U2020 PC Control Software

UVmate User’s Manual

Irmeco Germany
Version 3.40
 Table of Contents

Chapter 1 Installation 1
Chapter 2 Start Connection 1
Chapter 3 Function Lambda 5
Chapter 4 Setup of Accessories 6
Chapter 5 Function Photometry 8
Chapter 6 Function Spectrum 13
Chapter 7 Function Timescan 22
Chapter 8 Function Kinetic 27
Chapter 9 Function Quantitative 32
Chapter 10 Function System Setup 37
Chapter 11 Function Applications-DNA_RNA 42
Chapter 12 Function Applications-Protein Analysis 45
Chapter 13 Other Functions 48
Appendix A UV mate Installation and Operation 50

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Chapter 1 Installation

1. System requirements:
z IBM compatible with CPU Pentium-100 or above, equipped with CD-ROM
z Microsoft Windows 98(SE) or XP
z 16M RAM and 20Mbytes hard disk space for installing UVmate program
z Display card supporting 800X600 resolution, 16bit high color, or above
z Mouse, keyboard, RS-232 serial com port (usually COM2), and a 9-pin
connection line (one male and one female with all pins wired)
2. From CD ROM, select Setup.exe in UVmate directory and execute it (skip this
step if it has been done before).
3. Follow instructions to go through installation. Usually all files will be installed
under directory ‘C:\programfiles\UVmate’ and the executable file is UVmate.exe.
Several file folders will be created for storing data files. There are sample files in
each folder so that UVmate can be run without connection to physical
instrument.

Chapter 2 Start Connection

1. Turn on the power of U2020 instrument. After 30 seconds, the LCD monitor of
instrument will show message ‘Waiting for connecting …’, which means that the
instrument has completed the power-on procedure and is waiting for connection
with PC.
2. In the Program files under ‘Start’ menu of windows, there is a short-cut named
UVmate under ‘Irmeco’. Click it and UVmate.exe can be executed.

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3. After the program executed, a dialog box ‘COM Port Setting’ will show up. The
default setting is COM2 and user can select whichever is suitable in real
circumstances. Before connection, the followings should be checked:
a. The RS-232 connection line should be connected between PC and
U2020. The connection line should have 9-pin connectors with all pins
wired.
b. U2020 should be in the condition for waiting connection. Messages
‘Version 1.3 for PC’ and ‘Waiting for connecting …’ should be shown
on the monitor. If there is no message, which means not in the
connection mode, please turn off the power of U2020. Hold on the
ESC key and turn on the power of U2020 again; release the ESC key
after hearing two beep sounds. It will switch U2020 into connection
mode. It also switches U2020 into the single mode.
c. The default settings for COM Port are: Baud rate=57600, 8 bits transfer,
no parity check, and 1 stop bit (in short, 57600,8,N,1).

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4. Press ‘Port Open’ button and the status bar will change ‘Off-Line’ into ‘On-Line’.
It represents a successful connection.
5. After successful connection, the status bar will show the current wavelength and
the reading of U2020. Data from U2020 is transferred into PC continuously. User
can see ‘OK’ showing in the status bar and ‘…’ keeps running during connection
between instrument and PC.

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6. The window of software shows, from the above, program title, menu bar, toolbar,
status bar, and the rest of area for datum. Some explanations are as follows:
a. ‘File’ of menu bar has options for ‘Open parameter file’, ‘Open data
file’, ‘Save parameter file’, ‘Save data file’, ‘Report preview’, ‘Report
print’, ‘Export to Excel’, and ‘Exit’.
b. ‘Setting’ of menu bar has ‘Com port …’ for opening dialog box of
communication port.
c. ‘‘Function’ of menu bar has different functions for selection:
Photometry, Spectrum, Time scan, Kinetic, Quantitative, Applications,
and System. In option ‘Applications’, there are listed sub functions for
various applications. In option ‘System’, there are sub functions:
‘Property Setup’, ‘Verify Bandwidth’ for activating function in
verifying 656.1nm, and ‘Temperature Monitor’ for activating monitor
function in temperature changing.
d. ‘Help’ of menu bar has ‘About’ for introduction of this program.
e. Icons in toolbar, from left to right, are: 'Parameter setup',
'Zero/Baseline', 'Data reading', 'Stop data reading', 'Auto zoom data' for
auto scaling datum, 'Zoom in/out data', 'Original data' for restoring
original data scale, 'Peak/valley search', 'Data smoothing', 'Data
derivative', 'Data manipulation', 'Color setup' for assigning color for
different data segment, 'Report preview', and 'Report print'.
f. Fields in status bar, from left to right, are ‘Present wavelength’,
‘Present data’ for current reading, ‘Lambda parameter setup’,
‘Connecting status’, ‘Com port setup’, ‘Accessory status’, ‘Accessory
setup’ for opening dialog box for setting up accessories, and ‘Present
status’ for the instrument.

7. If the connection is not successful, the status bar remains in ‘Off-line’ and this
program will be running as a demo version. It can only read Sample files under
such condition.

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Chapter 3 Function Lambda

1. Press the λ button in the status bar, a window for function Lambda will show
in the screen. Some explanations of this function are as follows.
2. Field ‘Wavelength(nm)=’ is used for key in the desired value of wavelength. The
input value of wavelength will be recorded in the current wavelength field in
order to pick up rapidly by using mouse.
3. Field ‘Measure mode=’ is for selecting the proper unit during measurement and
this field can not accept input from keyboard.
4. Field ‘Factor=’ will be enabled (turning into white) for accepting key-in value
when ‘Factor*ABS’ is chosen in ‘Measure mode’ field.
5. The ‘Goto lambda’ button will accept the key-in value in ‘Wavelength’ field and
will run the instrument to the desired position.
6. The ‘Zero’ button will force the instrument to perform the zeroing function.
7. The ‘Toggle digits’ button will switch digit numbers for the current measurement;
for example, 0.012A→0.0124A.
8. The ‘Exit’ button will close the Lambda function.
9. After close the Lambda function, the status bar will still show the current
wavelength and the corresponding reading in a real-time manner.

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Chapter 4 Setup of Accessories

1. Press button in the status bar, a window for Setup accessory will
show in the screen if the instrument is equipped with extra accessory (Multi Cell,
Sipper, or Temperature Controller, etc). Some explanations of functions in this
window are as follows.
2. The above dialog box will show up if a Multi cell is present. Select directly in
field ‘Run cell to [1-6]=’ and the Multi cell will go to the required position.
Pressing ‘Cell+’ button will make the Multi cell moving one cell forward while
pressing ‘Cell-’ button backward. Pressing ‘Cell home’ button will make the
Multi cell back to Cell 1 while pressing ‘Cell standby’ button to Cell 6 in order to
load cells by user.

3. The above dialog box will show up if a Temperature controller is present. Select
directly in field ‘Temperature Controller(C)=’ and the desired temperature will be
set by instrument. Selecting ‘None’ means no temperature control. Be aware that
direct value key-in is an invalid movement.
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4. The above dialog box will show up if a peristaltic pump ‘Sipper’ is present. Field
‘Pump speed=’ is to set up speed in Sipper. Field ‘Pre air purge time=’ is to set up
time for pre air purge in Sipper. Field ‘Air purge time=’ is to set up time for air
purge in Sipper. Field ‘Sipper time=’ is to set up sipping time for samples. Field
‘Dwell time=’ is to set up time for sample staying in the Sipper. The ‘Sipper test’
button is for testing if all setup values are correct. The ‘Sipper wash’ button is for
washing the chamber of Sipper and the washing time is set as 15 seconds.

5. The above dialog box will show up if both Sipper and Temperature Controller
(TC) are present. Operations are similar to those in Sipper but adding a field
‘T-Controller(C)=’ for temperature control.

The field ‘Accessory status’ on the status bar will show messages accordingly:
Single cell: NA in the field as for no accessory available (single cell only)
Singer cell + TC: current temperature; for example, T=25C.
Multi cell: current position of cell holder; for example, 5.
Sipper: Sipper in the field.
Sipper + TC: Sipper and current temperature; for example, Sipper-T=25C.

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Chapter 5 Function Photometry

1. Choose ‘Photometry’ from ‘Function’ of menu bar and a screen for function
Photometry will show up. A dialog box of ‘Setup Photometry’ also shows up.
Press ‘OK’ after setting to execute the program according to new parameters. Or
press ‘Cancel’ to cancel settings and the program runs according to old
parameters. There are four tabs for setting in this screen: Instrument, Option,
Accessory, and Report.
a. Tab ‘Instrument’ refers to basic parameters of this function. More details are
described below:
z ‘Number of Lambda’ represents the desired number of wavelength for
measurement.
z Input the desired wavelength according to ‘Number of Lambda’.
z In ‘Measure Mode’, there are three choices: ABS, %T, or Factor*ABS.
z In ‘Unit’, user can select the unit. It is functional only when Measure
Mode= Factor*ABS.
z In ‘Factor’, user can key in the value of factor. It is functional only
when Measure Mode= Factor*ABS.
z ‘Cycle Number’ sets up total number of cycle for measurement.
z ‘Cycle interval (sec)’ defines the interval of time every cycle.

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b. Tab ‘Option’ is not available for this function.
c. Tab ‘Accessory’ shows ‘The accessory is not available’ when only Single
cell is used. When other accessories exist, this field will show messages
according to screen of ‘Setup accessory’.

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d. Tab ‘Report’ is to set up options for printing reports in this function.
z ‘Laboratory’ is for input of user’s company or name of laboratory.
z ‘Subject’ is for input of the subject of this experiment.
z ‘Operator’ is for input of user’s name or comprehensive code.
z ‘Remark’ is for input of relative remarks.
z ‘Print present date’ will print the current date in computer.
z ‘Print page number’ will print the page number for each page.
z ‘Print parameter setup’ will print all settings for parameters.
z ‘Print selected data’ will print selected data of readings (marked with
‘v’).

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2. After setting up parameters for function Photometry, press icon in toolbar
and program will start to read and to record data accordingly. Symbol ‘v’ after
serial numbers in table means that the data will be printed in report. If user
doesn’t want the data in the report, please click left button of mouse in that field
and the symbol will change into ‘x’.

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3. Press icon in the toolbar and user can preview the report for printing. In

the screen, icon can zoom in the report and icon can zoom out the

report.

4. In the toolbar, press icon to execute function of printing the report. During
the printing process, the default printer will be selected by system. If there is no
printer in the system, an error message will show up.
5. The contents of report for Photometry include report headers, settings of
parameters, and selected measurements.

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Chapter 6 Function Spectrum

1. Choose ‘Spectrum’ from ‘Function’ of menu bar and a screen for function
Spectrum will show up. A dialog box of ‘Setup Spectrum’ also shows up. Press
‘OK’ after setting to execute the program according to new parameters. Or press
‘Cancel’ to cancel settings and the program runs according to old parameters.
There are four tabs for setting in this screen: Instrument, Option, Accessory, and
Report.
a. Tab ‘Instrument’ refers to basic parameters of this function. More details are
described below:
z ‘Start Wavelength(nm)’ represents the start wavelength for
measurement.
z ‘Stop Wavelength(nm)’ represents the stop wavelength for
measurement.
z In ‘Measure Mode’, there are two choices: ABS, and %T.
z In ‘Low Value(%T)’, user can set up the low value in scale of graph
and the unit will change depending on ‘Measure mode’.
z In ‘High Value(%T)’, user can set up the high value in scale of graph
and the unit will change depending on ‘Measure mode’.
z ‘Scan Speed(nm/min)’ represents the speed in scan.

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b. Tab ‘Option’ refers to special options. Details are described below.
z ‘Auto zoom in/out data’ will zoom datum to fit in the optimal scale
automatically after reading data.
z ‘Auto search peak point’ will search for ‘Peak’ automatically after
reading data.
z ‘Auto search valley point’ will search for ‘Valley’ automatically after
reading data.
z ‘Overlay screen’ will not clear previous readings when doing multiple
scan.
c. Tab ‘Accessory’ shows ‘The accessory is not available’ when only Single
cell is used. When other accessories exist, this field will show messages
according to screen of ‘Setup accessory’.

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d. Tab ‘Report’ is to set up options for printing reports in this function.
z ‘Laboratory’ is for input of user’s company or name of laboratory.
z ‘Subject’ is for input of the subject of this experiment.
z ‘Operator’ is for input of user’s name or comprehensive code.
z ‘Remark’ is for input of relative remarks.
z ‘Print present date’ will print the current date in computer.
z ‘Print page number’ will print the page number for each page.
z ‘Print parameter setup’ will print all settings for parameters.
z ‘Print track data’ will print selected data in track (marked with ‘v’).
z ‘Print peak data’ will print selected data of peaks (marked with ‘v’).
z ‘Print valley data’ will print selected data of valleys (marked with ‘v’).

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2. After setting up parameters for function Spectrum, press icon in the toolbar
and program will start reading datum and will show those in a graph. After
readings, user can select desired area in graph by holding down left button of
mouse, moving the mouse, and then releasing the left button. The selected area of
graph will be zoomed in automatically.
3. Moving mouse to any position in the graph and then hitting right button of the
mouse, this can record data of that point to the field ‘Track data’. Every record
will be numbered accordingly.

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4. Press icon in the toolbar and user can manually zoom in or out data
according to parameter setting. This function can perform precise work in zoom.

5. Press icon in the toolbar and user can restore all data to the original scale.

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6. In the toolbar, press icon to execute function for searching Peaks and
Valleys automatically. All details of searching results will be recorded in the data
area to the right.

7. In the toolbar, press icon to execute function for smoothing data. There are
three ways for smoothing data - Smooth low, Smooth middle, and Smooth high.
The difference between those three ways is the total number of data points used
for calculation.

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8. Press icon in the toolbar and a dialog box of Set color will show up. In the
box, all scanned data segments will be shown. For those to be shown in the graph,
mark ‘v’ in the ‘Visible’ option for each segment. If special treatment is needed
for specific data segment, mark ‘‧’ in the ‘Active’ option field. Such that, when
performing functions like Zoom, Peak/Valley, or Track and so on, it will take the
‘Active’ segment as the base. If there are too many data segments, press ‘Reset
Curve’ button to reset the numbering system.

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9. Press icon in the toolbar and user can preview the report for printing. In the

screen, icon can zoom in the report and icon can zoom out the report.

10. In the toolbar, press icon to execute function for taking derivative for data.
First to third derivative can be calculated and the derived spectrum will be
displayed in a separate figure right beneath the original figure.

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11. Press icon in the toolbar and a dialog box for spectrum manipulation will
show up. Users can manipulate existing spectra to get new spectrum. In the box,
all spectra will be identified by pre-determined colors (up to 20 spectra). Those
spectra can be added, subtracted, or multiplied through the equation:

New scan = (Scan I × C1) +/-/* (Scan J × C2) +/-/* C3

where Scan I and Scan J are known spectrum data, C1 and C2 are corresponding
coefficients for Scan I and Scan J, separately. And C3 is an additional factor for
the new spectrum.

12. In the toolbar, press icon to execute function of printing the report. During
the printing process, the default printer will be selected by system. If there is no
printer in the system, an error message will show up.
13. The contents of report for Spectrum include report headers, graphs, settings of
parameters, and tables for Track data, Peak data, and Valley data.

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Chapter 7 Function Timescan

1. Choose ‘Timescan’ from ‘Function’ of menu bar and a screen for function
Timescan will show up. A dialog box of ‘Setup Timescan’ also shows up. Press
‘OK’ after setting to execute the program according to new parameters. Or press
‘Cancel’ to cancel settings and the program runs according to old parameters.
There are four tabs for setting in this screen: Instrument, Option, Accessory, and
Report.
a. Tab ‘Instrument’ refers to basic parameters of this function. More details are
described below:
z ‘Wavelength(nm)’ represents the wavelength for measurement.
z ‘Scan Time(sec)’ represents the time needed for measurement.
z ‘Delay Time(sec)’ represents the delay time before taking readings.
z In ‘Measure Mode’, there are two choices: ABS, and %T.
z In ‘Low Value(%T)’, user can set up the low value in scale of graph
and the unit will change depending on ‘Measure Mode’.
z In ‘High Value(%T)’, user can set up the high value in scale of graph
and the unit will change depending on ‘Measure Mode’.

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b. Tab ‘Option’ refers to special options. Details are described below.
z ‘Auto zoom in/out data’ will zoom datum to fit in the optimal scale
automatically after reading data.
z ‘Overlay screen’ will not clear previous readings when doing multiple
scan.
c. Tab ‘Accessory’ shows ‘The accessory is not available’ when only Single
cell is used. When other accessories exist, this field will show messages
according to screen of ‘Setup accessory’.

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d. Tab ‘Report’ is to set up options for printing reports in this function.
z ‘Laboratory’ is for input of user’s company or name of laboratory.
z ‘Subject’ is for input of the subject of this experiment.
z ‘Operator’ is for input of user’s name or comprehensive code.
z ‘Remark’ is for input of relative remarks.
z ‘Print present date’ will print the current date in computer.
z ‘Print page number’ will print the page number for each page.
z ‘Print parameter setup’ will print all settings for parameters.
z ‘Print track data’ will print selected data in track (marked with ‘v’).

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2. After setting up parameters for function Timescan, press icon in the toolbar
and program will start reading datum and will show those in a graph. After
readings, user can select desired area in graph by holding down left button of
mouse, moving the mouse, and then releasing the left button. The selected area of
graph will be zoomed in automatically.
3. Moving mouse to any position in the graph and then hitting right button of the
mouse, this can record data of that point to field ‘Track data’. Every record will
be numbered accordingly.
4. In this function, operations for ‘Zoom’, ‘Smooth’, and ‘Set color’ are the same of
those in function Spectrum.

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5. Press icon in the toolbar and user can preview the report for printing. In the

screen, icon can zoom in the report and icon can zoom out the report.

6. In the toolbar, press icon to execute function of printing the report. During
the printing process, the default printer will be selected by system. If there is no
printer in the system, an error message will show up.
7. The contents of report for Timescan include report headers, graphs, settings of
parameters, and table for Track data.

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Chapter 8 Function Kinetic

1. Choose ‘Kinetic’ from ‘Function’ of menu bar and a screen for function Kinetic
will show up. A dialog box of ‘Setup Kinetic’ also shows up. Press ‘OK’ after
setting to execute the program according to new parameters. Or press ‘Cancel’ to
cancel settings and the program runs according to old parameters. There are four
tabs for setting in this screen: Instrument, Option, Accessory, and Report.
a. Tab ‘Instrument’ refers to basic parameters of this function. More details are
described below:
z ‘Wavelength(nm)’ represents the wavelength for measurement.
z ‘Sampling Number’ represents the number for samples.
z ‘Lag Time(sec)’ represents the lag time needed in measurement.
z ‘Rate Time(sec)’ represents the rate time needed in measurement.
z ‘Delay Time(sec)’ represents the delay time before taking readings.
z In ‘Low Value(ABS)’, user can set up the low value in scale of graph
and the unit is ABS.
z In ‘High Value(ABS)’, user can set up the high value in scale of graph
and the unit is ABS.
z In ‘Factor’, user can set up value for factor.
z In ‘Unit’, user can select different unit as necessary.

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b. Tab ‘Option’ refers to special options. Details are described below.
z ‘Auto zoom in/out data’ will zoom datum to fit in the optimal scale
automatically after reading data.
c. Tab ‘Accessory’ shows ‘The accessory is not available’ when only Single
cell is used. When other accessories exist, this field will show messages
according to screen of ‘Setup accessory’.

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d. Tab ‘Report’ is to set up options for printing reports in this function.
z ‘Laboratory’ is for input of user’s company or name of laboratory.
z ‘Subject’ is for input of the subject of this experiment.
z ‘Operator’ is for input of user’s name or comprehensive code.
z ‘Remark’ is for input of relative remarks.
z ‘Print present date’ will print the current date in computer.
z ‘Print page number’ will print the page number for each page.
z ‘Print parameter setup’ will print all settings for parameters.
z ‘Print track data’ will print selected data in track (marked with ‘v’).
z ‘Print sampled data’ will print selected data in samples (marked with
‘v’).
z ‘Print output data’ will print selected data for output (marked with ‘v’).

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2. After setting up parameters for function Kinetic, press icon in the toolbar
and program will start reading datum and will show those in a graph. After
measurement, program will find data for Sampling point and will record in the
field ‘Sampled data’ automatically. Note that those data in this area are always
marked with ‘v’ and can not be altered partially for printing by user.
3. For every measurement, program will store data like Lambda, ABS0, … … etc. in
the field of ‘Output data’.
4. Moving mouse to any position in the graph and then hitting right button of the
mouse, this can record data of that point to field ‘Track data’. Every record will
be numbered accordingly.

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5. Press icon in the toolbar and user can preview the report for printing. In the

screen, icon can zoom in the report and icon can zoom out the

report.

6. In the toolbar, press icon to execute function of printing the report. During
the printing process, the default printer will be selected by system. If there is no
printer in the system, an error message will show up.
7. The contents of report for Kinetic include report headers, graphs, settings of
parameters, and tables for Track data, Sampled data, and Output data.

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Chapter 9 Function Quantitative

1. Choose ‘Quantitative’ from ‘Function’ of menu bar and a screen for function
Quantitative will show up. A dialog box of ‘Setup Quantitative’ also shows up.
Press ‘OK’ after setting to execute the program according to new parameters. Or
press ‘Cancel’ to cancel settings and the program runs according to old
parameters. There are four tabs for setting in this screen: Instrument, Standard,
Accessory, and Report.
a. Tab ‘Instrument’ refers to basic parameters of this function. More details are
described below:
z ‘Wavelength(nm)’ represents the wavelength for measurement.
z In ‘Low Value(ABS)’, user can set up the low value in scale of graph
and the unit is ABS.
z In ‘High Value(ABS)’, user can set up the high value in scale of graph
and the unit is ABS.
z In ‘Unit’, user can select different unit as necessary.

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b. Tab ‘Standard’ refers to options for setting up standard. Details are described
below.
z Three standard types for user to choose: ‘Known Conc. and read ABS’
for known concentrations with unknown ABS for measurement,
‘Known Conc. and keyin ABS’ for known concentrations with
user-decided ABS to key-in, and ‘Known factor’ for known factor.
z Four fit types for user to choose; from left to right, 1st order equation
passing origin, 1st order equation without passing origin, 2nd order
equation passing origin, and 2nd order equation without passing origin.
z ‘Standard number’ is for user to input number of standards.
z Followed by the above three selections, user will key in proper values
for standards.
c. Tab ‘Accessory’ shows ‘The accessory is not available’ when only Single
cell is used. When other accessories exist, this field will show messages
according to screen of ‘Setup accessory’.

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d. Tab ‘Report’ is to set up options for printing reports in this function.
z ‘Laboratory’ is for input of user’s company or name of laboratory.
z ‘Subject’ is for input of the subject of this experiment.
z ‘Operator’ is for input of user’s name or comprehensive code.
z ‘Remark’ is for input of relative remarks.
z ‘Print present date’ will print the current date in computer.
z ‘Print page number’ will print the page number for each page.
z ‘Print parameter setup’ will print all settings for parameters.
z ‘Print standard data’ will print selected data of standards (marked with
‘v’).

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2. After setting up parameters for function Quantitative, press icon in the
toolbar to run the function. If standard type ‘Known conc. and read ABS’ is
selected, it will start to measure standards and set up the standard table.
Otherwise, it can start to measure samples.
3. Data of standards will be in field ‘Standard data’. Note that those data in this area
are always marked with ‘v’ and can not be altered partially for printing by user.
4. For every measurement, program will store data in field ‘Sample data’.

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5. Press icon in the toolbar and user can preview the report for printing. In

the screen, icon can zoom in the report and icon can zoom out the

report.

6. In the toolbar, press icon to execute function of printing the report. During
the printing process, the default printer will be selected by system. If there is no
printer in the system, an error message will show up.
7. The contents of report for Quantitative include report headers, graphs, settings of
parameters, and tables for Standard data and Sampled data.

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Chapter 10 Function System Setup

1. Under ‘System Setup’ from ‘Function’ of menu bar, select ‘Verify Bandwidth’
and a function screen ‘Verify Bandwidth’ will show up.

2. In screen ‘Verify Bandwidth’, press icon in the toolbar and this function
will be executed. If the system is in the normal condition, the value of
‘Bandwidth’ should be 2.0nm±0.2nm and the value of ‘Lambda(max)’ should be
656.1nm±1nm.

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3. Under ‘System Setup’ from ‘Function’ of menu bar, select ‘Property Setup’ and a
function screen ‘Property Setup’ will show up. There are two tabs for setting in
this screen: Baseline and Option.
a. In tab ‘Baseline’, some explanations of this function are described below:
z ‘Save data after measure baseline’ represents that data will be saved in
file automatically after the measurement for Baseline.
z Press ‘Measure baseline’ button and the instrument will perform
measurement for Baseline. In order to get stable readings, turn on the
instrument one hour before this measurement.
z Press ‘Save baseline’ button and the current data of Baseline will be
saved in file.
z Press ‘Load baseline from file’ button and the data of Baseline in file
will be read. It is useful to use this function when user can not get the
correct Baseline in Spectrum function.
z Press ‘Search zero position’ button and the instrument will run into the
default position for optical mechanism. User can inspect a rectangular
light spot in the space for cell holder.
z Doing ‘Search zero position’ before ‘Measure baseline’ will make
readings for baseline more stable.

38
b. In tab ‘Option’, some explanations of this function are described below:
z ‘UV/Vis switching wavelength[nm]=’ in ‘Hardware’ allows user to
change the switching position for UV lamp and Tungsten lamp.
z ‘Switch to single cell’ in ‘Hardware’ represents that the instrument
conducts mode as in single cell when there is accessory; like Multi cell,
Sipper, etc. The above requests will not be activated until ‘Send
request’ is pressed.
z ‘Use UV lamp’ in ‘Lamp’ is for user to choose the UV lamp of the
instrument to be on or not. This request will not be activated until ‘Send
request’ is pressed.
z Message ‘Visible lamp status = …’ in ‘Lamp’ represents the
accumulated time being used of the current visible lamp.
z Message ‘UV lamp status = …’ in ‘Lamp’ represents the accumulated
time being used of the current UV lamp.
z Button ‘Reset Vis hour’ in ‘Lamp’ can set the accumulated time of the
visible lamp as zero when changing the visible lamp.
z Button ‘Rest UV hour’ in ‘Lamp’ can set the accumulated time of the
UV lamp as zero when changing the UV lamp.

39
z When the dialog box of ‘System Setup’ activated, the usage time of
lamps will be read once. If user wants to observe the usage time of
lamps later, ‘Send request’ in ‘Lamp’ should be pressed again for a
newly accumulated usage time.

40
4. Under ‘System Setup’ from ‘Function’ of menu bar, select ‘Temperature Monitor’
and a function screen ‘Temperature Monitor’ will show up. This function screen
allows user to monitor how long it takes for the instrument to get the stable
temperature when user changes the setting for temperature control.

5. In the screen, press icon in the toolbar and it will take records of
temperature for about 280 seconds.

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Chapter 11 Function Applications – DNA_RNA

1. Select ‘DNA_RNA’ in ‘Applications’ and a function screen ‘DNA & RNA’ will
show up. A dialog box of ‘Setup DNA & RNA’ also shows up. Press ‘OK’ after
setting to execute the program according to new parameters. Or press ‘Cancel’ to
cancel settings and the program runs according to old parameters. There are four
tabs for setting in this screen: Instrument, Option, Accessory, and Report.
a. Tab ‘Instrument’ refers to basic parameters in this application. More details
are described below:
● In ‘Assay type’, there are five choices. They are:
260nm/280nm Ratio:No Bkg
260nm/280nm Ratio:Bkg=320nm
230nm/260nm Ratio:Bkg=320nm
Warburg-Christian Concentration
General Ratio and Concentration
In ‘Warburg-Christian Concentration’, the formula for calculating
concentrations of ‘Protein’ and ‘Nucleic Acid’ are defined according to
Warburg-Christian algorithms as shown in the screen.
In ‘General Ratio and Concentration’, user can determine all necessary

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 values for wavelengths and coefficients to set up special formula for
calculating concentrations of ‘Protein’ and ‘Nucleic Acid’.
● ‘Delay time(sec)’ represents the delay time before taking readings.
● ‘Cycle number’ sets up total number of cycle for measurements.
z ‘Cycle interval(sec)’ defines the interval of time every cycle.

b. Tab ‘Option’ and ‘Accessory’ are not available in this application.

c. ‘Report’ is to set up options for printing reports in this application.
z ‘Laboratory’ is for input of user’s company or name of laboratory.
z ‘Subject’ is for input of subject of this experiment.
z ‘Operator’ is for input of user’s name or comprehensive code.
z ‘Remark’ is for input of relative remarks.
z ‘Print present date’ will print the current date in computer.
z ‘Print page number’ will print the page number for each page.
z ‘Print parameter setup’ will print all settings for parameters.
z ‘Print selected data’ will print selected data of readings (marked with
‘v’).

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2. After setting up parameters for application DNA_RNA, press icon in the
toolbar and program will start reading datum. In the table, readings and results of
calculation will show up accordingly. Symbol ’v’ after serial numbers in table
means that the data will be printed in report. If user doesn’t want the data in the
report, please click left button of mouse in that field and the symbol will change
into ‘x’.

3. Press icon in the toolbar and user can preview the report for printing. In the

screen, icon can zoom in the report and icon can zoom out the
report.

4. In the toolbar, press icon to execute function of printing the report. During
the printing process, the default printer will be selected by system. If there is no
printer in the system, an error message will show up.
5. The contents of report for application DNA_RNA include report headers, settings
of parameters, and selected readings with results of calculation.

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Chapter 12 Function Applications-Protein Analysis

1. Select ‘Protein Analysis’ in ‘Applications’ and a function screen ‘Protein

Analysis’ will show up. A dialog box of ‘Setup Protein Analysis’ also shows up.
Press ‘OK’ after setting to execute the program according to new parameters. Or
press ‘Cancel’ to cancel settings and the program runs according to old
parameters. There are four tabs for setting in this screen: Instrument, Option,
Accessory, and Report.
a. Tab ‘Instrument’ refers to basic parameters in this application. More details
are described below:
z In ‘Assay type’, there are seven choices:
Bradford Protein Analysis
Lowry Protein Analysis-High Sensitivity
Lowry Protein Analysis-Low Sensitivity
Biuret protein Analysis
Direct UV Method
Colloidal Gold
Bicinchoninate(BCA)
z ‘No of standard replicates’ represents the standard solution number for

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 known concentration.
z ‘No of sample replicates’ represents the sample solution number for
unknown concentration.
z ‘Delay time(sec)’ represents the delay time before taking readings.
● ‘Unit’ represents concentration unit applied, totally there are 15 units
available.

b. Tab ‘Standard’ is for setting up standard solution. Details are described

below.
● Four fit types for user to choose; from left to right, 1st order equation
passing origin, 1st order equation without passing origin, 2nd order
equation passing origin, and 2nd order equation without passing origin.
● ‘Standard number’ is for user to input number of standards.
● Followed by above selections, user will key in proper values for
standards.
c. Tab ‘Accessory’ is not available in this application.
d. Tab ‘Report’ is to set up options for printing reports in this application. Please
follow explanations in previous chapter.

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2. After setting up parameters for protein analysis, press icon in the toolbar
and program will start reading standard. In the standard table, readings and
results of calculation will show up accordingly.
3. Three types of calculations are done. They are concentration obtained from fitted
equation(Cal-Conc), difference between Cal-Conc and input concentration(Diff),
and coefficient of variance from Cal-Conc(CV) for standard replicates greater
than 2.
4. Two colors in the drawings distinguish between individual data(blue) and
average data(red).

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Chapter 13 Other Functions

1. Choose ‘Open parameter file’ from ‘File’ of menu bar and a dialog box of ‘Open
parameter file’ shows up. User can select a parameter file in this screen. The file
includes all setting values for a function in a specific experiment.
2. Choose ‘Open data file’ from ‘File’ of menu bar and a dialog box of ‘Open data
file’ shows up. User can select the needed data file in this screen. The file
includes all setting values for a function and data in a specific experiment.
3. Choose ‘Save parameter file’ from ‘File’ of menu bar and it will save all setting
values for a function in the experiment.
4. Choose ‘Save data file’ from ‘File’ of menu bar and it will save all setting values
and data in a file.

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5. Open a file and the corresponding screen for the function will also be activated. If
the file is damaged or incorrect, then the file won’t be opened.
6. In ‘File format’: there are six different extension name described below:
z ‘*.pn’ means parameter file for Single cell (or plus TC).
z ‘*.pm’ menas parameter file for Multi cell.
z ‘*.ps’ means parameter file for Sipper (or plus TC).
z ‘*.dn’ means data file for Single cell (or plus TC).
z ‘*.dm’ means data file for Multi cell.
z ‘*.ds’ means data file for Sipper (or plus TC).
7. Only those files matching the accessory with the instrument can be opened.
8. The exported Excel files(*.xls) are stored in the same directory as above. Users
must go into this directory to open it for further operation.

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 APPENDIX A

- UV mate Installation and Operation -

1. PC system requirement
-CPU model need be newer than Pentium-100, equipped with CD driver.
- Microsoft Windows 98(SE) or XP.
-With minimum 12M RAM and 20Mbytes free space of hard disc drive.
-Video card capable supporting 800X600 pixels and 16 bit(Hi color) color
resolution at least.
-Mouse, keypad, one serial communication port(COM 2) for RS232 and one 9-pin
connector( one female end, one male end; all 9 pins on both ends are well
soldered).

2. About the CD-ROM

-There is one CD-ROM accompany with U2020 machine for a PC control model
you have already purchased.
-One executable program, “UV mate”, was written on CD-ROM. “UV mate” is
the main execution program for PC control model and need be resided on PC
hard disc.

3. UV mate installation
-Select the installation program”Setup.exe” under the “UV mate” directory, and
make execution.
-Please follow instructions to complete the installation work. A program
”Uvmate.exe” will be generated under C:\program files\Uvmate directory. Also
there is a data folder generated for data collection, and a few sample files are
stored inside. They can be operated even PC is not online with U2020 machine.

4. Online operation
-Make sure 9-pin connector is plugged on the RS-232 output of U2020 and
COM 2 of PC.
-Switching a standard alone U2020 machine to PC controlled machine(or vice
versa) by pressing ESC key and powering on U2020, then releasing ESC key
after hearing two beep sounds. On the bottom line of the LCD screen, you will
find a change from “Version x.x” to “Version x.x for PC”( or vice versa). After

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 around 30 seconds, a message “ Waiting for connecting….” will be shown on the
LCD display of U2020. It tells the machine has finished initialization work and
waiting for PC online connection.
-Go to Program Files of Windows 98 or XP, you will find “Irmeco” file. There
is “Uv mate” in it.
-Click “Uv mate”, PC will start running “Uvmate.exe” program.
-There is a small window titled “COM PORT SETTING” on the main window.
The connecting port was preset at COM2. You can select any com port matching
the one you connect your 9-pin connector on to.
-Click button ”Port Open”, a status message “Off-line” will change to “On-line”.
This means PC is successfully in communication with U2020.

5. Once PC is on-line with U2020 machine, the system parameters in the U2020 need
be transferred and saved in PC by the following procedure. Go to “System Setup”
from “Function” of menu bar and select “Property Setup”. A function screen
“Property Setup” will show up, then press “Measure baseline” button. After
completing this measurement, press “Save baseline”.

For a U2020 machine being first time connected to a PC, the above action is
required. Without doing that, the UVmate can not get proper measurement.
The next time after powering on the same machine again, you do not need to repeat
again unless you really want measure baseline.

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