GOVERNMENT OF JAMMU AND KASHMIR,
SERVICES SELECTION BOARD,
Zum Zum, Hotel Rambagh, Srinagar.
(www.jkssb.nic.in)
Syllabus for written test (Objective Type)
for the posts of Jr. Lab. Technician”
(HEALTH DEPARTMENT)”
Marks :-150
Time :- 2.30 Hours
ANATOMY THEORY 10 Marks
1. INTRODUCTION
Different Parts of the human body, Common Anatomical Terms,
Anatomical Positions and important planes.
Animal Cell.
Tissue of the body, classification and function.
Primary tissues of the body.
2. SKELETAL SYSTEM
Bones,Joints & Movements
Muscle & Monce.
3. GASTRO-INTESTINAL SYSTEM
Mouth and Pharynx.
Salivary Glands and Tonsils.
Oesophagus and Stomach.
Location of different organs in the Abdomen in situ.
Liver and Gall Bladder.
Spleen and Pancreas.
4. GENITO-URINARY SYSTEM
Kidney
Ureters, Bladder and Urethra.
Male Reproductive System.
Female Reproductive System.
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5. RESPIRATORY SYSTEM
Thoracic Cavity, Pleura and Lungs.
6. CARDIO-VASCULAR SYSTE
Heart Structure.
Arterial System.
Venous and Lymphatic System.
7. NERVOUS SYSTEM
Cerebrospinal fluid.
Brain, Spinal Cord and the Nerves.
8. LOCO-MOTOR SYSTEM
Parts of Upper Limb: - Bones Land marks and important vessels.
PHYSIOLOGY THEORY 15 Marks
1. BLOOD
Composition and General function of Blood.
Description of Blood Cells: - Normal Counts and function.
Anti-congulants. 03
2. CARDIO-VASCULAR SYSTEM 01
Functions of heart and blood vessels.
Circulation:- Systemic Circulation
Pulmonary Circulation.
3. RESPIRATORY SYSTEM 02
Name of the Structure involved in respiration and their function
External and Internal Respiration. How respiration and expiration are
brought about.
Transport of O2 and CO2 in the blood.
Definition of Respiratory Rate, Tidal Volume, Vital Capacity, Cyanosis,
Hypoxia.
4. EXCRETORY SYSTEM 01
Functions of Kidney.
Formation & Composition of Urine-normal and abnormal constituents.
5. SKIN
Functions of Skin. 01
6. DIGESTIVE SYSTEM 02
Composition and functions of Saliva. Mastication and deglutition.
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Functions of Stomach, Composition of Gastric Juice, Pancreatic Juice,
Bile and Succus entericus.
7. ENDOCRINE GLANDS 01
Definition, name and the hormones secreted by them.
Major action of each hormone.
8. REPROCUCTIVE SYSTEM 02
Male Genital System.
Female Genital System.
Names of Primary and Accessory Sex organs in male and female,
Secondary Sexual characters in male and female.
Functions of Ovary, formation of Ova, actions of Ovarian Hormones.
Functions of Testis – Spermatogenesis and actions of testosterone.
9. BLOOD GROUP, ABO and Rh, basis for classification, basis for
determination, importance and Blood Groups. 01
10. CEREBROSPINAL FLUID, Formation, composition and functions. 01
BIO-CHEMISTRY THEORY 10 Marks
1. Elementary knowledge of Inorganic Chemistry – Atomic Weight, Molecular
weight, Equivalent weight-Acids, basis and Salts-indicators-molar
solutions, Buffer Solution, Titration (Acid Base) Definition of Solutions.
Methods of expressing concentration: Dilution.
2. Elementary knowledge of Organic Chemistry-Organic Compounds.
Aliphatic and Aromatic. Alcohols, Aldehydes, Ketones, Amines, Esters,
Phenol, Acids Colloids etc.
3. Elementary of Analytical Chemistry-Instrumentation, Centrifuge Balances,
Colorimeter, Spetrophtometer, Flamephotometer Fluorimeter etc.
CLINICAL BIOCHEMISTRY THEORY 25 Marks
1. Aims and Scope Biochemistry.
2. CARBOHYDRATES – Importance, Definition, Classification, some
properties.
3. PROTEINS-Amino Acids, essential amino acids, peptides, denaturation of
proteins, Physiologically important proteins, functions of plasma proteins.
4. LIPIDS-Definition, classification, steroids, examples.
5. NUCLEICACIDS-DNAAND RNA, their importance.
6. HAEMOGLOBIN.
7. ENZYMESAND CO-ENZYMES-Elementary.
8. GASTRIC JUICE collection, Acidities.
9. CARBOHYDRATE-METABOLISM- elementary aspects, definition of
Glycolysis, Glycogenoysis Hormonal regulation of Blood Sugar, Diabetes-
Mellitus-Ketosis, Gcosuria, Renal Glycosuria, Pentosuria.
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10. METABOLISM OF LIPIDS – elementary aspects, Triglyeerides, Cholestrol.
Plasman Lipoprotiens-Ketone bodies and Ketonuria.
11. PROTIEN METABOLISM – Formation of Urea. Creatinine Proteinuria.
Edema, Transaminases.
12. WATER AND MINERAL METABOLISM – Dehydration, Calcium,
Phosphorus, Sodium, Potassium, Chloride, Iron, Lodine, their physiological
functions and disease state.
13. HARMONES – definition, functions of some important hormones.
14. Blood and cerebrospinal Fluid functions of Blood & CSF.
15. Urine Normal and abnormal tests.
MICROBIOLOGY AND PARASITOLOGY THEORY 30 Marks
1. Requirement and use of Common Laboratory Equipment
Incubator, Hot Air Oven. Autoclave. Water Bath. Anacrobic Jar. Vaccum
Pump. Media Pouring Chamber, Refrigerator. Centrifuge.
2. Microscope
Principal, Operation, Care and use of Microscope.
3. Sterilization and Disinfection
Classification and general principles of Sterilization. Physical. Chemical
and Mechanical Methods. Disposal of contaminated Media, Syringes,
Glassware, Apparatus.
4. Classification and Morphology of Bacteria
Brief Outline of :-
Structure of Cell, Capsule, Flagella and Spores.
Growth of Bacteria
Nutrition of Bacteria.
5. Staining of Bacteria
Simple, Grams, Ziehl-Neelsen, Albert. Spore Stain.
Composition and preparation of staining reagents.
6. Cultivation of Micro-Organisms-I (In detail)
Classification of Media, Composition of Laboratory culture Media and
Special Media.
7. Cultivation of Micro-Organisms-II (In detail)
8. Identification of Bacteria:
Cultural Characters, Bio-Chemical reactions and serotyping.
9. Normal flora of micro-organisms in the human body.
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10. Gram Positive and Gram Negative co---Staphylo----Penumococcus
Neissriae (In brief).
11. Gram Negative Bacilli:
Salmonella. Shigella. E. Coli. Klebsiella, Protein. Pseudomonas Vibro
cholera Haemophilus. (In Brief)
12. Gram Positive Bacilli:
Aerobic
a) Corynebacterium diphtheria. (In Brief).
b) Mycobacterium tubercoulosis and Mycobacterium leprae.
Anacrobic bacilli – Clostridia. (In Brief).
13. Antibiotic Sensitivity test – Principles and methods of determination of
sensitivity. Candida. Asperigillus. Dermatophytes. (In Brief).
14. HIV & AIDS:
Brief Account
15. Immunity, Antigens, Antibodies and antigen antibody reaction and their
applications in diagnosis of diseases.
16. Principles, Procedures and Diagnostic significance of agglutination.
Precipitation. Neutralisation and complement fixation reactions.
17. Collection and processing of Clinical materials like Sputum. Urine Swabs.
Stool. Blood, CSF and Aspirates.
CLINICAL PATHOLOGY AND HAEMATOLOGY 30 Marks
THEORY:
1. Introduction of Haemotology.
2. Collection of Blood
3. Antieoagulants.
4. Red Cell Count:
Haemocytometer
Methods
Caloculation.
5. White Cell Count. (Total Leucocyte Count):
Morphology of White Cells.
Normal Values.
Romanowsky Stains
Staining Precedures
Counting Methods.
6. Absolute Eosi Nophil Count.
7. Erytrocyte Sedimentaion Rate (ESR)
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Westergren’s Method.
Wintrobe’s Method.
Factors effecting ESR.
Importance and Limitations.
Normal Values.
8. Packed Cell Volume.
Macro and Micro Methods.
Normal Values.
9. Haemoglobin Estimation and its clinical importance.
10. Red Cell Indices
Calculations and Importance.
11. Retienlocyte Count:
Methods
Appearance
Normal Values.
12. Sickle Cell Preparation.
13. Osmotic Fragility Test:
Scorning Test.
Qualitative and Quantitative Test
Normal Values.
Factors allocating fragility.
Interpretation.
14. Peripheral Blood Film
15. Preparation of Bone Marrow Smears
16. Coagulation Tests.
Process of Coagulation.
Factors of Coagulation.
Tests of Coagulation.
a. Bleeding time.
b. Whole Blood Coagulation Time.
c. Clot Retraction Test.
d. Prothrombin Test.
e. Toorniquet test.
f. Platelet Count.
17. Urimanalysis.
Normal Constituent.
Physical Examination.
Chemical Examination.
Microscopic Examination.
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18. CSF Examination.
Normal and abnormal Cell Count.
19. Semen Analysis.
Physical Preterition.
Motility.
Morphology.
20. Coomb’s Test
HISTOTECHNOLOGY THEORY 14 Marks
1. Introduction.
2. Cell, Tissues and Their functions.
3. Examination Methods of Tissues and Cells.
4. Fixation of Tissue:
Classification of fixatives:
a) Simple fixatives and their properties.
b) Micro anatomical fixatives.
c) Cytological fixatives.
5. Tissue Processing:
Collection of Specimen.
Labeling and Fixation.
Dehydration.
Cleaning
Impregnation.
6. Section Cutting:
Microtomes and their knives.
Techniques of Section Cutting.
Mounting of Sections.
Frozen Section.
7. Staining.
Dyes and their properties.
Theory of Staining.
Staining Techniques with haemotoxlin and cosin.
Mounting of Sections.
Common Special Stains.
8. Decalification.
Fixation.
Decalification
Detection of end point.
Neutralization and processing.
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9. Exfoliative Cytology:
Types of Specimen and preservation.
Preparation and fixation of Smears.
Papanicolaou Staining Technique.
Sex Chromatin Staining.
10. Museum Technique.
Reception of Specimen.
Preparation of Fixation.
Restoration of Colour.
Preservation.
Presentation.
11. Autopsy Technique:
Assisting in Autopssy.
Preservation of Organs & Processing of Tissues.
12. Waste disposal and safety in Laboratory.
LABORATORY MANAGEMENT AND ETHICS 06 Marks
1. Role of the Laboratory in the Health Care Delivery System:
General
Human Health & Diseases.
a. Types of Diseases.
b. Process of Diagnosis
c. Laboratory at different levels.
d. Duties and responsibilities of Laboratory personnel.
2. Laboratory Service in the Health Care Delivery System in India.
Laboratory Service in India.
The Health Administration System in India.
a. At the National Level.
b. At the State Level.
c. At the District Level.
d. At the Village Level.
e. Voluntary health Organizations in India.
3. Laboratory Planning:
General Principals.
Laboratory Goals.
Operational Data.
a. Market Potential
b. Hospital/Laboratory relatives.
c. Competitions.
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d. Laboratory Trends.
e. Planning at different levels.
f. Guiding Principles for planning Hospital laboratory Services:
- Factors.
- Guiding Principles for Planning.
- Functional Criteria.
- Operational demand.
- Sections of a Hospital Laboratory.
- Common area.
- Design aspect.
- Space requirement.
g. Planning for a basic health Laboratory.
4. Laboratory Organization (Laboratory Management Techniques):
General Principles.
Components and functions of a laboratory.
Staffing the Laboratory.
Job Descriptions.
Job Specification
Work schedule
Personnel re-arrangement and work load assessment.
5. Care of Laboratory Glassware, Equipments and Instruments and Chemicals
etc.
General Principles.
Care and cleaning of glassware.
Making simple glass wares in Laboratory.
Care of equipments, instruments and apparatus etc.
Laboratory Chemicals, their proper use and care.
Labelling.
6. Specimen Handeling
General Principles.
Collection Techniques and containers for specimen.
Types of Specimens.
Specimens entry.
Specimens transfer and distribution and re-assignment.
Specimens disposal.
Specimens Preservation.
7. Laboratory Safety.
General Principles
Laboratory Hazards.
Safety Programmes.
First-Aid
8. Blood Bank. 10 Marks
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i) Introduction and Historical aspects.
ii) Human Blood Group Antigens, their inheritance and antibodies.
iii) ABO Blood Group System.
a) Sub-Group
b) Source of Antigens, types of antibodies.
iv) Rh. Blood Group System.
a) Nomenclature and types of Antigens.
b) Mode of inheritance.
c) Types of Antibodies.
V) Other Blood Group System.
Vi) Techniques of Grouping and Cross Matching.
Vii) Blood Collection.
a) Selection and screening of Donor.
b) Collection of Blood.
c) Various anticoagulants used.
d) Storage of Blood.
Viii) Blood Transfusion:
a) Procedures and Complications.
b) Blood Transfusion Reaction, Types, Investigation and Presentation of
Transfusion Reaction.
IX) Coomb’s Test.
X) Organization, Operation and Administration and Blood Bank.
Secretary,
J&K Services Selection Board,
Srinagar.
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