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Pharmaceutical Microbiology Laboratory Worksheet UST - Faculty of Pharmacy

The document is a laboratory worksheet for a pharmaceutical microbiology class. It contains scenarios analyzing aseptic techniques and inoculation of media. For each scenario, the student identifies the possible effect of improper procedures and provides a rationale. The scenarios cover issues like using filters of unknown pore size, flame sterilizing a loop that already contains bacteria, leaving UV light on near samples, insufficient oven sterilization time and temperature, and oven sterilizing aqueous culture medium. The worksheet also instructs the student to draw step-by-step diagrams demonstrating proper aseptic technique for transferring broth culture to plated and tubed solid media.

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PRINCESS JULIENNE YU
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480 views3 pages

Pharmaceutical Microbiology Laboratory Worksheet UST - Faculty of Pharmacy

The document is a laboratory worksheet for a pharmaceutical microbiology class. It contains scenarios analyzing aseptic techniques and inoculation of media. For each scenario, the student identifies the possible effect of improper procedures and provides a rationale. The scenarios cover issues like using filters of unknown pore size, flame sterilizing a loop that already contains bacteria, leaving UV light on near samples, insufficient oven sterilization time and temperature, and oven sterilizing aqueous culture medium. The worksheet also instructs the student to draw step-by-step diagrams demonstrating proper aseptic technique for transferring broth culture to plated and tubed solid media.

Uploaded by

PRINCESS JULIENNE YU
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
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Download as PDF, TXT or read online on Scribd
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PHARMACEUTICAL MICROBIOLOGY LABORATORY WORKSHEET

UST – Faculty of Pharmacy

Group #8 Member #____ Class: #43


Name: Princess Julienne Y. Yu Section: 2GPH

EXERCISE 1
ASEPTIC TECHNIQUES AND INOCULATION OF MEDIA

Laboratory Scenario Analysis: Read the following statements. Fill-in the possible effect of the
procedure and give the rationale.

1. Heat-labile material to be introduced to culture medium was filtered using membrane


filters with unknown pore size.

Effect: This will contaminate your culture medium because the unknown filter that you used
might have large pores that the contaminants or bacteria will easily pass through.

Rationale: When using the filtration technique, it is necessary to use filters that have the pore sizes
of 0.45 micrometer and 0.22 micrometer to ensure the sterilization of the fluids.

2. Loop with bacterial inoculum was subjected to flame sterilization prior to streaking
on agar plate.

Effect: Subjecting the loop that contains bacterial inoculum to open flame will kill the
microorganisms.

Rationale: Flame sterilization is utilized in the laboratory to ensure the sterilization of the
inoculating loops prior to the transferring of microorganisms. The step stated above
should be done to the loop before subjecting it to the bacterial inoculum.

3. UV light in biosafety cabinet was left “turned on” while samples for product testing
were being prepared.

Effect: The UV light would affect and kill the samples of living microorganisms.

Rationale: Important samples of living microorganisms should be protected or sheltered from UV


light as its harmful rays serves as a sterilization process for the room. UV light could also
harm the person preparing the samples.

4. Oven sterilization at 1210C was performed on glassware for 15 minutes only.

Effect: This would not properly sterilize the glassware and will lead to contamination on your future
experiments.

Rationale: This would not be effective since the desired or proper temperature of the oven needs to
be at 160 degrees Celsius for not more than 2 hours or 170 degrees Celsius for exactly an
hour.

5. Lid of petri dish was left on bench top for a few minutes after pouring of sterile
medium.

Effect: The lid being left unattended would cause cross contamination and will tamper with the
results.

Rationale: This can cause contamination because of its exposure to its surroundings that positively
carries bacteria.

6. Oven sterilization of aqueous culture medium.

Effect: The oven sterilization is not designed for aqueous culture medium as this can damage your
microorganism samples.

Rationale: The proper way to sterilize aqueous culture medium is by autoclaving. If oven
sterilization is used, the dry heat will cause evaporation that can lead to the sample
drying or burning of cells.
PHARMACEUTICAL MICROBIOLOGY LABORATORY WORKSHEET

UST – Faculty of Pharmacy

Inoculation of Media: Make illustrated diagrams of stepwise procedure for the performance of the
following tasks applying appropriate aseptic techniques.
A. Transfer of Broth Culture to Plated Solid Media

B. Transfer of Broth Culture to Tubed Solid Media

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