SAGE University, Indore

Institute of Biological Science Biotechnology

Code Title
B.Sc. I Sem.
BBTBCM01T BIOCHEMISTRY AND METABOLISM
L T/M P N C
Credit Score
3 1 - - 4
Prerequisites
BBTBCM01T; Biochemistry and Metabolism
(if any)
1. Understand the concept of Biochemistry regarding Biomolecules
Course Carbohydrates, proteins, lipids, Nucleic acids, Enzymes, Minerals
Objectives 2. Have knowledge of intermediary metabolism of the above & regulation of
individual metabolism
UNIT I: A historical prospective. Amino acids & Proteins: Structure & Function.
Structure and properties of Amino acids, Types of proteins and their classification,
Forces stabilizing protein structure and shape. Different Level of structural
organization of proteins, Protein Purification.Denaturation and renaturation of
proteins.Fibrous and globular proteins.
UNIT II: Carbohydrates: Structure, Function and properties of Monosaccharides,
Disaccharides and Polysaccharides. Homo & Hetero Polysaccharides,
Mucopolysaccharides, Bacterial cell wall polysaccharides, Glycoprotein’s and their
biological functions.
UNIT III: Enzymes: Nomenclature and classification of Enzymes, Holoenzyme,
apoenzyme, Cofactors, coenzyme, prosthetic groups, metalloenzymes, monomeric
&oligomeric enzymes, activation energy and transition state, enzyme activity,
specific activity, common features of active sites, enzyme specificity: types &
Course theories, Biocatalysts from extreme thermophilic and hyperthermophilicarchaea
Content
and bacteria. Role of: NAD+, NADP+, FMN/FAD, coenzymes A, Thiamine
pyrophosphate, Pyridoxalphosphate,lipoic-acid, Biotin vitamin B12,
Tetrahydrofolate and metallic ions.
UNIT IV: Carbohydrates Metabolism: Reactions, energetics and regulation.
Glycolysis: Fate of pyruvate under aerobic and anaerobic conditions. Pentose
phosphate pathway and its significance, Gluconeogenesis, Glycogenolysis and
glycogen synthesis. TCA cycle, Electron Transport Chain,
Oxidative phosphorylation. ß-oxidation of fatty acids.
UNIT V: Lipids: Structure and functions –Classification, nomenclature and
properties of fatty acids, essential fatty acids. Phospholipids, sphingolipids,
glycolipids, cerebrosides, gangliosides, Prostaglandins, Cholesterol. Nucleic acids:
Structure and functions: Physical & chemical properties of Nucleic acids,
Nucleosides & Nucleotides, purines &pyrimidines,. Biologically important
 SAGE University, Indore
nucleotides, Double helical model of DNA structure and forces responsible for A,
B & Z – DNA, denaturation and renaturation of DNA
 Berg, J. M., Tymoczko, J. L. and Stryer, L. (2006). Biochemistry.VI Edition.
W.H Freeman and Co.
 Buchanan, B., Gruissem, W. and Jones, R. (2000) Biochemistry and Molecular
Texts
Biology of Plants.American Society of Plant Biologists.
 Nelson, D.L., Cox, M.M. (2004) Lehninger Principles of Biochemistry, 4th
Edition, WH Freeman and Company, New York, USA.
 Hopkins, W.G. and Huner, P.A. (2008) Introduction to Plant Physiology. John
Wiley and Sons.
References  Salisbury, F.B. and Ross, C.W. (1991) Plant Physiology, Wadsworth Publishing
Co. Ltd.
On completion of this course a student should be able to understand
1. Be a competent Biochemist
2. Carry out & conduct various research problems both at basic and applied level.
3. Know the reactions of the major catabolic and anabolic pathways of
Course
carbohydrate, lipid, and amino acid metabolism.
Outcomes
4. Identify the five classes of polymeric biomolecules and their monomeric
building blocks.
5. Summarize what is currently known about the biochemical basis of DNA.

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 SAGE University, Indore
Institute of Biological Science Biotechnology
Code Title
B.Sc. I Sem.
BBTBCM01P BIOCHEMISTRY AND METABOLISM
L T/M P N C
Credit Score
- - 2 - 1
Prerequisites (if
BBTBCM01P; BIOCHEMISTRY AND METABOLISM
any)
Understand the concept of Biochemistry regarding Biomolecules Carbohydrates,
Course proteins, lipids, Nucleic acids, Enzymes, Minerals
Objectives Have knowledge of intermediary metabolism of the above & regulation of
individual metabolism
1. Study the effect of temperature and organic solvents on semi permeable
membrane.
2. Demonstration of dialysis.
3. Study of plasmolysis and de-plasmolysis.
4. Cell fractionation and determination of enzyme activity in organelles using
PRACTICALS:
sprouted seed or any other suitable source.
5. Study of structure of any Prokaryotic and Eukaryotic cell.
6. Microtomy: Fixation, block making, section cutting, double staining of animal
tissues like liver, oesophagus, stomach, pancreas, intestine, kidney, ovary, testes.
7. Cell division in onion root tip/ insect gonads.
8. Preparation of Nuclear, Mitochondrial & cytoplasmic fractions.
On completion of this course a student should be able to understand
1. Be a competent Biochemist
2. Carry out & conduct various research problems both at basic and applied level.
Course 3. Know the reactions of the major catabolic and anabolic pathways of
Outcomes carbohydrate, lipid, and amino acid metabolism.
4. Identify the five classes of polymeric biomolecules and their monomeric
building blocks.
5. Summarize what is currently known about the biochemical basis of DNA.
 SAGE University, Indore
Institute of Biological Science Biotechnology
Code Title
B.Sc. I Sem.
BBTCBY01T CELL BIOLOGY
L T/M P N C
Credit Score
3 1 - - 4
Prerequisites
BBTCBY01T; CELL BIOLOGY
(if any)
1. Identify and illustrate the components of subcellular infrastructure of a
Course eukaryotic or a prokaryotic cell.
Objectives 2. Describe the functions of individual cells in a given tissue of a multicellular
organism.
UNIT I: Introduction and classification of organisms by cell structure, cytosol,
compartmentalization of eukaryotic cells, cell fractionation. Cell Membrane and
Permeability: Chemical components of biological membranes, organization and
Fluid Mosaic Model, membrane as a dynamic entity, cell recognition and
membrane transport.
UNIT II: Membrane Vascular system, cytoskeleton and cell motility: Structure and
function of microtubules, Microfilaments, Intermediate filaments. Endoplasmic
reticulum: Structure, function including role in protein segregation. Golgi complex:
Structure, biogenesis and functions including role in protein secretion.
Course UNIT III: Lysosomes: Vacuoles and micro bodies: Structure and functions
Content Ribosomes: Structures and function including role in protein synthesis.
Mitochondria: Structure and function, Genomes, biogenesis. Chloroplasts:
Structure and function, genomes, biogenesis Nucleus: Structure and function,
chromosomes and their structure.
UNIT IV: Extracellular Matrix: Composition, molecules that mediate cell
adhesion, membrane receptors for extra cellular matrix, macromolecules, regulation
of receptor expression and function. Signal transduction..
UNIT V: Cell division in plant and animal cell ,cell cycle , mitosis ,meosis ,
apotosis , Cancer: Carcinogenesis, agents promoting carcinogenesis, characteristics
and molecular basis of cancer
 Karp, G. 2010. Cell and Molecular Biology: Concepts and Experiments. 6th
Edition. John Wiley & Sons.Inc.
Texts
 De Robertis, E.D.P. and De Robertis, E.M.F. 2006.Cell and Molecular Biology.
8th edition.Lippincott Williams and Wilkins, Philadelphia.
 Cooper, G.M. and Hausman, R.E. 2009. The Cell: A Molecular Approach. 5th
References edition. ASM Press& Sunderland, Washington, D.C.; Sinauer Associates, MA.
 Becker, W.M., Kleinsmith, L.J., Hardin. J. and Bertoni, G. P. 2009.The World
 SAGE University, Indore
of the Cell. 7th edition. Pearson Benjamin Cummings Publishing, San Francisco.
On completion of this course a student should be able to understand
1. Exhibit a knowledge base in genetics, cell and molecular biology, and anatomy
and physiology.
2. Demonstrate the knowledge of common and advanced laboratory practices in
cell and molecular biology.
3. Students will understand the structures and purposes of basic components of
Course prokaryotic and eukaryotic cells, especially macromolecules, membranes, and
organelles.
Outcomes
4. Students will apply their knowledge of cell biology to selected examples of
changes or losses in cell function. These can include responses to environmental
or physiological changes, or alterations of cell function brought about by
mutation.
5. Students will understand the cellular components underlying mitotic cell
division.

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 SAGE University, Indore
Institute of Biological Science Biotechnology
Code Title
B.Sc. I Sem.
BBTCBY01P CELL BIOLOGY
L T/M P N C
Credit Score
- - 2 - -
Prerequisites (if
BBTCBY01P; CELL BIOLOGY
any)
1. Identify and illustrate the components of subcellular infrastructure of a
Course eukaryotic or a prokaryotic cell.
Objectives 2. Describe the functions of individual cells in a given tissue of a multicellular
organism.
1. To study activity of any enzyme under optimum conditions.
2. To study the effect of pH, temperature on the activity of salivary amylase
enzyme.
3. Determination of - pH optima, temperature optima, Km value, Vmax value,
Effect of inhibitor (Inorganic phosphate) on the enzyme activity.
PRACTICALS:
4. Estimation of blood glucose by glucose oxidase method.
5. Principles of Colorimetry: (i) Verification of Beer's law, estimation of protein.
(ii) To study relation between absorbance and % transmission.
6. Preparation of buffers.
7. Separation of Amino acids by paper chromatography.
8. Qualitative tests for Carbohydrates, lipids and proteins
On completion of this course a student should be able to understand
1. Exhibit a knowledge base in genetics, cell and molecular biology, and anatomy
and physiology.
2. Demonstrate the knowledge of common and advanced laboratory practices in
cell and molecular biology.
3. Students will understand the structures and purposes of basic components of
Course prokaryotic and eukaryotic cells, especially macromolecules, membranes, and
Outcomes organelles.
4. Students will apply their knowledge of cell biology to selected examples of
changes or losses in cell function. These can include responses to environmental
or physiological changes, or alterations of cell function brought about by
mutation.
5. Students will understand the cellular components underlying mitotic cell
division.
 SAGE University, Indore

Institute of Biological Science Biotechnology

Code Title
B.Sc. II Sem.
BBTPPY01T PLANT ANATOMY AND PHYSIOLOGY
L T/M P N C
Credit Score
3 1 - - 4
Prerequisites
BBTPPY01T; PLANT ANATOMY AND PHYSIOLOGY
(if any)
1. The arrangement of tissue and cells types within the dermal, ground, and
vascular tissue systems in vascular plants.
Course
2. The characteristics of specialized cells and their components.
Objectives
3. The relationship between internal structure, physiology, and ecology.
4. Evolutionary history and taxonomic variation of vascular plant anatomy
UNIT I: Anatomy The shoot and root apical meristem and its histological
organization, simple & complex permanent tissues, primary structure of shoot &
root, secondary growth, growth rings, leaf anatomy (dorsi-ventral and isobilateral
leaf)
UNIT II: Plant water relations and micro & macro nutrients Plant water relations:
Importance of water to plant life, diffusion, osmosis, plasmolysis, imbibition,
guttation, transpiration, stomata & their mechanism of opening & closing. Micro &
macro nutrients: criteria for identification of essentiality of nutrients, roles and
deficiency systems of nutrients, mechanism of uptake of nutrients, mechanism of
Course food transport
Content UNIT III: Photosynthesis- Photosynthesis pigments, concept of two photo systems,
photphosphorylation, calvin cycle, CAM plants, photorespiration, compensation
point Nitrogen metabolism- inorganic & molecular nitrogen fixation, nitrate
reduction and ammonium assimilation in plants.
UNIT IV: Growth and development Growth and development: Definitions, phases
of growth, growth curve, growth hormones (auxins, gibberlins, cytokinins, abscisic
acid, ethylene)
UNIT V:Physiological role and mode of action, seed dormancy and seed
germination, concept of photoperiodism and vernalization Carbon and nitrogen
metabolism
 Dickinson, W.C. 2000 Integrative Plant Anatomy. Harcourt Academic Press,
USA.
 Esau, K. 1977 Anatomy of Seed Plants. Wiley Publishers.
Texts
 Fahn, A. 1974 Plant Anatomy. Pergmon Press, USA and UK.
 Hopkins, W.G. and Huner, P.A. 2008 Introduction to Plant Physiology. John
Wiley and Sons.
 SAGE University, Indore
 Mauseth, J.D. 1988 Plant Anatomy. The Benjammin/Cummings Publisher,
USA.
 Nelson, D.L., Cox, M.M. 2004 Lehninger Principles of Biochemistry, 4 th
edition, W.H. Freeman and Company, New York, USA.
 Salisbury, F.B. and Ross, C.W. 1991 Plant Physiology, Wadsworth Publishing
References
Co. Ltd.
 Taiz, L. and Zeiger, E. 2006 Plant Physiology, 4 th edition, Sinauer Associates
Inc .MA, USA
On completion of this course a student should be able to understand
1. Discuss the structural components of plant cell walls and membranes. Compare
and contrast the characteristics of plastid types.
2. List and describe the anatomy and ecological significance of epidermal and
Course secretory structures.
Outcomes
3. Compare, contrast, draw and describe the taxonomic and evolutionary variation
in xylem and phloem components.
4. Design and develop solutions.
5. To gain knowledge about plant diversity.

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 SAGE University, Indore
Institute of Biological Science Biotechnology
Code Title
B.Sc. II Sem.
BBTPPY01TP PLANT ANATOMY AND PHYSIOLOGY
L T/M P N C
Credit Score
- - 2 - 1
Prerequisites (if
BBTPPY01TP; PLANT ANATOMY AND PHYSIOLOGY
any)
1. The arrangement of tissue and cells types within the dermal, ground, and
vascular tissue systems in vascular plants.
Course
2. The characteristics of specialized cells and their components.
Objectives
3. The relationship between internal structure, physiology, and ecology.
4. Evolutionary history and taxonomic variation of vascular plant anatomy
1. Preparation of stained mounts of anatomy of monocot and dicot’s root, stem &
leaf
2. Demonstration of plasmolysis by Tradescantia leaf peel.
PRACTICALS: 3. Demonstration of opening & closing of stomata
4. Demonstration of guttation on leaf tips of grass and garden nasturtium.
5. Separation of photosynthetic pigments by paper chromatography.
6. Demonstration of aerobic respiration.
7. Preparation of root nodules from a leguminous plant.
On completion of this course a student should be able to understand
1. Discuss the structural components of plant cell walls and membranes. Compare
and contrast the characteristics of plastid types.
2. List and describe the anatomy and ecological significance of epidermal and
Course secretory structures.
Outcomes
3. Compare, contrast, draw and describe the taxonomic and evolutionary variation
in xylem and phloem components.
4. Design and develop solutions.
5. To gain knowledge about plant diversity.
 SAGE University, Indore
Institute of Biological Science Biotechnology
Code Title
B.Sc. II Sem.
BBTMPY01T MAMMALIAN PHYSIOLOGY
L T/M P N C
Credit Score
3 1 - - 4
Prerequisites
BBTMPY01T; MAMMALIAN PHYSIOLOGY
(if any)
To make student aware of the role of the cell in life and living systems, and
understands the inter-relationships between sub-cellular structures that contribute
Course
to its functioning as a unit. The diversity of life, and understands inter-relationships
Objectives
among organs and organ systems within an organism, and inter- relationships
between an organism and its environment
UNIT I: Digestion and Respiration, Digestion: Mechanism of digestion &
absorption of carbohydrates, Proteins, Lipids and nucleic acids. Composition of
bile, Saliva, Pancreatic, gastric and intestinal juice Respiration: Exchange of gases,
Transport of O2 and CO2, Oxygen dissociation curve, Chloride shift.
UNIT II: Circulation Composition of blood, Plasma proteins & their role, blood
cells, Haemopoisis, Mechanism of coagulation of blood. Mechanism of working of
heart: Cardiac output, cardiac cycle, Origin & conduction of heart beat.
UNIT III: Muscle physiology and osmoregulation Structure of cardiac, smooth &
Course skeletal muscle, threshold stimulus, All or None rule, single muscle twitch, muscle
Content tone, isotonic and isometric contraction, Physical, chemical & electrical events of
mechanism of muscle contraction. Excretion: modes of excretion, Ornithine cycle,
Mechanism of urine formation.
UNIT IV: Nervous and endocrine coordination Mechanism of generation &
propagation of nerve impulse, structure of synapse, synaptic conduction, saltatory
conduction, Neurotransmitters
UNIT V:Mechanism of action of hormones (insulin and steroids) Different
endocrine glands– Hypothalamus, pituitary, pineal, thymus, thyroid, parathyroid
and adrenals, hypo & hyper-secretions
 Guyton, A.C. & Hall, J.E. (2006). Textbook of Medical Physiology. XI Edition.
Hercourt Asia PTE Ltd. /W.B. Saunders Company.
Texts
 Tortora, G.J. & Grabowski, S. (2006). Principles of Anatomy & Physiology. XI
Edition. John wiley & sons,Inc.
 SAGE University, Indore
On completion of this course a student should be able to understand
1. Describe the general principles of how the human body operates and function
through the neural sensory, neuromuscular, and cardiovascular axis.
2. Have a general understand of how each of the major physiological systems
interact and integrate at the molecular and/or cellular level.
Course 3. Describe the anatomical structural components of each of the major
Outcomes physiological systems and how they relate to their function.
4. Design, perform experiments, analyze and interpret data for investigating
complex problems.
5. Emphasis is placed on the human body, so that students are able to understand
the basics of human physiology.

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 SAGE University, Indore
Institute of Biological Science Biotechnology
Code Title
B.Sc. II Sem.
BBTMPY01P MAMMALIAN PHYSIOLOGY
L T/M P N C
Credit Score
- - 2 - -
Prerequisites (if
BBTMPY01P; MAMMALIAN PHYSIOLOGY
any)
To make student aware of the role of the cell in life and living systems, and
understands the inter-relationships between sub-cellular structures that contribute
Course
to its functioning as a unit. The diversity of life, and understands inter-
Objectives
relationships among organs and organ systems within an organism, and inter-
relationships between an organism and its environment
1. Finding the coagulation time of blood

2. Determination of blood groups

3. Counting of mammalian RBCs

PRACTICALS:
4. Determination of TLC and DLC

5. Demonstration of action of an enzyme

6. Determination of Haemoglobin

On completion of this course a student should be able to understand

1. Describe the general principles of how the human body operates and function
through the neural sensory, neuromuscular, and cardiovascular axis.
2. Have a general understand of how each of the major physiological systems
interact and integrate at the molecular and/or cellular level.
Course 3. Describe the anatomical structural components of each of the major
Outcomes
physiological systems and how they relate to their function.
4. Design, perform experiments, analyze and interpret data for investigating
complex problems.
5. Emphasis is placed on the human body, so that students are able to understand
the basics of human physiology.
 SAGE University, Indore

Institute of Biological Science Biotechnology

Code Title
B.Sc. II Sem.
BBTBBH01T BIOETHICS, BIOSAFETY, AND HUMAN WELFARE
L T/M P N C
Credit Score
2 1 - - 3
Prerequisites
BBTBBH01T; BIOETHICS, BIOSAFETY, AND HUMAN WELFARE
(if any)
1. Introduce the basic notions of ethics
Course 2. Identify the socio-historical basis of the debate on animal experimentation
Objectives
3. Define levels of ethical questioning in animal experimentation.

UNIT I: Industry: protein engineering; enzyme and polysaccharide synthesis,

activity and secretion, alcohol and antibiotic formation.
UNIT II : Agriculture: N2 fixation: transfer of pest resistance genes to plants;
interaction between plants and microbes; qualitative improvement of livestock.
UNIT III: Environments: e.g. chlorinated and non-chlorinated organ pollutant
degradation; degradation of hydrocarbons and agricultural wastes, stress
Course management, development of biodegradable polymers such as PHB.
Content
UNIT IV: Forensic science: e.g. solving violent crimes such as murder and rape;
solving claims of paternity and theft etc. using various methods of DNA finger
printing.
UNIT V: Health: e.g. development of non-toxic therapeutic agents, recombinant
live vaccines, gene therapy, diagnostics, monoclonal in E.coli, human genome
project.
 Sateesh MK (2010) Bioethics and Biosafety, I. K. International Pvt Ltd
Texts  Sree Krishna V (2007) Bioethics and Biosafety in Biotechnology, New age
international publishers
On completion of this course a student should be able to understand
1. Apply the techniques for human welfare.
2. Able to explain the importance of Biosafety.
Course
Outcomes 3. To justify societal, health and legal issues.
4. To understand the importance of human welfare.
5. To improve and solve the environmental issues.
 SAGE University, Indore
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 SAGE University, Indore

Institute of Biological Science Biotechnology

Code Title
B.Sc. II Sem.
BBTBBH01P BIOETHICS, BIOSAFETY, AND HUMAN WELFARE
L T/M P N C
Credit Score
- - 2 - 1
Prerequisites
BBTBBH01P; BIOETHICS, BIOSAFETY, AND HUMAN WELFARE
(if any)
1. Introduce the basic notions of ethics
Course 2. Identify the socio-historical basis of the debate on animal experimentation
Objectives
3. Define levels of ethical questioning in animal experimentation.

(Wherever wet lab experiments are not possible the principles and concepts can be
demonstrated through any other material or medium including videos/virtual labs
etc.)
1. Perform of ethanolic fermentaion using Baker’s yeast
PRACTICALS 2. Study of a plant part infected with a microbe
3. To perform quantitative estimation of residual chlorine in water samples
4. Isolation and analysis of DNA from minimal available biological samples
5. Case studies on Bioethics (any two)
On completion of this course a student should be able to understand
1. Apply the techniques for human welfare.
2. Able to explain the importance of Biosafety.
Course 3. To justify societal, health and legal issues.
Outcomes
4. To understand the importance of human welfare.
5. To improve and solve the environmental issues.
 SAGE University, Indore
Institute of Biological Science Biotechnology
Code Title
B.Sc. II Sem.
BBTEBT01T ENVIRONMENTAL BIOTECHNOLOGY
L T/M P N C
Credit Score
2 1 - - 3
Prerequisites
BBTEBT01T/ BBTEBT01P; ENVIRONMENTAL BIOTECHNOLOGY
(if any)
1. To consolidate student’s knowledge in environmental microbiology and
molecular biotechnology with emphasis on methods for characterizing,
Course studying, monitoring, and improving microorganisms and microbial activity
Objectives during pollution control.
2. To provide a solid knowledge on selection, design and operation of bioprocess
for supporting water, air, and soil treatment.
UNIT I: Conventional fuels and their environmental impact – Firewood, Plant,
Animal, Water, Coal and Gas. Modern fuels and their environmental impact –
Methanogenic bacteria, Biogas, Microbial hydrogen Production, Conversion of
sugar to alcohol Gasohol
UNIT II: Bioremediation of soil & water contaminated with oil spills, heavy
metals and detergents. Degradation of lignin and cellulose using microbes. Phyto-
remediation. Degradation of pesticides and other toxic chemicals by micro-
organisms- degradation aromatic and chlorinates hydrocarbons and petroleum
products.
Course UNIT III: Treatment of municipal waste and Industrial effluents. Bio-fertilizers
Content Role of symbiotic and asymbiotic nitrogen fixing bacteria in the enrichment of
soil. Algal and fungal biofertilizers (VAM).
UNIT IV: Bioleaching, Enrichment of ores by microorganisms (Gold, Copper and
Uranium). Environmental significance of genetically modified microbes, plants and
animals.
UNIT V: Global Environmental Problems: Ozone depletion, UV-B, green -house
effect and acid rain, their impact and biotechnological approaches for management.
Environmental Pollution: types of pollution, Methods for the measurement of
pollution; Methodology of environmental management - the problem solving
approach, its limitations.
 Environmental Science, S.C. Santra
 Environmental Biotechnology, Pradipta Kumar Mohapatra
Texts
 Environmental Biotechnology – Concepts and Applications, Hans-Joachim
Jordening and Jesef Winter
 SAGE University, Indore
 Waste Water Engineering, Metcalf and Eddy, Tata McGraw hill
 Agricultural Biotechnology, S.S. Purohit
 Environmental Microbiology : Methods and Protocols, Alicia L. Ragout De
Spencer, John F.T. Spencer
References  Introduction to Environmental Biotechnology, Milton Wainwright
 Principles of Environmental Engineering, Gilbert Masters
 Wastewater Engineering – Metcalf & Eddy.
On completion of this course a student should be able to understand
1. Identify the ever-increasing complexity and interrelated nature of current
environmental problems as well as the advantages of biotechnological treatment
of liquid, solid and gaseous waste
2. Identify fundamental scientific and engineering principles of microbiology of
Course environmental engineering systems, different microbiological classifications,
Outcomes
microbial systematics, microbial ecology, microbial metabolism,
natural/advanced environmental biotechnologies.
3. Solutions on environment and societal context.
4. To identify student’s knowledge in environmental microbiology.
5. Identify cost effective strategies through Bioremediation.

PO1 PO2 PO3 PO4 PO5 PO6 PO7 PO8 PO9 PO10 PO11 PO12

CO1 Y

CO2 Y

CO3 Y

CO4 Y

CO5 Y
 SAGE University, Indore
Institute of Biological Science Biotechnology
Code Title
B.Sc. II Sem.
BBTEBT01P ENVIRONMENTAL BIOTECHNOLOGY
L T/M P N C
Credit Score
- - 2 - 1
Prerequisites
BBTEBT01P; ENVIRONMENTAL BIOTECHNOLOGY
(if any)
1. To consolidate student’s knowledge in environmental microbiology and
molecular biotechnology with emphasis on methods for characterizing,
Course studying, monitoring, and improving microorganisms and microbial activity
Objectives during pollution control.
2. To provide a solid knowledge on selection, design and operation of bioprocess
for supporting water, air, and soil treatment.
1. Calculation of Total Dissolved Solids (TDS) of water sample.
2. Calculation of BOD of water sample.
PRACTICALS
3. Calculation of COD of water sample.
4. Bacterial Examination of Water by MPN Method.
On completion of this course a student should be able to understand
1. Identify the ever-increasing complexity and interrelated nature of current
environmental problems as well as the advantages of biotechnological treatment
of liquid, solid and gaseous waste
2. Identify fundamental scientific and engineering principles of microbiology of
Course environmental engineering systems, different microbiological classifications,
Outcomes
microbial systematics, microbial ecology, microbial metabolism,
natural/advanced environmental biotechnologies.
3. Solutions on environment and societal context.
4. To identify student’s knowledge in environmental microbiology.
5. Identify cost effective strategies through Bioremediation.
 SAGE University, Indore
Institute of Biological Science Biotechnology
Code Title
B.Sc. III Sem.
BBTGEN01T GENETICS
L T/M P N C
Credit Score
3 1 - - 4
Prerequisites
BBTGEN01T; GENETICS
(if any)
Be able to articulate connections between multiple levels of genetic organization:
Course molecular mechanisms, the expression of DNA at the individual level, and the
Objectives transmission of that DNA across generations at the individual and population
levels.
Unit I: Introduction: Historical developments in the field of genetics. Organisms
suitable for genetic experimentation and their genetic significance. Cell Cycle:
Mitosis and Meiosis: Control points in cell-cycle progression in yeast. Role of
meiosis in life cycles of organisms.
Unit II: Mendelian genetics: Mendel’s experimental design, monohybrid, di-hybrid
and tri hybrid crosses, Law of segregation & Principle of independent assortment.
Verification of segregates by test and back crosses, Chromosomal theory of
inheritance, Allelic interactions: Concept of dominance, recessiveness, incomplete
dominance, co-dominance, semi-dominance, pleiotropy, multiple allele, pseudo-
allele, essential and lethal genes, penetrance and expressivity. Non allelic
interactions: Interaction producing new phenotype complementary genes, epistasis
(dominant & recessive), duplicate genes and inhibitory genes.
Course Unit III: Genetic code, gene function. Chromosome and gene mutations:
Content Definition and types of mutations, causes of mutations, Ames test for mutagenic
agents, screening procedures for isolation of mutants and uses of mutants,
variations in chromosomes structure - deletion, duplication, inversion and
translocation (reciprocal and Robertsonian), position effects of gene expression,
chromosomal aberrations in human beings, abonormalities– Aneuploidy and
Euploidy.
Unit IV: Sex determination and sex linkage: Mechanisms of sex determination,
Environmental factors and sex determination, sex differentiation, Barr bodies,
dosage compensation, genetic balance theory, Fragile-X-syndrome and
chromosome, sex influenced dominance, sex limited gene expression, sex linked
inheritance.
Unit V: Genetic linkage, crossing over and chromosome mapping: Linkage and
Recombination of genes in a chromosome crossing over, Cytological basis of
 SAGE University, Indore
crossing over, Molecular mechanism of crossing over, Crossing over at four strand
stage, Multiple crossing overs, Genetic mapping. Extra chromosomal inheritance:
Rules of extra nuclear inheritance, maternal effects, maternal inheritance,
cytoplasmic inheritance, organelle heredity, genomic imprinting. Evolution and
population genetics: In breeding and out breeding, Hardy Weinberg law
(prediction, derivation), allelic and genotype frequencies, changes in allelic
frequencies, systems of mating, evolutionary genetics, natural selection.
 Principle of Genetics by Robert H. Tamarin, Tata-McGraw Hill, Seventh Edition
(2002).
 Genetics, Princilpes and Analysis by Daniel Hart and
 E.W. Jones. 4th Edition 1998; Jones and Bartlett Publication. The Science of
Texts
Genetics by Atherly, A.G.; Girton, J.R. nd MC Donald, JF. (1999). Sounders
College Publication / Harcourt Brace.
 Genetics by M.W. Strickbergar. McMillan Publication, New York.
 A History of Genetics by Sturtevant, A.H. (1965). Harper And Row New York
 Gregor Mendel: The First Geneticist by Orel V.(1996) Oxford University Press,
New York.
 A first course in Probability by Ross S. (1994); 4th Edition, McMillan, New York.
 Theory and problems of Genetics –W.D.Stansfield (Schaum’sOutline Series).
References
McGrawHill 2002.
 History of Genetics by Stubbe. H. (1972), Harper andRow New York.
 Fundamentals of Biostatistics by Khan &Khanum (2004), II Revised Edition,
Ukaaz Publication
On completion of this course a student should be able to understand
1. An understanding of the central theories and methodologies that define the
field of genetics and its various subdisciplines (traditional, molecular, and
population genetics) and the ability to use the vocabulary that embodies this
knowledge;
2. An understanding that science is a continual process of investigation and
interpretation and that scientific knowledge progresses via the support and
rejection of competing hypotheses, collective decisions that are based on
empirical evidence and logical interpretation using inductive and deductive
Course reasoning;
Outcomes 3. The ability to develop a scientifically informed position on some of the
bioethical and social issues related to the practice and application of genetics
research
4. And demonstration of enhanced critical inquiry skills through writing.
Specifically, students should view writing as a tool to explore and express ideas,
develop the ability to synthesize and critically evaluate information from
multiple sources and viewpoints, and apply such information to the construction
of an argument.
5. 5. Knowledge in genetics and understanding of related norms and ethics.
 SAGE University, Indore
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 SAGE University, Indore
Institute of Biological Science Biotechnology
Code Title
B.Sc. III Sem.
BBTGEN01P GENETICS
L T/M P N C
Credit Score
- - 2 - 1
Prerequisites
BBTGEN01P; GENETICS
(if any)
Be able to articulate connections between multiple levels of genetic organization:
Course molecular mechanisms, the expression of DNA at the individual level, and the
Objectives transmission of that DNA across generations at the individual and population
levels.
1. Permanent and temporary mount of mitosis.
2. Permanent and temporary mount of meiosis.
3. Mendelian deviations in dihybrid crosses
4. Karyotyping with the help of photographs
5. Study of floral structure of:
a) Pea / Crotolaria
PRACTICALS b) Maize
6. Temporary squash preparation of
a) Onion flower buds
7. Blood typing
8. Genetic problems
a) Multiple alleles
b) Gene interaction
On completion of this course a student should be able to understand
1. An understanding of the central theories and methodologies that define the field
of genetics and its various subdisciplines (traditional, molecular, and population
genetics) and the ability to use the vocabulary that embodies this knowledge;
Course 2. An understanding that science is a continual process of investigation and
Outcomes interpretation and that scientific knowledge progresses via the support and
rejection of competing hypotheses, collective decisions that are based on
empirical evidence and logical interpretation using inductive and deductive
reasoning;
3. The ability to develop a scientifically informed position on some of the
 SAGE University, Indore
bioethical and social issues related to the practice and application of genetics
research
4. And demonstration of enhanced critical inquiry skills through writing.
Specifically, students should view writing as a tool to explore and express ideas,
develop the ability to synthesize and critically evaluate information from
multiple sources and viewpoints, and apply such information to the construction
of an argument.
5. Knowledge in genetics and understanding of related norms and ethics.
 SAGE University, Indore
Institute of Biological Science Biotechnology
Code Title
B.Sc. III Sem.
BBTGEM01T GENERAL MICROBIOLOGY
L T/M P N C
Credit Score
3 1 - - 4
Prerequisites
BBTGEM01T/ BBTGEM01P; GENERAL MICROBIOLOGY
(if any)
In view of the increasing demand for training manpower in the area of
Microbiology, Genetic Engineering and Biotechnology, this course should be broad
Course
based and should be able to give a good insight into modern biology and important
Objectives
component of hands-on training to the students. Thus by nature it will be an
interdisciplinary course.
Unit I: Fundamentals, History and Evolution of Microbiology. Classification of
microorganisms: Microbial taxonomy, criteria used including molecular
approaches, Microbial phylogeny and current classification of bacteria.
Microbial Diversity: Distribution and characterization Prokaryotic and Eukaryotic
cells, Morphology and cell structure of major groups of microorganisms eg.
Bacteria, Algae, Fungi, Protozoa and Unique features of viruses.
Unit II: Cultivation and Maintenance of microorganisms: Nutritional categories of
micro-organisms, methods of isolation, Purification and preservation.
Microbial growth: Growth curve, Generation time, synchronous batch and
Course continuous culture, measurement of growth and factors affecting growth of
Content bacteria.
Unit III: Microbial Metabolism: Metabolic pathways, amphi-catabolic and
biosynthetic pathways Bacterial Reproduction: Transformation, Transduction and
Conjugation. Endospores and sporulation in bacteria.
Unit IV: Control of Microorganisms: By physical, chemical and chemotherapeutic
Agents Water Microbiology: Bacterial pollutants of water, coliforms and non
coliforms. Sewage composition and its disposal.
Unit V: Food Microbiology: Important microorganism in food Microbiology:
Moulds, Yeasts, bacteria. jor food born infections and intoxications, Preservation of
various types of foods. Fermented Foods.
 Microbiology, Authors- Pelczar, Chan and Kreig.
 Microbiology- an Introduction- (8th Edn), Authors- Tortora, G.J., Funke, B.R.,
Case, C.L.
Texts
 General Microbiology, Authors- Stainer, Ingharam, Wheelis and Painter.
 General Microbiology, Authors- Stainer RY. Ingharam JL. Wheelis ML. Painter
PR
 SAGE University, Indore
 Biology of Microorganisms, Authors- Brock and Madigan.
 Fundamental Principles of Bacteriology, Author- A.J. Salle.
References
 Introduction to Microbiology, Authors- Ingraham and Ingraham.
 Microbiology Laboratory Manual: Cappuccino and Sherman
On completion of this course a student should be able to understand
1. Comparative characteristics of microbial organisms
Course 2. General bacteriology and microbial techniques
Outcomes 3. Common bacterial, fungal, and viral diseases
4. Experimentation in clinical scenarios.
5. Undertake any responsibility as an individual.

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 SAGE University, Indore
Institute of Biological Science Biotechnology
Code Title
B.Sc. III Sem.
BBTGEM01P GENERAL MICROBIOLOGY
L T/M P N C
Credit Score
- - 2 - 1
Prerequisites
BBTGEM01P; GENERAL MICROBIOLOGY
(if any)
In view of the increasing demand for training manpower in the area of
Microbiology, Genetic Engineering and Biotechnology, this course should be broad
Course
based and should be able to give a good insight into modern biology and important
Objectives
component of hands-on training to the students. Thus by nature it will be an
interdisciplinary course.
1. Microscopy– Description and operation of compound microscope, use of oil
immersion objective.
2. Staining- Simple and differential staining of bacteria.
3. Sterilization- Operation of autoclave, hot air oven, membrane filtration
(demonstration only), culture room fumigation using formalin, surface
sterilization of phenol.
4. Culture media preparation- Nutrient broth, nutrient agar slant, potato dextrose
PRACTICALS agar.
5. Culture techniques-Streak plate, pour plate and spread plate (stab culture only
6. demonstration)
7. Isolation of pure culture by streak plate method.
8. Viable count of bacteria by serial dilution and pour plating.
9. Methylene blue reduction tests of milk Samples.
10. Antibiotic sensitivity tests by cup plate/paper disc method
11. Bacteriological examination of curd
On completion of this course a student should be able to understand
1. Comparative characteristics of microbial organisms
Course 2. General bacteriology and microbial techniques
Outcomes 3. Common bacterial, fungal, and viral diseases
4. Experimentation in clinical scenarios.
5. Undertake any responsibility as an individual.
 SAGE University, Indore

Institute of Biological Science Biotechnology

Code Title
B.Sc. III Sem.
BBTMOD01T MOLECULAR DIAGNOSTICS
L T/M P N C
Credit Score
2 1 - - 3
Prerequisites
BBTMOD01T/ BBTMOD01P; MOLECULAR DIAGNOSTICS
(if any)
Define terms related to molecular diagnostics. Identify appropriate specimen
Course collection and handling measures for molecular diagnostics. Classify the various
Objectives molecular methodologies. Outline examples of procedures for each molecular
diagnostic classification.
Unit I: Comparison of enzymes available for enzyme immunoassays, conjugation
of enzymes. Solid phases used in enzyme immunoassays. Homogeneous and
heterogeneous enzyme immunoassays. Enzyme immunoassays after immuno
blotting. Enzyme immuno histochemical techniques. Use of polyclonal or
monoclonal antibodies in enzymes immuno assays.
Applications of enzyme immunoassays in diagnostic microbiology.
Unit II: Molecular methods in clinical microbiology:
Applications of PCR, RFLP, Nuclear hybridization methods, Single nucleotide
polymorphism and plasmid finger printing in clinical microbiology
Course Unit III: Laboratory tests in chemotherapy:
Content Susceptibility tests: Micro-dilution and macro-dilution broth procedures.
Susceptibility tests: Diffusion test procedures. Susceptibility tests: Tests for
bactericidal activity. Automated procedures for antimicrobial susceptibility tests.
Unit IV: Automation in microbial diagnosis, rapid diagnostic approach including
technical purification and standardization of antigen and specific antibodies.
Concepts and methods in idiotypes. Antiidiotypes and molecular mimicry and
receptors. Epitope design and applications.
Immunodiagnostic tests. Immuno florescence. Radioimmunoassay.
Unit V: GC, HPLC, Electron microscopy, flowcytometry and cell sorting.
Transgenic animals.
 Molecular biology of the cell. Bruce Alberts, 6th Edition
 Principles of tissue engineering. Robert Lanza. Elsevier Publications.
 Introduction to Tissue engineering, applications and challenges. Ravi Birla. Wiley
Texts
 Publications.
 Molecular Cell Biology: Darnell J, Lodish H and Baltimore D
 Cell and Molecular Biology: De Robertis EDP and De Robertis EMF
 SAGE University, Indore
 Molecular Biology of the cell by Alberts et al., Garland Press
 Genes IX, by Lewin B, Pearson India
References
 Cell and Molecular Biology by De Robertis and De Robertis, Lipincott &Wilkins
 Genome III by Brown TA, Garland Press
On completion of this course a student should be able to understand
1. To understand knowledge of cellular structure and function, especially DNA
and RNA, to molecular diagnostic procedures.
2. Gain a thorough working knowledge of nucleic acid extraction, resolution and
detection.
Course 3. Gain a solid foundation in the most commonly utilized molecular diagnostic
Outcomes testing protocols.
4. Apply the knowledge of molecular testing to the most commonly performed
applications in the clinical laboratory such asanalysis and characterization of
nucleic acids and proteins, nucleic acid amplification and DNA sequencing.
5. Develop oral and written communication skills.

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 SAGE University, Indore
Institute of Biological Science Biotechnology
Code Title
B.Sc. III Sem.
BBTMOD01P MOLECULAR DIAGNOSTICS
L T/M P N C
Credit Score
- - 2 - 1
Prerequisites
BBTMOD01P; MOLECULAR DIAGNOSTICS
(if any)
Define terms related to molecular diagnostics. Identify appropriate specimen
Course collection and handling measures for molecular diagnostics. Classify the various
Objectives molecular methodologies. Outline examples of procedures for each molecular
diagnostic classification.
(Wherever wet lab experiments are not possible the principles and concepts can be
demonstrated through any other material or medium including videos/virtual labs
etc.)
1. Perform/demonstrate RFLP and its analysis
2. Kirby-Bauyer method (disc-diffusion method) to study antibiotic sensitivity of a

PRACTICALS bacterial
3. culture
4. A kit-basd detection of a microbial infection (Widal test)
5. Study of Electron micrographs (any four).
6. Perform any one immuno diagnostic test ( Typhoid, Malaria, Dengue)
On completion of this course a student should be able to understand
1. To understand knowledge of cellular structure and function, especially DNA
and RNA, to molecular diagnostic procedures.
2. Gain a thorough working knowledge of nucleic acid extraction, resolution and
detection.
Course 3. Gain a solid foundation in the most commonly utilized molecular diagnostic
Outcomes testing protocols.
4. Apply the knowledge of molecular testing to the most commonly performed
applications in the clinical laboratory such asanalysis and characterization of
nucleic acids and proteins, nucleic acid amplification and DNA sequencing.
5. Develop oral and written communication skills.
 SAGE University, Indore

Institute of Biological Science Biotechnology

Code Title
B.Sc. III Sem. BBTDEB01T/
DEVELOPMENTAL BIOLOGY
BBTDEB01P
L T/M P N C
Credit Score
2 1 - - 3
Prerequisites
BBTDEB01T; DEVELOPMENTAL BIOLOGY
(if any)
This course will provide upper division undergraduate students with an
Course understanding of the fundamental concepts, principles, and mechanisms of animal
Objectives development, as well as a familiarity with basic experimental methods and
laboratory models used in developmental biology research
Unit I: Definition, scope & historical perspective of development Biology,
Gametogenesis – Spermatogenesis, Oogenesis Fertilization - Definition,
mechanism, types of fertilization. Different types of eggs on the basis of yolk.
Unit II: Cleavage: Definition, types, patterns & mechanism Blastulation: Process,
types & mechanism Gastrulation: Morphogenetic movements– epiboly, emboly,
extension, invagination, convergence, de-lamination. Formation & differentiation
of primary germ layers, Fate Maps in early embryos.
Unit III: Differentiation: Cell commitment and determination- the epigenetic
Course landscape: a model of determination and differentiation, control of differentiation at
Content
the level of genome, transcription and post-translation level Concept of embryonic
induction: Primary, secondary & tertiary embryonic induction, Neural induction
and induction of vertebrate lens.
Unit IV: Neurulation, notogenesis, development of vertebrate eye. Fate of different
primary germ layers Development of behaviour: constancy & plasticity, Extra
embryonic membranes, placenta in Mammals.
Unit V: Medical implications of developmental biology - genetic errors/
teratogenesis/ stem cell therapy etc
 Scott F. Gilbert, Developmental Biology, Eighth Edition. Sinauer Associates,
2006
Texts
 Mary S. Tyler. Developmental Biology: A Guide for Experimental Study, 3rd ed.
(CD) Sinauer Associates, Inc. 2001
 Essential Developmental Biology by Jonathan Slack
References  Developmental Biology, Werner A Muller (link is external)
 Principles of Development - Lewis Wolpert (link is external)
 SAGE University, Indore
On completion of this course a student should be able to understand
1. Demonstrate a basic understanding of developmental terms and mechanisms.
2. Utilize laboratory techniques to design and carry-out experimental studies.
Course 3. Convey and discuss experimental results via written assignments.
Outcomes
4. Basic understanding of developmental stages of various model organisms.
5. Applying the knowledge of developmental biology in various researches related
to it.

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 SAGE University, Indore

Institute of Biological Science Biotechnology

Code Title
B.Sc. III Sem.
BBTDEB01P DEVELOPMENTAL BIOLOGY
L T/M P N C
Credit Score
- - 2 - -
Prerequisites
BBTDEB01P; DEVELOPMENTAL BIOLOGY
(if any)
This course will provide upper division undergraduate students with an
Course understanding of the fundamental concepts, principles, and mechanisms of animal
Objectives development, as well as a familiarity with basic experimental methods and
laboratory models used in developmental biology research
1. Identification of developmental stages of chick and frog embryo using
permanent mounts
2. Preparation of a temporary stained mount of chick embryo

PRACTICALS 3. Study of developmental stages of Anopheles.

4. Study of the developmental stages of Drosophila from stock culture/
photographs..
5. Study of different types of placenta.
On completion of this course a student should be able to understand
1. Demonstrate a basic understanding of developmental terms and mechanisms.
2. Utilize laboratory techniques to design and carry-out experimental studies.
Course 3. Convey and discuss experimental results via written assignments.
Outcomes
4. Basic understanding of developmental stages of various model organisms.
5. Applying the knowledge of developmental biology in various researches related
to it.
 SAGE University, Indore
Institute of Biological Science Biotechnology
Code Title
B.Sc. IV Sem.
BBTMOB01T MOLECULAR BIOLOGY
L T/M P N C
Credit Score
3 1 2 - 4/1
Prerequisites
BBTMOB01T; MOLECULAR BIOLOGY
(if any)
In view of the increasing demand for training manpower in the area of Molecular
Biology, Genetic, Medicine and Biotechnology, this course should be broad based
Course and should be able to give a good insight into modern biology and important
Objectives component of hands-on training to the students. Thus by nature it will be an
interdisciplinary course

Unit I: DNA as genetic material, Structure of DNA, Types of DNA, Replication of

DNA in prokaryotes and eukaryotes: Semiconservative nature of DNA replication,
Bi-directional replication, DNA polymerases, The replication complex: Pre-
primming proteins, primosome, replisome, Rolling circle replication, Unique
aspects of eukaryotic chromosome replication, Fidelity of replication.
Unit II: RNA structure and types of RNA, Transcription in prokaryotes:
Prokaryotic RNA polymerase, role of sigma factor, promoter, Initiation, elongation
and termination of RNA chains.
Unit III: Transcription in eukaryotes: Eukaryotic RNA polymerases, transcription
factors, promoters, enhancers, mechanism of transcription initiation, promoter
Course clearance and elongation RNA splicing and processing: processing of pre-mRNA:
Content 5’ cap formation, polyadenylation, splicing, rRNA and tRNA splicing.
Unit IV: Regulation of gene expression in prokaryotes: Operon concept (inducible
and repressible system), Genetic code and its characteristics, Prokaryotic and
eukaryotic translation: ribosome structure and assembly, Charging of tRNA,
aminoacyl tRNA synthetases, Mechanism of initiation, elongation and termination
of polypeptides, Fidelity of translation, Inhibitors of translation, Posttranslational
modifications of proteins.
Unit V: DNA damage and repair: causes and types of DNA damage, mechanism of
DNA repair: Photoreactivation, base excision repair, nucleotide excision repair,
mismatch repair, translesion synthesis, recombinational repair, nonhomologous end
joining. Homologous recombination: models and mechanism
 SAGE University, Indore
 T A Brown, various books. His books are well written; just check that the content
and level are what you want. Introductory books include Gene Cloning and DNA
Analysis: An Introduction (2001); Genomes 3 (2006).
Texts  R F Weaver, Molecular Biology, 2/e, 2002, WCB/McGraw-Hill.
 D P Clark & L D Russell, Molecular Biology made simple and fun, 2/e. Cache
River Press, 2000
 H Lodish et al, Molecular Cell Biology, 8/e, Freeman, 2016.
 B Alberts et al, Molecular Biology of the Cell. 6/e, 2014. ISBN 978-0815344322.
The Problems Book (Study guide) by Wilson & Hunt.
References
 G M Cooper & R E Hausman, The Cell - A Molecular Approach. 7/e, 2016.

On completion of this course a student should be able to understand

1. The structure and function of the eukaryotic cell and its organelles.
2. The genetic information flow in the eukaryotic cell; including nucleic acid
structures, the definition of a gene, the organisation of the genome, the
replication, the formation of RNA (transcription), the processing of pre mRNA
Course and the protein synthesis (translation).
Outcomes
3. The process of regulation of gene.
4. The process of communication of cells and the central intracellular signal
transduction pathway.
5. Experimentation in clinical scenarios.

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 SAGE University, Indore
Institute of Biological Science Biotechnology
Code Title
B.Sc. IV Sem.
BBTMOB01P MOLECULAR BIOLOGY
L T/M P N C
Credit Score
2 -
Prerequisites
BBTMOB01P; MOLECULAR BIOLOGY
(if any)
In view of the increasing demand for training manpower in the area of Molecular
Biology, Genetic, Medicine and Biotechnology, this course should be broad based
Course and should be able to give a good insight into modern biology and important
Objectives component of hands-on training to the students. Thus by nature it will be an
interdisciplinary course

1. Preparation of solutions for Molecular Biology experiments.

2. Isolation of chromosomal DNA from bacterial cells.
3. Isolation of Plasmid DNA by alkaline lysis method
PRACTICALS
4. Agarose gel electrophoresis of genomic DNA & plasmid DNA
5. Preparation of restriction enzyme digests of DNA samples
6. Demonstration of AMES test or reverse mutation for carcinogenicity
On completion of this course a student should be able to understand
1. The structure and function of the eukaryotic cell and its organelles.
2. The genetic information flow in the eukaryotic cell; including nucleic acid
structures, the definition of a gene, the organisation of the genome, the
replication, the formation of RNA (transcription), the processing of pre mRNA
Course and the protein synthesis (translation).
Outcomes
3. The process of regulation of gene.
4. The process of communication of cells and the central intracellular signal
transduction pathway.
5. Experimentation in clinical scenarios.
 SAGE University, Indore
Institute of Biological Science Biotechnology
Code Title
B.Sc. IV Sem.
BBTIMY01T IMMUNOLOGY
L T/M P N C
Credit Score
3 1 - - 4
Prerequisites
BBTIMY01T; IMMUNOLOGY
(if any)
The purpose of the Immunology course is to provide a basic knowledge of the
immune response and its involvement in health and disease. An effort has been
Course made to increase clinical relevance and problem-solving skills through an essay
Objectives
assignment and faculty presented clinical correlations, and a team based learning
exercise.
Unit I: Immune Response - An overview, components of mammalian immune
system, molecular structure of Immuno-globulins or Antibodies, Humoral &
Cellular immune responses, Tlymphocytes & immune response (cytotoxic T-cell,
helper T-cell, suppressor T-cells), T-cell receptors, genome rearrangements during
B-lymphocyte differentiation, Antibody affinity maturation class switching,
assembly of T-cell receptor genes by somatic recombination.
Unit II: Regulation of immunoglobulin gene expression – clonal selection theory,
allotypes & idiotypes, allelic exclusion, immunologic memory, heavy chain gene
Course transcription, genetic basis of antibody diversity, hypotheses (germ line & somatic
Content mutation), antibody diversity.
Unit III: Major Histocompatibility complexes – class I & class II MHC antigens,
antigen processing. Immunity to infection – immunity to different organisms,
pathogen defense strategies, avoidance of recognition. Autoimmune diseases,
Immunodeficiency-AIDS.
Unit IV: Vaccines & Vaccination – adjuvants, cytokines, DNA vaccines,
recombinant vaccines, bacterial vaccines, viral vaccines, vaccines to other
infectious agents, passive & active immunization. Introduction to
immunodiagnostics – RIA, ELISA.
 Kuby immunology, Judith A Owen; Jenni Punt; Sharon A Stranford; Patricia P
Jones; Janis Kuby. New York : W.H. Freeman, 2013.
Texts  K. Abbas and Lichtman, A. H. Basic Immunology – Functions and Disorders of
the Immune System, 3rd Edition (updated edition). Saunders-Elsevier.
Philadelphia, PA. 2011.
 SAGE University, Indore
 http://www.uth.tmc.edu/pathology/medic/immunology/immuntbl.htm
References  Parham, P. The Immune System. 3rd Edition. Garland Publishing, New York,
2009
On completion of this course a student should be able to understand
1. An understanding of humoral and cellular immunity and their relative
significances to transfusion science theory and practice.
Course 2. An understanding of the characteristics of antigens and antibodies.
Outcomes 3. An understanding of the nature of antigen-antibody reactions.
4. An appreciation of the importance of immunology as a foundation of transfusion
medicine theory and practice.
5. An understanding of the immunodiagnostics.

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 SAGE University, Indore
Institute of Biological Science Biotechnology
Code Title
B.Sc. IV Sem.
BBTIMY01P IMMUNOLOGY
L T/M P N C
Credit Score
- - 2 - 1
Prerequisites
BBTIMY01P; IMMUNOLOGY
(if any)
The purpose of the Immunology course is to provide a basic knowledge of the
immune response and its involvement in health and disease. An effort has been
Course made to increase clinical relevance and problem-solving skills through an essay
Objectives
assignment and faculty presented clinical correlations, and a team based learning
exercise.
1. Differential leucocytes count
2. Total leucocytes count
3. Total RBC count
4. Haemagglutination assay
Course
Content 5. Haemagglutination inhibition assay
6. Separation of serum from blood
7. Double immunodiffusion test using specific antibody and antigen.
8. ELISA.
On completion of this course a student should be able to understand
1. An understanding of humoral and cellular immunity and their relative
significances to transfusion science theory and practice.
Course 2. An understanding of the characteristics of antigens and antibodies.
Outcomes 3. An understanding of the nature of antigen-antibody reactions.
4. An appreciation of the importance of immunology as a foundation of transfusion
medicine theory and practice.
5. An understanding of the immunodiagnostics.
 SAGE University, Indore
Institute of Biological Science Biotechnology
Code Title
B.Sc. IV Sem.
BBTBIF01T BIOINFORMATICS
L T/M P N C
Credit Score
3 1 - - 4
Prerequisites
BBTBIF01T; BIOINFORMATICS
(if any)
Bioinformatics is defined broadly as the study of the inherent structure of
biological information. It is the marriage of biology and the information sciences.
Examples of current bioinformatics research include the analysis of gene and
protein sequences to reveal protein evolution and alternative splicing, the
Course development of computational approaches to study and predict protein structure to
Objectives further understanding of function, the analysis of mass spectrometry data to
understand the connection between phosphorylation and cancer, the development of
computational methods to utilize expression data to reverse engineer gene networks
in order to more completely model cellular biology, and the study of population
genetics and its connection to human disease.
Unit I: History of Bioinformatics. The notion of Homology. Sequence Information
sources, EMBL, GENBANK, Entrez, Unigene, Understanding the structure of each
source and using it on the web.
Unit II: Introduction to data types and Source. Population and sample,
Classification and Presentation of Data. Quality of data, private and public data
sources. General Introduction of Biological Databases; Nucleic acid databases
(NCBI, DDBJ, and EMBL). Protein databases (Primary, Composite, and
Secondary). Specialized Genome databases: (SGD, TIGR, and ACeDB). Structure
databases (CATH, SCOP, and PDBsum)
Course Unit III: Protein Information Sources, PDB, SWISSPROT, TREMBL,
Content Understanding the structure of each source and using it on the web. Introduction of
Data Generating Techniques and Bioinformatics problem posed by them-
Restriction Digestion, Chromatograms, Blots, Microarrays, Mass Spectrometry.
Unit IV: Sequence and Phylogeny analysis, Detecting Open Reading Frames,
Outline of sequence Assembly, Mutation/Substitution Matrices, Pairwise
Alignments, Introduction to BLAST, using it on the web, Interpreting results,
Multiple Sequence Alignment, Phylogenetic Analysis.
Unit V: Searching Databases: SRS, Entrez, Sequence Similarity Searches-BLAST,
FASTA, Data Submission. Genome Annotation: Pattern and repeat finding, Gene
identification tools.
 SAGE University, Indore
 Introduction to Bioinformatics by Aurther M lesk
 Developing Bioinformatics Computer Skills By: Cynthia Gibas, Per Jambeck
Texts
 Pevsner J. (2009) Bioinformatics and Functional Genomics. II Edition. Wiley-
Blackwell.
 Campbell A. M., Heyer L. J. (2006) Discovering Genomics, Proteomics and
Bioinformatics. II Edition. Benjamin Cummings.
References
 Ghosh Z. and Bibekanand M. (2008) Bioinformatics: Principles and Applications.
Oxford University Press.
On completion of this course a student should be able to understand
1. Become familiar with the essential concepts of bioinformatics.
Course 2. Explore the history of this young area.
Outcomes 3. Experience how rapidly bioinformatics is growing.
4. Insilico Prediction.
5. Computational Biology.

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 SAGE University, Indore

Institute of Biological Science Biotechnology

Code Title
B.Sc. IV Sem.
BBTBIF01P BIOINFORMATICS
L T/M P N C
Credit Score
- - 2 - -
Prerequisites
BBTBIF01P; BIOINFORMATICS
(if any)
Bioinformatics is defined broadly as the study of the inherent structure of
biological information. It is the marriage of biology and the information sciences.
Examples of current bioinformatics research include the analysis of gene and
protein sequences to reveal protein evolution and alternative splicing, the
Course development of computational approaches to study and predict protein structure to
Objectives further understanding of function, the analysis of mass spectrometry data to
understand the connection between phosphorylation and cancer, the development of
computational methods to utilize expression data to reverse engineer gene networks
in order to more completely model cellular biology, and the study of population
genetics and its connection to human disease.
1. Sequence information resource
2. Understanding and use of various web resources: EMBL, Genbank, Entrez,
Unigene,
3. Protein information resource (PIR)

PRACTICALS 4. Understanding and using: PDB, Swissprot, TREMBL

5. Using various BLAST and interpretation of results.
6. Retrieval of information from nucleotide databases.
7. Sequence alignment using BLAST.
8. Multiple sequence alignment using Clustal W.
On completion of this course a student should be able to understand
1. Become familiar with the essential concepts of bioinformatics.
2. Explore the history of this young area.
Course
Outcomes 3. Experience how rapidly bioinformatics is growing.
4. Insilico Prediction.
5. Computational Biology.
 SAGE University, Indore

Institute of Biological Science Biotechnology

Code Title
B.Sc. IV Sem.
BBTEEM01T ECOLOGY AND ENVIRONMENT MANAGEMENT
L T/M P N C
Credit Score
- - 2 - 3
Prerequisites
BBTEEM01T: ECOLOGY AND ENVIRONMENT MANAGEMENT
(if any)
The course is centrally concerned with understanding deliberate efforts to translate
Course environmental knowledge into action in order to achieve particular outcomes in the
Objectives way landscapes, societies and/or natural ecosystems are used and managed.
Unit I: Our Environment: Geological consideration of Atmosphere, Hydrosphere,
Lithosphere. Scope of Ecology.
Unit II: Development & Evolution of Ecosystem. Principles & Concepts of
Ecosystem. Structure of ecosystem. Strata of an ecosystem. Types of ecosystem
including habitats. Cybernetics & Homeostasis. Biological control of chemical
environment.
Unit III: Energy transfer in an Ecosystem. Food chain, food web, Energy budget,
Course Production & decomposition in a system. Ecological efficiencies, Trophic structure
Content & energy pyramids, Ecological energetic, principles pertaining to limiting factors,
Bio-geochemical cycles (N,C,P cycles).
Unit IV: Pollution & environmental Health related to Soil, Water, Air, Food,
Pesticides, Metals, Solvents, Radiations, Carcinogen, Poisons. Detection of
Environmental pollutant. Indicators & detection systems. Bio-transformation,
Plastic, Aromatics, Hazardous wastes Environmental cleanup : Case studies
Unit V: Environmental biotechnologies, Biotechnologies in protection and
preservation of environment. Bioremediation, Waste disposal.
 Chapman, J.L., Reiss, M.J. 1999. Ecology: Principles and applications (2nd
edition) Cambridge University Press.
 Divan Rosencraz, Environmental laws and policies in India, Oxford Publication.
 Ghosh, S.K., Singh, R. 2003. Social forestry and forest management. Global
Texts Vision Publishing House
 Joseph, B., Environmental studies, Tata Mc Graw Hill.
 Michael Allabay, Basics of environmental science, Routledge Press.
 Miller, G.T. 2002. Sustaining the earth, an integrated approach. (5th edition)
Books/Cole, Thompson Learning, Inc. Thompson Learning, Inc
 SAGE University, Indore
 Mohapatra Textbook of environmental biotechnology IK publication.
 Rana SVS, Environmenta lpollution – health and toxicology, Narosa Publication
References  Sinha, S. 2010. Handbook on Wildlife Law Enforsement in India. TRAFFIC,
India.
 Thakur, I S, Environmental Biotechnology, I K Publication.
On completion of this course a student should be able to understand:
1. Environmental management approaches.
2. The ability to analyze environmental management
Course 3. Learn the major principles of sustainable development,
Outcomes
4. Gain the knowledge of Biodiversity conservation etc.
5. Environmental education and to enhance student’s knowledge in environmental
biotechnologies.

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 SAGE University, Indore

Institute of Biological Science Biotechnology

Code Title
B.Sc. IV Sem.
BBTEEM01P ECOLOGY AND ENVIRONMENT MANAGEMENT
L T/M P N C
Credit Score
- - 2 - 1
Prerequisites
BBTEEM01P: ECOLOGY AND ENVIRONMENT MANAGEMENT
(if any)
The course is centrally concerned with understanding deliberate efforts to translate
Course environmental knowledge into action in order to achieve particular outcomes in the
Objectives way landscapes, societies and/or natural ecosystems are used and managed.
1. Study of all the biotic and abiotic components of any simple ecosystem- natural
pond or terrestrial ecosystem or human modified ecosystem.
2. Determination of population density in a terrestrial community or hypothetical
community by quad rate method and calculation of the Simpson’s and Shannon-

PRACTICALS Weiner diversity index for the same community.

3. Principle of GPS (Global Positioning System).
4. Study of the types of soil, their texture by sieve method and rapid tests for –pH,
chlorides, nitrates, carbonates and organic carbon
5. Study any five endangered/ threatened species- one from each class.
On completion of this course a student should be able to understand:
1. Environmental management approaches.
2. The ability to analyze environmental management
Course 3. Learn the major principles of sustainable development,
Outcomes
4. Gain the knowledge of Biodiversity conservation etc.
5. Environmental education and to enhance student’s knowledge in environmental
biotechnologies.
 SAGE University, Indore
Institute of Biological Science Biotechnology
Code Title
B.Sc. V Sem.
BBTBPT01T BIOPROCESS TECHNOLOGY
L T/M P N C
Credit Score
3 1 - - 4
Prerequisites
BTBPT05T; BIOPROCESS TECHNOLOGY
(if any)
Course To acquaint students with technical and biological aspect of microbial utilisation
Objectives for production of metabolites.
Unit I: Introduction to bioprocess technology. Range of bioprocess technology
and its chronological development. Basic principle components of fermentation
technology. Types of microbial culture and its growth kinetics– Batch, Fedbatch
and Continuous culture.
Unit II: Design of bioprocess vessels- Significance of Impeller, Baffles, Sparger;
Types of culture/production vessels- Airlift; Cyclone Column; Packed Tower and
their application in production processes. Principles of upstream processing –
Course Media preparation, Inoculation development and sterilization.
Content
Unit III: Introduction to oxygen requirement in bioprocess; mass transfer
coefficient; factors affecting KLa. Bioprocess measurement and control system
with special reference to computer aided process control.
Unit IV: Introduction to downstream processing, product recovery and
purification. Effluent treatment.
Unit V: Microbial production of ethanol, amylase, lactic acid and Single Cell
Proteins.
 Shuler ML and Kargi F, Bioprocess Engineering, Prentice Hall (2004).
 Stanbury PF,Hall SJ and Whitaker A, Principles of Fermentation Technology,
Texts Butterworth –Heinemann 2005).
 Bailey JE and Ollis DF, Biochemical Engineering Fundamentals, Mc-Graw Hill,
Inc. (1986).
 Atkinson B and Mavituna F Biochemical Engineering and
References BiotechnologyHandbook, McGraw Hill (1993).
 Doran P M, Bioprocess Engineering Principles, Academic Press (1995)
On completion of this course a student should be able to understand:
1. Designing of bioreactors and control necessary for maximising production.
Course 2. Select and optimise media for maximum production of microbial metabolites.
Outcomes 3.Designing of protocols for strain improvement
4. Separation of molecules after fermentation process.
5. Conduct various researches both at basic and applied levels.
 SAGE University, Indore
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 SAGE University, Indore
Institute of Biological Science Biotechnology
Code Title
B.Sc. V Sem.
BBTBPT01P BIOPROCESS TECHNOLOGY
L T/M P N C
Credit Score
- - 2 - 1
Prerequisites
BTBPT05P; BIOPROCESS TECHNOLOGY
(if any)
Course To acquaint students with technical and biological aspect of microbial utilisation
Objectives for production of metabolites.
1. Bacterial growth curve.
2. Calculation of thermal death point (TDP) of a microbial sample.
3. Production and analysis of ethanol.
PRACTICALS
4. Production and analysis of amylase.
5. Production and analysis of lactic acid.
6. Isolation of industrially important microorganism from natural resource.
On completion of this course a student should be able to understand:
1. Designing of bioreactors and control necessary for maximising production.
Course 2. Select and optimise media for maximum production of microbial metabolites.
Outcomes 3.Designing of protocols for strain improvement
4. Separation of molecules after fermentation process.
5. Conduct various researches both at basic and applied levels.
 SAGE University, Indore
Institute of Biological Science Biotechnology
Code Title
B.Sc. V Sem.
BTABT01T ANIMAL BIOTECHNOLOGY
L T/M P N C
Credit Score
3 1 2 - 4/1
Prerequisites
BTABT05T; ANIMAL BIOTECHNOLOGY
(if any)
Course Understanding the principles of animal cell culture and its application
Objectives
Unit I: Introduction, importance, history of cell culture development, different
tissue culture techniques including primary and secondary culture, continuous cell
lines, suspension culture, organ culture, hybridoma technology, culture of
lymphocyte, oviductal, epithelial cell culture, stem cell and iPS cells
Unit II: Different type of cell culture media, growth supplements, serum free
media, balanced salt solution, other cell culture reagents, culture of different tissues
and its application. Gene transfer methods in Animals – Microinjection, Embryonic
Stem cell, gene transfer, Retrovirus & Gene transfer.
Course Unit III: Introduction to transgenesis. Transgenic Animals – Mice, Cow, Pig,
Content
Sheep, Goat, Bird, Insect. Animal diseases need help of Biotechnology – Foot-and
mouth disease, Coccidiosis,Trypanosomiasis, Theileriosis.
Unit IV: Animal propagation – Artificial insemination, Animal Clones.
Conservation Biology – Embryo transfer techniques. Introduction to Stem Cell
Technology and its applications.
Unit V: Genetic modification in Medicine - gene therapy, types of gene therapy,
vectors in gene therapy, molecular engineering, human genetic engineering,
problems & ethics.
 Freshney RI. 2005. Culture of Animal Cells. Wiley Liss.
Texts  Portner R. 2007. Animal Cell Biotechnology. Humana Press.
 Watson, J.D., M yers, R.M., Caudy, A. and Witkowski, J.K. (2007). Recombinant
DNAgenesand genomes- A short course. III Edition. Freeman and Co., N.Y.,
USA.
References
 Griffiths, A.J.F., J.H. Miller, Suzuki, D.T., Lewontin, R.C. and Gelbart, W.M.
(2009). An introduction to genetic analysis. IX Edition. Freeman & Co., N.Y.,
USA.
 SAGE University, Indore
On completion of this course a student should be able to understand
1. A detailed technical understanding of the key advanced methods used in the
contemporary biotechnology sector
2. An appreciation of how these techniques are applied both in biotechnology and
Course in advanced research
Outcomes
3. Acquired the knowledge to enable them to critically appraise new data arising
from the use of these techniques
4. To interpret the implications of useful data.
5. To have practical knowledge of subject.

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 SAGE University, Indore

Institute of Biological Science Biotechnology

Code Title
B.Sc. V Sem.
BTABT01P ANIMAL BIOTECHNOLOGY
L T/M P N C
Credit Score
- - 2 - 1
Prerequisites
BTABT01P; ANIMAL BIOTECHNOLOGY
(if any)
Course Understanding the principles of animal cell culture and its application
Objectives
1. Sterilization techniques: Theory and Practical: Glass ware sterilization, Media
sterilization, Laboratory sterilization
2. Sources of contamination and decontamination measures.
3. Preparation of Hanks Balanced salt solution

PRACTICALS 4. Preparation of Minimal Essential Growth medium

5. Isolation of lymphocytes for culturing
6. DNA isolation from animal tissue
7. Quantification of isolated DNA.
8. Resolving DNA on Agarose Gel.
On completion of this course a student should be able to understand
1. A detailed technical understanding of the key advanced methods used in the
contemporary biotechnology sector
2. An appreciation of how these techniques are applied both in biotechnology and
Course in advanced research
Outcomes
3. Acquired the knowledge to enable them to critically appraise new data arising
from the use of these techniques
4. To interpret the implications of useful data.
5. To have practical knowledge of subject.
 SAGE University, Indore

Institute of Biological Science Biotechnology

Code Title
B.Sc. V Sem.
BBTRDT01T RECOMBINANT DNA TECHNOLOGY
L T/M P N C
Credit Score
3 1 - - 4
Prerequisites
BBTRDT05T; RECOMBINANT DNA TECHNOLOGY
(if any)
To familiarize the student with emerging field of biotechnology i.e. Recombinant
Course DNA Technology as well as to create understanding and expertise in wet lab
Objectives
techniques in genetic engineering.
Unit I: Molecular tools and applications- restriction enzymes, ligases, polymerases,
alkaline phosphatase. Gene Recombination and Gene transfer: Transformation,
Episomes, Plasmids and other cloning vectors (Bacteriophage-derived vectors,
artificial chromosomes), Microinjection, Electroporation, Ultrasonication, Principle
and applications of Polymerase chain reaction (PCR), primer-design, and RT-
(Reverse transcription) PCR.
Unit II: Restriction and modification system, restriction mapping. Southern and
Northern hybridization. Preparation and comparison of Genomic and cDNA
library, screening of recombinants, reverse transcription,. Genome mapping, DNA
fingerprinting, Applications of Genetic Engineering.
Course Unit III: Genetic engineering in animals: Production and applications of transgenic
Content
mice, role of ES cells in gene targeting in mice, Therapeutic products produced by
genetic engineering-blood proteins, human hormones, immune modulators and
vaccines.
Unit IV: Random and site-directed mutagenesis: Primer extension and PCR based
methods of site directed mutagenesis, Random mutagenesis, Gene shuffling,
production of chimeric proteins, Protein engineering concepts and examples.
Unit V: Genetic engineering in plants: Use of Agrobacterium tumefaciens and A.
rhizogenes, Ti plasmids, Strategies for gene transfer to plant cells, Direct DNA
transfer to plants, Gene targeting in plants, Use of plant viruses as episomal
expression vectors.
 Brown, T. (2010). Gene cloning and DNA analysis: an introduction. John Wiley
& Sons.
Texts
 Primrose, S. B., & Twyman, R. (2009). Principles of gene manipulation and
genomics. Wiley. com.
 SAGE University, Indore
 Howe, C. J. (2007). Gene cloning and manipulation. Cambridge University Press.
References  Principles of Gene Manipulation & Genomics – 7th Edition – Sandy B.Primrose,
Richard Twyman– Blackwell
On completion of this course a student should be able to understand
1. List out tools used for gene exploration.
Course 2. Utilize the knowledge on creation of a genomic library.
Outcomes 3. Recall about transgenic plants & animals
4. To understand the applications of Genetic Engineering.
5. To understand the process of gene regulation.

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 SAGE University, Indore

Institute of Biological Science Biotechnology

Code Title
B.Sc. V Sem.
BBTRDT01P RECOMBINANT DNA TECHNOLOGY
L T/M P N C
Credit Score
- - 2 - 1
Prerequisites
BBTRDT01P; RECOMBINANT DNA TECHNOLOGY
(if any)
To familiarize the student with emerging field of biotechnology i.e. Recombinant
Course DNA Technology as well as to create understanding and expertise in wet lab
Objectives
techniques in genetic engineering.
Isolation of chromosomal DNA from plant cells
2. Isolation of chromosomal DNA from E.coli
3. Qualitative and quantitative analysis of DNA using spectrophotometer
4. Plasmid DNA isolation
PRACTICALS
5. Restriction digestion of DNA
6. Making competent cells
7. Transformation of competent cells.
8. Demonstration of PCR
On completion of this course a student should be able to understand
1. List out tools used for gene exploration.
Course 2. Utilize the knowledge on creation of a genomic library.
Outcomes 3. Recall about transgenic plants & animals
4. To understand the applications of Genetic Engineering.
5. To understand the process of gene regulation.
 SAGE University, Indore
Institute of Biological Science Biotechnology
Code Title
B.Sc. V Sem.
BTDDG01T DRUG DESIGNING
L T/M P N C
Credit Score
3 1 - - 4
Prerequisites
BTDDG01T; DRUG DESIGNING
(if any)
Students learn principles that govern the process of modern drug discovery and
Course development. Students will follow a path similar to that taken by real-life drug
Objectives developers by learning important elements of the drug design process in a logical
order.
Unit I: Introduction to The Drug Discovery/Development: Drug Discovery, Drug
Development, Source of Drugs, Structural effects on drug action.
Unit II: Approaches to New Drug Discovery: Drugs Derived from Natural
Products, Existing Drugs as a Source for New Drug Discovery, Screening for New
Drug Leads, Modern “Rational Approach” to Drug Design.
Course Unit III: Enzymes as Targets of Drug Design: Enzyme kinetics, Enzyme inhibition
Content and activation, Approaches to the Rational Design of Enzyme Inhibitors.
Unit IV: Receptors as Targets of Drug Design: Receptor Theory, Receptor
Complexes and Allosteric Modulators, Molecular Biology of Receptors, Lead
Compound Discovery of Receptor agonists and antagonists
Unit V: Computer-Aided Drug Design: Docking and virtual screening, Molecular
Dynamics and binding free energy methods
 Advanced Concepts in Structural Bioinformatics: Structural Bioinformatics:
Philip E. Bourne (Editor), HelgeWeissig (Editor).
 Protein Structure Prediction: A Practical Approach (The Practical Approach
Series , No 170) by Michael J. E. Sternberg
Texts  Computer-aided Drug Design: Practical Application of Computer-Aided Drug
Design (Hardcover) by Charifson (Author)
 Computer-Aided Drug Design. Methods and Applications. Edited by Thomas J.
Perun and C. L. Propst Marcel Dekker

 The organic chemistry of drug design by Richard B. Silverman. Second edition,

Elsevier, 2004
References
 An introduction to Medicinal Chemistry by Graham L. Patrick. Fourth edition,
Oxford, 2009
 SAGE University, Indore
On completion of this course a student should be able to understand
1. Be able to discuss the pharmacological management of infectious diseases
including the mechanism of action of specific agents and their structure activity
relationships.
2. Understand key principles of pharmacognosy and natural products and their role
in shaping the pharmaceutical industry, including Traditional, Complementary
and Alternative Medicines.
Course 3. Understand the role of synthetic chemistry in the development of
Outcomes pharmaceutical agents; and the modification of chemical structures to develop
new drug molecules.
4. Have an advanced understanding of the chemical structure of a pharmaceutical
agent and determine the chemical group/s responsible for a given biological
effect.
5. Be able to describe the modern and innovative discovery of biopharmaceuticals
as it relates to today's healthcare and future trends in modern drug discovery
globally.

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 SAGE University, Indore
Institute of Biological Science Biotechnology
Code Title
B.Sc. VI Sem.
BBTBAT01T BIO ANALYTICAL TOOLS
L T/M P N C
Credit Score
3 1 - - 4
Prerequisites
BBTBAT01T; BIO ANALYTICAL TOOLS
(if any)
The primary objectives of this course are to develop the skills to understand the
Course theory and practice of bioanalytical techniques. To provide scientific understanding
Objectives of analytical techniques and detail interpretation of results.

Unit I: Introduction, Modern approaches in Bioanalysis and Bioassays.

Unit II: Simple microscopy, phase contrast microscopy, florescence and electron
microscopy (TEM andSEM), pH meter, absorption and emission spectroscopy.
Unit III: Principle and law of absorption fluorimetry, colorimetry,
spectrophotometry (visible, UV, infrared), centrifugation, cell fractionation
techniques, isolation of sub-cellular organelles and particles.
Course Unit IV: Introduction to the principle of chromatography. Paper chromatography,
Content
thin layer chromatography, column chromatography: silica and gel filtration,
affinity and ion exchange chromatography, gas chromatography, HPLC.
Unit V: Introduction to electrophoresis. polyacrylamide gel (native and SDS-
PAGE), agarose-gel electrophoresis, pulse field gel electrophoresis, immuno-
electrophoresis, isoelectric focusing, Western blotting.
Introduction to Biosensors and Nanotechnology and their applications.
 D. Campbell, Biological spectroscopy (Benjamin/Cummings Pub. Co, Menlo
Park, Calif, 1984), Biophysical techniques series
 K. Wilson, J. M. Walker, Eds., Principles and techniques of biochemistry and
molecular biology (Cambridge University Press, Cambridge, UK: New York, 7th
ed., 2009)
 R. F. Boyer, Biochemistry laboratory: modern theory and techniques (Prentice
Texts Hall, Boston, 2nd ed., 2012).
 R. Katoch, Analytical techniques in biochemistry and molecular biology
(Springer, New York, 2011).
 D. L. Spector, R. D. Goldman, Eds., Basic methods in microscopy: protocols and
concepts from cells: a laboratory manual (Cold Spring Harbor Laboratory Press,
Cold Spring Harbor, N.Y, 2006).
 J. R. Lakowicz, Principles of fluorescence spectroscopy (Springer, New York,
 SAGE University, Indore
2006; http://site.ebrary.com/id/10229235).
 R. F. Boyer, Modern experimental biochemistry (Benjamin Cummings, San
Francisco, 3rd ed., 2000).
References
 R. L. Switzer, Experimental biochemistry (W. H. Freeman and Co, New York,
3rd ed., 1999).
On completion of this course a student should be able to understand
1. To be able to use selected analytical techniques.
2. Familiarity with working principals, tools and techniques of analytical
Course techniques.
Outcomes 3. To understand the strengths, limitations and creative use of techniques for
problem solving.
4. To understand Nanotechnology and their applications.
5. To understand bioassays.

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 SAGE University, Indore
Institute of Biological Science Biotechnology
Code Title
B.Sc. VI Sem.
BBTBAT01P BIO ANALYTICAL TOOLS
L T/M P N C
Credit Score
- - 2 - 1
Prerequisites
BBTBAT01P; BIO ANALYTICAL TOOLS
(if any)
The primary objectives of this course are to develop the skills to understand the
Course theory and practice of bioanalytical techniques. To provide scientific understanding
Objectives of analytical techniques and detail interpretation of results.

1. Native gel electrophoresis of proteins

2. SDS-polyacrylamide slab gel electrophoresis of proteins under reducing
conditions.
3. Preparation of the sub-cellular fractions of rat liver cells.

PRACTICALS 4. Preparation of protoplasts from leaves.

5. Separation of amino acids by paper chromatography.
6. To identify lipids in a given sample by TLC.
7. To verify the validity of Beer’s law and determine the molar extinction
coefficient.
On completion of this course a student should be able to understand
1. To be able to use selected analytical techniques.
2. Familiarity with working principals, tools and techniques of analytical
Course techniques.
Outcomes 3. To understand the strengths, limitations and creative use of techniques for
problem solving.
4. To understand Nanotechnology and their applications.
5. To understand bioassays.
 SAGE University, Indore
Institute of Biological Science Biotechnology
Code Title
B.Sc. VI Sem.
BBTGAP01T GENOMICS AND PROTEOMICS
L T/M P N C
Credit Score
3 1 - - 4
Prerequisites
BBTGAP01T; GENOMICS AND PROTEOMICS
(if any)
Course Explain how the field of genomics led to the development of proteomics
Objectives
Unit I: Introduction and scope of proteomics; Protein separation techniques: ion-
exchange, size-exclusion and affinity chromatography techniques, Polyacrylamide
gel electrophoresis; Isoelectric focusing (IEF); Two dimensional PAGE for
proteome analysis; Image analysis of 2D gels
Unit II: Introduction to mass spectrometry; Strategies for protein identification;
Protein sequencing; Protein modifications and proteomics; Applications of
proteome analysis to drug; Protein-protein interaction.
Course Unit III: Protein engineering; Protein chips and functional proteomics; Clinical
Content
and biomedical application of proteomics; Proteome database; Proteomics industry.
Unit IV: Methods of preparing genomic DNA; DNA sequence analysis methods:
Sanger Dideoxy method and Fluorescence method. Gene variation and Single
Nucleotide Polymorphisms (SNPs); Expressed sequenced tags (ESTs); Gene-
disease association.
Unit V: Recombinant DNA technology: DNA cloning basics, Polymerase chain
reaction, DNA fingerprinting, Human genome project and the genetic map.
 Brown T. A. 2007, Genomes 3. Garland Science Publishing, New York.
 Dunham, I., 2003. Genome Mapping and sequencing. Horizon Scientific
 Graur, D and W H Li, 2000. Fundamentals of molecular evolution. Sinauer
Texts Associates.
 Hartwell, L. H., L. Hood, M. L. Goldberg, A. E. Reynolds, L. M. Silver and R. G.
Veres. 2004. Genetics from Genes to Genomes. McGraw Hill.
 Lewin B. 2003. Genes VIII. Oxford University Press. Oxford.
 The Human Genome 2001, Nature Vol. 409
References  Primrose, S. B., and R. M. Twyman . 2006. Principles of gene manipulation and
Genomics, Blackwell Publishing MA. USA
 SAGE University, Indore
On completion of this course a student should be able to understand
1. Infer the basic concepts of genomics, transcriptomics and proteomics.
2. List and discuss the use of genomics and proteomics in human health
Course 3. Suggest and outline solution to theoretical and experimental problems in
Outcomes Genomics field.
4. Experience how rapidly bioinformatics makes the growth of genomics and
proteomics.
5. To understand Genome Mapping and sequencing.

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 SAGE University, Indore
Institute of Biological Science Biotechnology
Code Title
B.Sc. VI Sem.
BBTBSS01P BIOSTATISTICS
L T/M P N C
Credit Score
3 1 - - 4
Prerequisites
BBTBSS06T; BIOSTATISTICS
(if any)
A degree in biostatistics prepares students for work in a wide variety of challenging
Course positions in government, industry, and education. Graduates have found careers
Objectives involving teaching, research, and consulting in such fields as medicine, public
health, life sciences, and survey research
Unit I: Data, Uncertainty, and need of statistics, Descriptive statistics; Elementary
concepts in Statistics: Concepts of statistical population and sample from a
population; qualitative and quantitative data; nominal, ordinal, ratio, interval data;
cross sectional and time series data; discrete and continuous data.
Unit II: Statistical analysis using R, Correlation and dependence Week. Basic
fitting and regression. Probability, Probability distribution. Sampling distributions,
The Central Limit Theorem, students test, sample size
Unit III: Collection and scrutiny of data: Primary data; designing a questionnaire
and a scheule; secondary data and sources of secondary data.
Course ANOVA, Experiment design, Multiple regression analysis; Principle component
Content analysis, maximum likelihood, Bayesian inference.
Unit IV: Testing of hypothesis:Basic concepts, simple and composite hypotheses,
two types of errors, critical region, significance level, size and power of the test, p-
value and its interpretation. Neymann-Pearson Lemma (Statement only) and its
application in testing of hypothesis.
Unit V: Bioassays:Types of biological assays, direct assays, ratio estimators,
asymptotic distributions, regression approaches for estimating dose response
relationships. Quantal responses, methods of estimation of parameters, dose
allocation schemes, median dose, estimation of points on the quantal response
function, Estimation of safe doses.
 Fundamentals of Biostatistics. by Irfan A Khan.
 An introduction to Biostatistics. by PSS Sunder Rao.
Texts
 Introduction to the Practice of Statistics by Moore and McCabe
 SAGE University, Indore
 Principles of Biostatistics. Marcello Pagano.
References  Course Manuals: S-PLUS Command Line Essentials, The Analysis
of Microarrays.
On completion of this course a student should be able to understand
1. Specify the design for a basic clinical trial, with randomization, blinding,
masking and a control group
2. Explain designs for repeated measures and cross-over trials
Course 3. Explain and apply basic survival analysis models.
Outcomes
4. Elementary concepts in Statistics.
5. A detailed technical understanding of key advanced methods used in the
contemporary biotechnology sector.

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 SAGE University, Indore

Institute of Biological Science Biotechnology

Code Title
B.Sc. VI Sem.
BBTBSS01P BIOSTATISTICS
L T/M P N C
Credit Score
- - 2 - 1
Prerequisites
BBTBSS01P; BIOSTATISTICS
(if any)
A degree in biostatistics prepares students for work in a wide variety of challenging
Course positions in government, industry, and education. Graduates have found careers
Objectives involving teaching, research, and consulting in such fields as medicine, public
health, life sciences, and survey research
1. Based on graphical Representation
2. Based on measures of Central Tendency & Dispersion
PRACTICALS
3. Based on Distributions Binomial Poisson Normal
4. Based on t, f, z and Chi-square
On completion of this course a student should be able to understand
1. Specify the design for a basic clinical trial, with randomization, blinding,
masking and a control group
2. Explain designs for repeated measures and cross-over trials
Course 3. Explain and apply basic survival analysis models.
Outcomes
4. Elementary concepts in Statistics.
5. A detailed technical understanding of key advanced methods used in the
contemporary biotechnology sector.