
Within 7 days from awaiting transport
NPS/OPS 2 swabs plastic shafts Wednesday Tuesday 3 weeks 3,000.00
onset of rash
(Measles Virus) · Suspend in VTM/UTM
Transport in ice to be
received at RITM
within 72 hours
Immediately
refrigerate at 2-8
· Collect in plain or red top tube degree Celsius while
Serology: IgM/IgG
5 days after onset · Allow to clot awaiting transport
Serum 1 ml Thursday Wednesday 10 days 1,500.00
of fever · Centrifuge
Mumps
· Separate serum from blood Transport in ice to be
received at RITM
within 72 hours
Immediately
· Use Dacron or rayon swabs with refrigerate at 2-8
Molecular test for Upon first contact
Buccal swab 2 swabs plastic shafts degree Celsius while Tuesday Monday 10 days 4,000.00
Mumps w/ the patient
· Suspend in VTM/UTM awaiting transport

Page 52 of 64
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LABORATORY SPECIMEN COLLECTION/ STORAGE DAY AND TAT FEE
QUANTITY COLLECTION TRANSPORT DAY
PROCEDURE TIME
Transport in ice to be
received at RITM
within 72 hours

Place container in a
ziplock/leakproof
plastic bags
Immediately
refrigerate at 2-8
degree Celsius while
awaiting transport

Molecular test for >4 days onset of Collect first morning midstream Transport in ice to be
Urine 10 ml Tuesday Monday 10 days 4,000.00
Mumps fever in a sterile wide-mouth container received at RITM
within 72 hours

Place container in a
ziplock/leakproof
plastic bags
Immediately
refrigerate at 2-8
· Collect in plain or red top tube or
degree Celsius while
Serology: IgM yellow SST
Within 4-28 days awaiting transport
Serum 1-2 ml · Allow to clot Mon-Fri -- 7 days 1,500.00
from onset of rash
Rubella Virus · Centrifuge
Transport in ice to be
· Separate serum from blood
received at RITM
within 72 hours
Immediately
refrigerate at 2-8
degree Celsius while
· Use Dacron or rayon swabs with
Molecular test for Within 7 days from awaiting transport
NPS/OPS 2 swabs plastic shafts Tuesday Monday 10 days 4,000.00
Rubella Virus onset of rash
· Suspend in VTM/UTM
Transport in ice to be
received at RITM
within 72 hours
Immediately
refrigerate at 2-8
degree Celsius while
Virus isolation · Use Dacron or rayon swabs with
Within 7 days from awaiting transport
NPS/OPS 2 swabs plastic shafts Wednesday Tuesday 3 weeks 3,000.00
onset of rash
(Rubella Virus) · Suspend in VTM/UTM
Transport in ice to be
received at RITM
within 72 hours

Page 53 of 64
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LABORATORY SPECIMEN COLLECTION/ STORAGE DAY AND TAT FEE
QUANTITY COLLECTION TRANSPORT DAY
PROCEDURE TIME

DENGUE and other ARBOVIRUSES

Immediately
refrigerate at 2-8
· Collect in plain or red top tube. degree Celsius while
Molecular test for Within 5 days from · Allow to clot awaiting transport
Serum 1 ml Wednesday Monday AM 7 days 4,000.00
Dengue Virus onset of fever · Centrifuge
· Separate serum from blood Transport in ice to be
received at RITM
within 72 hours
Immediately
refrigerate at 2-8
degree Celsius while
awaiting transport
Serology: IgM
CSF and Serum Upon first contact Collect aseptically in a sterile Transport in ice to be
1 ml Tuesday Monday 10 days 1,500.00
Japanese (paired) w/ the patient glass/plastic container received at RITM
Encephalitis within 72 hours

Place container in a
ziplock/leak-proof
plastic bags
Immediately
refrigerate at 2-8
Serology: ELISA
· Collect in plain or red top tube. degree Celsius while
IgM
7 days after onset · Allow to clot awaiting transport
Serum 1 ml Thursday Wednesday 10 days 1,500.00
of fever · Centrifuge
Chikungunya
· Separate serum from blood Transport in ice to be
(CHIKV)
received at RITM
within 72 hours
Immediately
refrigerate at 2-8
· Collect in plain or red top tube. degree Celsius while
Molecular test for
7 days after onset · Allow to clot awaiting transport
Chikungunya Serum 1 ml Tue/Thu Mon/Wed 7 days 4,000.00
of fever · Centrifuge
(CHIKV)
· Separate serum from blood Transport in ice to be
received at RITM
within 72 hours

ROTAVIRUS and other ENTERIC VIRUSES

Page 54 of 64
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LABORATORY SPECIMEN COLLECTION/ STORAGE DAY AND TAT FEE
QUANTITY COLLECTION TRANSPORT DAY
PROCEDURE TIME
Immediately
refrigerate at 2-8
Serology: Antigen degree Celsius while
Adult thumb Within 14 days
Detection Collect in sterile / clean screw- awaiting transport
Stool size/5-10 ml from onset of -- -- 10 days 1,500.00
capped container
liquid stool diarrhea
Rotavirus Transport in ice to be
received at RITM
within 72 hours
Immediately
refrigerate at 2-8
degree Celsius while
Adult thumb Within 14 days
Molecular test for Collect in sterile / clean screw- awaiting transport
Stool size/5-10 ml from onset of -- -- 10 days 4,000.00
Rotavirus capped container
liquid stool diarrhea
Transport in ice to be
received at RITM
within 72 hours

Immediately
refrigerate at 2-8
degree Celsius while
Molecular test for Adult thumb Within 14 days
Collect in sterile / clean screw- awaiting transport
Norovirus Group 1 Stool size/5-10 ml from onset of Tuesday Monday 10 days 4,000.00
capped container
&2 liquid stool diarrhea
Transport in ice to be
received at RITM
within 72 hours

POLIOVIRUS and other ENTEROVIRUSES

Immediately
refrigerate at 2-8
degree Celsius while
awaiting transport
1 thumb sized or Within 14 days
Molecular test for Place in a clean screw- capped Transport in ice to be
Stool/Rectal swab 5 to 10 ml if from onset of Tuesday Monday 10 days 4,000.00
Enterovirus (EV) container received at RITM
diarrheic symptoms
within 72 hours

Place container in a
ziplock/leakproof
plastic bags
Within 7 to 10 Immediately
Molecular test for days after from Collect aseptically in a sterile refrigerate at 2-8
CSF 1 ml Tuesday Monday 10 days 4,000.00
Enterovirus (EV) onset of glass/plastic container degree Celsius while
symptoms awaiting transport

Page 55 of 64
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LABORATORY SPECIMEN COLLECTION/ STORAGE DAY AND TAT FEE
QUANTITY COLLECTION TRANSPORT DAY
PROCEDURE TIME

Transport in ice to be
received at RITM
within 72 hours

Place container in a
ziplock/leak-proof
plastic bags
Immediately
refrigerate at 2-8
degree Celsius while
awaiting transport
· Use Dacron or rayon swabs with
Molecular test for Within 5 days from Transport in ice to be
NPS/OPS 2 swabs plastic shafts Tuesday Monday 10 days 4,000.00
Enterovirus (EV) onset of fever received at RITM
· Suspend in VTM/UTM
within 72 hours

Place container in a
ziplock/leak-proof
plastic bags
Immediately
refrigerate at 2-8
degree Celsius while
awaiting transport
· Use Dacron or rayon swabs with
Molecular test for With presence of Transport in ice to be
Vesicular swab 2 swabs plastic shafts Tuesday Monday 10 days 4,000.00
Enterovirus (EV) unraptured vesicle received at RITM
· Suspend in VTM/UTM
within 72 hours

Place container in a
ziplock/leak-proof
plastic bags
Immediately
refrigerate at 2-8
degree Celsius while
Virus isolation awaiting transport
1 thumb sized or Within 14 days
Enterovirus (EV) Place in a clean screw- capped Transport in ice to be
Stool 5 to 10 ml if from onset of MWF Tue/Thu 2 weeks 3,000.00
1. Polioviruses container received at RITM
diarrheic paralysis
2. NPEV (Nonpolio within 72 hours
enteroviruses)
Place container in a
ziplock/leak-proof
plastic bags

Page 56 of 64
 AVAILABLE CUT OFF
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LABORATORY SPECIMEN COLLECTION/ STORAGE DAY AND TAT FEE
QUANTITY COLLECTION TRANSPORT DAY
PROCEDURE TIME

INFLUENZA and other RESPIRATORY VIRUSES

Immediately
refrigerate at 2-8
Molecular test for degree Celsius while
Within 5 days from · Use Dacron or rayon swabs with
Influenza A&B awaiting transport
NPS/OPS 2 swabs onset of plastic shafts Thursday Wednesday 10 days 4,000.00
with subtyping
symptoms · Suspend in VTM/UTM
(H1, H3) Transport in ice to be
received at RITM
within 72 hours
Immediately
refrigerate at 2-8
Molecular test for Endotracheal degree Celsius while
Within 7 days from
Influenza A&B Aspirate; Collect aseptically in a sterile awaiting transport
2 ml onset of Thursday Wednesday 10 days 4,000.00
with subtyping Bronchoalveolar glass/plastic container
symptoms
(H1, H3) lavage Transport in ice to be
received at RITM
within 72 hours
Immediately
refrigerate at 2-8
degree Celsius while
Adult thumb Within 14 days
Molecular test for Collect in sterile / clean screw- awaiting transport
Stool size/5-10 ml from onset of Tue/Thu Mon/Wed 7 days 3,000.00
Adenovirus (AdV) capped container
liquid stool diarrhea
Transport in ice to be
received at RITM
within 72 hours
Immediately
refrigerate at 2-8
degree Celsius while
· Use Dacron or rayon swabs with
Molecular test for Within 5 days from awaiting transport
NPS/OPS 2 swabs plastic shafts Tue/Thu Mon/Wed 7 days 3,000.00
Adenovirus (AdV) onset of fever
· Suspend in VTM/UTM
Transport in ice to be
received at RITM
within 72 hours

HUMAN IMMUNODEFICIENCY VIRUS (HIV)

HIV-1 Drug Transport in ice within
resistance Collect in 4-ml EDTA (purple- top) 24 hours Mon-Fri
Plasma > 2ml Anytime None 8 weeks 6,000.00
genotyping by tube. Freeze prior to transport after collection. Pack (batch testing)
sequencing and transport as

Page 57 of 64
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LABORATORY SPECIMEN COLLECTION/ STORAGE DAY AND TAT FEE
QUANTITY COLLECTION TRANSPORT DAY
PROCEDURE TIME
Category B infectious
substances (UN 3373)

*10AM for
scheduled
HIV-1 & HIV-3 Collect in 2 plastic tubes of 4ml
2 EDTA tube x 4 Mon-Fri (except Transport immediately Mon-Fri except clients 5 working
CD4 Whole blood EDTA, freshly collected specimen 3,000.00
ml Wed) at room temperature Wednesday *2PM for days
Molecular test is needed
referral and in-
patient
Collect in 2 plastic tubes of 4ml
HIV-1 & HIV-4 EDTA and separate plasma into 2
2 EDTA tube x 4 Freeze, transport with
Viral Load testing Plasma Mon-Fri cryotubes (1.5-2.5 mL) within 4 Mon-Fri -- 1 month 3,000.00
ml ice
Molecular test hours. Store at -20⁰C or below if
not transported immediately.

OTHER VIRUSES
Immediately
refrigerate at 2-8
degree Celsius while
awaiting transport
Molecular test for
Urine (Ideal Within 5 days from Collect first morning midstream Transport in ice to be
Cytomegalovirus 5-10 ml Tue/Thu Mon/Wed 7 days 4,000.00
sample) onset of fever in a sterile wide-mouth container received at RITM
(CMV)
within 72 hours

Place container in a
ziplock/leak-proof
plastic bags
Immediately
refrigerate at 2-8
degree Celsius while
awaiting transport
· Collect in purple/violet top
Molecular test for
Within 5 days from (EDTA) tube Transport in ice to be
Cytomegalovirus Whole blood 10 ml Tue/Thu Mon/Wed 7 days 4,000.00
onset of fever · Invert gently to mix received at RITM
(CMV)
anticoagulant within 72 hours

Place container in a
ziplock/leak-proof
plastic bags

Page 58 of 64
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VOLUME/ TIME OF HANDLING/ RUNNING
LABORATORY SPECIMEN COLLECTION/ STORAGE DAY AND TAT FEE
QUANTITY COLLECTION TRANSPORT DAY
PROCEDURE TIME
Immediately
refrigerate at 2-8
degree Celsius while
awaiting transport
Molecular test for
Within 5 days from Collect aseptically in a sterile Transport in ice to be
Cytomegalovirus CSF 1 ml Tue/Thu Mon/Wed 7 days 4,000.00
onset of fever glass/plastic container received at RITM
(CMV)
within 72 hours

Place container in a
ziplock/leak-proof
plastic bags
Immediately
refrigerate at 2-8
degree Celsius while
awaiting transport
Molecular test for
1 thumb sized or Within 14 days
HFMD (Hand- Place in a clean screw- capped Transport in ice to be
Stool/Rectal swab 5 to 10 ml if from onset of Tuesday Monday 10 days 4,000.00
foot-mouth container received at RITM
diarrheic symptoms
disease) within 72 hours

Place container in a
ziplock/leak-proof
plastic bags
Immediately
refrigerate at 2-8
degree Celsius while
awaiting transport
Molecular test for Within 7 to 10
HFMD (Hand- days after from Collect aseptically in a sterile Transport in ice to be
CSF 1 ml Tuesday Monday 10 days 4,000.00
foot-mouth onset of glass/plastic container received at RITM
disease) symptoms within 72 hours

Place container in a
ziplock/leak-proof
plastic bags
Immediately
refrigerate at 2-8
degree Celsius while
Molecular test for
· Use Dacron or rayon swabs with awaiting transport
HFMD (Hand- Within 5 days from
NPS/OPS 2 swabs plastic shafts Tuesday Monday 10 days 4,000.00
foot-mouth onset of fever
· Suspend in VTM/UTM Transport in ice to be
disease)
received at RITM
within 72 hours

Page 59 of 64
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LABORATORY SPECIMEN COLLECTION/ STORAGE DAY AND TAT FEE
QUANTITY COLLECTION TRANSPORT DAY
PROCEDURE TIME
Place container in a
ziplock/leak-proof
plastic bags

Immediately
refrigerate at 2-8
degree Celsius while
awaiting transport
Molecular test for
· Use Dacron or rayon swabs with
HFMD (Hand- With presence of Transport in ice to be
Vesicular swab 2 swabs plastic shafts Tuesday Monday 10 days 4,000.00
foot-mouth unraptured vesicle received at RITM
· Suspend in VTM/UTM
disease) within 72 hours

Place container in a
ziplock/leak-proof
plastic bags
Immediately
refrigerate at 2-8
degree Celsius while
awaiting transport
Genital swab
· Use Dacron or rayon swabs with
Molecular test for Endocervical swab Within 3 days from Transport in ice to be
2 swabs plastic shafts Tue/Thu Mon/Wed 7 days 4,000.00
HSV 1 & 2 Urethral swab onset of vesicles received at RITM
· Suspend in VTM/UTM
Vesicular swab within 72 hours

Place container in a
ziplock/leak-proof
plastic bags
Immediately
refrigerate at 2-8
degree Celsius while
awaiting transport
Within 7 to 10
Molecular test for Collect aseptically in a sterile Transport in ice to be
CSF 1 ml days from onset of Thursday Wednesday 10 days 4,000.00
HSV 1 & 2 glass/plastic container received at RITM
encephalitis
within 72 hours

Place container in a
ziplock/leak-proof
plastic bags

Page 60 of 64
SPECIAL LABORATORY TESTS: NON-HUMAN SAMPLE TESTING
AVAILABLE CUT OFF
VOLUME/ TIME OF HANDLING/ RUNNING
LABORATORY SPECIMEN COLLECTION/ STORAGE DAY AND TAT FEE
QUANTITY COLLECTION TRANSPORT DAY
PROCEDURE TIME
Mon-Fri A day
(except after a
holidays) batch test
Anti-Rabies -20°C if testing is (Batch
Antibody Detection At least 2 weeks Use serum separator gel/red top delayed. 2°C - 8°C if to Batch Testing: testing
Animal Serum At least 0.5 ml -- 5,000.00
after vaccination blood collection tube. Use gloves be tested immediately. 10th and 25th done
ELISA Transport in ice. of the month. every 10th
Release of and 25th
results on the day of the
next day. month)

Place the head inside

a leak proof plastic
bag, then place the 72
sealed plastic bag into working
a secondary container. hours
Rabies Virus To be transported to Mon-Fri upon
Antigen Detection Wear personal protective
laboratory within 6-8 (except receipt by No charge
equipment (gown, eye protection,
direct As soon as the hours after death with holidays), the until
Animal Head n/a double gloves, mask). Cut the --
animal died ice. If cannot be acceptance of Special further
FLUORESCENT head using a sharp knife. Place in
immediately brought specimen is 24 Pathogen notice
ANTIBODY TEST cold chain with ice.
to the laboratory, hours s
(dFAT)
place the specimen in Laborator
a freezer or ice chest y (VRD-
with frequent addition SPL)
of ice. Always
transport with ice.

Use serum separator

Mon-Thu
Use serum separator gel/ red top gel/ red top blood 3 working
Ebola Virus IgG Animal Serum 0.5 ml As required (except -- 700.00
blood collection tube. Use gloves. collection tube. Use days
ELISA holidays)
gloves.

Taxonomic Insect and other a single * Collection – clients will collect 1-5 210.00
identification of medically- specimen may the specimen submitted to the see collection / working
medically- important be accepted on -- department. Entomology staff Mon-Fri -- days for Per
storage procedures
important insects arthropods; can condition that shall prepare the specimen as specimen species
& other arthropods either be live or most anatomical appropriate to identification and that does

Page 61 of 64
causing diseases, preserved parts are following requirements of not need
allergy, and others specimen present. additional tests subsequent to special
However identification. preparatio
submission of 3 n prior to
or more * Storage – submitted samples identificat
individuals of the may be stored in small rigid ion
same species containers that protect from
may be more physical damage. They may 5-10
appropriate either be in 70% alcohol, dried or working
frozen depending on days for
requirements of additional tests specimen
subsequent to identification. to be
slide-
* Handling – unless samples will mounted
be subjected to further analysis prior to
with specific requirements, no identificat
special handling is required. ion

* Transport – for preserved or

dried specimens, samples should
be brought in the lab in proper
container as cited above.
However, refer to transport
requirements of “dangerous
goods” when alcohol or dry ice
are included

Page 62 of 64
SPECIAL LABORATORY TESTS: PUBLIC HEALTH CONCERN To be coordinated with the Surveillance and Response Unit of RITM
AVAILABLE CUT OFF
VOLUME/ TIME OF HANDLING/ RUNNING
LABORATORY SPECIMEN COLLECTION/ STORAGE DAY AND TAT
QUANTITY COLLECTION TRANSPORT DAY
PROCEDURE TIME

EBOLA VIRUS
Use serum separator
Mon-Thu
Ebola Virus IgG Use serum separator gel/ red top gel/ red top blood 3 working
Serum 0.5 ml As required (except --
ELISA blood collection tube. Use gloves. collection tube. Use days
holidays)
gloves.
Collect in 1 plastic EDTA (purple-
top) tube. In addition, also collect
either in 1 plastic red tube or in one Mon-Fri
Decontaminate tube
plastic yellow (serum separator) (regular testing
exterior with 0.5 % (v/v)
tube. If centrifugation is required, schedule);
Molecular test for sodium hypochlorite.
3-10 days after use a Saturday and
Ebolavirus Zaire Whole blood > 4 ml per tube Pack and transport as 10:00 AM 48 hours
onset of symptoms dedicated centrifuge with leak-proof Sunday (for
strain Category A infectious
rotor lid, preferably use within samples
substances (UN 2814)
biosafety cabinet, secure tube cover classified as
and at -20 °C
with sealing film, and do not urgent)
uncover tube once collection is
completed.

CORONAVIRUSES (MERS-CoV, SARS-CoV)

For symptomatics, For symptomatic:
Refrigerate prior to
collect both: collect on
≥500µl for NPS transport;
Lower respiratory presentation of
and/or OPS, Transport in ice within
tract: sputum in symptoms;
nasopharyngeal 48 hours after
sterile container, Collect using Dacron/Rayon-tip Mon-Fri
wash, collection;
Molecular test for endotracheal For asymptomatic swabs with plastic shaft and place (regular testing
nasopharyngeal
Middle East aspirate in sterile contacts: immediately in VTM. Wrap schedule);
aspirate, sputum, If the specimen is
Respiratory container, or within 14 days of specimen tubes in absorbent Saturday and
endotracheal expected to reach lab 10:00 AM 48 hours
Syndrome bronchoalveolar last documented material. Place in 50 ml conical Sunday (for
aspirate, beyond 72 hrs, store at -
Coronavirus lavage in sterile contact; to confirm tube, then inside ziplock bag. samples
bronchoalveolar 20C to -80C and ship on
(MERSCoV) container, lung tissue clearance of the Consult Specimen Collection classified as
lavage dry ice;
without fixative virus, sample Guidelines at RITM website urgent)
1-2g for unfixed
(post-mortem) in collection to
lung tissue Transport as Category B
VTM or sterile saline; be repeated until the
infectious substances
Upper respiratory results
(UN 3373)
tract: NPS and/or are negative on 2

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 AVAILABLE CUT OFF
VOLUME/ TIME OF HANDLING/ RUNNING
LABORATORY SPECIMEN COLLECTION/ STORAGE DAY AND TAT
QUANTITY COLLECTION TRANSPORT DAY
PROCEDURE TIME
OPS in VTM, sequential
nasopharyngeal samples.
aspirate in sterile
container

For asymptomatic
contact:
NPS and OPS in VTM
Lower respiratory
tract specimen (as
identified above), if
possible

INFLUENZA VIRUSES
Immediately
refrigerate at 2-8
degree Celsius while
Molecular test for Within 5 days from · Use Dacron or rayon swabs with
awaiting transport
Avian Influenza NPS/OPS 2 swabs onset of plastic shafts Thursday Wednesday 10 days 5,000.00
(H5, H7) symptoms · Suspend in VTM/UTM
Transport in ice to be
received at RITM
within 72 hours
Immediately
refrigerate at 2-8
Endotracheal degree Celsius while
Molecular test for Within 7 days from
Aspirate; Collect aseptically in a sterile awaiting transport
Avian Influenza 2 ml onset of Thursday Wednesday 10 days 5,000.00
Bronchoalveolar glass/plastic container
(H5, H7) symptoms
lavage Transport in ice to be
received at RITM
within 72 hours

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