Agilent 1260 Infinity

Binary Pump

User Manual

Agilent Technologies
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1260 Infinity Binary Pump User Manual

 In This Guide...

In This Guide...

This manual covers the Agilent 1260 Infinity Binary Pump (G1312B).

1 Introduction to the Binary Pump

This chapter gives an introduction to the module and an instrument
overview.

2 Site Requirements and Specifications

This chapter provides information about site requirements and
specifications for the binary pump.

3 Installing the Pump

This chapter gives information about the preferred stack setup for your
system and the installation of your binary pump.

4 Using the Pump

This chapter explains the operational parameters of the binary pump.

5 Optimizing Performance
This chapter gives information on how to optimize the performance of the
Binary Pump under special operational conditions.

6 Troubleshooting and Diagnostics

Overview of the troubleshooting and diagnostic features.

7 Error Information
This chapter describes the meaning of error messages, and provides
information on probable causes and suggested actions how to recover from
error conditions.

1260 Infinity Binary Pump User Manual 3

In This Guide...

8 Test Functions and Calibration

This chapter explains all test functions that are available for the binary
pump.

9 Diagnostic Signals
This chapter explains all diagnostic signals of the binary pump.

10 Maintenance
This chapter describes the maintenance of the module.

11 Parts and Materials for Maintenance

This chapter lists all parts and tools that are required for maintenance.

12 Identifying Cables
This chapter provides information on cables used with the Agilent 1200
Infinity Series modules.

13 Hardware Information
This chapter provides detailed technical information about your binary
pump.

14 Appendix
This appendix provides general safety and environmental information.

4 1260 Infinity Binary Pump User Manual

 Contents

Contents

1 Introduction to the Binary Pump 9

Instrument and Operation 10
Overview of the Hydraulic Path 13
Leak and Waste Handling 18

2 Site Requirements and Specifications 21

Site Requirements 22
Physical Specifications 25
Performance Specifications 26

3 Installing the Pump 29

Unpacking the Binary Pump 30
Optimizing the Stack Configuration 33
Installation Information on Leak and Waste Handling 41
Installing the Binary Pump 45
Flow Connections with Solvent Selection Valve 48
Flow Connections without Solvent Selection Valve 51
Priming the System 54

4 Using the Pump 59

Leak and Waste Handling 60
Hints for Successful Use of the Binary Pump 61
Setting up the Pump with the G4208A Instant Pilot 62
Setting up the Agilent 1260 Infinity Binary Pump G1312B with the Instrument
Control Interface 63
Solvent Information 69
Algae Growth in HPLC Systems 74
Prevent Blocking of Solvent Filters 76

1260 Infinity Binary Pump User Manual 5

Contents

5 Optimizing Performance 79
When to Use a Vacuum Degasser 80
When to Use the Active Seal Wash Option 81
When to Use Alternative Seals 82
When to Use the Low Volume Mixer 83
When to Remove Damper and Mixer 84
How to Optimize the Compressibility Compensation Setting 87

6 Troubleshooting and Diagnostics 91

Overview of the Module’s Indicators and Test Functions 92
Status Indicators 94
User Interfaces 96
Agilent Lab Advisor Software 97

7 Error Information 99
What Are Error Messages 101
General Error Messages 102
Module Error Messages 109

8 Test Functions and Calibration 125

(System) Pressure Test 126
Valve Test 131
Binary Pump Solvent Compressibility Calibration 133
Pump Elasticity Calibration 135
Leak Rate Test 137

9 Diagnostic Signals 143

Analog Pressure Output 144
Diagnostic Signals in Chromatographic Data Systems 145

6 1260 Infinity Binary Pump User Manual

 Contents

10 Maintenance 147
Introduction to Maintenance and Repair 148
Warnings and Cautions 149
Cleaning the Module 151
Overview of Maintenance and Simple Repairs 152
Maintenance Procedures 153
Exchanging the Purge Valve Frit or the Purge Valve 154
Removing the Pump Head Assembly 157
Maintenance of a Pump Head without Seal Wash 159
Maintenance of a Pump Head with Seal Wash 163
Reinstalling the Pump Head Assembly 167
Seal Wear-in Procedure 169
Exchanging the Active Inlet Valve (AIV) or its Cartridge 170
Exchanging the Outlet Valve 173
Installation of the Solvent Selection Valve Upgrade Kit 175
Exchanging the Solvent Selection Valve 177
Exchanging the Optional Interface Board 180
Replacing Module Firmware 181

11 Parts and Materials for Maintenance 183

Hydraulic Path with Solvent Selection Valve 184
Hydraulic Path without Solvent Selection Valve 186
Pump Head Assembly Without Seal Wash 188
Pump Head Assembly with Seal Wash Option 190
Outlet Valve 192
Purge Valve Assembly 193
Active Inlet Valve Assembly 194
HPLC Starter Kit G4201-68707 195
HPLC Starter Kit G4202-68707 195
HPLC System Tool Kit 196
Bottle Head Assembly 197
Solvent Cabinet 198

1260 Infinity Binary Pump User Manual 7

Contents

12 Identifying Cables 201

Overview 202
Analog cables 204
Remote Cables 206
BCD Cables 209
CAN/LAN Cables 211
External Contact Cables 212
RS-232 Cable Kit 213
Agilent 1200 Module to Printer 214

13 Hardware Information 215

Firmware Description 216
Electrical Connections 219
Interfaces 221
Setting the 8-bit Configuration Switch (without On-board) LAN 228
Optional Interface Boards 232
The Solvent Selection Valve (SSV) 236
Early Maintenance Feedback 237
Instrument Layout 238

14 Appendix 239
General Safety Information 240
The Waste Electrical and Electronic Equipment Directive 243
Lithium Batteries Information 244
Radio Interference 245
Sound Emission 246
Agilent Technologies on the Internet 247

8 1260 Infinity Binary Pump User Manual

 1260 Infinity Binary Pump User Manual

1
Introduction to the Binary Pump
Instrument and Operation 10
Introduction to the Pump 10
Principle of Operation 11
Overview of the Hydraulic Path 13
Leak and Waste Handling 18

This chapter gives an introduction to the module and an instrument overview.

Agilent Technologies 9
1 Introduction to the Binary Pump
Instrument and Operation

Instrument and Operation

Introduction to the Pump
The binary pump comprises two identical pumps integrated into one
housing. Binary gradients are created by high- pressure mixing. An
optional degasser is available for applications that require best flow
stability, especially at low flow rates, for maximum detector sensitivity.
Pulse damper and mixer can be bypassed for low flowrate applications or
whenever a minimal transient volume is desirable. Typical applications are
high throughput methods with fast gradients on high resolution 2.1 mm
columns. The pump is capable of delivering flow in the range of 0.1 –
5 mL/min against up to 600 bar. A solvent selection valve (optional)
allows to form binary mixtures (isocratic or gradient) from one of two
solvents per channel. Active seal wash (optional) is available for use with
concentrated buffer solutions.

Outlet valve

Purge valve

Active inlet valve

Mixer

Figure 1 Overview of the binary pump

10 1260 Infinity Binary Pump User Manual

 Introduction to the Binary Pump 1
Instrument and Operation

Principle of Operation
The binary pump is based on a two- channel, dual- piston in- series design
which comprises all essential functions that a solvent delivery system has
to fulfill. Metering of solvent and delivery to the high- pressure side are
performed by two pump assemblies which can generate pressure up to
600 bar.
Each channel comprises a pump assembly including pump drive, pump
head, active inlet valve with replaceable cartridge, and outlet valve. The
two channels are fed into a low- volume mixing chamber which is
connected via a restriction capillary coil to a damping unit and a mixer. A
pressure sensor monitors the pump pressure. A purge valve with
integrated PTFE frit is fitted to the pump outlet for convenient priming of
the pumping system.

Ejg\ZkVakZ 9VbeZg
B^mZg
EjbedjiaZi

EgZhhjgZ
hZchdg
idlVhiZ

DjiaZi DjiaZi
kVakZ kVakZ

>caZikVakZ >caZikVakZ
B^m^c\
8]VbWZg
[gdb [gdb
hdakZci HZVah HZVah hdakZci
WdiiaZ6 WdiiaZ7

E^hidch E^hidch

Ejbe]ZVY6 Ejbe]ZVY7
Figure 2 The Hydraulic Path of the Binary Pump with Damper and Mixer

1260 Infinity Binary Pump User Manual 11

1 Introduction to the Binary Pump
Instrument and Operation

Damper and mixer can be bypassed for lowest delay volume of the binary
pump. This configuration is recommended for low flow rate applications
with steep gradients, see the Agilent 1260 Infinity Binary LC System
Manual.
Figure 3 on page 12 illustrates the flow path in low delay volume mode.
For instructions on how to change between the two configurations, see
“Convert the Binary Pump to Low Delay Volume Mode” on page 85.

Bypassing the mixer while the damper remains in line is not a supported configuration and
NOTE may lead to undesired behavior of the binary pump.

B^mZg 9VbeZg

Ejg\ZkVakZ

EjbedjiaZi EgZhhjgZ
hZchdg

idlVhiZ
DjiaZikVakZ
DjiaZi
kVakZ

>caZi
kVakZ >caZikVakZ
B^m^c\X]VbWZg
[gdbhdakZci HZVah HZVah [gdbhdakZci
WdiiaZ6 WdiiaZ7

E^hidch E^hidch

Ejbe]ZVY6 Ejbe]ZVY7

Figure 3 The Hydraulic Path of the Binary Pump with Bypassed Damper and Mixer

For pump specifications, see “Performance Specifications” on page 26.

12 1260 Infinity Binary Pump User Manual

 Introduction to the Binary Pump 1
Overview of the Hydraulic Path

Overview of the Hydraulic Path

The solvent from the bottle in the solvent cabinet enters the pump through
an active inlet valve. Each side of the binary pump comprises two
substantially identical pump units. Both pump units comprise a ball- screw
drive and a pump head with two sapphire pistons for reciprocating
movement.

Idb^m^c\X]VbWZg
>caZi DjiaZi
kVakZ kVakZ

;gdbhdakZciWdiiaZ

HZVa

E^hidc

7VaahXgZlYg^kZ

<ZVg

Bdidgl^i]ZcXdYZg

Figure 4 Pump head

1260 Infinity Binary Pump User Manual 13

1 Introduction to the Binary Pump
Overview of the Hydraulic Path

A servo- controlled variable reluctance motor drives the two ball- screw
drives in opposite directions. The gears for the ball- screw drives have
different circumferences (ratio 2:1) allowing the first piston to move at
double the speed of the second piston. The solvent enters the pump heads
close to the bottom limit and leaves it at its top. The outer diameter of
the piston is smaller than the inner diameter of the pump- head chamber
allowing the solvent to fill the gap in between. The first piston has a
stroke volume in the range of 20 µL to 100 µL depending on the flow
rate. The microprocessor controls all flow rates in a range of 1 µL/min to
5 mL/min. The inlet of the first pumping unit is connected to the active
inlet valve which is processor- controlled opened or closed allowing solvent
to be drawn into the first pump unit.
The outlet of the first pump chamber is connected by a 500 µL absorber
capillary to the second pump chamber. The outlets of the second chambers
of both pump channels joined via a small mixing chamber. A coiled
restriction capillary connects the mixing chamber via a pressure pulse
damper, a mixer and a pressure sensor to the purge valve assembly. The
outlet of the purge valve assembly is then connected to the attached
chromatographic system.
When turned on, the pump runs through an initialization procedure to
determine the upper dead center of the first piston of both pump
channels. The first piston moves slowly upwards to the mechanical stop of
the pump head and from there it moves back a predetermined path
length. The controller stores this piston position in memory. After this
initialization the pump starts operation with the set parameters for the
two pump channels.
The active inlet valve is opened and the down moving piston draws
solvent into the first pump head. At the same time the second piston is
moving upwards delivering into the system. After a controller defined
stroke length (depending on the flow rate) the drive motors are stopped
and the active inlet valve is closed. The motor direction is reversed and
moves the first piston up until it reaches the stored upper limit and at the
same time moving the second piston downwards.
Then the sequence starts again moving the pistons up and down between
the two limits. During the delivery stroke of the first piston the solvent in
the pump head is pressed through the outlet valve into the second
pumping unit. The second piston draws in half of the volume displaced by
the first piston and the remaining half volume is directly delivered into
the system. During the drawing stroke of the first piston, the second
piston delivers the drawn volume into the system.
For pump specifications, see “Performance Specifications” on page 26.

14 1260 Infinity Binary Pump User Manual

 Introduction to the Binary Pump 1
Overview of the Hydraulic Path

What is Pump Elasticity Compensation?

The flow path of the pump consists of pump chambers, sapphire pistons,
polymer seals, stainless steel tubing of different dimension, pressure
sensor, and so forth. All of these parts deform when pressurized. The sum
of this deformation is called pump elasticity.
Let us look at a practical example: Piston 1 draws solvent at ambient
pressure. The movement direction is reversed and the piston 1 now
compresses the solvent until the operating pressure of the HPLC system is
reached. The outlet valve opens, and solvent is pumped by piston 1 into
pump chamber 2. Due to two factors, the solvent volume that is delivered
into the system at high pressure is smaller than it is supposed to be:
1 The solvent is compressible
2 The pump has a certain elasticity which causes its internal volume to
increase with pressure.
In order to compensate for these two influences, their contributions must
be known. An elasticity calibration allows separating pump properties
from solvent properties and therefore allows transferring solvent
properties, which have been obtained from one pump to another pump
with different elasticity.
Elasticity calibration is done with a solvent, which properties
(compressibility, thermal expansion) are well- known and documented:
pure water. When pumping water and using its property data for
controlling the pump, any deviations from the theoretical pressure profile
during solvent recompression are caused by the elasticity of the pump.
The Pump Elasticity Calibration calculates correction factors to
compensate for the individual elasticity of the pump that is being
calibrated. The elasticity is different for every pump and may change with
the replacement of parts in the flow path, e.g. pump seals.
All binary pumps are elasticity calibrated at the factory and require
recalibration only after preventive maintenance or major repairs to the
flow path. Replacement of capillaries or PTFE frits are not considered as
a major repair.

Incorrect pump elasticity calibration.

CAUTION
Solvent compressibility calibrations acquired with a miscalibrated pump will work, but
they are not transferable to other pumps. A correct pump elasticity calibration is an
essential prerequisite for successful solvent compressibility calibrations.
➔ Calibrate the pump elasticity correctly.

1260 Infinity Binary Pump User Manual 15

1 Introduction to the Binary Pump
Overview of the Hydraulic Path

What is Solvent Compressibility Compensation?

Although the compressibility of liquids is orders of magnitude lower than
the compressibility of gases, without correction a noticeable volume error
would be seen if typical chromatographic solvents are compressed to
operating pressures as high as 600 bar. In addition, the compressibility
depends on pressure, temperature and the amount of dissolved gas. In
order to minimize the influence of the latter, the use of a vacuum
degasser is mandatory for a high flow and composition precision.
Unfortunately, the influence of the temperature on compressibility is
non- linear and cannot be calculated.
The Agilent 1260 Infinity Binary Pump features a multi point
compressibility calibration. The compressibility of a solvent is determined
at different pressures from 0 – 600 bar and stored in an XML file. This
file can be distributed to other pumps because the solvent compressibility
is independent from the pump.
The binary pump and ChemStation come with predetermined solvent
compressibility data for the most common HPLC solvents like water,
acetonitrile, methanol, etc. Users can calibrate their own solvent mixtures
with the help of an easy to use calibration procedure in the Agilent Lab
Advisor software.
Let us use the practical example from the last section once again to
understand how compressibility compensation works:
Piston 1 draws solvent at ambient pressure. The movement direction is
reversed and piston 1 now compresses the solvent until the operating
pressure of the HPLC system is reached. The outlet valve opens, and
solvent is pumped by piston 1 into pump chamber 2.
Without any compensation, the delivered volume at operating pressure
would be too low. In addition, it would take a noticeable amount of time
to recompress the solvent to operating pressure. During this time frame,
no solvent would be delivered into the system and as a result a high
pressure fluctuation (known as pressure ripple) would be observed.

16 1260 Infinity Binary Pump User Manual

 Introduction to the Binary Pump 1
Overview of the Hydraulic Path

When both solvent compressibility at the current operating pressure and

pump elasticity are known, the pump can automatically correct for the
missing volume by drawing the appropriate larger solvent volume at
ambient pressure and speed up the piston during the recompression phase
in the first pump chamber. As a result, the pump delivers the accurate
volume with any (calibrated) solvent at any pressure at a greatly reduced
pressure ripple. For applications that require lowest transition volume of
the pump, damper and mixer can be bypassed.
For compatibility with older methods from G1312A Binary Pumps, the
previous one- point compressibility compensation is available, too.
However, since the compressibility is a non- linear function, one single
compressibility value per solvent will only give good results at one
particular pressure

How Does Variable Stroke Volume Work?

The smaller the solvent volume in the pump chamber is, the faster it can
be recompressed to operating pressure. The binary pump allows to
manually or automatically adjust the pump stroke volume of the first
piston in the range of 20 – 100 µL. Due to the compression of the solvent
volume in the first pump chamber, each piston stroke of the pump will
generate a small pressure pulsation, influencing the flow ripple of the
pump. The amplitude of the pressure pulsation mainly depends on the
stroke volume and the compressibility compensation for the solvent in use.
Small stroke volumes generate less pressure pulsation than larger stroke
volumes at the same flow rate. In addition, the frequency of the pressure
pulsation will be higher. This will decrease the influence of flow
pulsations on retention times.
In gradient mode, a smaller stroke volume results in less flow ripple and
reduces the composition ripple.
The binary pump uses a processor- controlled ball screw system for driving
its pistons. The normal stroke volume is optimized for the selected flow
rate. Small flow rates use a small stroke volume while higher flow rates
use a higher stroke volume.
The stroke volume for the pump is by default set to AUTO mode. This
means that the stroke is optimized for the flow rate in use. A change to
larger stroke volumes is possible but not recommended.

1260 Infinity Binary Pump User Manual 17

1 Introduction to the Binary Pump
Leak and Waste Handling

Leak and Waste Handling

The 1200 Infinity Series has been designed for safe leak and waste
handling. It is important that all security concepts are understood and
instructions are carefully followed.

&

' 6

7

8
(

)

*

,
+
,
Figure 5 Leak and waste handling concept (overview - typical stack configuration as an
example)

18 1260 Infinity Binary Pump User Manual

 Introduction to the Binary Pump 1
Leak and Waste Handling

The solvent cabinet (1) is designed to store a maximum volume of 6 L

solvent. The maximum volume for an individual bottle stored in the
solvent cabinet should not exceed 2.5 L. For details, see the usage
guideline for the Agilent 1200 Infinity Series Solvent Cabinets (a printed
copy of the guideline has been shipped with the solvent cabinet, electronic
copies are available on the Internet).
The leak pan (2) (individually designed in each module) guides solvents to
the front of the module. The concept covers also leakages on internal
parts (e.g. the detector’s flow cell). The leak sensor in the leak pan stops
the running system as soon as the leak detection level is reached.
The leak pan's outlet port (3, A) guides excessive overfill from one module
to the next, as the solvent flows into the next module’s leak funnel (3, B)
and the connected corrugated waste tube (3, C). The corrugated waste
tube guides the solvent to the next lower positioned module’s leak tray
and sensor.
The waste tube of the sampler’s needle wash port (4) guides solvents to
waste.
The condense drain outlet of the autosampler cooler (5) guides condensate
to waste.
The waste tube of the purge valve (6) guides solvents to waste.
The waste tube connected to the leak pan outlet on each of the bottom
instruments (7) guides the solvent to a suitable waste container.

1260 Infinity Binary Pump User Manual 19

1 Introduction to the Binary Pump
Leak and Waste Handling

20 1260 Infinity Binary Pump User Manual

 1260 Infinity Binary Pump User Manual

2
Site Requirements and Specifications
Site Requirements 22
Physical Specifications 25
Performance Specifications 26

This chapter provides information about site requirements and specifications

for the binary pump.

Agilent Technologies 21
2 Site Requirements and Specifications
Site Requirements

Site Requirements

A suitable environment is important to ensure optimal performance of the

instrument.

Power Considerations
The module power supply has wide ranging capability. It accepts any line
voltage in the range described in Table 1 on page 25. Consequently there
is no voltage selector in the rear of the module. There are also no
externally accessible fuses, because automatic electronic fuses are
implemented in the power supply.

Hazard of electrical shock or damage of your instrumentation

WA R N I N G
can result, if the devices are connected to a line voltage higher than specified.
➔ Connect your instrument to the specified line voltage only.

The module is partially energized when switched off, as long as the power cord is
WA R N I N G
plugged in.
Repair work at the module can lead to personal injuries, e.g. electrical shock, when
the cover is opened and the module is connected to power.
➔ Always unplug the power cable before opening the cover.
➔ Do not connect the power cable to the instrument while the covers are removed.

Inaccessible power plug.

CAUTION
In case of emergency it must be possible to disconnect the instrument from the power
line at any time.
➔ Make sure the power connector of the instrument can be easily reached and
unplugged.
➔ Provide sufficient space behind the power socket of the instrument to unplug the
cable.

22 1260 Infinity Binary Pump User Manual

 Site Requirements and Specifications 2
Site Requirements

Power Cords
Different power cords are offered as options with the module. The female
end of all power cords is identical. It plugs into the power- input socket at
the rear. The male end of each power cord is different and designed to
match the wall socket of a particular country or region.

Absence of ground connection or use of unspecified power cord

WA R N I N G
The absence of ground connection or the use of unspecified power cord can lead to
electric shock or short circuit.
➔ Never operate your instrumentation from a power outlet that has no ground
connection.
➔ Never use a power cord other than the Agilent Technologies power cord designed
for your region.

Use of unsupplied cables

WA R N I N G
Using cables not supplied by Agilent Technologies can lead to damage of the
electronic components or personal injury.
➔ Never use cables other than the ones supplied by Agilent Technologies to ensure
proper functionality and compliance with safety or EMC regulations.

Unintended use of supplied power cords

WA R N I N G
Using power cords for unintended purposes can lead to personal injury or damage of
electronic equipment.
➔ Never use the power cords that Agilent Technologies supplies with this instrument
for any other equipment.

1260 Infinity Binary Pump User Manual 23

2 Site Requirements and Specifications
Site Requirements

Bench Space
The module dimensions and weight (see Table 1 on page 25) allow you to
place the module on almost any desk or laboratory bench. It needs an
additional 2.5 cm (1.0 inches) of space on either side and approximately
8 cm (3.1 inches) in the rear for air circulation and electric connections.
If the bench shall carry a complete HPLC system, make sure that the
bench is designed to bear the weight of all modules.
The module should be operated in a horizontal position.

Condensation
Condensation within the module
CAUTION
Condensation will damage the system electronics.
➔ Do not store, ship or use your module under conditions where temperature
fluctuations could cause condensation within the module.
➔ If your module was shipped in cold weather, leave it in its box and allow it to warm
slowly to room temperature to avoid condensation.

24 1260 Infinity Binary Pump User Manual

 Site Requirements and Specifications 2
Physical Specifications

Physical Specifications

Table 1 Physical Specifications

Type Specification Comments

Weight 15.5 kg (34 lbs)

Dimensions 180 x 345 x 435 mm

(height × width × depth) (7 x 13.5 x 17 inches)

Line voltage 100 – 240 VAC, ± 10 % Wide-ranging

capability

Line frequency 50 or 60 Hz, ± 5 %

Power consumption 220 VA, 74 W / 253 BTU Maximum

Ambient operating 0–55 °C (32–131 °F)

temperature

Ambient non-operating -40 – 70 °C (-40 – 158 °F)

temperature

Humidity < 95 % r.h. at 40 °C (104 °F) Non-condensing

Operating altitude Up to 2000 m (6562 ft)

Non-operating altitude Up to 4600 m (15091 ft) For storing the module

Safety standards: Installation category II, Pollution degree 2 For indoor use only.
IEC, CSA, UL

1260 Infinity Binary Pump User Manual 25

2 Site Requirements and Specifications
Performance Specifications

Performance Specifications

Table 2 Performance Specifications of the Agilent 1260 Infinity Binary Pump (G1312B)
Type Specification Comments
Hydraulic system Two dual piston in series pumps with
servo-controlled variable stroke drive,
power transmission by gears and ball
screws, floating pistons
Setable flow range Set points 0.001 – 5 mL/min, in
0.001 mL/min increments
Flow range 0.05 – 5.0 mL/min
Flow precision ≤0.07 % RSD or ≤0.02 min SD, whatever is based on retention time at
greater constant room temperature
Flow accuracy ± 1 % or 10 µL/min, what ever is greater pumping degassed H2O at
10 MPa (100 bar)
Pressure operating Operating range 0 – 60 MPa (0 – 600 bar,
range 0 – 8700 psi) up to 5 mL/min
Pressure pulsation < 2 % amplitude (typically < 1.3 %), or
< 0.3 MPa (3 bar), whatever is greater, at
1 mL/min isopropanol, at all pressures
> 1 MPa (10 bar, 147 psi)
Low delay volume configuration:
< 5 % amplitude (typically < 2 %)
Compressibility Pre-defined, based on mobile phase
compensation compressibility
Recommended pH 1.0 – 12.5 , solvents with pH < 2.3 should
range not contain acids which attack stainless
steel
Gradient formation High-pressure binary mixing
Delay volume Standard delay volume configuration: measured with water at
600 – 800 µL, (includes 400 µL mixer), 1 mL/min (water/caffeine
dependent on back pressure tracer)
Low delay volume configuration:
120 µL

26 1260 Infinity Binary Pump User Manual

 Site Requirements and Specifications 2
Performance Specifications

Table 2 Performance Specifications of the Agilent 1260 Infinity Binary Pump (G1312B)
Type Specification Comments
Composition range settable range: 0 – 100 %
recommended range: 1 – 99 % or
5 µL/min per channel, whatever is greater
Composition < 0.15 % RSD or < 0.04 min SD whatever at 0.2 and 1 mL/min; based
precision is greater on retention time at constant
room temperature
Composition ± 0.35 % absolute, at 2 mL/min, at (water/caffeine tracer)
accuracy 10 MPa (100 bar)
Control Agilent control software (e.g.
ChemStation, EZChrom, OL, MassHunter)
Local control Agilent Instant Pilot Revision B.02.00 or above
Analog output For pressure monitoring, 1.33 mV/bar,
one output
Communications Controller-area network (CAN), RS-232C,
APG Remote: ready, start, stop and
shut-down signals, LAN optional
Safety and Extensive support for troubleshooting and
maintenance maintenance is provided by the Instant
Pilot, Agilent Lab Advisor, and the
Chromatography Data System.
Safety-related features are leak detection,
safe leak handling, leak output signal for
shutdown of pumping system, and low
voltages in major maintenance areas.
GLP features Early maintenance feedback (EMF) for
continuous tracking of instrument usage
in terms of seal wear and volume of
pumped mobile phase with pre-defined
and user settable limits and feedback
messages. Electronic records of
maintenance and errors
Housing All materials are recyclable

For use with flow rates below 500 µl/min or for use without damper and mixer a vacuum
NOTE degasser is required.
All specification measurements are done with degassed solvents.

1260 Infinity Binary Pump User Manual 27

2 Site Requirements and Specifications
Performance Specifications

28 1260 Infinity Binary Pump User Manual

 1260 Infinity Binary Pump User Manual

3
Installing the Pump
Unpacking the Binary Pump 30
Optimizing the Stack Configuration 33
Installation Information on Leak and Waste Handling 41
Installing the Binary Pump 45
Flow Connections with Solvent Selection Valve 48
Flow Connections without Solvent Selection Valve 51
Priming the System 54
Initial Priming 54
Regular Priming 56
Changing Solvents 57

This chapter gives information about the preferred stack setup for your system
and the installation of your binary pump.

Agilent Technologies 29
3 Installing the Pump
Unpacking the Binary Pump

Unpacking the Binary Pump

Damaged Packaging
If the delivery packaging shows signs of external damage, please call your
Agilent Technologies sales and service office immediately. Inform your
service representative that the instrument may have been damaged during
shipment.

"Defective on arrival" problems

CAUTION
If there are signs of damage, please do not attempt to install the module. Inspection by
Agilent is required to evaluate if the instrument is in good condition or damaged.
➔ Notify your Agilent sales and service office about the damage.
➔ An Agilent service representative will inspect the instrument at your site and
initiate appropriate actions.

30 1260 Infinity Binary Pump User Manual

 Installing the Pump 3
Unpacking the Binary Pump

Delivery Checklist
Compare the delivery checklist with the contents of the shipping boxes to
ensure completeness of the shipment. The contents lists is shown below.
For parts identification check the illustrated parts breakdown in “Parts
and Materials for Maintenance” on page 183. Please report missing or
damaged parts to your local Agilent Technologies sales and service office.

p/n Description
G1312B Agilent 1260 Infinity Binary Pump
optionally with active seal wash and/or solvent selection valve
G1311-60003 (2x) Bottle-head assembly (OPTIONAL)
G1369C Interface board (LAN) (OPTIONAL)
G1312-67500 Calibration capillary assembly
G1312-90303 Agilent 1260 Infinity Binary LC System User Guide
not orderable
G4201-68707 HPLC Starter Kit incl. 0.17 mm i.d. cap (OPTIONAL)
G4202-68707 HPLC Starter Kit incl. 0.12 mm i.d. cap (OPTIONAL)
G4203-68708 HPLC System Tool Kit (OPTIONAL)
827975-902 Column: SB-C18, 4.6x50 mm, 1.8 µm, 600 bar (OPTIONAL)
959961-902 Column Eclipse Plus C18, 4.6 x 100 mm, 3.5 µm (OPTIONAL)
699975-302 Column Poroshell 120 EC-C18, 3.0 x 50 mm, 2.7 µm (OPTIONAL)
699975-902 Column Poroshell 120 EC-C18, 4.6 x 50 mm, 2.7 µm (OPTIONAL)
5067-4770 Solvent Cabinet Kit (OPTIONAL)
G4800-64500 Agilent 1200 Infinity Series User Documentation DVD (OPTIONAL)
not orderable (OPTIONAL)
M8500A Lab Advisor incl. license (OPTIONAL)
Power cord

Items identified as "optional" are additional accessories. They are not included in the
NOTE standard scope of delivery.

Items identified as "not orderable" can be downloaded from the Agilent website
NOTE http://www.agilent.com.

1260 Infinity Binary Pump User Manual 31

3 Installing the Pump
Unpacking the Binary Pump

Accessory Kit
The Accessory Kit (G1311- 68755) contains the following items:

p/n Description

5062-2461 Waste tube, 5 m (reorder pack)

5063-6527 Tubing assembly, i.d. 6 mm, o.d. 9 mm, 1.2 m (to waste)

5181-1519 CAN cable, Agilent module to module, 1 m

G1329-87300 Capillary ST 0.17 mm x 900 mm S/S

pump to thermostatted autosampler

G1312-87303 Capillary ST 0.17 mm x 400 mm S/S

pump to injector

5042-9967 Tubing clip (set of 5 clips)

32 1260 Infinity Binary Pump User Manual

 Installing the Pump 3
Optimizing the Stack Configuration

Optimizing the Stack Configuration

Agilent 1260 Infinity Binary LC in Standard Delay Volume

Configuration
This configuration is typically used when using 4.6 mm and 3.0 mm i.d.
columns. It is optimized for high flow rates and maximum sensitivity.
For a more detailed help on configuring your instrument, refer to the
Agilent 1260 Infinity Binary LC System User Guide.

1260 Infinity Binary Pump User Manual 33

3 Installing the Pump
Optimizing the Stack Configuration

9Z\VhhZg

Ejbe
)%%bbm%#&,bb

=^E6AH
%#&,bbhZVi

&%*bbm%#&,bb

8ddaZg[dg6AH

I88 &%*bbm%#&,bb
OZgd9ZVYKdajbZjc^dc

&(¥aXZaa
969

Figure 6 Agilent 1260 Infinity Binary LC in standard delay volume configuration

for 4.6 mm and 3.0 mm i.d. columns

34 1260 Infinity Binary Pump User Manual

 Installing the Pump 3
Optimizing the Stack Configuration

Agilent 1260 Infinity Binary LC in Medium Delay Volume Configuration

This setup is used for best signal to noise ratio using 2.1 mm and 3.0 mm
columns.
For a more detailed help on configuring your instrument, refer to the
Agilent 1260 Infinity Binary LC System User Guide.

9Z\VhhZg

Ejbe )%%bbm%#&,bb

=^E6AH
%#&'bbhZVi XV#('%bbm%#&'bb

8ddaZg[dg6AH CZlAdl9ZaVnKdajbZ
=ZVi":mX]Vc\Zg

CZlA9KedhiXdajbcXddaZg
I88 %#&'bb>9XVe#

'¥aXZaa

969

Figure 7 Agilent 1260 Infinity Binary LC in low delay volume configuration

for 2.1 mm and 3.0 mm id columns

1260 Infinity Binary Pump User Manual 35

3 Installing the Pump
Optimizing the Stack Configuration

Figure 8 Binary pump in medium delay volume configuration

36 1260 Infinity Binary Pump User Manual

 Installing the Pump 3
Optimizing the Stack Configuration

Agilent 1260 Infinity Binary LC in Low Delay Volume Configuration

In this configuration the LC is optimized for speed with the 2.1 mm
columns.
For a more detailed help on configuring your instrument, refer to the
Agilent 1260 Infinity Binary LC System User Guide.

9Z\VhhZg

Ejbe )%%bbm%#&,bb

=^E6AH
%#&'bbhZVi XV#('%bbm%#&'bb

8ddaZg[dg6AH CZlAdl9ZaVnKdajbZ
=ZVi":mX]Vc\Zg

CZlA9KedhiXdajbcXddaZg
I88 %#&'bb>9XVe#

'¥aXZaa

969

Figure 9 Agilent 1260 Infinity Binary LC in low delay volume configuration

for 2.1 mm and 3.0 mm id columns

1260 Infinity Binary Pump User Manual 37

3 Installing the Pump
Optimizing the Stack Configuration

Agilent 1260 Infinity Binary LC in Low Delay Volume Configuration

with Post Column Cooler
This configuration is usually used for short 2.1 mm and 3.0 mm columns
optimized for high flow rates.
For a more detailed help on configuring your instrument, refer to the
Agilent 1260 Infinity Binary LC System User Guide.

38 1260 Infinity Binary Pump User Manual

 Installing the Pump 3
Optimizing the Stack Configuration

9Z\VhhZg

Ejbe )%%bbm%#&,bb

=^E6AH
%#&'bbhZVi XV#('%bbm%#&'bb

8ddaZg[dg6AH CZlAdl9ZaVnKdajbZ
=ZVi":mX]Vc\Zg

CZlA9KedhiXdajbcXddaZg
I88 %#&'bb>9XVe#

'¥aXZaa

969

Figure 10 Agilent 1260 Infinity Binary LC in low delay volume configuration

for 2.1 mm and 3.0 mm id columns

1260 Infinity Binary Pump User Manual 39

3 Installing the Pump
Optimizing the Stack Configuration

Agilent 1260 Infinity Binary LC in Low Delay Volume Configuration

with Automated Column Regeneration and MS
This is the recommended setup to achieve minimum cycle time using MS
detection.
For a more detailed help on configuring your instrument, refer to the
Agilent 1260 Infinity Binary LC System User Guide.

New LDV Heat - X

DAD or MWD 2 μl cell

New LDV
post column cooler
0.12 mm ID cap.
TCC
Degasser
SQ-MSD

Reg. Pump

WPS
0.12 mm seat
Pump
Cooler f. WPS

650 mm x 0.17 mm ca. 320 mm x 0.12 mm

Figure 11 Agilent 1260 Infinity Binary LC with Automated column regeneration and MS
detection in low delay volume configuration

40 1260 Infinity Binary Pump User Manual

 Installing the Pump 3
Installation Information on Leak and Waste Handling

Installation Information on Leak and Waste Handling

The Agilent 1200 Infinity Series has been designed for safe leak and waste
handling. It is important that all security concepts are understood and
instructions are carefully followed.

Toxic, flammable and hazardous solvents, samples and reagents

WA R N I N G
The handling of solvents, samples and reagents can hold health and safety risks.
➔ When working with these substances observe appropriate safety procedures (for
example by wearing goggles, safety gloves and protective clothing) as described in
the material handling and safety data sheet supplied by the vendor, and follow good
laboratory practice.
➔ The volume of substances should be reduced to the minimum required for the
analysis.
➔ Never exceed the maximal permissible volume of solvents (6 L) in the solvent
cabinet.
➔ Do not use bottles that exceed the maximum permissible volume as specified in the
usage guideline for the Agilent 1200 Infinity Series Solvent Cabinets.
➔ Arrange the bottles as specified in the usage guideline for the solvent cabinet.
➔ A printed copy of the guideline has been shipped with the solvent cabinet,
electronic copies are available on the Internet.

Recommendations for Solvent Cabinet

NOTE
For details, see the usage guideline for the Agilent 1200 Infinity Series Solvent Cabinets.

1260 Infinity Binary Pump User Manual 41

3 Installing the Pump
Installation Information on Leak and Waste Handling

&

' 6

7

8
(

)

*

,
+
,
Figure 12 Leak and waste handling (overview - typical stack configuration as an
example)

42 1260 Infinity Binary Pump User Manual

 Installing the Pump 3
Installation Information on Leak and Waste Handling

1 Solvent cabinet

2 Leak pan

3 Leak pan's outlet port (A), leak funnel (B) and corrugated waste tube (C)

4 Waste tube of the sampler’s needle wash

5 Condense drain outlet of the autosampler cooler

6 Waste tube of the purge valve

7 Waste tube

1 Stack the modules according to the adequate stack configuration.

The leak pan outlet of the upper module must be vertically positioned
above the leak tray of the lower module, see Figure 12 on page 42.
2 Connect data and power cables to the modules, see section Installing
the Module below.
3 Connect capillaries and tubes to the modules, see section Flow
Connections to the module below or the relevant system manual.

Toxic, flammable and hazardous solvents, samples and reagents

WA R N I N G
➔ Keep solvent path free from blockages.
➔ Keep the flow path closed (in case the pump in the system is equipped with a
passive inlet valve, solvent may leak out due to hydrostatic pressure, even if your
instrument is off).
➔ Avoid loops.
➔ Tubes must not sag.
➔ Do not bend tubes.
➔ Do not immerse tube end in waste liquid.
➔ Do not intubate tubes in other tubes.
➔ For correct tubing follow instructions on label attached to the module.

1260 Infinity Binary Pump User Manual 43

3 Installing the Pump
Installation Information on Leak and Waste Handling

Figure 13 Warning label (illustration for correct waste tubing)

44 1260 Infinity Binary Pump User Manual

 Installing the Pump 3
Installing the Binary Pump

Installing the Binary Pump

Parts required # p/n Description

1 Pump
1 Data System
and/or
1 G4208A Instant Pilot
1 Power cord

For other cables see text below and “Overview” on page 202.

Preparations • Locate bench space.

• Provide power connections.
• Unpack the module.

Module is partially energized when switched off, as long as the power cord is
WA R N I N G
plugged in.
Repair work at the module can lead to personal injuries, e.g. shock hazard, when the
cover is opened and the module is connected to power.
➔ Make sure that it is always possible to access the power plug.
➔ Remove the power cable from the instrument before opening the cover.
➔ Do not connect the power cable to the Instrument while the covers are removed.

"Defective on arrival" problems

CAUTION
If there are signs of damage, please do not attempt to install the module. Inspection by
Agilent is required to evaluate if the instrument is in good condition or damaged.
➔ Notify your Agilent sales and service office about the damage.
➔ An Agilent service representative will inspect the instrument at your site and
initiate appropriate actions.

1260 Infinity Binary Pump User Manual 45

3 Installing the Pump
Installing the Binary Pump

1 Place the module on the bench in a horizontal position.

2 Ensure the power switch on the front of the pump is OFF (switch
stands out).

HiVijh>cY^XVidg

EdlZghl^iX]

HZg^VacjbWZg

Figure 14 Front of Binary Pump

3 Connect the power cable to the power connector at the rear of the
module.

46 1260 Infinity Binary Pump User Manual

 Installing the Pump 3
Installing the Binary Pump

4 Connect the required interface cables to the rear of the module.

Configuration switch

Slot for interface board

RS232

Remote

Analog output

CAN-Bus
HP-IB

Power plug

Figure 15 Rear of the Binary Pump

5 Connect the capillary, solvent tubes and waste tubings (see “Flow
Connections with Solvent Selection Valve” on page 48 or “Flow
Connections without Solvent Selection Valve” on page 51).
6 Press the power switch to turn on the module.

The power switch stays pressed in and a green indicator lamp in the power switch is on
NOTE when the module is turned on. When the line power switch stands out and the green light
is off, the module is turned off.

7 Purge the pump (see “Initial Priming” on page 54).

The pump is shipped with default configuration settings. To change
these settings, refer to “Setting the 8- bit Configuration Switch (without
On- board) LAN” on page 228.

1260 Infinity Binary Pump User Manual 47

3 Installing the Pump
Flow Connections with Solvent Selection Valve

Flow Connections with Solvent Selection Valve

Parts required # p/n Description

1 Other modules
1 G1311-68755 Accessory Kit
2 wrenches 1/4 - 5/16 inch for capillary connections

Preparations Pump is installed in the LC system

When opening capillary or tube fittings, solvents may leak out.

WA R N I N G
The handling of toxic and hazardous solvents and reagents can carry health risks.
➔ Observe appropriate safety procedures (for example, wear goggles, safety gloves
and protective clothing) as described in the material handling and safety data sheet
supplied by the solvent vendor, especially when toxic or hazardous solvents are
used.

1 Remove the front cover by pressing the snap fasteners on both sides.

Figure 16 Removing the Front Cover

48 1260 Infinity Binary Pump User Manual

 Installing the Pump 3
Flow Connections with Solvent Selection Valve

2 If available, place the online degasser on top of the pump.

3 Place the solvent cabinet on top of the module.
4 Set the four bottles into the solvent cabinet and screw a bottle head
assembly onto each bottle.
5 Connect the solvent tubes from the bottle head assemblies to the inlet
connectors A1, A2, B1 and B2 of the solvent selection valve. Make sure
to use the brown bottle for the aqueous solvent (usually channel A1).
6 Label the tubes accordingly using the supplied stickers and fix the
tubes in the clips of solvent cabinet and binary pump.
7 Hold the waste tubing with a piece of sandpaper and push it onto the
purge valve outlet. Place the end into your waste system.
8 If the pump is not part of an Agilent 1260 Infinity system stack or
placed on the bottom of a stack, connect the waste tube to the waste
outlet of the pump leak handling system.
9 Connect the pump outlet capillary (pump to injection device) to the
outlet of the purge valve.

1260 Infinity Binary Pump User Manual 49

3 Installing the Pump
Flow Connections with Solvent Selection Valve

10 Purge your system prior to the first use (see “Initial Priming” on
page 54).

7diiaZ"]ZVYVhhZbWan

HdakZciXVW^cZi

9Z\VhhZg

8]VccZa6& 8]VccZa6'

EgZhhjgZhZchdg HiVi^Xb^mZg

Ejg\ZkVakZ

Ejbe]ZVY6 Ejbe]ZVY7

6Xi^kZ^caZikVakZ6 6Xi^kZ^caZikVakZ7
AZV`YgV^c
LVhiZijW^c\

DjiaZiXVe^aaVgnidVjidhVbeaZg 8]VccZa7'
8]VccZa7& HdakZcihZaZXi^dckVakZ

Figure 17 Binary Pump with Solvent Selection Valve

50 1260 Infinity Binary Pump User Manual

 Installing the Pump 3
Flow Connections without Solvent Selection Valve

Flow Connections without Solvent Selection Valve

Parts required # p/n Description

1 Other modules
1 G1311-68755 Accessory Kit
2 wrenches 1/4 - 5/16 inch for capillary connections

Preparations Pump is installed in the LC system

When opening capillary or tube fittings, solvents may leak out.

WA R N I N G
The handling of toxic and hazardous solvents and reagents can carry health risks.
➔ Observe appropriate safety procedures (for example, wear goggles, safety gloves
and protective clothing) as described in the material handling and safety data sheet
supplied by the solvent vendor, especially when toxic or hazardous solvents are
used.

1 Remove the front cover by pressing the snap fasteners on both sides.

Figure 18 Removing the Front Cover

1260 Infinity Binary Pump User Manual 51

3 Installing the Pump
Flow Connections without Solvent Selection Valve

2 Place the solvent cabinet on top of the module.

3 Place the bottles into the solvent cabinet and place a bottle head
assembly into each bottle.
4 Connect the solvent tubes from the bottle head assemblies to the inlet
adapters of the active inlet valves. Fix the tubes in the clips of solvent
cabinet and binary pump.
5 Hold the waste tubing with a piece of sandpaper and push it onto the
purge valve outlet. Place the end into your waste system.
6 If the pump is not part of an Agilent 1260 Infinity system stack or
placed on the bottom of a stack, connect the waste tube to the waste
outlet of the pump leak handling system.
7 Connect the pump outlet capillary (pump to injection device) to the
outlet of the purge valve.

52 1260 Infinity Binary Pump User Manual

 Installing the Pump 3
Flow Connections without Solvent Selection Valve

8 Purge your system before first use (see “Initial Priming” on page 54).

7diiaZ"]ZVYVhhZbWan

HdakZciXVW^cZi

9Z\VhhZg

EgZhhjgZhZchdg

HiVi^Xb^mZg

Ejg\ZkVakZ

Ejbe]ZVY6 Ejbe]ZVY7
6YVeiZg

6Xi^kZ^caZikVakZ6 6Xi^kZ^caZikVakZ7

LVhiZijW^c\
LVhiZdjiaZi
DjiaZiXVe^aaVgnidVjidhVbeaZg

Figure 19 Flow Connection of Binary Pump without Solvent Selection Valve

1260 Infinity Binary Pump User Manual 53

3 Installing the Pump
Priming the System

Priming the System

Initial Priming

When Before a degasser or solvent tubing can be used, it is necessary to prime the system. Isopropanol is
recommended as priming solvent due to its miscibility with nearly all HPLC solvents and its
excellent wetting properties.

Parts required # Description

1 Isopropanol

Preparations Connect all modules hydraulically as described in the respective module manuals.
Fill each solvent bottle with 100 mL isopropanol
Switch the system on

When opening capillary or tube fittings, solvents may leak out.

WA R N I N G
The handling of toxic and hazardous solvents and reagents can carry health risks.
➔ Observe appropriate safety procedures (for example, wear goggles, safety gloves
and protective clothing) as described in the material handling and safety data sheet
supplied by the solvent vendor, especially when toxic or hazardous solvents are
used.

The purge tool of the Lab Advisor can be used to purge the pump automatically.
NOTE

If the pump is not able to draw in the solvent from the bottles, use a syringe to move the
NOTE solvent manually through tubing and degasser.

When priming the vacuum degasser with a syringe, the solvent is drawn through the
NOTE degasser tubes very quickly. The solvent at the degasser outlet will therefore not be fully
degassed. Pump for approximately 10 minutes at your desired flow rate before starting an
analysis. This will allow the vacuum degasser to properly degas the solvent in the degasser
tubes.

54 1260 Infinity Binary Pump User Manual

 Installing the Pump 3
Priming the System

1 Open the purge valve of the pump

2 Set the flow rate to 5 mL/min.
3 Select channel A1
4 Turn the flow on
5 Observe if the solvent in the tubing of channel A1 is advancing towards
the pump. If it isn’t, disconnect the solvent tubing from the solvent
selection valve, attach a syringe with a syringe adapter and pull the
liquid through the degasser. Reattach the tubing to the solvent selection
valve.
6 Pump 30 mL isopropanol to remove residual air bubbles.
7 Switch to the next solvent channel and repeat steps 5 and 6 until all
channels have been purged.
8 Turn the flow off and close the purge valve.

1260 Infinity Binary Pump User Manual 55

3 Installing the Pump
Priming the System

Regular Priming

When When the pumping system has been turned off for a certain time (for example, overnight) air will
rediffuse into the solvent channel between the vacuum degasser and the pump. If solvents
containing volatile components are left in the degasser without flow for a prolonged period, there
will be a slight loss of the volatile components.

Preparations Switch the system on

The purge tool of the Lab Advisor can be used for automatically purging the pump.
NOTE

1 Open the purge valve of your pump by turning it counterclockwise and

set the flow rate to 5 mL/min.
2 Flush the vacuum degasser and all tubes with at least 10 mL of solvent.
3 Repeat step 1 and 2 for the other channel(s) of the pump.
4 Set the required composition and flow rate for your application and
close the purge valve.
5 Pump for approximately 10 minutes before starting your application.

56 1260 Infinity Binary Pump User Manual

 Installing the Pump 3
Priming the System

Changing Solvents

When When the solvent of a channel is to be replaced by another solvent that is not compatible (solvents
are immiscible or one solvent contains a buffer), it is necessary to follow the procedure below to
prevent clogging of the pump by salt precipitation or residual liquid droplets in parts of the system.

Parts required # p/n Description

1 Purging solvent(s), see Table 3 on page 58
1 5022-2184 Union ZDV

Preparations Remove the column and replace it by a ZDV fitting

Prepare bottles with appropriate intermediate solvents (see Table 3 on page 58)

1 If the channel is not filled with buffer, proceed to step 4.

2 Place the solvent intake filter into a bottle of water.
3 Flush the channel at a flow rate suitable for the installed tubing
(typically 3 – 5 mL/min) for 10 min.
4 Modify the flow path of your system as required for your application.
For delay volume optimization, see the Binary LC System User Guide.
Buffer salt of aqueous buffers may precipitate in residual isopropanol.
CAUTION
Capillaries and filter may be clogged by precipitating salt.
➔ Flush solvent lines containing high concentration of salts first with water before
introducing organic solvent.
➔ Do not perform steps 5 to 7 for channels running with aqueous buffer as solvent.

5 Replace the solvent bottle by a bottle of isopropanol.

6 Flush the channel at a flow rate suitable for the installed tubing
(typically 3 – 5 mL/min) for 5 min.
7 Swap the bottle of isopropanol with a bottle of solvent for your
application.
8 Repeat steps 1 to 7 for the other channel(s) of the pump.
9 Install the desired column, set the required composition and flow rate
for your application and equilibrate the system for approx. 10 minutes
prior to starting a run.

1260 Infinity Binary Pump User Manual 57

3 Installing the Pump
Priming the System

Table 3 Choice of Priming Solvents for Different Purposes

Activity Solvent Comments

After an installation Isopropanol Best solvent to flush air out of

the system
When switching between Isopropanol Miscible with almost all
reverse phase and normal solvents
phase (both times)

After an installation Ethanol or methanol Alternative to isopropanol

(second choice) if no
isopropanol is available

To clean the system when HPLC grade water Best solvent to re-dissolve
using buffers buffer crystals
After changing aqueous HPLC grade water Best solvent to re-dissolve
solvents buffer crystals

After the installation of normal Hexane + 5 % isopropanol Good wetting properties

phase seals (PE seals (pack of
2) (0905-1420))

58 1260 Infinity Binary Pump User Manual

 1260 Infinity Binary Pump User Manual

4
Using the Pump
Leak and Waste Handling 60
Hints for Successful Use of the Binary Pump 61
Setting up the Pump with the G4208A Instant Pilot 62
Setting up the Agilent 1260 Infinity Binary Pump G1312B with the
Instrument Control Interface 63
Overview 63
Setup of Basic Pump Parameters 64
Pump Control 65
Auxiliary Pump Parameters 66
Data Curves 67
Bottle Filling 67
Solvent Information 69
Algae Growth in HPLC Systems 74
How to Prevent and/or Reduce the Algae Problem 75
Prevent Blocking of Solvent Filters 76
Checking the Solvent Filters 77
Cleaning the Solvent Filters 77

This chapter explains the operational parameters of the binary pump.

Agilent Technologies 59
4 Using the Pump
Leak and Waste Handling

Leak and Waste Handling

Toxic, flammable and hazardous solvents, samples and reagents

WA R N I N G
The handling of solvents, samples and reagents can hold health and safety risks.
➔ When working with these substances observe appropriate safety procedures (for
example by wearing goggles, safety gloves and protective clothing) as described in
the material handling and safety data sheet supplied by the vendor, and follow good
laboratory practice.
➔ The volume of substances should be reduced to the minimum required for the
analysis.
➔ Do not operate the instrument in an explosive atmosphere.
➔ Never exceed the maximal permissible volume of solvents (6 L) in the solvent
cabinet.
➔ Do not use bottles that exceed the maximum permissible volume as specified in the
usage guideline for the Agilent 1200 Infinity Series Solvent Cabinets.
➔ Arrange the bottles as specified in the usage guideline for the solvent cabinet.
➔ A printed copy of the guideline has been shipped with the solvent cabinet,
electronic copies are available on the Internet.
➔ The residual free volume in the appropriate waste container must be large enough
to collect the waste liquid.
➔ Check the filling level of the waste container regularly.
➔ To achieve maximal safety, check the correct installation regularly.

Recommendations for Solvent Cabinet

NOTE
For details, see the usage guideline for the Agilent 1200 Infinity Series Solvent Cabinets.

For details on correct installation, see “Installation Information on Leak

and Waste Handling” on page 41.

60 1260 Infinity Binary Pump User Manual

 Using the Pump 4
Hints for Successful Use of the Binary Pump

Hints for Successful Use of the Binary Pump

• Place solvent cabinet with the solvent bottles always on top (or at a
higher level) of the pump.
• When using the binary pump without vacuum degasser, shortly degas
your solvents by putting the solvent to a suitable container and
applying a vacuum pressure for some time. If possible apply solvent
conditions that will decrease the gas solubility over time (for example,
warming up the solvents).
• The use of a vacuum degasser is mandatory for flow rates below
0.5 mL/min and for configurations without damper and mixer.
• When using the binary pump with vacuum degasser, flush the degasser
with at least 5 mL per channel before operating the pump, especially
when the pumping system had been turned off for a certain length of
time (for example, overnight) and volatile solvent mixtures are used in
the channels (see “Regular Priming” on page 56).
• Prevent blocking of solvent inlet filters (never use the pump without
solvent inlet filters). Growth of algae should be avoided (see “Prevent
Blocking of Solvent Filters” on page 76).
• Check purge valve frit and column frit in regular time intervals. A
blocked purge valve frit can be identified by black, yellow or greenish
layers on its surface or by a pressure greater than 10 bar in low delay
volume configuration and 20 bar in standard configuration when
pumping distilled water at a rate of 5 mL/min with an open purge
valve.
• Whenever possible use a minimum flow rate of 5 µL/min per solvent
channel to avoid crossflow of solvent into the unused pump channel.
• Whenever exchanging the pump seals, the purge valve frit should be
exchanged, too.
• When using buffer solutions, flush the system with water before
switching it off. The seal wash option should be used when buffer
solutions with concentrations of 0.1 M or higher are being pumped for
long periods of time.
• Check the pump pistons for scratches, grooves and dents when
changing the piston seals. Damaged pistons cause micro leaks and will
decrease the lifetime of the seals.
• After changing the piston seals, apply the seal wear- in procedure (see
“Seal Wear- in Procedure” on page 169).
• Place the aqueous solvent on channel A and the organic solvent on
channel B. The default compressibility settings are set accordingly.

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4 Using the Pump
Setting up the Pump with the G4208A Instant Pilot

Setting up the Pump with the G4208A Instant Pilot

Generic operation of the G4208A Instant Pilot is covered in the Agilent

Instant Pilot G4208A User's Guide (G4208- 90006). Details about setting up
module specific parameters can be found in the Instant Pilot online help.
The pump parameters are described in detail in “Overview” on page 63.

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 Using the Pump 4
Setting up the Agilent 1260 Infinity Binary Pump G1312B with the Instrument Control Interface

Setting up the Agilent 1260 Infinity Binary Pump G1312B with the
Instrument Control Interface

Overview
Parameters described in following sections is offered by the instrument
control interface and can usually be accessed through Agilent instrument
control software. For details, please refer to manuals and online help of
respective user interfaces.

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4 Using the Pump
Setting up the Agilent 1260 Infinity Binary Pump G1312B with the Instrument Control Interface

Setup of Basic Pump Parameters

The most important parameters of the pump are listed in Table 4 on
page 64.

Table 4 Basic pump parameters

Parameter Limits Description

• Flow 0.001 – 5 mL/min Total flow rate of the pump. See “When to Remove Damper and Mixer” on
page 84 for pump hardware modifications to achieve lowest delay volume.

• Stop Time 0.01 min - no limit The stop time of the pump usually controls the run time of the whole LC system.
Use no limit to stop the run manually (useful for method development).

• Post Time off - 99999 min Time between the end of a run and the start of the next. Used for column
equilibration after a gradient.

• Pressure Max: 0 – 600 bar Max must be bigger than Min! Set max pressure to the maximum operating
Limits Min: 0 – 600 bar pressure of your column. A min pressure setting of e.g. 10 bar will turn off your
pump automatically when running out of solvent. A smarter way, however, is to
use the bottle fillings function (see “Bottle Filling” on page 67).

• Solvent A 0 – 100 % Although channel A can be set to 0 %, it cannot be turned off. This channel should
be used for the aqueous phase (water).

• Solvent B off - 100 % The percentage of channel B is automatically complemented by channel A to give
100 %.

• Solvent H2O, ACN, MeOH, IPA Select the solvent you are using in the respective solvent channel from the
type drop-down list. In case your solvent is not listed, perform a solvent compressibility
calibration (see “Running the Solvent Compressibility Calibration” on page 134.
For details on solvent compressibility see “Binary Pump Solvent Compressibility
Calibration” on page 133.

• Solvent Free text field for a description of the solvent. This description will show up in
Comment method printouts, etc.

• Timetable max. number of lines Use the timetable to build solvent gradients, flow gradients, or combinations of
depends on free both. Gradients are always linear. Use multiple timetable entries to mimic
space in pump exponential or parabolic gradients.
memory
• Display There are three ways to display the timetable:
• in tabular form
• as flow/pressure graph
• as solvent percentage plot
Values can only be changed in tabular view.

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 Using the Pump 4
Setting up the Agilent 1260 Infinity Binary Pump G1312B with the Instrument Control Interface

Pump Control
The pump can be switched between following states: On, Off or to Standby.
In Standby, the pump motor is still controlled. When the pump is switched
on from standby, it does not re- initialize.

Upon initialization, the pump ignores the Maximum Flow Gradient value.
CAUTION
This can result in a rapid and uncontrolled pressure increase.
➔ To prevent harm to the column, open the purge valve until the initialization is
finished.

The optional seal wash pump can be controlled by either switching it off,
using it for a single time or specifying frequency and duration of periodic
wash intervals.

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4 Using the Pump
Setting up the Agilent 1260 Infinity Binary Pump G1312B with the Instrument Control Interface

Auxiliary Pump Parameters

The auxiliary pump parameters are pre- set to fit most applications.
Adjustments should only be made when required. Table 5 on page 66
shows the available auxiliary parameters with their default values.

Upon initialization, the pump ignores the Maximum Flow Gradient value.
CAUTION
This can result in a rapid and uncontrolled pressure increase.
➔ To prevent harm to the column, open the purge valve until the initialization is
finished.

Table 5 Auxiliary pump parameters

Parameter Limits Description

• Maximum Flow 0.1 – 100 mL/min² With this parameter flow rate changes can be ramped up and down slowly to
Gradient default: avoid pressure shocks to the column. The default value is 100 mL/min² which
100 mL/min² in fact turns the function off.

• Minimum 20 – 100 µL The volume one pump piston delivers per stroke. In general, a smaller stroke
Stroke default: Auto volume results in lower pump ripple. The Auto setting adjusts the strokes
dynamically to the lowest possible value.
The strokes can be set individually for pump heads A and B.

• Compressibility 0 - 150·10-6 /bar For best performance, check option Use enhanced compressibility calibration.
bar or enhanced With this option, the pump will use solvent data libraries provided by Agilent or
compressibility data generated by using solvent compressibility calibrations (see “Running the
calibration Solvent Compressibility Calibration” on page 134 ).
default: use For details on solvent compressibility see “Binary Pump Solvent
enhanced comp. Compressibility Calibration” on page 133.
calibration For backward compatibility to 400 bar pumps, the solvent compressibility can
still be set manually for each channel when the box is unticked.

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 Using the Pump 4
Setting up the Agilent 1260 Infinity Binary Pump G1312B with the Instrument Control Interface

Data Curves
The binary pump provides the possibility to store the following operational
data in the data file of the Agilent data system:
• Solvent percentage for each channel,
• pump flow,
• pressure

The pressure data curve is generated from the pressure sensor readings, while %A, %B and
NOTE flow are calculated from the method settings of the pump.

For details, please refer to the online help or manual of your instrument
control software.

Bottle Filling
The pump offers a powerful feature to monitor the liquid level in the
solvent bottles. With total bottle volume and initial filling volume set
correctly, the pump subtracts the consumed volume continuously from the
initial value and stops the pump and method/sequence execution before
the system runs dry or an analysis is corrupted.

The bottle filling feature fails if multiple solvent inlets are put into one solvent bottle!
CAUTION
➔ In that case implement a minimum pressure limit (see Table 4 on page 64) to avoid
that the pump runs dry when solvents are empty.

Table 6 on page 68 lists the available bottle filling parameters.

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4 Using the Pump
Setting up the Agilent 1260 Infinity Binary Pump G1312B with the Instrument Control Interface

Table 6 Bottle Filling Parameters

Parameter Limits Description

• Total 0 – 1000 L This is the capacity (maximum possible volume) in liter of the solvent bottle. In
Volume default: 0 L combination with the actual volume, this parameter is used for calculating and
displaying the relative liquid level.

• Actual 0 – 1000 L After filling the solvent bottles, enter the actual volumes into these boxes. The
Volume default: 0 L Actual Volume must not be larger than the Total Volume of the bottle.

• Prevent default: unchecked If this option is checked, the pump won’t start a new run if the solvent level in
analysis....... one or more bottles is below the minimum volume. Enter a minimum volume in
liter, which considers the position of the solvent inlet and size/shape of the
solvent bottle such that no air is drawn if the actual volume gets close to this
limit.

• Turn pump default: unchecked If this option is checked, the pump will turn off before air is aspirated. However,
off... the residual solvent volume has been calculated for 1 L solvent bottles and may
be too small for large bottles or other vessels.

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 Using the Pump 4
Solvent Information

Solvent Information

Observe the following recommendations on the use of solvents.

• Follow recommendations for avoiding the growth of algae, see “Algae
Growth in HPLC Systems” on page 74
• Small particles can permanently block capillaries and valves. Therefore,
always filter solvents through 0.4 µm filters.
• Avoid or minimize the use of solvents that may corrode parts in the
flow path. Consider specifications for the pH range given for different
materials like flow cells, valve materials etc. and recommendations in
subsequent sections.

Materials in Flow Path

Following materials are used in the flow path of this module:

Table 7 Materials in flow path

Part Materials

SSV PEEK, FFKM

Active inlet valve SST, sapphire, ruby, ceramic, PTFE
Outlet valve SST, gold, ruby, ZrO2-based ceramic, tantalum
Adapter SST, gold
Pump head (body) SST
Pistons Sapphire
Piston seals/wash seals PTFE, SST (reversed phase) or UHMW-PE, SST (normal phase)
Pressure sensor SST
Purge valve SST, gold, PTFE, ceramic
Damping unit SST, gold
Capillaries/fittings SST
Tubings PTFE

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4 Using the Pump
Solvent Information

Material Information
Materials in the flow path are carefully selected based on Agilent’s
experiences in developing highest quality instruments for HPLC analysis
over several decades. These materials exhibit excellent robustness under
typical HPLC conditions. For any special conditions, please consult the
material information section or contact Agilent.

Disclaimer
Subsequent data were collected from external resources and are meant as
a reference. Agilent cannot guarantee the correctness and completeness of
such information. Data is based on compatibility libraries, which are not
specific for estimating the long- term life time under specific but highly
variable conditions of UHPLC systems, solvents, solvent mixtures and
samples. Information can also not be generalized due to catalytic effects of
impurities like metal ions, complexing agents, oxygen etc. Apart from pure
chemical corrosion, other effects like electro corrosion, electrostatic
charging (especially for non- conductive organic solvents), swelling of
polymer parts etc. need to be considered. Most data available refers to
room temperature (typically 20 – 25 °C, 68 – 77 °F). If corrosion is
possible, it usually accelerates at higher temperatures. If in doubt, please
consult technical literature on chemical compatibility of materials.

PEEK
PEEK (Polyether- Ether Ketones) combines excellent properties regarding
biocompatibility, chemical resistance, mechanical and thermal stability.
PEEK is therefore the material of choice for UHPLC and biochemical
instrumentation.
It is stable in a wide pH range, and inert to many common solvents.
There is still a number of known incompatibilities with chemicals such as
chloroform, methylene chloride, THF, DMSO, strong acids (nitric acid >
10 %, sulphuric acid > 10 %, sulfonic acids, trichloroacetic acid), halogenes
or aequous halogene solutions, phenol and derivatives (cresols, salicylic
acid etc.).

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 Using the Pump 4
Solvent Information

Polyimide
Agilent uses semi- crystalline polyimide for rotor seals in valves and needle
seats in autosamplers. One supplier of polyimide is DuPont, which brands
polyimide as Vespel, which is also used by Agilent.
Polyimide is stable in a pH range between 1 and 10 and in most organic
solvents. It is incompatible with concentrated mineral acids (e.g. sulphuric
acid), glacial acetic acid, DMSO and THF. It is also degraded by
nucleophilic substances like ammonia (e.g. ammonium salts in basic
conditions) or acetates.

Polyethylene (PE)
Agilent uses UHMW (ultra- high molecular weight)- PE/PTFE blends for
yellow piston and wash seals, which are used in 1290 Infinity pumps and
for normal phase applications in 1260 Infinity pumps.
Polyethylene has a good stability for most common inorganic solvents
including acids and bases in a pH range of 1 to 12.5 . It is compatible to
many organic solvents used in chromatographic systems like methanol,
acetonitrile and isopropanol. It has limited stability with aliphatic,
aromatic and halogenated hydrocarbons, THF, phenol and derivatives,
concentrated acids and bases. For normal phase applications, the
maximum pressure should be limited to 200 bar.

Tantalum (Ta)
Tantalum is inert to most common HPLC solvents and almost all acids
except fluoric acid and acids with free sulfur trioxide. It can be corroded
by strong bases (e.g. hydroxide solutions > 10 %, diethylamine). It is not
recommended for the use with fluoric acid and fluorides.

Stainless Steel (ST)

Stainless steel is inert against many common solvents. It is stable in the
presence of acids and bases in a pH range of 1 to 12.5 . It can be
corroded by acids below pH 2.3 . It can also corrode in following solvents:
• Solutions of alkali halides, their respective acids (for example, lithium
iodide, potassium chloride, and so on) and aqueous solutions of
halogens.

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4 Using the Pump
Solvent Information

• High concentrations of inorganic acids like nitric acid, sulfuric acid and
organic solvents especially at higher temperatures (replace, if your
chromatography method allows, by phosphoric acid or phosphate buffer
which are less corrosive against stainless steel).
• Halogenated solvents or mixtures which form radicals and/or acids, for
example:
2 CHCl3 + O2→ 2 COCl2 + 2 HCl
This reaction, in which stainless steel probably acts as a catalyst,
occurs quickly with dried chloroform if the drying process removes the
stabilizing alcohol.
• Chromatographic grade ethers, which can contain peroxides (for
example, THF, dioxane, di- isopropylether). Such ethers should be
filtered through dry aluminium oxide which adsorbs the peroxides.
• Solutions of organic acids (acetic acid, formic acid, and so on) in
organic solvents. For example, a 1 % solution of acetic acid in methanol
will attack steel.
• Solutions containing strong complexing agents (for example, EDTA,
ethylene diamine tetra- acetic acid).
• Mixtures of carbon tetrachloride with 2- propanol or THF.

Diamond-Like Carbon (DLC)

Diamond- Like Carbon is inert to almost all common acids, bases and
solvents. There are no documented incompatibilities for HPLC applications.

Fused silica and Quartz (SiO2)

Fused silica is used in 1290 Infinity Flow Cells and capillaries. Quartz is
used for classical flow cell windows. It is inert against all common
solvents and acids except hydrofluoric acid and acidic solvents containing
fluorides. It is corroded by strong bases and should not be used above pH
12 at room temperature. The corrosion of flow cell windows can negatively
affect measurement results. For a pH greater than 12, the use of flow cells
with sapphire windows is recommended.

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 Using the Pump 4
Solvent Information

Gold
Gold is inert to all common HPLC solvents, acids and bases within the
specified pH range. It can be corroded by complexing cyanides and
concentrated acids like aqua regia.

Zirconium Oxide (ZrO2)

Zirconium Oxide is inert to almost all common acids, bases and solvents.
There are no documented incompatibilities for HPLC applications.

Platinum/Iridium
Platinum/Iridium is inert to almost all common acids, bases and solvents.
There are no documented incompatibilities for HPLC applications.

Fluorinated polymers (PTFE, PFA, FEP, FFKM)

Fluorinated polymers like PTFE (polytetrafluorethylene), PFA
(perfluoroalkoxy) and FEP (fluorinated ethylene propylene) are inert to
almost all common acids, bases, and solvents. FFKM is perfluorinated
rubber, which is also resistant to most chemicals. As an elastomer, it may
swell in some organic solvents like halogenated hydrocarbons.
TFE/PDD copolymer tubings, which are used in all Agilent degassers
except G1322A, are not compatible with fluorinated solvents like Freon,
Fluorinert, or Vertrel. They have limited life time in the presence of
Hexafluoroisopropanol (HFIP). To ensure the longest possible life with
HFIP, it is best to dedicate a particular chamber to this solvent, not to
switch solvents, and not to let dry out the chamber. For optimizing the life
of the pressure sensor, do not leave HFIP in the chamber when the unit is
off.

Sapphire, Ruby and Al2O3-based ceramics

Sapphire, ruby and ceramics based on aluminum oxide Al2O3 are inert to
almost all common acids, bases and solvents. There are no documented
incompatibilities for HPLC applications.

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4 Using the Pump
Algae Growth in HPLC Systems

Algae Growth in HPLC Systems

The presence of algae in HPLC systems can cause a variety of problems

that may be incorrectly diagnosed as instrument or application problems.
Algae grow in aqueous media, preferably in a pH range of 4- 8. Their
growth is accelerated by buffers, for example phosphate or acetate. Since
algae grow through photosynthesis, light will also stimulate their growth.
Even in distilled water small- sized algae grow after some time.

Instrumental Problems Associated With Algae

Algae deposit and grow everywhere within the HPLC system causing:
• Blocked solvent filters or deposits on inlet or outlet valves resulting in
unstable flow, composition or gradient problems or a complete failure of
the pump.
• Small pore high pressure solvent filters, usually placed before the
injector to plug resulting in high system pressure.
• PTFE frits blockage leading to increased system pressure.
• Column filters to plug giving high system pressure.
• Flow cell windows of detectors to become dirty resulting in higher noise
levels (since the detector is the last module in the flow path, this
problem is less common).

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 Using the Pump 4
Algae Growth in HPLC Systems

How to Prevent and/or Reduce the Algae Problem

• Always use freshly prepared solvents, especially use demineralized
water which was filtered through about 0.2 µm filters.
• Never leave mobile phase in the instrument for several days without
flow.
• Always discard old mobile phase.
• Use the amber solvent bottle (Solvent bottle, amber (9301- 1450))
supplied with the instrument for your aqueous mobile phase.
• If possible add a few mg/l sodium azide or a few percent organic
solvent to the aqueous mobile phase.

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4 Using the Pump
Prevent Blocking of Solvent Filters

Prevent Blocking of Solvent Filters

Contaminated solvents or algae growth in the solvent bottle will reduce

the lifetime of the solvent filter and will influence the performance of the
module. This is especially true for aqueous solvents or phosphate buffers
(pH 4 to 7). The following suggestions will prolong lifetime of the solvent
filter and will maintain the performance of the module.
• Use a sterile, if possible amber, solvent bottle to slow down algae
growth.
• Filter solvents through filters or membranes that remove algae.
• Exchange solvents every two days or refilter.
• If the application permits add 0.0001 – 0.001 M sodium azide to the
solvent.
• Place a layer of argon on top of your solvent.
• Avoid exposure of the solvent bottle to direct sunlight.

Never use the system without solvent filter installed.

NOTE

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 Using the Pump 4
Prevent Blocking of Solvent Filters

Checking the Solvent Filters

The solvent filters are located on the low- pressure side of the binary
pump. A blocked filter therefore does not necessarily affect the high
pressure readings of the pump. The pressure readings cannot be used to
check whether the filters are blocked or not. If the solvent cabinet is
placed on top of the binary pump, the filter condition can be checked in
the following way:
Remove the solvent inlet tube from the inlet port of the solvent selection
valve or the adapter at the active inlet valve. If the filter is in good
condition, the solvent will freely drip out of the solvent tube (due to
hydrostatic pressure). If the solvent filter is partly blocked only very little
solvent will drip out of the solvent tube.

When opening capillary or tube fittings, solvents may leak out.

WA R N I N G
The handling of toxic and hazardous solvents and reagents can carry health risks.
➔ Observe appropriate safety procedures (for example, wear goggles, safety gloves
and protective clothing) as described in the material handling and safety data sheet
supplied by the solvent vendor, especially when toxic or hazardous solvents are
used.

Cleaning the Solvent Filters

• Remove the blocked solvent filter from the bottle- head assembly and
place it in a beaker with concentrated nitric acid (35%) for one hour.
• Thoroughly flush the filter with HPLC- grade water (remove all nitric
acid, some capillary columns can be damaged by nitric acid).
• Replace the filter.

Never use the system without solvent filter installed.

NOTE

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4 Using the Pump
Prevent Blocking of Solvent Filters

78 1260 Infinity Binary Pump User Manual

 1260 Infinity Binary Pump User Manual

5
Optimizing Performance
When to Use a Vacuum Degasser 80
Operational Hints for the Vacuum Degasser 80
When to Use the Active Seal Wash Option 81
When to Use Alternative Seals 82
When to Use the Low Volume Mixer 83
When to Remove Damper and Mixer 84
Convert the Binary Pump to Low Delay Volume Mode 85
How to Optimize the Compressibility Compensation Setting 87
Solvent Compressibility Calibration 87
Optimization of Legacy Compressibility Settings 88

This chapter gives information on how to optimize the performance of the

Binary Pump under special operational conditions.

Agilent Technologies 79
5 Optimizing Performance
When to Use a Vacuum Degasser

When to Use a Vacuum Degasser

A degasser removes air, which is dissolved in any solvent. When solvents

are heated or mixed with other solvents, air can leave the solvent and
form small bubbles. Over time, these bubbles accumulate and can cause
pressure fluctuations which may finally result in retention time shifts.
The G4225A High- performance Degasser and the G1322A Vacuum Degasser
are external degassers that are designed to be used with Agilent 1260
Infinity binary pumps or 1100/1200 Series pumps. 1260 Infinity
quaternary pumps, 1290 Infinity pumps, and 1120/1220 Infinity pumps
have built- in degassers.
A degasser is needed for low pressure mixing pumps like Agilent
quaternary pumps. High pressure mixing pumps like Agilent binary pumps
are more robust with respect to bubble formation, but a degasser is
recommended for best performance. Additionally, a degasser is highly
recommended for the following applications:
• Your detector is used with maximum sensitivity in the low UV
wavelength range.
• Your application requires highest injection precision.
• Your application requires highest retention- time reproducibility (flow
rates below 0.5 mL/min).
• The binary pump is used with bypassed damper and mixer.

Operational Hints for the Vacuum Degasser

If you are using the vacuum degasser for the first time, if the vacuum
degasser was switched off for any length of time (for example, overnight),
or if the vacuum degasser chambers are empty, you have to prime the
vacuum degasser before running an analysis. Priming is usually done by
pumping at a high flow rate (3 – 5 mL/min). Alternatively, a syringe can
be used to draw the solvent through the (empty) degasser if the pump
does not aspirate the solvent by itself. For details see “Initial Priming” on
page 54.
For more information see the Agilent 1260 Infinity Standard Degasser
User Manual.

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 Optimizing Performance 5
When to Use the Active Seal Wash Option

When to Use the Active Seal Wash Option

Concentrated buffer solutions will reduce the lifetime of the seals and
pistons in your binary pump. The active seal wash option allows to
maintain the seal lifetime by flushing the low pressure side of the seals
with a wash solvent.
The seal wash option is strongly recommended if buffer concentrations of
0.1 M or higher are used regularly with the pump.
The active seal wash option kit can be ordered by quoting Active Seal
Wash Upgrade Product including Service (G1399A).
The seal wash option comprises a peristaltic pump, secondary seals,
gaskets, seal holders and tubing for both pump heads. A bottle of
premixed water/isopropanol (90 /10 vol%) is placed in the solvent cabinet
and connected to the peristaltic pump as described in the technical note
that comes with the active seal wash kit.
Always use a mixture of HPLC- grade water (90 %) and isopropanol (10 %)
as wash solvent. This mixture prevents bacteria growth in the wash bottle
and reduces the surface tension of the water.

In order to avoid accumulation of buffer salts or impurities, regularly replace the washing
NOTE solution using fresh solvents.

The operation of the peristaltic pump can be controlled from the data
system or the Instant Pilot.
For adding a seal- wash option, please contact your local Agilent
Technologies service representative.

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5 Optimizing Performance
When to Use Alternative Seals

When to Use Alternative Seals

The standard seals for the binary pump can be used for most applications.
However, normal phase applications (for example, hexane) are not
compatible with the standard seals. They cause extremely high abrasion
and significantly shorten seal life time.
For the use with normal phase applications special polyethylene pistons
seals (yellow color, PE seals (pack of 2) (0905- 1420)) are available. These
seals have less abrasion compared to the standard seals.

The seal wear-in procedure causes problems to the normal phase seals (yellow).
WA R N I N G
They will be destroyed by the procedure.
➔ DO NOT apply the seal wear-in procedure performed to normal phase seals.

1 Remove the standard seals from the pump head (“Maintenance of a

Pump Head without Seal Wash” on page 159).
2 Install normal phase seals.

Polyethylene seals have a limited pressure range of 0–200 bar. When used above 200 bar,
NOTE their lifetime will be significantly reduced.

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 Optimizing Performance 5
When to Use the Low Volume Mixer

When to Use the Low Volume Mixer

The Low volume mixer ( 200 µL) (5067- 1565) is designed for use with the
1260 Infinity Binary LC System in low delay volume mode. This
configuration is typically used for 2.1 mm i.d., 1.8 µm particle size
columns, where emphasis is put on S/N ratio. The low volume mixer helps
mixing gradients starting with a low concentration of organic solvents,
which can cause noise on the baseline. The maximum benefit of the mixer
is achieved using the mixer together with FW revisions A.06.06 or higher.
The low volume mixer is installed with the corresponding holder (Low
Volume Mixer Holder (G1312- 00003)).

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5 Optimizing Performance
When to Remove Damper and Mixer

When to Remove Damper and Mixer

The binary pump is equipped with a pressure pulsation damper and a

static mixer. The total delay volume of the pump is 600 – 800 µL
(depending on system pressure). The mixer has a volume of 400 µL.
For applications that require lowest delay volume (e.g. fast gradient
methods or gradient applications with low flow rates), damper and mixer
can be bypassed.

HiVcYVgYXdc[^\jgVi^dc AdlYZaVnkdajbZXdc[^\jgVi^dc

Figure 20 Flow Path Modifications of the Binary Pump

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 Optimizing Performance 5
When to Remove Damper and Mixer

Convert the Binary Pump to Low Delay Volume Mode

The binary pump is delivered in standard configuration (damper and
mixer connected). This paragraph shows how to bypass damper and mixer
and convert the pump to low delay volume mode.
Configurations where only damper or mixer are disconnected while the
other part is still in line are not supported by Agilent Technologies.

Tools required p/n Description

8710-0510 Wrench, 1/4 - 5/16 inch, open
Wrench, 14 mm
Hex driver open, 1/4 inch

Preparations Flush the system (water if buffers were used, otherwise isopropanol).
Turn the flow off.

1 Remove the front cover by pressing the clip fastener on 2 Use the 1/4 inch hex driver to remove fitting B from port
both sides of the cover. 2 of the pressure sensor.

'

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5 Optimizing Performance
When to Remove Damper and Mixer

3 Fold capillary end B away. It remains 4 Connect fitting A to port 2 of the pressure sensor. Seal
unconnected.Disconnect fitting A from outlet 1 of the port 1 of the mixer with a plastic blank nut.
mixer.

6 6
7 7
& &
' '

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 Optimizing Performance 5
How to Optimize the Compressibility Compensation Setting

How to Optimize the Compressibility Compensation Setting

When a solvent is metered at ambient pressure and compressed to a

higher pressure, the volume decreases depending on its compressibility.
Solvent compressibility is a non- linear function of pressure and
temperature. It is specific for each solvent.
In order to deliver the desired flow accurately at all pressures, Agilent
pumps use a compressibility compensation. For standard LC applications,
e.g. using a 400 bar binary pump, an average compressibility value for the
solvent is sufficient.
For the 600 bar 1260 Infinity Binary Pump , the pressure- dependency of a
solvent compressibility needs to be considered. It is determined at
different pressures between 0 – 600 bar. The pump uses the obtained
non- linear function to select the correct compressibility value for the
actual pump pressure. Compressibility data for the most common solvents
is readily available in the pump firmware.
The compensation algorithm is so powerful that the damper and mixer can
be removed from the pump flow path at low flow rate while the pressure
ripple and composition ripple remain at low levels.
For method compatibility reasons, the legacy compressibility compensation
is still available.

Solvent Compressibility Calibration

Unlisted or premixed solvents can be calibrated with the Solvent
Compressibility Calibration function. For a detailed description, see
“Binary Pump Solvent Compressibility Calibration” on page 133.

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5 Optimizing Performance
How to Optimize the Compressibility Compensation Setting

Optimization of Legacy Compressibility Settings

The compressibility compensation default settings are 50 × 10- 6 /bar (best
for most aqueous solutions) for pump head A and 115 × 10- 6 /bar (to suit
organic solvents) for pump head B. The settings represent average values
for aqueous solvents (A side) and organic solvents (B side). Therefore it is
always recommended to use the aqueous solvent on the A side of the
pump and the organic solvent on the B side. Under normal conditions, the
default settings reduce the pressure pulsation to below 2 % of system
pressure, which is sufficient for most applications. If the compressibility
values for the solvents used differ from the default settings, it is
recommended to change the compressibility values accordingly.
Compressibility settings can be optimized by using the values for various
solvents described in Table 8 on page 89. If the solvent in use is not listed
in the compressibility table, when using premixed solvents and if the
default settings are not sufficient for your application, the following
procedure can be used to optimize the compressibility settings:
1 Start channel A of the binary pump with the required flow rate.
2 Before starting the optimization procedure, the flow must be stable. Use
degassed solvent only. Check the tightness of the system with the
pressure test (see “(System) Pressure Test” on page 126).
3 Your pump must be connected to an Agilent data system or Instant
Pilot, the pressure- and %- ripple can be monitored with one of these
instruments, otherwise connect a signal cable between the pressure
output of the isocratic pump and a recording device (for example, 339X
integrator) and set following parameters.
Zero 50 % Att 2^3 Chart Speed 10 cm/min
4 Start the recording device in plot mode.
5 Starting with a compressibility setting of 40 × 10- 6 /bar, increase the
value in steps of 10. Re- zero the integrator as required. The
compressibility compensation setting that generates the smallest
pressure ripple is the optimum value for your solvent composition.
6 Repeat step 1 through step 5 for the B channel of your binary pump.

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 Optimizing Performance 5
How to Optimize the Compressibility Compensation Setting

Table 8 Solvent Compressibility

Solvent (pure) Compressibility (10-6/bar)

Acetone 126

Acetonitrile 115

Benzene 95

Carbon tetrachloride 110

Chloroform 100

Cyclohexane 118

Ethanol 114

Ethyl acetate 104

Heptane 120

Hexane 150

Isobutanol 100

Isopropanol 100

Methanol 120

1-Propanol 100

Toluene 87

Water 46

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5 Optimizing Performance
How to Optimize the Compressibility Compensation Setting

90 1260 Infinity Binary Pump User Manual

 1260 Infinity Binary Pump User Manual

6
Troubleshooting and Diagnostics
Overview of the Module’s Indicators and Test Functions 92
Status Indicators 94
Power Supply Indicator 94
Module Status Indicator 95
User Interfaces 96
Agilent Lab Advisor Software 97

Overview of the troubleshooting and diagnostic features.

Agilent Technologies 91
6 Troubleshooting and Diagnostics
Overview of the Module’s Indicators and Test Functions

Overview of the Module’s Indicators and Test Functions

Status Indicators
The module is provided with two status indicators which indicate the
operational state (prerun, run, and error states) of the module. The status
indicators provide a quick visual check of the operation of the module.

Error Messages
In the event of an electronic, mechanical or hydraulic failure, the module
generates an error message in the user interface. For each message, a
short description of the failure, a list of probable causes of the problem,
and a list of suggested actions to fix the problem are provided (see
chapter Error Information).

Test Functions
A series of test functions are available for troubleshooting and operational
verification after exchanging internal components (see Tests and
Calibrations).

Pressure Test
The Pressure Test is a quick test designed to determine the pressure
tightness of the system (i.e. the high pressure flow path between pump
and column). After exchanging flow path components (e.g. pump seals or
injection seal), use this test to verify the system is pressure tight, see
“(System) Pressure Test” on page 126.

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 Troubleshooting and Diagnostics 6
Overview of the Module’s Indicators and Test Functions

Solvent Compressibility Calibration

Solvent compressibility is a function of solvent type and pressure. In order
to optimize flow accuracy and pressure ripple, the compressibility of the
solvent must be considered. The binary pump firmware contains
compressibility parameters for most commonly used solvents. A
compressibility calibration function is available to generate compressibility
data for unlisted solvents (see “Binary Pump Solvent Compressibility
Calibration” on page 133). The compressibility data are stored in an XML
file and can be transferred to other G1312B pumps.

Pump Elasticity Calibration

Various parts in the flow path of the binary pump have a certain elasticity
which needs to be compensated to obtain the lowest pressure- , flow- and
composition ripple possible. This is done by running an elasticity
calibration after maintenance and major repairs. For details see “Pump
Elasticity Calibration” on page 135.

Leak Rate Test

The Leak Rate Test is a diagnostic test designed to determine the pressure
tightness of the pump components. When a problem with the pump is
suspected, use this test to help troubleshoot the pump and its pumping
performance, see “Leak Rate Test” on page 137.

Diagnostic Signals
The pump has several signals (pressure, voltages and piston movement)
that can be used for diagnosing pressure stability, composition and flow
problems (see chapter Diagnostic Signals).

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6 Troubleshooting and Diagnostics
Status Indicators

Status Indicators

Two status indicators are located on the front of the module. The lower
left indicates the power supply status, the upper right indicates the
module status.

HiVijh>cY^XVidg

EdlZghl^iX]

HZg^VacjbWZg

Figure 21 Location of Status Indicators

Power Supply Indicator

The power supply indicator is integrated into the main power switch.
When the indicator is illuminated (green) the power is ON.

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 Troubleshooting and Diagnostics 6
Status Indicators

Module Status Indicator

The module status indicator indicates one of six possible module
conditions:
• When the status indicator is OFF (and power switch light is on), the
module is in a prerun condition, and is ready to begin an analysis.
• A green status indicator, indicates the module is performing an analysis
(run mode).
• A yellow indicator indicates a not- ready condition. The module is in a
not- ready state when it is waiting for a specific condition to be reached
or completed (for example, immediately after changing a set point), or
while a self- test procedure is running.
• An error condition is indicated when the status indicator is red. An
error condition indicates the module has detected an internal problem
which affects correct operation of the module. Usually, an error
condition requires attention (e.g. leak, defective internal components).
An error condition always interrupts the analysis.
If the error occurs during analysis, it is propagated within the LC
system, i.e. a red LED may indicate a problem of a different module.
Use the status display of your user interface for finding the root
cause/module of the error.
• A blinking indicator indicates that the module is in resident mode (e.g.
during update of main firmware).
• A fast blinking indicator indicates that the module is in a low- level
error mode. In such a case try to re- boot the module or try a cold- start
(see “Special Settings” on page 231. Then try a firmware update (see
“Replacing Module Firmware” on page 181). If this does not help, a
main board replacement is required.

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6 Troubleshooting and Diagnostics
User Interfaces

User Interfaces

Depending on the user interface, the available tests vary. Some

descriptions are only available in the Service Manual.

Test Instant Pilot G4208A Agilent Lab Advisor

Pressure Test Yes Yes

Valve Test No Yes

Solvent compressibility No Yes

calibration

Pump elasticity calibration No Yes

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 Troubleshooting and Diagnostics 6
Agilent Lab Advisor Software

Agilent Lab Advisor Software

The Agilent Lab Advisor software is a standalone product that can be

used with or without data system. Agilent Lab Advisor software helps to
manage the lab for high quality chromatographic results and can monitor
in real time a single Agilent LC or all the Agilent GCs and LCs configured
on the lab intranet.
Agilent Lab Advisor software provides diagnostic capabilities for all
Agilent 1200 Infinity Series modules. This includes diagnostic capabilities,
calibration procedures and maintenance routines for all the maintenance
routines.
The Agilent Lab Advisor software also allows users to monitor the status
of their LC instruments. The Early Maintenance Feedback (EMF) feature
helps to carry out preventive maintenance. In addition, users can generate
a status report for each individual LC instrument. The tests and diagnostic
features as provided by the Agilent Lab Advisor software may differ from
the descriptions in this manual. For details refer to the Agilent Lab
Advisor software help files.
The Instrument Utilities is a basic version of the Lab Advisor with limited
functionality required for installation, use and maintenance. No advanced
repair, troubleshooting and monitoring functionality is included.

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6 Troubleshooting and Diagnostics
Agilent Lab Advisor Software

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1260 Infinity Binary Pump User Manual

7
Error Information
What Are Error Messages 101
General Error Messages 102
Timeout 102
Shutdown 103
Remote Timeout 104
Lost CAN Partner 104
Leak 105
Leak Sensor Open 105
Leak Sensor Short 106
Compensation Sensor Open 106
Compensation Sensor Short 107
Fan Failed 107
Open Cover 108
Module Error Messages 109
Solvent Zero Counter 109
Pressure Above Upper Limit 109
Pressure Below Lower Limit 110
Pressure Signal Missing 110
Valve Failed 111
Missing Pressure Reading 112
Wrong Pump Configuration 112
Electronic Fuse of SSV Open 113
AIV Fuse 113
Temperature Out of Range 114
Temperature Limit Exceeded 115
Motor-Drive Power 116
Encoder Missing 117
Inlet-Valve Missing 117

Agilent Technologies 99
7 Error Information
Agilent Lab Advisor Software

Servo Restart Failed 118

Pump Head Missing 119
Index Limit 120
Index Adjustment 121
Index Missing 122
Stroke Length 122
Initialization Failed 123

This chapter describes the meaning of error messages, and provides

information on probable causes and suggested actions how to recover from
error conditions.

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 Error Information 7
What Are Error Messages

What Are Error Messages

Error messages are displayed in the user interface when an electronic,

mechanical, or hydraulic (flow path) failure occurs which requires
attention before the analysis can be continued (for example, repair, or
exchange of consumables is necessary). In the event of such a failure, the
red status indicator at the front of the module is switched on, and an
entry is written into the module logbook.
If an error occurs outside a method run, other modules will not be
informed about this error. If it occurs within a method run, all connected
modules will get a notification, all LEDs get red and the run will be
stopped. Depending on the module type, this stop is implemented
differently. For example, for a pump the flow will be stopped for safety
reasons. For a detector, the lamp will stay on in order to avoid
equilibration time. Depending on the error type, the next run can only be
started, if the error has been resolved, for example liquid from a leak has
been dried. Errors for presumably single time events can be recovered by
switching on the system in the user interface.
Special handling is done in case of a leak. As a leak is a potential safety
issue and may have occurred at a different module from where it has been
observed, a leak always causes a shutdown of all modules, even outside a
method run.
In all cases, error propagation is done via the CAN bus or via an APG
remote cable (see documentation for the APG interface).

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7 Error Information
General Error Messages

General Error Messages

Timeout
Error ID: 0062
The timeout threshold was exceeded.

Probable cause Suggested actions

1 The analysis was completed successfully, Check the logbook for the occurrence and
and the timeout function switched off the source of a not-ready condition. Restart the
module as requested. analysis where required.

2 A not-ready condition was present during a Check the logbook for the occurrence and
sequence or multiple-injection run for a source of a not-ready condition. Restart the
period longer than the timeout threshold. analysis where required.

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 Error Information 7
General Error Messages

Shutdown
Error ID: 0063
An external instrument has generated a shutdown signal on the remote
line.
The module continually monitors the remote input connectors for status
signals. A LOW signal input on pin 4 of the remote connector generates
the error message.

Probable cause Suggested actions

1 Leak detected in another module with a Fix the leak in the external instrument before
CAN connection to the system. restarting the module.

2 Leak detected in an external instrument Fix the leak in the external instrument before
with a remote connection to the system. restarting the module.

3 Shut-down in an external instrument with a Check external instruments for a shut-down

remote connection to the system. condition.

4 The degasser failed to generate sufficient Check the vacuum degasser for an error
vacuum for solvent degassing. condition. Refer to the Service Manual for the
degasser or the 1260 pump that has the
degasser built-in.

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7 Error Information
General Error Messages

Remote Timeout
Error ID: 0070
A not- ready condition is still present on the remote input. When an
analysis is started, the system expects all not- ready conditions (for
example, a not- ready condition during detector balance) to switch to run
conditions within one minute of starting the analysis. If a not- ready
condition is still present on the remote line after one minute the error
message is generated.

Probable cause Suggested actions

1 Not-ready condition in one of the Ensure the instrument showing the not-ready
instruments connected to the remote line. condition is installed correctly, and is set up
correctly for analysis.
2 Defective remote cable. Exchange the remote cable.

3 Defective components in the instrument Check the instrument for defects (refer to the
showing the not-ready condition. instrument’s documentation).

Lost CAN Partner

Error ID: 0071
During an analysis, the internal synchronization or communication
between one or more of the modules in the system has failed.
The system processors continually monitor the system configuration. If one
or more of the modules is no longer recognized as being connected to the
system, the error message is generated.

Probable cause Suggested actions

1 CAN cable disconnected. • Ensure all the CAN cables are connected
correctly.
• Ensure all CAN cables are installed
correctly.
2 Defective CAN cable. Exchange the CAN cable.

3 Defective main board in another module. Switch off the system. Restart the system, and
determine which module or modules are not
recognized by the system.

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 Error Information 7
General Error Messages

Leak
Error ID: 0064
A leak was detected in the module.
The signals from the two temperature sensors (leak sensor and
board- mounted temperature- compensation sensor) are used by the leak
algorithm to determine whether a leak is present. When a leak occurs, the
leak sensor is cooled by the solvent. This changes the resistance of the
leak sensor which is sensed by the leak- sensor circuit on the main board.

Probable cause Suggested actions

1 Loose fittings. Ensure all fittings are tight.

2 Broken capillary. Exchange defective capillaries.

3 Loose or leaking purge valve, active inlet Ensure pump components are seated correctly.
valve, or outlet valve. If there are still signs of a leak, exchange the
appropriate seal (purge valve, active inlet valve,
outlet valve).
4 Defective pump seals. Exchange the pump seals.

Leak Sensor Open

Error ID: 0083
The leak sensor in the module has failed (open circuit).
The current through the leak sensor is dependent on temperature. A leak
is detected when solvent cools the leak sensor, causing the leak- sensor
current to change within defined limits. If the current falls outside the
lower limit, the error message is generated.

Probable cause Suggested actions

1 Leak sensor not connected to the main Please contact your Agilent service
board. representative.

2 Defective leak sensor. Please contact your Agilent service

representative.
3 Leak sensor incorrectly routed, being Please contact your Agilent service
pinched by a metal component. representative.

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7 Error Information
General Error Messages

Leak Sensor Short

Error ID: 0082
The leak sensor in the module has failed (short circuit).
The current through the leak sensor is dependent on temperature. A leak
is detected when solvent cools the leak sensor, causing the leak sensor
current to change within defined limits. If the current increases above the
upper limit, the error message is generated.

Probable cause Suggested actions

1 Defective leak sensor. Please contact your Agilent service

representative.

2 Leak sensor incorrectly routed, being Please contact your Agilent service
pinched by a metal component. representative.

Compensation Sensor Open

Error ID: 0081
The ambient- compensation sensor (NTC) on the main board in the module
has failed (open circuit).
The resistance across the temperature compensation sensor (NTC) on the
main board is dependent on ambient temperature. The change in
resistance is used by the leak circuit to compensate for ambient
temperature changes. If the resistance across the sensor increases above
the upper limit, the error message is generated.

Probable cause Suggested actions

1 Defective main board. Please contact your Agilent service

representative.

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 Error Information 7
General Error Messages

Compensation Sensor Short

Error ID: 0080
The ambient- compensation sensor (NTC) on the main board in the module
has failed (open circuit).
The resistance across the temperature compensation sensor (NTC) on the
main board is dependent on ambient temperature. The change in
resistance is used by the leak circuit to compensate for ambient
temperature changes. If the resistance across the sensor falls below the
lower limit, the error message is generated.

Probable cause Suggested actions

1 Defective main board. Please contact your Agilent service
representative.

Fan Failed
Error ID: 0068
The cooling fan in the module has failed.
The hall sensor on the fan shaft is used by the main board to monitor the
fan speed. If the fan speed falls below a certain limit for a certain length
of time, the error message is generated.
This limit is given by 2 revolutions/second for longer than 5 seconds.
Depending on the module, assemblies (e.g. the lamp in the detector) are
turned off to assure that the module does not overheat inside.

Probable cause Suggested actions

1 Fan cable disconnected. Please contact your Agilent service
representative.
2 Defective fan. Please contact your Agilent service
representative.
3 Defective main board. Please contact your Agilent service
representative.
4 Improperly positioned cables or wires Please contact your Agilent service
obstructing fan blades. representative.

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7 Error Information
General Error Messages

Open Cover
Error ID: 0205
The top foam has been removed.
The sensor on the main board detects when the top foam is in place. If
the foam is removed, the fan is switched off, and the error message is
generated.

Probable cause Suggested actions

1 The top foam was removed during Please contact your Agilent service
operation. representative.

2 Foam not activating the sensor. Please contact your Agilent service
representative.

3 Defective sensor or main board. Please contact your Agilent service

representative.

4 Rear of the module is exposed to strong Ensure that the rear of module is not directly
direct sunlight. exposed to strong sunlight.

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 Error Information 7
Module Error Messages

Module Error Messages

Solvent Zero Counter

Error ID: 2055
The error message is triggered if the remaining volume in a solvent bottle
falls below the set limit.

Probable cause Suggested actions

1 Volume in bottle below specified volume. Refill bottles and reset solvent counters.

2 Incorrect setting. Make sure the set solvent volume matches the
actual bottle filling and set the shutoff limit to a
reasonable value (e.g. 100 mL for 1 L bottles)

Pressure Above Upper Limit

Error ID: 2014, 2500
The system pressure has exceeded the upper pressure limit.

Probable cause Suggested actions

1 Upper pressure limit set too low. Ensure the upper pressure limit is set to a value
suitable for the analysis.

2 Blockage in the flowpath (after the damper). Check for blockage in the flowpath. The
following components are particularly subject
to blockage: inline filter frit, needle
(autosampler), seat capillary (autosampler),
sample loop (autosampler), column frits and
capillaries with small internal diameters (e.g.
50 µm ID).

3 Defective damper. Please contact your Agilent service

representative.

4 Defective main board. Please contact your Agilent service

representative.

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7 Error Information
Module Error Messages

Pressure Below Lower Limit

Error ID: 2015, 2501
The system pressure has fallen below the lower pressure limit.

Probable cause Suggested actions

1 Lower pressure limit set too high. Ensure the lower pressure limit is set to a value
suitable for the analysis.
2 Air bubbles in the mobile phase. • Make sure that the degasser is in flow path
and works correctly. Purge the module.
• Ensure solvent inlet filters are not blocked.
3 Leak. • Inspect the pump head, capillaries and
fittings for signs of a leak.
• Purge the module. Run a pressure test to
determine whether the seals or other
module components are defective.
4 Defective damper. Please contact your Agilent service
representative.
5 Defective main board. Please contact your Agilent service
representative.

Pressure Signal Missing

Error ID: 2016
The pressure signal of the damper is missing.
The pressure signal of the damper must be within a specific voltage range.
If the pressure signal is missing, the processor detects a voltage of
approximately - 120 mV across the damper connector.

Probable cause Suggested actions

1 Damper disconnected. Please contact your Agilent service

representative.

2 Defective damper. Please contact your Agilent service

representative.

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 Error Information 7
Module Error Messages

Valve Failed
Error ID: 2040
Valve 0 Failed: valve A1
Valve 1 Failed: valve A2
Valve 2 Failed: valve B2
Valve 3 Failed: valve B1
One of the solvent selection valves in the module failed to switch
correctly.
The processor monitors the valve voltage before and after each switching
cycle. If the voltages are outside expected limits, the error message is
generated.

Probable cause Suggested actions

1 Solvent selection valve disconnected. Please contact your Agilent service

representative.

2 Connection cable (inside instrument) not Please contact your Agilent service
connected. representative.

3 Connection cable (inside instrument) Please contact your Agilent service

defective. representative.

4 Solvent selection valve defective. Exchange the solvent selection valve.

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7 Error Information
Module Error Messages

Missing Pressure Reading

Error ID: 2054
The pressure readings read by the pump ADC (analog- digital converter)
are missing.
The ADC reads the pressure signal of from the damper every 1ms. If the
readings are missing for longer than 10 s, the error message is generated.

Probable cause Suggested actions

1 Damper disconnected. Please contact your Agilent service

representative.

2 Defective damper. Please contact your Agilent service

representative.

3 Defective main board. Please contact your Agilent service

representative.

Wrong Pump Configuration

Error ID: 2060
At switch- on, the pump has recognized a new pump configuration.
The binary pump is assigned its configuration at the factory. If the active
inlet valve and pump encoder of channel B are disconnected, and the
binary pump is rebooted, the error message is generated.

Probable cause Suggested actions

1 Active-inlet valve and pump encoder of Please contact your Agilent service
channel B disconnected. representative.

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 Error Information 7
Module Error Messages

Electronic Fuse of SSV Open

Error ID: 2049
Valve Fuse 0: Channels A1 and A2
Valve Fuse 1: Channels B1 and B2
One of the solvent- selection valves in the module has drawn excessive
current causing the selection- valve electronic fuse to open.

Probable cause Suggested actions

1 Defective solvent selection valve. Restart the pump. If the error message appears
again, exchange the solvent selection valve.

2 Defective connection cable (front panel to Please contact your Agilent service
main board). representative.

3 Defective main board. Please contact your Agilent service

representative.

AIV Fuse
Error ID: 2044
Inlet- Valve Fuse 0: Pump channel A
Inlet- Valve Fuse 1: Pump channel B
One of the active- inlet valves in the module has drawn excessive current
causing the inlet- valve electronic fuse to open.

Probable cause Suggested actions

1 Defective active inlet valve. Restart the module. If the error message
appears again, exchange the active inlet valve.

2 Defective connection cable (front panel to Please contact your Agilent service
main board). representative.

3 Defective main board. Please contact your Agilent service

representative.

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7 Error Information
Module Error Messages

Temperature Out of Range

Error ID: 2517
Temperature Out of Range 0: Pump channel A
Temperature Out of Range 1: Pump channel B
One of the temperature sensor readings in the motor- drive circuit are out
of range.
The values supplied to the ADC by the hybrid sensors must be between
0.5 V and 4.3 V. If the values are outside this range, the error message is
generated.

Probable cause Suggested actions

1 Defective main board. Please contact your Agilent service

representative.

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 Error Information 7
Module Error Messages

Temperature Limit Exceeded

Error ID: 2517
Temperature Limit Exceeded 0: Pump channel A
Temperature Limit Exceeded 1: Pump channel B
The temperature of one of the motor- drive circuits is too high.
The processor continually monitors the temperature of the drive circuits
on the main board. If excessive current is being drawn for long periods,
the temperature of the circuits increases. If the temperature exceeds the
upper limit, the error message is generated.

Probable cause Suggested actions

1 High friction (partial mechanical blockage) Remove the pump-head assembly. Ensure there
in the pump drive assembly. is no mechanical blockage of the pump-head
assembly or pump drive assembly.

2 Partial blockage of the flowpath in front of Ensure the outlet valve is not blocked.
the damper.

3 Defective pump drive assembly. Please contact your Agilent service

representative.

4 Defective main board. Please contact your Agilent service

representative.

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7 Error Information
Module Error Messages

Motor-Drive Power
Error ID: 2041, 2042
Motor- Drive Power: Pump channel A
B: Motor- Drive Power: Pump channel B
The current drawn by the pump motor exceeded the maximum limit.
Blockages in the flow path are usually detected by the pressure sensor in
the damper, which result in the pump switching off when the upper
pressure limit is exceeded. If a blockage occurs before the damper, the
pressure increase cannot be detected by the pressure sensor and the
module will continue to pump. As pressure increases, the pump drive
draws more current. When the current reaches the maximum limit, the
module is switched off, and the error message is generated.

Probable cause Suggested actions

1 Flow path blockage in front of the damper. Ensure the capillaries and frits between the
pump head and damper inlet are free from
blockage.

2 Blocked active inlet valve. Exchange the active inlet valve.

3 Blocked outlet valve. Exchange the outlet valve.

4 High friction (partial mechanical blockage) Remove the pump-head assembly. Ensure there
in the pump drive assembly. is no mechanical blockage of the pump-head
assembly or pump drive assembly.

5 Defective pump drive assembly. Please contact your Agilent service

representative.

6 Defective main board. Please contact your Agilent service

representative.

7 Restriction capillary blocked at pre-mixing Exchange restriction capillary.

union.

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 Error Information 7
Module Error Messages

Encoder Missing
Error ID: 2046, 2050, 2510
Encoder Missing: Pump channel A
B: Encoder Missing: Pump channel B
The optical encoder on the pump motor in the module is missing or
defective.
The processor checks the presence of the pump encoder connector every
2 s. If the connector is not detected by the processor, the error message is
generated.

Probable cause Suggested actions

1 Defective or disconnected pump encoder Please contact your Agilent service

connector. representative.

2 Defective pump drive assembly. Please contact your Agilent service

representative.

Inlet-Valve Missing
Error ID: 2048, 2052
Inlet- Valve Missing: Pump channel A
B: Inlet- Valve Missing: Pump channel B
The active- inlet valve in the module is missing or defective.
The processor checks the presence of the active- inlet valve connector
every 2 s. If the connector is not detected by the processor, the error
message is generated.

Probable cause Suggested actions

1 Disconnected or defective cable. Ensure the pins of the active inlet valve
connector are not damaged. Ensure the
connector is seated securely.

2 Disconnected or defective connection cable Please contact your Agilent service

(front panel to main board). representative.

3 Defective active inlet valve. Exchange the active inlet valve.

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7 Error Information
Module Error Messages

Servo Restart Failed

Error ID: 2201, 2211
Servo Restart Failed: Pump channel A
B: Servo Restart Failed: Pump channel B
The pump motor in the module was unable to move into the correct
position for restarting.
When the module is switched on, the first step is to switch on the C
phase of the variable reluctance motor. The rotor should move to one of
the C positions. The C position is required for the servo to be able to take
control of the phase sequencing with the commutator. If the rotor is
unable to move, or if the C position cannot be reached, the error message
is generated.

Probable cause Suggested actions

1 Disconnected or defective cable. Please contact your Agilent service

representative.

2 Blocked active inlet valve. Exchange the active inlet valve.

3 Mechanical blockage of the module. Remove the pump-head assembly. Ensure there
is no mechanical blockage of the pump-head
assembly or pump drive assembly.

4 Defective pump drive assembly. Please contact your Agilent service

representative.

5 Defective main board. Please contact your Agilent service

representative.

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 Error Information 7
Module Error Messages

Pump Head Missing

Error ID: 2202, 2212
Pump Head Missing: Pump channel A
B: Pump Head Missing: Pump channel B
The pump- head end stop in the pump was not found.
When the pump restarts, the metering drive moves forward to the
mechanical end stop. Normally, the end stop is reached within 20 s,
indicated by an increase in motor current. If the end point is not found
within 20 s, the error message is generated.

Probable cause Suggested actions

1 Pump head not installed correctly (screws Install the pump head correctly. Ensure nothing
not secured, or pump head not seated (e.g. capillary) is trapped between the pump
correctly). head and body.

2 Broken piston. Exchange the piston.

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7 Error Information
Module Error Messages

Index Limit
Error ID: 2203, 2213
Index Limit: Pump channel A
B: Index Limit: Pump channel B
The time required by the piston to reach the encoder index position was
too short (pump).
During initialization, the first piston is moved to the mechanical stop.
After reaching the mechanical stop, the piston reverses direction until the
encoder index position is reached. If the index position is reached too
fast, the error message is generated.

Probable cause Suggested actions

1 Irregular or sticking drive movement. Remove the pump head, and examine the seals,
pistons, and internal components for signs of
wear, contamination or damage. Exchange
components as required.

2 Defective pump drive assembly. Please contact your Agilent service

representative.

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 Error Information 7
Module Error Messages

Index Adjustment
Error ID: 2204, 2214
Index Adjustment: Pump channel A
B: Index Adjustment: Pump channel B
The encoder index position in the module is out of adjustment.
During initialization, the first piston is moved to the mechanical stop.
After reaching the mechanical stop, the piston reverses direction until the
encoder index position is reached. If the time to reach the index position
is too long, the error message is generated.

Probable cause Suggested actions

1 Irregular or sticking drive movement. Remove the pump head, and examine the seals,
pistons, and internal components for signs of
wear, contamination or damage. Exchange
components as required.

2 Defective pump drive assembly. Please contact your Agilent service

representative.

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7 Error Information
Module Error Messages

Index Missing
Error ID: 2205, 2215, 2505
Index Missing: Pump channel A
B: Index Missing: Pump channel B
The encoder index position in the module was not found during
initialization.
During initialization, the first piston is moved to the mechanical stop.
After reaching the mechanical stop, the piston reverses direction until the
encoder index position is reached. If the index position is not recognized
within a defined time, the error message is generated.

Probable cause Suggested actions

1 Disconnected or defective encoder cable. Please contact your Agilent service

representative.

2 Defective pump drive assembly. Please contact your Agilent service

representative.

Stroke Length
Error ID: 2206, 2216
Stroke Length: Pump channel A
B: Stroke Length: Pump channel B
The distance between the lower piston position and the upper mechanical
stop is out of limits (pump).
During initialization, the module monitors the drive current. If the piston
reaches the upper mechanical stop position before expected, the motor
current increases as the module attempts to drive the piston beyond the
mechanical stop. This current increase causes the error message to be
generated.

Probable cause Suggested actions

1 Defective pump drive assembly. Please contact your Agilent service

representative.

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 Error Information 7
Module Error Messages

Initialization Failed
Error ID: 2207, 2217
Initialization Failed: Pump channel A
B: Initialization Failed: Pump channel B
The module failed to initialize successfully within the maximum time
window.
A maximum time is assigned for the complete pump- initialization cycle. If
the time is exceeded before initialization is complete, the error message is
generated.

Probable cause Suggested actions

1 Blocked active inlet valve. Exchange the active inlet valve.

2 Defective pump drive assembly. Please contact your Agilent service

representative.

3 Defective main board. Please contact your Agilent service

representative.

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7 Error Information
Module Error Messages

124 1260 Infinity Binary Pump User Manual

 1260 Infinity Binary Pump User Manual

8
Test Functions and Calibration
(System) Pressure Test 126
Positioning the Blank Nut 127
Running the (System) Pressure Test 128
Evaluating the Results 129
Valve Test 131
Running the Valve Test 132
Evaluating the Results 132
Binary Pump Solvent Compressibility Calibration 133
Running the Solvent Compressibility Calibration 134
Pump Elasticity Calibration 135
Running the Pump Elasticity Calibration 136
Leak Rate Test 137
Running the Test 139
Evaluating the Results 140
Potential Causes of Leak Rate Test Failure 141

This chapter explains all test functions that are available for the binary pump.

Agilent Technologies 125

8 Test Functions and Calibration
(System) Pressure Test

(System) Pressure Test

This Lab Advisor test uses different names depending on the firmware revision used:
NOTE
• FW revision ≥ A.06.50: System Pressure Test
• FW revision < A.05.60: Pressure Test

Description
The (system) pressure test is a quick built- in test designed to demonstrate
the leak tightness of the system. The test involves monitoring the flow
profile while the pump delivers against a blank nut. The result is
presented as the leak rate of the module and provides information about
the leak tightness of the system between the outlet valves of the pump
and the blank nut.

The blank nut can be positioned anywhere between the purge valve of the pump and the
NOTE detector inlet to pressure test the desired part of the system.

Blank nut placed at the outlet of flow cell

CAUTION
The applied pressure may cause permanent leaks or bursting of the flow cell.
➔ Never include the flow cell in the pressure test.

Step 1
The test begins with the initialization of both pump heads. After
initialization, the pump is starting the compression phase and the required
flow rate is constantly monitored and adjusted. The pump continues to
pump until a system pressure of around 600 bar is reached.

Step 2
When the system pressure reaches 600 bar, the pump continues to pump
at a flow rate that keeps the pressure constant. The flow that is needed to
keep the pressure constant is directly translated into a leak rate.

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 Test Functions and Calibration 8
(System) Pressure Test

Positioning the Blank Nut

To test the complete system’s pressure tightness, the blank nut should be
positioned at the column compartment outlet (or the outlet of the last
module before the detector).
If a specific component is suspected of causing a system leak, place the
blank nut immediately before the suspected component, and then run the
(System) Pressure Test again. If the test passes, the defective component is
located after the blank nut. Confirm the diagnosis by placing the blank
nut immediately after the suspected component. The diagnosis is
confirmed if the test fails.

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8 Test Functions and Calibration
(System) Pressure Test

Running the (System) Pressure Test

Running the test from the Agilent Lab Advisor

When The test should be used when problems with small leaks are suspected, or after maintenance of
flow path components (e.g., pump seals, injection seal) to prove pressure tightness up to 600 bar

Tools required p/n Description

8710-0510 Wrench, 1/4 - 5/16 inch, open
Blank nut 1/16 inch

Preparations Place two bottles of HPLC-grade water in channels A and B (A1 and B1 if the pump is equipped with
a solvent selection valve)

Make absolutely sure that all parts of the flow path that are part of the test are very
NOTE thoroughly flushed with water before starting to pressurize the system! Any trace of other
solvents or the smallest air bubble inside the flow path definitely will cause the test to fail!

1 Select the (system) pressure test from the test selection menu.
2 Start the test and follow the instructions.

Make sure to release the pressure by opening the purge valve when the test has
NOTE completed. Otherwise the pump may generate an overpressure error.

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 Test Functions and Calibration 8
(System) Pressure Test

Evaluating the Results

The sum of all leaks between the pump and the blank nut will add up to
the total leak rate. Note that small leaks may cause the test to fail, but
solvent may not be seen leaking from a module.

Please notice the difference between an error in the test and a failure of the test! An error
NOTE is caused by the abnormal termination during the operation of the test whereas a failure of
a test indicates that the test results were not within the specified limits.

If the pressure test fails:

• Ensure all fittings between the pump and the blank nut are tight.
Repeat the pressure test.

Often it is only a damaged blank nut itself (poorly shaped from overtightening) that causes
NOTE the test to fail. Before investigating on any other possible sources of failure make sure that
the blank nut you are using is in good condition and properly tightened!

• If the test fails again, insert the blank nut at the outlet of the previous
module in the stack (e.g. autosampler, port 6 of the injection valve), and
repeat the pressure test. Exclude each module one by one to determine
which module is leaking.
• If the pump is determined to be the source of the leak, run the valve
test to identify the defective pump component.

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8 Test Functions and Calibration
(System) Pressure Test

Potential Causes of Pressure Test Failure

After isolating and fixing the cause of the leak, repeat the pressure test to
confirm the system is pressure tight.

Table 9 Potential Cause (Pump)

Potential Cause (Pump) Corrective Action

Purge valve open. Close the purge valve.

Loose or leaky fitting. Tighten the fitting or exchange the capillary.

Damaged pump seals or pistons. Run the valve test to identify the defective
component.

Loose purge valve. Tighten the purge valve nut (14 mm wrench).

Table 10 Potential Cause (Autosampler)

Potential Cause (Autosampler) Corrective Action

Loose or leaky fitting. Tighten or exchange the fitting or capillary.

Rotor seal (injection valve). Exchange the rotor seal.

Damaged metering seal or piston. Exchange the metering seal. Check the piston
for scratches. Exchange the piston if required.

Needle seat. Exchange the needle seat.

Table 11 Potential Cause (Column Compartment)

Potential Cause (Column Compartment) Corrective Action

Loose or leaky fitting. Tighten or exchange the fitting or capillary.

Rotor seal (column switching valve). Exchange the rotor seal.

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 Test Functions and Calibration 8
Valve Test

Valve Test

Description
The Valve Test provides a fast and accurate way to verify proper hydraulic
operation of the binary pump. Problems that are related to defective
valves, seals or pistons can be diagnosed and usually the defective part is
identified.

If a minimum firmware of A.06.50 is installed on this module and a minimum Lab Advisor
NOTE revision of B.02.03. is available, please run the improved Leak Rate Test instead (see “Leak
Rate Test” on page 137).

Step 1
The system is setup with water on both channels and a restriction
capillary is attached to the outlet of the pump. Pump head A is delivering
at 1 mL/min. The pressure signal is monitored and overlaid with the
piston movement plot. The pressure pattern and the slope of the pressure
signal are evaluated for the delivery strokes of both pistons.

Step 2
The procedure from step 1 is repeated on pump head B.

Step 3
The data from step 1 and 2 are evaluated. In case test failed, a conclusion
about the defective part is made.

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8 Test Functions and Calibration
Valve Test

Running the Valve Test

Running the test from the Agilent Lab Advisor

When • For FW revisions < A.06.50

• The test should be used to prove proper operation of the binary pump after repairs or when the
pressure test (see “(System) Pressure Test” on page 126) determined a problem with the pump.

Tools required p/n Description

8710-0510 Wrench, 1/4 - 5/16 inch, open

Parts required p/n Description

G1312-67500 Calibration capillary assembly

Preparations Place two bottles of HPLC-grade water in channels A and B (A1 and B1 if the pump is equipped with
a solvent selection valve)

The Valve Test should only be performed when firmware revisions < A.06.50 are installed.
NOTE
For firmware revision A.06.50 or above, perform the Leak Rate Test instead.

Make absolutely sure that the pump is very thoroughly flushed with water before starting
NOTE the test! Any trace of other solvents or the smallest air bubble inside the flow path
definitely will cause the test to generate misleading results!

1 Select the valve test from the test selection menu.

2 Start the test and follow the instructions.

Make sure to release the pressure by opening the purge valve when the test has
NOTE completed. Otherwise the pump may generate an overpressure error.

Evaluating the Results

Refer to the help file of the Agilent Lab Advisor for further details.

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 Test Functions and Calibration 8
Binary Pump Solvent Compressibility Calibration

Binary Pump Solvent Compressibility Calibration

Description
Each solvent or solvent mixture has unique compressibility at different
pressures. In order to deliver accurate flow with minimal pressure- and
composition ripple over the full operational pressure range, it is necessary
that the pump compensates precisely for the compressibility of the
solvents in use.
The binary pump comes with compressibility parameters for the most
common HPLC solvents and solvent mixtures. If a solvent is not available
in the list of pre- calibrated solvents, the solvent compressibility calibration
allows the appropriate compressibility data to be generated.

Technical background
The solvent compressibility calibration relies on an accurate elasticity
calibration of the pump. With a proper elasticity calibration in place, the
pump is switched into pressure control mode. A restriction capillary is
connected to the purge valve outlet. By varying the flow rate, the pump
maintains a certain pressure. The pump optimizes the compressibility
value of the solvent until the lowest possible pump ripple is reached. The
pump increases the flow rate and adjusts the pressure to the next
calibration step where the pump ripple is minimized again. This process is
repeated until solvent compressibility data for the whole operating
pressure range of the pump are available.
The compressibility data set for this solvent is stored in an XML- file in
C:\Documents and Settings\<username>\Application Data\Agilent Technologies\Agilent
Lab Advisor\2.02.0.0\data\. It can be shared with other G1312B pumps via
the controlling data system.

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8 Test Functions and Calibration
Binary Pump Solvent Compressibility Calibration

Running the Solvent Compressibility Calibration

Running the Solvent Compressibility Calibration from the Agilent Lab
Advisor

When If a solvent is not available in the list of pre-calibrated solvents, the solvent compressibility
calibration allows to generate appropriate compressibility data.

Tools required p/n Description

8710-0510 Wrench, 1/4 - 5/16 inch, open

Parts required p/n Description

G1312-67500 Calibration capillary assembly

Preparations Place a bottles with solvent to be calibrated in channel A (resp. A1 if a solvent selection valve is
installed).

Avoid inaccurate pump elasticity calibration.

CAUTION
This would lead into invalid and not-portable solvent compressibility data.
➔ Make sure to perform accurate pump elasticity calibration.

Make absolutely sure that the pump is very thoroughly flushed with the solvent to be
NOTE calibrated before starting the procedure! Any trace of other solvents or the smallest air
bubble inside the flow path definitely will cause the calibration to fail!

1 Select the solvent from the test selection menu.

2 Start the test and follow the instructions.

Make sure to release the pressure by opening the purge valve when the test has
NOTE completed. Otherwise the pump may generate an overpressure error.

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 Test Functions and Calibration 8
Pump Elasticity Calibration

Pump Elasticity Calibration

Description
The flow path components of the binary pump have an inherent and
pressure dependent elasticity which differs from pump to pump. When
this elasticity/pressure function is known, a correction algorithm can be
applied. This results in significantly improved pump performance in low
delay volume mode (damper and mixer bypassed).
The pump elasticity calibration uses a solvent with well known properties
(HPLC- grade water) to determine the pump elasticity over the entire
operating pressure range and stores the calibration values in the
non- volatile RAM of the pump mainboard.
The initial calibration is done at the factory. It only needs to be repeated
after replacement of major pump parts (mainboard, pump drive). The test
allows to define which pump head will be calibrated.

Results of the pump elasticity calibration rely on known compressibility parameters for pure
NOTE water. If the water is not HPLC-grade, not well degassed or degasser and pump are not
flushed properly, the pump elasticity calibration will fail. The pump elasticity calibration has
to be performed for each pump head individually.

Incorrect pump elasticity calibration.

CAUTION
Solvent compressibility calibrations acquired with a miscalibrated pump will work, but
they are not transferable to other pumps. A correct pump elasticity calibration is an
essential prerequisite for successful solvent compressibility calibrations.
➔ Calibrate the pump elasticity correctly.

1260 Infinity Binary Pump User Manual 135

8 Test Functions and Calibration
Pump Elasticity Calibration

Running the Pump Elasticity Calibration

Running the Pump Elasticity Calibration from the Agilent Lab Advisor
Software

When The initial calibration is done at the factory. It only needs to be repeated after replacement of major
pump parts (mainboard, pump drive).

Tools required p/n Description

8710-0510 Wrench, 1/4 - 5/16 inch, open

Parts required p/n Description

G1312-67500 Calibration capillary assembly

Preparations Place all bottle heads in to a bottle of HPLC-grade water.

Make absolutely sure that the pump is very thoroughly flushed with the solvent to be
NOTE calibrated before starting the procedure! Any trace of other solvents or the smallest air
bubble inside the flow path definitely will cause the calibration to fail!

If a solvent selection valve is installed flush all four solvent channels to avoid that air from a
NOTE dry solvent intake tube is drawn into the flow path upon initialization.

1 Select the pump elasticity calibration from the test selection menu.
2 Start the test and follow the instructions.

Make sure to release the pressure by opening the purge valve when the test has
NOTE completed. Otherwise the pump may generate an overpressure error.

136 1260 Infinity Binary Pump User Manual

 Test Functions and Calibration 8
Leak Rate Test

Leak Rate Test

Introduction
The Leak Rate Test is used for verifying the internal tightness of the pump
and helps identifying parts which may have caused a leak.

System requirements
Minimum software revisions:
• Lab Advisor B.02.03
Minimum firmware revisions:
• A.06.50

This test does not work in emulation mode, that is when the G1312B Binary Pump emulates
NOTE the G1312A Binary Pump. In case of an emulated module, convert to the original type first
(see “Replacing Module Firmware” on page 181).

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8 Test Functions and Calibration
Leak Rate Test

Test Principle
A solvent can be chosen from available solvent channels and a maximum
target pressure can be defined at which the test will be run. Typically,
this is the maximum pressure specified for the pump. The test can be run
with any solvent compatible to the pump.
Before the test, the pump is flushed with solvent in order to remove air
bubbles, as air bubbles are compressed during the test and therefore
would appear as leaks. Using a degasser is highly recommended.

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Initially, the pressure is increased to about 100 bar below the target
pressure, which has been set for the test.
Then piston 1 is brought to its rear position. An increasing flow is
delivered by piston 1. In case of a leak, the pressure will drop initially as
long as the flow rate delivered by the piston is lower than the leak rate.
As soon as the flow rate of the piston exceeds the leak rate, the measured
pressure will increase again. Therefore the minimum pressure of that

138 1260 Infinity Binary Pump User Manual

 Test Functions and Calibration 8
Leak Rate Test

curve segment corresponds to the flow and leak rate at that time and the
leak rate is measured.
Subsequently, piston 2 is moved to its rear position, then piston 2 delivers
and the measurement is done as described for piston 1.
For a binary pump (G1312B/C, K1312B), the test is run for both pump
heads for channels A and B.

Running the Test

Parts required p/n Description

01080-83202 Blank nut

Running the test from the Agilent Lab Advisor

1 Select the Leak Rate Test from the Test Selection menu.
2 Start the test and follow the instructions.

Make sure to release the pressure by slowly opening the purge valve when the test has
NOTE finished.

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8 Test Functions and Calibration
Leak Rate Test

Evaluating the Results

Results of the leak rate test are the leak rates measured for pistons 1 and
2 as described for the test principle. If any of the leak rates exceeds
3 µL/min, the test will fail.

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140 1260 Infinity Binary Pump User Manual

 Test Functions and Calibration 8
Leak Rate Test

Potential Causes of Leak Rate Test Failure

For binary pumps, secondary pump chambers are connected. A leak observed in any of
NOTE these chambers may be caused by the other secondary pump chamber.

Secondary Leak
If a leak is found for movement of piston 2 (secondary leak), the following
reasons are possible:

Probable cause Suggested actions

1 System not flushed properly Flush system for several minutes

2 Degassing efficiency is low Check degasser performance

3 Purge valve not closed or defect Check purge valve

4 Blank nut not installed tightly Tighten or replace blank nut

5 Outlet valve leaking (read below) Replace outlet valve

6 Leak at piston 2 or seal in chamber 2 Inspect piston, replace piston and/or seal

Primary Leak
If a leak is found for movement of piston 1 (primary leak), any leak
described for piston movement 2 will cause a failure for piston 1 as well,
as the liquid can move through the outlet valve to chamber 2. Such cases
need to be identified as described before. Additionally, following causes
are possible:

Probable cause Suggested actions

1 Leak at piston 1 or seal in chamber 1 Inspect piston, replace piston and/or seal

2 Leak at inlet valve Replace inlet valve or inlet valve cartridge (AIV only)

Internal Outlet Valve Leak

A leak of the outlet valve will be identified separately (internal outlet
valve leak) by calculating the difference between leak rate 1 and leak rate
2. If the second leak rate is higher than the first one, this is due to a flow
back through the outlet valve.

Probable cause Suggested actions

1 Leak at outlet valve Replace the part which has failed and re-run the test.

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8 Test Functions and Calibration
Leak Rate Test

142 1260 Infinity Binary Pump User Manual

 1260 Infinity Binary Pump User Manual

9
Diagnostic Signals
Analog Pressure Output 144
Diagnostic Signals in Chromatographic Data Systems 145
Instrument Signals 145

This chapter explains all diagnostic signals of the binary pump.

Agilent Technologies 143

9 Diagnostic Signals
Analog Pressure Output

Analog Pressure Output

A BNC connector at the rear of the binary pump provides the reading of
the pressure sensor as analog value with a resolution of 1.33 mV/bar. The
maximum reading of 660 bar equals 800 mV. The signal is available in
real time and can be fed into an appropriate recording device (e.g.
integrator or strip chart recorder) for troubleshooting purposes.

Analog pressure
output

Figure 22 Location of analog output connector

144 1260 Infinity Binary Pump User Manual

 Diagnostic Signals 9
Diagnostic Signals in Chromatographic Data Systems

Diagnostic Signals in Chromatographic Data Systems

Instrument Signals
In Chromatographic Data Systems (for example, Agilent OpenLAB CDS, or
any 3rd party software that supports the ICF (Instrument Control
Framework) standard), the following instrument parameters are accessible
during data acquisition and can be stored in the data file:
• current pump pressure
• solvent composition (gradient)

1260 Infinity Binary Pump User Manual 145

9 Diagnostic Signals
Diagnostic Signals in Chromatographic Data Systems

146 1260 Infinity Binary Pump User Manual

 1260 Infinity Binary Pump User Manual

10
Maintenance
Introduction to Maintenance and Repair 148
Warnings and Cautions 149
Cleaning the Module 151
Overview of Maintenance and Simple Repairs 152
Maintenance Procedures 153
Exchanging the Purge Valve Frit or the Purge Valve 154
Removing the Pump Head Assembly 157
Maintenance of a Pump Head without Seal Wash 159
Maintenance of a Pump Head with Seal Wash 163
Reinstalling the Pump Head Assembly 167
Seal Wear-in Procedure 169
Exchanging the Active Inlet Valve (AIV) or its Cartridge 170
Exchanging the Outlet Valve 173
Installation of the Solvent Selection Valve Upgrade Kit 175
Exchanging the Solvent Selection Valve 177
Exchanging the Optional Interface Board 180
Replacing Module Firmware 181

This chapter describes the maintenance of the module.

Agilent Technologies 147

10 Maintenance
Introduction to Maintenance and Repair

Introduction to Maintenance and Repair

The pump is designed for easy repair. The most frequent repairs such as
piston seal replacement and purge valve frit exchange can be done from
the front side without removing the pump from the system stack. These
repairs are described in “Overview of Maintenance and Simple Repairs” on
page 152 .

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 Maintenance 10
Warnings and Cautions

Warnings and Cautions

Toxic, flammable and hazardous solvents, samples and reagents

WA R N I N G
The handling of solvents, samples and reagents can hold health and safety risks.
➔ When working with these substances observe appropriate safety procedures (for
example by wearing goggles, safety gloves and protective clothing) as described in
the material handling and safety data sheet supplied by the vendor, and follow good
laboratory practice.
➔ The volume of substances should be reduced to the minimum required for the
analysis.
➔ Do not operate the instrument in an explosive atmosphere.

Electrical shock
WA R N I N G
Repair work at the module can lead to personal injuries, e.g. shock hazard, when the
cover is opened.
➔ Do not remove the cover of the module.
➔ Only certified persons are authorized to carry out repairs inside the module.

Personal injury or damage to the product

WA R N I N G
Agilent is not responsible for any damages caused, in whole or in part, by improper
use of the products, unauthorized alterations, adjustments or modifications to the
products, failure to comply with procedures in Agilent product user guides, or use of
the products in violation of applicable laws, rules or regulations.
➔ Use your Agilent products only in the manner described in the Agilent product user
guides.

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10 Maintenance
Warnings and Cautions

Safety standards for external equipment

CAUTION
➔ If you connect external equipment to the instrument, make sure that you only use
accessory units tested and approved according to the safety standards appropriate
for the type of external equipment.

Agilent 1260 Infinity pump heads have been discontinued for Agilent 1260 Infinity
CAUTION
Binary Pumps
A number of 1260 Infinity Binary Pumps have been shipped with pump heads labeled
as 1260 Infinity pump heads and passive inlet valves. While these parts are as good
from a performance perspective, they are no longer used for 1260 Infinity Binary
Pumps. Parts listed in this manual are not compatible to 1260 Infinity pump heads and
may get damaged.
➔ Please contact your Agilent service representative.

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 Maintenance 10
Cleaning the Module

Cleaning the Module

To keep the module case clean, use a soft cloth slightly dampened with
water, or a solution of water and mild detergent.

Liquid dripping into the electronic compartment of your module can cause shock
WA R N I N G
hazard and damage the module
➔ Do not use an excessively damp cloth during cleaning.
➔ Drain all solvent lines before opening any connections in the flow path.

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10 Maintenance
Overview of Maintenance and Simple Repairs

Overview of Maintenance and Simple Repairs

Figure 23 on page 152 shows the main user accessible assemblies of the
binary pump. The pump heads and its parts require normal maintenance
(for example, seal exchange) and can be accessed from the front (simple
repairs). Replacement of valve cartridges or filters don’t require to remove
the pump from the system stack.

Purge valve

Outlet valve

Active inlet valve

Solvent selection valve

Pump head

Figure 23 Overview of Maintenance and Simple Repairs

1 Purge valve, see “Exchanging the Purge Valve Frit or the Purge Valve” on page 154

2 Outlet valve, see “Exchanging the Outlet Valve” on page 173

3 Active inlet valve, see “Exchanging the Active Inlet Valve (AIV) or its Cartridge” on
page 170

4 Pump head, see “Removing the Pump Head Assembly” on page 157

5 Solvent selection valve, see “Installation of the Solvent Selection Valve Upgrade Kit” on
page 175

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 Maintenance 10
Maintenance Procedures

Maintenance Procedures

The procedures described in this section can be done with the binary
pump in place in the system stack.

Table 12 Maintenance procedures

Procedure Typical Frequency Notes

“Exchanging the Purge Valve Frit or the Yearly, or if the frit shows A pressure drop of > 10 bar in low delay volume
Purge Valve” on page 154 indication of configuration and > 20 bar in standard
contamination or configuration across the frit (5 mL/min H 2O
blockage with purge valve open) indicates blockage
If internally leaking Solvent dripping out of waste outlet when valve
is closed

“Removing the Pump Head Assembly” on During yearly Necessary to get access to pump seals and
page 157 maintenance pistons

“Maintenance of a Pump Head without Seal Yearly, or if pump Leaks at lower pump head side, unstable
Wash” on page 159 performance indicates retention times, pressure ripple unstable — run
seal wear Valve Test for verification
Seal life time shorter than normally expected —
check pistons while changing the seals

“Maintenance of a Pump Head with Seal Yearly, or if pump Only necessary when Seal Wash Option is
Wash” on page 163 performance indicates installed. Leaks at lower pump head side, loss
seal wear of wash solvent

“Exchanging the Active Inlet Valve (AIV) or If leaking externally Error messages “Inlet Valve Fuse” or “Inlet
its Cartridge” on page 170 If solenoid is defective Valve Missing”

“Exchanging the Outlet Valve” on page 173 If internally leaking Pressure ripple unstable, run Valve Test for
verification

“Exchanging the Solvent Selection If internally leaking Cross port flow

Valve” on page 177 If solenoid is defective Error message “Valve Failed”

1260 Infinity Binary Pump User Manual 153

10 Maintenance
Exchanging the Purge Valve Frit or the Purge Valve

Exchanging the Purge Valve Frit or the Purge Valve

When • Frit – when piston seals are exchanged or when contaminated or blocked (pressure drop of
> 10 bar in low delay volume configuration and > 20 bar in standard configuration across the frit
at a flow rate of 5 mL/min of water with purge valve opened)
• Purge valve – if internally leaking

Tools required p/n Description

8710-0510 Wrench open 1/4 — 5/16 inch
8710-1924 Wrench open 14 mm
Pair of tweezers
OR Toothpick

Parts required # p/n Description

1 01018-22707 PTFE frits (pack of 5)
1 G1312-60061 Purge valve 1260
1 5067-4728 Seal cap (OPTIONAL)

Preparations • Switch off pump at the main power switch

• Remove the front cover
• Use an optional solvent shutoff valve or lift up solvent filters in solvent reservoirs for avoiding
leakages.

154 1260 Infinity Binary Pump User Manual

 Maintenance 10
Exchanging the Purge Valve Frit or the Purge Valve

1 Using a 1/4 inch wrench disconnect the pump outlet capillary from the
purge valve. Disconnect the waste tube. Beware of leaking solvents due
to hydrostatic pressure.

EjbedjiaZiXVe^aaVgn

Ejg\ZkVakZ

LVhiZijWZ

2 Using the 14 mm wrench, unscrew the purge valve and remove it from
the purge valve holder.
3 Remove the seal cap from the purge valve.
4 Using a pair of tweezers or a toothpick remove the frit.

KVakZWdYn

EI;:[g^il^i]\gddkZ

HZVaXVe

1260 Infinity Binary Pump User Manual 155

10 Maintenance
Exchanging the Purge Valve Frit or the Purge Valve

5 Place a new frit into the purge valve with the orientation of the frit as
shown below (slit in frit points to the front). Reinstall the seal cap
including the gold seal.

Before reinstallation always check the gold seal in the seal cap. A deformed seal cap
NOTE should be exchanged.

6 Insert the purge valve into the purge valve holder and orient the waste
outlet nozzle downward.
7 Tighten the purge valve and reconnect outlet capillary and waste
tubing.

156 1260 Infinity Binary Pump User Manual

 Maintenance 10
Removing the Pump Head Assembly

Removing the Pump Head Assembly

When • Exchanging pump seals

• Exchanging pistons
• Exchanging seals of the seal wash option

Tools required p/n Description

8710-0510 Wrench open 1/4 — 5/16 inch
8710-2411 Hex key 3 mm12 cm long
8710-2392 Hex key 4 mm15 cm long T-handle
5023-0240 Hex driver, ¼", slitted

Preparations Switch off the pump at the main power switch

Damage of the pump drive

CAUTION
Starting the pump when the pump head is removed may damage the pump drive.
➔ Never start the pump when the pump head is removed.

Both pump head assemblies use the same internal components. In addition, pump head A
NOTE is fitted with the purge valve. The following procedure describes the removal and
disassembly of pump head A (left). For pump head B (right) proceed in the same way and
skip steps that deal with the purge valve.

1 Remove the front cover.

2 Disconnect the capillaries at the back of the purge valve holder, the
pump head adapter and the tube at the active inlet valve. Beware of
leaking solvents.

1260 Infinity Binary Pump User Manual 157

10 Maintenance
Removing the Pump Head Assembly

3 Using a 4- mm hexagonal key stepwise loosen and remove the two pump
head screws.

Ejbe]ZVYhXgZlh

158 1260 Infinity Binary Pump User Manual

 Maintenance 10
Maintenance of a Pump Head without Seal Wash

Maintenance of a Pump Head without Seal Wash

When In case of maintenance or pump head internal leaks

Tools required p/n Description

8710-0510 Wrench open 1/4 — 5/16 inch
8710-2411 Hex key 3 mm12 cm long
8710-2392 Hex key 4 mm15 cm long T-handle
01018-23702 Insert tool

Parts required # p/n Description

1 5063-6589 Piston seal PTFE, carbon filled, black (pack of 2), default
OR 1 0905-1420 PE seals (pack of 2)
1 5063-6586 Sapphire piston

Preparations • Switch off the pump at the main power switch

• Remove the front cover to have access to the pump heads
• “Removing the Pump Head Assembly” on page 157

Both pump head assemblies use the same internal components. In addition, pump head A
NOTE is fitted with the purge valve. The following procedure describes the removal and
disassembly of pump head A (left). For pump head B (right) proceed in the same way and
skip steps that deal with the purge valve.

1260 Infinity Binary Pump User Manual 159

10 Maintenance
Maintenance of a Pump Head without Seal Wash

1 Place the pump head on a flat surface. Loosen the lock 2 Remove the support rings from the piston housing and lift
screw (two turns) and while holding the lower half of the the housing away from the pistons.
assembly (piston housing) carefully lift up the pump
housing and remove it from the piston housing.
Lock screw

Pump housing Hjeedgig^c\

E^hidc]djh^c\

Piston housing
E^hidc

160 1260 Infinity Binary Pump User Manual

 Maintenance 10
Maintenance of a Pump Head without Seal Wash

3 Check the piston surface and remove any deposits or 4 Using the steel side of the insert tool carefully remove
layers. Most suitable is polishing of the piston rod with the seal from the pump housing. Remove wear retainers,
toothpaste. Replace the piston if scratched or if dents are if still present.
visible.

Insert tool

Piston surface

Piston seal

Pump housing

NOTE
The best way to inspect a piston is to hold it up and
watch e.g. a light bulb through the piston rod. The
transparent sapphire acts as a very strong magnifier and
even smallest surface abnormalities become visible.
5 Using the plastic side of the insert tool insert the new 6 Place a seal wash gasket in the recess of the support
seals into the pump head and press them firmly in ring. Put the seal holder on top of the gasket.
position.

>chZgiidda Seal holder

Gasket

E^hidchZVah Support ring

1260 Infinity Binary Pump User Manual 161

10 Maintenance
Maintenance of a Pump Head without Seal Wash

7 Reassemble the pump head assembly. Note the correct 8 Insert the pistons and carefully press them into the seals.
position of the pin on the support ring.
E^hidc

Ejbe]djh^c\

Hjeedgig^c\h

E^hidc]djh^c\

NOTE
Reset the seal wear counter and liquimeter in the
Agilent Lab Advisor.
9 Tighten the lock screw. Next Steps:
10 Reinstall the pump head assembly, see “Reinstalling the
AdX`hXgZl Pump Head Assembly” on page 167.
11 If a standard seal has been installed, run the seal wear-in
procedure, see “Seal Wear-in Procedure” on page 169.
12 For the normal phase seal, the purge valve frit should be
replaced, see “Exchanging the Purge Valve Frit or the
Purge Valve” on page 154.

162 1260 Infinity Binary Pump User Manual

 Maintenance 10
Maintenance of a Pump Head with Seal Wash

Maintenance of a Pump Head with Seal Wash

When In case of maintenance or pump head internal leaks

Tools required p/n Description

8710-2411 Hex key 3 mm12 cm long
8710-2392 Hex key 4 mm15 cm long T-handle
01018-23702 Insert tool
Screwdriver, small flat head

Parts required p/n Description

0905-1175 Wash seal (PTFE)
5062-2484 Gasket, seal wash (pack of 6)
5063-6586 Sapphire piston

Preparations • Switch off the pump at the main power switch

• Remove the front cover to have access to the pump heads
• “Removing the Pump Head Assembly” on page 157

Both pump head assemblies use the same internal components. In addition, pump head A
NOTE is fitted with the purge valve. The following procedure describes the removal and
disassembly of pump head A (left). For pump head B (right) proceed in the same way and
skip steps that deal with the purge valve.

1260 Infinity Binary Pump User Manual 163

10 Maintenance
Maintenance of a Pump Head with Seal Wash

1 Place the pump head on a flat surface. Loosen the lock 2 Remove the seal holder and the seal wash support rings
screw (two turns) and while holding the lower half of the from the piston housing. Remove the seal holder from the
assembly (piston housing) carefully lift up the pump support ring assembly.
housing and remove it from the piston housing.
AdX`hXgZl
Seal holder

Seal wash support ring

Ejbe]djh^c\

Piston housing

E^hidc]djh^c\

Piston

3 Check the piston surface and remove any deposits or layers. 4 Using the steel side of the insert tool carefully remove
Most suitable is polishing of the piston rod with toothpaste. the seal from the pump housing. Remove wear retainers,
Replace the piston if scratched or if dents are visible. if still present.

Insert tool

Piston surface

Piston seal

Pump housing

NOTE
The best way to inspect a piston is to hold it up and
watch e.g. a light bulb through the piston rod. The
transparent sapphire acts as a very strong magnifier and
even smallest surface abnormalities become visible.
164 1260 Infinity Binary Pump User Manual
 Maintenance 10
Maintenance of a Pump Head with Seal Wash

5 Using the plastic side of the insert tool insert the new 6 Using the steel side of the insert tool remove the seal
seals into the pump head and press them firmly in wash gasket and the wash seal from the support ring.
position. The removed seal will be damaged and cannot be
re-used!

>chZgiidda

E^hidchZVah

Seal wash support ring

Wash seal

7 Using the plastic side of the insert tool press the new 8 Place a seal wash gasket in the recess of the support
wash seal (spring pointing upwards) into the recess of ring. Put the seal holder on top of the gasket.
the support ring.

Seal holder
>chZgiidda

Seal wash gasket

Support ring
LVh]hZVa

Hjeedgig^c\

1260 Infinity Binary Pump User Manual 165

10 Maintenance
Maintenance of a Pump Head with Seal Wash

9 Place the support rings on the piston housing (pistons 10 Insert the pistons and carefully press them into the seals.
not installed) and snap the pump housing and piston
housing together. Note the correct position of the pin on
the support ring.
E^hidc

Pump housing

Support ring

Piston housing

11 Tighten the lock screw. 12 Reinstall the pump head assembly, see “Reinstalling the
Pump Head Assembly” on page 167.
AdX`hXgZl

166 1260 Infinity Binary Pump User Manual

 Maintenance 10
Reinstalling the Pump Head Assembly

Reinstalling the Pump Head Assembly

When When reassembling the pump

Tools required p/n Description

8710-2411 Hexagonal key, 3 mm
Hexagonal key, 4 mm

Parts required # p/n Description

1 79846-65501 Pump head grease

1 Slide the pump head assembly onto the pump drive.

Ejg\ZkVakZ]daYZg

Ejbe]ZVYhXgZlh

2 Using a 4 mm hexagonal key tighten the pump head screws stepwise

with increasing torque.

1260 Infinity Binary Pump User Manual 167

10 Maintenance
Reinstalling the Pump Head Assembly

3 Reconnect the tubing and capillaries to the connector.

168 1260 Infinity Binary Pump User Manual

 Maintenance 10
Seal Wear-in Procedure

Seal Wear-in Procedure

Seal damage
CAUTION
This procedure is required for black PTFE seals (standard applications, p/n 5063-6589),
but it will damage the yellow PE seals (normal phase applications, p/n 0905-1420).
➔ Do not run the seal wear-in procedure if PE seals are installed in the pumphead.

1 Put a bottle with 100 ml of isopropanol in the solvent cabinet and place
the solvent intake filter of the pump head you want to wear in into this
bottle.
2 Screw the PEEK adapter 1/4- 28 to 10- 32 (0100- 1847) onto the active
inlet valve and connect the inlet tube from the bottle head directly to it.
3 Connect the Restriction capillary (5022- 2159) to the purge valve.
Connect its other end to a waste container.
4 Open the purge valve and purge the system for 5 min with isopropanol
at a flow rate of 2 mL/min.
5 Close the purge valve, set the flow to a value that gives a pressure of
350 bar. Pump 15 min at this pressure to wear the seals in. The
pressure can be monitored on the analog output connector of the pump,
with the Instant Pilot, chromatographic data system or any other
controlling device connected to your pump.
6 Turn OFF the pump, slowly open the purge valve to release the
pressure from the system, disconnect the restriction capillary and
reconnect the outlet capillary to the purge valve. Reconnect the intake
tubing to the solvent selection valve and the connecting tube from the
solvent selection valve (if installed) to the AIV.
7 Purge your system with the solvent used for your next application.

1260 Infinity Binary Pump User Manual 169

10 Maintenance
Exchanging the Active Inlet Valve (AIV) or its Cartridge

Exchanging the Active Inlet Valve (AIV) or its Cartridge

When If internally leaking (backflow)

Tools required Description

Wrench, 14 mm

Parts required p/n Description

G1312-60025 Active inlet valve body, without cartridge
G1312-60020 Cartridge for active inlet valve 600 bar

Preparations Switch off the pump at the main power switch

Ensure correct fit of the active inlet valve

CAUTION
Overtightening will destroy the active inlet valve cartridge.
➔ Tighten the active inlet valve properly.

1 Remove the front cover.

2 Unplug the active inlet valve cable from the connector.
3 Disconnect the solvent inlet tube at the inlet valve (beware of leaking
solvents).

Binary pumps without solvent selection valve (SSV) have an adapter installed between the
NOTE solvent line and the active inlet valve (AIV). Disconnect the solvent tubes at the adapter
and remove the adapter from the AIV.

170 1260 Infinity Binary Pump User Manual

 Maintenance 10
Exchanging the Active Inlet Valve (AIV) or its Cartridge

4 Using a 14 mm wrench, loosen the active inlet valve and remove the
valve from the pump head.

8dccZXidg

HdakZci^ciV`ZijW^c\

6Xi^kZ^caZikVakZ

5 Using a pair of tweezers, remove the valve cartridge from the defective
active inlet valve.

1260 Infinity Binary Pump User Manual 171

10 Maintenance
Exchanging the Active Inlet Valve (AIV) or its Cartridge

6 Push the cartridge into the new active inlet valve.

6Xi^kZ^caZikVakZXVgig^Y\Z

6Xi^kZ^caZikVakZ

7 Screw the new valve into the pump head. With the 14 mm wrench turn
the nut until it is hand tight.
8 Position the valve so that the solvent inlet tube connection points
towards the front.
9 Using the 14 mm wrench tighten the nut by turning the valve in its
final position (not more than a quarter turn). Do not overtighten the
valve.
10 Reconnect the Active Inlet Valve cable to the connector in the Z- panel
and the inlet tube to the valve.
11 Reinstall the front cover.

After an exchange of the valve it may be required to pump several mL of the solvent used in
NOTE the current application before the flow stabilizes at a pressure ripple as low as it used to be
when the system was still working properly.

172 1260 Infinity Binary Pump User Manual

 Maintenance 10
Exchanging the Outlet Valve

Exchanging the Outlet Valve

When if leaking internally

Tools required Description

Wrench, 1/4 - 1/5 inch
Wrench 1/4 inch
Wrench, 14 mm

Parts required p/n Description

G1312-60067 Outlet valve 1220/1260

Preparations Switch off the pump at the main power switch

1 Using a ¼ inch wrench disconnect the absorber capillary from the

outlet valve.
2 Unscrew the valve with the 14 mm wrench and remove it from the
pump body.
3 Do not disassemble the outlet valve, as this can damage the valve.

4 Reinstall the outlet valve and tighten it using a torque wrench (12 Nm).

1260 Infinity Binary Pump User Manual 173

10 Maintenance
Exchanging the Outlet Valve

5 Reconnect the capillary.

Absorber capillary

Outlet valve

174 1260 Infinity Binary Pump User Manual

 Maintenance 10
Installation of the Solvent Selection Valve Upgrade Kit

Installation of the Solvent Selection Valve Upgrade Kit

A solvent selection valve allows you to choose between four different

solvents that can be used with a binary pump. The valve switches between
two solvents A1 and A2 for channel A of the left pump head and two
solvents B1 and B2 for channel B of the right pump head.

When Applicable modules: This kit is compatible to the 1260 Infinity Binary Pumps G1312B and G1312C
and to the 1260 Infinity Binary Pump Clinical ed. K1312B.

Tools required Description

Screwdriver Pozidriv #1

Parts required p/n Description

G1381-60000 Solvent Selection Valve Upgrade Kit

Preparations If required, remove solvent tubes from the inlet valves.

The figures below show a Binary Pump G1312B. The kit can be used similarly for the Binary
NOTE Pump G1312C and for the Binary Pump Clinical ed. K1312B.

1260 Infinity Binary Pump User Manual 175

10 Maintenance
Installation of the Solvent Selection Valve Upgrade Kit

1 Remove the front cover sheet by removing both screws. 2 Plug in the connector of the solvent selection valve

Connector

SSV cable
Cover sheet

Screws

Solvent
selection valve

3 Install the solvent selection assembly by fixing both 4 Connect the outlet for solvent A (upper row) to the inlet
indicated screws. valve of the left pump head. Connect the outlet for
solvent B (lower row) to the inlet valve of the right pump
head. Put the solvent bottles into the solvent cabinet.
Connect the bottle heads of solvents A1 and A2 to the
inlets in the upper row, see labels on valve assembly.
Connect the bottle heads of solvents B1 and B2 to the
inlets in the lower row, see labels on valve assembly.

To solvent
bottles

Inlets

Outlets

For controlling the solvent selection valve, please refer to

the online help or user manual of your control software.

176 1260 Infinity Binary Pump User Manual

 Maintenance 10
Exchanging the Solvent Selection Valve

Exchanging the Solvent Selection Valve

When If leaking internally (crossflow between the ports), or if one of the channels is blocked

Tools required p/n Description

8710-0899 Screwdriver, Pozidriv #1

Parts required p/n Description

G1381-60000 Solvent Selection Valve Upgrade Kit

Preparations Switch off the pump at the main power switch

1 Lift solvent bottles out of the solvent cabinet and place 2 Disconnect all tubings from the solvent selection valve.
them on the table. Disconnect the solvent tubes from the
solvent selection valve and empty the tubes into the
bottles. Place the bottles back into the solvent cabinet.

1260 Infinity Binary Pump User Manual 177

10 Maintenance
Exchanging the Solvent Selection Valve

3 Using a Pozidriv screwdriver #1 loosen the holding 4 Carefully pull the valve holder out and disconnect the
screws of the valve holder. valve cable at the connector.

5 Exchange the defective solvent selection valve. 6 Tighten the screws of the valve holder.

178 1260 Infinity Binary Pump User Manual

 Maintenance 10
Exchanging the Solvent Selection Valve

7 Reconnect all tubings to the solvent selection valve.

After an exchange of the valve it may be required to pump several mL of solvent before the
NOTE flow stabilizes at a pressure ripple as low as it used to be when the system was still
working properly.

1260 Infinity Binary Pump User Manual 179

10 Maintenance
Exchanging the Optional Interface Board

Exchanging the Optional Interface Board

When Board defective

Parts required # Description

1 BCD (Interface) board

Preparations • Switch OFF the module at the main power switch.

• Unplug the module from main power.

Electronic boards and components are sensitive to electrostatic discharge (ESD).

CAUTION
ESD can damage electronic boards and components.
➔ In order to prevent damage always use an ESD protection when handling electronic
boards and components.

1 Disconnect cables from the interface board connectors.

2 Loosen the screws. Slide out the interface board from the module.

BCD (interface) board

Figure 24 Exchanging the Interface Board

3 Install the new interface board. Secure the screws.

4 Reconnect the cables to the board connector

180 1260 Infinity Binary Pump User Manual

 Maintenance 10
Replacing Module Firmware

Replacing Module Firmware

When The installation of newer firmware might be necessary

• if a newer version solves problems of older versions or
• to keep all systems on the same (validated) revision.
The installation of older firmware might be necessary
• to keep all systems on the same (validated) revision or
• if a new module with newer firmware is added to a system or
• if third party control software requires a special version.

Tools required Description

LAN/RS-232 Firmware Update Tool
OR Agilent Lab Advisor software
OR Instant Pilot G4208A
(only if supported by module)

Parts required # Description

1 Firmware, tools and documentation from Agilent web site

Preparations Read update documentation provided with the Firmware Update Tool.

To upgrade/downgrade the module’s firmware carry out the following

steps:
1 Download the required module firmware, the latest LAN/RS- 232 FW
Update Tool and the documentation from the Agilent web.
• http://www.chem.agilent.com/_layouts/agilent/downloadFirmware.aspx?whid=69761
2 For loading the firmware into the module follow the instructions in the
documentation.

1260 Infinity Binary Pump User Manual 181

10 Maintenance
Replacing Module Firmware

Module Specific Information

Table 13 Module Specific Information (G1312B)

G1312B Binary pump

NOTE
The module G1312B exists both as part of the 1200 Series and 1260 Infinity product lines.
These modules use different solvent selection valves and main boards.
Latest firmware revisions in each set automatically recognize the main board type and
can be used without special handling.
• A.06.06 [020] These revisions recognize the board type and shall be used.
• A.06.10 [020] A.06.06 is the minimum version, which is compatible to 1260
• A.06.34 [001] Infinity Binary Pumps G1312B.
• A.06.53 [002] Older G1312B firmware is only compatible to 1200 Series Binary
Pumps SL G1312B.
All listed firmware revisions allow emulation of G1312A pumps.
Please consult the Firmware Update Guide for 1200 Infinity
Systems for details.

182 1260 Infinity Binary Pump User Manual

 1260 Infinity Binary Pump User Manual

11
Parts and Materials for Maintenance
Hydraulic Path with Solvent Selection Valve 184
Hydraulic Path without Solvent Selection Valve 186
Pump Head Assembly Without Seal Wash 188
Pump Head Assembly with Seal Wash Option 190
Outlet Valve 192
Purge Valve Assembly 193
Active Inlet Valve Assembly 194
HPLC Starter Kit G4201-68707 195
HPLC Starter Kit G4202-68707 195
HPLC System Tool Kit 196
Bottle Head Assembly 197
Solvent Cabinet 198

This chapter lists all parts and tools that are required for maintenance.

Agilent Technologies 183

11 Parts and Materials for Maintenance
Hydraulic Path with Solvent Selection Valve

Hydraulic Path with Solvent Selection Valve

Item p/n Description

1 G1322-67300 Kit of 4 solvent tubes including labels

for connection degasser to SSV

2 G1312-60068 1260 Infinity Solvent selection valve (includes holder)

5041-8365 Blank plug

for unused SSV channels

3 G1312-60003 Connecting tube

1260 Infinity Solvent selection valve to active inlet valve

4 G1312-60025 Active inlet valve body, without cartridge

5 G1312-60045 Pump head assembly with seal wash

6 G1312-60067 Outlet valve 1220/1260

7 G1312-87300 Absorber capillary

8 G1312-67302 Capillary, channel A and B pump head outlet to mixing chamber

(included)

9 G1312-87301 Restriction capillary (mixing capillary to pressure sensor)

11 G1312-87305 Capillary SSL, 0.17 x 150 mm (pressure sensor to damper)

13 G1312-87330 Mixer

14 G1312-87306 Capillary SSL, 0.17 x 105 mm (connections to solvent mixer)

G1312-04100 Bracket for solvent mixer

15 G1312-60061 Purge valve 1260

5042-8507 Peristaltic pump cartridge, silicone tubing

5065-9978 Tubing, 1 mm i.d., 3 mm o.d., silicone, 5 m, for seal wash option

16 G1312-87303 Capillary ST 0.17 mm x 400 mm S/S

G1312-87304 Capillary ST 0.17 mm x 700 mm S/S

17 5062-2461 Waste tube, 5 m (reorder pack)

184 1260 Infinity Binary Pump User Manual

 Parts and Materials for Maintenance 11
Hydraulic Path with Solvent Selection Valve

&& &) . & &' &( -

&%

&* +

,
)

&,

&+ ' (

Figure 25 Hydraulic Path with Solvent Selection Valve

1260 Infinity Binary Pump User Manual 185

11 Parts and Materials for Maintenance
Hydraulic Path without Solvent Selection Valve

Hydraulic Path without Solvent Selection Valve

Item p/n Description

1 G1322-67300 Kit of 4 solvent tubes including labels

for connection degasser to SSV

2 0100-1847 PEEK adapter 1/4-28 to 10-32

(Adapter AIV to solvent inlet tubes)

3 G1312-60025 Active inlet valve body, without cartridge

4 G1312-60056 Pump Head 1200 SL without Seal Wash

5 G1312-60067 Outlet valve 1220/1260

6 G1312-87300 Absorber capillary

7 G1312-67302 Capillary, channel A and B pump head outlet to mixing chamber

(included)

8 G1312-87301 Restriction capillary (mixing capillary to pressure sensor)

10 G1312-87305 Capillary SSL, 0.17 x 150 mm (pressure sensor to damper)

12 G1312-87330 Mixer

13 G1312-87306 Capillary SSL, 0.17 x 105 mm (connections to solvent mixer)

G1312-04100 Bracket for solvent mixer

14 G1312-60061 Purge valve 1260

15 G1312-87303 Capillary ST 0.17 mm x 400 mm S/S

G1312-87304 Capillary ST 0.17 mm x 700 mm S/S

16 5062-2461 Waste tube, 5 m (reorder pack)

17 5042-8507 Peristaltic pump cartridge, silicone tubing

18 5065-9978 Tubing, 1 mm i.d., 3 mm o.d., silicone, 5 m, for seal wash option

186 1260 Infinity Binary Pump User Manual

 Parts and Materials for Maintenance 11
Hydraulic Path without Solvent Selection Valve

&% &( &' & - &-

&,
.

&) *

) ,

'
+
(

&+ &* &&

Figure 26 Hydraulic Path without Solvent Selection Valve, with Active Seal Wash

1260 Infinity Binary Pump User Manual 187

11 Parts and Materials for Maintenance
Pump Head Assembly Without Seal Wash

Pump Head Assembly Without Seal Wash

Item p/n Description

G1312-60056 Pump Head 1200 SL without Seal Wash

1 5063-6586 Sapphire piston

2 G1311-60002 Piston housing

3 5067-1560 Support Ring SL, no seal wash

4 5062-2484 Gasket, seal wash (pack of 6)

5 5042-8952 Seal holder

6 G1312-87300 Absorber capillary

7 5063-6589 Piston seal PTFE, carbon filled, black (pack of 2), default

8 G1311-25200 Pump chamber housing

9 0515-0175 Mounting screw for manual purge valve holder, M4, 20 mm long

10 G1312-23200 Holder for manual purge valve

11 G1312-60061 Purge valve 1260

12 G1312-60067 Outlet valve 1220/1260

13 5042-1303 Lock screw

14a G1312-60025 Active inlet valve body, without cartridge

14b G1312-60020 Cartridge for active inlet valve 600 bar

15 G1312-23201 Adapter

16 0515-2118 Pump head screw (M5, 60 mm)

188 1260 Infinity Binary Pump User Manual

 Parts and Materials for Maintenance 11
Pump Head Assembly Without Seal Wash

&%

&& &

'

&' (
)
&( *

+
,
&*
&+ -

&)V!W

Figure 27 Pump Head Assembly Without Seal Wash

1260 Infinity Binary Pump User Manual 189

11 Parts and Materials for Maintenance
Pump Head Assembly with Seal Wash Option

Pump Head Assembly with Seal Wash Option

Item p/n Description

G1312-60045 Pump head assembly with seal wash

1 5042-8507 Peristaltic pump cartridge, silicone tubing

5065-9978 Tubing, 1 mm i.d., 3 mm o.d., silicone, 5 m, for seal wash option

2 5063-6586 Sapphire piston

3 G1311-60002 Piston housing

4 01018-60027 Support ring seal wash

5 0905-1175 Wash seal (PTFE)

6 5062-2484 Gasket, seal wash (pack of 6)

7 5042-8952 Seal holder

8 G1312-87300 Absorber capillary

9 5063-6589 Piston seal PTFE, carbon filled, black (pack of 2), default

10 0515-0175 Mounting screw for manual purge valve holder, M4, 20 mm long

11 G1312-23200 Holder for manual purge valve

12 G1312-60061 Purge valve 1260

13 G1312-60067 Outlet valve 1220/1260

14 5042-1303 Lock screw

15 G1311-25200 Pump chamber housing

16a G1312-60025 Active inlet valve body, without cartridge

16b G1312-60020 Cartridge for active inlet valve 600 bar

17 G1312-23201 Adapter

18 0515-2118 Pump head screw (M5, 60 mm)

190 1260 Infinity Binary Pump User Manual

 Parts and Materials for Maintenance 11
Pump Head Assembly with Seal Wash Option

10

11
2
12
3
4
13 5
6
14 7

8
9
17
18 15

16 a, b

Figure 28 Pump head assembly with seal wash option

1260 Infinity Binary Pump User Manual 191

11 Parts and Materials for Maintenance
Outlet Valve

Outlet Valve

p/n Description

G1312-60067 Outlet valve 1220/1260

Figure 29 Outlet Valve

192 1260 Infinity Binary Pump User Manual

 Parts and Materials for Maintenance 11
Purge Valve Assembly

Purge Valve Assembly

Item p/n Description

1 G1312-60061 Purge valve 1260

2 01018-22707 PTFE frits (pack of 5)

3 5067-4728 Seal cap

3
Figure 30 Purge Valve Assembly

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11 Parts and Materials for Maintenance
Active Inlet Valve Assembly

Active Inlet Valve Assembly

Item p/n Description

1 G1312-60025 Active inlet valve body, without cartridge

2 G1312-60020 Cartridge for active inlet valve 600 bar

Figure 31 Active Inlet Valve Assembly

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 Parts and Materials for Maintenance 11
HPLC Starter Kit G4201-68707

HPLC Starter Kit G4201-68707

HPLC Starter Kit incl. 0.17 mm i.d. cap (G4201- 68707)

p/n Description

9301-1420 (3x) Solvent bottle, transparent

9301-1450 Solvent bottle, amber

01018-22707 PTFE frits (pack of 5)

5182-0716 Screw Cap Vial, 2 mL, amber glass, write-on spot, 100/pk

5182-0717 Blue screw caps 100/pk

5063-6507 (2x) Chip, Column I.D. Assy

5041-2168 (2x) Solvent inlet filter, 20 µm pore size

5065-9939 Capillary/Fitting Starter Kit 0.17 mm id

HPLC Starter Kit G4202-68707

HPLC Starter Kit incl. 0.12 mm i.d. cap (G4202- 68707)

p/n Description

9301-1420 (3x) Solvent bottle, transparent

9301-1450 Solvent bottle, amber

01018-22707 PTFE frits (pack of 5)

5182-0716 Screw Cap Vial, 2 mL, amber glass, write-on spot, 100/pk

5182-0717 Blue screw caps 100/pk

5063-6507 (2x) Chip, Column I.D. Assy

5041-2168 (2x) Solvent inlet filter, 20 µm pore size

G1316-80003 Heater long-down (0.12 mm i.d., 1.6 µL internal volume)

5065-9937 Capillary/Fitting Starter Kit 0.12 mm id

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11 Parts and Materials for Maintenance
HPLC System Tool Kit

HPLC System Tool Kit

The HPLC System Tool Kit (G4203- 68708) contains some accessories and
tools needed for installation and repair of the module.

p/n Description

0100-1681 Adapter syringe/seal wash tube

0100-1710 Mounting Tool for Tubing Connections

01018-23702 Insert tool

5023-0240 Hex driver, ¼", slitted

8710-0060 Hex-key wrench, 9/64 inch

8710-0510 (2x) Wrench open 1/4 — 5/16 inch

8710-0641 Hex key set 1 – 5 mm

8710-0899 Pozidriv screwdriver

8710-1534 Wrench, 4 mm both ends, open end

8710-1924 Wrench open 14 mm

8710-2392 Hex key 4 mm15 cm long T-handle

8710-2393 Hex key 1.5 mm, straight handle 10 cm

8710-2394 Hex key 9/64 inch 15 cm long T-handle

8710-2409 Wrench open end, 5/16 – 3/8 inch

8710-2411 Hex key 3 mm12 cm long

8710-2412 Hex key 2.5 mm, 15 cm long, straight handle

8710-2438 Hex key 2.0 mm

8710-2509 Screwdriver Torx TX8

8710-2594 Double open end wrench 4 mm

9301-0411 Syringe, Plastic

9301-1337 Adapter syringe/solvent tube with fitting

196 1260 Infinity Binary Pump User Manual

 Parts and Materials for Maintenance 11
Bottle Head Assembly

Bottle Head Assembly

Item p/n Description

1 9301-1450 Solvent bottle, amber

2 9301-1420 Solvent bottle, transparent

3 G1311-60003 Bottle-head assembly

4 5063-6598 Ferrules with lock ring (10/Pk)

5 5063-6599 Tube screw (10/Pk)

6 5062-2483 Solvent tubing, 5 m

7 5062-8517 Inlet filter adapter (4/Pk)

8 5041-2168 Solvent inlet filter, 20 µm pore size

)
( *

&$'

,
-

Figure 32 Bottle-Head Assembly Parts

1260 Infinity Binary Pump User Manual 197

11 Parts and Materials for Maintenance
Solvent Cabinet

Solvent Cabinet

'

&

( )
Figure 33 Solvent Cabinet Parts (1)

*$+

Figure 34 Solvent Cabinet Parts (2)

198 1260 Infinity Binary Pump User Manual

 Parts and Materials for Maintenance 11
Solvent Cabinet

Item p/n Description

1 5065-9981 Solvent cabinet 1200 Infinity, including all plastic parts

2 5043-0207 Name plate 1260

3 5065-9954 Front panel, solvent cabinet

4 5042-8907 Leak panel

5 9301-1450 Solvent bottle, amber

6 9301-1420 Solvent bottle, transparent

7 G1311-60003 Bottle-head assembly

1260 Infinity Binary Pump User Manual 199

11 Parts and Materials for Maintenance
Solvent Cabinet

200 1260 Infinity Binary Pump User Manual

 1260 Infinity Binary Pump User Manual

12
Identifying Cables
Overview 202
Analog cables 204
Remote Cables 206
BCD Cables 209
CAN/LAN Cables 211
External Contact Cables 212
RS-232 Cable Kit 213
Agilent 1200 Module to Printer 214

This chapter provides information on cables used with the Agilent 1200 Infinity
Series modules.

Agilent Technologies 201

12 Identifying Cables
Overview

Overview

Never use cables other than the ones supplied by Agilent Technologies to ensure proper
NOTE functionality and compliance with safety or EMC regulations.

Analog cables

p/n Description

35900-60750 Agilent module to 3394/6 integrators

35900-60750 Agilent 35900A A/D converter

01046-60105 Analog cable (BNC to general purpose, spade lugs)

Remote cables

p/n Description

03394-60600 Agilent module to 3396A Series I integrators

3396 Series II / 3395A integrator, see details in section “Remote

Cables” on page 206

03396-61010 Agilent module to 3396 Series III / 3395B integrators

5061-3378 Remote Cable

01046-60201 Agilent module to general purpose

BCD cables

p/n Description

03396-60560 Agilent module to 3396 integrators

G1351-81600 Agilent module to general purpose

202 1260 Infinity Binary Pump User Manual

 Identifying Cables 12
Overview

CAN cables

p/n Description

5181-1516 CAN cable, Agilent module to module, 0.5 m

5181-1519 CAN cable, Agilent module to module, 1 m

LAN cables

p/n Description

5023-0203 Cross-over network cable, shielded, 3 m (for point to point connection)

5023-0202 Twisted pair network cable, shielded, 7 m (for point to point connection)

External Contact Cable

p/n Description

G1103-61611 External contact cable - Agilent module interface board to general

purposes

RS-232 cables

p/n Description

G1530-60600 RS-232 cable, 2 m

RS232-61601 RS-232 cable, 2.5 m

Instrument to PC, 9-to-9 pin (female). This cable has special pin-out,
and is not compatible with connecting printers and plotters. It's also
called "Null Modem Cable" with full handshaking where the wiring is
made between pins 1-1, 2-3, 3-2, 4-6, 5-5, 6-4, 7-8, 8-7, 9-9.

5181-1561 RS-232 cable, 8 m

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12 Identifying Cables
Analog cables

Analog cables

One end of these cables provides a BNC connector to be connected to

Agilent modules. The other end depends on the instrument to which
connection is being made.

Agilent Module to 3394/6 Integrators

p/n 35900-60750 Pin 3394/6 Pin Agilent Signal Name

module

1 Not connected

2 Shield Analog -

3 Center Analog +

Agilent Module to BNC Connector

p/n 8120-1840 Pin BNC Pin Agilent Signal Name

module

Shield Shield Analog -

Center Center Analog +

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 Identifying Cables 12
Analog cables

Agilent Module to General Purpose

p/n 01046-60105 Pin Pin Agilent Signal Name

module

1 Not connected

2 Black Analog -

3 Red Analog +

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12 Identifying Cables
Remote Cables

Remote Cables

One end of these cables provides a Agilent Technologies APG (Analytical

Products Group) remote connector to be connected to Agilent modules.
The other end depends on the instrument to be connected to.

Agilent Module to 3396A Integrators

p/n 03394-60600 Pin 3396A Pin Agilent Signal Name Active

module (TTL)

9 1 - White Digital ground

NC 2 - Brown Prepare run Low

3 3 - Gray Start Low

NC 4 - Blue Shut down Low

NC 5 - Pink Not
connected

NC 6 - Yellow Power on High

5,14 7 - Red Ready High

1 8 - Green Stop Low

NC 9 - Black Start request Low

13, 15 Not
connected

Agilent Module to 3396 Series II / 3395A Integrators

Use the cable Agilent module to 3396A Series I integrators (03394- 60600)
and cut pin #5 on the integrator side. Otherwise the integrator prints
START; not ready.

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 Identifying Cables 12
Remote Cables

Agilent Module to 3396 Series III / 3395B Integrators

p/n 03396-61010 Pin 33XX Pin Agilent Signal Name Active

module (TTL)

9 1 - White Digital ground

NC 2 - Brown Prepare run Low

3 3 - Gray Start Low

NC 4 - Blue Shut down Low

NC 5 - Pink Not connected

NC 6 - Yellow Power on High

14 7 - Red Ready High

4 8 - Green Stop Low

NC 9 - Black Start request Low

13, 15 Not connected

Agilent Module to Agilent 35900 A/D Converters

p/n 5061-3378 Pin 35900 A/D Pin Agilent Signal Name Active
module (TTL)

1 - White 1 - White Digital ground

2 - Brown 2 - Brown Prepare run Low

3 - Gray 3 - Gray Start Low

4 - Blue 4 - Blue Shut down Low

5 - Pink 5 - Pink Not connected

6 - Yellow 6 - Yellow Power on High

7 - Red 7 - Red Ready High

8 - Green 8 - Green Stop Low

9 - Black 9 - Black Start request Low

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12 Identifying Cables
Remote Cables

Agilent Module to General Purpose

p/n 01046-60201 Wire Color Pin Agilent Signal Name Active

module (TTL)

White 1 Digital ground

Brown 2 Prepare run Low

Gray 3 Start Low

Blue 4 Shut down Low

Pink 5 Not
connected

Yellow 6 Power on High

Red 7 Ready High

Green 8 Stop Low

Black 9 Start request Low

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 Identifying Cables 12
BCD Cables

BCD Cables

One end of these cables provides a 15- pin BCD connector to be connected
to the Agilent modules. The other end depends on the instrument to be
connected to

Agilent Module to General Purpose

p/n G1351-81600 Wire Color Pin Agilent Signal Name BCD Digit
module

Green 1 BCD 5 20

Violet 2 BCD 7 80

Blue 3 BCD 6 40

Yellow 4 BCD 4 10

Black 5 BCD 0 1

Orange 6 BCD 3 8

Red 7 BCD 2 4
Brown 8 BCD 1 2

Gray 9 Digital ground Gray

Gray/pink 10 BCD 11 800

Red/blue 11 BCD 10 400

White/green 12 BCD 9 200

Brown/green 13 BCD 8 100

not connected 14

not connected 15 +5V Low

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12 Identifying Cables
BCD Cables

Agilent Module to 3396 Integrators

p/n 03396-60560 Pin 3396 Pin Agilent Signal Name BCD Digit
module

1 1 BCD 5 20

2 2 BCD 7 80

3 3 BCD 6 40

4 4 BCD 4 10

5 5 BCD0 1

6 6 BCD 3 8

7 7 BCD 2 4

8 8 BCD 1 2

9 9 Digital ground

NC 15 +5V Low

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 Identifying Cables 12
CAN/LAN Cables

CAN/LAN Cables

Both ends of this cable provide a modular plug to be connected to

Agilent modules CAN or LAN connectors.
CAN Cables

p/n Description

5181-1516 CAN cable, Agilent module to module, 0.5 m

5181-1519 CAN cable, Agilent module to module, 1 m

LAN Cables

p/n Description

5023-0203 Cross-over network cable, shielded, 3 m (for point to point connection)

5023-0202 Twisted pair network cable, shielded, 7 m (for point to point connection)

1260 Infinity Binary Pump User Manual 211

12 Identifying Cables
External Contact Cables

External Contact Cables

5 1
10 6
15 11

One end of this cable provides a 15- pin plug to be connected to

Agilent modules interface board. The other end is for general purpose.

Agilent Module Interface Board to general purposes

p/n G1103-61611 Color Pin Agilent Signal Name

module

White 1 EXT 1

Brown 2 EXT 1

Green 3 EXT 2

Yellow 4 EXT 2

Grey 5 EXT 3

Pink 6 EXT 3

Blue 7 EXT 4
Red 8 EXT 4

Black 9 Not connected

Violet 10 Not connected

Grey/pink 11 Not connected

Red/blue 12 Not connected

White/green 13 Not connected

Brown/green 14 Not connected

White/yellow 15 Not connected

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 Identifying Cables 12
RS-232 Cable Kit

RS-232 Cable Kit

p/n Description

G1530-60600 RS-232 cable, 2 m

RS232-61601 RS-232 cable, 2.5 m

Instrument to PC, 9-to-9 pin (female). This cable has special pin-out, and
is not compatible with connecting printers and plotters. It's also called
"Null Modem Cable" with full handshaking where the wiring is made
between pins 1-1, 2-3, 3-2, 4-6, 5-5, 6-4, 7-8, 8-7, 9-9.

5181-1561 RS-232 cable, 8 m

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12 Identifying Cables
Agilent 1200 Module to Printer

Agilent 1200 Module to Printer

p/n Description

5181-1529 Cable Printer Serial & Parallel, is a SUB-D 9 pin female vs. Centronics
connector on the other end (NOT FOR FW UPDATE). For use with
G1323 Control Module.

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 1260 Infinity Binary Pump User Manual

13
Hardware Information
Firmware Description 216
Electrical Connections 219
Rear view of the module 220
Interfaces 221
Overview Interfaces 224
Setting the 8-bit Configuration Switch (without On-board) LAN 228
Communication Settings for RS-232C 229
Special Settings 231
Optional Interface Boards 232
The Solvent Selection Valve (SSV) 236
Early Maintenance Feedback 237
Instrument Layout 238

This chapter provides detailed technical information about your binary pump.

Agilent Technologies 215

13 Hardware Information
Firmware Description

Firmware Description

The firmware of the instrument consists of two independent sections:

• a non- instrument specific section, called resident system
• an instrument specific section, called main system

Resident System
This resident section of the firmware is identical for all Agilent
1100/1200/1220/1260/1290 series modules. Its properties are:
• the complete communication capabilities (CAN, LAN and RS- 232C)
• memory management
• ability to update the firmware of the 'main system'

Main System
Its properties are:
• the complete communication capabilities (CAN, LAN and RS- 232C)
• memory management
• ability to update the firmware of the 'resident system'
In addition the main system comprises the instrument functions that are
divided into common functions like
• run synchronization through APG remote,
• error handling,
• diagnostic functions,
• or module specific functions like
• internal events such as lamp control, filter movements,
• raw data collection and conversion to absorbance.

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 Hardware Information 13
Firmware Description

Firmware Updates
Firmware updates can be done using your user interface:
• PC and Firmware Update Tool with local files on the hard disk
• Instant Pilot (G4208A) with files from a USB Flash Disk
• Agilent Lab Advisor software B.01.03 and above
The file naming conventions are:
PPPP_RVVV_XXX.dlb, where
PPPP is the product number, for example, 1315AB for the G1315A/B DAD,
R the firmware revision, for example, A for G1315B or B for the G1315C
DAD,
VVV is the revision number, for example 102 is revision 1.02,
XXX is the build number of the firmware.
For instructions on firmware updates refer to section Replacing Firmware
in chapter "Maintenance" or use the documentation provided with the
Firmware Update Tools.

Update of main system can be done in the resident system only. Update of the resident
NOTE system can be done in the main system only.
Main and resident firmware must be from the same set.

BV^c;LjeYViZ
GZh^YZciHnhiZb BV^cHnhiZb

GZh^YZci;LJeYViZ

Figure 35 Firmware Update Mechanism

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13 Hardware Information
Firmware Description

Some modules are limited in downgrading due to their main board version or their initial
NOTE firmware revision. For example, a G1315C DAD SL cannot be downgraded below firmware
revision B.01.02 or to a A.xx.xx.
Some modules can be re-branded (e.g. G1314C to G1314B) to allow operation in specific
control software environments. In this case the feature set of the target type are use and
the feature set of the original are lost. After re-branding (e.g. from G1314B to G1314C), the
original feature set is available again.
All these specific informations are described in the documentation provided with the
firmware update tools.

The firmware update tools, firmware and documentation are available

from the Agilent web.
• http://www.chem.agilent.com/_layouts/agilent/downloadFirmware.aspx?whid=69761

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 Hardware Information 13
Electrical Connections

Electrical Connections

• The CAN bus is a serial bus with high speed data transfer. The two
connectors for the CAN bus are used for internal module data transfer
and synchronization.
• One analog output provides signals for integrators or data handling
systems.
• The interface board slot is used for external contacts and BCD bottle
number output or LAN connections.
• The REMOTE connector may be used in combination with other
analytical instruments from Agilent Technologies if you want to use
features such as start, stop, common shut down, prepare, and so on.
• With the appropriate software, the RS- 232C connector may be used to
control the module from a computer through a RS- 232C connection.
This connector is activated and can be configured with the
configuration switch.
• The power input socket accepts a line voltage of 100 – 240 VAC ± 10 %
with a line frequency of 50 or 60 Hz. Maximum power consumption
varies by module. There is no voltage selector on your module because
the power supply has wide- ranging capability. There are no externally
accessible fuses, because automatic electronic fuses are implemented in
the power supply.

Never use cables other than the ones supplied by Agilent Technologies to ensure proper
NOTE functionality and compliance with safety or EMC regulations.

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13 Hardware Information
Electrical Connections

Rear view of the module

Configuration switch

Slot for interface board

RS232

Remote

Analog output

CAN-Bus
HP-IB

Power plug

Figure 36 Electrical connections to the binary pump

220 1260 Infinity Binary Pump User Manual

 Hardware Information 13
Interfaces

Interfaces

The Agilent 1200 Infinity Series modules provide the following interfaces:

Table 14 Agilent 1200 Infinity Series Interfaces

Module CAN LAN/BCD LAN RS-232 Analog APG Special

(optional) (on-board) Remote

Pumps

G1310B Iso Pump 2 Yes No Yes 1 Yes

G1311B Quat Pump
G1311C Quat Pump VL
G1312B Bin Pump
K1312B Bin Pump Clinical Ed.
G1312C Bin Pump VL
1376A Cap Pump
G2226A Nano Pump
G5611A Bio-inert Quat Pump

G4220A/B Bin Pump 2 No Yes Yes No Yes CAN-DC- OUT for CAN
G4204A Quat Pump slaves

G1361A Prep Pump 2 Yes No Yes No Yes CAN-DC- OUT for CAN
slaves

Samplers

G1329B ALS 2 Yes No Yes No Yes THERMOSTAT for

G2260A Prep ALS G1330B/K1330B

G1364B FC-PS 2 Yes No Yes No Yes THERMOSTAT for

G1364C FC-AS G1330B/K1330B
G1364D FC-μS CAN-DC- OUT for CAN
G1367E HiP ALS slaves
K1367E HiP ALS Clinical Ed.
G1377A HiP micro ALS
G2258A DL ALS
G5664A Bio-inert FC-AS
G5667A Bio-inert
Autosampler

G4226A ALS 2 Yes No Yes No Yes

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13 Hardware Information
Interfaces

Table 14 Agilent 1200 Infinity Series Interfaces

Module CAN LAN/BCD LAN RS-232 Analog APG Special

(optional) (on-board) Remote
Detectors

G1314B VWD VL 2 Yes No Yes 1 Yes

G1314C VWD VL+

G1314E/F VWD 2 No Yes Yes 1 Yes

K1314F Clinical Ed.

G4212A/B DAD 2 No Yes Yes 1 Yes

K4212B DAD Clinical Ed.

G1315C DAD VL+ 2 No Yes Yes 2 Yes

G1365C MWD
G1315D DAD VL
G1365D MWD VL
G1321B FLD 2 Yes No Yes 2 Yes
K1321B FLD Clinical Ed.
G1321C FLD

G1362A RID 2 Yes No Yes 1 Yes

G4280A ELSD No No No Yes Yes Yes EXT Contact

AUTOZERO

Others

G1170A Valve Drive 2 No No No No No 1

G1316A/C TCC 2 No No Yes No Yes

K1316C TCC Clinical Ed.

G1322A DEG No No No No No Yes AUX

K1322A DEG Clinical Ed.

G1379B DEG No No No Yes No Yes

G4225A DEG No No No Yes No Yes

K4225A DEG Clinical Ed.

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 Hardware Information 13
Interfaces

Table 14 Agilent 1200 Infinity Series Interfaces

Module CAN LAN/BCD LAN RS-232 Analog APG Special

(optional) (on-board) Remote
G4227A Flex Cube 2 No No No No No CAN-DC- OUT for CAN
slaves
1

G4240A CHIP CUBE 2 Yes No Yes No Yes CAN-DC- OUT for CAN
slaves
THERMOSTAT for
G1330A/B (NOT
USED), K1330B
1
Requires a HOST module with on-board LAN (e.g. G4212A or G4220A with minimum firmware B.06.40 or C.06.40) or with ad-
ditional G1369C LAN Card

The detector (DAD/MWD/FLD/VWD/RID) is the preferred access point for control via
NOTE LAN. The inter-module communication is done via CAN.

• CAN connectors as interface to other modules

• LAN connector as interface to the control software
• RS- 232C as interface to a computer
• REMOTE connector as interface to other Agilent products
• Analog output connector(s) for signal output

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13 Hardware Information
Interfaces

Overview Interfaces
CAN
The CAN is inter- module communication interface. It is a 2- wire serial
bus system supporting high speed data communication and real- time
requirement.

LAN
The modules have either an interface slot for an LAN card (e.g. Agilent
G1369B/C LAN Interface) or they have an on- board LAN interface (e.g.
detectors G1315C/D DAD and G1365C/D MWD). This interface allows the
control of the module/system via a PC with the appropriate control
software. Some modules have neither on- board LAN nor an interface slot
for a LAN card (e.g. G1170A Valve Drive or G4227A Flex Cube). These are
hosted modules and require a Host module with firmware B.06.40 or later
or with additional G1369C LAN Card.

If an Agilent detector (DAD/MWD/FLD/VWD/RID) is in the system, the LAN should be

NOTE connected to the DAD/MWD/FLD/VWD/RID (due to higher data load). If no Agilent
detector is part of the system, the LAN interface should be installed in the pump or
autosampler.

RS-232C (Serial)
The RS- 232C connector is used to control the module from a computer
through RS- 232C connection, using the appropriate software. This
connector can be configured with the configuration switch module at the
rear of the module. Refer to Communication Settings for RS- 232C.

There is no configuration possible on main boards with on-board LAN. These are
NOTE pre-configured for
• 19200 baud,
• 8 data bit with no parity and
• one start bit and one stop bit are always used (not selectable).

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 Hardware Information 13
Interfaces

The RS- 232C is designed as DCE (data communication equipment) with a

9- pin male SUB- D type connector. The pins are defined as:

Table 15 RS-232C Connection Table

Pin Direction Function

1 In DCD

2 In RxD

3 Out TxD

4 Out DTR

5 Ground

6 In DSR

7 Out RTS

8 In CTS

9 In RI

>chigjbZci E8

BVaZ ;ZbVaZ ;ZbVaZ BVaZ

Figure 37 RS-232 Cable

Analog Signal Output

The analog signal output can be distributed to a recording device. For
details refer to the description of the module’s main board.

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13 Hardware Information
Interfaces

APG Remote
The APG Remote connector may be used in combination with other
analytical instruments from Agilent Technologies if you want to use
features as common shut down, prepare, and so on.
Remote control allows easy connection between single instruments or
systems to ensure coordinated analysis with simple coupling requirements.
The subminiature D connector is used. The module provides one remote
connector which is inputs/outputs (wired- or technique).
To provide maximum safety within a distributed analysis system, one line
is dedicated to SHUT DOWN the system’s critical parts in case any module
detects a serious problem. To detect whether all participating modules are
switched on or properly powered, one line is defined to summarize the
POWER ON state of all connected modules. Control of analysis is
maintained by signal readiness READY for next analysis, followed by START
of run and optional STOP of run triggered on the respective lines. In
addition PREPARE and START REQUEST may be issued. The signal levels are
defined as:
• standard TTL levels (0 V is logic true, + 5.0 V is false),
• fan- out is 10 ,
• input load is 2.2 kOhm against + 5.0 V, and
• output are open collector type, inputs/outputs (wired- or technique).

All common TTL circuits operate with a 5 V power supply. A TTL signal is defined as "low"
NOTE or L when between 0 V and 0.8 V and "high" or H when between 2.0 V and 5.0 V (with
respect to the ground terminal).

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 Hardware Information 13
Interfaces

Table 16 Remote Signal Distribution

Pin Signal Description

1 DGND Digital ground

2 PREPARE (L) Request to prepare for analysis (for example, calibration, detector
lamp on). Receiver is any module performing pre-analysis activities.

3 START (L) Request to start run / timetable. Receiver is any module

performing run-time controlled activities.

4 SHUT DOWN (L) System has serious problem (for example, leak: stops pump).
Receiver is any module capable to reduce safety risk.

5 Not used

6 POWER ON (H) All modules connected to system are switched on. Receiver is
any module relying on operation of others.

7 READY (H) System is ready for next analysis. Receiver is any sequence
controller.

8 STOP (L) Request to reach system ready state as soon as possible (for
example, stop run, abort or finish and stop injection). Receiver is any
module performing run-time controlled activities.

9 START REQUEST (L) Request to start injection cycle (for example, by start key on any
module). Receiver is the autosampler.

Special Interfaces
There is no special interface for this module.

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13 Hardware Information
Setting the 8-bit Configuration Switch (without On-board) LAN

Setting the 8-bit Configuration Switch (without On-board) LAN

The 8- bit configuration switch is located at the rear of the module.

This module does not have its own on- board LAN interface. It can be
controlled through the LAN interface of another module, and a CAN
connection to that module.

Figure 38 Configuration switch (settings depend on configured mode)

All modules without on- board LAN:

• default should be ALL DIPS DOWN (= best settings)
• Bootp mode for LAN and
• 19200 baud, 8 data bit / 1 stop bit with no parity for RS- 232
• DIP 1 DOWN and DIP 2 UP allows special RS- 232 settings
• for boot/test modes DIPS 1+2 must be UP plus required mode

For normal operation use the default (best) settings.

NOTE

Switch settings provide configuration parameters for serial communication

protocol and instrument specific initialization procedures.

With the introduction of the Agilent 1260 Infinity, all GPIB interfaces have been removed.
NOTE The preferred communication is LAN.

228 1260 Infinity Binary Pump User Manual

 Hardware Information 13
Setting the 8-bit Configuration Switch (without On-board) LAN

The following tables represent the configuration switch settings for the modules without
NOTE on-board LAN only.

Table 17 8-bit Configuration Switch (without on-board LAN)

Mode Select 1 2 3 4 5 6 7 8

RS-232C 0 1 Baudrate Data Parity

Bits

Reserved 1 0 Reserved

TEST/BOOT 1 1 RSVD SYS RSVD RSVD FC

The LAN settings are done on the LAN Interface Card G1369B/C. Refer to the
NOTE documentation provided with the card.

Communication Settings for RS-232C

The communication protocol used in the column compartment supports
only hardware handshake (CTS/RTR).
Switches 1 in down and 2 in up position define that the RS- 232C
parameters will be changed. Once the change has been completed, the
column instrument must be powered up again in order to store the values
in the non- volatile memory.

Table 18 Communication Settings for RS-232C Communication (without on-board LAN)

Mode
1 2 3 4 5 6 7 8
Select

RS-232C 0 1 Baudrate Data Bits Parity

Use the following tables for selecting the setting which you want to use for
RS- 232C communication. The number 0 means that the switch is down
and 1 means that the switch is up.

1260 Infinity Binary Pump User Manual 229

13 Hardware Information
Setting the 8-bit Configuration Switch (without On-board) LAN

Table 19 Baudrate Settings (without on-board LAN)

Switches Baud Rate Switches Baud Rate

3 4 5 3 4 5

0 0 0 9600 1 0 0 9600

0 0 1 1200 1 0 1 14400

0 1 0 2400 1 1 0 19200

0 1 1 4800 1 1 1 38400

Table 20 Data Bit Settings (without on-board LAN)

Switch 6 Data Word Size

0 7 Bit Communication

1 8 Bit Communication

Table 21 Parity Settings (without on-board LAN)

Switches Parity

7 8

0 0 No Parity

0 1 Odd Parity

1 1 Even Parity

One start bit and one stop bit are always used (not selectable).
Per default, the module will turn into 19200 baud, 8 data bit with no
parity.

230 1260 Infinity Binary Pump User Manual

 Hardware Information 13
Setting the 8-bit Configuration Switch (without On-board) LAN

Special Settings
The special settings are required for specific actions (normally in a service case).

Boot-Resident
Firmware update procedures may require this mode in case of firmware
loading errors (main firmware part).
If you use the following switch settings and power the instrument up
again, the instrument firmware stays in the resident mode. It is not
operable as a module. It only uses basic functions of the operating system
for example, for communication. In this mode the main firmware can be
loaded (using update utilities).

Table 22 Boot Resident Settings (without on-board LAN)

Mode Select SW1 SW2 SW3 SW4 SW5 SW6 SW7 SW8

TEST/BOOT 1 1 0 0 1 0 0 0

Forced Cold Start

A forced cold start can be used to bring the module into a defined mode
with default parameter settings.

Loss of data
CAUTION
Forced cold start erases all methods and data stored in the non-volatile memory.
Exceptions are calibration settings, diagnosis and repair log books which will not be
erased.
➔ Save your methods and data before executing a forced cold start.

If you use the following switch settings and power the instrument up
again, a forced cold start has been completed.

Table 23 Forced Cold Start Settings (without on-board LAN)

Mode Select SW1 SW2 SW3 SW4 SW5 SW6 SW7 SW8

TEST/BOOT 1 1 0 0 0 0 0 1

1260 Infinity Binary Pump User Manual 231

13 Hardware Information
Optional Interface Boards

Optional Interface Boards

BCD / External Contact Board

The Agilent 1200 Infinity Series modules have one optional board slot that
allows to add an interface board to the modules. Some modules do not
have this interface slot. Refer to “Interfaces” on page 221 for details.
Optional Interface Boards

p/n Description

G1351-68701 Interface board (BCD) with external contacts and BCD outputs

2110-0004 Fuse for BCD board, 250 mA

The BCD board provides a BCD output for the bottle number of the
Agilent 1200 Series autosampler and four external contacts. The external
contact closure contacts are relay contacts. The maximum settings are:
30 V (AC/DC); 250 mA (fused).

7dVgY
^YZci^[^XVi^dc
G;>[^aiZg

&'
EgdXZhhdg 789 789
^ciZg[VXZ gZ\^hiZg XdccZXidg
G;>[^aiZg

A^cZYg^kZg
'*%b6
:miZgcVa )m :miZgcVa
XdciVXih XdciVXi
XdccZXidg

232 1260 Infinity Binary Pump User Manual

 Hardware Information 13
Optional Interface Boards

There are general purpose cables available to connect the BCD output, see
“BCD Cables” on page 209 and the external outputs, see “External Contact
Cables” on page 212 to external devices.

Table 24 Detailed connector layout (1200)

Pin Signal name BCD digit

1 BCD 5 20

2 BCD 7 80

3 BCD 6 40

4 BCD 4 10

5 BCD 0 1

6 BCD 3 8

7 BCD 2 4

8 BCD 1 2

9 Digital ground

10 BCD 11 800

11 BCD 10 400

12 BCD 9 200

13 BCD 8 100

15 +5V Low

1260 Infinity Binary Pump User Manual 233

13 Hardware Information
Optional Interface Boards

LAN Communication Interface Board

The Agilent modules have one optional board slot that allows to add an
interface board to the modules. Some modules do not have this interface
slot. Refer to “Interfaces” on page 221 for details.

p/n Description

G1369B or Interface board (LAN)

G1369-60002

OR G1369C or Interface board (LAN)

G1369-60012

One board is required per Agilent 1260 Infinity instrument. It is recommended to add the
NOTE LAN board to the detector with highest data rate.

For the configuration of the G1369 LAN Communication Interface card refer to its
NOTE documentation.

234 1260 Infinity Binary Pump User Manual

 Hardware Information 13
Optional Interface Boards

The following cards can be used with the Agilent 1260 Infinity modules.

Table 25 LAN Boards

Type Vendor Supported networks

Interface board (LAN) (G1369B Agilent Technologies Fast Ethernet, Ethernet/802.3,

or G1369-60002) or RJ-45 (10/100Base-TX)
Interface board (LAN) (G1369C recommended for re-ordering
or G1369-60012)

LAN Communication Interface Agilent Technologies Fast Ethernet, Ethernet/802.3,

board (G1369A or RJ-45 (10/100Base-TX)
G1369-60001) (obsolete)

J4106A1 Hewlett Packard Ethernet/802.3, RJ-45

(10Base-T)

J4105A1 Hewlett Packard Token Ring/802.5, DB9, RJ-45

(10Base-T)

J4100A1 Hewlett Packard Fast Ethernet, Ethernet/802.3,

RJ-45 (10/100Base-TX) + BNC
(10Base2)

1
These cards may be no longer orderable. Minimum firmware of these Hewlett Packard JetDirect
cards is A.05.05.

Recommended LAN Cables

p/n Description

5023-0203 Cross-over network cable, shielded, 3 m (for point to point connection)

5023-0202 Twisted pair network cable, shielded, 7 m (for point to point connection)

1260 Infinity Binary Pump User Manual 235

13 Hardware Information
The Solvent Selection Valve (SSV)

The Solvent Selection Valve (SSV)

The solvent selection valve is available optionally and as an upgrade. It

allows using four different solvents for binary pumps. The available
channels are A1/A2 and B1/B2. The valve can switch between positions 1
and 2, i.e. two different solvents are available per channel. It is not
possible to switch for example between A1 and B1 for channel B, as
solvent paths A and B are completely separated. By cascading SSV
channels, for example using inlets A1 and A2 and connecting the outlet of
channel A to inlet B1, it is possible to switch between three (A1/A2/B2)
solvents for one channel (here: B) and using always the same solvent for
channel A.
Technically, the 1260 Infinity SSV uses two electromagnetic valves. If the
current is off, inlets A2 and B2 are connected to the outlet. If the current
is on, the valves switch to A1 and/or B1. This means, if the pump is
switched off physically, solvent connections use inlets A2 and B2. If the
pump is in standby (for example after using the Off button in
ChemStation), the SSV retains its previous state.
Unused channels of an SSV should always be blocked using a plug (Blank
plug (5041- 8365)) in order to avoid leaks or air entering the solvent
channels.

236 1260 Infinity Binary Pump User Manual

 Hardware Information 13
Early Maintenance Feedback

Early Maintenance Feedback

Maintenance requires the exchange of components which are subject to

wear or stress. Ideally, the frequency at which components are exchanged
should be based on the intensity of usage of the module and the analytical
conditions, and not on a predefined time interval. The early maintenance
feedback (EMF) feature monitors the usage of specific components in the
instrument, and provides feedback when the user- selectable limits have
been exceeded. The visual feedback in the user interface provides an
indication that maintenance procedures should be scheduled.

EMF Counters
EMF counters increment with use and can be assigned a maximum limit
which provides visual feedback in the user interface when the limit is
exceeded. Some counters can be reset to zero after the required
maintenance procedure.

Using the EMF Counters

The user- settable EMF limits for the EMF Counters enable the early
maintenance feedback to be adapted to specific user requirements. The
useful maintenance cycle is dependent on the requirements for use.
Therefore, the definition of the maximum limits need to be determined
based on the specific operating conditions of the instrument.

Setting the EMF Limits

The setting of the EMF limits must be optimized over one or two
maintenance cycles. Initially the default EMF limits should be set. When
instrument performance indicates maintenance is necessary, take note of
the values displayed by the EMF counters. Enter these values (or values
slightly less than the displayed values) as EMF limits, and then reset the
EMF counters to zero. The next time the EMF counters exceed the new EMF
limits, the EMF flag will be displayed, providing a reminder that
maintenance needs to be scheduled.

1260 Infinity Binary Pump User Manual 237

13 Hardware Information
Instrument Layout

Instrument Layout

The industrial design of the module incorporates several innovative

features. It uses Agilent’s E- PAC concept for the packaging of electronics
and mechanical assemblies. This concept is based upon the use of
expanded polypropylene (EPP) layers of foam plastic spacers in which the
mechanical and electronic boards components of the module are placed.
This pack is then housed in a metal inner cabinet which is enclosed by a
plastic external cabinet. The advantages of this packaging technology are:
• virtual elimination of fixing screws, bolts or ties, reducing the number
of components and increasing the speed of assembly/disassembly,
• the plastic layers have air channels molded into them so that cooling
air can be guided exactly to the required locations,
• the plastic layers help cushion the electronic and mechanical parts from
physical shock, and
• the metal inner cabinet shields the internal electronics from
electromagnetic interference and also helps to reduce or eliminate radio
frequency emissions from the instrument itself.

238 1260 Infinity Binary Pump User Manual

 1260 Infinity Binary Pump User Manual

14
Appendix
General Safety Information 240
The Waste Electrical and Electronic Equipment Directive 243
Lithium Batteries Information 244
Radio Interference 245
Sound Emission 246
Agilent Technologies on the Internet 247

This appendix provides general safety and environmental information.

Agilent Technologies 239

14 Appendix
General Safety Information

General Safety Information

Safety Symbols
Table 26 Safety Symbols

Symbol Description

The apparatus is marked with this symbol when the user should refer to
the instruction manual in order to protect risk of harm to the operator and
to protect the apparatus against damage.

Indicates dangerous voltages.

Indicates a protected ground terminal.

Indicates eye damage may result from directly viewing the light produced
by the deuterium lamp used in this product.

The apparatus is marked with this symbol when hot surfaces are available
and the user should not touch it when heated up.

A WARNING
WA R N I N G
alerts you to situations that could cause physical injury or death.
➔ Do not proceed beyond a warning until you have fully understood and met the
indicated conditions.

A CAUTION
CAUTION
alerts you to situations that could cause loss of data, or damage of equipment.
➔ Do not proceed beyond a caution until you have fully understood and met the
indicated conditions.

240 1260 Infinity Binary Pump User Manual

 Appendix 14
General Safety Information

General Safety Information

The following general safety precautions must be observed during all
phases of operation, service, and repair of this instrument. Failure to
comply with these precautions or with specific warnings elsewhere in this
manual violates safety standards of design, manufacture, and intended use
of the instrument. Agilent Technologies assumes no liability for the
customer’s failure to comply with these requirements.

Ensure the proper usage of the equipment.

WA R N I N G
The protection provided by the equipment may be impaired.
➔ The operator of this instrument is advised to use the equipment in a manner as
specified in this manual.

Safety Standards
This is a Safety Class I instrument (provided with terminal for protective
earthing) and has been manufactured and tested according to
international safety standards.

1260 Infinity Binary Pump User Manual 241

14 Appendix
General Safety Information

Operation
Before applying power, comply with the installation section. Additionally
the following must be observed.
Do not remove instrument covers when operating. Before the instrument is
switched on, all protective earth terminals, extension cords,
auto- transformers, and devices connected to it must be connected to a
protective earth via a ground socket. Any interruption of the protective
earth grounding will cause a potential shock hazard that could result in
serious personal injury. Whenever it is likely that the protection has been
impaired, the instrument must be made inoperative and be secured against
any intended operation.
Make sure that only fuses with the required rated current and of the
specified type (normal blow, time delay, and so on) are used for
replacement. The use of repaired fuses and the short- circuiting of fuse
holders must be avoided.
Some adjustments described in the manual, are made with power supplied
to the instrument, and protective covers removed. Energy available at
many points may, if contacted, result in personal injury.
Any adjustment, maintenance, and repair of the opened instrument under
voltage should be avoided whenever possible. When inevitable, this has to
be carried out by a skilled person who is aware of the hazard involved. Do
not attempt internal service or adjustment unless another person, capable
of rendering first aid and resuscitation, is present. Do not replace
components with power cable connected.
Do not operate the instrument in the presence of flammable gases or
fumes. Operation of any electrical instrument in such an environment
constitutes a definite safety hazard.
Do not install substitute parts or make any unauthorized modification to
the instrument.
Capacitors inside the instrument may still be charged, even though the
instrument has been disconnected from its source of supply. Dangerous
voltages, capable of causing serious personal injury, are present in this
instrument. Use extreme caution when handling, testing and adjusting.
When working with solvents, observe appropriate safety procedures (for
example, goggles, safety gloves and protective clothing) as described in the
material handling and safety data sheet by the solvent vendor, especially
when toxic or hazardous solvents are used.

242 1260 Infinity Binary Pump User Manual

 Appendix 14
The Waste Electrical and Electronic Equipment Directive

The Waste Electrical and Electronic Equipment Directive

Abstract
The Waste Electrical and Electronic Equipment (WEEE) Directive
(2002/96/EC), adopted by EU Commission on 13 February 2003, is
introducing producer responsibility on all electric and electronic
appliances starting with 13 August 2005.

This product complies with the WEEE Directive (2002/96/EC) marking requirements. The
NOTE affixed label indicates that you must not discard this electrical/electronic product in
domestic household waste.
Product Category:
With reference to the equipment types in the WEEE Directive Annex I, this product is
classed as a Monitoring and Control Instrumentation product.

Do not dispose off in domestic household waste

NOTE
To return unwanted products, contact your local Agilent office, or see www.agilent.com for
more information.

1260 Infinity Binary Pump User Manual 243

14 Appendix
Lithium Batteries Information

Lithium Batteries Information

Lithium batteries may not be disposed-off into the domestic waste. Transportation of
WA R N I N G
discharged Lithium batteries through carriers regulated by IATA/ICAO, ADR, RID,
IMDG is not allowed.
Danger of explosion if battery is incorrectly replaced.
➔ Discharged Lithium batteries shall be disposed off locally according to national
waste disposal regulations for batteries.
➔ Replace only with the same or equivalent type recommended by the equipment
manufacturer.

Lithiumbatteri - Eksplosionsfare ved fejlagtig håndtering.

WA R N I N G
Udskiftning må kun ske med batteri af samme fabrikat og type.
➔ Lever det brugte batteri tilbage til leverandøren.

Lithiumbatteri - Eksplosionsfare.
WA R N I N G
Ved udskiftning benyttes kun batteri som anbefalt av apparatfabrikanten.
➔ Brukt batteri returneres appararleverandoren.

Bij dit apparaat zijn batterijen geleverd. Wanneer deze leeg zijn, moet u ze niet weggooien
NOTE maar inleveren als KCA.

244 1260 Infinity Binary Pump User Manual

 Appendix 14
Radio Interference

Radio Interference

Cables supplied by Agilent Technologies are screened to provide optimized

protection against radio interference. All cables are in compliance with
safety or EMC regulations.

Test and Measurement

If test and measurement equipment is operated with unscreened cables, or
used for measurements on open set- ups, the user has to assure that under
operating conditions the radio interference limits are still met within the
premises.

1260 Infinity Binary Pump User Manual 245

14 Appendix
Sound Emission

Sound Emission

Manufacturer’s Declaration
This statement is provided to comply with the requirements of the
German Sound Emission Directive of 18 January 1991.
This product has a sound pressure emission (at the operator position) <
70 dB.
• Sound Pressure Lp < 70 dB (A)
• At Operator Position
• Normal Operation
• According to ISO 7779:1988/EN 27779/1991 (Type Test)

246 1260 Infinity Binary Pump User Manual

 Appendix 14
Agilent Technologies on the Internet

Agilent Technologies on the Internet

For the latest information on products and services visit our worldwide
web site on the Internet at:
http://www.agilent.com

1260 Infinity Binary Pump User Manual 247

Index

Index

8 external contacts 232 capillary

BCD absorber 14
8-bit configuration switch
board 180 changing solvents 57
without On-Board LAN 228
cable 209 cleaning 151
bench space 24 Communication settings
A
blockage 116 RS-232C 229
absorber capillary 14
board compensation sensor open 106
active inlet valve 194 HP JetDirect card 234 compensation sensor short 107
active seal wash 10, 81 boards compensation, compressibility 26
adapter 52 LAN card 234 composition accuracy 27
Agilent Lab Advisor software 97 bottle head assembly 197, 197 composition precision 27
Agilent Lab Advisor 97, 128 buffer application 61 composition range 27
Agilent buffer solutions 10 composition ripple 133
on internet 247
compressibility calibration 133
AIV fuse 113 C compressibility compensation 26, 87
algae growth 76
cable condensation 24
algae 61
analog 204 configuration
alternative seal material 82 BCD 209 low flow rates 61
ambient non-operating temperature 25 CAN 211 connections
ambient operating temperature 25 external contact 212 flow 51, 48
analog signal output 27 interface 47 control and data evaluation 27
analog signal 225 LAN 211
analog remote 206 D
cable 204 RS-232 213
damaged parts 31
apg remote 226 cables
analog 202 damper
assembly
BCD 202 when to remove 84
bottle head 197
CAN 203 data evaluation and control 27
AUTO mode 17
external contact 203 defect on arrival 30
LAN 203 delay volume 26, 84
B
overview 202 delivery checklist 31
ball-screw drive 13 remote 202 design 11
battery RS-232 203 diagnostic signals 93
safety information 244 CAN dimensions 25
BCD board cable 211 dual-piston in-series design 11

248 1260 Infinity Binary Pump User Manual

 Index

E valve failed 111 frequency range 25

wrong pump configuration 112
electrical connections
descriptions of 219
error G
solvent zero counter 109 gas solubility 61
electronic fuse of SSV open 113
exchanging gradient formation 26
electronic waste 243
inlet valve 153
electrostatic discharge (ESD) 180
interface board 180
EMF H
outlet valve sieve 153, 173
early maintenance feedback 237 outlet valve 153, 173 high-pressure mixing 10
encoder missing 117 pistons 153, 153 hints for successful use 61
error messages pump seals 153 HP JetDirect card 234
AIV fuse 113 purge valve frit 153 humidity 25
compensation sensor open 106 purge valve 153 hydraulic path with solvent selection
compensation sensor short 107 solvent selection valve 153, 177 valve 184
electronic fuse of SSV open 113 wash seals 153 hydraulic path without solvent selection
encoder missing 117 external contact valve 186
fan failed 107 cable 212 hydraulic system 26
ignition without cover 108, 108 external contacts
index adjustment 121 BCD board 232 I
index limit 120
index missing 122 index adjustment 121
F index limit 120
initialization failed 123
inlet-valve missing 117 fan failed 107 index missing 122
leak sensor open 105 filter initialization failed 123
leak sensor short 106 solvent inlet 61 initialization 14
leak 105 firmware injection precision 80
lost CAN partner 104 description 216 inlet valve body 153
missing pressure reading 112 main system 216 inlet-valve missing 117
motor-drive power 116 resident system 216
installation
overpressure 128, 134, 132, 136 update tool 217
bench space 24
pressure above upper limit 109 updates 217, 181, 181
instrument layout 238
pressure below lower limit 110 upgade/downgrade 181
interface board 180
pressure signal missing 110 upgrade/downgrade 181
interface cable 47
pump head missing 119 flow accuracy 26
remote timeout 104 interfaces 221
flow connections 48, 51
servo restart failed 118 internet 247
flow precision 26
shutdown 103 introduction to the pump 10
flow range 10
stroke length 122 operational 26
temperature limit exceeded 115 setable 26 L
temperature out of range 114 flow rate LAN
timeout 102 minimum 61 cable 211

1260 Infinity Binary Pump User Manual 249

Index

communication interface board 234 outlet valve 153, 173, 192 pulsation 26
leak rate test overpressure error 128, 132, 134, 136 priming
evaluating results 140 with a pump 56
potential causes of failure 141 P PTFE frit 153
running the test 139 pulsation
packaging
leak sensor open 105 damaged 30 pressure 17
leak sensor short 106 parts identification pump elasticity calibration 93, 136
leak 105 cables 201 pump head assembly 153
line frequency 25 parts pump head missing 119
line voltage 25 active inlet valve 194 pump head 153, 153
liquimeter 162 bottle head assembly 197 pump seals
lithium batteries 244 damaged 31 alternative material 82
lost CAN partner 104 hydraulic path with solvent selection for normal phase solvents 82
valve 184 purge valve 61, 153
M hydraulic path without solvent
selection valve 186 R
main assemblies, overview 152 missing 31
maintenance outlet valve 192 radio interference 245
feedback 237 system tool kit 196 reassembling the pump head 167
pump head with seal wash 153 performance recommended pH range 26
pump head without seal wash 153 specifications 26 remote
replacing firmware 181, 181 cable 206
pH range 26
message remove damper and mixer 84
physical specifications 25
ignition without cover 108, 108 removing the pump head 157
piston 13
remote timeout 104 removing
power consideration 22
minimum flow rate 61 pump head assembly 153
power consumption 25
missing parts 31 repair procedures 153
power cords 23
missing pressure reading 112 repairs
power supply indicator 94
mixer 11 definition of 148
power switch 46
when to remove 84 introduction 148
pressure above upper limit 109
motor-drive power 116 replacing firmware 181, 181
pressure below lower limit 110
warnings and cautions 148
N pressure pulsation 17, 88
results
pressure range 82 pressure test 132, 129
non-operating altitude 25
pressure ripple 133 ripple
non-operating temperature 25
pressure signal missing 110 composition 133
pressure test 92 pressure 133
O
results 132 RS-232C
operating Altitude 25 pressure cable 213
operating temperature 25 operating range 26 communication settings 229

250 1260 Infinity Binary Pump User Manual

 Index

S special interfaces 227 U

special settings
safety class I 241 unpacking the pump 30
boot-resident 231
safety information
forced cold start 231
lithium batteries 244 V
specification
safety vacuum degasser 10, 27, 61, 61
physical 25
general information 241 operational hints 80
specifications
standards 25 when to use 80
analog signal output 27
symbols 240 valve failed 111
communications 27
sapphire piston 13 valve
control and data evaluation 27
seal wash inlet valve, replacement 153
performance 26
wash solvent 81 solvent selection 177
stack configuration
when to use 81 variable reluctance motor 14
low delay volume with post column
seal wear counter 162 cooler 38 variable stroke volume 17
seals low delay volume 37 voltage range 25
alternative material 82 medium delay volume 35
normal phase 82 standard delay volume 33 W
servo restart failed 118 status indicator 95
wash solvent 81
setable flow range 26 stroke length 122
waste
shutdown 103 stroke volume 14, 17 electrical and electronic
sieve 153, 173 system tool kit 196 equipment 243
simple repair procedures 153
WEEE directive 243
site requirements 21 T weight 25
power cords 23
temperature limit exceeded 115 wrong pump configuration 112
snap fastener 48, 51
temperature out of range 114
solubility, gas 61
temperature sensor 105
solvent cabinet 49, 61
test functions 92
solvent calibration 133
tests
solvent compressibility calibration 93,
pressure test results 129
134
pressure test 128
solvent filters
pump elasticity calibration 136
checking 77
solvent calibration 133
cleaning 77
solvent compressibility
prevent blocking 76 calibration 134
solvent inlet filter 61 system pressure test 128
solvent selection valve 10, 48, 153, timeout 102
177
troubleshooting
solvent zero counter 109 error messages 92, 101
solvents, change 57 status indicators 94, 92
sound emission 246

1260 Infinity Binary Pump User Manual 251

www.agilent.com

In This Book

This manual contains technical reference

information about the Agilent 1260 Infinity
Binary Pump G1312B. The manual describes
the following:
• introduction,
• site requirements and specifications,
• installing the pump,
• using the binary pump,
• optimizing performance,
• troubleshooting and diagnostics,
• maintenance,
• parts and materials for maintenance,
• identifying cables,
• hardware information,
• appendix.

© Agilent Technologies 2005-2008, 2010-2013

Printed in Germany
11/2013

*G1312-90015*
*G1312-90015*
G1312- 90015
Rev. B

Agilent Technologies